The document profiles 1 micrometer beads under different scanning situations. It shows graphs of bead profiles with linear trendlines. The first scans were done quickly and showed periodic noise. Subsequent scans of the same bead were slower and had less noise. Waiting between scans and removing foam also reduced noise in the profiles.

1. DOG Profiles of 1um Beads under varying situations

2. -3.00E+02
-2.00E+02
-1.00E+02
0.00E+00
1.00E+02
2.00E+02
3.00E+02
0.00E+001.00E+032.00E+033.00E+034.00E+035.00E+036.00E+03
X (nm)
X (nm)
y = 0.7636x - 2386.5
R² = 0.9931
-1.50E+02
-1.00E+02
-5.00E+01
0.00E+00
5.00E+01
1.00E+02
1.50E+02
2.95E+033.00E+033.05E+033.10E+033.15E+033.20E+033.25E+033.30E+03
Series1
Linear (Series1)
The profile was taken while quickly repeatedly scanning a bead.
This bead (and the next couple) were scanned with some foam under
our slide holder.
Notice the highly periodic
nature of the noise
You can see the noise
in the overall plot

3. -3.00E+02
-2.00E+02
-1.00E+02
0.00E+00
1.00E+02
2.00E+02
3.00E+02
0.00E+001.00E+032.00E+033.00E+034.00E+035.00E+036.00E+03
X (nm)
X (nm)
y = 0.7735x - 2411.5
R² = 0.9944
-1.50E+02
-1.00E+02
-5.00E+01
0.00E+00
5.00E+01
1.00E+02
1.50E+02
2.95E+033.00E+033.05E+033.10E+033.15E+033.20E+033.25E+033.30E+03
Series1
Linear (Series1)
The profile was taken while quickly repeatedly scanning a bead.
It is the same bead as the previous profile, but the next scan.

4. The profile was taken while waiting a while before scanning a bead.
It is the same bead as the previous profile, but the next scan.
-3.00E+02
-2.00E+02
-1.00E+02
0.00E+00
1.00E+02
2.00E+02
3.00E+02
0.00E+001.00E+032.00E+033.00E+034.00E+035.00E+036.00E+03
X (nm)
X (nm)
y = 0.7794x - 2426.7
R² = 0.9984
-1.50E+02
-1.00E+02
-5.00E+01
0.00E+00
5.00E+01
1.00E+02
1.50E+02
2.95E+033.00E+033.05E+033.10E+033.15E+033.20E+033.25E+033.30E+03
Series1
Linear (Series1)
Notice the dramatic reduction in noise

5. -3.00E+02
-2.00E+02
-1.00E+02
0.00E+00
1.00E+02
2.00E+02
3.00E+02
0.00E+001.00E+032.00E+033.00E+034.00E+035.00E+036.00E+03
X (nm)
X (nm)
y = 0.7991x - 1870.4
R² = 0.9972
-1.50E+02
-1.00E+02
-5.00E+01
0.00E+00
5.00E+01
1.00E+02
1.50E+02
2.20E+032.25E+032.30E+032.35E+032.40E+032.45E+032.50E+03
Series1
Linear (Series1)
The profile was taken while waiting a while before scanning a bead.
It is the same bead as the previous profile, but scanned without
the foam.