Chromatography is a technique used to separate mixtures by using a mobile phase to carry the mixture through a stationary phase. High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses high pressure to pass a mobile phase through a column packed with adsorbent particles or porous beads. HPLC provides efficient separation of compounds in a mixture and is commonly used in chemistry laboratories and industries for analysis and purification of compounds.