The document provides a guide on how to perform pipette calibration, emphasizing its importance for accurate measurements in chemical laboratories. It outlines necessary equipment, steps including cleaning the pipette, measuring water weight, and adjusting for buoyancy, as well as the option of using professional calibration services for precision. Proper calibration ensures laboratory efficiency and accurate test results.

1. Tips to Perform Pipette Calibration
• Pipettes are necessary equipment often used in chemical laboratories to measure
and transfer specific amount of liquid.
• Pipettes are essentially narrow tube like equipment with a rubber bulb at the top.
• The tube is numbered from the top to the bottom mostly in increments of ten
millimeters.
• Accuracy in pipette measurement is necessary as any discrepancy in reading can
affect the result of chemical reaction.
• To ensure accuracy it is necessary to perform pipette calibration at regular
intervals.
• Calibration of equipment is desirable even in the most advanced equipment as
overtime several factors can affect the readings of measuring equipment.
• Calibration process helps to check whether or not the equipment is offering
correct reading so that it can be rectified in time and efficiency of processes or
laboratory test is maintained.

2. Given below are instructions to help you
perform pipette calibration on your own.
• Things needed:
 Beaker
 Erlenmeyer flask
 Pen and paper
 Pipette
 Pipette filler
 Thermometer
 Weight balance

3. 1.Clean the Pipette : 2. Add Distilled Water:
• Put some distilled water in an • Find out the mass of the beaker
Erlenmeyer flask. Let it stand out to the nearest tenth of a
on a bench for about 15 minutes. milligram, using a balance.
Now, measure the temperature of
the water.

4. 3. Taking Help of Pipette 4. Calculate and
Filler: determine:
• Now, using a pipette filler fill the • Calculate and determine the
pipette with the water from the mean of the four pipette
flask. Move this water into the measurements.
beaker. Weigh the beaker again.
Note down the difference in weight
of the beaker and calculate the
mass of water discharged. Carry
out this process three more times.

5. 5. Adjusting the Buoyancy 6. Calculating
of Air: Density of Water:
• Make an addition of 1.06 mg per • Calculate the density of water at the
gram to the mean mass to adjust for temperature you measured earlier in
the buoyancy of air during the the process. Determine the mean
weighing. Do not follow this step in volume of water discharged by the
case you are using a digital scale. pipette using the formula: Volume =
mass / density.

6. It is important to ensure your laboratory is clean and
organized when carrying out calibration.

7. You are not required to clean and dry the beaker following each of the
four pipette measurements as doing this will only waste your time.

8. Take help of pipette calibration service provider
• Even after taking precautions and doing all the steps correctly, there are chances
that your calibration process may not offer you precision in results. Besides, you
may not like to use your valuable resources for this purpose. A great way to help
you carry out pipette calibration work with accuracy is to take help of professional
pipette calibration service providers. Certified agencies keep an array of
sophisticated equipment and have a team of experience technicians to conduct
calibration of equipment with precision and care, thereby helping you carry out
laboratory tests with efficiency.

9. For Further Info Visit
www.RScalibration.com