This document presents a simulation and validation of a cascade refrigeration system. It consists of two conventional vapor compression systems coupled through a cascade condenser. One uses CO2 as the refrigerant for low temperatures around -30C, while the other uses R404A at higher temperatures. The document describes simulating each system individually and comparing the heat loads calculated when each is run as an evaporator or condenser. It also describes an experimental analysis using a high temperature system with variable capacity compressors and primary/secondary condensers. The simulation tool predicted the COP with an average error of 4.4% compared to experimental results, validating the simulation approach.