Plant Agriculture Biotechnology: Empower Your Plant Science Research

Plant Agriculture Biotechnology:
Empower Your Plant Science
Research
Full-spectrum genetic analysis tools and beyond

Outline
• The challenge: feeding and fueling the world
• Life Technologies mission and our portfolio plant agricultural biotechnology products
• Key applications for plant researchers and supporting data

Plant genome and transcriptome sequencing, and SNP discovery

Plant resequencing and SNP genotyping

QTL mapping and marker-assisted selection

GMO detection and screening

Plant genetic engineering

Plant gene expression

DNA and RNA isolation

• Summary

2 6/28/2012 | Life Technologies™ Corporation

http://documents.wfp.org/stellent/groups/public/documents/communications/wfp229328.pdf

Global challenges—population, food, and land
• World population expected to reach 8 billion by 2025*
− 20% of the world’s population is not receiving the minimum food required for a
healthy life
− Global demand for food will increase three times in the next 15 years
− Pests destroy 1/3 of the food produced globally ($30 billion spent on pesticides)
− 1.2 billion people globally exist in poverty, with earnings < $1/day

• Recent notable climate events
− Increase in intensity of natural disasters and extreme weather events
− Rising sea levels, contamination of water and agricultural land
− Changes in rainfall patterns and water shortage
> Hotter climates contributed to lower wheat (–5.5%) and corn (–3.8%) yields**

*Agricultural Biotechnology: A Global Strategic Business Report. Global Industry
Analysts, Inc. 10/2010.
**Agriculture: A Global Industry Outlook. Global Industry Analysts, Inc. 01/2012


Plant biotechnology offers many solutions

• Higher crop yield per acre
• Resistance to insect pests, diseases, droughts, and salt
• Lower production costs and lower environmental impact
• Foods with improved nutrient profiles
• Renewable energy sources
• New applications: high-value chemicals, plastics, vaccines, phytoremediation,
etc.


Life Technologies mission: to empower your plant science research
• Providing innovative platforms optimized for each step of
plant biotechnology workflows, from tools that help
elucidate the genetic makeup of plants to DNA
manipulation, gene and protein expression, cell imaging, and
copy number variation

• Offering the widest technology selection, with the
highest quality at every budget to help address
challenges like food production, land conservation,
and biodiversity protection


Key applications in plant biotechnology
QTL mapping
& marker-
assisted
selection

Plant de novo
GMO testing
genome
& detection
Desired phenotypes sequencing

Higher yields
Pest and environmental
stress tolerance
Healthier diets
More efficient fertilizer use
Biofuels
Nutraceuticals
Plant genetic Plant
engineering genotyping

Plant gene
expression
analysis


Plant Genome & Transcriptome
Sequencing

Plant genome and transcriptome sequencing
• Plant genome de novo sequencing: sequencing and assembling a plant
genome without any reference genome sequence. Next-generation
sequencing technologies allow researchers to move beyond model
organisms and gain an understanding of all plant genomes—a critical step in
unraveling the complexity of plants.
• Plant transcriptome sequencing: study the gene expression profiling at the
whole transcriptome level; also used to reduce the complexity of a large,
complex, and polyploid genome before sequencing entire genomes


Life Technologies solutions for plant genome
and transcriptome sequencing

de novo
de novo genome Small
genome Transcriptome sequencing genome Sequence
sequencing sequencing region confirmation
<1 Gb
>1 Gb sequencing
BAC clones


Ion PGM™ Sequencer: the fastest in the world
Key features
• Speed: 1.5 hour runs
• Scalability: 10 Mb to 1 Gb
• Simplicity: automated workflows,
benchtop convenience
• Affordable

Key plant genomics applications
• Plant de novo sequencing for genomes <1 Gb
• Plant transcriptome sequencing
• Genotyping by sequencing


The promise of semiconductor sequencing
First 100-fold scaling delivered and more

Achieved in 2011
Ion 318™ • 100-fold scaling and 200 bp kits,
Chip*
525-base perfect reads achieved
• Breakthrough Ion AmpliSeq™ Designer,
Ion 316™ microbial, and RNA-Seq apps
Chip
• 5,000 member Ion Community

Ion 314™ 2012 Roadmap
Chip
• 2 x 200 paired-end kit, 400 bp kits
• Custom and fixed Ion AmpliSeq™ Panels
• FDA submission and CE-IVD certification


Sedum album—small genome and
facultative CAM plant
• Sedum album, white stonecrop
− 142 Mb by flow cytometry
− 2n = 34
− Hart, 1991

• C3-CAM photosynthesis switching
− Facultative crassulacean acid metabolism (CAM) plant
− Under well-watered conditions, fixes carbon through
C3 photosynthesis (light)
− During drought, switches to CAM and fixes carbon at
night (dark)

Courtesy of Dr. Todd Michael, Monsanto, PAG Conference 2012, San Diego, USA

Genome and transcriptome analysis on the
Ion PGM™ Sequencer in <20 days*

Day 4–8 Day 11–13
Day 1 Extract RNA,
20 Ion runs
Buy plant sequencing
libraries

Day 2–3 Day 9–10
Extract DNA and Day 15–17
Assemble
make Ion library Transcriptome
genome
analysis

*Twenty noncontiguous days.

Courtesy of Dr. Todd Michael, Monsanto, PAG Conference 2012, San Diego, USA with minor modification

Genome assembly, gene calling, and annotation
• Error-correct raw reads (SAET)
• Remove any remaining adaptors
• Assemble using CLC

Avg stDev Min Max Range Median L50 (bp) N50 #Seq #Base

1,205 1,111 501 32,123 31,622 822 1,400 23,085 101,283 121,999,640

• Annotate using SNAP (against Arabidopsis) to improve the assembly


Transcriptome profiling: 1,183 genes significantly
differentially expressed

drought_only
drought_up_sig
water_up_sig
water_only

Drought_only: genes only expressed under drought condition, not detected under water
condition
Drought _up_sig: genes significantly up-regulated under drought condition
Water_up_sig: genes significantly up-regulated under water condition
Water_only: genes only expressed under water condition, not detected under drought
condition

Courtesy of Dr. Todd Michael, Monsanto, PAG Conference 2012, San Diego, USA with minor modification

Conclusions of the Monsanto Corporation
Sedum album sequencing project
• Ion PGM™ System provides a low-cost and robust platform for genome and
transcriptome discovery
• Sedum album genome is small; similar to the size of Arabidopsis
• Drought reduces cell wall gene expression
• FRIGIDA is up-regulated suggesting flowering suppression


De novo assembly of a 5 Mb algae genome
using an extra-long read sequencing protocol
Leptolyngbya sp. strain BL 0902 gDNA

Sheared into ~400 bp fragments by acoustic disruption

Sequencing library prep:
end-repairing sheared DNA, ligating Ion adaptors, sizing ~480 bp library fragments

Target enrichment with modifications to enable extra-long templating (400 bp)

Ion PGM™ Sequencing: Ion 316™ Chips

de novo assembly and data analysis

Conclusion: The >350 bp read protocol yielded a contig N50>16,000 bp (largest size
= 105,000 bp). The long read length enabled de novo assembly of this 5 Mb genome in a
single day.

Clancy et al. PAG Conference 2012, San Diego, USA

Coming soon—the Ion Proton™ Sequencer
The benchtop genome center

• Supports Ion Proton™ I and
Proton™ II chips: for any
plant genomes
− Proton™ I chip : 165 million
wells, up to 10 Gb data
− Proton™ II chips: 660 million
wells, up to 20x coverage of
human size genome

• State-of-the art electronics to
support highest throughput

The content provided herein may relate to products that have not been officially released and is subject to change without notice Life Technologies™ Corporation
19 6/28/2012 |

Unprecedented scale increases every 6 months


Plant Resequencing & SNP
Genotyping

Plant genotyping and SNP discovery
• SNP (single nucleotide polymorphism): A small variation in DNA sequences of a
genome. These variations can be used to track inheritance in families or
species.
• Plant SNP discovery: discover SNPs associated with desired traits to improve
or enhance certain characteristics such as higher yield or better stress
tolerance.
• SNP discovery and trait association study strategies
− SNP microarray arrays: identify informative SNPs in a collection of known SNPs
− Targeted resequencing: discover known SNPs and identify informative SNPs


Life Technologies SNP genotyping solutions
Research projects Best Life Technologies Why
approach platforms
Compare sequences of Genotyping Ion Proton™ and PGM™ • Speed, cost, scalability,
several crop variants to by sequencing simplicity, ease of use
discover functional SNPs (GBS)

Map up to 10 SNPs in Fragment 3500 Genetic Analyzer • Multiplexing capability:
different regions of the analysis up to 10 SNPs per reaction
genome • Gold standard
Map SNPs in a small Sanger • Accuracy, low cost
region of the genome sequencing • Simple workflow
Confirm putative SNP Real-time PCR QuantStudio™ • Easy and fast workflow
and develop SNP assays 12K Flex • Gold standard
• High call rate and accuracy
• Formats for different
project sizes (No. of SNPs x
No. of samples)


Targeted resequencing on the Ion PGM™ Sequencer

• The fastest sequencing runs and overall workflow
• Flexible solutions regardless of the size of the amplicon or target region
• Scalable for resequencing project needs, whether running single samples or
multiplexing


Genotyping by sequencing (GBS) in plants
• NGS has greatly increased SNP discovery in crop plant species such as rice, maize, soybean,
sorghum, and even in wheat’s predecessor, Aegilops tauschii
• GBS using next-generation sequencing technologies is becoming increasingly important:
• It is cost-effective
• It offers utility with complex genomes and those without a reference sequence
• GBS is a good approach for:
• Marker discovery
• Linkage mapping of QTL in a biparental cross
• Fine‐mapping QTL
• Bulked segregant analysis (BSA)
• Genome-wide association studies (GWAS)
• NAM‐GWAS
• Improving reference genome assembly


Advantages of genotyping by sequencing
• Faster, simpler protocol than traditional restriction-site-associated DNA (RAD)
method or full de novo sequencing
• Allows de novo marker (SNP) discovery, even in the absence of a reference
genome
• High accuracy of SNP calling
• Low cost
• Low amounts of input DNA needed
• Simplified computational analysis


Genotyping by Sequencing Strategies and
Workflows
• For unknown SNPs (discovery): restriction enzyme digestion is employed to
reduce complexity
• Known SNPs (screening): multiplexing PCR primers are designed and
barcoded to screen hundreds of SNPs in one sequencing run


GBS on Ion PGM™ Sequencer: a case study on barley

• Drs. Nils Stein (IPK, Germany) and Jesse Poland (USDA-ARS2, Manhattan,
KS, USA) partnered with Life Technologies to develop a protocol for GBS in
barley using two restriction enzymes

• The challenge: large, complex genome without complete sequence
available to date (~5.5 Gb, diploid)

• The goal: develop barley plants with improved traits (e.g., drought
tolerance, higher yield)
− Discovery of high-density molecular markers is required for better
understanding of genetics of complex traits for breeding
− Approach: genome-wide association (GWAS) studies and genomic selection


GBS approach for barley:
RE and Ion PGM™ Sequencer
• Restriction enzyme digestion of the genomic DNA to reduce complexity
− GBS targets the genomic sequence flanking restriction enzyme sites

• GBS is similar to RAD (restriction-site associated DNA) tagging but has greatly
simplified library construction that:
− Requires less DNA and avoids random shearing
− Is completed in two steps followed by PCR of the pooled library
• For barley, the original GBS protocol [1] was extended to a two-restriction-
enzyme approach [2]
• Completed a GBS feasibility study using the Ion PGM™ Sequencer

[1] Elshire et al. (2011) A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species.
PLoS ONE 6(5):e19379. doi:10.1371/journal.pone.0019379.

[2] Poland et al. (2012) Development of High-Density Genetic Maps for Barley and Wheat Using a Novel Two-Enzyme
Genotyping-by-Sequencing Approach. PLoS ONE 7(2):e32253. doi:10.1371/journal.pone.0032253.


Barley‘s GBS using the restriction enzyme approach
Simplified workflow chart
for GBS library preparation
using two restriction
1 enzymes for barley
1. Plant gDNA cleavage
using PstI and MspI for
desired restriction
fragments
2
2. Ligation of specific and
common adapters
3. Fragment
preamplification followed
3
by NGS on Ion PGM™
Sequencer


Barley GBS using Ion PGM™ Sequencer
• Feasibility study
• 4 barley samples; 2 parental, 2 F1-hybrids
• Sample prep using custom protocol
• Individual library preparation (previous slide)
• Multiplexed sequencing (barcodes)
− Ion PGM™ Sequencer, Ion 316 ™ Chip, 200 bp sequencing
• 2-day protocol
− Day 1: Library prep, template prep (Ion OneTouch™ System)
− Day 2: Enrichment (Ion OneTouch™ ES), Ion PGM™ Sequencing


Sequencing and SNP results in barley
• ~200 Mb Q20 sequence
− Approx. 500 k restriction fragments sequenced at 200 bp per sample
− 1-fold base coverage achieved in this study
• Good sample separation through barcodes
− >90% barcodes separated
− Barcode sequence followed by exact match to restriction site
• Roughly 5,000 SNPs per sample called
− SNP agreement >99.5% between Ion PGM™ Sequencer and Illumina® HiSeq®
System (NGS platform previously used by collaborator)
− Customer statement: “Concordance is as high as between runs on our platform”
• Technical feasibility acknowledged


Data analysis
Two independent approaches used
• KSU: TASSEL pipeline
• Life Technologies
− Mapping/Alignment
> Torrent Suite Software v2.1; TMAP (Torrent Mapping
alignment program)
> Input is SFF file format, output is SAMtools BAM file format
− SNP calling
> SAMtools* mpileup
(http://samtools.sourceforge.net/mpileup.shtml)
> Output is ‘variant call format’ (VCF)

*The Sequence Alignment/Map (SAM) format and SAMtools:
bioinformatics.oxfordjournals.org/content/early/2009/06/08/bioinformatics.btp352


Conclusions and outlook
• Promising results led to an extended study (phase 2; in progress)

• Design:
− Increased sample number (two 24-plex pools)
− Increased coverage for higher SNP-counts per sample
> Ion 318™ Chips, 200 bp sequencing
− Comparison of Life Technologies sample prep solutions with customer protocol

• Data to be compared to Illumina® HiSeq® results

• Ion semiconductor sequencing has huge potential for large GBS studies:
− High SNP calling accuracy
− Highly competitive cost per sample
− Unmatched sequencing workflow speed Learn more
lifetechnologies.com/agbio
lifetechnologies.com/gbs


QTL Mapping & Marker-Assisted
Selection

Marker-assisted selection (MAS)
• Useful in early generations
• Ability to select for recessive alleles
• Fast and cost-effective
X
A

A A A A A
New variety
A
Cycles of breeding

Gene of interest
Eliminate
A Marker linked to gene X
individuals
without marker

36
Courtesy of Jochum Wiersma, U. Minnesota Extension 6/28/2012 | Life Technologies™ Corporation

Life Technologies solutions for QTL mapping and
marker-assisted selection
Number of SNPs
in the project*
5,000 Genotyping by
sequencing (GBS) on
Ion PGM™ System
•Low cost for total project
•Fast and easy workflow
500 •Flexibility
•Affordability
TaqMan® Assays on QuantStudio™
OpenArray®
•Low cost
•Proven TaqMan® chemistry
•Streamlined workflow
•Reduction in consumables and steps
100
TaqMan® Assays, HRM, SSRs TaqMan® Assays on Douglas Array Tape™
•Fast and Easy workflow •High throughput
•Gold standard with high call rate
10 and accuracy (TaqMan®)
•Automated workflow
•Low cost
•Very low cost (HRM) •Proven TaqMan® chemistry

10 100 500 1,000 5,000
Number of samples
in the project**
* number of SNPs or other markers in a design/panel
** number of samples screened using a design/panel


Life Technologies solutions for QTL mapping and
marker-assisted selection


Examples of publications using TaqMan® SNP and SSR
genotyping assays for MAS


Designing TaqMan® assays for crops with
unknown genomes: canola example

• Canola (Brassica napus): a tetraploid
crop converged from diploid Brassica
rapa and Brassica oleracea
• Canola genome has not been sequenced
• Assay design pipeline predicted
propensity to cross hybridize to
nontarget loci in the genome by
mapping the assays to B. rapa
www.nrc-cnrc.gc.ca/eng/news/pbi/2011/08/28/brassica.html
and B. oleacera
• Genome cross-hybridization analysis
increased the success rate by 30%

Brzoska, et al. Plant and Animal Genomics. San Diego, CA.2011.

TaqMan® OpenArray® on QuantStudio™12K Flex
Real-Time PCR System
• Fast and simple workflow: 4 hours from DNA to genotyping call
• High sample throughput: screen up to 256 SNPs across >1,500 samples
(>70k data points) in one day without the use of robotics
• Low cost per data point
OpenArray® flexible formats
Assays Samples
16 144
32 96
64 48
128 24
192 16
256 2

Wrong, et al. Maize Genetics Conference, Portland, OR. 2012

High-throughput SSR genotyping by HRM
• The goal: develop an accurate SSR genotyping approach using HRM that is compatible
with high-throughput breeding programs in Jatropha curcas
• Subtropical plant that produces a high-quality oil for biodiesel, renewable jet fuel, or specialty products

• Low genetic variation in geographic regions outside Central America (where the plant
originated)
• HRM assays (MeltDoctor™ HRM Master Mix) and ViiA™ 7 Real-Time PCR System were
used to discover remarkable genetic diversity in the SG Biofuels germplasm collection

• Results
− High allelic polymorphism of SSR26 in
the SG Biofuels germplasm collection
− Among 380 unique accessions, 9
alleles and 24 different genotypes
were detected by HRM and
confirmed by sequencing

Download the application note here

Genetically modified crops: facts and regulations
• Genetically modified (GM) crops: first cultivated on a commercial scale in 1996;
major GM crops include soybeans, corn, alfalfa, canola, and cotton
• GM crops are grown on 1/10 of total cultivated land globally (170 million acres) and are
expected to expand to 20 million farmers in 40 nations by 2015
• GMO testing in seed, grain, and processed food and their ingredients is required in many
countries
− Amount of GM ingredients that can be present in a food product without being labeled as
“GM” is 0.5% in EU and 5% in Japan

Agricultural Biotechnology - A Global strategic business
report. Global Industry Analysts, Inc. 10/2010
Map (2008 view):
sustainablelinfield.edublogs.org/files/2011/05/Picture-1-
1y5b0mr.png


Technologies for GMO testing and detection
• Digital PCR on QuantStudio™ 12K Real-Time PCR System for:
− Contamination or GMO detection
− Rare mutation detection

• TaqMan® Real-time PCR assays
• Dedicated TaqMan® GMO kits:
− TaqMan® GMO Maize 35S Detection
− TaqMan® GMO Soy 35S Detection


GMO detection of maize endogenous reference genes
using TaqMan® Real-Time PCR Assays
• Research goal: select the best TaqMan® Assay and real-time PCR condition to detect GMO maize
• Approach:
− Five TaqMan® real-time PCR assays targeting adh1 and hmg genes were designed and
tested using different amplification profiles on the 7900HT system
− Equal amounts of DNA from 7 EU-certified maize flours were pooled
− Both pooled and single DNAs were serially diluted 8 times

• Conclusion: TaqMan® Real-Time PCR assay targeting maize endogenous reference genes can be
used for the quantification of transgenic events in Zea mays

Relative standard deviation (RSD)% over the
concentration range
Assay met4 (hmg) demonstrated the best
regression parameters and a higher repeatability
over the dilution rage

PATERNO` et al. J. Agric. Food Chem. 2009, 57, 11086–11091

Contaminant detection in soybean by dPCR on
the OpenArray® System

Digital PCR workflow Spike-in simulation of seed contamination

• 6 allele-specific TaqMan® SNP Assays were designed and validated for distinguishing
soybean strains
• Achieved a detection sensitivity of 1:10,000 contaminant variety B in
variety A soybean seed DNA background
• The dPCR approach is an ideal solution for GMO testing and other AgBio solutions

Webster et al. Plant and Animal Genomics, San Diego, 2012

Other Products & Applications
Plant Genetic Engineering
Gene Expression Analysis
Plant DNA and RNA Isolation Products

Plant genetic engineering as a tool for plant research

Plant transformation and
regeneration of transgenic plants is a
key approach for plant research:
− Understanding gene expression
and regulation

− Decipher metabolic and signal
pathways

− Developing plants with new
characteristics

What are the challenges?
− Manipulation of DNA elements

− Effective plant transformation
and tissue culture techniques

− Achieve desirable gene
expression levels and ultimately,
the desired plant phenotype
A basic primer on biotechnology. Dr. Peel, NDSU Extension, October 2011.


Plant genetic engineering solutions
If you want to do your own cloning
• Plant DNA cloning and site-directed mutagenesis kits
• Competent E.coli cells and transformation
• Custom DNA oligonucleotides
• PCR enzymes and thermal cyclers
If you want us to do the cloning for you
• Cloning services
• GeneArt® gene synthesis services
Plant transformation GeneArt® Chlamydomonas Engineering Kits

• Agrobacterium tumefaciens LBA4404 competent cells
• Antibiotics: carbenicillin, kanamycin, hygromycin B

Genome engineering
• GeneArt® Precision TALs NEW


GeneArt® Precision TALs—our new service for
genome editing
Custom DNA binding proteins for precision DNA targeting
What are they used for?
• Gene targeting (Fok1 nuclease pair)
• Silencing
• Incorporation of exogenous DNA
• Activation (activator vp16 or vp64)
• Increasing the expression level of endogenous gene
isoforms
• Effector domain targeting (MCS vector)
• Target any locus in the genome with the effector
domain of
your choice with our multiple cloning site vector


TAL effector technology
How TALs function
• Bacterial pathogen proteins redirect transcription
of host plants upon infection
• TAL proteins use discrete domains to recognize A, T,
G, and C nucleotides in dsDNA

Engineered system
• Modular assembly of domains allows for creation
of sequence specific DNA binding proteins

Why is this technology so compelling?
• Simple code for creating engineered TAL proteins:
no bias except for a 5’ T
• More predictable than Zn fingers
• One-to-one correspondence between the identity
of two critical amino acids in each repeat and each
DNA base in the target sequence


GeneArt® Precision TALs—ordering
•Access the GeneArt® web portal from the Life
Technologies website

•Download and complete the order form

•Email the completed form to
geneartsupport@lifetech.com
•All inquiries will be answered within 24
hours
•Production starts within 24 hours of
ordering
•3 weeks from order to deliver

QC
1) Submit preverification
2) Intermediate assembly sequence
3) TAL terminus sequence
4) TAL size

53


Gene expression analysis

RNA-Seq for whole transcriptome sequencing

Targeted mRNA expression using NCode™ miRNA qRT-PCR Kits
TaqMan® Assays


Plant DNA isolation solutions
Total RNA TaqMan® Plant DNAzol® ChargeSwitch® PureLink® MagMAX™
Lysis Sample-to- Reagent gDNA Plant Kit Genomic DNA Multi-
Solution SNP™ Kit Plant Kit Sample Kit
Key features Bulk buffers Extraction+ Most efficient Most suitable Low Low
for crude TaqMan® for large for GMO abundance abundance
extraction Assay amounts of testing DNA samples DNA samples
combined tissue
Downstream PCR, real- Real-time All All All All
applications time PCR PCR
Protocol time <15 min 5 min <60 min <15 min <40 min <40 min

Starting ≥0.1 g 2–3 mm ≥0.1 g 50–100 mg 100 mg 5–10 mg
materials punch
Yield Varies Varies Varies Up to 7 µg Up to 14.6 µg Up to 14.6
µg

Isolation Bulk lysis Lysis Organic ChargeSwitch® Silica spin Magnetic
technologies buffer solution extraction + magnetic column beads
beads
Automatable No Yes No Yes No Yes

High- Yes Yes Yes Yes No Yes
throughput


Plant RNA isolation solutions

Plant RNA Reagent MagMax™-96 RNA PureLink® RNA mirVana™ miRNA
Isolation Kit Mini Kit Isolation Kit
Key features Great for difficult Rapid and fully Quick and easy to Efficient recovery of
samples (conifer automated use miRNA and small
tissue and seeds) RNA
Protocol time 60 min <45 min <20 min 30 min

Starting materials Up to 1 g Up to 10 mg <50 mg 0.5–200 mg

Isolation Organic extraction Magnetic beads Silica column Organic extraction
technologies and silica column
High-throughput No Yes No No
compatible


Full-spectrum genetic analysis and beyond
Solutions in plant agriculture biotechnology
Plant RNA & DNA isolation and purification

Genome sequencing Targeted sequencing Marker-assisted selection Plant genetic engineering
Transcriptome sequencing Sequence confirmation SNP confirmation Gene synthesis
Genotyping by sequencing SNP confirmation HRM genotyping Genome editing
SNP discovery Microsatellite/SSR analysis GMO testing (TAL effectors)
QTL mapping Marker-assisted selection Sample QC Plant DNA cloning
Marker-assisted selection QTL analysis Rare allele detection Plant transformation
Targeted gene expression Mutagenesis
miRNA analysis

Discover Confirm and screen Engineer


One globe, one team, one goal
Questions or comments? Looking for custom solutions?
---- We are always here to support you
• Contact us at agbio@lifetech.com
• Visit us at www.lifetechnologies.com/plants

For Research Use Only. Not for use in diagnostic purposes.
© 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of
Life Technologies Corporation and/or its affiliate(s) or their respective owners.


Plant Agriculture Biotechnology: Empower Your Plant Science Research

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