This document summarizes an in vitro study of Baliospermum montanum, an endangered medicinal plant. Shoot tip cultures were used to regenerate plants, with the maximum elongation occurring in medium containing BA (5mg/l) and IBA (0.4mg/l). Nodal explants showed maximum axillary bud proliferation in medium with BA (4mg/l) and IAA (0.5mg/l). Stem explants produced the most callus when cultured in medium with BA (5mg/l) and 2,4-D (5mg/l). The study aims to develop tissue culture techniques to rapidly propagate B. montanum and protect it from overexploitation