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Improving Rodent
Cardiovascular Research
Outcomes with Integrated
Surgical Monitoring
Sponsored by:
Improving Rodent Cardiovascular Research Outcomes
with Integrated Surgical Monitoring
1. Attaining ‘Normal’ Cardiac Function for PV Analysis (Dr. James Clark)
• Parameters to Monitor & How
• The importance of Fluid-Support
• Effects of Anaesthesia and Cooling
• How Temperature can Effect Infarct Size
2. Demonstration: Integrated Monitoring with the MouseMonitor™ S (Graham Sattler)
• System Operation, File Recording and Playback
• Screenshots for Collaboration
• System Integration Components and Configuration
• Presets and Flexibility
Sponsored by:
InsideScientific is an online educational environment
designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information
regarding innovative technologies, protocols, research
tools and laboratory services.
Attaining ‘Normal’ Cardiac
Function For PV Analysis
Dr. James E. Clark
Senior Lecturer in Applied Physiology
British Heart Foundation Centre of Excellence,
Cardiovascular Division,
Faculty of Life Sciences and Medicine,
King’s College London
0 10 20 30 40 50
0
20
40
60
80
100
120
Volume (µl)
LVPressure(mmHg)
Complicated Surgery occupies the surgeon
Myocardial infarction
Abdominal aortic
banding
PV Loop acquisition
Myocardial infarction
Abdominal aortic
banding
PV Loop acquisition
Complicated Surgery occupies the surgeon
EFFECTIVE MONITORING IS KEY!
Parameters to Monitor
• Temperature
• ECG
• Respiration
• Oxygen Saturation
• Blood Pressure
• Respiratory Gasses (ETO2 and ETCO2)
• Blood Flow (peripheral)
ESSENTIALDESIRABLEOPTIONAL
• Operating table and
microscope
• Homeothermic platform
• Physiological Monitoring
• PV Catheter and data
acquisition system
• Suitable anaesthetic
• Ventilation
• Surgical instruments
Essentials for
Murine PV Surgery
Effective Surgical Monitoring
Data Acquisition Temperature ControlElectrocardiogram
Isolated Carotid Artery PV Catheter Insertion
Blood-free Surgical Field & Fluid Support
• Maintain ‘normal blood volume’
during any measurement
– Proper hydration
– Fluid support
• Fluid support can be
administered I.V. or I.P.
– Saline
– 1-3% BSA
– Hydroxyethyl starch
• Care should be taken not to
over-hydrate
From Zuurbier et al, AJP (282), 2002
The Importance
of Fluid Support
Haes (0.2ml/10g/hr)
Saline (0.5ml/10g/hr)
0 10 20 30
0
20
40
60
80
100
Volume (µl)
Pressure(mmHg)
Abdominal IVC occlusion
Fluid Support Maintains Blood Pressure
Resulting PV Loop measures
Procedure Room at BHF Centre
Purpose-built Operating Table
Integrated heat mat and rectal
(T-type) rectal thermister
Yang X et al. Am J Physiol Heart Circ Physiol 1999;277:H1967-H1974
K e t/M e d 2 % IS O 2 % IS O
0
2 0 0
4 0 0
6 0 0
0
5 0
1 0 0
1 5 0
HR(bpm)
EF(%)&SP(mmHg)
HR E F % S P (m m H g )
In tu b a te d
*
*
*
**
Clark et al. (unpublished data)
Anaesthetics and Cardiac Function
Picard et al, F1000Research (3), 2014
Effect of the Depth of Anaesthesia
There are considerable
differences between
INDUCTION and
MAINTENANCE-levels of
anaesthesia on function.
Even small changes in temperature can effect function
Pressure(mmhg)
0
20
40
60
80
100
Baseline Cooling Re-warmed
Clark et al. (unpublished data)
Affect of Cooling on Developed Pressure
Baseline Heater Turned Off (7.5 minutes) Re-warmed (200 ul 42°C saline I.P.)
Temperature Monitoring DURING Procedures
Pressure(mmhg)
0
20
40
60
80
Unless homeostasis is maintained data will ‘drift’
Clark et al. (unpublished data)
20 25 30 35 40
0
50
100
150
Core Temperature (°C)
SystolicPressure(mmHg)
20 25 30 35 40
0
10
20
30
40
Core Temperature (°C)
StrokeVolume(µl)
The Importance of Temperature Monitoring
C57bl/6j mice subjected to cooling/re-warming protocol Clark et al. (unpublished data)
(N=5) (N=5)
0
25
50
75
100
37˚C
32˚C
24˚C
20˚C
RVU
LVPressure(mmHg)
Clark et al. (unpublished data)
Core Temperature
The Importance of Temperature Monitoring
Apical PV Access 0 200 400 600 800
32
34
36
38
40
500
550
600
650
Time (sec)
CoreTemperature
(°C)
HR(bpm)
Temp
HR
Thoracotomy
39-40°C saline-soaked
gauze to avoid
evaporative heat loss
Clark et al. (unpublished data)
Maintenance of temperature throughout protocol
Clark & Marber Exp.Physiol. (2013)
Murine Myocardial Ischaemia-Reperfusion
Suture snare system
Clark et al. Journal of Pharmacological and Toxicological Methods, Vol. 59, No. 2, 03.2009, p. 94 - 99.
Murine Myocardial Ischaemia-Reperfusion
Hanging weight system
Murine Myocardial Ischaemia-Reperfusion
Balloon in place
Balloon inflated
Clark & Marber Exp.Physiol. (2013)
Eckle et al. Am J Physiol Heart Circ Physiol 291: H2533–H2540, 2006
Infarct size is dependent on temperature
Sutherland et al. Clin and Exp. Pharmacol. and Physiol. (2003) 30, 867–878
Tissier R et al. Cardiovasc Res. (2012) 94(2):217-25
Infarct size is dependent on temperature
Cardiac temperature (°C) during a 30 min CAO in rabbits Cardiac temperature (°C) during a 60 min CAO in sheep
Surgically-mediated Changes in ECG
• ECG -- limb lead and
augmented leads
• HR
• Breathing rate
• Core temperature
• Blood oxygenation
(with SpO2 module)
• Data recording
– On tablet and/or
– Analogue output
MouseMonitor™ S
Preliminary Results
 Stable monitoring
platform
 No electrical interference
with PV acquisition
 Reliable ECG and data
recording using built-in
pads or needle electrodes
 Temperature control
0 10 20 30 40 50
0
20
40
60
80
100
120
Volume (µl)
LVPressure(mmHg)
A Demonstration: Integrated
Surgical Monitoring with the
MouseMonitor™ S
Graham Sattler
Product Development
Manager,
Indus Instruments
1. PV Loop Theory
2. PV Loop Applications
3. PV Loop System Setup Tips & Best Practices
4. PV Loop Surgical Preparation Overview
5. PV Loop Signal Optimization Tips & Best Practices
6. PV Loop Procedure Documentation
PV Loop Webinar Series so far…
1. PV Loop Theory
2. PV Loop Applications
3. PV Loop System Setup Tips & Best Practices
4. PV Loop Surgical Preparation Overview
5. PV Loop Signal Optimization Tips & Best Practices
6. PV Loop Procedure Documentation
7. Surgical Procedure Outcome Evaluation
PV Loop Webinar Series so far…
Capability
• Responsive Heating
• Surgical Monitoring
– Heart Rate
– Core Temperature
– ECG (6-Lead)
– Mouse & Rat
Electrodes
• Easy Documentation
View the following demonstration
videos online…
1. System Operation
2. Standalone File
Recording & Playback
3. Capturing Screenshots
for Collaboration
Example Screenshot #1
Example Screenshot #2
Low Latency Sync BNC Cable External Grounding Cable
System Integration Components
View the following demonstration
videos online…
1. System Integration
Configuration
2. Presets & Flexibility
Thank You!
For additional information on Surgical
Monitoring best-practices and solutions for
preclinical research please visit:
http://www.indusinstruments.com
NEXT WEBINAR: Dr. Navin Kapur, Assistant Professor and Assistant
Director of the MCRI at Tufts, will discuss how PV loop data can translate
from mouse to man and provide a confident approach to evaluating drug
studies, device validation and treatments outcomes.
WORKSHOP:

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Improving Rodent Cardiovascular Research Outcomes with Integrated Surgical Monitoring

  • 1. Improving Rodent Cardiovascular Research Outcomes with Integrated Surgical Monitoring Sponsored by:
  • 2. Improving Rodent Cardiovascular Research Outcomes with Integrated Surgical Monitoring 1. Attaining ‘Normal’ Cardiac Function for PV Analysis (Dr. James Clark) • Parameters to Monitor & How • The importance of Fluid-Support • Effects of Anaesthesia and Cooling • How Temperature can Effect Infarct Size 2. Demonstration: Integrated Monitoring with the MouseMonitor™ S (Graham Sattler) • System Operation, File Recording and Playback • Screenshots for Collaboration • System Integration Components and Configuration • Presets and Flexibility Sponsored by:
  • 3. InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools and laboratory services.
  • 4. Attaining ‘Normal’ Cardiac Function For PV Analysis Dr. James E. Clark Senior Lecturer in Applied Physiology British Heart Foundation Centre of Excellence, Cardiovascular Division, Faculty of Life Sciences and Medicine, King’s College London 0 10 20 30 40 50 0 20 40 60 80 100 120 Volume (µl) LVPressure(mmHg)
  • 5. Complicated Surgery occupies the surgeon Myocardial infarction Abdominal aortic banding PV Loop acquisition
  • 6. Myocardial infarction Abdominal aortic banding PV Loop acquisition Complicated Surgery occupies the surgeon EFFECTIVE MONITORING IS KEY!
  • 7. Parameters to Monitor • Temperature • ECG • Respiration • Oxygen Saturation • Blood Pressure • Respiratory Gasses (ETO2 and ETCO2) • Blood Flow (peripheral) ESSENTIALDESIRABLEOPTIONAL
  • 8. • Operating table and microscope • Homeothermic platform • Physiological Monitoring • PV Catheter and data acquisition system • Suitable anaesthetic • Ventilation • Surgical instruments Essentials for Murine PV Surgery
  • 9. Effective Surgical Monitoring Data Acquisition Temperature ControlElectrocardiogram
  • 10. Isolated Carotid Artery PV Catheter Insertion Blood-free Surgical Field & Fluid Support
  • 11. • Maintain ‘normal blood volume’ during any measurement – Proper hydration – Fluid support • Fluid support can be administered I.V. or I.P. – Saline – 1-3% BSA – Hydroxyethyl starch • Care should be taken not to over-hydrate From Zuurbier et al, AJP (282), 2002 The Importance of Fluid Support Haes (0.2ml/10g/hr) Saline (0.5ml/10g/hr)
  • 12. 0 10 20 30 0 20 40 60 80 100 Volume (µl) Pressure(mmHg) Abdominal IVC occlusion Fluid Support Maintains Blood Pressure Resulting PV Loop measures
  • 13. Procedure Room at BHF Centre
  • 14. Purpose-built Operating Table Integrated heat mat and rectal (T-type) rectal thermister
  • 15. Yang X et al. Am J Physiol Heart Circ Physiol 1999;277:H1967-H1974 K e t/M e d 2 % IS O 2 % IS O 0 2 0 0 4 0 0 6 0 0 0 5 0 1 0 0 1 5 0 HR(bpm) EF(%)&SP(mmHg) HR E F % S P (m m H g ) In tu b a te d * * * ** Clark et al. (unpublished data) Anaesthetics and Cardiac Function
  • 16. Picard et al, F1000Research (3), 2014 Effect of the Depth of Anaesthesia There are considerable differences between INDUCTION and MAINTENANCE-levels of anaesthesia on function.
  • 17. Even small changes in temperature can effect function Pressure(mmhg) 0 20 40 60 80 100 Baseline Cooling Re-warmed Clark et al. (unpublished data) Affect of Cooling on Developed Pressure
  • 18. Baseline Heater Turned Off (7.5 minutes) Re-warmed (200 ul 42°C saline I.P.) Temperature Monitoring DURING Procedures Pressure(mmhg) 0 20 40 60 80 Unless homeostasis is maintained data will ‘drift’ Clark et al. (unpublished data)
  • 19. 20 25 30 35 40 0 50 100 150 Core Temperature (°C) SystolicPressure(mmHg) 20 25 30 35 40 0 10 20 30 40 Core Temperature (°C) StrokeVolume(µl) The Importance of Temperature Monitoring C57bl/6j mice subjected to cooling/re-warming protocol Clark et al. (unpublished data) (N=5) (N=5)
  • 20. 0 25 50 75 100 37˚C 32˚C 24˚C 20˚C RVU LVPressure(mmHg) Clark et al. (unpublished data) Core Temperature The Importance of Temperature Monitoring
  • 21.
  • 22. Apical PV Access 0 200 400 600 800 32 34 36 38 40 500 550 600 650 Time (sec) CoreTemperature (°C) HR(bpm) Temp HR Thoracotomy 39-40°C saline-soaked gauze to avoid evaporative heat loss Clark et al. (unpublished data) Maintenance of temperature throughout protocol
  • 23. Clark & Marber Exp.Physiol. (2013) Murine Myocardial Ischaemia-Reperfusion Suture snare system
  • 24. Clark et al. Journal of Pharmacological and Toxicological Methods, Vol. 59, No. 2, 03.2009, p. 94 - 99. Murine Myocardial Ischaemia-Reperfusion Hanging weight system
  • 25. Murine Myocardial Ischaemia-Reperfusion Balloon in place Balloon inflated Clark & Marber Exp.Physiol. (2013)
  • 26. Eckle et al. Am J Physiol Heart Circ Physiol 291: H2533–H2540, 2006 Infarct size is dependent on temperature Sutherland et al. Clin and Exp. Pharmacol. and Physiol. (2003) 30, 867–878
  • 27. Tissier R et al. Cardiovasc Res. (2012) 94(2):217-25 Infarct size is dependent on temperature Cardiac temperature (°C) during a 30 min CAO in rabbits Cardiac temperature (°C) during a 60 min CAO in sheep
  • 29. • ECG -- limb lead and augmented leads • HR • Breathing rate • Core temperature • Blood oxygenation (with SpO2 module) • Data recording – On tablet and/or – Analogue output MouseMonitor™ S
  • 30. Preliminary Results  Stable monitoring platform  No electrical interference with PV acquisition  Reliable ECG and data recording using built-in pads or needle electrodes  Temperature control 0 10 20 30 40 50 0 20 40 60 80 100 120 Volume (µl) LVPressure(mmHg)
  • 31. A Demonstration: Integrated Surgical Monitoring with the MouseMonitor™ S Graham Sattler Product Development Manager, Indus Instruments
  • 32. 1. PV Loop Theory 2. PV Loop Applications 3. PV Loop System Setup Tips & Best Practices 4. PV Loop Surgical Preparation Overview 5. PV Loop Signal Optimization Tips & Best Practices 6. PV Loop Procedure Documentation PV Loop Webinar Series so far…
  • 33. 1. PV Loop Theory 2. PV Loop Applications 3. PV Loop System Setup Tips & Best Practices 4. PV Loop Surgical Preparation Overview 5. PV Loop Signal Optimization Tips & Best Practices 6. PV Loop Procedure Documentation 7. Surgical Procedure Outcome Evaluation PV Loop Webinar Series so far…
  • 34. Capability • Responsive Heating • Surgical Monitoring – Heart Rate – Core Temperature – ECG (6-Lead) – Mouse & Rat Electrodes • Easy Documentation
  • 35. View the following demonstration videos online… 1. System Operation 2. Standalone File Recording & Playback 3. Capturing Screenshots for Collaboration
  • 38. Low Latency Sync BNC Cable External Grounding Cable System Integration Components
  • 39. View the following demonstration videos online… 1. System Integration Configuration 2. Presets & Flexibility
  • 40. Thank You! For additional information on Surgical Monitoring best-practices and solutions for preclinical research please visit: http://www.indusinstruments.com
  • 41. NEXT WEBINAR: Dr. Navin Kapur, Assistant Professor and Assistant Director of the MCRI at Tufts, will discuss how PV loop data can translate from mouse to man and provide a confident approach to evaluating drug studies, device validation and treatments outcomes. WORKSHOP: