Leishmania donovani is an intracellular protozoan parasite that causes visceral leishmaniasis (kala-azar). It was discovered in 1900 in India by William Leishman and Charles Donovan. It has two stages in its life cycle - the amastigote form found within macrophages in humans and other mammals, and the promastigote form found in the gut of sand flies. Sand flies transmit the parasite between hosts. In humans, the parasite infects macrophages in the liver, spleen and bone marrow, multiplying and causing enlargement of these organs. If left untreated, kala-azar is fatal. Current treatments include liposomal amphotericin B, miltefosine and par
Leishmania is a protozoan parasite that causes leishmaniasis, which exists in three main clinical forms: visceral leishmaniasis, cutaneous leishmaniasis, and mucocutaneous leishmaniasis. It is transmitted by the bite of infected female phlebotomine sand flies. Visceral leishmaniasis, the most severe form, affects the internal organs and is fatal if left untreated. Cutaneous leishmaniasis causes skin sores, while mucocutaneous leishmaniasis additionally affects the mucous membranes of the mouth and throat. Leishmania has a complex life cycle alternating between the sand fly vector and mammalian hosts.
Fasciolopsis buski is a large intestinal fluke that infects humans and pigs. It lives in the small intestine of its hosts and can measure up to 80 mm in length, making it the largest fluke that infects humans. The lifecycle involves eggs passed in feces that hatch in water and infect snail intermediate hosts, developing through sporocyst and redia stages before releasing cercariae that encyst on aquatic plants. Humans and pigs become infected by eating these metacercariae.
Leishmania is a genus of protozoan parasites that causes leishmaniasis, a vector-borne disease spread by sandfly bites. It exists in two forms: amastigotes within host cells and promastigotes in sandflies. The disease manifests as visceral, cutaneous, or mucocutaneous leishmaniasis depending on the infected tissues. Visceral leishmaniasis affects internal organs while cutaneous primarily affects the skin. Diagnosis involves detecting the parasites microscopically or through culture. Treatment involves pentavalent antimonials, amphotericin B, or miltefosine. Control relies on reducing sandfly and reservoir populations through insecticides and treating
Fasciola hepatica, commonly known as the sheep liver fluke, is a parasitic flatworm that infects the livers of sheep and cattle. It can also infect humans. The adult fluke lives in the bile ducts of the liver and lays eggs that pass in the feces. The life cycle requires an intermediate snail host to continue development. People become infected by ingesting metacercariae encysted on aquatic plants like watercress. Clinical symptoms in humans range from fever and abdominal pain during migration to liver damage and obstruction of the bile ducts in chronic infections. Diagnosis is confirmed by finding characteristic eggs in stool or biopsy samples. Treatment involves medications like triclabendazole.
The document discusses the diagnosis of trypanosomes from a blood sample. It provides background on trypanosomes and the diseases they cause. It then details the procedures used to diagnose the sample, including wet smear, thin smear, thick smear and buffy coat tests. Observations from each test are provided. The document concludes with a discussion of trypanosome morphology, life cycle, transmission, and the diseases they cause in humans and animals.
Necator americanus is a parasitic nematode known as the New World hookworm that infects humans. It lives in the small intestine and is a leading cause of iron-deficiency anemia in children in developing countries. The infective larvae penetrate the skin, travel to the lungs and intestines, and mature into adults that attach to the intestinal wall and suck blood, causing symptoms like abdominal pain and diarrhea. Eggs are passed in feces and can develop into infective larvae that can survive for weeks in soil, continuing the life cycle.
Fasciola hepatica, also known as the common liver fluke, is a parasitic flatworm that infects the livers of sheep and cattle. Its complex life cycle involves freshwater snails acting as intermediate hosts. Humans can become accidentally infected by consuming raw freshwater plants containing the fluke larvae. The flukes mature and reproduce in the bile ducts of the liver, causing a disease called fascioliasis. Symptoms range from asymptomatic to abdominal pain and liver damage. Diagnosis involves examining stool samples for fluke eggs or conducting imaging tests and antibody tests. Treatment primarily involves administering deworming medications like triclabendazole or bithionol.
Leishmania is a protozoan parasite that causes leishmaniasis, which exists in three main clinical forms: visceral leishmaniasis, cutaneous leishmaniasis, and mucocutaneous leishmaniasis. It is transmitted by the bite of infected female phlebotomine sand flies. Visceral leishmaniasis, the most severe form, affects the internal organs and is fatal if left untreated. Cutaneous leishmaniasis causes skin sores, while mucocutaneous leishmaniasis additionally affects the mucous membranes of the mouth and throat. Leishmania has a complex life cycle alternating between the sand fly vector and mammalian hosts.
Fasciolopsis buski is a large intestinal fluke that infects humans and pigs. It lives in the small intestine of its hosts and can measure up to 80 mm in length, making it the largest fluke that infects humans. The lifecycle involves eggs passed in feces that hatch in water and infect snail intermediate hosts, developing through sporocyst and redia stages before releasing cercariae that encyst on aquatic plants. Humans and pigs become infected by eating these metacercariae.
Leishmania is a genus of protozoan parasites that causes leishmaniasis, a vector-borne disease spread by sandfly bites. It exists in two forms: amastigotes within host cells and promastigotes in sandflies. The disease manifests as visceral, cutaneous, or mucocutaneous leishmaniasis depending on the infected tissues. Visceral leishmaniasis affects internal organs while cutaneous primarily affects the skin. Diagnosis involves detecting the parasites microscopically or through culture. Treatment involves pentavalent antimonials, amphotericin B, or miltefosine. Control relies on reducing sandfly and reservoir populations through insecticides and treating
Fasciola hepatica, commonly known as the sheep liver fluke, is a parasitic flatworm that infects the livers of sheep and cattle. It can also infect humans. The adult fluke lives in the bile ducts of the liver and lays eggs that pass in the feces. The life cycle requires an intermediate snail host to continue development. People become infected by ingesting metacercariae encysted on aquatic plants like watercress. Clinical symptoms in humans range from fever and abdominal pain during migration to liver damage and obstruction of the bile ducts in chronic infections. Diagnosis is confirmed by finding characteristic eggs in stool or biopsy samples. Treatment involves medications like triclabendazole.
The document discusses the diagnosis of trypanosomes from a blood sample. It provides background on trypanosomes and the diseases they cause. It then details the procedures used to diagnose the sample, including wet smear, thin smear, thick smear and buffy coat tests. Observations from each test are provided. The document concludes with a discussion of trypanosome morphology, life cycle, transmission, and the diseases they cause in humans and animals.
Necator americanus is a parasitic nematode known as the New World hookworm that infects humans. It lives in the small intestine and is a leading cause of iron-deficiency anemia in children in developing countries. The infective larvae penetrate the skin, travel to the lungs and intestines, and mature into adults that attach to the intestinal wall and suck blood, causing symptoms like abdominal pain and diarrhea. Eggs are passed in feces and can develop into infective larvae that can survive for weeks in soil, continuing the life cycle.
Fasciola hepatica, also known as the common liver fluke, is a parasitic flatworm that infects the livers of sheep and cattle. Its complex life cycle involves freshwater snails acting as intermediate hosts. Humans can become accidentally infected by consuming raw freshwater plants containing the fluke larvae. The flukes mature and reproduce in the bile ducts of the liver, causing a disease called fascioliasis. Symptoms range from asymptomatic to abdominal pain and liver damage. Diagnosis involves examining stool samples for fluke eggs or conducting imaging tests and antibody tests. Treatment primarily involves administering deworming medications like triclabendazole or bithionol.
This document summarizes information about malaria in Bangladesh, including:
- The main Plasmodium species that cause malaria in different regions of Bangladesh. P. vivax causes benign tertian malaria in flat lands, while P. falciparum causes malignant tertian malaria in hilly areas.
- The life cycle of Plasmodium, which involves both an intermediate human host and definitive mosquito host.
- The pathogenesis of malaria, including the infective forms, hosts, vectors, reservoirs, and modes of transmission.
- The clinical features of malaria, including periodic fevers, anemia, splenomegaly, and potential complications like cerebral malaria and blackwater fever.
- Methods for laboratory
This document describes Leishmania donovani, the parasite that causes visceral leishmaniasis or kala-azar. It discusses the parasite's classification, life cycle within human and sand fly hosts, geographic distribution, morphology in amastigote and promastigote forms, transmission via phlebotomine sand fly bites, pathology in organs like the spleen and liver, clinical features of kala-azar infection, and treatments like liposomal amphotericin B and miltefosine. It also briefly summarizes Leishmania species complexes that cause cutaneous and mucocutaneous leishmaniasis.
Wuchereria bancrofti is a parasitic roundworm that causes lymphatic filariasis. It is transmitted between humans via mosquitoes and infects the lymphatic system. Left untreated, the infection can lead to elephantiasis. The adult worms live in human lymphatic vessels and release microfilariae that show diurnal periodicity in the blood. The complex life cycle involves the human host and mosquito vectors. Prevention focuses on avoiding mosquito bites through insect repellents and nets.
This document provides information about malaria, including what it is, the species that cause it in humans, its prevalence and severity, symptoms, life cycle, transmission, immunity, diagnosis and treatment. Malaria is a mosquito-borne infectious disease caused by Plasmodium parasites. Four species infect humans: P. vivax, P. malariae, P. falciparum, and P. ovale. It is very common in developing countries, with 300-500 million cases and over 1 million deaths per year. Symptoms include periodic fevers, chills, sweating and flu-like illness. The parasite has stages in both humans and mosquitoes during its life cycle. Diagnosis is by blood smear microscopy
This document provides information about Plasmodium vivax, the parasite that causes benign tertian malaria. P. vivax originated in Asia and is mostly found in Asia, Latin America, and parts of Africa. It has dormant liver stages called hypnozoites that can activate months or years later to cause relapse. Symptoms include fever, chills, sweats, abdominal pain, nausea and vomiting. Diagnosis involves blood film examination, rapid tests, culture and PCR. Treatment includes chloroquine and primaquine which target the blood and liver stages respectively. Prevention involves chemoprophylaxis with drugs like chloroquine and mosquito control measures.
Nematodes are elongated, cylindrical worms that are bilaterally symmetrical. They vary in size from millimeters to over a meter in length. Most nematodes live freely in freshwater, seawater, or soil, though some are parasitic. Nematodes are classified based on their habitat and mode of infection, and whether they lay eggs or produce live larvae. They pass through six developmental stages from egg to adult. Parasitic nematodes cause tissue damage through mechanical disruption and toxicity, with the level of damage depending on factors like species, worm burden, habitat, and host immune response.
The document summarizes information about Plasmodium vivax, one of the protozoan parasites that cause malaria in humans. It is transmitted by Anopheles mosquitoes and has a complex life cycle involving liver and blood stages. Around 40% of the world's population lives in areas where P. vivax is endemic, and it causes an estimated 3-500 million clinical cases and 1.5-2.7 million deaths annually, mostly in Africa.
Giardia lamblia is a protozoan parasite that infects the small intestine. It exists in two forms - a trophozoite, which is the vegetative form, and a cyst, which is the infective form. The cyst is passed in feces and can remain viable in soil and water for weeks. Infection occurs when cysts are ingested through contaminated food or water. In the small intestine, cysts hatch into trophozoites which colonize the duodenum and jejunum. Symptoms include diarrhea, abdominal pain, and malabsorption. Diagnosis is made by identifying cysts or trophozoites in stool samples or small intestine samples. Treatment involves antibiotics like metronidaz
Tick-borne parasitic infections are caused by protozoan parasites transmitted through tick bites. The most common infections are babesiosis caused by Babesia parasites and theileriosis caused by Theileria species. These infections affect both animals and humans, causing symptoms ranging from fever and fatigue to enlarged lymph nodes. Diagnosis involves identifying the parasites in blood smears or tissue samples. Treatment consists of anti-parasitic drugs. Prevention strategies focus on controlling tick populations and avoiding tick bites through environmental and personal protective measures.
This document discusses Leishmania, the protozoan parasite that causes Leishmaniasis. It is transmitted by sandflies. Leishmaniasis is the second largest parasitic killer in the world after malaria. The document describes the classification, morphology, life cycle, clinical manifestations, diagnosis and treatment of Leishmania parasites and the diseases they cause. Key points covered include the different Leishmania species, the diseases they cause (visceral, cutaneous, mucocutaneous leishmaniasis), their diagnosis using blood smears, splenic aspirates or lymph node samples, and their treatment with drugs such as sodium stibogluconate or amphotericin B.
Plasmodium is a unicellular parasite that causes malaria in humans. It is transmitted via the bite of infected female Anopheles mosquitoes. Plasmodium has a complex life cycle involving sexual reproduction in mosquitoes and asexual reproduction in humans. When an infected mosquito bites a human, sporozoites enter the liver and later infect red blood cells, causing symptoms such as fever, chills, and anemia. Diagnosis involves examining blood smears under a microscope to identify the Plasmodium species and stages. Treatment involves antimalarial drugs, while control relies on preventing mosquito bites and reducing mosquito populations.
Reoviridae is a family of viruses that includes orthoreoviruses, rotaviruses, orbiviruses, and coltiviruses. They are non-enveloped viruses with double-layered protein capsids and segmented double-stranded RNA genomes. Rotaviruses are the most common cause of severe diarrhea in infants and young children worldwide. They replicate in the cytoplasm of intestinal cells. Orbiviruses commonly infect insects and can be transmitted to vertebrates by insects, causing diseases like bluetongue in sheep. While reoviruses are ubiquitous, their role in human disease is unclear.
This document provides an overview of Leishmaniasis, caused by protozoan parasites of the genus Leishmania. It discusses the life cycle of Leishmania donovani, the causative agent of visceral leishmaniasis (VL) or kala-azar. L. donovani has two forms - the amastigote form found within macrophages in humans and other mammals, and the promastigote form found in the sandfly vector. The sandfly transmits L. donovani between humans during feeding. In humans, L. donovani infects the reticuloendothelial system causing enlargement of the spleen, liver and bone marrow. Clinical features of VL include
The document summarizes different types of mites including itch mites, house dust mites, and scrub typhus mites. Itch mites cause scabies in humans through burrowing under the skin. House dust mites are found worldwide in dust and are a common cause of allergies. Scrub typhus is transmitted by the larvae of trombiculid mites and causes a disease called scrub typhus in parts of Asia and Australia.
Host-Parasite relationship is the extreme case of animal association, in which both partners influence each others life by affecting each others metabolism and behaviour using different adaptive mechanisms in order to ensure their survival.
The topic is highly useful for MBBS students.
Trichinella is a neamtode, The disease is called as Trichinellosis/Trichinosis. This topic will be explaining about Morphology of Trichinella, mode of transmission, life cycle ,clinical features, lab diagnosis, treatment and its prevention.
There are over 100 species of Plasmodium, some of which can infect humans and cause malaria. The four main species that infect humans are P. vivax, P. ovale, P. malariae, and P. falciparum. P. falciparum causes the most severe form of malaria and is responsible for over 1 million deaths per year globally. The life cycle of Plasmodium involves both sexual and asexual reproduction in humans and mosquitoes. Female Anopheles mosquitoes transmit the parasite between humans during blood-feeding. Laboratory diagnosis of malaria is usually done by examining thick and thin blood smears under a microscope to look for the parasite in red blood cells. Treatment depends on the species,
This document provides information on Leishmania donovani, the parasite that causes visceral leishmaniasis or kala-azar. It discusses the history and classification of L. donovani, its life cycle between human and sandfly hosts, clinical symptoms of kala-azar including fever and spleen/liver enlargement, and methods for laboratory diagnosis and treatment/prevention.
This document summarizes information about malaria in Bangladesh, including:
- The main Plasmodium species that cause malaria in different regions of Bangladesh. P. vivax causes benign tertian malaria in flat lands, while P. falciparum causes malignant tertian malaria in hilly areas.
- The life cycle of Plasmodium, which involves both an intermediate human host and definitive mosquito host.
- The pathogenesis of malaria, including the infective forms, hosts, vectors, reservoirs, and modes of transmission.
- The clinical features of malaria, including periodic fevers, anemia, splenomegaly, and potential complications like cerebral malaria and blackwater fever.
- Methods for laboratory
This document describes Leishmania donovani, the parasite that causes visceral leishmaniasis or kala-azar. It discusses the parasite's classification, life cycle within human and sand fly hosts, geographic distribution, morphology in amastigote and promastigote forms, transmission via phlebotomine sand fly bites, pathology in organs like the spleen and liver, clinical features of kala-azar infection, and treatments like liposomal amphotericin B and miltefosine. It also briefly summarizes Leishmania species complexes that cause cutaneous and mucocutaneous leishmaniasis.
Wuchereria bancrofti is a parasitic roundworm that causes lymphatic filariasis. It is transmitted between humans via mosquitoes and infects the lymphatic system. Left untreated, the infection can lead to elephantiasis. The adult worms live in human lymphatic vessels and release microfilariae that show diurnal periodicity in the blood. The complex life cycle involves the human host and mosquito vectors. Prevention focuses on avoiding mosquito bites through insect repellents and nets.
This document provides information about malaria, including what it is, the species that cause it in humans, its prevalence and severity, symptoms, life cycle, transmission, immunity, diagnosis and treatment. Malaria is a mosquito-borne infectious disease caused by Plasmodium parasites. Four species infect humans: P. vivax, P. malariae, P. falciparum, and P. ovale. It is very common in developing countries, with 300-500 million cases and over 1 million deaths per year. Symptoms include periodic fevers, chills, sweating and flu-like illness. The parasite has stages in both humans and mosquitoes during its life cycle. Diagnosis is by blood smear microscopy
This document provides information about Plasmodium vivax, the parasite that causes benign tertian malaria. P. vivax originated in Asia and is mostly found in Asia, Latin America, and parts of Africa. It has dormant liver stages called hypnozoites that can activate months or years later to cause relapse. Symptoms include fever, chills, sweats, abdominal pain, nausea and vomiting. Diagnosis involves blood film examination, rapid tests, culture and PCR. Treatment includes chloroquine and primaquine which target the blood and liver stages respectively. Prevention involves chemoprophylaxis with drugs like chloroquine and mosquito control measures.
Nematodes are elongated, cylindrical worms that are bilaterally symmetrical. They vary in size from millimeters to over a meter in length. Most nematodes live freely in freshwater, seawater, or soil, though some are parasitic. Nematodes are classified based on their habitat and mode of infection, and whether they lay eggs or produce live larvae. They pass through six developmental stages from egg to adult. Parasitic nematodes cause tissue damage through mechanical disruption and toxicity, with the level of damage depending on factors like species, worm burden, habitat, and host immune response.
The document summarizes information about Plasmodium vivax, one of the protozoan parasites that cause malaria in humans. It is transmitted by Anopheles mosquitoes and has a complex life cycle involving liver and blood stages. Around 40% of the world's population lives in areas where P. vivax is endemic, and it causes an estimated 3-500 million clinical cases and 1.5-2.7 million deaths annually, mostly in Africa.
Giardia lamblia is a protozoan parasite that infects the small intestine. It exists in two forms - a trophozoite, which is the vegetative form, and a cyst, which is the infective form. The cyst is passed in feces and can remain viable in soil and water for weeks. Infection occurs when cysts are ingested through contaminated food or water. In the small intestine, cysts hatch into trophozoites which colonize the duodenum and jejunum. Symptoms include diarrhea, abdominal pain, and malabsorption. Diagnosis is made by identifying cysts or trophozoites in stool samples or small intestine samples. Treatment involves antibiotics like metronidaz
Tick-borne parasitic infections are caused by protozoan parasites transmitted through tick bites. The most common infections are babesiosis caused by Babesia parasites and theileriosis caused by Theileria species. These infections affect both animals and humans, causing symptoms ranging from fever and fatigue to enlarged lymph nodes. Diagnosis involves identifying the parasites in blood smears or tissue samples. Treatment consists of anti-parasitic drugs. Prevention strategies focus on controlling tick populations and avoiding tick bites through environmental and personal protective measures.
This document discusses Leishmania, the protozoan parasite that causes Leishmaniasis. It is transmitted by sandflies. Leishmaniasis is the second largest parasitic killer in the world after malaria. The document describes the classification, morphology, life cycle, clinical manifestations, diagnosis and treatment of Leishmania parasites and the diseases they cause. Key points covered include the different Leishmania species, the diseases they cause (visceral, cutaneous, mucocutaneous leishmaniasis), their diagnosis using blood smears, splenic aspirates or lymph node samples, and their treatment with drugs such as sodium stibogluconate or amphotericin B.
Plasmodium is a unicellular parasite that causes malaria in humans. It is transmitted via the bite of infected female Anopheles mosquitoes. Plasmodium has a complex life cycle involving sexual reproduction in mosquitoes and asexual reproduction in humans. When an infected mosquito bites a human, sporozoites enter the liver and later infect red blood cells, causing symptoms such as fever, chills, and anemia. Diagnosis involves examining blood smears under a microscope to identify the Plasmodium species and stages. Treatment involves antimalarial drugs, while control relies on preventing mosquito bites and reducing mosquito populations.
Reoviridae is a family of viruses that includes orthoreoviruses, rotaviruses, orbiviruses, and coltiviruses. They are non-enveloped viruses with double-layered protein capsids and segmented double-stranded RNA genomes. Rotaviruses are the most common cause of severe diarrhea in infants and young children worldwide. They replicate in the cytoplasm of intestinal cells. Orbiviruses commonly infect insects and can be transmitted to vertebrates by insects, causing diseases like bluetongue in sheep. While reoviruses are ubiquitous, their role in human disease is unclear.
This document provides an overview of Leishmaniasis, caused by protozoan parasites of the genus Leishmania. It discusses the life cycle of Leishmania donovani, the causative agent of visceral leishmaniasis (VL) or kala-azar. L. donovani has two forms - the amastigote form found within macrophages in humans and other mammals, and the promastigote form found in the sandfly vector. The sandfly transmits L. donovani between humans during feeding. In humans, L. donovani infects the reticuloendothelial system causing enlargement of the spleen, liver and bone marrow. Clinical features of VL include
The document summarizes different types of mites including itch mites, house dust mites, and scrub typhus mites. Itch mites cause scabies in humans through burrowing under the skin. House dust mites are found worldwide in dust and are a common cause of allergies. Scrub typhus is transmitted by the larvae of trombiculid mites and causes a disease called scrub typhus in parts of Asia and Australia.
Host-Parasite relationship is the extreme case of animal association, in which both partners influence each others life by affecting each others metabolism and behaviour using different adaptive mechanisms in order to ensure their survival.
The topic is highly useful for MBBS students.
Trichinella is a neamtode, The disease is called as Trichinellosis/Trichinosis. This topic will be explaining about Morphology of Trichinella, mode of transmission, life cycle ,clinical features, lab diagnosis, treatment and its prevention.
There are over 100 species of Plasmodium, some of which can infect humans and cause malaria. The four main species that infect humans are P. vivax, P. ovale, P. malariae, and P. falciparum. P. falciparum causes the most severe form of malaria and is responsible for over 1 million deaths per year globally. The life cycle of Plasmodium involves both sexual and asexual reproduction in humans and mosquitoes. Female Anopheles mosquitoes transmit the parasite between humans during blood-feeding. Laboratory diagnosis of malaria is usually done by examining thick and thin blood smears under a microscope to look for the parasite in red blood cells. Treatment depends on the species,
This document provides information on Leishmania donovani, the parasite that causes visceral leishmaniasis or kala-azar. It discusses the history and classification of L. donovani, its life cycle between human and sandfly hosts, clinical symptoms of kala-azar including fever and spleen/liver enlargement, and methods for laboratory diagnosis and treatment/prevention.
MALARIA – PATHOGENESIS AND COMPLICATIONS 1.pptxDrSamiyahSyeed
1. Malaria is caused by Plasmodium parasites and transmitted via the bites of infected Anopheles mosquitoes.
2. The malaria parasite has a complex life cycle alternating between human and mosquito hosts. In humans, the parasites multiply in the liver and then infect and destroy red blood cells.
3. Symptoms of malaria in humans include fever, chills, and flu-like illness, and differ depending on the Plasmodium species. P. falciparum causes the most severe form of malaria and is responsible for the vast majority of malaria deaths.
able of ContentsIntroductionObjectives of Giemsa stainPrincipleReagents UsedProcedureStaining procedure 1: Thin Film stainingStaining Procedure 2: Thick Film StainingResultsInterpretation/ConclusionApplications Giemsa stainAdvantagesLimitationsReferencesFour Charged in Plot to Kidnap an Iranian Journalist in New YorkIntroductionGiemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstrating the presence of parasites in malaria.It belongs to a group of stains known as Romanowsky stains. These are neutral stains made up of a mixture of oxidized methylene blue, azure, and Eosin Y and they performed on an air-dried slide that is post-fixed with methanol. Romanowsky stains are applied in the differentiation of cells, pathological examinations of samples like blood and bone marrow films and demonstration of parasites e.g malaria. There are four types of Romanoswsky stains:Giemsa stainJenner StainWright stainMay-Grunwald StainLeishman stainObjectives of Giemsa stainTo accurately prepare the Giemsa stain stock solutionTo stain and identify blood cellsTo differentiate blood cells nuclei from the cytoplasmPrincipleGiemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.Like any type of Romanowsky stains, it composed of both the Acidic and Basic dyes, in relation to affinities of acidity and basicity for blood cells. Azure and methylene blue, a basic dye binds to the acid nucleus producing blue-purple color. Eosin is an acidic dye that is attracted to the cytoplasm and cytoplasmic granules which are alkaline-producing red coloration. The stain must be buffered with water to pH 6.8 or 7.2, to precipitate the dyes to bind simple materials.Classically, Giemsa stain is a differential stain which is made up of a combination of reagents (Azure, Methylene blue, and Eosin dye) used widely in cytogenetics and histopathology for the diagnosis of:Malaria, spirochetes and other blood parasitesChlamydia trachomatis inclusion bodiesBorrelia sppYersinia pestisHistoplasma sppPneumocystis jiroveci cystsReagents UsedMethanolGiemsa powderGlycerinWater (Buffer)ProcedurePreparation of the Giemsa Stain Stock solution (500ml)Into 250ml of methanol, add 3.8g of Giemsa powder and dissolve.Heat the solution up to ~60oCThen, add 250ml of glycerin to the solution, slowly.Filter the solution and leave it to stand for about 1-2 months before use.Preparation of Working solutionAdd 10ml of stock solution to 80ml of distilled water and 10ml of methanolStaining procedure 1: Thin Film stainingOn a clean dry microscopic glass slide, make a thin film of the specimen (blood) and leave to air dry.dip the smear (2-3 dips) into pure methanol for fixation of the
Unveiling the Intricacies of Leishmania donovani: Structure, Life Cycle, Path...Dr. Asif Anas
All leishmania pass their life cycle in two hosts. In man and some other mammals (definitive host) they occur exclusively in the amastigote form, having an ovoid body containing a nucleus and kinetoplast. In the sand-fly (intermediate host), they occur in promastigote form, with a spindle-shaped body and a single flagellum arising from the anterior end.
Definitive host:
In the body of definitive host, Leishmanias are mostly found within macrophages, monocytes, neutrophils or endothelial cells. Within these cells they multiply by binary fission, producing numerous daughter cells that distend the cells and rupture them.
The document discusses several protists from the class Zoomastigota, including Trypanosoma brucei, Leishmania species, and Trichomonas vaginalis. It describes their morphology, life cycles, hosts, transmission methods, locations in the host body, and the diseases they cause. The diseases covered are African trypanosomiasis, leishmaniasis, Chagas disease, and trichomoniasis. Diagnosis and prevention methods for these diseases are also summarized.
Malaria is a protozoan disease transmitted by mosquitoes. It is caused by Plasmodium parasites and affects over 3 billion people worldwide, causing approximately 1,200 deaths per day. The most severe form is caused by P. falciparum. Malaria symptoms include fever, chills, vomiting and headaches. Severe malaria can involve cerebral malaria, hypoglycemia, acidosis, pulmonary edema, renal failure and other complications affecting multiple organ systems. Malaria in pregnancy poses risks of low birth weight, stillbirth and maternal mortality.
Leishmaniasis is a parasitic disease caused by Leishmania protozoa and transmitted by the bite of infected sandflies. It exists in three main forms: visceral, cutaneous, and mucocutaneous. Visceral leishmaniasis affects internal organs and is fatal without treatment. Cutaneous leishmaniasis causes skin lesions while mucocutaneous leishmaniasis can destroy mucosal tissues of the nose, mouth and throat. It is endemic in many parts of Africa, Asia and South America, infecting over 12 million people worldwide.
By the end of this presentation we’ll be able to learn about- -Geographical distribution of leishmania parasites- Know the different stages of leishmania parasites and their morphology.-Describe the lifecycle of leishmania.-Causes and pathogenesis of leishmania -Preventive measures of leishmaniasis
This document summarizes information about four pathogens - Leishmania donovani, Leishmania tropica, Trypanosoma cruzi, and Trypanosoma gambiense/rhodesiense. It describes their life cycles, which involve transmission between hosts via sandfly or tsetse fly vectors. It also discusses the diseases they cause, including visceral leishmaniasis, cutaneous leishmaniasis, Chagas disease, and sleeping sickness. Signs and symptoms are provided for each disease's acute and chronic stages. Methods for laboratory diagnosis focus on identifying the pathogens in blood, tissue, or spinal fluid samples via microscopy or serology.
Visceral leishmaniasis (VL), also known as kala-azar, is the most severe form of leishmaniasis caused by the protozoan parasite Leishmania donovani and transmitted by the infected sandflies. It characterized by irregular bouts of fever, substantial weight loss, swelling of the spleen and liver, and anaemia.
Leishmaniasis is caused by protozoan parasites of the genus Leishmania and is transmitted by sand flies. Kala-azar, also known as visceral leishmaniasis, is caused by L. donovani and presents with fever, weight loss, enlarged liver and spleen. It is diagnosed by identifying the parasites in bone marrow or spleen aspirates under microscopy. Treatment involves pentavalent antimony compounds. Control relies on vector control measures and treating infected individuals to reduce the reservoir and prevent epidemics. Some patients may later develop a skin condition called post-kala azar dermal leishmaniasis.
This document discusses two genera of parasites, Leishmania and Trypanosoma, that cause diseases in humans. It describes the four major Leishmania pathogens, their life cycles involving sandflies, clinical manifestations including visceral leishmaniasis and cutaneous leishmaniasis, and methods of laboratory diagnosis. It also discusses the three major Trypanosoma pathogens, their life cycles involving reduviid bugs or tsetse flies, diseases caused including Chagas disease and sleeping sickness, clinical features, and diagnosis. The document provides detailed information on the pathogenesis, epidemiology, symptoms and laboratory identification of infections caused by these important parasites.
Leishmaniasis is caused by protozoan parasites of the genus Leishmania. It is transmitted by sand fly bites and affects the reticuloendothelial system. There are three main clinical forms: visceral leishmaniasis which involves vital organs, cutaneous leishmaniasis causing skin lesions, and mucosal leishmaniasis affecting mucous membranes. Visceral leishmaniasis, if left untreated, can be fatal and involves enlargement of the spleen, liver and lymph nodes with pancytopenia. Diagnosis involves clinical signs, serology, microscopy and culture. Treatment depends on the geographical region but involves pentavalent antimonials, amphotericin B
1. Leishmaniasis is caused by protozoan flagellates of the genus Leishmania and affects 350 million people globally.
2. It manifests clinically as cutaneous leishmaniasis, mucocutaneous leishmaniasis, or visceral leishmaniasis.
3. The parasite has two forms - amastigotes found intracellularly in humans and promastigotes found in sandfly vectors. The sandflies transmit the infective promastigote form during blood feeding.
Malaria is caused by Plasmodium parasites and transmitted via the bites of infected Anopheles mosquitoes. The parasite has a complex life cycle alternating between human and mosquito hosts. In humans, it causes flu-like symptoms including fever, chills, and headaches during the blood stage of infection. Diagnosis is usually by microscopic examination of blood smears, and treatment depends on the infecting parasite species. Complications can occur if not treated promptly, with cerebral malaria being the most severe manifestation. Prevention relies on mosquito control and the use of prophylactic drugs for travelers visiting malaria-endemic regions.
1. The document discusses malaria, providing classifications of the Plasmodium parasite, details of its life cycle within human and mosquito hosts, methods of diagnosis, and strategies for treatment and prevention.
2. Key points covered include the routes of transmission from mosquitoes to humans, stages within the parasite's complex life cycle including pre-erythrocytic and erythrocytic schizogony, and the use of rapid antigen detection tests and microscopy to diagnose malaria.
3. Treatment focuses on artemisinin combination therapy, while prevention emphasizes insecticide use and insecticide-treated bed nets to control the mosquito vector.
Trypanosoma gambiense is a flagellate protozoan parasite that causes sleeping sickness in humans. It reproduces asexually in the blood and cerebrospinal fluid of humans. Its life cycle involves transmission between humans and tsetse flies. When an infected fly bites a human, it can transmit the parasite either mechanically or after the parasite undergoes development stages in the fly. The parasite multiplies in the blood and can invade tissues, causing fever and swelling of lymph nodes before invading the brain and causing lethargy. Drugs like suramin and pentamidine are used to treat the disease. Control involves eradicating tsetse flies and preventing transmission between hosts.
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Mechanisms and Applications of Antiviral Neutralizing Antibodies - Creative B...Creative-Biolabs
Neutralizing antibodies, pivotal in immune defense, specifically bind and inhibit viral pathogens, thereby playing a crucial role in protecting against and mitigating infectious diseases. In this slide, we will introduce what antibodies and neutralizing antibodies are, the production and regulation of neutralizing antibodies, their mechanisms of action, classification and applications, as well as the challenges they face.
ESA/ACT Science Coffee: Diego Blas - Gravitational wave detection with orbita...Advanced-Concepts-Team
Presentation in the Science Coffee of the Advanced Concepts Team of the European Space Agency on the 07.06.2024.
Speaker: Diego Blas (IFAE/ICREA)
Title: Gravitational wave detection with orbital motion of Moon and artificial
Abstract:
In this talk I will describe some recent ideas to find gravitational waves from supermassive black holes or of primordial origin by studying their secular effect on the orbital motion of the Moon or satellites that are laser ranged.
Authoring a personal GPT for your research and practice: How we created the Q...Leonel Morgado
Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
Microbial interaction
Microorganisms interacts with each other and can be physically associated with another organisms in a variety of ways.
One organism can be located on the surface of another organism as an ectobiont or located within another organism as endobiont.
Microbial interaction may be positive such as mutualism, proto-cooperation, commensalism or may be negative such as parasitism, predation or competition
Types of microbial interaction
Positive interaction: mutualism, proto-cooperation, commensalism
Negative interaction: Ammensalism (antagonism), parasitism, predation, competition
I. Mutualism:
It is defined as the relationship in which each organism in interaction gets benefits from association. It is an obligatory relationship in which mutualist and host are metabolically dependent on each other.
Mutualistic relationship is very specific where one member of association cannot be replaced by another species.
Mutualism require close physical contact between interacting organisms.
Relationship of mutualism allows organisms to exist in habitat that could not occupied by either species alone.
Mutualistic relationship between organisms allows them to act as a single organism.
Examples of mutualism:
i. Lichens:
Lichens are excellent example of mutualism.
They are the association of specific fungi and certain genus of algae. In lichen, fungal partner is called mycobiont and algal partner is called
II. Syntrophism:
It is an association in which the growth of one organism either depends on or improved by the substrate provided by another organism.
In syntrophism both organism in association gets benefits.
Compound A
Utilized by population 1
Compound B
Utilized by population 2
Compound C
utilized by both Population 1+2
Products
In this theoretical example of syntrophism, population 1 is able to utilize and metabolize compound A, forming compound B but cannot metabolize beyond compound B without co-operation of population 2. Population 2is unable to utilize compound A but it can metabolize compound B forming compound C. Then both population 1 and 2 are able to carry out metabolic reaction which leads to formation of end product that neither population could produce alone.
Examples of syntrophism:
i. Methanogenic ecosystem in sludge digester
Methane produced by methanogenic bacteria depends upon interspecies hydrogen transfer by other fermentative bacteria.
Anaerobic fermentative bacteria generate CO2 and H2 utilizing carbohydrates which is then utilized by methanogenic bacteria (Methanobacter) to produce methane.
ii. Lactobacillus arobinosus and Enterococcus faecalis:
In the minimal media, Lactobacillus arobinosus and Enterococcus faecalis are able to grow together but not alone.
The synergistic relationship between E. faecalis and L. arobinosus occurs in which E. faecalis require folic acid
Mending Clothing to Support Sustainable Fashion_CIMaR 2024.pdfSelcen Ozturkcan
Ozturkcan, S., Berndt, A., & Angelakis, A. (2024). Mending clothing to support sustainable fashion. Presented at the 31st Annual Conference by the Consortium for International Marketing Research (CIMaR), 10-13 Jun 2024, University of Gävle, Sweden.
TOPIC OF DISCUSSION: CENTRIFUGATION SLIDESHARE.pptxshubhijain836
Centrifugation is a powerful technique used in laboratories to separate components of a heterogeneous mixture based on their density. This process utilizes centrifugal force to rapidly spin samples, causing denser particles to migrate outward more quickly than lighter ones. As a result, distinct layers form within the sample tube, allowing for easy isolation and purification of target substances.
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...Sérgio Sacani
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Anti-Universe And Emergent Gravity and the Dark UniverseSérgio Sacani
Recent theoretical progress indicates that spacetime and gravity emerge together from the entanglement structure of an underlying microscopic theory. These ideas are best understood in Anti-de Sitter space, where they rely on the area law for entanglement entropy. The extension to de Sitter space requires taking into account the entropy and temperature associated with the cosmological horizon. Using insights from string theory, black hole physics and quantum information theory we argue that the positive dark energy leads to a thermal volume law contribution to the entropy that overtakes the area law precisely at the cosmological horizon. Due to the competition between area and volume law entanglement the microscopic de Sitter states do not thermalise at sub-Hubble scales: they exhibit memory effects in the form of an entropy displacement caused by matter. The emergent laws of gravity contain an additional ‘dark’ gravitational force describing the ‘elastic’ response due to the entropy displacement. We derive an estimate of the strength of this extra force in terms of the baryonic mass, Newton’s constant and the Hubble acceleration scale a0 = cH0, and provide evidence for the fact that this additional ‘dark gravity force’ explains the observed phenomena in galaxies and clusters currently attributed to dark matter.
PPT on Alternate Wetting and Drying presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
2. DISCOVERY
• 1900,Calcutta - Sir William Boog
Leishman discovered L.donovani
in spleen smear of a solider who
died of fever at Dum-Dum, India.
• The disease was locally known as
dum-dum or kala-azar.
3. • Charles Donovan, Irish doctor at
Madras Medical college of India
also reported same ovoid bodies in
other kala-azar patients and
published his discovery a few
weeks after Leishman.
• After examining the parasite using
Leishman’s stain these amastigotes
were known as Leishman –Donovan
bodies.
4. • Leishmaniasis is a globally important but neglected disease.
• Largely affects individual of low socio-economic level, mainly in
developing countries and can affect people of all age group.
• Currently, Leishmaniasis occur in 4 continents (endemic in 88 countries, 72
developing countries)
90% of all VL: Bangladesh, Brazil, India, Nepal & Sudan
90% of all MCL: Bolivia, Brazil & Peru
90% of all CL: Afghanistan, Brazil, Iran, Peru, SA & Syria
• Prevalence: 12 million people
• Population at risk: 350 million
5. • Overall infection is caused by
more than 20 species of
Leishmania parasites, which are
spread by approx. 30 species of
phlebotomine sand flies.
• Sand fly vectors generally most
active after evening, in the night
hours.
• Climate and geographical factors
also play role.
Recently, Nepal reported 5 new cases in Kalikot. The district is located above 650m above
from sea level, thereby supporting the hypothesis that sand fly can survive high altitude
regions. With this development, it may become much difficult to contain this disease.
6. CLINICAL MANIFESTATION & PERSPECTIVE
• 3 main clinical manifestations of the disease leishmaniasis
• D/f species contribute to these 3 types
• 3 types of Leishmaniasis are:
• Visceral leishmaniasis (VL) most lethal & severe type
• Cutaneous leishmaniasis (CL)
• Mucocutaneous leishmaniasis (MCL)
• 2 other rare types
• Diffuse cutaneous leishmaniasis (DCL)
• Post kala-azar dermal leishmaniasis (PKDL)
7.
8. Leishmania Donovani
• Hemoflagellate protozoan
• Intracellular parasite
• Insect vector: Sand fly
• Belonging to Phlebotomus genus in old world
Leishmania.
• Lutzomyia genus for new world Leishmania species
• Causes fatal vector – borne disease called
leishmaniasis (kala-azar).
• Second largest parasitic killer in the world,
after malaria.
• Life cycle: digenetic (alternates b/w 2 hosts)
LD Bodies
9. Habits and Habitat of Leishmania donovani
• It is an intracellular parasite of man
and other mammalian hosts.
• In man, intracellular amastigote
forms are found in
reticuloendothelial cells of the
spleen, bone marrow, leucocytes or
cells of the liver, intestinal mucosa,
and mesenteric lymph nodes.
• The promastigote form is found in
the midgut of sandfly or in artificial
culture.
10. Morphology of Leishmania donovani
Leishmanial or Amastigote stage
• + in reticuloendothelial cells / blood cells , vertebrates.
• microscopic, rounded, or oval in shape measuring 2-4 micrometer in
length.
• no free flagellum, it is greatly reduced, fibril-like, and lies embedded
in the cytoplasm.
• The nucleus is central or eccentric.
• The cell membrane is delicate
• Kinetoplast is rod-shaped or dot-like and lies at the right angle to
the nucleus.
• The axoneme is a delicate filament extending from the kinetoplast
to the margin of the body. It represents the foot of the flagellum.
• stained well with Giemsa or Wright stain.
• In a Giemsa stained preparation, the cytoplasm surrounded by a
limiting membrane appears pale blue. The nucleus relatively is
larger and stained red. The kinetoplast stained deep red.
• Amastigote divides by binary fission at 37°C.
Leptomonad or promastigote stage
• It is found in the midgut of the invertebrates host or sandfly.
• elongated, slender, and spindle-shaped measuring 15-20µ in length
and 1-2µ in width.
• A flagellum is long measures 15-28µ and free and arises from a minute
basal body situated near the anterior end.
• The flagellum does not curve around the body of the parasite and
therefore there is no undulating membrane.
• The nucleus is centrally placed.
• The kinetoplast lies transversely near the anterior end.
• A vacuole is present near the root of the flagellum
• With Leishman stain, the cytoplasm appears blue, the nucleus pink or
violet, and the kinetoplast bright red.
• Promastigote multiplies by binary fission at 27°C.
11. LIFE CYCLE
Hosts
• Leishmania is a digenetic parasite that requires 2 hosts for completion
of its life cycle.
• The primary host is a vertebrate or man, in which the parasite feeds
and multiplies asexually.
• The secondary host or vector is invertebrates or blood-sucking
insects or sand-fly, belonging to the genus Phlebotomus.
• Some mammals like dogs, jackals, gerbils, and squirrels also serve as
reservoir hosts in which the parasite does not undergo any change
but simply waits for its introduction into the human host.
12. (I) Life cycle in Man
• The parasite has two stages in its life cycle:
• Amastigote form occurs in humans and mammals.
• Promastigote form occurs in sandfly.
• L. donovani is transmitted to humans or other vertebrates by the bite of blood-sucking
sandfly Phlebotomus argentipes.
• The parasites introduced by sandfly into the human body are in the promastigote form.
• Some of the promastigote entering the blood circulation directly become destroyed.
• while those entering the reticuloendothelial system(liver, spleen, bone marrow, and lymph nodes )
change into amastigote or leishmanial forms.
• The amastigotes multiply by simple binary fusion inside the Reticuloendothelial system to form a
large number of amastigotes.
• When the number of parasite reaches 50 to 200 or even more, the host cell rupture.
• The liberated parasites are taken up by new host cells and the multiplication cycle is repeated so
that the reticuloendothelial system becomes progressively infected.
• Some of the free amastigotes are phagocytized by the neutrophils and monocytes(macrophages)
in the bloodstream.
• These heavily parasitized cells wander through the general blood circulation leading to a general
infection.
13. (II) Life cycle in sandfly
• When the sandfly sucks the blood of an infected person, it obtains free
amastigotes as well as the parasitized neutrophils and monocytes along with the
blood-meal.
• The parasite begins a process of transformation and the amastigotes change to
procyclic promastigotes and then to metacyclic promastigotes in the midgut of
the sandfly.
• These promastigotes multiply by longitudinal binary fusion and produce large
numbers of promastigotes completely filling the lumen of the gut.
• In 6 to 9 days, the number of parasites becomes enormous and heavily spread
into the pharynx and buccal cavity. The salivary glands are not infected.
• Transmission into a new host occurs when such a heavily infected sandfly bites
the host.
14.
15. Survival in host
• Phagolysosome of macrophages
are the niche of parasitic
protozoan Leishmania and causes
human leishmaniasis.
• Leishmania turned the extremely
harsh environment inside the host
macrophage into a shelter to most
likely hide from the host immune
system
• Once it identifies its location inside
phagolysosome, Leishmania starts
to transform to the intracellular
form, the amastigote.
• To block parasite invasion,
macrophages activate release of
(ROS) and synthesis of cytotoxic
nitric oxide (NO) by NO synthase.
• The latter requires a massive
conversion of arginine to NO, which
exhausts intracellular arginine.
• Parasites developed sensing
mechanism that monitor arginine
in the phagolysosome, a
mechanism that is essential for
their survival and ability to develop
into amastigotes.
16. • During phagocytosis of uninfected macrophages, it takes about 30 min for
lysosome markers to appear in a phagosome, whereas it takes more than
2h for lysosome markers to appear in promastigote-infected phagosomes.
• Lipophosphoglycan (LPG), a major component of the promastigote
surface.
• It plays a key role in the infective pathway
• by interfering with the pro-inflammatory host cell responses via binding of Toll-like
receptor (TLR) 2 and 4 on macrophages and NK cells
• delays the appearance of vacuolar ATPases in phagosome membranes until they fuse
with primary lysosomes.
• delays phagosome acidification and maturation.
• Sensing acidic pH is one of the two cues that signal parasite to enter the
phagolysosome and subsequently initiate promastigote differentiation
into amastigotes.
• Protein Kinase A Is the Differentiation Gatekeeper
17. ROLE OF HSP23
• Change of temperature during the transmission from sandflies to mammals
is both a key trigger for the progression of their life cycle and for elevated
synthesis of heat shock proteins, which have been implicated in their
survival at higher temperatures.
• HSP23 protects L. donovani against cell stress, elevated temperature,
unfolded protein stress and redox stress.
• Loss of HSP23 causes increased sensitivity to chemical stressors and
renders L. donovani non-viable at 37°C.
• HSP23-null mutants are non-infectious to primary macrophages in vitro
• Thus, HSP23 expression is a prerequisite for L. donovani survival at
mammalian host temperatures and a crucial virulence factor.
18. SYMPTOMS
Phase 1
• enlargement of liver
and spleen along with
acute fever.
Phase 2
• acute enlargements of
liver and spleen along
with low fever
Phase 3
• cachexia (irreversible
weight loss & muscle
wasting) with no
observed fever
Kala azar = reticuloendotheliosis
Additional Symptoms: night sweating, anemia, headache, fatigue, swollen lymph
nodes.
Skin become dry, rough & darkly pigmented.
PKDL, is a rare reoccurrence of VL, in some instance even after 20 years of primary
infection.
Also co-infection of Leishmaniasis along with HIV due to immune- compromised state,
can be seen.
19. PATHOGENICITY
• SPLEEN – most affected, enlarged, cut section is red or chocolate in color due to
dilated and engorged vascular spaces.
• LIVER – enlarged, but functioning not affected, nutmeg appearance.
• BONE MARROW – parasitized macrophages
• LYMPH NODES – lymphadenopathy
• Fatal after 2-3 years if not treated
• Immediate cause of death is usually an invasion of a secondary
pathogen that the body is unable to combat. (pancytopenia)
20. PREVENTION
• Suppress the reservoir: dogs, rats, gerbils, other small mammal and
rodents
• Suppress the vector: Sand fly
(Critical to preventing disease in stationary troop population)
• Prevent Sand fly bites: personal protective measures
• Importantly at night
• Sleeves down
• Repellent
• Permethrin treated nets and clothes
21. TREATMENT
• Without anti- parasitic treatment, the case fatality rate of clinical VL
(kala-azar) is estimated to be >90%.
• Mortality is often due to hemorrhagic or infectious complications.
• Liposomal amphotericin B (L- AMP) only drug US FDA approved
• Miltefosine - orally administered phosphocholine
• Paromomycin - injectable aminoglycoside, has been shown to be effective in
Indian VL. Approved by India govt. in 2006.
• Combination therapies have the potential advantages of shortening
the duration and cost.
L-AMB + Miltefosine
22. HOW THESE DRUGS WORKS?
Liposomal amphotericin B (L- AMP)
• The activity mechanism involves
the high affinity binding of
amphotericin B to 24-substituted
sterol, ergosterol.
• Ergosterol is the major component
of leishmanial cell membrane,
which in turns leads to formation
of aqueous pores.
• Pores increased permeability of
monovalent cations, anions and
other small metabolites and
ultimately leads to cell death
Miltefosine
• This drug was developed as an
antineoplastic agent against MBC*
& treatment with this result in 95%
& 91% cure rate of VL & CL.
• The activity mechanism involves
the alteration of GPI* synthesis,
ether lipid metabolism & signal
transduction.
• It also induce nitric oxide (NO) ,
which leads to killing of parasite
within the macrophages.
Paromomycin
• It is an aminoglycoside,
relatively new broad spectrum
drug.
• It inhibits the leishmanial
protein synthesis by promoting
ribosomal subunit association &
low Mg2+ conc. induced
dissociation is known.
• Studies also shown that strain of
L. donovani developing
resistance against it.
MBC*- metastatic breast cancer
GPI* – glycosylphosphatidylinositol anchor