This is a presentation created by me on"LASER SPECTROMETRY FOR MULTI-ELEMENTAL IMAGING OF BIOLOGICAL TISSUES"

1. LASER SPECTROMETRY FOR MULTI-
ELEMENTAL IMAGING OF BIOLOGICAL
TISSUES
RITOBRATA SENGUPTA
B.Sc. BIOTECHNOLOGY
SCHOOL OF LIFE SCIENCE
MANIPAL UNIVERSITY

2.  Journal:Scientific Reports
 Date of Publishing:14 August,2014
 Publisher:Nature Group of Publishers
 Volume:4 Article number:6065
 Impact Factor:5.228

3. REFERENCE OF THE MAIN PAPER:
Sancey,L.,Motto-
Ros,V.,Busser,B.,Kots,S.,Benoit,J.M.,Piednoir,A.,Lux,F.,Tillement,O.,
Panczer,G.,&Yu.J.Laser spectrometry for multi-elemental imaging of biological
tissues.Sci.Rep.4:6065;doi:10.1038/srep06065(2014).

4. ABOUT THE AUTHORS
 Institut Lumière Matière, UMR5306 Université Lyon
1-CNRS, Université de Lyon 69622 Villeurbanne
cedex, France

6. OVERVIEW
 This presentation contains discussions on:
 (i)LIBS :Definition & Concept on Quantum Physics
 (ii)Quantum Dot[QD]
 (iii)LASER
 (iv)Kidney
 (v)Detailed discussuion on LIBS(physical aspect
only,discussion on software not included in the research
article)
 (vi)Case study of Pearson’s Correlation Coefficient
 Advantages & Disadvantage of LIBS
 Inspirational Physicists & their explanation wrt Biological
aspect

7. LIBS[LASER INDUCED BREAKDOWN
SPECTROSCOPY]
 WHAT IS IT?
 LASER INDUCED BREAKDOWN
SPECTROSCOPY
 Used in imaging an component of a structure
 by using laser beam of a suitable wavelength
  based on the principle of ablasion of the
structure(here kidney) by pulsed laser beam.

8. PLASMA

9. AN INTRODUCTION TO PLASMA
PHYSICS!

10. AN ANALOGY:
Spectrum obtained
by Joseph von
Fraunhofer using his
spectrometer.

11. QUANTUM DOT{QD}
 Definition:A semiconductor crystal of nanometre
dimensions with distinctive conductive properties
determined by its size.


12. COLOUR VARIATION IN QDS

13. COMPARISON:ENERGY BANDS

14. LASER:LIGHT AMPLIFICATION BY
STIMULATED EMISSION OF RADIATION

15. ARCHITECHTURE OF KIDNEY

16. METHODS & PROCEDURE

17. METHODS & PROCEDURE

18. METHODS & PROCEDURE
r=Pearson’s
correlation
fator

19. COMPARISON:

20. COMPARISON:CONTD.
Molecular
dynamic
simuulation

21. OSERVATION & RESULTS
BLACK=LIBS SNR measured in epoxy resin
GREEN=Crater sizes measurd via SEM as a function of laser pulse
energy of the given range
(1)

22. BLUE:LIBS signal intensity(arbitrary unita.u.)
GREEN:The crater sizes measured via SEM as function of the
laser focus distance.
(2)

23. (3)

24. dGd & Na distribution in the cross-section of kidney
eMagnified view;
concentration of Gd & Na expressed in mM/L
white arrowsindicate the regions lacking in tissues,corresponding to blood
vessels & collecting ducts.
(4)

25. (5)
Quantitative imaging of
Gd,Si,Fe & Na in a
kidney cross-section
4hrs after the
nanoparticle
administration

26. TRYING TO UNDERSTAND THESE
GRAPHS: (OBS NO:6)

27. CASE STUDY:BASICS OF CORRELATION
COEFFICIENT
 Definition:A correlation coefficient is a number that
quantifies some type of correlation &
dependence,meaning statistical relationship
between two/more random variables/random
data values.
KARL PEARSON

28. CALCULATION:PEARSON’S
CORRELATION COEFFICIENT
SUBJECT AGE(x) GLUCOSE
LEVEL(y)
xy x^2 y^2
1 43 99 4257 1849 9801
2 21 65 1365 441 4223
3 25 79 1975 625 6241
4 42 75 3150 1764 5625
5 57 87 4959 3249 7569
6 59 81 4779 3481 6561
247 486 20485 11409 40022
CALCULATE TO FIND r

29. Dotted blue lines=
theoritical correlation
Red lines=measured
correlation
(6)contd.

30. (7)

34. DISADVANTAGE
 Major drawback is its undeniably invasive
naturethe tissue area subjected to test is to be
ablatedby pulsed laser beam.

35. PROF.BRIAN EDWARD COX

37. QUOTES OF PROF.BRIAN COX:WE ARE
STARDUST!

39. REFERENCES:
 Main paper:LASER SPECTROMETRY FOR MULTI-ELEMENTAL IMAGING OF
BIOLOGICAL TISSUES
 https://www.google.co.in/search?q=12+particles+of+matter+and+4+forces+of+nature&rlz
=1C1GIGM_enIN608IN608&site=webhp&source=lnms&tbm=isch&sa=X&ved=0ahUKEwi
Zjrf706rOAhWCpo8KHfyJD3YQ_AUICCgB&biw=1366&bih=599#imgdii=EWWPwWS1d5
yMjM%3A%3BEWWPwWS1d5yMjM%3A%3BcsoF9f9xlJwd0M%3A&imgrc=EWWPwWS
1d5yMjM%3A
 http://www.bbc.com/earth/story/20160128-how-do-we-know-what-stars-like-our-sun-are-
made-of

40. THANK
YOU!