The document discusses the history and principles of nanotechnology. It describes various types of nanoparticles including inorganic nanoparticles like silver and organic nanoparticles. It explains methods for preparation of nanoparticles including physical methods like ball milling and chemical methods like cross linking microemulsion and precipitation. It discusses the effects of nanoparticles on various properties including increased surface area and reactivity. The document then summarizes several studies on the effects of nano zinc oxide, nano selenium, and nano zinc on parameters like milk production, nutrient digestibility, rumen fermentation, and semen quality in animals.
The objectives of this topic are to understand, acquire, and demonstrate the concept of nanomineral synthesis, their absorption in the body, and effect on livestock productivity.
This slides contains information on precision feeding in dairy cattle and requirement of energy, protein, fat, minerals and vitamins of a dairy cattle during lactation. Precision feeding protects reproductive health and milk production while reducing the nutrient loss in manure.
Only 25-35% of the N in feed goes into milk, with the rest excreted in feces and urine.
Dairy diets often have 120-160% of the P and that the excess is excreted in the manure.
Cost of feed can be reduced.
Precision feeding helps to improve water quality
Improving the efficiency of use of feed N.
Reduce SARA condition.
Controlled-release urea in dairy cattle feed.
Straw treatment-Ammoniation.
Reducing Enteric Methane Losses from Ruminant Livestock.
Phase feeding in dairy cattle.
Feeding bypass fat in early lactation.
Use of chelated minerals in dairy animals.
Nutraceuticals in dairy animal precision feeding.
10. Use of area specific mineral mixture to precise dairy animal nutrition.
11. TMR in precision nutrition.
12. Manipulation of dietary CAD.
Five distinct feeding phases can be defined to attain optimum production, reproduction and health of dairy cows:
Early lactation—0 to 70 days (peak milk production) after calving (postpartum).
Peak DM intake—70 to 140 days (declining milk production) postpartum.
Mid and late lactation—140 to 305 days (declining milk production) postpartum.
Dry period—60 days before the next lactation.
Transition or close-up period—14 days before to parturition.
Feed top quality forage.
Make sure the diet contains adequate amounts of CP, DIP and UIP.
Increase grain intake at a constant rate after calving.
Consider adding fat (0.4-0.6 kg/cow/day) to diets.
Allow constant access to feed.
Minimize stress conditions.
Limit urea to 80-160g/day.
Buffers, such as Na bicarbonate alone or in combination with Mg oxide (rumen pH)
In Transition period
Increase grain feeding, so cows are consuming 4.5-6 kg grain/day at calving (1% of B.wt)
Increase protein in the ration to between 14 - 15 % of the ration DM
Limit fat in the ration to 0.1kg. High fat feeding will depress DM intake.
Maintain 2.5-4kg of long hay in the ration to stimulate rumination.
Feed a low-Ca ration (< 0.20%, reduce Ca intake to 14 to 18 g/d)
Also, feed a diet with a negative dietary electrolyte balance (-10 to -15meq/100 g DM) may alleviate milk fever problems
Niacin (to control ketosis) and/or anionic salts (to help prevent milk fever) should be included in the ration during this period.
Manipulations of rumen function that can augment livestock productivity are;
Correction of concentrate to roughage ratio
Feed bypass or escaped nutrients
Defaunation of rumen
Use of yeast as probiotics
Use of anaerobic fungi
Use of other feed additives
The objectives of this topic are to understand, acquire, and demonstrate the concept of nanomineral synthesis, their absorption in the body, and effect on livestock productivity.
This slides contains information on precision feeding in dairy cattle and requirement of energy, protein, fat, minerals and vitamins of a dairy cattle during lactation. Precision feeding protects reproductive health and milk production while reducing the nutrient loss in manure.
Only 25-35% of the N in feed goes into milk, with the rest excreted in feces and urine.
Dairy diets often have 120-160% of the P and that the excess is excreted in the manure.
Cost of feed can be reduced.
Precision feeding helps to improve water quality
Improving the efficiency of use of feed N.
Reduce SARA condition.
Controlled-release urea in dairy cattle feed.
Straw treatment-Ammoniation.
Reducing Enteric Methane Losses from Ruminant Livestock.
Phase feeding in dairy cattle.
Feeding bypass fat in early lactation.
Use of chelated minerals in dairy animals.
Nutraceuticals in dairy animal precision feeding.
10. Use of area specific mineral mixture to precise dairy animal nutrition.
11. TMR in precision nutrition.
12. Manipulation of dietary CAD.
Five distinct feeding phases can be defined to attain optimum production, reproduction and health of dairy cows:
Early lactation—0 to 70 days (peak milk production) after calving (postpartum).
Peak DM intake—70 to 140 days (declining milk production) postpartum.
Mid and late lactation—140 to 305 days (declining milk production) postpartum.
Dry period—60 days before the next lactation.
Transition or close-up period—14 days before to parturition.
Feed top quality forage.
Make sure the diet contains adequate amounts of CP, DIP and UIP.
Increase grain intake at a constant rate after calving.
Consider adding fat (0.4-0.6 kg/cow/day) to diets.
Allow constant access to feed.
Minimize stress conditions.
Limit urea to 80-160g/day.
Buffers, such as Na bicarbonate alone or in combination with Mg oxide (rumen pH)
In Transition period
Increase grain feeding, so cows are consuming 4.5-6 kg grain/day at calving (1% of B.wt)
Increase protein in the ration to between 14 - 15 % of the ration DM
Limit fat in the ration to 0.1kg. High fat feeding will depress DM intake.
Maintain 2.5-4kg of long hay in the ration to stimulate rumination.
Feed a low-Ca ration (< 0.20%, reduce Ca intake to 14 to 18 g/d)
Also, feed a diet with a negative dietary electrolyte balance (-10 to -15meq/100 g DM) may alleviate milk fever problems
Niacin (to control ketosis) and/or anionic salts (to help prevent milk fever) should be included in the ration during this period.
Manipulations of rumen function that can augment livestock productivity are;
Correction of concentrate to roughage ratio
Feed bypass or escaped nutrients
Defaunation of rumen
Use of yeast as probiotics
Use of anaerobic fungi
Use of other feed additives
Different methods to calculateEnergy requirement for maintenance, growth, pregnancy, and lactation in ruminants
Sri Venkateswara veterinary university, Animal nutrition, Vishnu Vardhan Reddy
Role of Poultry in alleviating the poverty and malnutrition in IndiaBalaraj BL
The backyard poultry birds like nondescript chicken, indigenous chicken and improved birds providing high quality protein diet in the form of Egg and Poultry meat. Backyard poultry industry also helping to supplement the family income and demands zero input. The large scale commercial poultry farms (both layer and broiler) farms helping meet the meat and egg demand of the urban population.
The objective of a defined feeding management program is to supply a range of balanced diets that satisfy the nutrient requirements at all stages of development & that optimize efficiency and profitability without compromising bird welfare or the environment.
"Use of feed additives generated through fermentation technologies for livest...ExternalEvents
"Use of feed additives generated through fermentation
technologies for livestock feed " presentation by "Cavaba Srinivas Prasad, National Institute of Animal Nutrition and Physiology, Bengaluru, India"
Impact of laying hen nutrition on egg quality. Nys, Y. & Bouvarel, I. Presentation at the DSM customer event: Exploring the benefits of feed carotenoids for egg quality, Village Neuf, 2013.
The non-conventional feed resources (NCFR) refer to all those feeds that have not been traditionally used in animal feeding and or are not normally used in commercially produced rations for livestock.
NCFR include commonly, a variety of feeds from perennial crops and feeds of animal and industrial origin.
Different methods to calculateEnergy requirement for maintenance, growth, pregnancy, and lactation in ruminants
Sri Venkateswara veterinary university, Animal nutrition, Vishnu Vardhan Reddy
Role of Poultry in alleviating the poverty and malnutrition in IndiaBalaraj BL
The backyard poultry birds like nondescript chicken, indigenous chicken and improved birds providing high quality protein diet in the form of Egg and Poultry meat. Backyard poultry industry also helping to supplement the family income and demands zero input. The large scale commercial poultry farms (both layer and broiler) farms helping meet the meat and egg demand of the urban population.
The objective of a defined feeding management program is to supply a range of balanced diets that satisfy the nutrient requirements at all stages of development & that optimize efficiency and profitability without compromising bird welfare or the environment.
"Use of feed additives generated through fermentation technologies for livest...ExternalEvents
"Use of feed additives generated through fermentation
technologies for livestock feed " presentation by "Cavaba Srinivas Prasad, National Institute of Animal Nutrition and Physiology, Bengaluru, India"
Impact of laying hen nutrition on egg quality. Nys, Y. & Bouvarel, I. Presentation at the DSM customer event: Exploring the benefits of feed carotenoids for egg quality, Village Neuf, 2013.
The non-conventional feed resources (NCFR) refer to all those feeds that have not been traditionally used in animal feeding and or are not normally used in commercially produced rations for livestock.
NCFR include commonly, a variety of feeds from perennial crops and feeds of animal and industrial origin.
Pollinator Management for Organic Seed Producers
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For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
`
Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
`
Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
`
Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
`
Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
`
City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
`
Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110 ~
Nanotechnology: Basic introduction to the nanotechnology.Sathya Sujani
This simple presentation will help you to understand the every aspects of nanotechnology including basic definition and it's practical application in a very simple yet precise manner.
Here, it is a brief presentation regarding nanofertilizer, in relation to its role in enhancing the use efficiency of concerned nutrient, along with some experimrntal findings. Thank you for ur kind consideration.
Role of nanotechnology in insect pest managementbajaru
Nanotechnology is an emerging area in the field of agriculture. Nanopesticides and nanofungicides will give 100% better results when compared with the normal chemicals.
Over the past few decades, the evaluation of a number of science disciplines and technologies have revolutionized food and dairy processing sector. Most notable among these are biotechnology, information technology etc. Recently “Nanotechnology”, an essentially modern scientific field that is constantly evolving as a broad area of research, with respect to dairy and food processing, preservation, packaging and development of functional foods
In today’s competitive market new frontier technology is essential to keep leadership in the food and food processing industry. Nowadays Consumers demand fresh, authentic, convenient and flavorful food products. The future belongs to new products and new processes, with the goal of enhancing the performance of the product, prolonging the shelf life, freshness, improving the safety and quality of food product. Nanotechnology has the potential to revolutionize the food and dairy processing sectors days to come.
Nanotechnology is based on the prefix “nano”, a Greek word meaning “dwarf”. According to Pehanich (2006), nanotechnology is the understanding and control of matter at dimensions of roughly 1 to 100 nanometers. To be more specific, nanotechnology is defined as the design,production and application of structures, devices,and systems through control of the size and shape of the material at the nanometer (10-9 of a meter) scale where unique phenomenon enable novel applications (Ravichandran, 2006;National Nanotechnology Initiative, 2006).
Future prospects of nanotechnology innovations in livestock production 2019 "...Alexandria University
Future prospects of nanotechnology innovations in animal production
Ahmed Abdel-Megeed
Department of Plant Protection, Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria 21531, Egypt
Corresponding author: ahmedabdelfattah@alexu.edu.eg
Abstract
Nanotechnology is a great innovation that is revolutionizing the agricultural practices. It is a science that works at the nanoscale and provides many benefits. In this review, the fundamental concepts of nanotechnology are clarified, focusing on its primary applications and a health and environment risk assessment especially in livestock production. There is currently a lack of reliable, cost-effective diagnostic tests for early detection of diseases in farmed livestock animals. Biosensing technologies have the potential to address these problems by developing innovative diagnostic tools for the rapid detection of key health threats within the agri-food livestock sector. It also allows for greater product innovation, with the creation of new food ingredients or supplements with nanoencapsulation or nanoemulsions, achieving a slow release of some composites, or perhaps obtaining healthier foods through the improvement of organoleptic properties in the product. Although nanotechnology provides many benefits, but as with all innovations, there are disadvantages and risks associated with its use. The risk assessment must take into account that the biokinetic profile and the toxicity in the target tissues can vary depending on which nanomaterial is being referred. A risk-benefit balance on the use of nanomaterials must be carried out, and in the majority of cases, though many people are open to the advancement, more information regarding the risks is required. Above all, it must be legally regulated to guarantee Agrofood safety in all products that have been manipulated using nanotechnology.
Keywords: Nanotechnology, Livestock Production, Innovation, Risk assessment
Nanotechnology and its application in postharvest technology by l. jeebit singhJeebit Singh
A presentation on basics of Nanotechnology and its application in Postharvest Technology. A credit seminar presentation as a part of fulfillment of my Master's Degree Programme during M.Sc. 1st year 2nd semister at PG Centre, Bangalore, University of Horticultural Sciences.
Application of Nanotechnology in Agriculture with special reference to Pest M...Ramesh Kulkarni
Nanotechnology, a promising field of research opens up in the present decade a wide array of
opportunities in the present decade and is expected to give major impulses to technical innovations in
a variety of industrial sectors in the future.
Nano-coating ,Nano-treatments novel approaches to Extend the post harvest lif...Aisha Kolhar
The seminar that I have shared is Nano coating, nano treatments novel approaches for extending the post harvest life of horticulture produce. An idea how shelf of the fresh produce can be extended.
Abstract— The aim of this study has been devoted to the study of electrospun polymeric nanofiber mats that can be potentially used in active packaging. A previous characterization of the PVA solutions was carried out. Thus, density, electrical conductivity and viscosity have been measured as a function of PVA concentration (0, 4, 7 and 10% w/w). Subsequently, a standard electrospinning process was carried out. The fibber diameter was determined by analyzing high-resolution images from Scanning Electron Microscopy (SEM) using Image J software. Moreover, a characterization of tensile properties (by means of DMA) and vapour sorption capacity of PVA-based nanofiber mats was performed. In addition, water-soluble compounds were incorporated into electrospun nanofiber mats. Although they may induce marked changes in morphology, their incorporation may lead to marked improvements in techno-functional properties. Thus, addition of Sodium Carbonate (SC) involves occurrence of beads, due to the increase in electrostatic charges, whereas Citric Acid (CA) induce an increase in fibber size, related to a loss of solvent evaporation efficiency. However, both compounds significantly enhance water vapour absorption capacity.
Green nanotechnology & its application in biomedical researchRunjhunDutta
This presentation gives detailed description of Green Nanotechnology including its principles & significance. Illustrated with examples for its application in various biomedical research fields.
India is facing scarcity of feed and fodder for feeding of livestock and poultry, which limits livestock productivity. Feed and Fodder development Platform is very essential to deal with scarcity of quality feed and fodder in Livestock. Accelerated fodder production and their preservation, collection, storage and utilization of agro-industrial by-products like rice and wheat straw using bailing, cubing etc. and fodder bank may help in dealing with scarcity of fodder. Ration balancing at farmer`s doorstep, regular quality of feed and fodder will be very helpful in sustaining livestock productivity.
Antibiotic growth promoter have played a critical role in contributing to the economic effectiveness of animal production as feed supplements at sub-therapeutic doses, to improve growth and feed conversion efficiency, and to prevent infections However, injudicious use of antibiotic growth promoter leads to development of antimicrobial resistance and antibiotic residue posing a potential threat to human health.
Organic acids, probiotics, prebiiotic, enzymes, phytobiotics, bacteriophage etc. are effective antibiotic alternatives to promote animal growth performance in poultry, swine, and beef and dairy production.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
2. HISTORY
“There’s plenty of room at the bottom”
“I would like to describe a field, in which little has been done, but
in which an enormous amount can be done in principle. …..What
I want to talk about is the manipulating and controlling thing on
a small scale”
“There’s plenty of room at the bottom”
Richard Feynman, Caltech (1959)
Father of Nanotechnology
1974: Norio Taniguchi coined the term
‘Nanotechnology’ 2/61
3. Nano word is derived from the Greek nanos meaning dwarf.
Nanoparticle: Ultrafine particle of size 1-100 nm, material
with all three external dimension in the nano scale.
Nanoscience: The study of phenomena and manipulation of
material at nano scale, where properties differ significantly from
those at larger scale.
( Laurence, 2010)
Principles of Nanotechnology
3/61
7. Preparation of Nano-particles
Top down method (Physical method)
Bottom-up method (Chemical method)
Methods:
1. Physical Methods
a) Mechanical method (Ball milling)
b) Physical vapour deposition (PVD)
c) Gas phase synthesis
2. Chemical Methods:
a) Cross linking micro-emulsion
b) Precipitation (Huang et al., 2007)
7/61
8. Physical Methods
1. Mechanical method (Ball milling):
Uses different versions of mechanical dispersion viz Electro explosion,
laser-induced electro-dispersion, supersonic jets etc.
eg. Ball milling.
2. Physical vapour deposition (PVD):
Transferring the substrate to form a film by evaporation and sputtering.
In evaporation: Matters are removed from the source by thermal means.
In sputtering: Atoms or molecules are dislodged from solid target through
impact of gaseous ions.
(Cardenas et al., 2007).
3. Gas phase synthesis:
Involve atmospheric or low pressure evaporation of powders or the co-
evaporation of the two elemental components.
eg. Zinc and sulfur. Gold decorated silica nanoparticles .
(Adam et al., 2011).
8/61
9. BALL MILLING
Principle: Balls rotate with high energy inside a drum and then fall on the
solid with gravity force and crush the solid into nano crystals.
• Equipped with grinding media composed of wolfram carbide or steel.
High Energy Ball Milling (HEBM) is more efficient:
The impact energy of HEBM is 1000 times higher
To achieve desired structural changes.
Controlled milling atmosphere and temperature
A longer milling time
Use: Preparation of Nano Zinc Oxide
Drawbacks:
1. Not uniform particle size
2. Contamination during milling 9
10. 1. Cross linking micro-emulsion methods
2. Precipitation methods
CHEMICAL METHODS
Advantages of chemical methods:
Avoid contamination during physical methods
Uniform sized nano particle Production
Stabilization of nano particles from agglomeration
Surface modification and application
Processing control
Mass production.
(Lane et al., 2002) 10/61
11. Cross linking Emulsion method
Micro-emulsions are complex liquids consisting of oil,
water, surfactant (e.g. CTAB) and co-surfactant that
form a clear solution.
Micro emulsions bring together the metal precursor
(water-soluble) and the reactant (oil-soluble) to enable
the reduction of the metal to occur.
As the water concentration alters, the system can change
from a w/o to an o/w micro-emulsion.
Syntheiss of nano particles occur
(Eastoe and Warne, 1996)
11/61
12. Cross linking Emulsion
Water in oil/ oil in water emulsion preparation
Vigorous shaking
Separation & Hardening of particle
Type of Surfactant used is critical to
stability of final emulsion
12/61
13. 2.Precipitation method
Soluble form of mineral
Alkaline solution
Filtration & Centrifugation
Rinsing with hot & cold
water
2.2 g of Zn (CH3COO)2.2H2O and
2 g of NaHCO3 are mixed at room
temperature.
Pyrolyzed at 300º C for 3 h.
Zn (CH3COO)2.2H2O is changed into
ZnO nano-particles, while NaHCO3 is
changed into CH3COONa.
Washed with deionized water.
ZnO nano particles obtained by
thermal decomposition process.
(Bagum et al., 2008)
13/61
14. Surface effect
Particle size < 100nm
Lesser stability of atoms
Lesser energy needed to join adjacent atoms
Lower fusion point
Quantum effect
Special arrangement allow to have different properties
than parent element
More surface area than micro particles
Chemical reaction rate increases 1000 times
(Buzea et al., 2007)
14/61
15. Application of Nanotechnology in Animal Nutrition
Feed Biosafety
(Livestock, Environment)
Feed Quality Control
Pathogens/contaminant
Detection & Control
(Nano sensor/Biosensor)
Digestion & Absorption improvement
(Nano-particles)
Packaging/storage/Stability
(Smart packaging
Nanomaterials)
Feed supplements/
Nanocapsulation
Nanotechnolog
y
(Nano-feed)
15/61
16. ZnO-NPs inhibits growth of fungus:
Hydroxyl group of cellulose molecules of fungi
Oxygen atom of ZnO-NPs
H2O2 on the surface of ZnO-NPs
Inhibition of the fungi growth.
(Moraru et al., 2003)
Mycotoxin Binding
16/61
17. Shelf life of Feed
• Silicate nano-particles enriched films (SiO2/TiO2)
Indicate color change in presence of toxins /Microorganisms
• Prevent drying of contents
• Protection from moisture & oxidation
• Antibacterial Nano Ag/ ZnO/ MgO has repellent surfaces
• Enhanced mechanical & thermal stability
• Increases shelf life & protection
(La Coste, 2005) 17/61
18. V
V
V
V
V
V
Low particle size
More particles at
Surface
Large surface area
Higher exposure
per unit mass
Basic concept of Nanoparticles as Feed Additive
18/61
19. Nanoparticle can enter the GIT:
Directly from food & water
As feed additive & Supplements
As nano-drug
Particle uptake in GIT -
Uptake by Passive Diffusion
Through mucus and cells
Smaller particle Faster diffusion
Easily cross GIT barrier
Insoluble NPs are readily taken up across the intestinal barrier
Better absorption than macro equivalents
(Hoet et al., 2004)
19/61
20. GI Uptake and Translocation of NPs
Increases surface area available to interact with
biological support (Arbos et al., 2002)
Penetrate deeply into tissues through fine capillaries.
Efficient uptake by cells
Particles diffusion rate through GI depends on
Size & charge (Szentkuti, 1997)
Surface coating (Lai et al., 2007)
Efficient delivery of active compounds to target sites
Improve the bioavailability of Nutrients (Chen et al., 2006)
20/61
22. Additive contain minerals with a nano formulation such as
Nano Zn, Nano Se, Nano Cu , Nano Ag, etc.
Nano-additive can also be in incorporated in micelles or
capsules of protein or natural feed ingredient (Morris,2005)
Chitosan, Liposome etc. are used to protect the potency
and efficacy of oral nano-additive by-
Protecting from undesired enzymatic activity
Protecting from undesired bile salt
Protecting from commensal microorganism
Enhance bioavailability
(Handy, 2007)
22/61
28. Effect of Nano-Se and Se–Yeast in Feed Digestibility, Rumen
Fermentation in sheep
18 male sheep (42.5±3.2 kg of BW)
Control
group
3 mg Se/ kg
diet from
Nano-Se
(NS)
3 mg Se/ kg
diet from
Se-yeast
(YS)
(Shi et al., 2011)
Ration: Roughage (Alfalfa Hay+ Maize stalk) : Conc. (Maize, WB, SM, SFM):: 70: 30
Period : 20 days
28/61
29. Effects of NS and SY supplementation on ruminal pH
and fermentation in sheep (She et al., 2011)
Item Control NS YS
pH
Ammonia N (mg/100 mL)
Acetate (A) (mol/100 mol)
Propionate (P) (mol/100 mol)
Butyrate (mol/100 mol)
A/P
Total VFA (mM)
6.79c
11.05c
60.52
18.23a
6.01
3.32c
91.13a
6.34a
8.35a
58.42
21.38c
5.89
2.73b
96.41c
6.57b
9.79b
59.03
19.56b
5.92
3.02b
94.19b
29
30. Effects of NS, SY supplementation on
nutrient digestibility (She et al., 2011)
Nutrient
Digestibility
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31. Effect of Nano-Se and Se-Y on Purine Derivatives in Sheep
(She et al., 2011)
Urinary
excretion
(mmol/day)
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33. 40 Male Taihang black goats
(17.6±0.8 kg)
Age of 90±3 days
4 treatments
Period: 90 days
Effect of Nano-Se on semen quality, GPx activity, and testes
ultrastructure in male Boer goats (Shi et al., 2010)
Control
0.3 ppm
Sodium
Selenite (SS)
@0.3 ppm
Yeast- Se
(SY)
@0.3 ppm
Nano-Se
(NS)
@ 0.3 ppm
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34. Effect of Na Selenite (SS) , Yeast Se (SY) & Nano Se (NS) on
growth performance, Se concentration & antioxidant status in
growing male goats (Shi et al., 2011)
Particulars Control
0.3 ppm
SS
0.3 ppm
SY
0.3 ppm
NS
0.3 ppm
Initial Wt Kg (AVR) 17.43 17.22 17.68 17.35
Final Wt Kg (AVR) 21.92a
24.01b
25.39b 24.97b
ADG (g/d) 49.9a
75.3b
85.7c 84.7c
Blood Se(µg/ml) 90d 0.19a
0.29b
0.31b 0.38c
Serum Se (µg/ml) 90d 0.07a
0.15b
0.17b 0.21c
Liver(µg/g) 1.1a
2.5b
2.8bc 3.1c
GSH-Px(U/ml) 90d 150a
233b
291c 367d
SOD(U/ml) 90d 181a
252b
250b 313c
34
35. “Effect of elemental Nano Se on feed digestibility, rumen
fermentation & PD in goat (Shi et al., 2011)
Particulars Control
0.3 ppm
SS
0.3 ppm
SY
0.3 ppm
NS
0.3 ppm
Rumen pH 6.88 6.71 6.68 6.80
NH3N (mg%) 12.49 10.30 9.95 11.22
Propionate
mol/100mol
15.67 17.21 18.10 17.26
Total VFA (mM) 73.63 75.18 77.72 75.42
DMD 0.63 0.67 0.67 0.63
NDF dig. 0.46 0.57 0.58 0.52
CP dig. 0.64 0.71 0.72 0.64
Total PD 15.43 19.26 19.75 16.28
35/61
36. Effect of Nano-Se on semen quality, GPx activity, and testes
ultrastructure in male Boer goats (Shi et al., 2010)
42 Weaning Boer Goat buck
Two experimental treatment
Control (n=20)
@0.3 mg/kg Se
Nano selenium (n=22)
@ 0.3 mg/kg nano Se
Period: 12 weeks (Weaning to Sexual maturity)
36/61
40. Effect of Nano-Cu on growth performance & serum traits of
piglets (Gonzales Eguia et al., 2009)
36 Piglets, 4 months of age
Two experimental treatment
Period: 47 days
Control
(9.6mg/kg)
CuSO4
(50 mg/ kg)
Nano Cu
(50mg/kg)
40/61
41. Item Control
(9.6mg/kg)
CuSO4
(50 mg/ kg)
Nano Cu
(50mg/kg)
Initial Body Wt. (kg) 9.57 9.68 9.67
Final Body Wt (kg) 39.00 39.97 40.50
ADG (g) 626c
639b 656a
Feed Intake (kg/d) 0.94a
1.07b
1.04c
FCR 1.63a
1.59b 1.50c
Cu availability % 23.6c
34.2b 44.0a
Serum Cu (mg/dl) 65.8 66.1 70.1
IgG, mg/ml 41.02b
46.39a 45.17a
SOD,IU/ml 43.1c
109.0b 173.3a
Effect of Nano-Cu on Cu availability, nutrient digestibility,
growth performance & serum traits of piglets
(Gonzales Eguia et al., 2009)
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42. Effect of 20 or 40 mg/kg of silver nanoparticles on
Productive performance (5 weeks after weaning)
Control 20mg/kg 40mg/kg
Feed Intake (g /d)
0–2 weeks 154 189 148
3–5 weeks 527 670 630
Daily gain (g/d)
0–2 weeks 2.1 1.9 1.7
3–5 weeks 1.6 1.8 1.8
Feed to gain (kg/kg)
0–2 weeks 2.1 1.9 1.7
3–5 weeks 1.6 1.8 1.8
(Fondevila et al., 2009) 42/61
43. Silver nanoparticles as a potential antimicrobial additive
for weaned pigs (Fondevila et al., 2009)
Experiment 2: Effect on digestive microbiota
in vitro
Experiment 3 : Digestive microbiota
and gut morphology
(μm)
43/61
45. Effects of copper-Nanoparticles (NP-Cu) and on growth and
immunity in Broiler chicken (Wang et al., 2011)
200 broiler chicks
4 group
Basal diet with 0
(control group)
50 mg/kg of
NP-Cu
100 mg/kg of
NP-Cu
150 mg/kg of
NP-Cu
Days Maize Soybean
meal
Fish
meal
Corn gluten
meal
DCP Premi
x
ME
(MJ/kg)
CP
(%)
0-21 53 8 3.5 11.15 1.7 2.7 12.41 23.57
22-42 60 8 2.4 12.60 1.35 2.9 12.70 20.88
42 Days
46. Growth performance (0-42 days) of broilers as influenced
by the levels of NP-Cu
Particulars Control
O mg/kg
50 mg/kg
NP-Cu
100 mg/kg
NP-Cu
150 mg/kg
NP-Cu
FI (g) 92.49b
96.75a
96.71a
96.59a
ADG (g) 45.81b
48.75a
49.38a
48.73a
FCR 2.02a
1.98a
1.96a
1.98a
( Wang et al., 2011)
46/61
47. Effects of NP-Cu on haematological and micro -biota in ceacal digesta of
broiler chicken
( Wang et al.,
2011)
NP-Cu supplementation
Particulars Control 50 mg/kg 100 mg/kg 150 mg/kg
TP (g/L) 37.86b
40.91a
42.22 a
42.09a
ALB (g/L) 12.97b
14.41a
14.77a
14.34a
UN (mg/dL) 2.24a
1.99b
1.93b
1.98b
Lactobacillus
(cfu/gm)
8.16b
8.32ab
8.43a
8.34ab
Bifidobacterium
(cfu/gm)
8.31b
8.44ab
8.63a
8.52ab
Coliforms
(cfu/gm)
7.36a
7.11b
6.94bc
6.90bc
47/61
48. Effects of NP-Cu on immune organ
indexes
IMI
(mg/kg)
( Wang et al.,2011)
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49. Effects of NP-Cu on serum Ig, complements
CONC.
(g/L)
( Wang et al., 2011)
49
50. Effects of dietary Se source and level on growth
performance and Se concentration in serum and tissue of
broilers ( Hu et al.,
2012)
450 broiler chicks
5 group
Basal diet
supplement
ed with 0
(control
group)
Sodium
Selenite
@0.15
ppm
Sodium
Selenite
@0.30
ppm
Nano-Se
@0.15 ppm
Nano-Se
@0. 30 ppm
50/61
51. Effects of dietary Se source and level on growth performance and
Se concentration in serum and tissue of broilers ( Hu et al., 2012)
Particulars Control
group
Sodium
Selenite
0.15 ppm
Sodium
Selenite
0.30 ppm
Nano-Se
0.15 ppm
Nano-Se
0. 30 ppm
ADG (g/d) 44.3 50.2 49.8 50.4 51.4
Feed Intake, g/d 101.4 103 101.6 103.9 105.4
Feed Efficiency 0.44 0.49 0.49 0.49 0.49
Survial rate 85.6 96.7 98.9 96.7 96.7
Serum GSH-PX
(U/ml)
0.61 1.18 1.19 1.17 1.21
Serum Se (mg/kg) 0.05 0.09 0.14 0.11 0.18
Liver Se (mg/kg) 0.16 0.34 0.47 0.41 0.58
Kidney Se (mg/kg) 1.09 1.57 1.95 1.62 1.92
Muscle Se (mg/kg) 0.07 0.13 0.17 0.20 0.33
51/61
53. Effect of Supplementation of Different Sources of Selenium on
Humoral Immunity in Guinea Pigs
(Bunglavan and Garg , 2013)
40 male guinea pigs
(462.0 ± 9.3 g BW)
4 Groups
Control group
@ 0 Se
Nano Se
@ 150 ppb
(35 to 50 nm)
Sodium Selenite
@ 150 ppm
Organic Se
@150 ppm
Ration Composition (%)
Maize grain: 30.5
Bengal gram: 25
Wheat bran : 24
Soya bean meal: 18
Mineral mixture: 2
Common salt : 0.5
Ascorbic acid : 0.05
Period: 70 days
4 animals injected with 0.5 ml of Pasteurella multocida vaccine I/M.
Serum antibody titre determined on days 7, 14, 21 and 28 after vaccination.
53/61
54. Particular
(Days)
Control group
@ 0 Se
Nano Se
@ 150 ppb
Sodium Selenite
@ 150 ppb
Organic Se
@150 ppby
Antibody titre (Log10)
7th
day 1.ooa
1.83c
1.08a
1.45b
14th
day 1.45a
2.13b
1.90c
1.98d
21st
day 1.75a
2.66c
2.28b
2.35b
28th
day 1.68a
2.58c
2.20b
2.28b
Mean 1.47a 2.30d
1.87b
2.02c
Effect of Supplementation of Different Sources of Selenium on
Humoral Immunity in Guinea Pigs
(Bunglavan and Garg , 2013) 54/61
55. DST will invest $20 million over the five years for their
Nanomaterials Science and Technology Initiative
IVRI- Zinc & Selenium Nanoparticle as Feed additive
NAINP, Bangalore: Zn nano particle in dairy animals
AIIMS (Delhi) : Targeting and imaging of cancer
IISc (Bangalore), IIT (Mumbai) : Liposomes
NBRC (Gurgaon) : Brain tumor
Panacea Biotec (New Delhi) , Yashnanotech (Mumbai)
Dabur Research Foundation (Ghaziabad) :
Phase-1 Clinical trials of nanoparticle delivery of the
anti- cancer drug paclitaxel, mucosal drug delivery
55/61
56. Safety problem & Potential
Risks • Change in physicochemical
properties
• Change in toxico- kinetic profile
• Can cross Blood Brain Barrier
• Strong anti microbial activity
affects gut natural microflora
• Effects on cellular biochemistry &
homeostasis
• Potential for novel toxicity in GIT
• Inflammatory digestive diseases
(Zhong et al.,2008)
56/61
57. Regulations
Existing laws are inadequate to assess risks posed
by nano based foods and packaging because:
Toxicity risks remain very poorly understood
- because of their unique properties
Not assessed as new chemicals according to
many regulations
Current exposure and safety methods are not
suitable for nanomaterials.
Up to now, there is no international regulation
of nanotechnology or nano-products. 57/61
58. NANOTECHNOLOGY & ANIMAL NUTRITION: FUTURE
CONSIDERATIONS
Establishment of publicly accessible & cost
effective nano tech based feeds.
Risk Assessment & safety (Smart et al., 2006)
Legal framework governing application of
nanotechnology in feed
Legal provision to ensure safety of nano feed
(Food safety Authority of Ireland,2008)
Feed surveillance programmes
Control on disposal/recycling of nanofeed
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59. Conclusion
Nanotechnology can be used in Animal
nutrition sector to improve feed quality,
bioavailability of nutrients, growth, production
performance & immune status in livestock.
Proper legal framework & provisions to be
employed for biologically safe & cost effective
production and utilization of nano-particles for
livestock feeding . 59