A webinar for scientists interested in the use of implantable pumps as an alternative to repetitive injections when administering compounds to laboratory animals.
The administration of compounds and dosing methodologies are important considerations when planning experiments using laboratory animals. Injections are often used for animal dosing, but repeated injections over prolonged periods may be inadequate for some compounds (i.e. those with short half-lives) and can lead to great variations in serum concentrations. They are also stressful to the animal and inconvenient for the researcher. Implantable infusion pumps are useful drug delivery tools for preclinical research and drug development. They offer a reliable and convenient alternative to injections and other repetitive dosing methods. They allow greater control and accuracy in drug delivery, thus reducing dosing errors, ensuring stable dose levels and minimizing adverse effects. ALZET® Osmotic Pumps have been used in preclinical research for nearly four decades to successfully deliver a wide range of experimental agents. iPRECIO® programmable pumps have been developed more recently to facilitate highly demanding dosing requirements.
In this exclusive webinar sponsored by DURECT Corporation, Mr. José Gadea describes the features, benefits and applications of implantable pumps. Discussion focuses on clarifying the most common misconceptions circulating within the research community about implantable infusion pumps. This information is intended to help researchers make more informed decisions when choosing the most appropriate dosing method for their study.
Biopharmaceutic considerations in Drug Product DesignRiaz Islam
Drug product Design remain one of the most challenging aspects in formulation development. But nowadays formulation strategies have been far more successful in improving the bioavailability of the compounds with poor solubility, poor dissolution rate, and poor chemical stability in acidic environment. This article begins with a brief discussion on Physical and Chemical Properties of the Drug Impacting Oral Absorption. This article also reviews the Factors Contributing to Poor Aqueous Solubility. and a brief relationship between Physicochemical Properties and Drug Delivery System.
It is the presentation for Combinatorial Chemistry. this presentation should be helpful for B. Pharm students. It includes introduction, types, applications, advantages and disadvantages.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
the slid is about what is a nebulizer, types of the nebulizer, pneumatic nebulizer, ultrasonic nebulizer concentric tube nebulizer, cross flow, fritted disk, Babington etc.
THE PRODRUG DESIGNING FOR NEW SELECTION AND FORMULATION OF DRUG COMPATIBLE WITH API I.E. ACTIVE PHARMACUTICAL INGREDIENT, AND ITS EFFECT WHICH SHOULD BE 0. THE DRUG COMBINED WITH API AND AVILABLE IN MARKET AND DRUGS NEED TO BE COMBINE ARE ALSO DISCUSSED WITH ITS STRUCTURE AND SAR, AND COVERED AS PER THE SYLLABUS OF PCI.
Mountain Lab: Studying the effects of stress and extreme conditions on human ...InsideScientific
The human body is an amazingly complicated machine, capable of adapting and responding to various stressors and environmental conditions. Even in extreme situations the body is able to adjust core physiological processes and systems to ensure optimal function, and ultimately, survival. When studying human physiological response the most basic measurements, such as ECG and respiration, can hold huge amounts of information. But, their value is much greater when integrated with other physiological measurements such as blood pressure, oxygen saturation and respiratory gas concentrations.
However, accurate co-registration of physiological data is no trivial pursuit. Moreover, the complexity of such research endeavors is compounded when we venture out of traditional laboratory spaces and seek to study human response and adaptation in extreme environments. Sensors and systems must offer practical application and reliable data collection -- moreover, data storage and management is of critical importance.
In this webinar sponsored by ADInstruments, Dr. Trevor Day, Associate Professor of Physiology at Mount Royal University in Calgary Alberta, shares his research on the effects of tilt, exercise and high altitude on respiratory sinus arrhythmia (RSA). These case studies serve as representations of more complex applications of human physiologic monitoring, in particular, his trek to Everest Base Camp where he and his research team monitored and tracked acclimatization in the context of high altitude hypoxia. During this expedition multiple physiological measures were recorded simultaneously on both rest and exercise days in order to test for signs of altitude sickness. Dr. Day shares his experiences from this exciting study and others conducted at his lab at Mount Royal to offer perspective regarding the importance of being able to record and integrate multiple data streams simultaneously.
Gold Standard Physiological Measurements and Novel Drug Delivery Methods - Se...InsideScientific
A 2-part webinar for scientists interested in novel drug delivery methods for basic research, drug discovery and development. Learn about novel infusion technologies and how challenges in physiological monitoring and drug delivery are being overcome by implantable and programmable devices.
Session 1: The harmony of gold standard physiological monitoring with novel infusion technology: Better quality of science in preclinical models ranging from mice to marmosets.
Presenter: Christian Schnell, Novartis AG, Basel, Switzerland
Mr. Schnell presents data from multiple species that show how differences in blood pressure, heart rate and acute stress impact drug activity and how dosing a pre-trained animal can influence hemodynamic parameters.
Biopharmaceutic considerations in Drug Product DesignRiaz Islam
Drug product Design remain one of the most challenging aspects in formulation development. But nowadays formulation strategies have been far more successful in improving the bioavailability of the compounds with poor solubility, poor dissolution rate, and poor chemical stability in acidic environment. This article begins with a brief discussion on Physical and Chemical Properties of the Drug Impacting Oral Absorption. This article also reviews the Factors Contributing to Poor Aqueous Solubility. and a brief relationship between Physicochemical Properties and Drug Delivery System.
It is the presentation for Combinatorial Chemistry. this presentation should be helpful for B. Pharm students. It includes introduction, types, applications, advantages and disadvantages.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
the slid is about what is a nebulizer, types of the nebulizer, pneumatic nebulizer, ultrasonic nebulizer concentric tube nebulizer, cross flow, fritted disk, Babington etc.
THE PRODRUG DESIGNING FOR NEW SELECTION AND FORMULATION OF DRUG COMPATIBLE WITH API I.E. ACTIVE PHARMACUTICAL INGREDIENT, AND ITS EFFECT WHICH SHOULD BE 0. THE DRUG COMBINED WITH API AND AVILABLE IN MARKET AND DRUGS NEED TO BE COMBINE ARE ALSO DISCUSSED WITH ITS STRUCTURE AND SAR, AND COVERED AS PER THE SYLLABUS OF PCI.
Mountain Lab: Studying the effects of stress and extreme conditions on human ...InsideScientific
The human body is an amazingly complicated machine, capable of adapting and responding to various stressors and environmental conditions. Even in extreme situations the body is able to adjust core physiological processes and systems to ensure optimal function, and ultimately, survival. When studying human physiological response the most basic measurements, such as ECG and respiration, can hold huge amounts of information. But, their value is much greater when integrated with other physiological measurements such as blood pressure, oxygen saturation and respiratory gas concentrations.
However, accurate co-registration of physiological data is no trivial pursuit. Moreover, the complexity of such research endeavors is compounded when we venture out of traditional laboratory spaces and seek to study human response and adaptation in extreme environments. Sensors and systems must offer practical application and reliable data collection -- moreover, data storage and management is of critical importance.
In this webinar sponsored by ADInstruments, Dr. Trevor Day, Associate Professor of Physiology at Mount Royal University in Calgary Alberta, shares his research on the effects of tilt, exercise and high altitude on respiratory sinus arrhythmia (RSA). These case studies serve as representations of more complex applications of human physiologic monitoring, in particular, his trek to Everest Base Camp where he and his research team monitored and tracked acclimatization in the context of high altitude hypoxia. During this expedition multiple physiological measures were recorded simultaneously on both rest and exercise days in order to test for signs of altitude sickness. Dr. Day shares his experiences from this exciting study and others conducted at his lab at Mount Royal to offer perspective regarding the importance of being able to record and integrate multiple data streams simultaneously.
Gold Standard Physiological Measurements and Novel Drug Delivery Methods - Se...InsideScientific
A 2-part webinar for scientists interested in novel drug delivery methods for basic research, drug discovery and development. Learn about novel infusion technologies and how challenges in physiological monitoring and drug delivery are being overcome by implantable and programmable devices.
Session 1: The harmony of gold standard physiological monitoring with novel infusion technology: Better quality of science in preclinical models ranging from mice to marmosets.
Presenter: Christian Schnell, Novartis AG, Basel, Switzerland
Mr. Schnell presents data from multiple species that show how differences in blood pressure, heart rate and acute stress impact drug activity and how dosing a pre-trained animal can influence hemodynamic parameters.
Using Touchscreen Operant Systems to Study Cognitive Behaviors in RodentsInsideScientific
In this exclusive webinar sponsored by Lafayette Instrument, experts discuss novel rodent Touchscreen systems, referred to as Bussey-Saksida Systems, in terms of the animal environment, various behavioral Tasks, data analysis, methodology and prescribed best practices. In addition, information on integrating Touchscreen behavioral Tasks with video tracking, optogenetics and electrophysiology is shared.
Background Information:
Translational neuroscience has driven the need to create validated rodent and primate touchscreen ‘Tasks’ that are designed to mimic similar tests in clinical research. These Tasks cover many different aspects of cognitive behavior, one such example being PAL (Paired Associate Learning), a task shown to be very sensitive to detecting early onset of Alzheimer’s disease in humans. This well established human task displays six different images in six locations on a touchscreen -- the subject has to remember where each image was shown. If a mistake is made the images are shown to the subject again, introducing learning memory into the Task.
In the rodent version of the Task, subjects are first trained to touch the screens, then to initiate a task, and finally to learn which locations each of three images belong. The animal environment is optimized to focus subject attention to the Task displayed. In addition, to better understand the cognitive processes at play in the touchscreen Tasks these systems have been further developed to integrate behavioural tests with either Electrophysiology recording, optogenetic stimulation or video tracking.
The Future of Metabolic Phenotyping Using data bandwidth to maximize N, analy...InsideScientific
Methods matter. In metabolic measurement, confidence in reproducible results relies heavily on the design of the system used to acquire data. In the field of translational metabolic and behavioral phenotyping there is critical demand for more – throughput, standardization, synchronization of diverse data streams, temporal resolution, efficiency of workflow, and verification of results. We compare continuous and switched metabolic measurement methodologies and explore applications that benefit most from continuous measurement.
In this exclusive webinar sponsored by Sable Systems International, experts contrast methodologies and discuss how to improve best practices in metabolic phenotyping. We show how advances in high-bandwidth metabolic measurement, as implemented in Promethion metabolic phenotyping systems, leverage a 60- to 1200-fold increase in temporal resolution and achieve synchrony with intake and other behavioral data.
Key Topics:
* Time-saving methodologies for increasing throughput in multiplexed or continuous metabolic phenotyping
* Evaluation criteria for selecting a metabolic measurement system
* How the home-cage advantage of a pull-mode system reliably increases animal safety while dramatically reducing stress on both the animal and the researcher
* How to improve the resolution, accuracy and versatility of metabolic data using water vapor measurement
* The importance of raw data retention in metabolic phenotyping
* How deep data field format leads to greater traceability, improved reliability and far greater data extraction versatility to address research objectives
* How exact metabolic costs can be assigned to transient activities, with important implications for studies of energy balance, obesity, drug kinetics and metabolic diseases
Gold Standard Physiological Measurements and Novel Drug Delivery Methods - Se...InsideScientific
A 2-part webinar for scientists interested in novel drug delivery methods for basic research, drug discovery and development. Learn about novel infusion technologies and how challenges in physiological monitoring and drug delivery are being overcome by implantable and programmable devices.
Session 2: Synthetic, Structural, and Mechanistic Investigations of Vitamin B12 Conjugates of the Anorectic Peptide PYY3-36
Presenter: Dr. Robert Doyle, Syracuse University & SUNY, Upstate Medical University
Dr. Robert Doyle talks about how vitamin B12 conjugation of Peptide YY3-16 decreases food intake compared to native Peptide – YY3-36 in male rats. Learn how challenges to peptide-based therapies, such as rapid clearance, ready degradation by hydrolysis/proteolysis and poor intestinal uptake and/or a need for blood brain barrier transport can be overcome by using vitamin B12 in the subcutaneously administered drug delivery device iPrecio.
Educational webinars are one of the best ways to engage your target audience. Be an educator, build your brand, and collect market intelligence all from the convenience of your office! We are talking about the most cost and time effective method for lead generation, lead nurturing, and driving sales.
Combining Cardiovascular, Respiratory and Neurobehavioral Endpoints for Effic...InsideScientific
An essential webinar for preclinical scientists that wish to learn how to integrate hemodynamic, respiratory and neurological measurements to study multiple biological systems simultaneously while benefiting from more efficient data collection and workflow in the laboratory.
In this case study webinar sponsored by Data Sciences International, Dr. Brian Roche of Charles River Laboratories and Jason Payseur of GlaxoSmithKline discuss advantages and challenges pertaining to the combination of physiologic monitoring technologies to collect respiratory, cardiovascular and neurological endpoints from a single animal subject.
Specifically, Dr. Roche presents an evaluation of the AllayTM restraint technology utilized in DSI Respiratory solutions versus other commonly used methods. Complimented with implantable telemetry, Dr. Roche shows how he examined the effects of each method on various cardiopulmonary parameters and discusses the benefits and challenges associated with the use of the AllayTM restraint. Jason Payseur presents his assessment of a novel rodent model that examines cardiovascular, respiratory and neurobehavioral endpoints at the same time. He investigates the surgical feasibility of this model and tests its reliability in measuring multiple physiologic endpoints using tool compounds with known physiological effects, caffeine and chlorpromazine.
Applied Biomechanics – a multifaceted approach to answering human movement qu...InsideScientific
Experts review the basic principles of biomechanics and how the study of human movement has evolved over time. Presenters highlight examples in applied kinematics, applied kinetics and applied neuromuscular/motor control and demonstrate how methodologies vary depending on the field of study or area of expertise.
Combining Optical Brain Imaging and Physiological Signals to Study Cognitive ...InsideScientific
In this exclusive webinar sponsored by BIOPAC Systems and fNIR Devices, Dr. Hasan Ayaz, Dr. Kurtulus Izzetoglu and Frazer Findlay present new research capabilities enabled through the integration of optical brain imaging technology and physiological recording systems.
Key topics covered during this webinar include physiological and physical principles of optical brain imaging, theory of operation, hardware and software integration, essential fNIR signal processing (demonstrated using fnirSoft analysis software), common field applications of fNIR imaging, why and how researchers can measure physiological data such as EDA, HR and ECG and acquistion procedures for co-registration of fNIR data and physiological monitoring signals using AcqKnowledge data acquisition and analysis software.
Why comprehensive surgical monitoring has become so crucial in today’s scientific research space and how the MouseMonitor™ S by Indus Instruments is designed to address these specific needs. Industry experts Dr. Merry Lindsey, Director at the Jackson Center for Heart Research, and Dr. Corey Reynolds, Director of the Mouse Phenotyping Core at Baylor College of Medicine, share their experience with the MouseMonitor™ S and reviewed how it is used as both a vital signs monitor and platform for more precise physiological measurements.
Wireless Infusion Pumps: Securing Hospitals’ Most Ubiquitous Medical DevicePriyanka Aash
Imagine being dependent on a wireless infusion pump to receive the correct dosage of life-supporting medication. Now imagine the implications, were that pump to be maliciously hacked. In this session learn more about how to successfully secure these medical devices, based on work being conducted at the National Cybersecurity Center of Excellence (NCCoE) with premier health care organizations.
(Source: RSA USA 2016-San Francisco)
Effectiveness and safety of CPNB and continuous local wound infusion
Basal infusion with PCA option
Types of pumps – elastomeric vs. electronic
Outpatient and home infusion pumps
The Evolution of Wireless Monitoring in The Life Sciences and Review of Indus...InsideScientific
Biotelemetry is without a doubt the preferred approach for obtaining physiological measurements from animal research models in the fields of physiology, pathophysiology, pharmacology, drug discovery, and drug safety assessment. Naturally, the increase in application and availability of wireless measurement devices has fostered new research previously impossible, and motivated the works of many confirming the benefits of implantable telemetry over tethered and restrained animal models. This new era in implantable telemetry, where competition is more the rule than the exception, will drive down costs and expand the range of applications in life science research. During this opening webseries lecture, Brian Brockway will review the evolution of wireless technology and provide insight in to new possibilities based on recent innovations in the market place. Following, Dr. Robert Hamlin will provide an in-depth review of wireless monitoring practices in physiology, drug-discovery, and safety pharmacology and toxicology and discuss current industry standards for testing new therapeutic entities through wireless collection of blood pressure, blood flow, respiratory function, and ECG measurements.
Measuring Energy Balance in Mice from VO2/VCO2, Food Intake and Activity DataInsideScientific
Metabolic research continues to have an increased need for simultaneous multi-parameter measurements of Respiratory Exchange Ratio (RER), animal activity, and food intake (caloric consumption) to properly asses compounds, proteins, and genes on how they influence metabolism. But temporal alignment of this data into meaningful conclusions can have a steep learning curve for the non-initiated, and selecting appropriate options for such a complicated system can be over whelming. This webinar aims to discuss the essential elements and how they are to be applied.
In this webinar sponsored by Columbus Instruments, Chris Adams provides an overview of essential parameters for studying energy expenditure in laboratory rodents. He reviews gas exchange using open circuit indirect calorimetry to measure VO2, VCO2, RER and energy expenditure; food/water intake using direct mass measurement for accumulative and/or bout monitoring for controlling caloric intake and for identifying diet preference; and activity using an IR photo-cell array and/or running wheel rotation for scoring locomotion, stereotypic behavior (grooming/scratching), resting behavior and wheel running behavior.
In addtion, Dr. Marta Fiorotto presents quantitative data acquired in her lab such as energy intake and energy expenditure while highlighting qualitative measurements such as spontaneous cage activity, voluntary activity and dietary-induced thermogenesis. She discusses the value of "secondary data" as well with a focus on RER and behaviors such as eating patterns, sleeping and exploratory drive. Finally, Dr. Fiorotto highlights key considerations for proper experimental design and to ensure data validity including habituation, animal-to-animal individuality, diets, environmental factors, instrument setup, timing of measurement and normalization protocols.
Improving Rodent Cardiovascular Research Outcomes with Integrated Surgical Mo...InsideScientific
An essential webinar for both beginning and seasoned cardiovascular researchers looking to improve hemodynamic study outcome consistency by monitoring vital signs during surgery.
Dr. James Clark of King's College London and Graham Sattler of Indus Instruments discuss methodology, surgical monitoring best-practices, and demonstrate how to improve study and animal outcomes for Pressure-Volume Loop Studies using integrated monitoring of vital signs including core body temperature, ECG, respiration rate, and blood oxygenation.
Discussions focus on how an integrated surgical heating and vital signs monitoring can significantly improve cardiovascular surgical outcomes by enabling real-time insights into overall physiologic and cardiovascular conditions before, during and after surgical procedures. Presenters also review how the MouseMonitor S can be integrated with Pressure-Volume Loop systems and a wide variety of research instrumentation used to study hemodynamics.
Michael Kinder provides insight on the underlying technologies used in startle systems and explains what researchers should know about sensor capabilities and limitations and how that knowledge can help create effective protocols, address calibration and properly analyze data.
The startle reflex in rodents is typically studied using startle chambers with integrated transducers to measure elicited responses to aversive stimuli. The importance of startle systems in pre-clinical research labs is evident based on the pervasive use of this technology in both academia and industry for applications such as behavioral phenotyping, pre-pulse inhibition studies and safety pharmacology and toxicology screening. As is true with any scientific instrument, understanding the underlying technology and inherent limitations is important when designing experimental protocols, setting up and calibrating the equipment and analyzing data.
Michael Kinder, System Designer at Kinder Scientific, presents advancements in modern startle systems that scientists should know. Topics include:
- What are the technologies available that scientists need to understand?
- What are the physical characteristics of the sensory assemblies?
- Does subject weight matter in startle response studies?
- Should scientists be comparing raw startle amplitude between animals of different sizes?
- Does the unit of measure matter?
- What does an analog only trial provide (or not provide)?
Making Optical and Electrophysiological Measurements in the Brain of Head-Fix...InsideScientific
A growing number of researchers are moving from reduced preparations such as dissociated cultured neurons or brain slices, to experimentation in live animals - in vivo - using advanced methods such as two-photon microscopy or combined optogenetics and patch-clamp recordings. In order to immobilize the animal during these challenging applications general anesthesia is often administered; however, the use of anesthetics greatly distorts brain function.
Is there a better way?
In this exclusive webinar sponsored by Neurotar Ltd, leading experts in the technology will discuss methodology, best-practices and show attendees how to immobilize the rodent’s head without restraining its body using the Mobile HomeCage™. The result is a controlled research environment for studying brain function in awake, freely-moving subjects with no stress to the animal. Discussion around how this technique can be applied to the study of neuronal plasticity, neurodegeneration, addiction, brain trauma and other pathophysiological conditions in longitudinal experiments will be included. Furthermore, presenters will demonstrate how this methodology is best combined with microscopy and electrophysiology techniques – all in vivo.
INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip.
This technology enables INTip solid phase extraction for easy sample preparation. The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
Basic Approach to Dissolution Method Development – Challenges and Regulatory ...Dr. Harshal Pawar
This presentation explains the theoretical as well as practical aspects of dissolution. It provides a systematic and scientific path for development of dissolution method for a new pharmaceutical product.
Parenterals, most useful presentation for GPAT aspirant and UG PG students of Pharmacy field. Details regarding parenteral routes, formulation consideration and quality control tests
Sagar Veterinary drug delivery system ppt.Sagar Goda
This article would be provided different dosage forms are used especially for veterinary and also provided the importance of veterinarian.It can also provided the European guidelines for medicinal products that used for Human and Veterinary. For discovering various animal diseases, new technological devices as well as treatments are also available in this article.
topic name is parental drug delivery system which include their use , advantages, disadvantages, necessities,
parentral routes of administration, formulation
bacterial growth, their phases
pyrogen testing
Join the experts as they discuss the use of accelerated solvent extraction and QuEChERS techniques for the extraction of pesticide residues from a diverse range of food samples. Tips and tricks for improving the extraction efficiency will be covered, along with selection criteria for each technique by sample type, assisting analysts in modifying existing methods or developing new methods to tackle their analytical challenges
Similar to Implantable Infusion Pumps: Insights For Your Next Animal Dosing Study (20)
Next-Generation Safety Assessment Tools for Advancing In Vivo to In Vitro Tra...InsideScientific
Join Prof. Victoria Hutter and Dr. Louis Scott as they showcase the application of high-content imaging and advanced cell lines for drug safety assessment.
Safety concerns play a significant role in the unsuccessful progression of candidate compounds in the later stages of drug development. Establishing the connection between in vitro endpoints and human health outcomes is essential.
In this webinar, Prof. Victoria Hutter and Dr. Louis Scott present a novel tool for in vitro safety assessment in drug development. The morph_ONE™ assay provides a human-centric approach to potentially fill specific regulatory gaps concerning safety issues. This tool is capable of profiling both human and rat alveolar macrophages, offering valuable insights for hazard identification and toxicity assessments. By bridging the divide between cellular effects and overall risk, it has the potential to enhance our understanding of safety-related aspects in drug development.
Key Topics Include:
- Explore distinct in vitro screening techniques for evaluating the safety of emerging inhaled products, facilitating early and informed decisions in compound selection and development.
- How high-content image analysis (HCIA) cell painting assays can be used as a forward-looking high-throughput screening tool, distinguishing unique response patterns in alveolar macrophages.
- Understand the use of the ImmuPHAGE™ and ImmuLUNG™ models in conducting customized evaluations focused on inhalation safety.
A Ready-to-Analyze High-Plex Spatial Signature Development Workflow for Cance...InsideScientific
In this webinar, Aditya Pratapa and Lorcan Sherry present a new workflow for analyzing multiplex immunoflurescence images.
Spatial Signatures are a new class of highly predictive biomarkers that measure the interactions and cellular densities of tumor and immune cells that compose the tumor microenvironment. Based on multiplex immunofluorescence, spatial signatures provide a deeper understanding of complex interactions between tumors and the immune system, enabling improved patient stratification for immunotherapies. A significant hurdle to date has been in developing a data analysis workflow that is straightforward and user-friendly to transform the data rich images into meaningful quantitative spatial signatures.
In this webinar, Aditya and Lorcan review the key features of the new PhenoImager HT 2.0 data analysis workflow. This workflow introduces a simplified framework from scanning to analyzing spectrally unmixed multiplex immunofluorescence images generated on the PhenoImager HT platform. The ready-to-analyze data can be directly imported into image analysis software such as Visiopharm. This presentation covers key aspects of data analysis elements such as image QC, segmentation, phenotyping, and verification – all essential for creating outputs that support the development of a spatial signature.
Key Topics Include:
- Understand Akoya’s new HT 2.0 data analysis workflow
- The challenges in multiplex immunofluorescence analysis and the use of AI and cell
lineage segmentation considerations
- Explore OracleBio’s image analysis workflow incorporating Visiopharm
- Evaluation of analysis data to facilitate spatial profiling and interpretation
Molecule Transport across Cell Membranes: Electrochemical Quantification at t...InsideScientific
In this webinar, Dr. Sabine Kuss will discuss the importance of transmembrane molecule exchange and how to detect and quantify membrane transport of molecules in cells.
Complex biological processes, such as the transport of molecules across cell membranes, are difficult to understand using purely biological methodologies. Investigating cellular transport processes is challenging, because of the highly complex chemical composition of cells and the diffusion of molecules in and around cells at low concentrations. The development and advancement of electroanalytical methods over the last two decades has enabled the monitoring of living cells and their interaction with the environment, including external stimuli, such as pharma-molecules.
This presentation emphasizes electrochemical and electrophysiological methods of detection and quantification but also makes a comparison to other bioanalytical approaches. Join us to discover a substantial diversity in methods used to monitor the transport of cell metabolites, crucial for cell survival, and pharmaceutical compounds, involved in cell characteristics such as drug resistance.
Key Topics Include:
- Understanding transmembrane molecule transport through bioanalytical methods
- Electrochemical approaches to monitor molecule transport across cell membranes
- What bioanalytical and especially electrochemical approaches can reveal
- Challenges associated with instrument limitations
Exploring Predictive Biomarkers and ERK1/2 Phosphorylation: A New Horizon in ...InsideScientific
In this webinar, Dr. Victor Arrieta highlights the link between p-ERK activation and improved survival in rGBM patients using anti-PD-1 immunotherapy.
Recurrent glioblastoma (rGBM) has displayed a varied response to anti-PD-1 immunotherapy, necessitating the identification of predictive biomarkers. Through extensive analyses and 3 clinical studies, we have identified that activation of the MAPK/ERK signaling pathway, particularly ERK1/2 phosphorylation (p-ERK), is associated with longer overall survival (OS) in rGBM patients receiving PD-1 blockade. Initially, enrichment of BRAF/PTPN11 mutations was reported in 30% of responsive rGBM patients, prompting the investigation of p-ERK as a potential marker beyond these mutations.
Our research has unraveled an association between p-ERK abundance and better clinical outcomes following PD-1 blockade, with p-ERK mainly localized in tumor cells. Notably, high p-ERK GBMs contained unique microglia and macrophage phenotypes with elevated MHC class II expression, suggesting a novel interplay between MAPK activation and the tumor immune microenvironment.
While these insights establish a pivotal role for p-ERK in predicting PD-1 blockade response in rGBM, the implementation in clinical settings calls for further validation and accuracy. Nonetheless, these findings pave the way for more personalized and effective immunotherapy strategies, emphasizing the significance of the tumor microenvironment and its interaction with therapeutic interventions in GBM.
Key Topics Include:
- The activation of the MAPK signaling pathway, specifically ERK1/2 phosphorylation (p-ERK), is identified as a predictive biomarker for longer overall survival in recurrent glioblastoma (eGBM) patients undergoing PD-1 blockade
- High p-ERK tumors in rGBM present a distinct myeloid cell phenotype with elevated MHC class II expression, signifying a connection between MAPK pathway activation and the immune microenvironment
- The implementation of p-ERK as a predictive biomarker in clinical settings requires further validation and exploration of variables impacting its evaluation
Exploring Estrogen’s Role in Metabolism and the Use of 13C-Labeled Nutrients ...InsideScientific
Dr. Reilly Enos and Dr. Eran Levin discuss estrogen's metabolic impact and how isotopic labeling and 13C-labeled nutrients can be used for animal physiology and nutrition research.
Reilly Enos, PhD – Harnessing the power of estrogen to regulate metabolic processes
Dr. Reilly Enos’ research focuses on the role that sex steroids and their receptors play in regulating metabolic processes, particularly in the setting of obesity. In this webinar, Dr. Enos will discuss his research on tissue-specific fluctuations of sex steroids throughout the estrous cycle in mice, provide insights into the importance of the quantity of estrogen necessary to impact physiological processes, as well as an understanding of the central versus peripheral effects of estrogen action.
Eran Levin, PhD – Unlocking Insights: Utilizing 13C Labeled Nutrients for Cutting-Edge Physiology and Nutrition Research
Dr. Eran Levin will discuss the potential of using 13C-labeled nutrients in physiology and nutrition research in animal models. Specifically, he will share practical tips for designing and conducting experiments using isotopic labeling techniques and demonstrate how they can provide unprecedented insights into metabolic pathways, nutrient utilization, and behaviors in both vertebrate and invertebrate models including insects, reptiles, and mammals.
Key Topics Include:
- The role that estrogen plays in regulating metabolic and behavioral processes in males and females
- The tissue-specific fluctuations of sex steroids throughout the estrous cycle
- Insight into the importance of tissue-specificity in developing hormonal therapies
- The importance of estrogen quantity in regulating physiological processes
- Understand the diverse range of 13C labeled nutrients available
- Specific applications of labeled amino acids in studies of protein metabolism, cellular signaling, and typical nutrient utilization
- How to integrate 13C labeling techniques with respirometry for a comprehensive assessment of metabolic processes, energy expenditure, and substrate utilization in animal models
- How to calculate metabolic rates in free-flying animals using 13C bicarbonate
Longitudinal Plasma Samples: Paving the Way for Precision OncologyInsideScientific
Experts present a cell-free plasma biobank and describe the role of longitudinal plasma samples for cancer research, disease monitoring, and biomarker development.
Through liquid biopsies, it is now possible to repeatedly and non-invasively interrogate the molecular landscape of solid tumors via a blood draw over the whole treatment course. Until now, liquid biopsies can be used for screening, disease monitoring and prognosis. Circulating tumor DNA (ctDNA) and circulating tumor cells (CTCs) have been the most explored targets in this technology for commercial applications up to the present time.
In collaboration with a continuously expanding oncology network, Indivumed Services has established a unique high-quality cell-free plasma biobank that is exclusively focused on collecting longitudinal whole blood samples from cancer patients. This allows molecular insight by providing quick access to longitudinal plasma from cancer patients that have undergone treatment. ctDNA can then be isolated from longitudinal cell-free plasma to allow for monitoring of disease progression by providing diagnostic and prognostic information, potentially in real time.
Key Topics Include:
- Gain insights into Indivumed Services’ longitudinal plasma collection process
- Understand the advantages and benefits of utilizing longitudinal plasma samples for cancer research
- Explore applications of longitudinal plasma samples for biomarker research and development of companion diagnostics
Fully Characterized, Standardized Human Induced Pluripotent Stem Cell Line an...InsideScientific
In this webinar, experts present a standardized stem cell line and its differentiation into neural cells for disease modeling and assay development.
Reproducible research with human induced pluripotent stem cells (iPSCs) depends on thoroughly characterized and quality-controlled cell lines. In this webinar, Dr. Andrew Gaffney and Dr. Erin Knock from STEMCELL Technologies describe the generation of a standardized induced pluripotent stem cell (iPSC) line. Developed with the upcoming ISSCR Standards Initiative characterization guidelines in mind, this highly characterized line is karyotypically stable, demonstrates trilineage differentiation potential, and expresses undifferentiated cell markers. Further, STEMCELL has developed a highly pure, ready-to-use neural progenitor cell product expressing PAX6 and SOX1 over multiple passages.
Dr. Knock shows how these multipotent cells are suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes. These progenitor cells are the ideal controls for standardizing downstream differentiation protocols, modeling diseases, and assay development.
Key Topics Include:
- Discover how STEMCELL’s induced pluripotent stem cell lines are derived and characterized
- Learn how to differentiate induced pluripotent stem cell lines into all three germ layers
- Explore the features of STEMCELL’s neural progenitor cell product
- Differentiate neural progenitor cells into a variety of neural cell types, including neurons and glia
How to Create CRISPR-Edited T Cells More Efficiently for Tomorrow's Cell Ther...InsideScientific
Ian Foster and Steven Loo-Yong-Kee discuss Artisan Bio's STAR-CRISPR system for optimized gene editing in cell therapy, with a focus on the genetifc modification of T cells for cancer immunotherapy.
Cell therapy is an emerging field with great promise for the treatment of various diseases. One of the most exciting areas of cell therapy is the use of T cells that have been genetically modified to recognize and kill cancer cells. While the use of T cells for cancer immunotherapy has tremendous promise, there is still room for improvement. The efficiency, expansion, and functionality of T cells can be enhanced by genetic modification using the STAR-CRISPR system.
Artisan Bio is a biotechnology company focused on developing a CRISPR-mediated editing platform to improve the efficacy and safety of cell therapy products. In this webinar, we will provide a comprehensive overview of Artisan Bio’s STAR-CRISPR system, which is designed to improve the specificity and efficiency of gene editing for cell therapies. We will explain the system’s key components and how we are using a risk-based approach to optimize and validate the editing platform. The webinar will focus on Artisan Bio’s approach to building T cell OS/APPS through iterative improvements to achieve best-in-class editing capabilities and improved cell health metrics.
Key Topics Include:
- Learn about Artisan Bio’s proprietary high-performance STAR-CRISPR system for improving the specificity and efficiency of gene editing for cell therapies
- Explore Artisan Bio’s risk-based, systems approach to technology development, including how to implement Design of Experiments (DoE) and Quality by Design (QbD) principles to optimize and validate any process
- Case study of the application of QbD to Artisan Bio’s STAR-CRISPR platform to edit T cells for cancer immunotherapy with preliminary data showing improved efficacy, expansion, and functionality
Peripheral and Cerebral Vascular Responses Following High-Intensity Interval ...InsideScientific
Dr. Bert Bond and Max Weston will present an overview on their study investigating the effects high-intensity interval exercise has on cerebrovascular health.
Physical activity reduces the risk of developing cardiovascular diseases (CVD) and dementia. This benefit cannot be explained by changes in traditional CVD risk factors alone, and direct improvements in vascular health are thought to play a key role. However, our understanding of how exercise can be optimized for improvements in blood-vessel health is limited.
High-intensity interval exercise (HIIE) is known to improve peripheral vascular function, and there is a growing interest in the effects of HIIE on cerebrovascular health. However, it is not clear whether the acute improvements in peripheral vascular function following HIIE are also seen in the major blood-vessels of the brain.
In the Bond lab’s study, 30 minutes of HIIE completed at both 75% and 90% V̇O2max improved peripheral vascular function 1 and 3h following exercise in healthy young adults, compared with work-matched continuous moderate-intensity exercise and a sedentary control condition. By contrast, cerebrovascular function was unchanged following all conditions. This is the first study to identify that acute improvements in peripheral vascular function following high-intensity interval exercise are not mirrored by improvements in cerebrovascular function in healthy young adults.
Leveraging Programmable CRISPR-Associated Transposases for Next-Generation Ge...InsideScientific
Dr. Sam Sternberg discusses a novel CRISPR-Cas9 system using programmable, RNA-guided transposase, and highlights its implications for kilobase-scale genome engineering in cell and gene therapies.
The utility of programmable, RNA-guided CRISPR-Cas systems in genome engineering continues to evolve. Nature has afforded scientists novel and diverse gene editing functionality, from nuclease-dependent CRISPR-Cas9 to second-generation base and prime editors that do not produce double-strand breaks.
In this webinar, Dr. Sam Sternberg describes a new CRISPR-Cas9 paradigm relying on nuclease-deficient bacterial transposons that catalyze RNA-guided integration of mobile genetic elements into the genome. The discovery of a fully programmable, RNA-guided transposase lays the foundation for kilobase-scale genome engineering with broad applications for developing cell and gene therapies.
Key Topics Include:
- The basics of first- and second-generation CRISPR-Cas technologies from a scientist at the forefront of their development
- Mechanisms, accommodation, and cell type diversity of CRISPR-Cas programmable transposition
- How transposase factor coordination enables highly specific, genome-wide DNA integration to target sites
- Implications of programmable transposases that obviate the need for DNA double-strand breaks and homologous recombination
Simple Tips to Significantly Improve Rodent Surgical OutcomesInsideScientific
Dr. Marcel Perret-Gentil presents six simple-to-implement techniques to significantly improve surgical outcomes.
You may feel proficient, even confident in performing rodent surgery; however, you may be surprised how simple improvements can have a huge impact to your animal’s recovery and data. The presentation is designed for individuals who have minimal or no rodent surgical skills but is also a great opportunity for those with considerable experience wanting to improve outcomes as well as teach such key principles.
Key Topics Include:
- Improve surgical outcomes that will lessen post-op morbidity and mortality
- Improve data yield after rodent surgery
- Implementation of key principles into a rodent surgical program
Cardiovascular Autonomic Dysfunction in the Post-COVID Landscape: Detection a...InsideScientific
A world-wide spread of the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has triggered a pandemic and generated over 600 million reported cases around the globe. A substantial portion of patients who suffered Corona Virus Disease 2019 (COVID-19) have not recovered completely but continue to experience lingering symptoms for months to years. This novel clinical syndrome has been termed Long COVID or Post-acute Sequalae of COVID-19 (PASC).
Observational studies have indicated that in about one third of cases PASC can be associated with cardiovascular (CV) autonomic dysfunction including postural orthostatic tachycardia syndrome, inappropriate sinus tachycardia, orthostatic hypotension, reflex syncope and microvascular dysfunction. The presence of CV autonomic dysfunction in PASC is important to detect since although frequently overlooked, it may be effectively treated in contrast to many other Long COVID-related symptoms.
This webinar highlights CV dysautonomia as a specific sequalae of acute COVID-19 and guides the audience in the diagnostic work-up of PASC patients with suspected cardiovascular complications.
Creating Better Gene-Edited Cell Lines with the FAST-HDR SystemInsideScientific
Cell lines are the core of biological research. Scientists need cell lines for drug development, basic biology research, safety testing, and biologic therapeutic production. Since the 1980s, genetic manipulation has allowed researchers to tailor cell lines to the experiment or production purpose. Over time, the requirements for these cell lies have risen. In many cases, the cells require multiple genetic edits and must produce data that passes FDA. Moreover, the current funding environment often requires rapid delivery of these cells so scientists can produce data to support further budget and/or investment. This is particularly acute for knock-in cell lines. Current technologies may take months to complete a cell line, allow a limited number of edits, and often have off-target effects that are not suitable for FDA filings. ExpressCells uses its patented FAST-HDR plasmid--along with CRISPR, to address these problems. The FAST-HDR process can precisely knock-in multiple genes (while supporting other types of genetic modifications), ensure precise placement of these edits, and deliver them months faster than competing technologies.
This webinar will discuss the basis of the FAST-HDR technology and illustrate several uses. The first part is a presentation by Oscar Perez-Leal, MD, the inventor of the technology. Oscar will discuss the problems he faced as a researcher and how FAST-HDR was designed to address them. He will outline the details of the technology, the history of its development, and several examples where he used FAST-HDR. The second part is a conversation with Jon Weidanz, PhD. Jon will outline the challenges he faced at AbeXXa and how he selected a FAST-HDR custom cell line for his project. He'll outline the learnings from using this cell line, some of which were unexpected, but valuable to future development.
By attending this program, attendees will:
- Understand the current challenges in creating custom gene-edited cell lines
- Know the technology underlying the FAST-HDR gene-editing system, including its use with CRISPR
- Be able to describe the advantages of the FAST-HDR system
- Learn about several case studies using gene-edited cell lines
Functional Recovery of the Musculoskeletal System Following Injury - Leveragi...InsideScientific
Watch Dr. Sarah Greising discuss the current pathophysiologic understanding of the skeletal muscle remaining following traumatic musculoskeletal injuries.
Volumetric muscle loss (VML) injuries result in the abrupt loss of skeletal muscle fibers, causing chronic functional disability in part due to limited muscle regeneration and vast co-morbidities. With a focus on clinically relevant outcome measurements for skeletal muscle function in both small and large animal models of VML injury, this webinar presents various near-term interventions for the restoration of tissue function following complex injuries. Interventions evaluated focus on regenerative rehabilitation approaches using regenerative pharmaceuticals to correct underlying muscle pathophysiology.
Designing Causal Inference Studies Using Real-World DataInsideScientific
In this webinar, experts provide an overview of causal inference, along with step-by-step guidance to designing these studies using real-world healthcare data.
Causal inference is used to answer cause and effect research questions and yield estimates of effect. Causal study design considerations and statistical methods address the effects of confounding variables and other potential biases and allow researchers to answer questions such as, “Does treatment A produce better patient outcomes compared to Treatment B?”
Causal study interpretations have traditionally been restricted to randomized controlled trials; however, causal inference applied to observational healthcare data is growing in importance, driven by the need for generalizable and rapidly delivered real-world evidence to inform regulatory, payer, and patient/provider decision making. The application of causal inference methods leads to stronger and more powerful evidence. When these techniques are applied to observational data, the results generated are both from and for the real world.
Presenters walk through several real-world case studies including the PCORI-funded BESTMED study and a collaborative study with a prominent pharmacy payer.
Social Media Data: Opportunities and Insights for Clinical ResearchInsideScientific
Many new data are emerging in recent years - real time data is collected through digital health technologies, including apps and wearables, monitoring data, social media data, public datasets, and patient organization data, in addition to primary and secondary datasets.
Real life data are highly informative and can be used to address a range of challenges throughout the product life cycle. Data from social media can generate valuable insights as patients often gather in digital communities to get answers and share their experiences. Conversations on social networks merit special consideration as they can have real world influence over treatment management decisions.
Social media data can reveal the motivations that impact patient healthcare decisions and behaviors through each stage of the care pathway. These data provide both the patient and caregiver perspectives at the same time. For this reason, conversations on social networks offer an opportunity to deepen our understanding on:
- The fears and hopes associated with patient treatments
- Daily needs and difficulties patients are facing in managing their disease
- The impact of disease on patient health related quality of life
- Identification in real life of the stages of the care pathway and patient perceptions
- Reactions to health policies
Watch this webinar for insights on how to collect, use, analyze, and interpret social media data in different contexts. Our experts share knowledge from over fifteen years of successfully developing and adapting algorithms to treat this kind of data.
We Are More Than What We Eat Dietary Interventions Depend on Sex and Genetic ...InsideScientific
To learn more visit: https://insidescientific.com/webinar/we-are-more-than-what-we-eat-dietary-interventions-depend-on-sex-and-genetic-background/
Despite evidence that sex and genetic background are key factors in the response to diet, most studies of how diet regulates metabolic health and even longevity in mice examine only a single strain and sex.
Using multiple strains and both male and female mice, Dr Lamming's team has found that improvements in metabolic health and in longevity in response to reduced levels of protein or specific amino acids strongly depend on sex and strain. While some phenotypes were conserved across strains and sexes, including increased glucose tolerance and energy expenditure, they observed high variability in adiposity, insulin sensitivity, and circulating hormones. Using a multi-omics approach, they identified mega-clusters of differentially expressed hepatic genes, metabolites, and lipids associated with each phenotype, gaining new insight into role of the energy balance hormone FG21 in the response to protein restriction.
Antibody Discovery by Single B Cell Screening on Beacon®InsideScientific
Amy Sheng, PhD provides an overview of antibody screening platforms and presents applications and case studies using the Beacon® platform for antibody discovery.
Single B cell screening is a powerful and efficient strategy for generating antigen-specific monoclonal antibodies. Distinguished with fluorescence-activated B cell sorting, the Beacon® platform is based on plasma cell screening, making it easier to obtain antibody genes.
The Beacon® single-cell optofluidic system combines a unique optoelectro positioning (OEP) technology with novel microfluidic technology. It can be used to accurately select single cells on a chip, perform multiple single-cell assays, and export target cells based on specific results. The Beacon® optofluidic platform preserves the diversity of B cells, generating high-quality positive hits at an early stage of discovery and avoiding the loss of “good clones”.
Key Topics Include:
- B cell differentiation and development
- Pros and cons of mainstream antibody screening platforms
- Mechanisms, applications, and case studies using the Beacon® platform for antibody screening
- Sino Biological’s capacity using the Beacon® platform
Experimental Design Considerations to Optimize Chronic Cardiovascular Telemet...InsideScientific
Phil Griffiths, PhD, presents a summary of chronic cardiovascular telemetry studies and considerations for experimental design.
Ensuring you collect the best and most physiologically accurate data from your chronic telemetry experiments requires careful planning and experimental design. This webinar will give an insight into the practical aspects of designing chronic animal experiments to set you on the best path for success. The benefits of chronic studies, how to select the most appropriate sample size for your study, some basic tips and tricks for data acquisition and handling, and how to ensure high animal welfare are discussed.
Key Topics Include:
- What are the benefits of chronic over acute studies?
- How to decide the best sample sizes and the length of experiments?
- Basic tips for data acquisition and handling
- How to maintain high animal welfare standards
Strategic Approaches to Age-Related Metabolic Insufficiency and Transition in...InsideScientific
In this webinar, Dr. Dennis Turner delves into dementia syndrome, the metabolic changes that occur, and the importance of proper physiological monitoring of animal models.
Brain metabolism transforms with normal aging, and transient, dynamic metabolic insufficiency may underlie critical progression from aging into dementia syndrome and Alzheimer’s disease (AD). Age-related brain metabolism balances vascular-related substrate supply and transport mechanisms into extracellular space to neurons with cellular metabolic needs and utilization. Dynamic metabolic insufficiency can occur when there is intermittent supply-demand mismatch.
Adequacy of neurovascular coupling to provide sufficient cerebral blood flow (CBF) to meet neuronal demand in vivo in a mouse AD model, compared to aged controls were studied. Dr. Turner’s lab analyzed the response to maximal neuronal metabolic demands, spreading depression and anoxia, using imaging, CBF measurements, and oxygen and glucose levels. These in vivo studies require human-similar anesthesia conditions, through monitoring temperature, blood pressure/pulse oximetry, and respiration, to maintain homeostasis. The lab confirmed abnormal neurovascular coupling in a mouse model of AD in response to these metabolic challenges, showing disruption much earlier in dementia than in equivalently aged individuals. Chronic metabolic treatments could influence dementia syndrome progression.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...
Implantable Infusion Pumps: Insights For Your Next Animal Dosing Study
1. Implantable Infusion Pumps:
Insights For Your Next Animal Dosing Study
A webinar for scientists interested in the use of implantable pumps as an
alternative to repetitive injections when administering compounds to
laboratory animals. Presenter, José Gadea, clarifies misconceptions
surrounding the use of implantable pumps and presents
facts supporting their value in preclinical research.
2. InsideScientific is an online educational environment
designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information
regarding innovative technologies, protocols, research
tools and laboratory services.
JOIN FOR FREE AT WWW.INSIDESCIENTIFIC.COM
4. Thank you to our event sponsor
Toll Free: 877-922-5938 (U.S. & Canada)
Phone: 408-253-8574
Fax: 408-865-1406
E-mail: alzet@durect.com
To place orders online from North America,
click here
For international ordering information,
click here
5. 1. Overview of Laboratory Animal Dosing Options
2. Brief History of Implantable Pumps
3. Features, Benefits and Applications of Implantable Pumps
4. Helpful Tips for Planning Studies
5. Myths About Implantable Pumps
6. Q&A
What are we going to cover today?
9. Laboratory Animal Dosing Options
Advantages
• Perceived as simple
• Inexpensive
Limitations
• Technical difficulty
• High level of animal stress
(tissue trauma, mortality)
• Inconsistent dosing
• Negative influence on
experimental results
Injections
Implantable
Pumps
Ambulatory
Pumps
Tethered
Infusion
Pellets
Food &
Water
10. Laboratory Animal Dosing Options
Advantages
• Quick procedure
• Readily available for some
drugs
Limitations
• Not available for many
drugs
• Require customization
• Costly
• Inconsistent dosing (not
continuous)
Injections
Implantable
Pumps
Ambulatory
Pumps
Tethered
Infusion
Gavage
Food &
Water
11. Laboratory Animal Dosing Options
Advantages
• Dose control
• High volumes
• Stability and solubility
Limitations
• Costly equipment
• Require maintenance
• Potential risk of catheter
clotting or disconnection
• Increased risk of infection
• Animal stress
• Restrict animal movement
• Prevents social housing
Injections
Implantable
Pumps
Ambulatory
Pumps
Pellets
Gavage
Food &
Water
12. Laboratory Animal Dosing Options
Advantages
• Dose control
• High volumes
• Stability and solubility
Limitations
• Costly equipment
• Require maintenance
• Animal stress
• Restrict animal movement
• Require a jacket
• Potential risk of catheter
clotting or disconnection
• Increased risk of infection
Injections
Implantable
Pumps
Tethered
Infusion
Pellets
Gavage
Food &
Water
13. Implantable Infusion Pumps
Limitations
• Surgery required
• Formulation as
solutions
• Learning curve
(programming)
• Cost depending on
alternatives
Benefits
• Automatic dosing
• Dose control
• Improved efficacy
• Reduced side effects
• Animal welfare
• Better data
14. A Brief History
• Developed by ALZA Corporation and
commercialized in 1977
• DURECT Corp. acquired ALZET line in 2000
(Cupertino, California, USA)
• Specialty pharmaceutical with proprietary
drug delivery technologies
• Manufactures and distributes ALZET® pumps
worldwide
• Authorized distributor of iPRECIO® pumps in
North America since Oct. 2012
• Developed by Primetech Corporation (Tokyo,
Japan)
• Manufacturer and distributor of medical and
analytical science products
• Manufacturer of iPRECIO® Programmable
Pumps
• iPRECIO SMP-101L: March 2007
• iPRECIO SMP-200: July 2009
• iPRECIO SMP-300: April 2014
15. ALZET Osmotic Pumps
• Miniature infusion devices for
continuous dosing of
unrestrained lab animals
• Chronic delivery at controlled
rates
• Continuous delivery of a wide
range of agents
• Short half-life compounds
• Nearly 17,000 publications
16. • Small size: mice & young rats
• Reliable: 16,500+ pubs (40 yrs)
• Fully implantable – no stress
• Convenient alternative to injections
• Chronic delivery
• Simple & easy to use
• Cost-effective
• Continuous administration
ALZET Pumps: Key Features and Benefits
17. Benefits of Continuous Administration
• Automatic and undisturbed dosing
• Stable drug levels
• Improved therapeutic efficacy
• Reduced side effects
• Drug savings
23. ALZET Pump Selection
• Animal size
• Route of administration
• Duration of infusion
• Drug solubility
• Choose the smallest pump possible taking
into account agent solubility
• Solubility issues: choose a pump with larger
reservoir volume or faster flow rate
ALZET Tip: Use the online interactive pump selector tool
24. ALZET Pump: Agent Selection
Broad agent compatibility:
• Peptides, hormones, nanoparticles, steroids,
radioisotopes, chemotherapeutic agents, growth
factors, antibiotics, and pharmaceuticals
Molecular size:
• Delivery is independent of the compound’s
molecular weight, physical conformation, or
chemical properties
MYTH - ALZET pumps can’t deliver large size compounds
FACT - Molecules of any molecular weight can be delivered
25. ALZET Pump: Agent Requirements
Stability:
• Compound must be stable at 37o C for study
duration
Solubility:
• Compound must remain in solution for duration
of the study
• Precipitate can block the exit port of the pump,
catheter or cannula tip
31. ALZET Pump: Viscous Solutions
MYTH - ALZET pumps can’t deliver viscous solutions
FACT - ALZET pumps are capable of delivering
homogeneous solutions with a viscosity of less than
100,000 cP or mPa s.
32. ALZET Pump: Drug Formulation
MYTH – Using nominal specifications for dose calculations
FACT – Use actual pumping rate and fill volume listed on the
specifications sheet for each lot of pumps when making dose
calculations Model 2002 (Lot #10196-08)
Nominal Actual
Release Rate 0.5 ml/hr 0.53 ml/hr
Fill Volume 200 ml 216 ml
Duration 14 days 16.1 days
33. ALZET Pump: Filling
• Use aseptic technique
• Weigh pump and flow moderator before filling
• Use the filling tube provided
• Fill the pumps with the curved end down
• Fill with the flow moderator removed
• A small amount of backpressure is normal
Expert tip: If you experience too much pressure, angle the needle slightly to
allow air to escape, or insert and remove the flow moderator a few times to
widen the opening.
34. ALZET Pump: Priming
Priming is essential when:
• Immediate pumping is required
• A catheter is used with the pump
• A viscous solution is delivered
• The drug solution may have acute toxic effects
Priming ensures that the pumps deliver at their specified
pumping rate at time of implantation. To prime, place
the filled ALZET pumps into an aqueous solution at 37 C
for a specified time (3-60 hours depending on pump
model).
36. ALZET Pump: SC Implantation Procedure
The usual site for SC implantation in rats and mice is on the back, slightly
posterior to the scapulae.
Procedure:
• Anesthetize and shave the animal
• Make a mid-scapular incision suitable for the pump
• Create a subcutaneous pocket by blunt dissection using a hemostat
• Insert the pump into the pocket, delivery portal first
• Close the incision with wound clips
MYTH - ALZET pump implantation is too difficult
FACT – The SC implantation procedure can be performed in under a minute
37. Minimum Size for Implantation of ALZET Pumps
ALZET Models
1003D, 1007D, 1002,
1004
2001D, 2001, 2002,
2004, 2006
2ML1, 2ML2, 2ML4
MICE
Subcutaneous 10 g 20 g N/A
Intraperitoneal 20 g N/A N/A
RATS
Subcutaneous 10 g 20 g 150 g
Intraperitoneal 20 g 150 g 300 g
Note: Estimates based on experience with Sprague Dawley rats and Swiss Webster mice.
38. ALZET Pump: Implantation
MYTH - ALZET pumps restrict animal movement
FACT – Rodents have very loose skin on the back. ALZET
pumps are used in mice and rats that are used in various
behavioral tests (I.e., open field, rotarod, Morris water task,
swim test, etc.)
39. Intravenous
Brain cannulation
Targeted Delivery
• Blood vessels
• Central Nervous System
– Cerebral ventricles, brain
tissue, Spinal Cord
• Peripheral nerves
• Various organs and tissues
– Tumor, bone, eye, ear, muscle,
wound
ALZET Pump: Routes of Administration
40. ALZET Pump: Catheter Applications
1. To delay drug delivery
• Pump filled with drug
• Catheter filled with vehicle
2. To reduce drug waste
• Pump filled with vehicle
• Catheter filled with drug
3. To deliver incompatible solvents
41. 1. Measurement of plasma levels
2. Measurement of residual volume
3. In vitro release rate testing
ALZET Pump: Verifying Delivery
MYTH – Weighing pumps at the end of the study to confirm delivery
FACT – The weight of a partially empty pump or cutting a pump is not a reliable
means of verifying pump performance
42. iPRECIO Programmable Pump
24.8 mm
15.0 mm
H: 7.2 mm
Weight: 3.3 g
19.2 mm
H: 9.7 mm
Weight: 7.9 g
38.7 mm
SMP-300 SMP-200
45. • In vivo refilling via percutaneous access
• Eliminate multiple surgeries
• Increasing dose studies
• Multiple drugs
• Poor drug stability/solubility
iPRECIO Pump: Refillable
MYTH – iPRECIO pumps can be reused
FACT – iPRECIO pumps are refillable, but not reusable
46. • Full control and flexibility over dosing
protocols
• Set your own infusion protocol: start/stop
time, flow rate, duration, etc
• Download infusion protocol to the pump via
communication device
• SMP-200: IR communication
• SMP-300: wireless communication
iPRECIO Pump: Programmable
48. • Accurate and reliable “Rotary Finger” mechanism
(Pulse micro-motor)
• Microprocessor and associated circuitry for pump
control (CPU, memory)
• Power source (battery)
• Fluidics (Reservoir, catheter, filling port)
• Pump programming and communication interface
(software and base station)
iPRECIO Pump: Key Components
50. Antenna: 50 mm titanium wire (0.1 mm OD) inside PU tubing (0.4mm OD)
Top ViewBottom View
iPRECIO SMP-300
51. iPRECIO Pump: Mechanism of Operation
Patented "Rotary Finger" mechanism
• A micro-motor slowly revolves in a clockwise direction
turning the cam with its four projections.
• In each quarter rotation, a single cam projection
sequentially pushes up each of the seven finger pins.
• This continuous cycle compresses the liquid filled tube,
creating a peristaltic-like movement of the fluid.
• As the solution moves through the tube, it is expelled
from the pump reservoir into the test subject.
Rotary Finger Mechanism
• Accuracy: +/- 5%
• Each pump calibrated at factory
52. iPRECIO® Management System IMS-200
• Data communication Device UCD-200
• USB cable
• iPRECIO® Software Installation CD
• iPRECIO® Users Manual
• (2) AAA cell batteries
iPRECIO® Management System
iPRECIO® Management System IMS-300
• Data communication Device UCD 300
• LAN cable
• iPRECIO® Software Installation CD
• iPRECIO® Users Manual
53. iPRECIO Programming (SMP-200)
1. Enter study details
• Name/ID
• Date
• User
• Animal: species/strain/age
• Route
• Duration
• Compound name and
concentration
• Groups / # of animals
54. iPRECIO Programming (SMP-200)
2. Enter group details
• Est. min and max animal
weight
• Drug concentration
• Select infusion mode/group
• Instant vs. delayed
• Select flow rate mode/group
• Constant vs. variable
55. iPRECIO Programming (SMP-200)
3. Enter animal details
• Individual animals per group
• Animal ID
• Weight
• Sex
• Set infusion mode
• Start date/time
• End date/time
• Detect pump
56. iPRECIO Programming (SMP-200)
4. Pump information
• Individual pump programming
• Step 1-10
– Dose
– Duration
– Start/end time
– Flow rate
• Program pump
• Print report
57. iPRECIO Programming (SMP-300)
Monitor function:
Allows the user to follow the
infusion profile in detail. Refill
dates/exchange dates and
alarms are also managed and
displayed here.
58. iPRECIO Pump: Key Specs Comparison
SMP-300 SMP-200
Size [L] x [W] x [H]
(Weight/volume)
24.8 x 15.0 x 7.2 mm
(3.3 g / 2.15 cc)
38.7 x 19.2 x 9.7mm
(7.9 g / 7.20 cc)
Communication Wireless Infrared
Animal Species Mice or larger Rats or larger
Animal Size Suggested: 25 g (Min. 22 g) Suggested: 230 g (Min. 160 g)
Reservoir Volume 130 μL 900 μL
Flow Rate
(Setting Resolution)
0.1 – 10.0 μL/hr
(0.1 μL/hr)
0.1 – 30.0 μL/hr
(0.1 μL/hr)
Duration Up to 47 days Up to 6 months
59. Battery Life: iPRECIO SMP-200
Flow Rate
Continuous Infusion
Total Infusion
Volume(Time) (hours)
1.0 μL/hr 6 months 4,328 hr 4.3 mL
8.5 μL/hr 1 month 669 hr 5.6 mL
19.0 μL/hr 1.8 weeks 307 hr 5.8 mL
30.0 μL/hr 1 week 196 hr 5.8 mL
60. Comm.
Interval Every Minute Every 2 hrs Every 6 hrs Every 24 hrs None
Flow Rate
(ul/hr)
Time (days) Time (days) Time (days) Time (days) Time (days)
0.1 16 37 42 45 47
1.0 14 28 31 32 33
5.0 10 15 15 16 16
10.0 7 9 9 9 9.0
Battery Life: iPRECIO SMP-300
61. iPRECIO Pump: Vehicle Selection
Not Compatible Vehicles
• Acids (< pH 1.8)
• Bases (> pH 14)
• Benzyl-alcohol (>10%)
• Oils (Corn, Mineral, Sesame)
• DMSO (100%)
• DMSO: ethanol (50:50)
• DMSO:PEG 400/300/200 (50:50)
• Ethyl Oleate
• Solutol® (>30%)
Short Term Use Vehicles
• PEG 300/400 (100%) < 45 days
• Cremophor EL (25%) < 30 days
• PEG 400/PG/Water (30:50:20) < 30 days
Viscous solutions
• Viscosity up to 20 cP has been evaluated
• Higher viscosity not recommended
• Difficult to aspirate through 27G needle
62. iPRECIO Pump: Agents
Angiotensin II
Ang II antagonists
Cardiovascular drugs
Corticotropin-rel. hormone
Dobutamin
Epinephrine
Estradiol
GPR54 Agonist
Nicotine
Olanzapine
Pentobarbital
Reproductive hormones
Serotonin (5-HT)
Valsartan
Verapamil
Vitamin B12
67. Complete Dosing Solution
Species Mice & Larger Mice & Larger
Dosing Continuous Continuous, Variable, Bolus
Durations 1-42 days <45 days (SMP-300); <6 months (SMP-200)
Refillable No Yes
Infusion Volumes Small Small & large
Compound Requirement Soluble & Stable Low solubility and stability
Accuracy > 10% > 5%
Infusion Rates Fixed Programmable
Cost Cost-effective High budget studies
68. Contact for Help and Resources
Toll Free: 877-922-5938 (U.S. & Canada)
Phone: 408-253-8574
Fax: 408-865-1406
E-mail: alzet@durect.com
To place orders online from North America,
click here
For international ordering information,
click here
69. Thank You!
José R. Gadea
Sr. Product Marketing Manager,
DURECT Corporation
Contact Information:
jose.gadea@durect.com
Phone: 800-692-2990
For additional information on the solutions
presented in this webinar please visit
www.alzet.com