DNA sequencing methods have evolved from early chemical cleavage techniques developed by Maxam and Gilbert in the 1970s to the automated Sanger method in the 1980s and 1990s, leading to large scale sequencing projects like the Human Genome Project. In the 2000s, next-generation sequencing platforms like 454 pyrosequencing, Illumina, and Ion Torrent enabled massively parallel sequencing of many genomes at lower cost. These advances have allowed for widespread applications of sequencing in medicine, biology research, and other fields.