PIPETTES
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This presentation is of - Pipettes as an instrument. Intended for Medical Students in Graduation studies as a part of syllabus only.
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Pipette updated
Types of pipettes and its applications
Micropipettes are utilized in the laboratory to transfer small quantities of liquid, usually down to 0.1 uL. They are most commonly used in chemistry, biology, forensic, pharmaceutical, and drug discovery labs, among other .
Pipettes
Pipets are used to transfer precise volumes of liquids and come in various types. Micropipets are a type of air displacement pipet that can accurately measure volumes from 0.5μl to 20ml. They allow for both forward and reverse pipetting techniques and have advantages like adjustability, ergonomic design, and preventing cross-contamination through single-use tips. Proper pipetting technique and regular calibration are important to ensure accuracy and prevent errors or contamination.
Analytical balance
Analytical balances are highly precise instruments used to determine small mass measurements. They can measure up to four decimal places of grams and are sensitive enough to detect changes as small as 0.0001 g. Due to their precision, they must be used in draft-shielded environments and powered by electricity. Analytical balances are used to weigh small quantities in the milligram range when great accuracy is required, such as pharmaceutical applications. Proper use requires leveling the balance, zeroing it, and either directly placing solid samples or taring containers for liquids or powders before taking measurements.
Tips to Perform Pipette Calibration
Pipettes are necessary equipment often used in chemical laboratories to measure and transfer specific amount of liquid.
Glassware
Laboratory glassware is usually made of borosilicate glass, which is resistant to chemicals except hydrofluoric acid. Common types of glassware include beakers for holding solutions, flasks for heating liquids, volumetric flasks for precisely measuring volumes, and pipettes for dispensing precise amounts of liquids. Pipettes can be graduated or volumetric, and are used to transfer reagents and biological samples in clinical testing.
Laboratory wares
This document provides information on laboratory glassware and plasticware, including their types, care, and uses. It discusses the characteristics of borosilicate glass used to manufacture glassware and lists precautions for handling glassware. Various types of volumetric wares like pipettes, flasks, burettes, and cylinders are described. Recommendations are provided for cleaning glassware and plasticware to ensure they are thoroughly cleaned and free of contamination.
water bath instrument
The principle used in a water bath is indirect heating. A water bath works by maintaining water at a constant temperature, which then heats other fluids placed within it through indirect contact.
The procedure for using a water bath is:
1. Fill the water bath container with clean water up to the desired level.
2. Turn the water bath on and set the thermostat to the desired temperature.
3. Allow the water to warm up until it reaches the set temperature.
4. Place the container holding the fluid you want to heat inside the water bath. The fluid will then be heated indirectly through contact with the heated water surrounding it, maintaining a constant temperature.
5. The thermostat works to
Auto analyzer
Automated analyzers have advanced diagnostic testing by increasing efficiency and accuracy while reducing human error. There are four basic approaches to automated analyzers: continuous flow analyzers, centrifugal analyzers, discrete auto analyzers, and dry chemical analyzers. Each type has its own principles and advantages such as processing multiple samples simultaneously, using small sample volumes, and eliminating manual steps. Automated analyzers have improved healthcare by providing faster, higher quality, and more standardized test results.
Recommended
Pipette updated
Types of pipettes and its applications
Micropipettes are utilized in the laboratory to transfer small quantities of liquid, usually down to 0.1 uL. They are most commonly used in chemistry, biology, forensic, pharmaceutical, and drug discovery labs, among other .
Pipettes
Pipets are used to transfer precise volumes of liquids and come in various types. Micropipets are a type of air displacement pipet that can accurately measure volumes from 0.5μl to 20ml. They allow for both forward and reverse pipetting techniques and have advantages like adjustability, ergonomic design, and preventing cross-contamination through single-use tips. Proper pipetting technique and regular calibration are important to ensure accuracy and prevent errors or contamination.
Analytical balance
Analytical balances are highly precise instruments used to determine small mass measurements. They can measure up to four decimal places of grams and are sensitive enough to detect changes as small as 0.0001 g. Due to their precision, they must be used in draft-shielded environments and powered by electricity. Analytical balances are used to weigh small quantities in the milligram range when great accuracy is required, such as pharmaceutical applications. Proper use requires leveling the balance, zeroing it, and either directly placing solid samples or taring containers for liquids or powders before taking measurements.
Tips to Perform Pipette Calibration
Pipettes are necessary equipment often used in chemical laboratories to measure and transfer specific amount of liquid.
Glassware
Laboratory glassware is usually made of borosilicate glass, which is resistant to chemicals except hydrofluoric acid. Common types of glassware include beakers for holding solutions, flasks for heating liquids, volumetric flasks for precisely measuring volumes, and pipettes for dispensing precise amounts of liquids. Pipettes can be graduated or volumetric, and are used to transfer reagents and biological samples in clinical testing.
Laboratory wares
This document provides information on laboratory glassware and plasticware, including their types, care, and uses. It discusses the characteristics of borosilicate glass used to manufacture glassware and lists precautions for handling glassware. Various types of volumetric wares like pipettes, flasks, burettes, and cylinders are described. Recommendations are provided for cleaning glassware and plasticware to ensure they are thoroughly cleaned and free of contamination.
water bath instrument
The principle used in a water bath is indirect heating. A water bath works by maintaining water at a constant temperature, which then heats other fluids placed within it through indirect contact.
The procedure for using a water bath is:
1. Fill the water bath container with clean water up to the desired level.
2. Turn the water bath on and set the thermostat to the desired temperature.
3. Allow the water to warm up until it reaches the set temperature.
4. Place the container holding the fluid you want to heat inside the water bath. The fluid will then be heated indirectly through contact with the heated water surrounding it, maintaining a constant temperature.
5. The thermostat works to
Auto analyzer
Automated analyzers have advanced diagnostic testing by increasing efficiency and accuracy while reducing human error. There are four basic approaches to automated analyzers: continuous flow analyzers, centrifugal analyzers, discrete auto analyzers, and dry chemical analyzers. Each type has its own principles and advantages such as processing multiple samples simultaneously, using small sample volumes, and eliminating manual steps. Automated analyzers have improved healthcare by providing faster, higher quality, and more standardized test results.
Biochemistry:Laboratory Safety Rules
The document outlines laboratory safety rules for students at St. Mary's College. It provides instructions on proper attire including covering legs and tying back hair. Students must wear protective equipment like eye protection and closed-toe shoes. Safety practices are described such as working under fume hoods, adding acids to water, and disposing of waste. Students are advised to never work alone and to report any accidents or unsafe conditions. Following these rules is important for protecting oneself in the laboratory environment.
Common Glassware used in Lab
Common glassware used in labs include pipettes, burettes, beakers, flasks, funnels, petri dishes, graduated cylinders, vials, slides, and stirring rods. Proper handling, cleaning, storage, and disposal of glassware is important for safety and accurate experimentation. Glassware must be carefully calibrated by determining the mass of water contained or delivered and converting to volume using water's density, in order to avoid measurement errors.
Water bath
The water bath is an
instrument used in the
laboratory for carrying out
serological, biomedical,
and pharmaceutical tests at
specific temperature ranges.
Blood collection and anticoagulants
The document discusses different methods of blood collection including capillary, venous, and arterial blood. It describes various anticoagulants used in blood collection tubes and their purposes, such as EDTA for cell counts and citrate for coagulation studies. The document also outlines the proper order for drawing blood into collection tubes and the use of blood banks for storing blood components.
Anticoagulant
This document discusses different types of anticoagulants used in blood collection and their properties. It describes how anticoagulants prevent blood from clotting by binding with calcium ions which are essential for the coagulation process. The most commonly used anticoagulant is EDTA as it best preserves cell morphology. Other anticoagulants mentioned include citrate, heparin, and oxalates. The document provides details on the preparation and use of different anticoagulants to optimize their anticoagulant effects without damaging blood cells.
Pipetting techniques
Pippettes are an essential component in fetching the accurate lab results,.... maintaining the accuracy and precision while dipensing reamins the mainstay of valid results in any test performed whether ELISA, DNA extraction Vitek, MALDI-ToF
AEC COUNT
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2. The AEC test involves diluting a blood sample with a special solution to stain and count eosinophils under a microscope.
3. An increased eosinophil count may indicate allergies, parasites, or certain types of leukemia, while a decreased count can be associated with hyperadrenalism or Cushing's syndrome.
Clinical Hematology Laboratory
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After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Automation in Biochemistry (Autoanalyzers)
Automation in biochemistry refers to using instruments to perform biochemical tests with minimal human involvement. Automated systems can perform many steps like sample handling, reagent addition, reaction incubation, and measurement that were previously done manually. The main types of automated analyzers are continuous flow analyzers, discrete autoanalyzers, and random access analyzers. Continuous flow analyzers pass samples and reagents sequentially through a single analytical pathway. Discrete autoanalyzers separate each sample and reagent in individual containers, allowing multiple tests per sample. Random access analyzers perform tests on batches of samples, selecting tests for each sample. Automated systems provide benefits like higher throughput, reduced variability, and less manual labor, but also have high initial costs.
Venous blood collection procedure
Venous blood collection, or venipuncture, allows for collection of large blood volumes from the median cubital vein in the antecubital fossa for numerous clinical tests. The procedure involves cleaning the site, applying a tourniquet, inserting a vacutainer needle to fill test tubes, removing the needle and applying pressure to stop bleeding. Proper collection permits complete blood counts, cell analyses, and other tests to aid clinical evaluation and diagnosis.
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the diiference between serum and plasma
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It then explains the principle of dry chemistry is based on reflectance spectrophotometry. Dry chemistry components use reflectance to measure color changes rather than transmission used in wet chemistry.
Examples of dry chemistry tests for urine analysis using reagent strips are provided, detecting substances like glucose, protein, blood, and pH. Dry chemistry is also used in blood tests measuring analytes like creatinine and uric acid.
Laboratory hazard
This document discusses laboratory hazards and precautions for working in a biochemical lab. It identifies three main types of laboratory hazards: physical, chemical, and biological. Specific physical hazards mentioned include fire, sharp objects, electricity, and poor housekeeping. Chemical hazards include corrosive, flammable, toxic, and carcinogenic substances. Biological hazards can arise from pathogens, animals, and body fluids. The document provides many safety precautions for each type of hazard, such as always wearing protective equipment like gloves and goggles, properly disposing of sharps and biological waste, and following general lab safety rules.
Esr
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Hemoglobin estimation
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Ph meter
A pH meter is an electronic instrument used to measure the pH (acidity or alkalinity) of liquids and semi-solids. It consists of glass and reference electrodes connected to an electronic meter that measures and displays the pH reading. The first commercial pH meter was invented in the 1930s. pH meters work by measuring the hydrogen ion concentration based on reactions in the electrodes. They must be calibrated using standard buffer solutions before use to accurately measure the pH of samples. Common applications include medicine, agriculture, food production, and more.
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PHYSIOLOGY OF CONCENTRATION OF URINE.pptx
1) The concentration of urine involves developing a medullary gradient through countercurrent mechanisms in the loop of Henle and vasa recta.
2) Sodium and other ions are actively reabsorbed up the ascending limb of Henle's loop, increasing the osmolarity of the inner medullary interstitium.
3) Urea is filtered in the glomerulus and secreted in Henle's loop, recycling between the interstitium and nephron to further concentrate the inner medulla.
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The document outlines laboratory safety rules for students at St. Mary's College. It provides instructions on proper attire including covering legs and tying back hair. Students must wear protective equipment like eye protection and closed-toe shoes. Safety practices are described such as working under fume hoods, adding acids to water, and disposing of waste. Students are advised to never work alone and to report any accidents or unsafe conditions. Following these rules is important for protecting oneself in the laboratory environment.
Common Glassware used in Lab
Common glassware used in labs include pipettes, burettes, beakers, flasks, funnels, petri dishes, graduated cylinders, vials, slides, and stirring rods. Proper handling, cleaning, storage, and disposal of glassware is important for safety and accurate experimentation. Glassware must be carefully calibrated by determining the mass of water contained or delivered and converting to volume using water's density, in order to avoid measurement errors.
Water bath
The water bath is an
instrument used in the
laboratory for carrying out
serological, biomedical,
and pharmaceutical tests at
specific temperature ranges.
Blood collection and anticoagulants
The document discusses different methods of blood collection including capillary, venous, and arterial blood. It describes various anticoagulants used in blood collection tubes and their purposes, such as EDTA for cell counts and citrate for coagulation studies. The document also outlines the proper order for drawing blood into collection tubes and the use of blood banks for storing blood components.
Anticoagulant
This document discusses different types of anticoagulants used in blood collection and their properties. It describes how anticoagulants prevent blood from clotting by binding with calcium ions which are essential for the coagulation process. The most commonly used anticoagulant is EDTA as it best preserves cell morphology. Other anticoagulants mentioned include citrate, heparin, and oxalates. The document provides details on the preparation and use of different anticoagulants to optimize their anticoagulant effects without damaging blood cells.
Pipetting techniques
Pippettes are an essential component in fetching the accurate lab results,.... maintaining the accuracy and precision while dipensing reamins the mainstay of valid results in any test performed whether ELISA, DNA extraction Vitek, MALDI-ToF
AEC COUNT
1. An absolute eosinophil count (AEC) is a blood test that measures the number of eosinophils, a type of white blood cell, to help diagnose conditions involving allergic reactions, infections, and other medical issues.
2. The AEC test involves diluting a blood sample with a special solution to stain and count eosinophils under a microscope.
3. An increased eosinophil count may indicate allergies, parasites, or certain types of leukemia, while a decreased count can be associated with hyperadrenalism or Cushing's syndrome.
Clinical Hematology Laboratory
Hematology is the branch of medicine, that is concerned with the study of blood, blood forming organs and blood diseases. It includes study of etiology, diagnosis, treatment, prognosis and prevention of blood diseases .
After the completion of this presentation we will know about:
What is hematology and its purpose.
hematology laboratory.
Blood and its compositions and collections
Hematology lab equipment's
Some hematological tests , disease and hazards too.
Automation in Biochemistry (Autoanalyzers)
Automation in biochemistry refers to using instruments to perform biochemical tests with minimal human involvement. Automated systems can perform many steps like sample handling, reagent addition, reaction incubation, and measurement that were previously done manually. The main types of automated analyzers are continuous flow analyzers, discrete autoanalyzers, and random access analyzers. Continuous flow analyzers pass samples and reagents sequentially through a single analytical pathway. Discrete autoanalyzers separate each sample and reagent in individual containers, allowing multiple tests per sample. Random access analyzers perform tests on batches of samples, selecting tests for each sample. Automated systems provide benefits like higher throughput, reduced variability, and less manual labor, but also have high initial costs.
Venous blood collection procedure
Venous blood collection, or venipuncture, allows for collection of large blood volumes from the median cubital vein in the antecubital fossa for numerous clinical tests. The procedure involves cleaning the site, applying a tourniquet, inserting a vacutainer needle to fill test tubes, removing the needle and applying pressure to stop bleeding. Proper collection permits complete blood counts, cell analyses, and other tests to aid clinical evaluation and diagnosis.
Physical examination of urine
This document discusses the physical examination of urine, including collection, preservation, and analysis methods. It describes examining the urine's volume, color, odor, appearance, pH, and specific gravity. Specific gravity is measured using a urinometer, refractometer, or dipstick. Abnormal readings may indicate conditions like diabetes, kidney disease, or urinary tract infections. The document provides details on testing urine and interpreting the results of the physical examination.
Automation in clinical hematology
The document discusses automated hematology analyzers and their advantages over manual methods. Automated analyzers can measure numerous blood parameters quickly and precisely using various techniques including electrical impedance, light scattering, fluorescence and absorption. They provide counts and indices for red blood cells, white blood cells, platelets, as well as reticulocytes. While automated analyzers offer speed, accuracy and ability to perform multiple tests, they may have issues with abnormal cells and require human examination of flags.
the diiference between serum and plasma
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APTT.pptx
The document describes the procedure for performing an activated partial thromboplastin time (APTT) test using citrated plasma. The test involves incubating plasma with brain extract, kaolin, and calcium chloride before measuring the clotting time. Prolonged APTT results indicate deficiencies in the intrinsic coagulation pathway, such as issues with factors VIII, IX, XI, or XIII; liver disease; vitamin K deficiency; or disseminated intravascular coagulation.
Dry chemistry
This document discusses dry chemistry techniques. It begins with a brief history, noting the first dry chemistry system for testing urine sugar in 1941. The key was using dried ingredients and controlling humidity.
It then explains the principle of dry chemistry is based on reflectance spectrophotometry. Dry chemistry components use reflectance to measure color changes rather than transmission used in wet chemistry.
Examples of dry chemistry tests for urine analysis using reagent strips are provided, detecting substances like glucose, protein, blood, and pH. Dry chemistry is also used in blood tests measuring analytes like creatinine and uric acid.
Laboratory hazard
This document discusses laboratory hazards and precautions for working in a biochemical lab. It identifies three main types of laboratory hazards: physical, chemical, and biological. Specific physical hazards mentioned include fire, sharp objects, electricity, and poor housekeeping. Chemical hazards include corrosive, flammable, toxic, and carcinogenic substances. Biological hazards can arise from pathogens, animals, and body fluids. The document provides many safety precautions for each type of hazard, such as always wearing protective equipment like gloves and goggles, properly disposing of sharps and biological waste, and following general lab safety rules.
Esr
The document discusses the erythrocyte sedimentation rate (ESR), including its definition, history, factors that influence it, normal values, and methods of measurement such as the Westergren and Wintrobe methods. It also describes the zeta sedimentation ratio (ZSR) and its advantages over traditional ESR measurement, such as being unaffected by anemia. The clinical significance of ESR is that it can help follow the course of disease, establish prognosis, and support diagnoses, though it is a non-specific test.
Hemoglobin estimation
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Ph meter
A pH meter is an electronic instrument used to measure the pH (acidity or alkalinity) of liquids and semi-solids. It consists of glass and reference electrodes connected to an electronic meter that measures and displays the pH reading. The first commercial pH meter was invented in the 1930s. pH meters work by measuring the hydrogen ion concentration based on reactions in the electrodes. They must be calibrated using standard buffer solutions before use to accurately measure the pH of samples. Common applications include medicine, agriculture, food production, and more.
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220711130082 Srabanti Bag Internet Resources For Natural Science
Internet resources for natural science
HYPERTENSION - SLIDE SHARE PRESENTATION.
IT WILL BE HELPFULL FOR THE NUSING STUDENTS
IT FOCUSED ON MEDICAL MANAGEMENT AND NURSING MANAGEMENT.
HIGHLIGHTS ON HEALTH EDUCATION.
Educational Technology in the Health Sciences
Plenary presentation at the NTTC Inter-university Workshop, 18 June 2024, Manila Prince Hotel.
skeleton System.pdf (skeleton system wow)
🔥🔥🔥🔥🔥🔥🔥🔥🔥
إضغ بين إيديكم من أقوى الملازم التي صممتها
ملزمة تشريح الجهاز الهيكلي (نظري 3)
💀💀💀💀💀💀💀💀💀💀
تتميز هذهِ الملزمة بعِدة مُميزات :
1- مُترجمة ترجمة تُناسب جميع المستويات
2- تحتوي على 78 رسم توضيحي لكل كلمة موجودة بالملزمة (لكل كلمة !!!!)
#فهم_ماكو_درخ
3- دقة الكتابة والصور عالية جداً جداً جداً
4- هُنالك بعض المعلومات تم توضيحها بشكل تفصيلي جداً (تُعتبر لدى الطالب أو الطالبة بإنها معلومات مُبهمة ومع ذلك تم توضيح هذهِ المعلومات المُبهمة بشكل تفصيلي جداً
5- الملزمة تشرح نفسها ب نفسها بس تكلك تعال اقراني
6- تحتوي الملزمة في اول سلايد على خارطة تتضمن جميع تفرُعات معلومات الجهاز الهيكلي المذكورة في هذهِ الملزمة
واخيراً هذهِ الملزمة حلالٌ عليكم وإتمنى منكم إن تدعولي بالخير والصحة والعافية فقط
كل التوفيق زملائي وزميلاتي ، زميلكم محمد الذهبي 💊💊
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KHUSWANT SINGH.pptx ALL YOU NEED TO KNOW ABOUT KHUSHWANT SINGH
INDIA`S OWN LITERARY GENIUS MR.KHUSHWANT SINGH WAS TRULY A VERY BRAVE SOUL AND WAS AWARDED WITH THE MAGIC OF WORDS BY GOD.
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
Slides from a Capitol Technology University webinar held June 20, 2024. The webinar featured Dr. Donovan Wright, presenting on the Department of Defense Digital Transformation.
Information and Communication Technology in Education
(𝐓𝐋𝐄 𝟏𝟎𝟎) (𝐋𝐞𝐬𝐬𝐨𝐧 2)-𝐏𝐫𝐞𝐥𝐢𝐦𝐬
𝐄𝐱𝐩𝐥𝐚𝐢𝐧 𝐭𝐡𝐞 𝐈𝐂𝐓 𝐢𝐧 𝐞𝐝𝐮𝐜𝐚𝐭𝐢𝐨𝐧:
Students will be able to explain the role and impact of Information and Communication Technology (ICT) in education. They will understand how ICT tools, such as computers, the internet, and educational software, enhance learning and teaching processes. By exploring various ICT applications, students will recognize how these technologies facilitate access to information, improve communication, support collaboration, and enable personalized learning experiences.
𝐃𝐢𝐬𝐜𝐮𝐬𝐬 𝐭𝐡𝐞 𝐫𝐞𝐥𝐢𝐚𝐛𝐥𝐞 𝐬𝐨𝐮𝐫𝐜𝐞𝐬 𝐨𝐧 𝐭𝐡𝐞 𝐢𝐧𝐭𝐞𝐫𝐧𝐞𝐭:
-Students will be able to discuss what constitutes reliable sources on the internet. They will learn to identify key characteristics of trustworthy information, such as credibility, accuracy, and authority. By examining different types of online sources, students will develop skills to evaluate the reliability of websites and content, ensuring they can distinguish between reputable information and misinformation.
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...Nguyen Thanh Tu Collection
https://app.box.com/s/qspvswamcohjtbvbbhjad04lg65waylfAccounting for Restricted Grants When and How To Record Properly
In this webinar, member learned how to stay in compliance with generally accepted accounting principles (GAAP) for restricted grants.
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Accounting for Restricted Grants When and How To Record Properly
PIPETTES
- 1. PIPETTES D r. A N IKET A . SHILWA N T Assistant Professor Sharir Kriya Dept. GJPIASR CVM University Mail ID. – ayuraniket18@gmail.com aniket.shilwant@cvmu.edu.in
- 2. INTRODUCTION A laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, from one container to other often as a media dispenser. A D V A N T A G E S Appearance – Comes in several designs. Serves for several levels of accuracy and precision as per type. Different material made – Plastic, Glass, Silica, etc. Different types – Manual, Electronic. 2
- 3. H I S T O R Y The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). 3
- 4. C L A S S I F I C A T I O N 1. Design To contain (TC) To deliver (TD) 2. Drainage characteristics Blow out Non-Blow out OR Self draining 4 Types Measuring or graduated Mohr Serological Micropipette Transfer Volumetric Pasteur pipette Automatic Micropipette
- 5. T Y P E S 1. Air displacement Micropipette 2. Volumetric pipette 3. Graduated pipette Blow out pipette Non-blow out pipette 4. Mohr pipette – Graduations always ends before tip 5. Serological pipette – Graduations runs till tip 6. Pasteur Pipette – Length depends on amount of fluid (1ml = 145mm, 2ml = 150mm) 5
- 7. L A T E S T I N N O V A T I O N Fixed channel pipette 7
- 8. Thanks! D r . A n i k e t A . S h i l w a n t Assistant Professor Sharir Kriya Dept. GJPIASR, CVM University ayuraniket18@gmail.com aniket.shilwant@cvmu.edu.in