Introduction Techniques of Plant Tissue Culture Micro propagation Stages involved in micro propagation Rooting Function of Auxin Hardening Conclusion References The term plant tissue culture broadly refers to the in vitro cultivation of plants, seeds, plant parts (tissues, embryos, single cells, protoplasts etc.) on nutrient medium under aseptic conditions. Or Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition.