Biosafety Level 3 is for work with indigenous or exotic agents that may cause serious disease through inhalation. Specific training is required for personnel, and all procedures with infectious materials must be done in a biosafety cabinet or other containment device. A BSL-3 laboratory has special engineering controls and stringent safety practices, equipment, and facility requirements to contain the infectious agents and protect personnel. These include restricted access, personal protective equipment, specific facility design features, and procedures for containment, decontamination, and medical management following any exposures.
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
According to the Centre Of Disease Control and Prevention (CDC), Biosafety is the application of safety precautions that reduce a laboratorian’s risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community.
Safety cabinets are intended to protect a laboratory worker from aerosols and airborne particles.
They will not protect the person from spillages and the consequences of mishandling and poor technique.
Aerosol particles of less than 5 µm in diameter and small droplets of 5–100 µm in diameter are not visible to the naked eye.
The laboratory worker is generally not aware that such particles are being generated and may be inhaled or may cross contaminate work surface materials.
BSCs, when properly used, have been shown to be highly effective in reducing laboratory-acquired infections and cross-contaminations of cultures due to aerosol exposures. BSCs also protect the environment.
Most BSCs use high efficiency particulate air (HEPA) filters in the exhaust and supply systems.
The exception is a Class I BSC, which does not have HEPA filtered supply air.
Biosafety is the application of safety precautions that reduce a Laboratory based risk of exposure to a potentially infectious material and limit contamination of the working and surrounding environment.
The primary principle of biosafety is “Containment”.
Containment
The action of keeping harmful things under control and within limits
Or
A series of safe methods for managing infectious bacteria in the laboratory.
According to the Centre Of Disease Control and Prevention (CDC), Biosafety is the application of safety precautions that reduce a laboratorian’s risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community.
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
The application of knowledge, techniques and equipment to prevent a personal laboratory and environmental exposure to potentially infectious agents or biohazard is known as biosafety.
Biosafety defines the containment conditions under which infectious agents can be safely manipulated.
The objective of containment is to confine biohazard and to reduce the potential exposure of the laboratory worker, persons outside of the laboratory, and the environment to potentially infectious agents.
Phụ lục 3 tiêu chuẩn GMP EU về sản xuất thuốc thú y miễn dịch bao gồm:
1. Hệ thống chất lượng.
2. Nhân sự trong nhà máy thuốc thú y miễn dịch.
3. Nhà xưởng, trang thiết bị nhà máy thuốc thú y miễn dịch.
4. Hệ thống phụ trợ nhà máy thuốc thú y miễn dịch.
5. Công nghệ sản xuất.
6. Hệ thống giám sát, quản lý môi trường sản xuất và quá trình vận hành.
7. Kiểm soát chất lượng thành phẩm đầu ra
Laboratory organization refers to the systematic arrangement and management of laboratory equipment, supplies, and space to ensure efficient and safe workflow. It involves the proper storage of chemicals, maintenance of equipment, labeling of samples, and overall cleanliness of the laboratory space.
Biosafety management, on the other hand, focuses on implementing measures to control and prevent the exposure of laboratory workers, the environment, and the public from potential biological hazards. It includes the adoption of safe work practices, the use of personal protective equipment (PPE), and the implementation of containment measures for biohazardous materials.
Food hygiene is more than cleanliness ......
Protecting food from risk of contamination, including harmful bacteria, poison and other foreign bodies.
Preventing any bacteria present multiplying to an extent which would result in the illness of consumers or the early spoilage of the food.
Destroying any harmful bacteria in the food by thorough cooking
or processing.
Discarding unfit or contaminated food.
T-Cell Activation
• Concept of immune response
• T cell-mediated immune response
• B cell-mediated immune response
I. Concept of immune response
• A collective and coordinated response to the introduction of foreign substances in an individual mediated by the cells and molecules in the immune system.
II. T cell-mediated immune response
• Cell-mediated immunity is the arm of the adaptive immune response whose role is to combat infection of intracellular pathogens, such as intracellular bacteria (mycobacteria, listeria monocytogens), viruses, protozoa, etc.
Major Histocompatibility Complex
MHC:
• Major Histocompatibility Complex
– Cluster of genes found in all mammals
– Its products play role in discriminating self/non-self
– Participant in both humoral and cell-mediated immunity
• MHC Act As Antigen Presenting Structures
• In Human MHC Is Found On Chromosome 6
– Referred to as HLA complex
• In Mice MHC Is Found On Chromosome 17
– Referred to as H-2 complex
• Genes Of MHC Organized In 3 Classes
– Class I MHC genes
• Glycoproteins expressed on all nucleated cells
• Major function to present processed Ags to TC
– Class II MHC genes
• Glycoproteins expressed on macrophages, B-cells, DCs
• Major function to present processed Ags to TH
– Class III MHC genes
• Products that include secreted proteins that have immune functions. Ex. Complement system, inflammatory molecules
Antigen Processing and Presentation MID
Antigens and “foreignness”
• Antigens (or, more properly, immunogens) have a series of features which confer immunogenicity.
• One of these features is “foreignness.”
• So, we can infer that – most often – antigens – ultimately – originate externally.
• (There are exceptions, of course. Some cells become transformed by disease [e. g., cancer] or by aging. In such instances, the antigens have an internal origin.)
Extinction of a particular animal or plant species occurs when there are no more individuals of that species alive anywhere in the world - the species has died out. This is a natural part of evolution. But sometimes extinctions happen at a much faster rate than usual. Natural Causes of Extinction.
Difference between In-Situ and Ex-Situ conservation
Conservation of biodiversity and genetic resources helps protect, maintain and recover endangered animal and plant species. There are mainly two strategies for the conservation of wildlife: In-situ conservation and Ex-situ conservation. Although, both the strategies aim to maintain and recover endangered species, they are different from each other. Let us see how they differ from each other!
Evolution Of Bacteria
Bacteria have existed from very early in the history of life on Earth. Bacteria fossils discovered in rocks date from at least the Devonian Period (419.2 million to 358.9 million years ago), and there are convincing arguments that bacteria have been present since early Precambrian time, about 3.5 billion years ago. Bacteria were widespread on Earth at least since the latter part of the Paleoproterozoic, roughly 1.8 billion years ago, when oxygen appeared in the atmosphere as a result of the action of the cyanobacteria. Bacteria have thus had plenty of time to adapt to their environments and to have given rise to numerous descendant forms.
Impact of Environment on Loss of Genetic Diversity and Speciation
Genetic variation describes naturally occurring genetic differences among individuals of the same species. This variation permits flexibility and survival of a population in the face of changing environmental circumstances. Consequently, genetic variation is often considered an advantage, as it is a form of preparation for the unexpected. But how does genetic variation increase or decrease? And what effect do fluctuations in genetic variation have on populations over time?
GENE ENVIRONMENT INTERACTION
Subtle differences in one person’s genes can cause them to respond differently to the same environmental exposure as another person. As a result, some people may develop a disease after being exposed to something in the environment while others may not.
As scientists learn more about the connection between genes and the environment, they pursue new approaches for preventing and treating disease that consider individual genetic codes.
How to store food in hot
The Good News
To maximize benefit of preservation, keep your food as fresh as possible for as long as possible. You can do this, even in the heat, by creating a “cooler” made from two basic terra cotta pots, one larger than the other. Put the smaller pot in the larger one, fill the gap with sand, and saturate the sand with water. Then cover it with a cloth. To add additional insulation from the heat, bury the pot up to its rim. The evaporation of moisture from the wet sand will cool the air around the food and help keep it fresh.
What is IUPAC naming?
In order to give compounds a name, certain rules must be followed. When naming organic compounds, the IUPAC (International Union of Pure and Applied Chemistry) nomenclature (naming scheme) is used. This is to give consistency to the names. It also enables every compound to have a unique name, which is not possible with the common names used (for example in industry). We will first look at some of the steps that need to be followed when naming a compound, and then try to apply these rules to some specific examples.
IUPAC Nomenclature
IUPAC nomenclature uses the longest continuous chain of carbon atoms to determine the basic root name of the compound. The root name is then modified due to the presence of different functional groups which replace hydrogen or carbon atoms in the parent structure.
Hybridization describes the bonding atoms from an atom's point of view. For a tetrahedral coordinated carbon (e.g. methane CH4), the carbon should have 4 orbitals with the correct symmetry to bond to the 4 hydrogen atoms.
INTRODUCTION:
Hybrid Orbitals
Developed by Linus Pauling, the concept of hybrid orbitals was a theory created to explain the structures of molecules in space. The theory consists of combining atomic orbitals (ex: s,p,d,f) into new hybrid orbitals (ex: sp, sp2, sp3).
1. Why Firefly give light during night?
2. Why atomic mass and Atomic numbers are given to elements ?
3. Why elements have been characterized and classified into different groups?
4. What is the transition of elements and what they play their role in elements stability?
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
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We specializes in exporting high quality Research chemical, medical intermediate, Pharmaceutical chemicals and so on. Products are exported to USA, Canada, France, Korea, Japan,Russia, Southeast Asia and other countries.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Triangles of Neck and Clinical Correlation by Dr. RIG.pptx
Biosafety level 3
1. Biosafety Level 3
BiosafetyLevel 3 is applicable to clinical,diagnostic,teaching,research,or production facilitieswhere
work is performedwith indigenousorexoticagents that may cause seriousor potentiallylethal
disease through the inhalationroute of exposure.Laboratory personnel mustreceive specifictraining
in handlingpathogenicand potentiallylethal agents,and must be supervisedbyscientistscompetent
in handlinginfectiousagentsand associated procedures.
All proceduresinvolvingthe manipulationof infectiousmaterialsmustbe conductedwithinBSCsor
otherphysical containmentdevices.
A BSL-3 laboratoryhas special engineeringanddesignfeatures.
The followingstandard and special safety practices,equipment,and facilityrequirementsapplyto
BSL-3.
A. Standard Microbiological Practices
1. The laboratorysupervisormustenforce the institutional policiesthatcontrol accesstothe
laboratory.
2. Personsmustwashtheirhandsafterworkingwithpotentiallyhazardousmaterialsandbefore
leavingthe laboratory.
3. Eating,drinking,smoking,handlingcontactlenses,applyingcosmetics,andstoringfoodfor
humanconsumptionmustnotbe permittedinlaboratoryareas.Foodmustbe storedoutside
the laboratoryarea incabinetsor refrigeratorsdesignatedandusedforthispurpose.
4. Mouth pipettingisprohibited;mechanicalpipettingdevicesmustbe used.
5. Policiesforthe safe handlingof sharps,suchas needles,scalpels,pipettes,andbrokenglassware
mustbe developedand implemented.Wheneverpractical,laboratorysupervisorsshouldadopt
improvedengineeringandworkpractice controlsthat reduce riskof sharpsinjuries.
Precautions,includingthose listedbelow,must always be takenwith sharp items.These include:
a. Careful managementof needlesandothersharpsare of primaryimportance.Needles
mustnot be bent,sheared,broken,recapped,removedfromdisposablesyringes,or
otherwise manipulatedbyhandbefore disposal.
b. Useddisposable needlesandsyringesmustbe carefullyplacedinconvenientlylocated
puncture-resistantcontainersusedforsharpsdisposal.
c. Non-disposablesharpsmustbe placedina hardwalledcontainerfortransporttoa
processingareafordecontamination,preferablybyautoclaving.
d. Brokenglassware mustnotbe handleddirectly.Instead,itmustbe removedusinga
brushand dustpan,tongs,or forceps.Plasticware shouldbe substitutedforglassware
wheneverpossible.
6. Performall procedurestominimize the creationof splashesand/oraerosols.
2. 1. Decontaminate worksurfacesaftercompletionof workandafterany spill orsplashof
potentiallyinfectiousmaterialwithappropriatedisinfectant.
2. Decontaminate all cultures, stocks,andotherpotentiallyinfectiousmaterialsbefore
disposal usinganeffective method.A methodfordecontaminatingall laboratorywastes
shouldbe available inthe facility,preferablywithinthe laboratory(e.g.,autoclave,
chemical disinfection, incineration,orothervalidateddecontaminationmethod).
Dependingonwhere the decontaminationwill be performed,the followingmethods
shouldbe usedpriorto transport:
a.Materialsto be decontaminatedoutside of the immediate laboratorymustbe
placedina durable,leakproof containerandsecuredfortransport.
b.Materialsto be removedfromthe facilityfordecontaminationmustbe packed
inaccordance withapplicable local,state,andfederal regulations.
3. A signincorporatingthe universalbiohazardsymbol mustbe postedatthe entrance to
the laboratorywheninfectiousagentsare present.Postedinformationmustincludethe
laboratory’sbiosafetylevel,the supervisor’sname (orotherresponsiblepersonnel),
telephonenumber,andrequiredproceduresfor enteringandexitingthe laboratory.
Agentinformationshouldbe postedinaccordance withthe institutional policy.
4. An effective integratedpestmanagementprogramisrequired.(See AppendixG.)
5. The laboratorysupervisormustensure thatlaboratorypersonnel receiveappropriate
trainingregardingtheirduties,the necessaryprecautionstopreventexposures,and
exposure evaluationprocedures.Personnel mustreceive annual updatesoradditional
trainingwhenprocedural orpolicychangesoccur.Personal healthstatusmayimpactan
individual’ssusceptibilitytoinfection,abilitytoreceive immunizationsorprophylactic
interventions.Therefore,all laboratorypersonnel andparticularlywomenof
childbearingage shouldbe providedwithinformationregardingimmune competence
and conditionsthatmaypredispose themtoinfection.Individualshavingthese
conditionsshouldbe encouragedtoself-identifytothe institution’shealthcare provider
for appropriate counselingandguidance.
B. Special Practices
1. All personsenteringthe laboratorymustbe advisedof the potential hazardsandmeetspecific
entry/exitrequirements.
2. Laboratory personnel mustbe providedmedical surveillance andofferedappropriate
immunizationsforagentshandledorpotentiallypresentinthe laboratory.
3. Each institutionshouldconsiderthe needforcollectionandstorage of serumsamplesfromat-
riskpersonnel.
4. A laboratory-specificbiosafetymanual mustbe preparedandadoptedaspolicy.The biosafety
manual mustbe available andaccessible.
5. The laboratorysupervisormustensure thatlaboratorypersonnel demonstrateproficiencyin
standardand special microbiological practicesbefore workingwithBSL-3agents.
3. 6. Potentiallyinfectiousmaterialsmustbe placedinadurable,leakproof container during
collection,handling,processing,storage,ortransportwithinafacility.
7. Laboratory equipmentshouldbe routinelydecontaminated,aswell as,afterspills,splashes,or
otherpotential contamination.
a. Spillsinvolvinginfectiousmaterialsmustbe contained,decontaminated,andcleanedup
by staff properlytrainedandequippedtoworkwithinfectiousmaterial.
b. Equipmentmustbe decontaminatedbefore repair,maintenance,orremoval fromthe
laboratory.
8. 8. Incidentsthatmay resultinexposure toinfectiousmaterialsmustbe immediatelyevaluated
and treatedaccordingto proceduresdescribedinthe laboratorybiosafetymanual.All such
incidentsmustbe reportedtothe laboratorysupervisor.Medical evaluation,surveillance,and
treatmentshouldbe providedandappropriate recordsmaintained.
9. 9. Animalsandplantsnotassociatedwiththe workbeingperformedmustnotbe permittedin
the laboratory.
10. 10. All proceduresinvolvingthe manipulationof infectiousmaterialsmustbe conductedwithin
a BSC, or otherphysical containmentdevices.Noworkwithopenvesselsisconductedonthe
bench.Whena procedure cannotbe performedwithinaBSC,a combinationof personal
protective equipmentandothercontainmentdevices,suchasa centrifuge safetycuporsealed
rotor mustbe used.
C. Safety Equipment (Primary Barriers and Personal Protective Equipment)
1. All proceduresinvolvingthe manipulationof infectiousmaterialsmustbe conductedwithina
BSC (preferablyClassII or Class III),or other physical containmentdevices.
2. Workersinthe laboratorywhere protectivelaboratoryclothingwithasolid-front,suchastie-
back or wrap-aroundgowns,scrubsuits,orcoveralls.Protectiveclothingisnotwornoutside of
the laboratory.Reusable clothingisdecontaminatedbeforebeinglaundered.Clothingis
changedwhencontaminated.
3. Eye and face protection(goggles,mask,face shieldorothersplashguard) isusedforanticipated
splashesorspraysof infectiousorotherhazardousmaterials.Eye andface protectionmustbe
disposedof withothercontaminatedlaboratorywaste ordecontaminatedbefore reuse.
Personswhowearcontact lensesinlaboratoriesmustalsoweareye protection.
4. Glovesmustbe wornto protect handsfromexposure tohazardousmaterials.Glove selection
shouldbe basedonan appropriate riskassessment.Alternativestolatex glovesshouldbe
available.Glovesmustnotbe wornoutside the laboratory.Inaddition,BSL-3laboratory
workers:
a. Changesgloveswhencontaminated,glove integrityiscompromised,orwhenotherwise
necessary.Weartwopairsof gloveswhenappropriate.
b. Remove glovesandwashhandswhenworkwithhazardousmaterialshasbeen
completedandbefore leavingthe laboratory.
4. c. Do not washor reuse disposablegloves.Dispose of usedgloveswithother
contaminatedlaboratorywaste.Handwashingprotocolsmustbe rigorouslyfollowed.
5. Eye,face,and respiratoryprotectionmustbe usedinroomscontaininginfectedanimals.
D. Laboratory Facilities (Secondary Barriers)
1. Laboratory doors must be self-closing and have locks in accordance with the institutional
policies.The laboratorymustbe separatedfromareasthat are open to unrestricted traffic flow
withinthe building.Laboratoryaccessisrestricted.Accesstothe laboratory is through two self-
closingdoors.A clothingchange room(anteroom) maybe includedinthe passageway between
the two self-closing doors.
2. Laboratories must have a sink for hand washing. The sink must be hands-free or automatically
operated.Itshouldbe located near the exit door. If the laboratory is segregated into different
laboratories,asinkmustalsobe available for hand washing in each zone. Additional sinks may
be required as determined by the risk assessment.
3. The laboratorymust be designed so that it can be easily cleaned and decontaminated. Carpets
and rugs are not permitted.Seams,floors, walls, and ceiling surfaces should be sealed. Spaces
around doors and ventilation openings should be capable of being sealed to facilitate space
decontamination.
a. Floors must be slip resistant, impervious to liquids, and resistant to chemicals.
Considerationshouldbe giventothe installationof seamless,sealed,resilientor poured
floors, with integral cove bases.
b. Walls should be constructed to produce a sealed smooth finish that can be easily
cleaned and decontaminated.
c. Ceilings should be constructed, sealed, and finished in the same general manner as
walls.
d. Decontamination of the entire laboratory should be considered when there has been
gross contamination of the space, significant changes in laboratory usage, for major
renovations, or maintenance shut downs. Selection of the appropriate materials and
methodsusedtodecontaminate the laboratory must be based on the risk assessment.
4. Laboratory furniture mustbe capable of supportinganticipatedloadsanduses.Spacesbetween
benches, cabinets, and equipment must be accessible for cleaning.
a. Benchtops mustbe impervioustowaterandresistanttoheat,organicsolvents,acids,
alkalis,andotherchemicals.
b. Chairsusedin laboratoryworkmust be coveredwitha non-porousmaterialthatcan be
easilycleanedanddecontaminatedwithappropriate disinfectant.
5. All windows in the laboratory must be sealed.
6. BSCs mustbe installedsothat fluctuations of the room air supply and exhaust do not interfere
with proper operations. BSCs should be located away from doors, heavily traveled laboratory
areas, and other possible airflow disruptions.
5. 7. Vacuumlinesmustbe protectedwithHEPA filters,ortheirequivalent. Filters must be replaced
as needed. Liquid disinfectant traps may be required.
8. An eyewash station must be readily available in the laboratory.
9. A ducted air ventilation system is required. This system must provide sustained directional
airflowbydrawingairintothe laboratoryfrom“clean” areastoward“potentiallycontaminated”
areas.The laboratoryshall be designedsuchthatunderfailure conditionsthe airflow will not be
reversed.
a. Laboratory personnel must be able to verify directional airflow. A visual monitoring
device, which confirms directional airflow, must be provided at the laboratory entry.
Audible alarms should be considered to notify personnel of air flow disruption.
b. The laboratory exhaust air must not re-circulate to any other area of the building.
c. The laboratorybuildingexhaust air should be dispersed away from occupied areas and
from building air intake locations or the exhaust air must be HEPA filtered.
10. HEPA filter housings should have gas-tight isolation dampers, decontamination ports, and/or
bag-in/bag-out (with appropriate decontamination procedures) capability. The HEPA filter
housing should allow for leak testing of each filter and assembly. The filters and the housing
should be certified at least annually.
11. HEPA filtered exhaust air from a Class II BSC can be safely re-circulated into the laboratory
environment if the cabinet is tested and certified at least annually and operated according to
manufacturer’srecommendations.BSCscanalsobe connectedtothe laboratoryexhaustsystem
by either a thimble (canopy) connection or directly exhausted to the outside through a hard
connection. Provisions to assure proper safety cabinet performance and air system operation
mustbe verified.BSCsshouldbe certifiedatleastannuallyto assure correct performance. Class
III BSCs must be directly (hard) connected up through the second exhaust HEPA filter of the
cabinet.Supplyairmust be provided in such a manner that prevents positive pressurization of
the cabinet.
12. A method for decontaminating all laboratory wastes should be available in the facility,
preferably within the laboratory (e.g., autoclave, chemical disinfection, or other validated
decontamination method).
13. Equipmentthatmay produce infectious aerosols must be contained in primary barrier devices
that exhaustairthroughHEPA filtrationorotherequivalenttechnologybefore being discharged
into the laboratory. These HEPA filters should be tested and/or replaced at least annually.
14. Facility design consideration should be given to means of decontaminating large pieces of
equipment before removal from the laboratory.
15. Enhanced environmental and personal protection may be required by the agent summary
statement,risk assessment, or applicable local, state, or federal regulations. These laboratory
enhancementsmayinclude,for example, one or more of the following: an anteroom for clean
storage of equipmentandsupplieswithdress-in, shower-out capabilities; gas tight dampers to
facilitate laboratory isolation; final HEPA filtration of the laboratory exhaust air; laboratory
effluent decontamination; and advanced access control devices, such as biometrics.
6. 16. The BSL-3 facility design, operational parameters, and procedures must be verified and
documentedpriortooperation.Facilitiesmustbe re-verifiedanddocumentedatleast annually.
Reference
Book Name
Biosafety in Microbiologicaland Biomedical Laboratories
5th Edition
From Page No 38 to page 45