Mass spectrometry is a technique that uses the deflection of charged particles by a magnetic field to determine the relative masses of molecular ions and fragments. It provides a great deal of information from small samples and can be used to determine molecular mass, structure, and purity. Various ionization sources like electron ionization, chemical ionization, fast atom bombardment, and matrix-assisted laser desorption/ionization are used to vaporize and ionize samples for analysis in mass analyzers such as quadrupoles, ion traps, and time-of-flight instruments. Mass spectra provide the abundance of ions as a function of their mass-to-charge ratio and can reveal molecular structure through characteristic fragmentation patterns.
Photoelectric Effect and Photochemical Reactions. Photons of Light and Chemical Reactions. photodissociation of O2. It was found that Stopping voltage is proportional to the frequency of the incident light but independent of the light intensity
Raman Spectroscopy - Principle, Criteria, Instrumentation and ApplicationsPrabha Nagarajan
Basic principle of Raman scattering- Difference between Rayleigh and Raman Scattering- Major criteria for Raman active in compounds,-Stroke's lines and Anti-stoke lines- Difference and between IR and Raman spectroscopy- Wide applications of Raman spectroscopy.
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Photoelectric Effect and Photochemical Reactions. Photons of Light and Chemical Reactions. photodissociation of O2. It was found that Stopping voltage is proportional to the frequency of the incident light but independent of the light intensity
Raman Spectroscopy - Principle, Criteria, Instrumentation and ApplicationsPrabha Nagarajan
Basic principle of Raman scattering- Difference between Rayleigh and Raman Scattering- Major criteria for Raman active in compounds,-Stroke's lines and Anti-stoke lines- Difference and between IR and Raman spectroscopy- Wide applications of Raman spectroscopy.
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Photoelectron spectroscopy
- a single photon in/ electron out process
• X-ray Photoelectron Spectroscopy (XPS)
- using soft x-ray (200-2000 eV) radiation to
examine core-levels.
• Ultraviolet Photoelectron Spectroscopy (UPS)
- using vacuum UV (10-45 eV) radiation to
examine valence levels.
This slide discusses the principle, instrumentation, process, detectors, sample ,solvents used in mass spectroscopy and also its applications and limitations.
MASS SPECTROSCOPY ( Molecular ion, Base peak, Isotopic abundance, Metastable ...Sachin Kale
CONTENT:
Molecular Ion Peak
Significance of Molecular ion & Graphically Method
Base Peak
Isotopic Abundance
Metastable Ion
Significance of Metastable ion
Nitrogen Rule & graphs
Formulation of Rule
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
TYPES OF PEAKS IN MASS SPECTROSCOPY.pptxAnupamaCp2
Types of peaks in mass spectroscopy.
Molecular ion or parent peak.
base peak.
fragment ions.
rearrangement ion.
multiple charged ion.
negative ion.
metastable ion.
isotopes ion.
Introduction, Basic Principles, Terminology, Instrumentation, Ionization techniques (EI, CI, FAB, MALDI, and ESI), Mass Analyzer (Magnetic sector instruments, Quadrupole, TOF, and ICR ), and Applications of Mass Spectrometry.
Photoelectron spectroscopy
- a single photon in/ electron out process
• X-ray Photoelectron Spectroscopy (XPS)
- using soft x-ray (200-2000 eV) radiation to
examine core-levels.
• Ultraviolet Photoelectron Spectroscopy (UPS)
- using vacuum UV (10-45 eV) radiation to
examine valence levels.
This slide discusses the principle, instrumentation, process, detectors, sample ,solvents used in mass spectroscopy and also its applications and limitations.
MASS SPECTROSCOPY ( Molecular ion, Base peak, Isotopic abundance, Metastable ...Sachin Kale
CONTENT:
Molecular Ion Peak
Significance of Molecular ion & Graphically Method
Base Peak
Isotopic Abundance
Metastable Ion
Significance of Metastable ion
Nitrogen Rule & graphs
Formulation of Rule
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
TYPES OF PEAKS IN MASS SPECTROSCOPY.pptxAnupamaCp2
Types of peaks in mass spectroscopy.
Molecular ion or parent peak.
base peak.
fragment ions.
rearrangement ion.
multiple charged ion.
negative ion.
metastable ion.
isotopes ion.
Introduction, Basic Principles, Terminology, Instrumentation, Ionization techniques (EI, CI, FAB, MALDI, and ESI), Mass Analyzer (Magnetic sector instruments, Quadrupole, TOF, and ICR ), and Applications of Mass Spectrometry.
various parts of mAss spectroscopy, applications, principle, peaks, rules, typical mass spectra, various combinations, Fragmentation, rules of fragmentation and useful points which can help Chemical and analytical students and structural elucidation.
It is an analytical technique useful for the determination of molecular mass, molecular formula and fragmentation pattern of particular molecule and compounds. It has greater application in pharmaceutical and medicinal fields.
Mass spectroscopy is an analytical technique used to measure the mass-to-charge ratio (m/z) of one or more molecules present in a sample. It can be used to identify unknown compounds via molecular weight determination, quantify known compounds, and determine the structure and chemical properties of molecules.2 Mass spectroscopy is also useful for studies on protein-protein interactions. The basic principle involves fragmentation of a compound or molecule into charged species, which are accelerated, deflected, and finally focused on a detector according to their mass and charge ratio.Mass spectroscopy is an instrumental method for identifying the chemical constitution of a substance by means of the separation of gaseous ions according to their differing mass and charge.
Mass spectrometry principle working inttumentation advantages diadvantages GC...sneha010196
Mass spectroscopy
History
What is mass number
Ms principle
Ms working
Instrumentation
Mass analysers
Fragmentation rules
Nitrogen rule
Types of peaks
Interpretation of spectra
Gc ms
Applications of ms
Advantages & disadvantages
Reference.
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Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
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The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
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Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
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Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
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2. Mass Spectrometry
Technique that utilizes the degree of deflection of
charged particles by a magnetic field to find the relative
masses of molecular ions and fragments
a powerful method because it provides a great deal of
information and can be conducted on tiny samples
2
3. Applications
Determining molecular mass
Molecular formula (HRMS)
Confirmation of elemental composition
Finding out the structure of an unknown substance
Verifying the purity of a known substance
Providing data on isotopic abundance
3
4. Reaction monitoring
Crude reaction mixture
Stability studies
Quick product identification (TLC spot)
Selective detector for GC/HPLC
MS provides molecular weight information about
each chromatographic peak
4
5. • Resolution: A measure of how well a mass
spectrometer separates ions of different
mass.
– low resolution: Refers to instruments capable
of separating only ions that differ in nominal
mass; that is ions that differ by at least 1 or
more atomic mass units.
– high resolution: Refers to instruments
capable of separating ions that differ in mass
by as little as 0.0001 atomic mass unit.
Low and High Resolution MS
5
6. • A molecule with mass of 44 could be C3H8,
C2H4O, CO2, or CN2H4
• If a more exact mass is 44.029, pick the
correct structure from the table:
C3H8 C2H4O CO2 CN2H4
44.06260 44.02620 43.98983 44.03740
High Resolution MS
6
7. – C3H6O and C3H8O have nominal masses of 58 and
60, and can be distinguished by low-resolution MS.
– C3H8O and C2H4O2 both have nominal masses of 60.
– Distinguish between them by high-resolution MS.
C2 H4 O2
C3 H8 O
60.02112
60.05754
60
60
Molecular
Formula
Nominal
Mass
Precise
Mass
Resolution
– High resolution MS can replace elemental analysis for
chemical formula confirmation 7
8. Mass Spectrometry
Doesn’t involve the absorption of any type of light
Technique involves
Creating gas phase ions from the analyte atoms or
molecules
Separating the ions according to their mass-to-charge
ratio (m/z)
Measuring the abundance of the ions
8
we don’t get the sample back; a destructive method
9. Steps to Get Mass Spectrum
A compound is vaporized and ionized by
bombardment with a beam of high-energy electrons
The electron beam ionizes the molecule by causing
it to eject an electron.
When the electron beam ionizes the molecule, the
species formed is called a radical cation, symbolized as
M+•.
9
10. The radical cation M+• is also called the molecular ion
or parent ion; its mass = molecular weight of M
Because M+• is unstable it decomposes to form
fragments of radicals and cations that have a lower
molecular weight than M+•
The mass spectrometer measures the mass of these
cations.
10
11. The mass spectrum is a plot of the amount of each cation
(relative abundance) versus its mass to charge ratio (m/z)
Since z is almost always +1, m/z actually measures the mass
(m) of the individual ions
11
13. 13
The m/q ratio of the ions that reach the detector can be varied by changing either the
magnetic field (B) or the applied voltage of the ion optics (V)
14. By varying the voltage or magnetic field of the
magnetic-sector analyzer ,
the individual ion beams are separated spatially and
each has a unique radius of curvature according to its
mass/charge ratio.
14
16. EI: Electron Ionization/Electron Impact
Heated Incandescent
Tungsten/Rhenium Filament
Accel!
e
Vaporized
Molecules
70 eV
Ions To Mass
Analyzer
16
How EI works:
Electrons are emitted from a
filament made of tungsten
cathode
They are accelerated
towards anode by a
potential of 70 eV
The electrons and
molecules cross (usually at
a right angle) and collide
The ions are primarily
singly-charged, positive
ions, that are extracted by a
small potential through a slit
17. Referred to as hard ionization source due to the high
energy EI source
Ions are accelerated into the mass analyzer by an
accelerating voltage of ~ 104 V
Both negative and positive ions are formed by EI
Negative ions form from molecules containing acid
groups or electronegative atoms
17
18. Collision between ions and molecules may also result in ions
with higher m/z values than the molecular ion
An example is the (M+1) peak
Reaction between analyte molecule and H+ to form MH+ or
(M+H)+ in which charge equals a+1
Low pressure in the ionization source minimizes reaction
between ions and molecules
18
19. Advantages of Electron Ionization
• inexpensive, versatile and reproducible
• fragmentation gives structural information
• large databases if EI spectra exist and are
searchable
Disadvantages of Electron Ionization
• fragmentation at expense of molecular ion
• sample must be relatively volatile
19
20. Chemical Ionization (CI)
A large excess of reagent gas (1000 – 10000 times) is
introduced into the ionization region
Pressures in source are typically higher than EI
Electrons are allowed to bombard the gas-sample
mixture
Examples of reagent gas
- Methane, ammonia, isobutane
20
21. Reagent gases are much more likely ionized by the
electrons than the sample due to large excess
Sample molecules are subsequently ionized by
collision with ionized reagent gas molecules
Considered as soft ionization source
Less fragmentation and molecular ion is much more
abundant
Combination of CI and EI spectra provide good
interpretation
21
CI cont’d
22. Chemical Ionization (CI)
- For methane as reagent gas
electrons
with
n
interactio
upon
formed
are
CH
and
CH 3
4
3
5
4
4 CH
CH
CH
CH
2
5
2
4
3 H
H
C
CH
CH
Proton transfer occurs when sample molecules collide with
5
2
5 H
C
and
CH
22
23. Chemical Ionization (CI)
4
5 CH
MH
CH
M
The following may occur if analyte is a saturated HC
4
2
5
2 H
C
MH
H
C
M
2
4
5 H
CH
H)
-
(M
CH
M
6
2
5
2 H
C
H)
-
(M
H
C
M
29)
(M
m/z
with
)
H
C
(M
H
C
M 5
2
5
2
23
25. Fast Atom Bombardment (FAB)
material to be analyzed is mixed with a non-volatile
chemical protection environment called a matrix
This is bombarded under vacuum with a high energy (4
– 10 keV) beam of atoms, which forms ions
atoms are typically an inert gas (Ar or Xe)
common matrix include glycerol, thioglycerol, 3-
nitrobenzyl alcohol, 18-Crown-6 ether, 2-
nitrophenyloctyl ether, sulfolane, diethanolamine, and
triethanolamine.
25
29. MALDI
Combined with a MS detector, MALDI became
an indispensable tool in analysis of
biomolecules and organic macromolecules
MALDI involves
incorporation of the analyte into a matrix,
absorption/desorption of laser radiation, and
then ionization of the analyte
29
30. Formation of Matrix-Analyte
The analyte incorporation in to a suitable matrix is
the first step of the MALDI process, and is an
important feature of the MALDI method
A typical sample preparation involves using 10-6 M
solution of the analyte mixed with 0.1 M solution of
the matrix.
The solvents are then evaporated in a vacuum of the
MS, and the matrix crystallizes with the analyte
incorporated.
30
31. MALDI Matrix
The matrix must meet the following properties and
requirements:
Be able to embed and isolate analytes (e.g. by co-
crystallization)
Be soluble in solvents compatible with analyte
Be vacuum stable
Absorb the laser wavelength
Cause co-desorption of the analyte upon laser
irradiation
Promote analyte ionization
31
33. MALDI
• The mechanism remains
uncertain
• It may involve absorption
of light by the matrix
• Transfer of this energy to
the analyte
– which then ionizes
into the gas phase as a
result of the relatively
large amount of
energy absorbed.
– To accelerate the
resulting ions into a
flight-tube in the mass
spectrometer they are
subjected to a high
electrical field.
33
34. produce gaseous ionized molecules from a liquid
solution by creating a fine spray of droplets in the
presence of a strong electric field
one of the most important techniques for analyzing
biomolecules, such as polypeptides, proteins having
MW of 100,000 Da or more
Generates positive (M+nH)n
+ and negative (M - nH)n
-
ions and almost no fragmentation. Generates multiple
charged ions.
Easily coupled to HPLC
Electrospray ionization (ESI)
34
36. The Nature of Mass Spectra
• Molecular ion - The ion obtained by the loss of one electron
from the molecule (M+)
• Base peak - The most intense peak in the MS, assigned 100%
intensity
• Fragment ions - Lighter cations formed by the decomposition
of the molecular ion. These often correspond to stable
carbcations.
36
38. Isotopes
Mass spectrometers are capable of separating and detecting individual
ions even those that only differ by a single atomic mass unit
As a result molecules containing different isotopes can be
distinguished
This is most apparent when atoms such as bromine or chlorine are present
(79Br : 81Br, intensity 1:1 and 35Cl : 37Cl, intensity 3:1) where peaks at "M"
and "M+2" are obtained
The intensity ratios in the isotope patterns are due to the natural
abundance of the isotopes
"M+1" peaks are seen due to the presence of 13C in the sample.
38
42. Getting the Formula from the Mass Spectrum
The Nitrogen Rule
if m/z for M is odd, then the molecular formula must have an odd
number of nitrogens.
If m/z for M is even, then the molecular formula must have an
even number of nitrogens (this includes 0).
For 1-bromopropane, m/z for M=122. The even number is in
accordance with the even number of nitrogens in the formula
(zero).
42
43. 43
The Hydrogen Rule
the maximum number of hydrogens in the molecular formula
is 2C+N+2.
C = # of carbons,
N = # of nitrogens
EX: For CH3CH2CH2Br, there are three carbons, so the max #
of hydrogens is 2(3)+2=8
44. The “Rule of 13” as an aid to guessing a molecular
Formula
Take the Weight of ion, divide by 13
This answer is N, for (CH)N and any numerical remainder is added as H
e.g.; 92. when 92/13 = 7 with remainder = 1; C7H8 weighs 92. This is our
candidate formula.
Can evaluate other alternative candidate formulas possessing heteroatoms.
For each member of the list below, replace the indicated number of CHs in the
above answer
Hetero
substitution
CH
replacement
Hetero
substitution
CH replacement
O CH4 P C2H7
N CH2 S C2H8
O+N C2H6 O+S C4
F CH7 I C10H7
Si C2H4 Cl, Br (use isotopes) 44
45. Degree of unsaturation (One Double Bond Equivalent) is one pi bond or one ring
A triple bond counts as 2 DBE
Having 4 DBE indicates the possibility of a benzene ring. The formula for DBE is
the following:
Important Note: DBE can never be negative and fractional
EX: For CH3CH2CH2Br, the DBE equals 3-(8/2)+(0/2)+1=0. (No pi bonds, no rings.)
45
47. Molecular ion
Three facts:
The peak must correspond to the highest mass ion on
the spectrum excluding the isotopic peaks
The ion must have an odd number of electrons –
usually a radical cation
The ion must be able to form the other fragments on
the spectrum by loss of logical neutral fragments
47
48. Fragmentation
The time between ionization and detection in most mass
spectrometer is 10-5 sec.
– If a particular ionized molecule can “hold together”
for greater than 10-5 sec. a M+ ion is observed
– If a particular ionized molecule fragments in less
than this time, the fragments will be observed
48
49. Fragmentation – Chemistry of Ions
• One bond s-cleavages:
a. cleavage of C-C
b. cleavage of C-heteroatom (i-cleavage)
C C C C
+
C Z C Z
+
49
50. Fragmentation – Chemistry of Ions
• One bond s-cleavages: a-cleavage of C-heteroatom
C C Z C C Z
+
C C Z C C Z
+
C C Z C Z
+ C
50
51. Fragmentation – Chemistry of Ions
Two bond s-cleavages/rearrangements:
a. Elimination of a vicinal H and heteroatom:
b. Retro-Diels-Alder
C C Z Z
+ H
H
C C
+
51
53. Fragmentation – Chemistry of Ions
When deducing any fragmentation scheme:
The even-odd electron rule applies: “thermodynamics dictates
that even electron ions cannot cleave to a pair of odd electron
fragments”
The order of carbocation/radical stability is benzyl/3° >
allyl/2° > 1° > methyl > H * the loss of the longest carbon
chain is preferred
Fragment ion stability is more important than fragment radical
stability
53
54. Fragmentation Patterns of Groups
Alkanes
Apply the stability of carbocations (or radicals)
This is governed by Stevenson’s Rule – the fragment with the
lowest ionization energy will take on the + charge – the other
fragment will still have an unpaired electron
Example: iso-butane
CH3
+
CH3
+
54
55. For straight chain alkanes, a M+ is often observed
Ions observed: clusters of peaks CnH2n+1 apart from the loss of –
CH3, -C2H5, -C3H7, etc.
Fragments lost: ·CH3, ·C2H5, ·C3H7, etc.
In longer chains – peaks at 43 and 57 are the most common
55
58. Alkenes
Ions observed: clusters of peaks CnH2n-1 apart from -C3H5, -C4H7, -
C5H9 etc. at 41, 55, 69, etc.
Terminal alkenes readily form the allyl carbocation, m/z 41
R
H2
C
+
R
C
H
CH2 H2C C
H
CH2
58
61. Mass spectrum of 1-methyl-1-cyclohexene
Side chains are easily fragmented
M+ 96
81
68
61
62. Alkynes – Fragment Ions
For terminal alkynes, the loss of terminal hydrogen is observed
(M-1)
Terminal alkynes form the propargyl cation, m/z 39 (lower
intensity than the allyl cation)
R
H2
C
+
R
C CH H2C C CH
62
64. Aromatic Hydrocarbons – Fragment Ions
Very intense molecular ion peaks and little fragmentation of the ring
system are observed
Where alkyl groups are attached to the ring, a favorable mode of
cleavage is to lose a H-radical to form the C7H7
+ ion (m/z 91)
This ion is believed to be the tropylium ion; formed from
rearrangement of the benzyl cation
CH2
CH3
75 eV e-
64
68. McLafferty Rearrangements in Alkyl Benzenes
a
CH2
CH2
CHCH3
H
m+ 134
loss of CH
3CH=CH2
.
+
CH2
H
H
+
.
m/e 92
CH2
+
m/e 91
- propyl
.
68
69. Alcohols– Fragment Ions
The largest alkyl group is usually lost; the mode of cleavage typically
is similar for all alcohols:
primary
secondary
tertiary
OH
H2C
O H
+
O H
+
OH O H
+
OH
m/z
31
59
45
69
71. Alcohols– Fragment Ions
Dehydration (M - 18) is a common mode of fragmentation
For longer chain alcohols, a McLafferty type rearrangement can
produce water and ethylene (M - 18, M - 28)
O
H
R H O
H
R
H
+
71
72. Ethers– Fragment Ions
The largest alkyl group is usually lost to a-cleavage; the
mode of cleavage typically is similar to alcohols:
Cleavage of the C-O bond to give carbocations is
observed where favorable
R
H2
C O R R H2C O R
+
R
H
C O R R CH O R
R
R
+
72
73. Ethers– Fragment Ions
Aromatic ethers can generate the C6H5O+ ion by loss of
the alkyl group rather than H; this can expel CO as in
the phenolic degradation
O
R
O
R + C O + C5H5
+
73
76. Aldehydes - Fragment Ions
a-cleavage is characteristic and often diagnostic for aldehydes – can
occur on either side of the carbonyl
-cleavage is an additional mode of fragmentation
R H
O
R C O + H
R H
O
H C O
+
R
M-1 peak
m/z 29
H
O
+
R
R
H
O m/z R+
M - 41
can be R-subs.
76
77. Aldehydes - Fragment Ions
d) McLafferty rearrangement is observed if -Hs present
e) Aromatic aldehydes – α-cleavages are more favorable, both to lose H· (M -
1) and HCO· (M – 29)
m/z 44
m/z R+
Remember:
aromatic ring
can be subs.
+
O
H
H
R
O
H
R
C O + H
H
O
O
H
+
O
H
77
78. Ketones - Fragment Ions
a-cleavage can occur on either side of the carbonyl – the
larger alkyl group is lost more often
R R1
O
R C O + R1
R1 is larger than R
M – 15, 29, 43…
m/z 43, 58, 72, etc.
78
79. Ketones - Fragment Ions
McLafferty rearrangement is observed if -H’s present
Aromatic ketones – a-cleavages are favorable primarily to lose R· (M – 15,
29…) to form the C6H5CO+ ion, which can lose CO
Remember:
aromatic ring
can be subs.
+
O
R1
H
R
O
H
R
R1
C O + R
O
R
+ C O
m/z 105
m/z 77 79
81. Esters
Most important a-cleavage reactions involve loss of the
alkoxy- radical to leave the acylium ion
The other a-cleavage (most common with methyl esters,
m/z 59) involves the loss of the alkyl group
R
R1
O
R C O + OR1
O
R
R1
O
R C
O
+
O
O R1
81
82. Esters - Fragment Ions
McLafferty occurs with sufficiently long esters
R1
O
+
O
H
R1
O
O
H
82
83. Esters - Fragment Ions
One interesting fragmentation is shared by both benzyloxy esters and
aromatic esters that have an ortho-alkyl group
O
O
H
OH
fragmentation
+
CH2
C
O
ketene
O
R
O
C
H2
H
C
HO
R
O
CH2
+
benzyloxy ester
ortho-alkylbenzoate ester
83
84. Esters (benzoic) – methyl ortho-toluate
M+ 150
C
O
CH2
O
O
119
O
O
91
m/z 118
84
85. Carboxylic Acids - Fragment Ions
Most important a-cleavage reactions involve loss of the hydroxy-
radical to leave the acylium ion
The other a-cleavage (less common) involves the loss of the alkyl
radical. Although less common, the m/z 45 peak is somewhat
diagnostic for acids.
R
H
O
R C O + OH
O
R
H
O
R C
O
+
O
O H
85
86. Carboxylic Acids - Fragment Ions
McLafferty occurs with sufficiently long acids
aromatic acids degrade by a process similar to esters, loss of the HO·
gives the acylium ion which can lose CO:
H
O
+
O
H
H
O
O
H
m/z 60
H
O
H
O
C
O
+
+ further loss of
CO to m/z 77
86
87. Carboxylic Acids - Fragment Ions
As with esters, those benzoic acids with an ortho-alkyl group will lose
water to give a ketene radical cation
O
H
O
C
H2
H
C
HO
H
O
CH2
+
ortho-alkylbenzoic acid
87
90. Amines
Follow nitrogen rule – odd M+, odd # of nitrogens
a-cleavage reactions are the most important
fragmentations for amines; for 1° n-aliphatic amines
m/z 30 is diagnostic
R
C
N R
C
N
+
90
92. Amides
Follow nitrogen rule – odd M+, odd # of nitrogens;
a-cleavage reactions afford a specific fragment of m/z
44 for primary amides
McLafferty observed where -hydrogens are present
R
C
NH2
O
R + O C NH2
m/z 44
O
NH2
H
O
NH2
H
+
92
93. Example MS: amides (aromatic) – benzamide
M+ 121
C
NH2
O
77
C
NH2
O
105
93
95. Nitriles - Fragment Ions
Follow nitrogen rule – odd M+, odd # of nitrogens
Principle degradation is the loss of an H-atom (M – 1) from a-
carbon:
Loss of HCN observed (M – 27)
McLafferty observed where -hydrogens are present
H +
R
H2
C C N R C
H
C N
C
N
H
H2C
C
N
H
+
m/z 41
95
97. Halogens - Fragment Ions
Fluoro- and iodo-compounds do not have appreciable
contribution from isotopes
Chloro- and bromo-compounds are unique in that they will show
strong M+2 peaks for the contribution of higher isotopes
For chlorinated compounds, the ratio of M+ to M+2 is about 3:1
For brominated compounds, the ratio of M+ to M+2 is 1:1
97
98. Halogens - Fragment Ions
Principle fragmentation mode is to lose halogen atom,
leaving a carbocation
Loss of HX is the second most common mode of
fragmentation
R + X
R X
R +
C X
C
R
H
H H
H
C
H
CH2 H X
98
104. Gas Chromatography-Mass Spectrometry (GC-MS)
To analyze a urine sample for tetrahydrocannabinol, (THC) the principle
psychoactive component of marijuana, the organic compounds are extracted from
urine, purified, concentrated and injected into the GC-MS
THC appears as a GC peak, and gives a molecular ion at 314, its molecular weight
104
105. Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS, involves multiple
steps of mass spectrometry selection, with some form of fragmentation
occurring in between the stages.
105