Polymerase Chain Reaction (PCR) allows for targeted amplification of specific DNA sequences. PCR works by repeated cycles of heating and cooling of the DNA sample to denature the double strand into single strands and allow primers to anneal and new strands to extend. This process can generate billions of copies of the target sequence. Key components of PCR include DNA template, primers, Taq polymerase, nucleotides, and repeated thermal cycling. PCR has many applications in research and forensic analysis by enabling amplification of rare DNA sequences.
It is called “polymerase” because the only enzyme used in this reaction is DNA polymerase.
It is called “chain” because the products of the first reaction become substrates of the following one, and so on.
INTRODUCTION TO REAL TIME PCR IS GIVEN, basic principle of realtime pcr, along with the process of operating this, diagrammatic representation of the process, advantages and disadvantages o f reatimem pcr, applications of the same is also there
It is called “polymerase” because the only enzyme used in this reaction is DNA polymerase.
It is called “chain” because the products of the first reaction become substrates of the following one, and so on.
INTRODUCTION TO REAL TIME PCR IS GIVEN, basic principle of realtime pcr, along with the process of operating this, diagrammatic representation of the process, advantages and disadvantages o f reatimem pcr, applications of the same is also there
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.
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Polymerase chain reaction is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence
the speed and ease of use, sensitivity, specificity and robustness of PCR has revolutionized molecular biology and made PCR the most useful and powerful technique with great spectrum of research and diagnostic applications.
Real Time Polymerase Chain Reaction
Basics of Real Time PCR
Definition
Advantages
Principles
Instruments (Thermal Cyclers)
Useful terms
Real Time PCR Chemistry
Fluorescence Dyes
SYBR Green
EvaGreen
Melt Doctor
Fluorescence Probes
TaqMan Probe
Molecular Beacons
Scorpion Primers
SYBR Green In details
qPCR Set-Up
Assay Design
Data Analysis
Troubleshooting
Presentation on nested pcr . contain types of pcr, protocol of nested pcr, advantages of nested pcr, disadvantages of nested pcr, application of nested pcr ,pictorial representation of pcr.
this ppt contain about pcr technique and its three process,primers in pcr,dna polymerase in pcr,melting temp of dna in pcr and applications of pcr technology
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.
https://www.patreon.com/biotechlive
SUPPORT EDUCATION... SUPPORT US
Polymerase chain reaction is a technique used in molecular biology to amplify a single copy or a few copies of a segment of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence
the speed and ease of use, sensitivity, specificity and robustness of PCR has revolutionized molecular biology and made PCR the most useful and powerful technique with great spectrum of research and diagnostic applications.
Real Time Polymerase Chain Reaction
Basics of Real Time PCR
Definition
Advantages
Principles
Instruments (Thermal Cyclers)
Useful terms
Real Time PCR Chemistry
Fluorescence Dyes
SYBR Green
EvaGreen
Melt Doctor
Fluorescence Probes
TaqMan Probe
Molecular Beacons
Scorpion Primers
SYBR Green In details
qPCR Set-Up
Assay Design
Data Analysis
Troubleshooting
Presentation on nested pcr . contain types of pcr, protocol of nested pcr, advantages of nested pcr, disadvantages of nested pcr, application of nested pcr ,pictorial representation of pcr.
this ppt contain about pcr technique and its three process,primers in pcr,dna polymerase in pcr,melting temp of dna in pcr and applications of pcr technology
Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.
PCR presentation
Consists of the main parts of PCR , type of PCR including the most advanced and most efficient.
It also includes history of PCR.
PCR is a machine used to make multiple copies of gene, while gene is a part of DNA. it has 3 steps , initiaition, elongation, termination. which required different temperature for different step. these slides includes most information about PCR.
Lecture ON Polymerase Chain Reaction.
The polymerase chain reaction (PCR) is a powerful core molecular biology technique - Sometimes called "molecular photocopying. • Developed by Kary Mullis in 1985.
• It is an efficient and rapid in vitro method for enzymatic amplification of specific DNA or RNA sequences from nucleic acids of various sources. •
It generates microgram (µg) quantities of DNA copies (up to billion copies) of the desired DNA (or RNA) segment.
A simple PCR reaction consists of
i. A DNA preparation containing the desired segment to be amplified.
ii. A set of synthetic oligonucleotide primers that flank the target DNA
sequence, of about 20 bases long, specific, i.e., complementary.
iii. A thermostable DNA polymerase e.g., Taq isolated from the
bacterium Thermus acquaticus, Pfu – Pyrococcus furiosus and Vent
from Thermococcus litoralis. Pfu and Vent are more efficient than
Taq polymerase.
iv. Four deoxynucleoside triphosphate (dNTPs): TTP – thymidine
triphosphate, dCTP – deoxycyctidine triphosphate, dATP –
deoxyadenosine triphosphate and dGTP – deoxyguanosine
triphosphate
Describes the secretion and functions of Antidiuretic hormone, abnormalities associated with ADH secretion, reasons of SIADH etc in details with figures.
Second ppt on endocrine system, describing hypothalamus, pituitary and thyroid glands.
This describes the hormones from these glands and their mode of action etc
This is on the basic details of the endocrine system including the different types of hormones. It describes the mechanisms of actions of hormones. The general control mechanisms of hormone production and release are also included.
This ppt is about the variations in metabolic processes between different types of cells in different organs of our body. The reasons for the variations are also descried. This is the first set of slides on the topic.
Describes the different types of chemical messengers in mammalian body. This explains their synthesis and mode of action also. A short account of neurohormones and neuroendocrine function is also included.
This presentation is about bioenergetics. It talks about energy changes and equilibrium during different biological reactions, how exergonic and endergonic reactions are combined as sequential reactions in body, how the body system is following the law of thermodynamics etc. Role of enzymes in thermodynamics is also explained
Describes the different pathways involved in the synthesis of different eicosanoids like prostaglandins, leukotrienes, lipoxins etc along with different enzymes involved.
Describes the process of ageing in cells, factors affecting cells like telomere, free radicals, oxidative stress, DNA damage, environmental factors, proteostasis, mitochondrial disfunction etc are described
Describes various aspects of Ramachandran plot. Different torsion angles are described with clear figures. How protein folding is affected by torsion angles is also explained.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
2. Polymerase Chain Reaction
(PCR)
• Amplify a particular piece of DNA
• PCR can make billions of copies
of a target sequence of DNA in a few hours
• PCR was invented in the 1984 as a way to make
numerous copies of DNA fragments in the laboratory
• Its applications are vast and PCR is now an integral part
of Molecular Biology
3. DNA Replication vs. PCR
• Need of primers
• 95 0
C denaturation
• No Okasaki fragment formation
• DNA is synthesised from 5' to 3',
• copying the 3' to 5' strand, replication is
continuous.
• Okazaki fragments and each one requires its
own RNA primer.
4. Okasaki fragments
• In the lagging strand (the replication fork
which is travelling in the opposite direction)
synthesis occurs in small sections (100-200
nucleotides at a time in eukaryotes)
5. Principles of PCR
The PCR techniques copies the target DNA by
performing repeated cycles
Each cycle contain the following steps;
1.Denaturation/melting:
1. separate the two strands of DNA
2. At high temperature 950
C for 30s
2.Annealing:
1. Primer binding
2. 50-650
C
3.Elongation:
1. Complete the DNA molecule synthesis to the full
length
2. 70 0
C
6. Basic components in reaction
1. DNA template
2. The primers -Two primers complimentary to the 3’ end
3. Taq polymerase:
1. Heat resistant DNA polymerase
4. Deoxynucleoside Triphosphates (dNTPs)
1. CTPs
2. GTPs
3. ATPS
4. TTPs
5. Buffer
6. Divalent cations
1. Mg2+
or Mn2+
2. Mn2+
better for PCR based mutagenesis- Mn2+
increase error rate
7. Monovalent cations
K+ ions
7. DNA primers
• DNA polymerase require short stretch of
double stranded DNA
• DNA replication – short complementary
RNA formed by primases enzyme
• In PCR short stretch of DNA strand used
– Target specific
– 15-30 bases
– Determine target specificity
• Designed by using software
– Eg. Primer3
10. Key enzymes involved in DNA
Replication
• DNA Polymerase
• DNA Ligase
• Primase
• Helicase
• Topoisomerase
• Single strand binding protein
11. DNA Replication enzymes:
DNA Polyerase
• catalyzes the elongation of DNA by adding
nucleoside triphosphates to the 3’ end of the
growing strand
• A nucleotide triphosphate is a 1 sugar + 1 base + 3 phosphates
• When a nucleoside triphosphate joins the DNA strand, two
phosphates are removed.
• DNA polymerase can only add nucleotides to
3’ end of growing strand
12. Complementary Base-Pairing in DNA
• DNA is a double helix, made up of nucleotides, with
a sugar-phosphate backbone on the outside of the
helix.
• Note: a nucleotide is a sugar + phosphate + nitrogenous base
• The two strands of DNA are held together by pairs of
nitrogenous bases that are attached to each other via hydrogen
bonds.
• The nitrogenous base adenine will only pair with thymine
• The nitrogenous base guanine will only pair with cytosine
• During replication,
– DNA polymerase uses each strand as a template to synthesize new strands
of DNA with the precise, complementary order of nucleotides.
13. DNA Replication enzymes:
DNA Ligase
• The two strands of DNA in a double helix are antiparallel
(i.e. they are oriented in opposite directions with one
strand oriented from 5’ to 3’ and the other strand oriented
from 3’ to 5’
• 5’ and 3’ refer to the numbers assigned to the carbons in the 5
carbon sugar
• DNA ploymerases can only add nucleotides to the 3’ end
• One strand (referred to as the leading strand) of DNA is
synthesized continuously and
• The other strand (referred to as the lagging strand) in
synthesized in fragments (called Okazaki fragments) that
are joined together by DNA ligase.
14. DNA Replication enzymes:
Primase
• DNA Polymerase cannot initiate the
synthesis of DNA
• Remember that DNA polymerase can only add
nucleotides to 3’ end of an already existing strand of
DNA
• In humans, primase is the enzyme that can
start an RNA chain from scratch and it
creates a primer (a short stretch RNA with an
available 3’ end) that DNA polymerase can
add nucleotides to during replication.
15. DNA Replication enzymes:
Helicase, Topoisomerase and Single-strand binding
protein
• Helicase untwists the two parallel DNA strands
• Topoisomerase relieves the stress of this
twisting
• Single-strand binding protein binds to and
stabilizes the unpaired DNA strands
16. Heat-stable DNA Polymerase
• PCR involves very high temperatures-a heat-
stable DNA polymerase be used in the reaction.
• Most DNA polymerases would denature (and thus not
function properly) at the high temperatures of PCR.
• Taq DNA polymerase -from the hot springs
bacterium Thermus aquaticus in 1976
• Taq has maximal enzymatic activity at 75 °C to
80 °C, and substantially reduced activities at
lower temperatures.
17. Reaction mix
• 20 μl reaction mix in thin walled
polypropelene tubes
– 1 μl Taq polymerase (1unit)
– 1 μl primer each (forward as well as
reverse) (………………)
– 2 μl 10X buffer
– 1 μl dNTPs (………………)
– 1 μl DNA or cDNA
– 13 μl DEPC/Rnase-Dnase free water
– All the above together form 20μl
19. Step 2 Annealing or Primers Binding
Primers bind to the complimentary sequence on the
target DNA. Primers are chosen such that one is
complimentary to the one strand at one end of the
target sequence and that the other is complimentary
to the other strand at the other end of the target
sequence.
Forward Primer
Reverse Primer
20. Step 3 Extension or Primer Extension
DNA polymerase catalyzes the extension of the
strand in the 5-3 direction, starting at the
primers, attaching the appropriate nucleotide
(A-T, C-G)
extension
extension
21. • The next cycle will begin by denaturing
the new DNA strands formed in the
previous cycle
22. The Size of the DNA Fragment Produced
in PCR is Dependent on the Primers
• The PCR reaction will amplify the DNA section between the two
primers.
• If the DNA sequence is known, primers can be developed to amplify
any piece of an organism’s DNA.
Forward primer
Reverse primer
Size of fragment that is amplified
24. PCR has become a very powerful
tool in molecular biology
• One can start with a single sperm cell or
stand of hair and amplify the DNA sufficiently
to allow for DNA analysis and a distinctive
band on an agarose gel.
• One can amplify fragments of interest in an
organism’s DNA by choosing the right
primers.
• One can use the selectivity of the primers to
identify the likelihood of an individual
carrying a particular allele of a gene.
25. Selectivity of Primers
• Primers bind to their complementary sequence
on the target DNA
– A primer composed of only 3 letter, ACC, for
example, would be very likely to encounter its
complement in a genome.
– As the size of the primer is increased, the likelihood
of, for example, a primer sequence of 35 base letters
repeatedly encountering a perfect complementary
section on the target DNA become remote.
26. PCR and Disease
• Primers can be created that will only bind and
amplify certain alleles of genes or mutations of
genes
• This is the basis of genetic counseling and PCR is used
as part of the diagnostic tests for genetic diseases.
• Some diseases that can be diagnosed with the help
of PCR:
• Huntington's disease –
– CAG trinucleotide repeats less than 30 times – Normal
– More than 36 times -HD
• cystic fibrosis - CTFR mutations
• Human immunodeficiency virus
– primers that will only amplify a section of the viral DNA found in an
infected individual’s bodily fluids
27. PCR and Forensic Science
• Forensic science is the application of a broad spectrum of sciences
to answer questions of interest to the legal system. This may be in
relation to a crime or to a civil action.
• It is often of interest in forensic science to identify individuals
genetically. In these cases, one is interested in looking at variable
regions of the genome as opposed to highly-conserved genes.
• PCR can be used to amplify highly variable regions of the human
genome. These regions contain runs of short, repeated sequences
(known as variable number of tandem repeat (VNTR) sequences) .
The number of repeats can vary from 4-40 in different individuals.
• Primers are chosen that will amplify these repeated areas and the
genomic fragments generated give us a unique “genetic fingerprint”
that can be used to identify an individual.
28. PCR Applications to Forensic Science
• Paternity suits -Argentina’s Mothers of the plaza
and their search for abducted grandchildren
• Identifying badly decomposed bodies or when only
body fragments are found - World trade center,
Bosnian , Iraq & Rwandan mass graves
29. Reverse Transcriptase PCR
• ISOLATE THE mRNA/total RNA from
the cell
• Make DNA from the mRNA - reversing
“transcription”
• Use an enzyme originally obtained from
viruses–REVERSE TRANSCRIPTASE
• Complimentary DNA (cDNA) produced
30. Electrophoresis
• Agarose gel electrophoresis
• Poly acrylamide gel electrophoresis
(PAGE)
• Separation of charged molecules like
DNA and Protein
• Separation based on molecular size and
charge difference
• Pore size in the gel also affect
separation
31. DNA/RNA separation
• Phosphate group carry negatively
charged oxygen in the backbone
• This makes the DNA/RNA molecule
negatively charged
• In an electric field they move to +ve
electrode
34. Why real time PCR?
Advantages of real-time PCR
1.Amplification can be monitored
real time
2.Wider dynamic range
3.High sensitivity
4.Require very less RNA/cDNA
5.No post-PCR processing
6.Ultra rapid result
7.Highly sequence specific
35.
36. Real-time Principles
•based on the detection and quantitation of
a fluorescent reporter
•In stead of measuring the endpoint we focus on the first
significant increase in the amount of PCR product.
• The time of the increase correlates inversely to the initial
amount of DNA template
•Compare with the expression of a house keeping gene
67. A Review of Probability
A COIN THROW
The probability of a heads (H) or a tails (T) is always 0.5 for every
throw. What is the probability of getting this combination of tails in a
row?
Event Probability
Tails 0.5 = 0.5
T,T 0.5 x 0.5 = 0.25
T,T,T 0.5 x0.5 x 0.5 = 0.125
T,T,T,T,T (0.5)5
= 0.03125
T,T,T,T,T,T,T,T,T,T,T (0.5)11
= 0.0004883
T,T,T,T,T,T,T,T,T,T,T,T,T,T,T,T (0.5)16
=0.00001526
So it become increasing unlikely that one will get 16 tails in a row (1 chance in
65536 throws). In this same way, as the primer increases in size the chances of
a match other than the one intended for is highly unlikely.
68. Probability in Genetics
• There are 4 bases in the DNA molecule A,C,G,T
• The probability of encountering any of these bases in the code is 0.25 (1/4)
• So let us look at the probability of encountering a particular sequence of bases
Event Probability
A 0.25 = 0.25
A,T 0.25 x 0.25 = 0.0625
A,T,A 0.25 x0.25 x 0.25 = 0.015625
A,T,A,G,G (0.25)5
= 0.0009765
A,T,A,G,G,T,T,T,A,A,C (0.25)11
= 0.000002384
A,T,A,G,G,T,T,T,A,A,C,C,T,G,G,T (0.25)16
=0.0000000002384
So it become increasing unlikely that one will get 16 bases in this particular
sequence (1 chance in 4.3 billion). In this same way, one can see that as the
primer increases in size, the chances of a match other than the one intended
for is highly unlikely.