This document discusses the importance of proper hand washing and use of personal protective equipment (PPE) in healthcare settings. It provides objectives and a PICO question regarding the use of these practices reducing nosocomial infections. Background, history, guidelines and techniques for effective hand washing and proper PPE use are outlined. Statistics on healthcare-associated infections and the impact of these practices on costs, patient and healthcare worker health are also presented. Finally, challenges, research and conclusions supporting the use of hand washing and PPE to prevent infection spread are summarized.