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NANOTECHNOLOGY –
ITS APPLICATION IN
POST HARVEST TECHNOLOGY
Leishangthem Jeebit Singh
UHS10PGM092
PG Centre, Bangalore, UHS
03/03/2011
INTRODUCTION
“Nano” (Greek word) = “dwarf”.
“Nano” means 10-9
or one billionth of something.
Nanomaterial are the material with one, two or three
dimensions in the range 1-100 nm
DEFINITION
• The term ‘nanotechnology’ is the art and science of
manipulating matter at the nanoscale”.
Nanotechnology is research and technology at the atomic,
molecular or macromolecular levels using a length of scale
approximately 1 to 100 Nanometres, in any direction; the
creation and use of structures, devices and systems that
have novel properties and functions because of their
small size; and the ability to control and manipulate
matter on an atomic scale.
-US Environmental Protection Agency (EPA).
A nanometer is…
– one billionth of a meter
How Small Is Nanoscale?
Strand DNA Sample = 2 nm
A virus = 100nm
Atom = 1nm wide
Human hair = 80,000 nm wide
TIME LINE OF NANOTECHNOLOGY
• 1959 Feynman 1st
describe molecular machines
building with atomic precision.
‘There’s Plenty of Room at the Bottom’.
• 1974 Taniguchi uses term "nano-technology" in
paper.
• 1997 First company on nanotechnolgy founded:
Zyvex
• Presently : finding its application in nearly all the
branch of technology.
NANOTECHNOLOGY GROWTH
Table 1. Estimated government nanotechnology R&D expenditures during
1997–2003 (in U.S.$ millions/year).
• Report expected that 1 trillion USD expenditure in 2011. (nanoforum.org)
GROWTH OF NANOFOOD MARKET
Fig. 1 Estimated growth of nanofood market
NANOTECHNOLOGY IN INDIA
• Nano Science and Technology Initiative (NSTI)
• Nano Science and Technology Mission (Nano
Mission) program
• Expenditure on nanotechnology :US$ 87m
(2009).
• Among agricultural research: IARI, TNAU and
PAU (22.6 million USD in 2006).
WHAT MAKES
NANOMATERIAL
DIFFERENT?
• Reduction in size at nanoscale results in novel
properties
– Compactness
– Preciseness
– Chemical reactivity
• More surface area
– Exhibit very different
• Electrical,
• Physical (Strength, flexibilty)
• Optical (transparency, colour)
• Magnetic properties
Example of changes at Nanoscale:
• In bulk form, zinc and titanium dioxide is white
and opaque, in nano form it is transparent
• Enhanced antimicrobial property of silver
particle.
• Gold changes its colour
at different nano
scale.
Drug delivery
Imaging
Phramaceuticals
Therapy etc.
Fuel cell catalyst
Dye sensitized cell
Paint on cell
Li-ion electrodes etc.
Pollution monitoring
Scavengers
Waste treatment etc
High power magnet
Quantum computers
HD storage
E- circuits etc.
Industrial catalyst
Super plastics
Nano-inks
Nano conductors etc.
Self cleaning textile
Electric textile
Heat retention
UV blocking etc.
Nutraceuticals
Fungicides
Processing catalyst
Packaging etc.
APPLICATIO
N
Application of Nanotechnology
APPLICATION IN AGRICULTURE
• Nanocapsule
– Pesticides, fertilizers & other agrochemicals
– Vaccines
• Delivery of growth hormone in controlled fashion
• Nanosensor for
– Monitoring soil condition and crop growth
– Detection of animal and plant pathogens
– Nanochips for identity preservation and tracking
• Nanoparticles to deliver DNA to plants (targeted genetic
engineering)
• Determination of enzyme –substrate interaction.
Application of Nanotechnology in
the Post Harvest Management:
• Packaging and Food Safety
• Food processing
– Food additives
– Nano encapsulation
• Nano sensor instrument
• Quality control and testing
• Novel foods.
NANOFOOD
Food which has been
Cultivated,
Produced,
Processed or
Packaged
using nanotechnology techniques or tools,
Or, to which manufactured nanomaterials have
been added
Like --nanoparticles (iron or zinc) and
nano capsules.
IN PACKAGING AREAS
1. Improving packaging properties:
– Flexibility, gas barrier, transparency, heat
resistance, moisture barrier
1. Antimicrobial packaging and oxygen
scavenging packaging
2. Intelligent packaging
– Smart packaging, interactive packaging, e-tongue
and e-nose incorporated package etc.
1. Biodegradable packaging
IMPROVING PACKAGING PROPERTIES
• Gas barrier (O2, CO2,
H2O, Volatiles)
– Plastic polymers with
nanoclay
– Polyamide and
nanosilicate.
• Flexibility
– Nanoclays
– Polymeric resin
– Nano oxides
• UV protection
– Nanotitanium dioxide
• Mechanical strength
– Nanotitanium nitride
– Carbon nanotubules
• Surface coating
– Nanosilica
NANOCOMPOSITES FOR FOOD PACKAGING
APPLICATIONS
optimal interaction between clay and polymer
Fig2 . Different methods of developing nanocomposite.
Henriette et.al,09.
MECHANISM
• Silica : due to strong interactions between
nSiO2 and the polymer matrix via covalent
bonding.
• Clay layers: constitute a barrier to
gases and water, forcing them to
follow a tortuous path.
Table 2- Physical properties of mango puree edible films with different
concentrations of cellulose nanofibers reinforcements.
Cellulose nanofibers
(g/100g) dw
Tensile strength
(MPa)
Water vapour permeablilty
(g.mm/kPa.h.m2
)
0 (4.09 ± 0.12)e
(2.66 ± 0.06)a
1 (4.24 ± 0.25)de
(2.40 ± 0.19)ab
2 (4.42 ± 0.14)de
(2.17 ± 0.08)bc
5 (4.58 ± 0.21)cd
(2.16 ± 0.05)bc
10 (4.19 ± 0.13)c
(2.03 ± 0.11)c
18 (5.54 ± 0.07)b
(1.90 ± 0.06)cd
36 (8.76 ± 0.11)a
(1.67 ± 0.11)d
Henriette et. al., 2009
(Means in same column with different letters are significantly different at P < 0.05).
ANTIMICROBIAL PROPERTIES
• By incorporation of
– Nanosilver
– Chitosan based Ag ion.
– Starch coated with antimicrobial substances.
– Carbon nanotubules (against E. coli).
– ZnO nanoparticles.
MECHANISM FOR ANTIMICROBIAL ACTION
• Silver nanoparticle release Ag+
ions
– largely increasing permeability,
– damaging DNA.
• Nanoparticulate Chitosan: (+ve charge)
– increases membrane permeability and
– leakage of intracellular material.
• Zinc oxide nanoparticle:
– Reactive oxygen species.
Fig. 3. Exploded view of a typical antimicrobial coating
nanopackaging film.
Microorganisms hydrolyses starch based particles causing release of the
antimicrobial lysozyme resulting in inhibitors of microbial growth
Buonocore et al., 2005.
Fig. 4. Effect of different nano based packaging
material on growth of Lactobacillus plantarum on
orange juice at 56 days of storage at 4ᵒC.
(Emamifar et. al., 2011)P105 = (95% nano TiO2 + 5% nano-Ag)
Fig. 5: Effect of packaging containing nanoparticles on
the population of L. plantarum during 112 days of
storage at 4ᵒC
(Emamifar et. al., 2010)P105 = (95% nano TiO2 + 5% nano-Ag)
Fig. 6: Growth of lactic acid bacteria plotted against storage time for
fresh fruit salad coated with nanoparticle
Control
10 mg of Ag-MMT,
15 mg of Ag-MMT
20 mg of Ag-MMT.
(Costa et. al., 2011)Vertical bars indicate standard deviations.
Fig. 7 Growth of mesophilic bacteria plotted as a function of
storage time for fresh fruit salad.
Control
10 mg of Ag-MMT,
15 mg of Ag-MMT
20 mg of Ag-MMT.
(Costa et. al., 2011)
Vertical bars indicate standard deviations.
Fig. 8 Total mesophillic aerobic counts recorded in fresh-cut “Piel de
Sapo” melon pieces during 10 days shelf-life at 4 °C in the presence
1% silver nanoparticles.
Each value is the mean of three replicates and vertical bars indicate
standard deviations.
(Fernández et.al.,2010)
Fig. 9 Psychotropic microorganisms counts recorded in fresh-
cut “Piel de Sapo” melon pieces during 10 days shelf-life at
4°C in the presence silver nanoparticles.
Each value is the mean of three replicates and vertical bars indicate
standard deviations.
(Fernández et.al.,2010)
O2 SCAVENGING PACKAGING
• The incorporation of O2 scavengers into
food package can maintain very low O2
levels.
• Obtained by adding titanium nanoparticles
to different polymers.
• O2 scavenging by photocatalytic
mechanism.
INTERACTIVE/SMART/INTELLIGENT FOOD
PACKAGING
• Identify problems on its own.
• If possible, solves or indicate it.
• Interact with consumers to ‘personalise’ food,
changing colour, flavour or nutrients on
demand.
• Sense allergic to a food’s ingredients and block
the offending ingredient.
ADVANTAGES OF SMART PACKAGING
• Extend food shelf life.
• Envelope foods, preventing gas and moisture
exchange.
• Detects food spoilage and release nano-anti-
microbes.
• Nano-sensors also act as electronic barcodes.
Nano-sensors detect if packaging
• Has been opened or
tampered
• Spoiled
Intelligent MAP
• Respond to
environmental
conditions change
in moisture,
gaseous
composition and
temperature.
Fig. 10: Effects of nanocomposite based packaging on
organoleptic characteristics of kiwifruit at 4 °C storage.
◊ - Normal packaging;
▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of
nano-silver, 40% of nano-TiO2 and 25% of montmorillonite}
Hu et. al., 2011
Fig. 11: Effects of nanocomposite-based packaging on
ascorbic acid and total phenols of kiwifruit during 4 °C storage
◊ - Normal packaging;
▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of
nano-silver, 40% of nano-TiO2 and 25% of montmorillonite}
Hu et. al., 2011
Fig. 12: Effects of nanocomposite-based packaging on
reducing sugar and titratable acidity of kiwifruit during
4 °C storage
◊ - Normal packaging;
▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of
nano-silver, 40% of nano-TiO2 and 25% of montmorillonite}
Hu et. al., 2011
Table. 3: Effect on nano composite packaging on ascorbic
acid, NEB and colour of orange juice during storage.
Storage
(days)
Film type
Ascorbic acid
(mg/100g)
NEB
( OD at 420nm
Colour
difference
(∆E)
0 85.67a 0.15 h 0.00j
28
LDPE Pure 81.23c 0.24e 5.56 g
LDPE + 1.5% P105 79.87d 0.24de 6.66d
LDPE + 5% % P105 74.37 h 0.27b 6.53de
LDPE + 0.25% nano-ZnO 80.50 cd 0.23ef 6.00f
LDPE + 1% nano-ZnO 78.80e 0.25c 5.90f
56
LDPE Pure 78.40ef 0.24de 7.41c
LDPE + 1.5% P105 77.73f 0.25c 7.61b
LDPE + 5% % P105 63.90i 0.28a 7.99a
LDPE + 0.25% nano-ZnO 78.33ef 0.24d 7.48bc
LDPE + 1% nano-ZnO 76.67 g 0.25c 8.06a
(Emamifar et. al., 2010)P105 = (95% nanoTiO2 + 5% nano-Ag)
Fig. 13: Effect on nano composite packaging on sensorial
quality of orange juice after 28 days of storage.
(Emamifar et. al., 2010)
P105 = (95% nano TiO2 + 5% nano-Ag)
BIODEGRADABLE PACKAGE
• Package that can be decomposed into CO2 and
H2O by the action of naturally occurring
microorganism.
• Nano-additive derived from biomaterials
sourced from plants.
• Bionanocomposites:
– Chitosan, cellulose, collagen and zein.
Biodegradable package
Degraded by
micro-organism
CO2
FOOD PROCESSING:
• Food additives
• Encapsulation
• Fortification
• Probiotics
FOOD ADDITIVES
• Achieved by :
– By nano-emulsion,
– Surfactant micelles,
– Emulsion bilayer,
– Reverse micelles.
• Common nanoparticles.
– SiO2, TiO2, MgO, Nano calcium
salt, nano-magnesium salt,
nano-iron , synthetic nano lycopene.
Advantages of nano food
additives
–Ease of handling,
–Enhanced stability,
–Protection against oxidation,
–Retention of volatile ingredients,
–Moisture-triggered controlled release,
–pH-triggered controlled release,
–Enhanced bioavailability and efficacy
MECHANISM FOOD ADDITIVES
• Easy dispersion of water insoluble
additives (no need for emulsifier).
• Minute micelles (nanocapsules) acts as
carriers.
–essential oils, flavor, antioxidant,
vitamins, co – enzymes, minerals and
phytochemicals
NANOENCAPSULATION
• A technology of
packaging solids, liquids
or gaseous materials in
miniature, sealed
capsules that can
release their contents at
controlled rates under
specific conditions
NANOENCAPSULATION
• Nano-encapsulation offers benefits better than,
those of microencapsulation,
– Preserves the ingredients and additives during
processing and storage,
– masking unpleasant tastes and flavours,
– controlling the release of additives,
– better dispersion of water-insoluble food
ingredients and additives,
– improved uptake of the encapsulated nutrients
and supplements
• Works like a “Trojan Horse”
BASES FOR ENCAPSULATION MECHANISM
• Lipid based for antioxidant
• Soy lechitin based by nanodispersion for
– Fat solubles vit, flavour, nutraceuticals
• Liposomes based.
• Hydrophobically modified starch based.
– Bioactive compounds
Fig. 15 Inactivation curve of L. delbrueckii suspended in
orange juice treated with terpenes nanoemulsion at 32ᵒC
Control juice Juice with 1g/L terpenes nanoemulsion
5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion
Days
Donsì et. al., 2011
Fig. 16. Inactivation curve of L. delbrueckii suspended in pear
juice treated with terpenes nanoemulsion at 32ᵒC
Control juice Juice with 1g/L terpenes nanoemulsion
5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion
Donsì et. al., 2011
Fig. 17: Variation over time of the global color difference of
orange juice with terpenes nanoemulsion at 32ᵒC
Control juice Juice with 1g/L terpenes nanoemulsion
5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion
Days
Donsì et. al., 2011
Fig. 18: Variation over time of the global color difference
of pear juice with terpenes nanoemulsion at 32ᵒC
Control juice Juice with 1g/L terpenes nanoemulsion
5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion
Donsì et. al., 2011
FOOD FORTIFICATION
Fortify processed food with nano-encapsulated
nutrients,
Improves:
Increases nutrient content
Fibre -content,
Appearance and taste,
Fortifying nanoparticles of lycopene in tomato juice,
pasta sauce, and jam (Auweter et. al., 1999).
PROBIOTICS
• Live mixtures of bacterial species and can be
incorporated in foods
• Probiotic bacterial preparations could be
delivered to certain parts of the gastro-intestinal
tract where they interact with specific
receptors.
• Acts as de novo vaccines.
NANO SENSOR
• Can detect toxins and pathogens in foodstuffs in
• the lab, in the farm, during processing, storage or the shelf
• Advantages:
(a) reduced unit costs
(b) working with sample volumes in the range of nanoliters
(c) shorter analysis times
(d) multi-analyte analysis
(e) safer and environmentally friendly devices
NANOSENSOR INSTRUMENT
• Analytical instrument for
– Oxygen, ethylene, xanthin, cyanide, polyphenols
– Pesticide residue in fruits and vegetables
• Paraxon, parathion, amitrole
– Microbial contamination
• Microbes: E. coli, salmonella, vibrio
• Toxins : aflatoxin, mycotoxin
– Quality (e-nose, e–tongue )
• Vinegar, Prion protein, aflatoxin,
• F/V odour (pear, stawberry),
• Lab on chip.
GENERAL ANALYSIS
• O2 indicator (TiO2 nano composite)
• Ethylene (Tungsten oxide- tin oxide nano)
• Cyanide (Ag - NP)
• Xanthin & hypoxanthin (Nanocrystal gold-
carbon paste electrode)
• Polyphenols (Au nanoparticle modified glassy
carbon electrodes)
PESTICIDES RESIDUE ANALYSIS
• Paraxon (Organophosphorous) – chitosan
based liposome
• Parathion – ZrO2/Au
• Amitrole (herbicide) - ZnO
ASSAY USING GOLD NANOPARTICLES
(Mayra et.al, 2009)
Fig. 19: Competitive assay for organophosphorus pesticides
using gold nanoparticles
MICROBIAL NANO SENSOR
• Sensor for
– Listeria monocytogenes
– Bacillus cereus
– Mycotoxins
– E. coli
– Salmonella infantis
MECHANISM FOR MICROBIAL DETECTION
• Antibody
–Nanoparticle complex
–Fluorescent & detection
–Conjugation with magnetic nanoparticles.
• Protein binds with nanoparticle having
flurosecent property
DIRECT BACTERIAL DETECTION
NANOBASED IMMUNOASSAY FOR AFLATOXIN
Major food and agriculture companies engaged
in nanotechnology R&D
• Altria (Kraft Foods)
• Associated British Foods
• BASF
• Bayer
• Cadbury Schweppes
• Campbell Soup
• DuPont Food Industry
Solutions
• General Mills
• Krafte
• Glaxo-SmithKline
• Goodman Fielder
• Group Danone
• John Lust Group Plc
• Nestlé
• Nichirei
• Nippon Suisan Kaisha
• PepsiCo
• Unilever
• United
Table 4 :Commercial products in market (Packaging)
Product name Manufacturer Nano content Claim
Durethan KU 2-
2601
Bayer Silica in a polymer-
based
nanocomposite
Prevent the
penetration of oxygen
Nanoplastic wrap SongSing
nanotechnology
Nano zinc Antibacterial, anti-uv,
temperature resistant
Cadbury
Schweppes
Plantic
Technologies
Thermoformed
Plantic®
R1 trays
Biodegradable after
use
Mark and spencer Plantic
Technologies
Plantic Plastics Biodegradable after
use
DuPont™ Light
Stabilizer 210
DuPont Nano titanium
dioxide
U.V.-protected plastic
food packaging
Nano Silver Food
Storage
Containers
Nano Silver
Wholesale
Ltd.
Nano Silver Antimicrobial
food containers
Table 5: Commercial products available in market
(food and beverages)
Product name Manufacturer Nano content Claim
NanoTea Shenzen Become
Industry &
Trading Co.
Nanoparticles Se enriched.
Fortified fruit juice High Vive.com 300nm iron
(SunActiveFe)
“Daily Vitamin
Boost”
Fortified fruit juice
Jamba Juice
Hawaii
300nm iron
(SunActive Fe)
22 essential vitamins and
minerals.
Oat Vanilla
Nutritional
Drink Mix
Toddler Health 300nm iron
(SunActive Fe)
Balanced nutritional drink
for children from 13
months to 5 years.
Table 6: Commercial products available in market
(food additives)
Product name Manufacturer Nano content Claim
Solu™ E 200 BASF Vitamin E nano-
solution
using NovaSol
Solubilsates of fat-soluble
vitamins
Synthetic lycopene BASF LycoVit 10% (< 200nm
synthetic lycopene)
Nano-self assemble
structured liquids
(NSSL)
Nutralease Nano micelles for
encapsulation of
nutraceuticals
Improved bioavailability
for nutraceuticals
NanoCoQ10® Pharmanex Nano coQ10 Nano technology to deliver
highly bioavailable
coenzyme Q10
AquaNova NovaSol Aquanova Product micelle
(capsule)
An optimum carrier system
of hydrophobic
substances.
WHY NANOPARTICLES POSE NEW
RISKS
• Chemically reactive than larger particles
• Greater access to our bodies than larger particles
• Greater bioavailability and greater bioactivity may
introduce new toxicity risks
• Compromise our immune system response
• Easily dispersible in environment.
REGULATION
• Nano food products are already
in market.
• The need for a stringent nano
regulation is felt.
• Formulation of regulations
under the process.
• But there are still no laws.
Conclusion
• Incorporation of Ag ions, TiO2 and ZnO has been
successfully carried out for their antimicrobial
property in packaging.
• Nano clay and monmorollite are usually used as
based for packaging.
• Encapsulation with terpene oil was also successful
for quality improvement.
• Commercial food and beverages products based on
nano Fe, nano Se and nanoencapsulation are already
in market.
THE NEXT BIG THING IS
REALLY SMALL.
Thank YouThank You.

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Nanotechnology and its application in postharvest technology by l. jeebit singh

  • 1. NANOTECHNOLOGY – ITS APPLICATION IN POST HARVEST TECHNOLOGY Leishangthem Jeebit Singh UHS10PGM092 PG Centre, Bangalore, UHS 03/03/2011
  • 2. INTRODUCTION “Nano” (Greek word) = “dwarf”. “Nano” means 10-9 or one billionth of something. Nanomaterial are the material with one, two or three dimensions in the range 1-100 nm
  • 3. DEFINITION • The term ‘nanotechnology’ is the art and science of manipulating matter at the nanoscale”. Nanotechnology is research and technology at the atomic, molecular or macromolecular levels using a length of scale approximately 1 to 100 Nanometres, in any direction; the creation and use of structures, devices and systems that have novel properties and functions because of their small size; and the ability to control and manipulate matter on an atomic scale. -US Environmental Protection Agency (EPA).
  • 4. A nanometer is… – one billionth of a meter How Small Is Nanoscale? Strand DNA Sample = 2 nm A virus = 100nm Atom = 1nm wide Human hair = 80,000 nm wide
  • 5. TIME LINE OF NANOTECHNOLOGY • 1959 Feynman 1st describe molecular machines building with atomic precision. ‘There’s Plenty of Room at the Bottom’. • 1974 Taniguchi uses term "nano-technology" in paper. • 1997 First company on nanotechnolgy founded: Zyvex • Presently : finding its application in nearly all the branch of technology.
  • 6. NANOTECHNOLOGY GROWTH Table 1. Estimated government nanotechnology R&D expenditures during 1997–2003 (in U.S.$ millions/year). • Report expected that 1 trillion USD expenditure in 2011. (nanoforum.org)
  • 7. GROWTH OF NANOFOOD MARKET Fig. 1 Estimated growth of nanofood market
  • 8. NANOTECHNOLOGY IN INDIA • Nano Science and Technology Initiative (NSTI) • Nano Science and Technology Mission (Nano Mission) program • Expenditure on nanotechnology :US$ 87m (2009). • Among agricultural research: IARI, TNAU and PAU (22.6 million USD in 2006).
  • 9. WHAT MAKES NANOMATERIAL DIFFERENT? • Reduction in size at nanoscale results in novel properties – Compactness – Preciseness – Chemical reactivity • More surface area – Exhibit very different • Electrical, • Physical (Strength, flexibilty) • Optical (transparency, colour) • Magnetic properties
  • 10. Example of changes at Nanoscale: • In bulk form, zinc and titanium dioxide is white and opaque, in nano form it is transparent • Enhanced antimicrobial property of silver particle. • Gold changes its colour at different nano scale.
  • 11. Drug delivery Imaging Phramaceuticals Therapy etc. Fuel cell catalyst Dye sensitized cell Paint on cell Li-ion electrodes etc. Pollution monitoring Scavengers Waste treatment etc High power magnet Quantum computers HD storage E- circuits etc. Industrial catalyst Super plastics Nano-inks Nano conductors etc. Self cleaning textile Electric textile Heat retention UV blocking etc. Nutraceuticals Fungicides Processing catalyst Packaging etc. APPLICATIO N
  • 13. APPLICATION IN AGRICULTURE • Nanocapsule – Pesticides, fertilizers & other agrochemicals – Vaccines • Delivery of growth hormone in controlled fashion • Nanosensor for – Monitoring soil condition and crop growth – Detection of animal and plant pathogens – Nanochips for identity preservation and tracking • Nanoparticles to deliver DNA to plants (targeted genetic engineering) • Determination of enzyme –substrate interaction.
  • 14. Application of Nanotechnology in the Post Harvest Management: • Packaging and Food Safety • Food processing – Food additives – Nano encapsulation • Nano sensor instrument • Quality control and testing • Novel foods.
  • 15. NANOFOOD Food which has been Cultivated, Produced, Processed or Packaged using nanotechnology techniques or tools, Or, to which manufactured nanomaterials have been added Like --nanoparticles (iron or zinc) and nano capsules.
  • 16. IN PACKAGING AREAS 1. Improving packaging properties: – Flexibility, gas barrier, transparency, heat resistance, moisture barrier 1. Antimicrobial packaging and oxygen scavenging packaging 2. Intelligent packaging – Smart packaging, interactive packaging, e-tongue and e-nose incorporated package etc. 1. Biodegradable packaging
  • 17. IMPROVING PACKAGING PROPERTIES • Gas barrier (O2, CO2, H2O, Volatiles) – Plastic polymers with nanoclay – Polyamide and nanosilicate. • Flexibility – Nanoclays – Polymeric resin – Nano oxides • UV protection – Nanotitanium dioxide • Mechanical strength – Nanotitanium nitride – Carbon nanotubules • Surface coating – Nanosilica
  • 18. NANOCOMPOSITES FOR FOOD PACKAGING APPLICATIONS optimal interaction between clay and polymer Fig2 . Different methods of developing nanocomposite. Henriette et.al,09.
  • 19. MECHANISM • Silica : due to strong interactions between nSiO2 and the polymer matrix via covalent bonding. • Clay layers: constitute a barrier to gases and water, forcing them to follow a tortuous path.
  • 20. Table 2- Physical properties of mango puree edible films with different concentrations of cellulose nanofibers reinforcements. Cellulose nanofibers (g/100g) dw Tensile strength (MPa) Water vapour permeablilty (g.mm/kPa.h.m2 ) 0 (4.09 ± 0.12)e (2.66 ± 0.06)a 1 (4.24 ± 0.25)de (2.40 ± 0.19)ab 2 (4.42 ± 0.14)de (2.17 ± 0.08)bc 5 (4.58 ± 0.21)cd (2.16 ± 0.05)bc 10 (4.19 ± 0.13)c (2.03 ± 0.11)c 18 (5.54 ± 0.07)b (1.90 ± 0.06)cd 36 (8.76 ± 0.11)a (1.67 ± 0.11)d Henriette et. al., 2009 (Means in same column with different letters are significantly different at P < 0.05).
  • 21. ANTIMICROBIAL PROPERTIES • By incorporation of – Nanosilver – Chitosan based Ag ion. – Starch coated with antimicrobial substances. – Carbon nanotubules (against E. coli). – ZnO nanoparticles.
  • 22. MECHANISM FOR ANTIMICROBIAL ACTION • Silver nanoparticle release Ag+ ions – largely increasing permeability, – damaging DNA. • Nanoparticulate Chitosan: (+ve charge) – increases membrane permeability and – leakage of intracellular material. • Zinc oxide nanoparticle: – Reactive oxygen species.
  • 23. Fig. 3. Exploded view of a typical antimicrobial coating nanopackaging film. Microorganisms hydrolyses starch based particles causing release of the antimicrobial lysozyme resulting in inhibitors of microbial growth Buonocore et al., 2005.
  • 24. Fig. 4. Effect of different nano based packaging material on growth of Lactobacillus plantarum on orange juice at 56 days of storage at 4ᵒC. (Emamifar et. al., 2011)P105 = (95% nano TiO2 + 5% nano-Ag)
  • 25. Fig. 5: Effect of packaging containing nanoparticles on the population of L. plantarum during 112 days of storage at 4ᵒC (Emamifar et. al., 2010)P105 = (95% nano TiO2 + 5% nano-Ag)
  • 26. Fig. 6: Growth of lactic acid bacteria plotted against storage time for fresh fruit salad coated with nanoparticle Control 10 mg of Ag-MMT, 15 mg of Ag-MMT 20 mg of Ag-MMT. (Costa et. al., 2011)Vertical bars indicate standard deviations.
  • 27. Fig. 7 Growth of mesophilic bacteria plotted as a function of storage time for fresh fruit salad. Control 10 mg of Ag-MMT, 15 mg of Ag-MMT 20 mg of Ag-MMT. (Costa et. al., 2011) Vertical bars indicate standard deviations.
  • 28. Fig. 8 Total mesophillic aerobic counts recorded in fresh-cut “Piel de Sapo” melon pieces during 10 days shelf-life at 4 °C in the presence 1% silver nanoparticles. Each value is the mean of three replicates and vertical bars indicate standard deviations. (Fernández et.al.,2010)
  • 29. Fig. 9 Psychotropic microorganisms counts recorded in fresh- cut “Piel de Sapo” melon pieces during 10 days shelf-life at 4°C in the presence silver nanoparticles. Each value is the mean of three replicates and vertical bars indicate standard deviations. (Fernández et.al.,2010)
  • 30. O2 SCAVENGING PACKAGING • The incorporation of O2 scavengers into food package can maintain very low O2 levels. • Obtained by adding titanium nanoparticles to different polymers. • O2 scavenging by photocatalytic mechanism.
  • 31. INTERACTIVE/SMART/INTELLIGENT FOOD PACKAGING • Identify problems on its own. • If possible, solves or indicate it. • Interact with consumers to ‘personalise’ food, changing colour, flavour or nutrients on demand. • Sense allergic to a food’s ingredients and block the offending ingredient.
  • 32. ADVANTAGES OF SMART PACKAGING • Extend food shelf life. • Envelope foods, preventing gas and moisture exchange. • Detects food spoilage and release nano-anti- microbes. • Nano-sensors also act as electronic barcodes.
  • 33. Nano-sensors detect if packaging • Has been opened or tampered • Spoiled
  • 34. Intelligent MAP • Respond to environmental conditions change in moisture, gaseous composition and temperature.
  • 35. Fig. 10: Effects of nanocomposite based packaging on organoleptic characteristics of kiwifruit at 4 °C storage. ◊ - Normal packaging; ▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of nano-silver, 40% of nano-TiO2 and 25% of montmorillonite} Hu et. al., 2011
  • 36. Fig. 11: Effects of nanocomposite-based packaging on ascorbic acid and total phenols of kiwifruit during 4 °C storage ◊ - Normal packaging; ▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of nano-silver, 40% of nano-TiO2 and 25% of montmorillonite} Hu et. al., 2011
  • 37. Fig. 12: Effects of nanocomposite-based packaging on reducing sugar and titratable acidity of kiwifruit during 4 °C storage ◊ - Normal packaging; ▲- Polyethylene(70%) blended with nanoparticle (30%) {35% of nano-silver, 40% of nano-TiO2 and 25% of montmorillonite} Hu et. al., 2011
  • 38. Table. 3: Effect on nano composite packaging on ascorbic acid, NEB and colour of orange juice during storage. Storage (days) Film type Ascorbic acid (mg/100g) NEB ( OD at 420nm Colour difference (∆E) 0 85.67a 0.15 h 0.00j 28 LDPE Pure 81.23c 0.24e 5.56 g LDPE + 1.5% P105 79.87d 0.24de 6.66d LDPE + 5% % P105 74.37 h 0.27b 6.53de LDPE + 0.25% nano-ZnO 80.50 cd 0.23ef 6.00f LDPE + 1% nano-ZnO 78.80e 0.25c 5.90f 56 LDPE Pure 78.40ef 0.24de 7.41c LDPE + 1.5% P105 77.73f 0.25c 7.61b LDPE + 5% % P105 63.90i 0.28a 7.99a LDPE + 0.25% nano-ZnO 78.33ef 0.24d 7.48bc LDPE + 1% nano-ZnO 76.67 g 0.25c 8.06a (Emamifar et. al., 2010)P105 = (95% nanoTiO2 + 5% nano-Ag)
  • 39. Fig. 13: Effect on nano composite packaging on sensorial quality of orange juice after 28 days of storage. (Emamifar et. al., 2010) P105 = (95% nano TiO2 + 5% nano-Ag)
  • 40. BIODEGRADABLE PACKAGE • Package that can be decomposed into CO2 and H2O by the action of naturally occurring microorganism. • Nano-additive derived from biomaterials sourced from plants. • Bionanocomposites: – Chitosan, cellulose, collagen and zein.
  • 42. FOOD PROCESSING: • Food additives • Encapsulation • Fortification • Probiotics
  • 43. FOOD ADDITIVES • Achieved by : – By nano-emulsion, – Surfactant micelles, – Emulsion bilayer, – Reverse micelles. • Common nanoparticles. – SiO2, TiO2, MgO, Nano calcium salt, nano-magnesium salt, nano-iron , synthetic nano lycopene.
  • 44. Advantages of nano food additives –Ease of handling, –Enhanced stability, –Protection against oxidation, –Retention of volatile ingredients, –Moisture-triggered controlled release, –pH-triggered controlled release, –Enhanced bioavailability and efficacy
  • 45. MECHANISM FOOD ADDITIVES • Easy dispersion of water insoluble additives (no need for emulsifier). • Minute micelles (nanocapsules) acts as carriers. –essential oils, flavor, antioxidant, vitamins, co – enzymes, minerals and phytochemicals
  • 46. NANOENCAPSULATION • A technology of packaging solids, liquids or gaseous materials in miniature, sealed capsules that can release their contents at controlled rates under specific conditions
  • 47. NANOENCAPSULATION • Nano-encapsulation offers benefits better than, those of microencapsulation, – Preserves the ingredients and additives during processing and storage, – masking unpleasant tastes and flavours, – controlling the release of additives, – better dispersion of water-insoluble food ingredients and additives, – improved uptake of the encapsulated nutrients and supplements • Works like a “Trojan Horse”
  • 48.
  • 49. BASES FOR ENCAPSULATION MECHANISM • Lipid based for antioxidant • Soy lechitin based by nanodispersion for – Fat solubles vit, flavour, nutraceuticals • Liposomes based. • Hydrophobically modified starch based. – Bioactive compounds
  • 50. Fig. 15 Inactivation curve of L. delbrueckii suspended in orange juice treated with terpenes nanoemulsion at 32ᵒC Control juice Juice with 1g/L terpenes nanoemulsion 5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion Days Donsì et. al., 2011
  • 51. Fig. 16. Inactivation curve of L. delbrueckii suspended in pear juice treated with terpenes nanoemulsion at 32ᵒC Control juice Juice with 1g/L terpenes nanoemulsion 5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion Donsì et. al., 2011
  • 52. Fig. 17: Variation over time of the global color difference of orange juice with terpenes nanoemulsion at 32ᵒC Control juice Juice with 1g/L terpenes nanoemulsion 5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion Days Donsì et. al., 2011
  • 53. Fig. 18: Variation over time of the global color difference of pear juice with terpenes nanoemulsion at 32ᵒC Control juice Juice with 1g/L terpenes nanoemulsion 5g/L terpenes nanoemulsion 10 g/L terpenes nanoemulsion Donsì et. al., 2011
  • 54. FOOD FORTIFICATION Fortify processed food with nano-encapsulated nutrients, Improves: Increases nutrient content Fibre -content, Appearance and taste, Fortifying nanoparticles of lycopene in tomato juice, pasta sauce, and jam (Auweter et. al., 1999).
  • 55. PROBIOTICS • Live mixtures of bacterial species and can be incorporated in foods • Probiotic bacterial preparations could be delivered to certain parts of the gastro-intestinal tract where they interact with specific receptors. • Acts as de novo vaccines.
  • 56. NANO SENSOR • Can detect toxins and pathogens in foodstuffs in • the lab, in the farm, during processing, storage or the shelf • Advantages: (a) reduced unit costs (b) working with sample volumes in the range of nanoliters (c) shorter analysis times (d) multi-analyte analysis (e) safer and environmentally friendly devices
  • 57. NANOSENSOR INSTRUMENT • Analytical instrument for – Oxygen, ethylene, xanthin, cyanide, polyphenols – Pesticide residue in fruits and vegetables • Paraxon, parathion, amitrole – Microbial contamination • Microbes: E. coli, salmonella, vibrio • Toxins : aflatoxin, mycotoxin – Quality (e-nose, e–tongue ) • Vinegar, Prion protein, aflatoxin, • F/V odour (pear, stawberry), • Lab on chip.
  • 58. GENERAL ANALYSIS • O2 indicator (TiO2 nano composite) • Ethylene (Tungsten oxide- tin oxide nano) • Cyanide (Ag - NP) • Xanthin & hypoxanthin (Nanocrystal gold- carbon paste electrode) • Polyphenols (Au nanoparticle modified glassy carbon electrodes)
  • 59. PESTICIDES RESIDUE ANALYSIS • Paraxon (Organophosphorous) – chitosan based liposome • Parathion – ZrO2/Au • Amitrole (herbicide) - ZnO
  • 60. ASSAY USING GOLD NANOPARTICLES (Mayra et.al, 2009) Fig. 19: Competitive assay for organophosphorus pesticides using gold nanoparticles
  • 61. MICROBIAL NANO SENSOR • Sensor for – Listeria monocytogenes – Bacillus cereus – Mycotoxins – E. coli – Salmonella infantis
  • 62. MECHANISM FOR MICROBIAL DETECTION • Antibody –Nanoparticle complex –Fluorescent & detection –Conjugation with magnetic nanoparticles. • Protein binds with nanoparticle having flurosecent property
  • 65. Major food and agriculture companies engaged in nanotechnology R&D • Altria (Kraft Foods) • Associated British Foods • BASF • Bayer • Cadbury Schweppes • Campbell Soup • DuPont Food Industry Solutions • General Mills • Krafte • Glaxo-SmithKline • Goodman Fielder • Group Danone • John Lust Group Plc • Nestlé • Nichirei • Nippon Suisan Kaisha • PepsiCo • Unilever • United
  • 66. Table 4 :Commercial products in market (Packaging) Product name Manufacturer Nano content Claim Durethan KU 2- 2601 Bayer Silica in a polymer- based nanocomposite Prevent the penetration of oxygen Nanoplastic wrap SongSing nanotechnology Nano zinc Antibacterial, anti-uv, temperature resistant Cadbury Schweppes Plantic Technologies Thermoformed Plantic® R1 trays Biodegradable after use Mark and spencer Plantic Technologies Plantic Plastics Biodegradable after use DuPont™ Light Stabilizer 210 DuPont Nano titanium dioxide U.V.-protected plastic food packaging Nano Silver Food Storage Containers Nano Silver Wholesale Ltd. Nano Silver Antimicrobial food containers
  • 67. Table 5: Commercial products available in market (food and beverages) Product name Manufacturer Nano content Claim NanoTea Shenzen Become Industry & Trading Co. Nanoparticles Se enriched. Fortified fruit juice High Vive.com 300nm iron (SunActiveFe) “Daily Vitamin Boost” Fortified fruit juice Jamba Juice Hawaii 300nm iron (SunActive Fe) 22 essential vitamins and minerals. Oat Vanilla Nutritional Drink Mix Toddler Health 300nm iron (SunActive Fe) Balanced nutritional drink for children from 13 months to 5 years.
  • 68. Table 6: Commercial products available in market (food additives) Product name Manufacturer Nano content Claim Solu™ E 200 BASF Vitamin E nano- solution using NovaSol Solubilsates of fat-soluble vitamins Synthetic lycopene BASF LycoVit 10% (< 200nm synthetic lycopene) Nano-self assemble structured liquids (NSSL) Nutralease Nano micelles for encapsulation of nutraceuticals Improved bioavailability for nutraceuticals NanoCoQ10® Pharmanex Nano coQ10 Nano technology to deliver highly bioavailable coenzyme Q10 AquaNova NovaSol Aquanova Product micelle (capsule) An optimum carrier system of hydrophobic substances.
  • 69. WHY NANOPARTICLES POSE NEW RISKS • Chemically reactive than larger particles • Greater access to our bodies than larger particles • Greater bioavailability and greater bioactivity may introduce new toxicity risks • Compromise our immune system response • Easily dispersible in environment.
  • 70. REGULATION • Nano food products are already in market. • The need for a stringent nano regulation is felt. • Formulation of regulations under the process. • But there are still no laws.
  • 71. Conclusion • Incorporation of Ag ions, TiO2 and ZnO has been successfully carried out for their antimicrobial property in packaging. • Nano clay and monmorollite are usually used as based for packaging. • Encapsulation with terpene oil was also successful for quality improvement. • Commercial food and beverages products based on nano Fe, nano Se and nanoencapsulation are already in market.
  • 72. THE NEXT BIG THING IS REALLY SMALL. Thank YouThank You.

Editor's Notes

  1. O2 responsible for various metabbolic activity