This document provides an overview of column chromatography and gas chromatography instrumentation. Column chromatography separates components based on their differing rates of movement through a stationary phase packed in a column. Gas chromatography uses a mobile gas phase to carry vaporized sample components through a column coated with a liquid or solid stationary phase. Both techniques use instrumentation that includes columns, carrier gas systems, temperature control, sample injection ports, and detectors to separate and analyze sample components as they elute from the columns.
Introduction to chromatography, Definition of Chromatography, Types of column chromatography, Theory of chromatography, Practical considerations in column chromatography , Factors affecting efficiency of a column, Applications.
This presentation contains all the topics related to column chromatography. That includes introduction, principle,apparatus, experimental aspects of column chromatography, application of column chromatography, advantage and disadvantage of column chromatography with reference.
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
Introduction to chromatography, Definition of Chromatography, Types of column chromatography, Theory of chromatography, Practical considerations in column chromatography , Factors affecting efficiency of a column, Applications.
This presentation contains all the topics related to column chromatography. That includes introduction, principle,apparatus, experimental aspects of column chromatography, application of column chromatography, advantage and disadvantage of column chromatography with reference.
In this slide contains types of HPLC Columns, Plate theory and Van Deemter Equation.
Presented by : Malarvannan.M (Department of pharmaceutical analysis).
RIPER,anantpur.
in this slides contains principle and types of detectors used in Gas Chromatography.
Presented by: J.Vinay Krishna. (Department of industrial pharmacy),
RIPER, anantapur.
a substance can absorb any visible light or external radiation and then again emit it. this called fluorescence and the process of reduction in fluorescence intensity is called quenching. this presentation is all about quenching of fluorescence.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
Introduction and principle of glc, hplc
columns of hplc
columns of glc
detectors of glc
detectors of hplc
chromatography
classification of chromatography
gas liquid chromatography
high performance liquid chromatography
in this slides contains principle and types of detectors used in Gas Chromatography.
Presented by: J.Vinay Krishna. (Department of industrial pharmacy),
RIPER, anantapur.
a substance can absorb any visible light or external radiation and then again emit it. this called fluorescence and the process of reduction in fluorescence intensity is called quenching. this presentation is all about quenching of fluorescence.
In this slide contains Interference In Atomic Absorption Spectroscopy and applications.
Presented by: Shaik Gouse ul azam. ( department of pharmaceutical analysis.)
RIPER, anantpur.
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
PRINCIPLES of FT-NMR & 13C NMR
Fourier Transform
FOURIER TRANSFORM NMR SPECTROSCOPY
THEORY OF FT-NMR
13C NMR SPECTROSCOPY
Principle
Why C13-NMR is required though we have H1-NMR?
CHARACTERISTIC FEATURES OF 13 C NMR
Chemical Shifts
NUCLEAR OVERHAUSER ENHANCEMENT
Short-Comings of 13C-NMR Spectra
Introduction and principle of glc, hplc
columns of hplc
columns of glc
detectors of glc
detectors of hplc
chromatography
classification of chromatography
gas liquid chromatography
high performance liquid chromatography
Gas chromatography is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition.
chromatography general principles and comparison - specially about gas chrom...ShameerAbid
these sides discuss chromatography with special emphasis on gas chromatography
fundamentals
general principles
comparison of different chromatographic techniques
It is a multi-element analysis technique that will separate a sample into its constituent atoms and ions and excite it to a higher energy level.
Cause them to emit light with a distinct wavelength, which will be analyzed.
Similar to Instrumentation of column and gas chromatography (20)
Medical Technology Tackles New Health Care Demand - Research Report - March 2...pchutichetpong
M Capital Group (“MCG”) predicts that with, against, despite, and even without the global pandemic, the medical technology (MedTech) industry shows signs of continuous healthy growth, driven by smaller, faster, and cheaper devices, growing demand for home-based applications, technological innovation, strategic acquisitions, investments, and SPAC listings. MCG predicts that this should reflects itself in annual growth of over 6%, well beyond 2028.
According to Chris Mouchabhani, Managing Partner at M Capital Group, “Despite all economic scenarios that one may consider, beyond overall economic shocks, medical technology should remain one of the most promising and robust sectors over the short to medium term and well beyond 2028.”
There is a movement towards home-based care for the elderly, next generation scanning and MRI devices, wearable technology, artificial intelligence incorporation, and online connectivity. Experts also see a focus on predictive, preventive, personalized, participatory, and precision medicine, with rising levels of integration of home care and technological innovation.
The average cost of treatment has been rising across the board, creating additional financial burdens to governments, healthcare providers and insurance companies. According to MCG, cost-per-inpatient-stay in the United States alone rose on average annually by over 13% between 2014 to 2021, leading MedTech to focus research efforts on optimized medical equipment at lower price points, whilst emphasizing portability and ease of use. Namely, 46% of the 1,008 medical technology companies in the 2021 MedTech Innovator (“MTI”) database are focusing on prevention, wellness, detection, or diagnosis, signaling a clear push for preventive care to also tackle costs.
In addition, there has also been a lasting impact on consumer and medical demand for home care, supported by the pandemic. Lockdowns, closure of care facilities, and healthcare systems subjected to capacity pressure, accelerated demand away from traditional inpatient care. Now, outpatient care solutions are driving industry production, with nearly 70% of recent diagnostics start-up companies producing products in areas such as ambulatory clinics, at-home care, and self-administered diagnostics.
Leading the Way in Nephrology: Dr. David Greene's Work with Stem Cells for Ki...Dr. David Greene Arizona
As we watch Dr. Greene's continued efforts and research in Arizona, it's clear that stem cell therapy holds a promising key to unlocking new doors in the treatment of kidney disease. With each study and trial, we step closer to a world where kidney disease is no longer a life sentence but a treatable condition, thanks to pioneers like Dr. David Greene.
CHAPTER 1 SEMESTER V PREVENTIVE-PEDIATRICS.pdfSachin Sharma
This content provides an overview of preventive pediatrics. It defines preventive pediatrics as preventing disease and promoting children's physical, mental, and social well-being to achieve positive health. It discusses antenatal, postnatal, and social preventive pediatrics. It also covers various child health programs like immunization, breastfeeding, ICDS, and the roles of organizations like WHO, UNICEF, and nurses in preventive pediatrics.
The Importance of Community Nursing Care.pdfAD Healthcare
NDIS and Community 24/7 Nursing Care is a specific type of support that may be provided under the NDIS for individuals with complex medical needs who require ongoing nursing care in a community setting, such as their home or a supported accommodation facility.
How many patients does case series should have In comparison to case reports.pdfpubrica101
Pubrica’s team of researchers and writers create scientific and medical research articles, which may be important resources for authors and practitioners. Pubrica medical writers assist you in creating and revising the introduction by alerting the reader to gaps in the chosen study subject. Our professionals understand the order in which the hypothesis topic is followed by the broad subject, the issue, and the backdrop.
https://pubrica.com/academy/case-study-or-series/how-many-patients-does-case-series-should-have-in-comparison-to-case-reports/
CHAPTER 1 SEMESTER V - ROLE OF PEADIATRIC NURSE.pdfSachin Sharma
Pediatric nurses play a vital role in the health and well-being of children. Their responsibilities are wide-ranging, and their objectives can be categorized into several key areas:
1. Direct Patient Care:
Objective: Provide comprehensive and compassionate care to infants, children, and adolescents in various healthcare settings (hospitals, clinics, etc.).
This includes tasks like:
Monitoring vital signs and physical condition.
Administering medications and treatments.
Performing procedures as directed by doctors.
Assisting with daily living activities (bathing, feeding).
Providing emotional support and pain management.
2. Health Promotion and Education:
Objective: Promote healthy behaviors and educate children, families, and communities about preventive healthcare.
This includes tasks like:
Administering vaccinations.
Providing education on nutrition, hygiene, and development.
Offering breastfeeding and childbirth support.
Counseling families on safety and injury prevention.
3. Collaboration and Advocacy:
Objective: Collaborate effectively with doctors, social workers, therapists, and other healthcare professionals to ensure coordinated care for children.
Objective: Advocate for the rights and best interests of their patients, especially when children cannot speak for themselves.
This includes tasks like:
Communicating effectively with healthcare teams.
Identifying and addressing potential risks to child welfare.
Educating families about their child's condition and treatment options.
4. Professional Development and Research:
Objective: Stay up-to-date on the latest advancements in pediatric healthcare through continuing education and research.
Objective: Contribute to improving the quality of care for children by participating in research initiatives.
This includes tasks like:
Attending workshops and conferences on pediatric nursing.
Participating in clinical trials related to child health.
Implementing evidence-based practices into their daily routines.
By fulfilling these objectives, pediatric nurses play a crucial role in ensuring the optimal health and well-being of children throughout all stages of their development.
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
Growing Prevalence of Lifestyle Diseases
The rising incidence of lifestyle diseases such as diabetes, cardiovascular diseases, and cancer is a major trend driving the clinical trials market in India. These conditions necessitate the development and testing of new treatment methods, creating a robust demand for clinical trials. The increasing burden of these diseases highlights the need for innovative therapies and underscores the importance of India as a key player in global clinical research.
ICH Guidelines for Pharmacovigilance.pdfNEHA GUPTA
The "ICH Guidelines for Pharmacovigilance" PDF provides a comprehensive overview of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines related to pharmacovigilance. These guidelines aim to ensure that drugs are safe and effective for patients by monitoring and assessing adverse effects, ensuring proper reporting systems, and improving risk management practices. The document is essential for professionals in the pharmaceutical industry, regulatory authorities, and healthcare providers, offering detailed procedures and standards for pharmacovigilance activities to enhance drug safety and protect public health.
3. Column chromatography
• Column chromatography is basically a type of
adsorption chromatography techniques.
• Here the separation of components depends
upon the extent of adsorption to stationary
phase.
• Here the stationary phase is a solid material
packed in a vertical column made of glass or
metal.
4. Principle
• When a mixture of mobile phase and sample to
be separated are introduced from top of
the column, the individual components of
mixture move with different rates.
• Those with lower affinity and adsorption to
stationary phase move faster and eluted out first
while those with greater adsorption affinity
move or travel slower and get eluted out last.
5. Instrumentation
Column chromatography consists of
• A packed, three-dimensional stationary phase
inside a glass, plastic, or metal column and can
be used for both preparative and analytical
purposes.
6. Procedure:
• The stationary phase material is suitably moistened
with mobile phase and packed sufficiently in the
column with a cotton or asbestos pad at the bottom.
• The extract material or sample to be separated is
placed on the top of packed stationary phase with a
second cotton or asbestos pad in between.
• The mobile phase is poured into the column over the
sample. A collecting beaker is placed at the bottom
of column near the end to collect the elute.
7. Classification
Column chromatography is therefore classified
according to the type of fluid flow system used:
• Gravity chromatography
• Low-pressure chromatography
• Medium-pressure chromatography (including
fast protein liquid chromatography)
• High-pressure/high-performance liquid
chromatography (HPLC)
8. Gravity Chromatography
• Gravity chromatography uses gravity to pass
sample and buffers across the column resin.
• Small-volume columns (~1.5 ml) designed for
quick flowthrough by spinning in a
microcentrifuge provide a convenient method
for rapidly purifying many small samples.
10. Low-Pressure Chromatography
• Low-pressure chromatography systems are
operated at less than 50 psi (0.35 MPa).
• These systems require a sample pump and are
often equipped with fraction collectors, gradient
capabilities, and detectors to monitor column
elution.
12. Medium-Pressure Chromatography
• Medium-pressure chromatography is conducted
at operating pressures that are actually rather
high, up to 3,500 psi (24 MPa).
• Medium-pressure chromatography systems
often include additional capabilities such as
column switching valves, advanced gradient
capabilities, and multi-wavelength detectors.
13. Applications:
• Preparative and analytical chromatography of a
wide variety of molecules, ranging from
nonvolatile organics to nucleic acids, peptides,
and proteins.
14. High-Pressure Liquid
Chromatography (HPLC)
• Conducted at very high pressures — up to 5,000
psi (34 MPa).
• HPLC is a powerful analytical tool providing
high resolution and sensitivity, with the ability
to detect concentrations down to parts per
trillion while having very small sample
requirements.
15. Instrumentation
• Solvent Reservoir : Solvent must be deaerated prior
to use either by boiling or by applying a partial
vacuum to the solvent reservoir.
• Pump : The pump used in HPLC is pulseless (to
avoid pressure changes) with adjustable flow-rate.
They may pump solvents from more than one
reservoir.
• Pre-column : It contains same stationary phase as
that in separating column. It is used to remove any
impurities present in the mobile phase, which may
contaminate stationary phase in separating column.
16. • Injector : The sample to be separated is
introduced into injector by the help of a syringe.
It contains a valve which in one position allows
mobile phase to directly enter the separating
column. While, in the other position, it passes
mobile phase through a loop containing sample
mixture. Mobile phase flushes sample mixture
into separating column.
• Separating Column : It is made up of stainless
steel with length ranging from 10-100 cm and
diameter ranging from 2-6 mm. Micro bore
columns, often glass-lined, with diameter of
1 mm or less and length of 25 cm are also used.
The column can be placed in a thermo stated
oven.
17. • Detector : After exiting the column, the eluate
enters a flow-through detector, where it is
continuously monitored.
• Amplifier : The electrical signal obtained from the
detector is amplified and routed to recorder by
amplifier.
• Recorder : The recorder records the developed
chromatogram.
18. Applications:
• Preparative and analytical chromatography of a
wide variety of molecules, ranging from
nonvolatile organics to nucleic acids, peptides,
and proteins.
• Bio-Rad does not manufacture HPLC systems,
but we do carry a full selection of HPLC
columns.
19. Precautions:
1. Keep the column in a clean and dust free place.
2. Do not disturb the column till the separation is
complete.
3. Avoid gaps within the stationary phase packing.
20. Applications
• Column chromatography is best suited to
separate active principle from plant materials.
• In separation of compounds after organic
synthesis to obtain desired molecule.
• To separate or purify natural compound
mixtures like alkaloids, glycosides.
22. Gas Chromatography
• In gas chromatography, the components of a
sample are dissolved in a solvent and vaporized
in order to separate the analytes by distributing
the sample between two phases:
• a stationary phase and
• a mobile phase.
23. • The mobile phase is a chemically inert gas that
serves to carry the molecules of the analyte
through the heated column.
• The stationary phase is either a solid adsorbant,
termed gas-solid chromatography (GSC), or a
liquid on an inert support, termed gas-liquid
chromatography (GLC).
24. Instrumentation
Consist of
• Sample Injection
• Carrier Gas cylinder with pressure regulator
• Column Oven
• Open Tubular Columns and Packed Columns
• Detection Systems
25. Sample Injection
• A sample port is necessary
for introducing the sample
at the head of the column.
• A calibrated microsyringe is
used to deliver a sample
volume in the range of a few
microliters through a rubber
septum and into the
vaporization chamber.
26. Carrier Gas
• The carrier gas plays an important role, and
varies in the GC used.
• Carrier gas must be dry, free of oxygen and
chemically inert mobile-phase employed in gas
chromatography.
• Helium is most commonly used because it is
safer than, but comprable to hydrogen in
efficiency, has a larger range of flow rates and is
compatible with many detectors.
27. Gas cylinder with pressure regulator
• A pressure regulator is used to control the
amount of gas to be passed to column.
28. Column Oven
• The thermostated oven serves to control the temperature of
the column within a few tenths of a degree to conduct precise
work.
• The oven can be operated in two manners:
• isothermal programming
Or
temperature programming.
• In isothermal programming, the temperature of the column is
held constant throughout the entire separation.
• The optimum column temperature for isothermal operation
is about the middle point of the boiling range of the sample.
29.
30. Open Tubular Columns and Packed
Columns
• Open tubular columns, which are also known as
capillary columns, come in two basic forms.
• The first is a wall-coated open tubular (WCOT)
column and
• The second type is a support-coated open
tubular (SCOT) column.
31.
32. Detection Systems
• The detector is the device located at the end of
the column which provides a quantitative
measurement of the components of the mixture
as they elute in combination with the carrier
gas.
33. Types of Gas Chromatography
Detectors
Non-selective
• Responds to all compounds present in carrier
gas stream except the carrier gas itself
Selective
• Responds to range of compounds with a
common physical or chemical characteristic
Specific
• Responds to a single specific compound only
34. • Detectors can also be grouped into
concentration or mass flow detectors
• Concentration Dependent
• The response of such Gas
Chromatography detectors is proportional to the
concentration of the solute in the detector such
as TCD. Dilution of sample with makeup gas will
lower detector response.
• Mass Flow Dependent
• Signal is dependent on the rate at which solute
molecules enter the detector such as FID.
Response of such detectors is not affected by
makeup gas flow rate changes.
35. Desirable characteristics of
detectors
• Reproducible response to changes in eluent
composition in carrier gas stream
• High sensitivity
• Large linear dynamic range
• Low noise
• Small volume to avoid peak broadening and
resultant loss of resolution
• Preferably non – destructive
37. Flame Ionization Detector (FID)
• Mass sensitive detector
• Response depends on
conducting power of ions or
electrons produced on burning
of organic compounds in the
flame
• Selective detector but sample
detected must be combustible
• Large linear dynamic range
(107)
• No response to inorganic
and permanent gases such
as CO, CO2, NH3, CS2, N2,
etc.
• It is the most widely used
detector in Gas
Chromatography
38. Thermal Conductivity Detector (TCD)
• Non-destructive universal detector
• Response depends on the thermal conductivity
difference between the carrier gas and the eluted
components
• Wide dynamic range (107 – % to ppm levels)
• Responds also to inorganic gases such as CO,
CO2, NH3, CS2, N2, etc.
40. Applications
• Applications
• Gas chromatography is a physical separation method in where
volatile mixtures are separated.
• It can be used in many different fields such as
pharmaceuticals, cosmetics and even environmental toxins.
• Since the samples have to be volatile, human breathe, blood,
saliva and other secretions containing large amounts of
organic volatiles can be easily analyzed using GC.
• Knowing the amount of which compound is in a given sample
gives a huge advantage in studying the effects of human
health and of the environment as well.
• Air samples can be analyzed using GC.
• GC/MS is also another useful method which can determine
the components of a given mixture using the retention times
and the abundance of the samples.
41. REFERENCES
• www.chemwiki.ucdavis.edu.com
• www.lab-training.com
• www.bio-rad.com
• www.bheem.hubpages.com
• Modern Practice of Gas Chromatography
edited by Robert L. Grob, PhD, Eugene F. Barry,
PhD page no 37
• Liquid Column Chromatography: A Survey of
Modern Techniques and Applications
edited by K. Macek, Z. Deyl, J. Janák page no 57