1. The proposed treatment for soil contaminated with heavy oils involves a 5-step in situ bioremediation process: biosurfactant washing, bioaugmentation, biostimulation, biocatalysis, and phytoremediation.
2. Bioaugmentation introduces white rot fungi to degrade hydrocarbons using extracellular enzymes and penetrate deep in soil. Biostimulation adds nutrients via biosolids and fertilizer to support microbial growth.
3. Biocatalysis uses enzymes like laccases to catalyze oxidation of contaminants. Phytoremediation employs plants like sunflower and mustard that hyperaccumulate and remove heavy metals or enhance hydrocarbon degradation.
Phytoextraction, also called phytoaccumulation, phytoabsorption, or phytosequestration, refers to the use of plants to absorb, translocate, and store toxic contaminants from soil, sediments, and/or sludge in the root and shoot tissues .
Lead is an extremely difficult soil contaminant to remediate because it is a “soft” Lewis acid that forms strong bonds to both organic and inorganic ligands in soil. For the most part, Pb-contaminated soils are remediated through civil engineering techniques that require the excavation and landfilling of the contaminated soil. Soils that present a leaching hazard in the landfill are either placed in a specially constructed hazardous waste landfill, or treated with stabilizing agents, such as cement, prior to disposal in an industrial landfill.
Phytoextraction, also called phytoaccumulation, phytoabsorption, or phytosequestration, refers to the use of plants to absorb, translocate, and store toxic contaminants from soil, sediments, and/or sludge in the root and shoot tissues .
Lead is an extremely difficult soil contaminant to remediate because it is a “soft” Lewis acid that forms strong bonds to both organic and inorganic ligands in soil. For the most part, Pb-contaminated soils are remediated through civil engineering techniques that require the excavation and landfilling of the contaminated soil. Soils that present a leaching hazard in the landfill are either placed in a specially constructed hazardous waste landfill, or treated with stabilizing agents, such as cement, prior to disposal in an industrial landfill.
Bioremediation is the use of microorganisms (e.g., bacteria, fungi), plants (termed phytoremediation), or biological enzymes to achieve treatment of hazardous waste. Treatment can target a variety of media (wastewater, groundwater, soil/sludge, gas) with several possible objectives (e.g., mineralization of organic compounds, immobilzation of contaminants). In situ bioremediation (ISB) is the application of bioremediation in the subsurface – as compared to ex situ bioremediation, which applies to media readily accessible aboveground (e.g., in treatment cells/soil piles or bioreactors). In situ bioremediation may be applied in the unsaturated/vadoze zone (e.g., bioventing) or in saturated soils and groundwater (Sharma S. 2012).
Bioremediation is the process in which the micro-organisms are used to degrade the pollutants from the environment. Plants and micro-organisms are used to clean up the environment. Bioremediation is carried out by microbes and their metabolisms are used to remove the contaminants. Microbes have the ability to resolve the issue of contaminated ecosystem1. To improve or better living style the degradation of contaminated areas is very important. Importance of the biodegradation is increasing due to the expensiveness of the chemicals. So bioremediation is the best choice. The effluents should be degraded from the environment because they are very dangerous and have a bad impact on human beings. These pollutants sink into the water and cause pollution. These pollutants are treated with the help of microbes in bioremediation process. It is the best method because it is cost effective and eco-friendly. Different techniques of bioremediation are used to convert toxic substances into less toxic substances.
PHYTOREMEDIATION IN ENVT. MANAGEMENT - BIOTECHNOLGY ROLE...KANTHARAJAN GANESAN
It deals with, the various technologies involved in phytoremediation, mechanism, factors and biotechnology interventions for the improvement of remediation process etc...
The USEPA defines biodegradation as a process by which microbial organisms transform or alter (through metabolic or enzymatic action) the structure of chemicals introduced into the environment.
According to the definition by the International Union of Pure and Applied Chemistry, the term biodegradation is “Breakdown of a substance catalyzed by enzymes in vitro or in vivo.
The term is often used in relation to ecology, waste management, biomedicine, and the natural environment (bioremediation) and is now commonly associated with environmentally friendly products that are capable of decomposing back into natural elements.
Biodegradable matter is generally organic material such as plant and animal matter and other substances originating from living organisms, or artificial materials that are similar enough to plant and animal matter to be put to use by microorganisms.
Bioremediation is the use of microorganisms (e.g., bacteria, fungi), plants (termed phytoremediation), or biological enzymes to achieve treatment of hazardous waste. Treatment can target a variety of media (wastewater, groundwater, soil/sludge, gas) with several possible objectives (e.g., mineralization of organic compounds, immobilzation of contaminants). In situ bioremediation (ISB) is the application of bioremediation in the subsurface – as compared to ex situ bioremediation, which applies to media readily accessible aboveground (e.g., in treatment cells/soil piles or bioreactors). In situ bioremediation may be applied in the unsaturated/vadoze zone (e.g., bioventing) or in saturated soils and groundwater (Sharma S. 2012).
Bioremediation is the process in which the micro-organisms are used to degrade the pollutants from the environment. Plants and micro-organisms are used to clean up the environment. Bioremediation is carried out by microbes and their metabolisms are used to remove the contaminants. Microbes have the ability to resolve the issue of contaminated ecosystem1. To improve or better living style the degradation of contaminated areas is very important. Importance of the biodegradation is increasing due to the expensiveness of the chemicals. So bioremediation is the best choice. The effluents should be degraded from the environment because they are very dangerous and have a bad impact on human beings. These pollutants sink into the water and cause pollution. These pollutants are treated with the help of microbes in bioremediation process. It is the best method because it is cost effective and eco-friendly. Different techniques of bioremediation are used to convert toxic substances into less toxic substances.
PHYTOREMEDIATION IN ENVT. MANAGEMENT - BIOTECHNOLGY ROLE...KANTHARAJAN GANESAN
It deals with, the various technologies involved in phytoremediation, mechanism, factors and biotechnology interventions for the improvement of remediation process etc...
The USEPA defines biodegradation as a process by which microbial organisms transform or alter (through metabolic or enzymatic action) the structure of chemicals introduced into the environment.
According to the definition by the International Union of Pure and Applied Chemistry, the term biodegradation is “Breakdown of a substance catalyzed by enzymes in vitro or in vivo.
The term is often used in relation to ecology, waste management, biomedicine, and the natural environment (bioremediation) and is now commonly associated with environmentally friendly products that are capable of decomposing back into natural elements.
Biodegradable matter is generally organic material such as plant and animal matter and other substances originating from living organisms, or artificial materials that are similar enough to plant and animal matter to be put to use by microorganisms.
LABORATORY STUDIES ON THE BIOREMEDIATION OF SOIL CONTAMINATED BY DIESEL IAEME Publication
The most widely used energy and fuel resources are hydrocarbons such as crude oil and petroleum distillates. The accidental discharge of these petroleum products contribute in making hydrocarbons the most common environmental pollutants. Bioremediation helps to destroy or render harmless various contaminants using natural biological activity. The present study utilizes the potential of bioremediation to remediate soil contaminated with diesel. Eight bioreactors were used for the study, out of which four bioreactors were maintained at optimum environmental conditions and the remaining four were kept without any maintenance to serve as control bioreactors. Contaminated soil was prepared by mixing fresh soil and diesel so as to attain 10% TPH concentrations by weight of soil. Each bioreactor was filled with 3 kg of contaminated soil.
Soil Pollution and It's effects on human health . Soil is a renewable source which maintain balance in ecosystem . Disturbance of this ecosystem balance by soil pollution can lead adverse effects not only human but also plants and other living organism . Many of the adverse Effects is seen by duration of exposure short term or long term . FDA monitors the effects on human health by soil pollution. Soil pollution is defined as the presence of toxic chemicals (pollutants or contaminants) in soil, in high enough concentrations to pose a risk to human health and/or the ecosystem .
Soil pollution impacts, treatment and controlMohamed Mohsen
This lecture gives the complete details of soil pollution impacts, remediation, and finally the possible ways for control.
The lecture was performed in Alexandria University by Dr.M.Mohsen and his colleague Rania Ahmed in August 2017
PHYTOREMEDIATION - Using Plants To Clean Up Our Environment - By HaseebHaseeb Gerraddict
Phytoremediation is the direct use of green plants and their associated microorganisms to stabilize or reduce contamination in soils, sludges, sediments, surface water, or ground water.
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
UNDERSTANDING WHAT GREEN WASHING IS!.pdfJulietMogola
Many companies today use green washing to lure the public into thinking they are conserving the environment but in real sense they are doing more harm. There have been such several cases from very big companies here in Kenya and also globally. This ranges from various sectors from manufacturing and goes to consumer products. Educating people on greenwashing will enable people to make better choices based on their analysis and not on what they see on marketing sites.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Alert-driven Community-based Forest monitoring: A case of the Peruvian Amazon
Heavy oils treatment
1. GROUP 5
1. YASMIN NABILAH BINTI MOHD FAUZEE
2. NURUL AFIQAH BINTI ANUAR
3. MOHAMMAD SUHAIL BIN SHOAIB
4. WAN ALI ABBAS BIN WAN MUSTAFA KAMAL
5. HASRIZAL BIN ABDUL HADI
4. Hydrocarbon
• Polyaromatic (PAH
compounds)
• Acyclic alkanes
• Aliphatic petroleum
hydrocarbon
Non-
hydrocarbon
• Heteroatoms
• Trace metals
(Mrozik, Piotrowska-Seget, & Labuzek,
2003)(Lee Jr & Von Lehmden, 1973)
•benz(a)anthracene and
•benzo(a)pyrene.
Carcinogenic to humans
International Agency for
Research on Cancer (IARC)
(Petry, Schmid, & Schlatter,
1996)
•Heavy paraffin etc
•Fire or explosion hazard
•Effects on the CNS or as general
asphyxiants or irritants
•Pneumonia
(Irwin, Van Mouwerik, Stevens,
Seese, & Basham, 1997)
•Methane, Ethane
•Propane, Butane
•Asphyxia
•Explosion
(Vale & Meredith, 1981)
•S, N, Ni, Fe and trace metals.
•Lung, nose, larynx and prostate
cancer
• Sickness and dizziness after
exposure to nickel gas
•Respiratory failure
• Birth defects
(Das, Das, & Dhundasi, 2008)
5. IMPACT OF
HEAVY OIL
LEAKAGE
reduce the ability of
soil to support the
growth of plants
seep into ground to
contaminate ground
water(Adams, Fufeyin, Okoro,
& Ehinomen, 2015)
Bioremediation uses microbial metabolism in the presence of optimum
environmental conditions and sufficient nutrients to breakdown contaminants
notably petroleum hydrocarbons. (Adams, Fufeyin, Okoro, & Ehinomen, 2015)
6. THERMAL
TREATMENT
• Rely on addition of
heat on soil to
increase the removal
efficiency of volatile
and semi-volatile
compound.
• E.g. Thermal
desorption,
smoldering,
incineration
PHYSICAL AND
CHEMICAL
TREATMENT
• Uses the physical
properties of the
contaminants or the
contaminated
medium to destroy
(i.e., chemically
convert), separate,
or contain the
contamination.
• E.g. Soil vapor
extraction, chemical
oxidation
BIOLOGICAL
TREATMENT
• Uses microorganisms
or vegetation to
degrade, remove, or
immobilize
contamination in soil.
1 2 3
7. PHYSICAL AND CHEMICAL
TREATMENT
•- Can be completed in
short time periods
- As the pollutants are
under vacuum there is
little chance of an
environmental release
during the application of
this technique
- Undergoes rapid
oxidation reactions;
- Ensures direct and
immediate contact with
chemical oxidation
PROS
-Exhaust air from in-situ
SVE system may require
treatment
- Soil with a high
percentage of fines and a
high degree of saturation
will require higher
vacuums (increasing
costs) and/or hindering
the operation of the in
situ SVE system
CONS
FINES = FINE PARTICLES , often of inorganic
material such as silica , frequently with metals and
organic compounds on their surfaces. Vary greatly
in size. Also known as PM (particular matter).
PM = PARTICULATE MATTER (PM), also known as
particle pollution, is a complex mixture of extremely
small particles and liquid droplets that get into the
air. Once inhaled, these particles can affect the
heart and lungs and cause serious health effects.
(EPA, 2017)
1
ed
8. • The gas leaving the soil may be treated to
recover or destroy the contaminants,
depending on local and state air discharge
regulations.
• During incineration process, organic pollutants
may release as vapour, or bound to
particulates. Dioxin is the organic pollutants
that attracts most concern.
• The exhaust gases are filtered in scrubber,
electrostatic precipitators, or baghouses and
subsequently incinerated to remove any
gaseous products that cannot be vented due
to air pollution and soil deposition concerns
ed
9. - Eliminate large
volumes of oil quickly
and effectively
-broad applicability and
prevalence
- Energy intensive
- Costly
- Can damage soil
properties
THERMAL TREATMENT
Vidonish, J. E., et al. (2016)2
10. BIOREMEDIATION
Das, N. and P. Chandran (2011).
- environmental-friendly
- For a long time benefit
- to be noninvasive and relatively cost-effective
-several mechanism for removal
- Reduces landfill waste harvestable plant material
- Micro-organisms which are existent in the soil use
oil compounds as the sources of carbon and energy
-may take time
-depend on soil properties, water availability,
and the heat sensitivity of contaminated soils
- Climate dependent
PROS
CONS
3
11. TREATMENT PROPOSAL OF IN SITU
BIOREMEDIATION
Step 1 : Biosurfactant
Step 2 : Bioaugmentation
Step 3 : Biostimulation
Step 4 : Biocatalysis
Step 5 : Phytoremediation
ADVANTAGES OF IN-SITU
BIOREMEDIATION
• Typically less expensive
• No need to excavate & transport
soils
• Can treat a large volume of soil
at once
• Causes less contaminants to be
released than ex situ techniques
• Creates less dust
(Perelo, 2010)
12. 1. Contaminated area washed with out by using a water solution of biosurfactant, and
partially removed the oil.
a. The collected oil can be used again
b. The biosurfactant easily biodegraded into a common natural compound
14. A) Use of Microbes To Degrade Hydrocarbon Contamination.
● Biodegradation of oil spill can be achieved through microbes utilization.
● One of the main approache to oil spill bioremediation:
○ Bioaugmentation – introducing new microbes to the existing microbial
population
○ Bacteria
○ Fungi
● The success of bioaugmentation success depends on the competitiveness of the
inoculated strains in different environments.
● Therefore it is important to establish and maintain conditions that favor enhanced
oil biodegradation rates in the contaminated environmental depends.
● Two major challenges:
○ Lack of important nutrients
○ Low bioavailability
15. B) Used Of Microbes To Lower Contamination Concentration
• - Using Bacteria Consortium.
• Use of bacteria consortium consisting of
Pseudomonas aeruginosa and Rhodococcus
erythropolis.
• The consortium degraded 91% of the hydrocarbon
content of soil contaminated with 1% (v/v) crude oil
sludge in 5 weeks (Cameotra and Singh, 2008).
• The bacteria also able to degrade liquid paraffin
more than 70% in 180 days (Zhukov et al., 2007).
• More than 98% hydrocarbon depletion was obtained
when both additives were added together with the
consortium.
• Transport of hydrocarbon across the cell membrane can
follow three main mechanisms: (1) passive diffusion; (2)
passive facilitated diffusion; or (3) energy-
dependent/active uptake (Huan and Wang, 2014).
Figure 1: Main principle of aerobic degradation
of hydrocarbons by microorganisms (Das and
Chandran, 2011)
ed
16. B) Used of Microbes To Lower Contamination Concentration
• - Using White Rot Fungi.
● Compared to bacteria, some white rot species are
better able to colonize soil and to compete with the
other microflora (Novotný et al., 2000).
● Intracellular degradation of highly condensed PAH is
limited due to their low solubility and their restricted
transport through the cell membrane.
● the ability of fungal hyphae to reach the pollutants by
penetrating contaminated soil, combined with the
production of extracellular oxidases, gives fungi a
significant advantage over bacteria (Pointing, 2001).
Mechanism for PAH degradation using
lignolytic degradation (Pointing, 2001)
18. • Addition of carbon, nitrogen, phosphorus and potassium (CNPK). Only add the
undetectable needed nutrient on site. (F. Benyahi and A.S. Embaby, 2015)
• The addition of nutrients into treatment site by form of biosolid or inorganic
fertilizer.
• Biosolid is made up of combination of manure and plant waste such as peanut hull
and rice straw. (W. Liu, Y. Luo, Y. Teng, Z. Li, and Q. M, Lena, 2010)
• Also known as bulking agent
• Inorganic fertilizer are consist of NPK
• Addition of biosurfactant
The addition of limiting nutrients to support
microbial growth
20. • Contributing to minimising fossil fuel damages and reduce
generate toxic by-products.
• Need to have the capacity to degrade the target contaminant
into less-toxic product which is do not depend on cofactor.
• Types of enzymes : Laccases, Alkane hydroxylases.
(R. S. Peixoto, A. B. Vermelho, and A. S. Rosado, (2011))
Utilization of enzymes to enhanced the
degradation
21. Phytoremediation to degrade to an acceptable level or render harmless the contaminants
in the soil.
Can be achive through rhizodegradation (phytostimulation)
• Degradation of contaminant in the rhizospehre by means of microbial activity which is
enhanced by the presence of plant roots in severals way (Anderson 1993):
1. The roots released compounds, such as sugars, organic acids, and alcohol which will enrich
indigenous microbe populations
2. root systems bring oxygen to the rhizosphere, which ensures aerobic transformations
3. mycorrhizae fungi, which grow within the rhizosphere, can degrade organic contaminants that
cannot be transformed solely by bacteria because of unique enzymatic pathways.
• A comparison performed in New Jersey using fine-rooted grasses showed that phytostimulation
ranges from $10 to $35 per ton of soil. Other technologies, such as incineration, range from $200 to
$1,000 per ton of soil
22. Plant roots uptake metal
contaminants from the soil
Translocate them from the
root to aerial tissues (stems
and leaves)
Transform into carbonate, sulphate, or
phosphate precipitate and immobilizing
them in apoplastic (extracellular) and
symplastic (intracellular) compartments
Process of Phytoextraction (Lasat, 2000; Raskin et al., 2000).
• Phytoremediation used plants to break down or degrade organic pollutants or remove and stabilize metal
contaminants.
• A plant used for phytoremediation needs to be heavy-metal tolerant, grow rapidly with a high biomass yield
per hectare, have high metal-accumulating ability in the foliar parts, have a profuse root system, and a high
bioaccumulation factor (Scragg, 2006).
• Hyperaccumulator plant species are used on metalliferous sites due to their tolerance of relatively high level
of pollution.
Phytoremediation
23. Figure 7: Strategies for phytoremediation. Figure 8: Long-term phytoextraction sunflower
plantation field site in Bettwiesen, Switzerland.
Examples: Willow (Salix viminalis L.), Indian
mustard (Brassica juncea L.), corn (Zea mays L.),
and sunflower (Helianthus annuus L.) have
reportedly shown high uptake and tolerance to
heavy metals (Schmidt, 2003).
24. Degradation by enzyme produced by plant or via metabolic processes within the plant
Phytotransformation
• 2 processes can occurs which are (1) storage of chemicals into plant via LIGNIFICATION, and (2) complete
conversion to carbon dioxide and water (mineralization).
• Certain enzymes produced by plants are able to breakdown and convert chlorinated solvent (e.g.,
trichloroethylene)
The release of volatile contaminant to the atmosphere via plant transpiration.
Phytovolatilization
• The metal and metalloids are converted into less toxic and volatile form.
• Modified by plant and released to atmosphere
Other mechanisms of phytoremediation used in contaminated
soil remediation
25. CONCLUSION
• In order to solve the environmental issue involving heavy oils wholly, the suggested plans to
perform in-situ treatment is starting from:
• Bio-surfactant will aid in removing the oil that contaminate the soil. Besides that, this also could
increase the bioavailability of the contaminants.
• However, the main steps will be the next 4 steps. As there are involvements of biological
organisms as well as catalyst to help in removal of contaminants completely.
ed
Step 1 :
Biosurfactant
Step 2 :
Bioaugmentation
Step 3 :
Biostimulation
Step 4 : Biocatalysis
Step 5:
Phytoremediation
26. • Introduction of microbes (fungi) into the system
• To degrade hydrocarbon contamination & to lower contamination concentration
• White rot fungi will degrade the hydrocarbon better compared to bacteria
• The fungi able to degrade the hydrocarbon using extracellular enzymes and it can
penetrate deeper in soil using the hyphae.
Step 1 : Bioaugmentation
• Use biosolid as a method of delivery. It consist of nitrogen source in the form of urea
and plant waste such as rice straw.
• It can increase the nutrient content.
• Plant waste make the soil become more porous which enhance the dispersion microbe
into the whole part of soil by supplying the water flow and give good aeration.
Step 2 : Biostimulation
• Enzymatic remediation can be simpler than working with whole organism.
• Can be increased in laboratory conditions.
• Use laccases which can easily get from Myceliophtora thermophila and Saccharomyces
cerevisae. Capable catalyzing the oxidation of phenols, polyphenols and anilines.
Step 3 : Biocatalysis
• For Hydrocarbon contaminants : Using phytostimulation (enhance microbes for
hydrocarbon degradation.)
• For heavy metals contaminants : Ni, Cd, Pb, Cu (sunflower, indian mustard)
• Reason : They are good hyper accumulator and have high tolerance to heavy metals.
• They will be extracted from the sites and are not for consumption and to feed the
others.
Step 4 : Phytoremediation
ed
Biotechnology application proposed to treat heavy oils:
27. REFERENCE
1. Das, N., & Chandran, P. (2011). Microbial degradation of petroleum hydrocarbon contaminants: an overview. Biotechnology research international, 2011.
2. Cameotra, S. S., & Singh, P. (2008). Bioremediation of oil sludge using crude biosurfactants. International Biodeterioration & Biodegradation, 62(3), 274-280.
3. Eggen, T., & Majcherczyk, A. (1998). Removal of polycyclic aromatic hydrocarbons (PAH) in contaminated soil by white rot fungus Pleurotus ostreatus. International Biodeterioration & Biodegradation, 41(2), 111-117.
4. Novotný, Č., Erbanova, P., Cajthaml, T., Rothschild, N., Dosoretz, C., & Šašek, V. (2000). Irpex lacteus, a white rot fungus applicable to water and soil bioremediation. Applied Microbiology and Biotechnology, 54(6),
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USA.
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6, no. 2, pp. 249– 258.
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3, pp. 485–496.
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1–25.
24. I. Raskin and B. D. Ensley. (2000). Phytoremediation of Toxic Metals: Using Plants to Clean Up the Environment, JohnWiley & Sons, New York, NY, USA.
25. U. Schmidt.. (2003). “Enhancing phytoextraction: the effect of chemical soil manipulation on mobility, plant accumulation and leaching of heavy metals,” Journal of Environmental Quality, vol. 32, no. 6, pp.
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