2. What is biochemistry
• Biochemistry studies the chemical reactions that
occurs in living organisms
• In general it deals with body substance like
enzymes, hormones, carbohydrates, amino acids,
fats, proteins, DNA, RNA etc.
• Biochemists study their origin, formation,
functions and deficiency diseases.
3. Importance of biochemistry in
PHARMACY and NURSING
Biochemistry will help you to better understand
Drug Constitution
The half life of drugs
Drug storage
Drug metabolism
Biochemical tests
5. A molecule/ Monomer?
•A group of two or more atoms
held together by covalent bonds.
E.g. H2O, Glucose,
A macromolecule/Polymer?
A long molecule made of
monomers bonded
together to form a polymer
Molecule vrs Macromolecules
7. Formed from Condensation reactions called
dehydration synthesis (removal of water)
Forming Macromolecules
8. • Hydrolysis reaction
– Hydro = water;
– lysis = break
– Water is added and the lysis of the polymer occurs.
Forming Monomers
9. Monomer/ molecule -----
Polymer/ macromolecule----
Polymers are formed from—
Monomers are formed form---
The four types main types macromolecules are ........
Atoms joined by covalent
bonds
Long unit made of monomer
units
Condensation / dehydration
Hydrolysis or rehydration
Memory Check
13. CARBOHYDRATES
Carbohydrates are the most
abundant organic molecules
in nature.
Carbohydrates are present in
humans, animal tissues, plants and
in micro-organisms.
Carbohydrates are also present in
tissue fluids, blood, milk,
secretions and excretions of
animals
14. CARBOHYDRATES
The term carbohydrate
means a hydrate of carbon
Its general formular is
Cn(H2O)n
Carbohydrates are defined as
organic substances containing
C, H, O
Where H and O are in the ratio 2:1 as
found in H2O
15. CARBOHYDRATES
MONOSACCHARIDES OLIGOSACCHARIDES POLYSACCHARIDES
Classification Of Carbohydrate
Cannot be hydrolysed
into smaller unit
Two or more monomer units
On hydrolysis yield
monosaccharides or
oligosaccharides
a. Classified based on
the no. of C-atoms
a.Disaccharides
b.Trisachharides
c.Tetrasachharides
Basic units of
carbohydrates
They can be further
hydrolysed to smaller units
b.Classified based on the
type of functional group
a.Homo-polysaccharides
b.Hetro-polysaccharides
16. Based on the no of C-atoms Based on the functional group
- Trioses (C3H6O3)
- Tetroses (C4H8O4)
- Pentoses (C5H10O5)
- Hexoses (C6H12O6)
- Heptoses (C7H14O7)
- Aldoses :
- Aldehyde –CHO
e.g. Glyceraldehyde
Glucose
- Ketoses :
- ketone ( C = O)
e.g.Dihydroxyacetone
,
Classification Of Monosaccharides
MonosaccharidesMonosaccharides
21. Uses of Monosaccharides
• Glucose ( 50%) is hypertonic and
provides a source of calories in a
minimal volume of water.
• Glucose 50% is frequently used to
restore blood glucose concentrations
in the treatment of hypoglycaemia
resulting from insulin excess or from
other causes.
22. • Fructose: as an
intravenous energy source
for patients with:
• Hepatic disease,
• Uncontrolled diabetes
mellitus
• Postoperative state.
Uses of Monosaccharides
23. Uses of Monosaccharides
• Dextrose is the name of a simple sugar chemically
identical to glucose (blood sugar) that is made
from corn.
• Dextrose is dissolved in solutions that are given
intravenously used to increase a person’s blood
sugar, which can be combined with other drugs, or
.
• Dextrose is also available as an oral gel or tablet.
Because dextrose is a “simple” sugar, the body can
quickly use it for energy.
25. OLIGOSACCHARIDES
They are formed by condensation of 2-9 monosaccharides
they are
a.Disaccharides (sucrose, lactose)
b.Trisaccharides (raffinose)
c.Tetrasaccharides (stachyose)
The smallest and the commonest oligosaccharides are
Disaccharides
26. Disaccharides
A disaccharide consists of 2 monosaccharide units (similar or
dissimilar)held together by a glycosidic bond( links a sugar to
another group)
They are crysatalline, water soluble and sweet to taste.
27. OOHOH
C C
C
C O
C
H
OH
OHOH
OH
H
H
CH2OH
H
H
CHCH22OHOH
C C
C
C O
C
H
OH
OHOH
OH
H
H
CH2OH
H
H
C C
C
C O
C
H
OH
OH
OH
H
H
CH2OH
H
H
C C
C
C O
C
H
OH
OH
OH
H
H
CH2OH
H
H
O
H H
Glycosidic Bond
This is a CONDENSATION reaction, where a water molecule is lost.
Glucose
Maltose
Are joined by a
glycosidic linkage
(Dehydration/ condensation reaction)
Forming Disaccharides
29. Uses of Disaccharides
• Maltose can be converted from icodextrin
which is used in dialysis solutions.
• Sucrose : It is often used in medications
to impart a more pleasant taste to often
unpalatable chemicals
• Iron Sucrose : is a medicine which is used
in iron deficiency.
30. - Made up of repeating units of monsaccharides held by
glycosidic bonds
Polysaccharides (Glycans)
- During its formation a water molecule is released at each
condensation
-Unlike sugars they are not sweet.
31. - HOMOGLYCANS
- -Made up of only 1 type of
monosaccharide monomers
- Starch
- Glycogen
- Dextran
HETEROGLYCANS
-Made up condensation of2 or
more types of monosaccharides
• agar,
• Chitin,
• peptidoglycans etc
- They are ideal as STORAGE AND AS STRUCTURAL
COMPONENTS
Polysaccharides (Glycans)
- They are of 2 types Homoglycans and Heteroglycans.
32. • DextranDextran :: complex branched
glucan
• Polysaccharide made of
many glucose
• It is used medicinally as anIt is used medicinally as an
antithrombotic, to reduceantithrombotic, to reduce
blood viscosityblood viscosity
Polysaccharides (Glycans)
33. Polysaccharides (Glycans)
Glycogen:
Storage of excess
sugar in animals;
stored in liver and
muscles
It is the animal
short-term storage
form of energy
35. Starch or amylum: consists
of a large number of
glucose units joined by
glycosidic bonds.
This polysaccharide is
produced by most green
plants as an energy store
Polysaccharides (Glycans)
37. InulinInulin
Polymer of fructose i.e. fructosan
Found in bulbs, garlic, onion etc
Inulin is not readily metabolised in the
human body and is readily filtered
through the kidney. Hence used for
testing kidney function
Polysaccharides (Glycans)
38. STORAGE form of energy (starch and glycogen).
Main SOURCE of energy in the body( glycogen).
Excess carbohydrate is converted to fat.
General Functions carbohydrates
39. Structural basis of many organisms
1.Cellulose of plants
2. Exoskeleton of insects
3. Cell wall of microorganisms
General Functions carbohydrates
40. Components of several animal structure and
plant structures
1.cartilage
2.tendons,
General Functions carbohydrates
42. Ascorbic acid, a derivative of carbohydrate
is a water-soluble vitamin
General Functions carbohydrates
43. Derivatives of carbohydrates are components of
antibiotics like erythromycin
General Functions carbohydrates
used in the treatment of infections caused by Gram-
positive bacteria. It is similar in its effects to penicillin.
45. dr.aarif
With the water, I say, Touch me not,
To the tongue, I am tasteful,
Within limits, I am dutiful,
In excess, I am dangerous
LIPIDS
46. dr.aarif
Lipids are the chief concentrated storage form of energy forming
about 3.5% of the cell content.
Lipids are organic substances relatively
insoluble in water
soluble in organic solvents (alcohol, ether)
Lipids
47. Lipids consistLipids consist mostly of hydrocarbons (C , H, and O)(C , H, and O)
Lipids
Hydrophilic Head (“hydro”=water; “philic” = loving,
Hydrophobic Tails ( Phobic- fearing)
Polar Head
(hydrophilic)Glycerol
Fattyacid
Fattyacid
Non-Polar Tail
(Hydrophobic)
49. SIMPLE LIPIDS
They are esters of fatty acids with alcohol. They are of 2 types :
1. Neutral or true fats : Esters of fatty acids with glycerol
2. Waxes : Esters of fatty acids with alcohol other than glycerol.
Neutral / True fats/Triacylglycerol
True fats are made up of C, H, & O but O is less
A fat molecule is made up of 2 components :
a)GLYCEROL
b) FATTY ACIDS (1-3 mol, of same or diff long chained)
50. Glycerol: A glycerol mol has 3 carbons
each bearing a –OH group
A fatty acid mol is an unbranched
chain of C-atoms.
It has a –COOH group at one end and a
H bonded to almost all the C-atoms
Fatty acids may be saturated or
unsaturated
SIMPLE LIPIDS
1.Neutral / True fats
51. When a fatty acid attaches to glycerol
it becomes a fat
Fats have a structure that looks like a E
Unsaturated fatty acid in a fat makes it
an unsaturated fat
Saturated fatty acid in a fat makes it a
saturated fat
SIMPLE LIPIDS
1.Neutral / True fats/Triacylglycerol
52. Unsaturated fats :
– one or more double bonds in the
fatty acids allows for “kinks” in the
tails
– liquid at room temp
– most plant fats
(a) Saturated fat and fatty acid
Stearic acid
(b) Unsaturated fat and fatty acid
cis double bond
causes bending
Oleic acid
Saturated fats:
No double bonds in fatty
acid tails
solid at room temp
most animal fats
SIMPLE LIPIDS
1.Neutral / True fats
53. Functions Simple Lipids
1.Neutral / True fats
1. Storage form of energy
(triacylglycerol) in adipose
tissues
• Adipose tissue is the major
storage site for fat in the
form of triglycerides in the
skin
• Fat under skin serve as
thermal insulator against
cold.
54. Functions Simple Lipids
1.Neutral / True fats
1. Fat around kidney and
heart serve as padding
against injury.
2. Act as electric insulators
in neurons as myelin
sheath
3. Help in absorption of fat
soluble vitamins (A, D, E
and K)
55. 2. WAXES
Lipids which are long chain saturated fatty acids and a long chain
Saturated alcohol of high mol wt other than glycerol
Example :
1.Bees wax : secretion of abdominal glands of worker honey bees
2.Shellac wax - from the lac insect kerria lacca
3.Lanolin or wool fat : Secretion of cutaneous glands and obtained
from the wool of sheep
3. Sebum : secretion of sebaceous glands of skin
4. Cerumen : soft and brownish waxy secretion of the glands in the
SIMPLE LIPIDS
57. Uses Waxes
SIMPLE LIPIDS
Lanolin is frequently used in
protective baby skin treatment and
as a treatment for sore nipples in
breastfeeding mothers.[13]
Shellac wax is used in dental
technology, in (partial) denture
production.
58. COMPLEX LIPIDS
They are derivatives of simple lipids having
additional group like
phosphate,
N2-base,
Protein etc.
They are further divided into
Phospholipids,
Glycolipids,
Lipoproteins.
Polar Head
(hydrophilic)
Phosphate
Protein
Glycerol
Fattyacid
Fattyacid
N-Base
Non-Polar Tail
(Hydrophobic)
65. 3. Lipoprotein
5 types of lipoproteins :
1. chylomicrons
2. VLDL (Very Low density Lipoprotein)
3. LDL (Low density Lipoprotein)
4. HDL (High density Lipoprotein)
5. Free fatty acid albumin complex
COMPLEX LIPIDS
They contain lipids and proteins in their molecules.
They are main constituent of membranes.
They are found in milk and Egg yolk.
67. DERIVED LIPIDS
They are derivatives obtained on the hydrolysis of the simple
and complex lipids.e.g. steroids, terpenes. etc
1. Steroid
The steroids do not contain
fatty acids but are included in
lipids as they have fat-like
properties.
They are made up of 4
fused carbon rings
68. DERIVED LIPIDS
Functions of Steroids
• Cholesterol
Hormone production. Cholesterol
plays a part in producing hormones
such as estrogen, testosterone,
progesterone, aldosterone and
cortisone
Vitamin D production. Vitamin D is
produced when the sun’s ultraviolet
rays reach the human skin surface.
69. DERIVED LIPIDS
Functions of Steroids
• Cholesterol
Cell membrane support.
Cholesterol plays a very
important part in both the
creation and maintenance of
human cell membrene
70. Functions of Terpenes
Terpenes are a major component of essential oils produced by
plants. They give fragrance and perform various therapeutic
functions
DERIVED LIPIDS
71. Diseases associated with abnormal chemistry or
metabolism of lipids-
71
Clinical significance of lipids
Obesity
Atherosclerosis
Diabetes Mellitus
Hyperlipoproteinemia
Fatty liver
Lipid storage diseases
72. 72
Clinical significance of lipids
1.Excessive fat deposits cause obesity. obesity is a risk factor for
heart attack.
2. Abnormality in cholesterol and lipoprotein metabolism leads to
atherosclerosis and cardiovascular diseases .
3. In diabetes mellitus, the metabolisms of fatty acids and
lipoproteins are deranged, leading to ketosis
73. 73
Clinical significance of lipids
4. Hyperlipidemia, hyperlipoproteinemia, or hyperlipidaemia
involves abnormally elevated levels of any or all lipids and/or
lipoproteins in the blood . It is the most common form of
dyslipedemia (which includes any abnormal lipid levels).
5. Some fat in your liver is normal. But if it makes up more than 5%-
10% of the organ's weight, you may have fatty liver disease.
6. Lipid storage diseases are a group of inherited metabolic
disorders in which harmful amounts of fatty materials (lipids)
accumulate in various tissues and cells in the body
74. Discussion Question
1. How will my knowledge about carbohydrate
and lipids structure and function enrich my
work as a health professional.(outline 5points)
2. What are the uses of carbohydrates and lipids
in medicine .(5 uses)
79. General Functions of Proteins
1. Transport of substances in the body . E.g. Haemoglobin
transports oxygen.
2. Enzymes which catalyze chemical reactions in the body
3. Defence function .e.g Immunoglobulins(antibodies)
4. Hormones are proteins. They control many biochemical
events. Example: Insulin.
80. 5. Contraction of muscles. E.g Muscle proteins(actin and
myosin).
6. Gene expression. They control gene expression and
translation.E.g Histones.
7. Nutrient and storage .E.g albumen of egg, Casein of
milk , Ferritin that stores iron.
Functions of Protiens
81. 8. Proteins act as buffers. E.g Plasma proteins.
9. Proteins function as anti-vitamins. E.g. Avidin
of egg.
10.Proteins are infective agents.e.g Prions
which cause mad cow disease are proteins.
Medical And Biological Functions
82. 10. Some toxins are proteins E.g Enterotoxin of
cholera microorganism.
11. Some proteins provide structural strength and
elasticity to the organs and vascular system. E.g
Collagen and elastin of bone matrix and ligaments.
10. Some proteins are components of structures of
tissues. E.g α-keratin is present in hair and
epidermis
Medical And Biological Functions
86. Building the nucleic acid polymer
Nucleotides are joined together by Phosphodiester bondsNucleotides are joined together by Phosphodiester bonds
ester bondester bond
ester bondester bond
Phosphodiester bondPhosphodiester bond
87. 1. DNA (Deoxyribonucleic acid)
– double stranded (DNA helix)
– can self replicate
– makes up genes which code for proteins is
passed from one generation to another
1. RNA (Ribonucleic acid)
– single stranded
– functions in actual synthesis of proteins coded
for by DNA
– is made from the DNA template molecule
Nucleic Acids Types
88. 1. They are involved in the storage, transfer and expression
of genetic information.
2. Some nucleic acids acts as enzymes and coenzymes.
E.g. RNA, act as catalyst and RNA is coenzyme for
telomerase which seals ends of chromosomes.
3. DNA exhibits structural polymorphism. It assumes
several forms depending on certain conditions. Several
DNA variants are known.
Functions of nucleic acid in the cell