Gingivectomy is a procedure to remove a portion of gum tissue to treat gum disease or correct gum conditions around the teeth. It involves making incisions along the gumline and removing diseased gum tissue using scalpels or lasers. This helps reduce gum pockets and allows proper cleaning under the gumline. Gingivectomy can be done surgically using scalpels or via other methods like chemotherapy, electrosurgery, cryosurgery or lasers. Maintaining good oral hygiene is important after gingivectomy for proper healing and prevention of future gum problems.
Gingivectomy and gingivoplasty are the periodontal surgical procedures. It was first introduced by Pierre fauchard. It is used in pocket elimination by gingival resection whereas gingivoplasty refers to recontouring of gingiva in the absence of pockets.
Gingivectomy means excision of the gingiva.
Gingivoplasty is a reshaping of the gingiva to create physiologic gingival contours with the sole purpose of recontouring the gingiva in the absence of pockets.
ROS is a substractive method of having positive bone architecture. it includes osteotomy and ostectomy procedures. osteotomy is to remove non supporting bone and ostectomy is to remove supporting bone for having positive bony architecture. there is definitive osseous surgery and compromise osseous surgery. transgingival probing is a method of determining osseous topography. various hand and rotary instruments are use for this procedure.
Gingivectomy and gingivoplasty are the periodontal surgical procedures. It was first introduced by Pierre fauchard. It is used in pocket elimination by gingival resection whereas gingivoplasty refers to recontouring of gingiva in the absence of pockets.
Gingivectomy means excision of the gingiva.
Gingivoplasty is a reshaping of the gingiva to create physiologic gingival contours with the sole purpose of recontouring the gingiva in the absence of pockets.
ROS is a substractive method of having positive bone architecture. it includes osteotomy and ostectomy procedures. osteotomy is to remove non supporting bone and ostectomy is to remove supporting bone for having positive bony architecture. there is definitive osseous surgery and compromise osseous surgery. transgingival probing is a method of determining osseous topography. various hand and rotary instruments are use for this procedure.
mucogingival surgery or plastic surgery of muco-gingival tissue is a surgical procedure targeted to correct and eliminate anatomic, developmental and traumatic alterations of gingiva.
Periodontitis is a complex infection initiated by bacteria –tissue destruction.
Host: the organism from which a parasite obtains its nourishment/ an individual who receives a graft
Modulation: the alteration of function or status of something in response to a stimulus or an altered physical or chemical environment
REFERENCES TAKEN FROM CARRANZA'S TEXTBOOK OF CLINICAL PERIODONTOLOGY AND LINDHE'S TEXTBOOK OF CLINICAL PERIODONTOLOGY AND IMPLANT DENTISTRY. CONTAINS ENOUGH AND MORE DETAILS OF THIS TOPIC FOR BDS STUDENTS.HOPE THIS PRESENTATION WILL HELP U GAIN SOME KNOWLEDGE ABOUT PERIODONTAL PLASTIC AND ESTHETIC DENTISTRY.
mucogingival surgery or plastic surgery of muco-gingival tissue is a surgical procedure targeted to correct and eliminate anatomic, developmental and traumatic alterations of gingiva.
Periodontitis is a complex infection initiated by bacteria –tissue destruction.
Host: the organism from which a parasite obtains its nourishment/ an individual who receives a graft
Modulation: the alteration of function or status of something in response to a stimulus or an altered physical or chemical environment
REFERENCES TAKEN FROM CARRANZA'S TEXTBOOK OF CLINICAL PERIODONTOLOGY AND LINDHE'S TEXTBOOK OF CLINICAL PERIODONTOLOGY AND IMPLANT DENTISTRY. CONTAINS ENOUGH AND MORE DETAILS OF THIS TOPIC FOR BDS STUDENTS.HOPE THIS PRESENTATION WILL HELP U GAIN SOME KNOWLEDGE ABOUT PERIODONTAL PLASTIC AND ESTHETIC DENTISTRY.
Periodontal plastic surgery is defined as the surgical procedures performed to correct deformities of the gingiva or alveolar mucosa. It includes widening of attached gingiva,
deepening of shallow vestibules, resection of the aberrant frena, depigmentation of gingiva.In all of these procedures, blood supply is the most significant concern and must be the underlying issue for all decisions regarding the individual surgical procedure.
ART - Atraumatic Restorative Treatment.pptxDrLasya
DEFINITION
ART is defined as a minimally invasive care approach in preventing dental caries and stopping its further progression.
- Jo E. Frencken, 2012
PRINCIPLES
Removing carious tooth tissue using hand instruments only
INDICATIONS
Only in small cavities (involving dentin)
In those cavities that are accessible to hand instruments.
Public health programs
CONTRA INDICATIONS
Presence of swelling (abscess) or fistula (opening from abscess region to the oral cavity) near the carious tooth.
Pulp exposure
Chronic inflammation of the pulp with pain in the tooth
There is an obvious carious cavity, but the opening is inaccessible to hand instruments
There are clear signs of a cavity for example in a proximal surface, but the cavity cannot be entered from the proximal or the occlusal direction.
Reasons for using hand Instruments:
With this technique, restorative care is made available to all population groups.
This technique is said to be tooth friendly as this conserves sound tooth tissues and causes less trauma to the teeth by requiring minimal cavity preparation.
Cost effective technique as this uses hand instruments in place of costly electrically driven dental equipment.
Use of local anesthesia for pain management is minimal there by reducing the psychological trauma to patients.
Hand instruments are easy clean and sterilize after every use, thus making infection control simplified
Reasons for using GIC
GIC sticks chemically both to enamel and dentine- the need to cut sound tooth tissue to prepare the cavity is reduced.
Fluoride is released from the restoration which will prevent and arrest caries.
Biocompatible cement as this restoration does not cause any irritation to pulp and gingiva, and has a co-efficient of thermal expansion similar to tooth structure.
Operators work posture:
Operator eye to patient tooth- 30 to 35 cms
Positions – Direct rear - 12 o’ clock
Right rear – 10 o’clock position
Assistant head - 10 to 15 cms higher than operator
Patient Position
Lying on back on flat surface
Head rest- firm foam or rubber ring
ADVANTAGES
Biological approach
Painless
Simplified infection control
No electrically driven and expensive dental equipment is needed
Technique is simple enough to train non-dental personnel or primary health care workers
Cost effective
Friendly procedure
Procedure:
1. Carious dentin can be removed with Spoon excavator
2. Fracture off unsupported thin enamel with Dental hatchet
3. Again clean the cavity with wet cotton rolls. Dry the tooth surface with dry cotton rolls
4. Apply dentin conditioner in the prepared tooth cavity
5. Mix the required amount of GIC
6. Restore the prepared cavity with mixed GIC with the help of blunt end of applier
7. Press the restorative material with gloved finger
8. Remove excess restorative material with sharp end of applier
9. Check the occlusion using articulating paper
CONCLUSION
ART is a combined preventive and curative oral care approach
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
2. INTRODUCTION
• 80% of Filipinos suffer from dental problems
• Periodontal Disease is prevalent among elders
• GINGIVECTOMY may be performed to heal the effects of periodontal
disease or to correct a gum condition involving the structures around
the teeth
4. • Total removal of a portion
of a gingiva (gum) from in
and around the tooth in
order to treat gum disease
or to lengthen the height
or width of a teeth or
section of teeth
• one of a few procedures
that can help reverse
periodontal issues.
7. CONTRAINDICATIONS
• Bone defect cannot be corrected
• Fragile gingiva
• Location of the base of the pocket apical to
mucogingival junction
8. TYPES OF GINGIVECTOMY
1. SURGICAL GINGIVECTOMY
2. GINGIVECTOMY BY CHEMOSURGERY
3. GINGIVECTOMY BY ELECTROSURGERY
4. GINGIVECTOMY BY CRYOSURGERY
5. GINGIVECTOMY BY LASER
11. STEPS IN SURGICAL GINGIVECTOMY
1. Anaesthetize the area
2. Mark the pocket
3. Resect the gingiva
4. Remove the granulation tissue
5. Remove calculus
6. Place periodontal pack
12. WHAT IS POCKET MARKING?
• Pocket on each surface are explored
with periodontal probe and marked
with the pocket marker at three
places on each tooth on each labial
and lingual surfaces
• Pocket should be marked
systematically beginning on distal
surface of the last tooth the moving
on the facial surface and proceeding
anteriorly to the midline
13. TYPES OF INCISION
INTERNAL BEVEL INCISION
A. Discontinuous – from the facial surface at distal angle of last tooth to
distofacial angle of the next tooth. Next incision begins in the
interdental space to distofacial angle of next tooth
B. Continuous – started on the facial surface from the disto angular
region and carried forward anteriorly following the course of the
pocket without interruption. Procedure is repeated on the lingual
surface
C. Distal Incision – facial and lingual incision are joined by an incision
across the distal surface of the last erupted tooth
14.
15. STEPS IN SURGICAL GINGIVECTOMY
1. Start apical to points marking of the course of periodontal pocket and
is directed coronally to a point between the base of the pocket and
crest of the bone
2. Should be closed to bone but DO NOT exposed it
3. The incision should be beveled at approx. 45 degree to the tooth
surface to follow the normal festooned pattern of the gingiva
4. Should not leave diseased Pocket wall
5. The incision should pass completely through soft tissue to the tooth
16. • REMOVE RESECTED- GINGIVA
- Remove the marginal and interdental gingiva starting from distal surface of last tooth detached
gingiva at the line of incision with the help of surgical hoes and scalers
• APPRAISE THE FIELD
- Bead like granulation tissue
- Calculus remnants
- A band of light zone on the root surface
- Softening of root surface resorptions and cementum protuberances
• REMOVE GRANULATION TISSUE
- The curettes are used for this purpose. The curette is guided along the tooth surface and under
the granulation tissue
• REMOVE CALCULUS
- The remaining calculus and necrotic cementum are to be removed using scalers and curettes.
Check each surface of every tooth for calculus an soft tissue remnants.
- wash area several times with saline and cover with gauze sponge
17. • PLACE PERIODONTAL PACK
- After the bleeding is control and hemostasis achieved, the gingivectomy wound is covered with
periodontal pack
• HEALING AFTER SURGICAL GINGIVECTOMY
18. Initial response after gingivectomy is CLOT FORMATION
Underlying tissue become acutely inflamed with some necrosis
The clot is replaced by granulation tissue
After 12-24 hours epithelial cells at the margin start to migrate over the
granulation tissue separating it from the clot
Epithelial cells advanced by TUMBLING action
Surface epithelization is generally completed after 5 to 14 days
19. GINGIVECTOMY BY CHEMOSURGERY
Agent used
1. 25% phenol with 75% camphor
2. 5% paraformaldehyde in ZnO eugenol pack
Advantages:
1. No analgesia or anesthesia required for the procedure
2. Procedure is easy to perform & require less instruments
20. • Disadvantages
1. Bone necrosis might result
2. Periodontal abscess might result
3. Delayed wound healing
4. Subsequent plaque retention
5. Bone resorption
21. GINGIVECTOMY BY ELECTRO SURGERY
Advantages:
1. Less bleeding
Disadvantages:
1. Procedure produces heat which causes necrosis of adjacent tissue
2. If it transfer to the bone, resorption take place
22.
23. GINGIVECTOMY BY CRYOSURGERY
- Temperature -50 to -60˚c id applied
to gingiva by means of a probe
Advantages:
1. The procedure does not cause pain
and bleeding
24. GINGIVECTOMY BY LASER
Types of laser used:
1. CO2 laser
2. ND: YAG Laser
Advantages:
1. Similar to electrosurgery more sophisticated, produces no heat thereby,
least necrosis
2. Similar to electrosurgery no post operative dressing is required
25.
26.
27. MAINTENANCE AFTER GINGIVECTOMY
1. Prescribe Chlorhexidine Gluconate rinse
2. Advice patient to maintain good oral hygiene
3. Recall for professional cleaning
Editor's Notes
Incomplete gingival remodeling
Delayed epithelialization, CT repair
Increased inflammation after chemical trauma
No control over the depth of action