This presentation based on heat shock proteins.... Which is more important in regulation of different stages of life cycle of organisms. This presentation based on my project work. Isolation of heat shock Proteins from xerophyte....
The Hedgehog pathway was discovered in fruit fly (Drosophila) and is conserved in vertebrates (including humans)
The Hedgehog pathway is involved in cell growth and differentiation to control organ formation during embryonic development.
Hedgehog signalling regulates embryonic development, ensuring that tissues reach their correct size and location, maintaining tissue polarity and cellular content.
In the skin, the Hedgehog pathway is critical for regulating hair follicle and sebaceous gland development.
Germline mutations in components of the Hedgehog signalling pathway results in a number of developmental abnormalities.
Hedgehog signalling normally remains inactive in most adult tissues
microbial energetics. heat shock responses by the gram negative and gram positive bacteria by the protein synthesis mechanism, by those bacteria which are mesophiles in the nature and can survive onlyb at room tempertature.
This presentation based on heat shock proteins.... Which is more important in regulation of different stages of life cycle of organisms. This presentation based on my project work. Isolation of heat shock Proteins from xerophyte....
The Hedgehog pathway was discovered in fruit fly (Drosophila) and is conserved in vertebrates (including humans)
The Hedgehog pathway is involved in cell growth and differentiation to control organ formation during embryonic development.
Hedgehog signalling regulates embryonic development, ensuring that tissues reach their correct size and location, maintaining tissue polarity and cellular content.
In the skin, the Hedgehog pathway is critical for regulating hair follicle and sebaceous gland development.
Germline mutations in components of the Hedgehog signalling pathway results in a number of developmental abnormalities.
Hedgehog signalling normally remains inactive in most adult tissues
microbial energetics. heat shock responses by the gram negative and gram positive bacteria by the protein synthesis mechanism, by those bacteria which are mesophiles in the nature and can survive onlyb at room tempertature.
Cellular Signaling Pathways have direct implications on our understanding of tumor cell behavior. A general overview is presented here followed by a brief discussion of some of the major pathways currently implicated in cancer progression : Ras/RAF/MAP kinase pathway and PI3K/AKT/mTOR pathway s
Signal transduction Calcium Signaling vibhakhanna1
A wide range of Ca2+ signaling pathways deliver the spatial and temporal Ca2+ signals necessary to control the specific functions of different cell types, via various effector proteins and protein kinases
The process of transcription is the first stage of gene expression resulting in the production of a primary RNA transcript from the DNA of a particular gene.
This step of gene expression which is followed by a number of post-transcriptional processes such as RNA splicing and translation.
These lead ultimately to the production of a functional protein and this process is highly regulated.
Both basal transcription and its regulation are dependent upon specific protein factors known as transcription factors.
These highly specific protein bind to the specific regulatory gene of DNA sequence and control the transcription process and regulate it.
For example- enzyme RNA polymerase catalyzes the chemical reaction that synthesize RNA, using the DNA gene as a template, the transcription factor control when, where, and how efficiency RNA polymerase function.
Play an important role in the normal development and routine of cellular function.
An oncogene is a gene that has the potential to cause cancer. In tumor cells, they are mutated or expressed at high levels. Most normal cells undergo a programmed form of rapid cell death (apoptosis) when critical functions are altered.
Therapeutic Club on Tauopathy. Its pathophysiology and therapeutic targets. Interactive session held at All India Institute of Medical Sciences, New Delhi - 110029 on 28th October 2017.
Cellular Signaling Pathways have direct implications on our understanding of tumor cell behavior. A general overview is presented here followed by a brief discussion of some of the major pathways currently implicated in cancer progression : Ras/RAF/MAP kinase pathway and PI3K/AKT/mTOR pathway s
Signal transduction Calcium Signaling vibhakhanna1
A wide range of Ca2+ signaling pathways deliver the spatial and temporal Ca2+ signals necessary to control the specific functions of different cell types, via various effector proteins and protein kinases
The process of transcription is the first stage of gene expression resulting in the production of a primary RNA transcript from the DNA of a particular gene.
This step of gene expression which is followed by a number of post-transcriptional processes such as RNA splicing and translation.
These lead ultimately to the production of a functional protein and this process is highly regulated.
Both basal transcription and its regulation are dependent upon specific protein factors known as transcription factors.
These highly specific protein bind to the specific regulatory gene of DNA sequence and control the transcription process and regulate it.
For example- enzyme RNA polymerase catalyzes the chemical reaction that synthesize RNA, using the DNA gene as a template, the transcription factor control when, where, and how efficiency RNA polymerase function.
Play an important role in the normal development and routine of cellular function.
An oncogene is a gene that has the potential to cause cancer. In tumor cells, they are mutated or expressed at high levels. Most normal cells undergo a programmed form of rapid cell death (apoptosis) when critical functions are altered.
Therapeutic Club on Tauopathy. Its pathophysiology and therapeutic targets. Interactive session held at All India Institute of Medical Sciences, New Delhi - 110029 on 28th October 2017.
Heat stress as well as other stresses can trigger some mechanisms of defense such as the obvious gene expression that was not expressed under “normal” conditions.
The sudden changes in genotypic expression resulting in an increase in the synthesis of protein groups. These groups are called “heat-shock proteins” (Hsps), “Stress-induced proteins” or “Stress proteins”
Blocking anti-inflammatory antibodies to histamine/serotonin receptors is a prospective method of medical management of Acute Radiation Disease and can inhibit pro inflammatory cascades and possible damage of internal organs of irradiated mammals.
Basic Mutagenic signal Transduction or the cancer signal transduction that control cell cycle are important pathways to understand cancer in molecular level and to invent targeted treatment.
The brain orchestrates organ function and regulates whole body metabolism by the concerted action of neurons and glia cells in the central nervous system. To do so, the brain has tremendously high energy consumption and relies mainly on glucose utilization and mitochondrial function in order to exert its function. As a consequence of high-rate metabolism, mitochondria in the brain accumulate errors over time, such as mitochondrial DNA (mtDNA) mutations, reactive oxygen species, and misfolded and aggregated proteins. Thus, mitochondria need to employ specific mechanisms to avoid or ameliorate the rise of damaged proteins that contribute to aberrant mitochondrial function and oxidative stress. To maintain mitochondria homeostasis (mitostasis), cells evolved molecular chaperones that shuttle, refold, or in coordination with proteolytic systems, help to maintain a low steady-state level of misfolded/aggregated proteins. Their importance is exemplified by the occurrence of various brain diseases which exhibit reduced action of chaperones. Chaperone loss (expression and/or function) has been observed during aging, metabolic diseases such as type 2 diabetes and in neuro de-generative diseases such as Alzheimer’s (AD), Parkinson’s (PD) or even Huntington’s (HD) diseases, where the accumulation of damage proteins is evidenced. Within this perspective, proper brain function is maintained by the joint action of mitochondrial chaperones to ensure and maintain mitostasis contributing to brain health, and that upon failure, alter brain function which can cause metabolic diseases.
The main focus of discovery biology for the past few years has been in the areas of diabetes, cardiovascular, cancer, infl ammation and anti-infective. One of the key out of box approach for combating diabetes has been the regulation of key protein AMP kinase. For cancer therapy, several of the signal transduction kinases like ERK kinase, MAP kinase, Aurora kinase etc have been used as key protein targets. Kinases are enzymes that regulate downstream pathways by post translational modifi cation (phosphorylation) of target proteins. Thus the detection of phosphorylation state of a protein is very important in these studies. Classically kinase assays were and are performed using radiolabeled P32 or P33 antibodies that have serious disadvantages. The regulation of proteins at the gene and mRNA level can be very well studied by genomics technology but the post translational modifi cation of proteins can be studied exclusively by proteomic strategies. In view of the tremendous importance of kinases to discovery research, a non-radioactive, non-antibody, high throughput and safe assay for detection of in vivo phosphorylation of proteins -the proteomic way is proposed.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
6. Heat Shock Proteins (HSPs)
Categorized, according to their molecular weights, into
various families
• HSP 90
• HSP 70
• HSP 60
• HSP 40
• Small HSPs or α crystalline proteins
7. Major functions
Family Function
HSP90 Regulatory interactions with signalling proteins, stabilization of
misfolded proteins
HSP70 Protein folding, membrane transport of proteins, auto regulation if
heat shock response, anti-apoptotic
HSP60 Protein folding (limited to cytoplasm)
HSP40 Protein folding, co-chaperon for HSP70
Small
HSPs
Stabilization of misfolded proteins, eye lens structural proteins
8.
9. HSP 90
In cancer cells, enhances growth, supresses senescence,
provides resistance to stress induced apoptosis (i.e. confers
protection against apoptosis due to cytotoxic drugs and
radiation therapy)
11. Efungumab
• Fungi, in stress, produce HSP90 which confers resistance.
• Efungumab, a HSP90 inhibitor, was one such anti-fungal to
be introduced for the treatment of invasive candidiasis.
12. HSP 70
• In cancer cells, enhances growth, supresses senescence,
provides resistance to stress induced apoptosis (i.e. confers
protection against apoptosis due to cytotoxic drugs and
radiation therapy)
• Triptolide
• Used in pancreatic cancer
• Induces apoptosis by inhibiting HSP70
• BIIB021, a HSP90 inhibitor, enhances activity of Triptolide
13. HSP 70
• In sympathetic neurons,
• HSP 72 – inhibits degradation of Tau protein, heat shock inducible.
• HSC 70 - promotes degradation of Tau protein.
• Methylene blue – inhibits ATPase activity of HSP70 (72)
• Reduces Tau polyglutamine formation
• Improves cognition in Alzheimer’s disease
• Phase IIb
14. HSP 60
• In the mitochondira, replication and transcription of DNA,
pro – survival.
• In the cytosol, it forms complexes and inhibits maturation
and activation of Caspase 3 – Anti apoptotic
• Epolacteane tertiary butyl ester (ETB) – prevents binding of
HSP60 with mtf1, thereby preventing mitochondrial
transcription - Apoptosis
15. HSP 60
• Surface and extracellular
• Stimulates immune response
• Bortezomib – increases expression of HSP 60 on
malignant cells and thus enhances immune response
against tumour cells.
16. HSP 40
• Excess HSP40-HSP70 complex leads to accumulation of
polyglutamine proteins Neurodegenerative diseases.
• Quercetin (Pre-clinical, Inhibits HSP 40 and 27)
17. HSP 27 (HSP β1)
• Anti-oxidant (increases glutathione levels and decreases
iron levels)
• Anti-apoptotic in response to chemicals (inhibits liberation
of cytochrome c from mitochondria preventing
proteolysis)
• Apatorsen (Phase II)
18. HSP 27
As a diagnostic tool
• Increased in – Renal injury and fibrosis, Cancers of
breast, lung, liver, prostate, rectal, osteosarcoma,
leukemia, cerebral and cardiac ischemia
• Reduced in – oesophageal cancer
• Anti-HSP27 IgA – Gyneacological malignancies
19. Conclusion
• In todays date and time, emphasis is laid on cancer
chemotherapy and ‘n’ number of drugs are under clinical
trails for the same.
• Sure, these will be a boon to the health care system in the
near future, however diverting focus on neurodegenerative
disorders would be a welcome change.
• Similarly, bacteria and fungi also produce HSPs when under
stress, exploiting this area will help cope with the stress of
‘antibiotic resistance’.
Editor's Notes
Cancers and neurodegenerative diseases
Parkinsons - Lewy bodies
Stress – change in temp, pH, oxidizing agents, heavy metals, poisonous gases.
HSP – Stabilize proteins, provide stress free environment
60 - (Chaperonin)
HSP100 Stress tolerance, thermo-tolerance, protein disaggregation
Cancer and neurodegenerative diseases
Growth – Erb2, Met
Senescence – binds to telomerase
With Imatinib (CML – reduction in Bcr-abl) bortezomib, arsenic trioxide, doxorubicin
All under phase 1 for various cancers
Radicilol – Inhibits hsp90-client protein complex signalling.