Current Status of TILLING and EcoTILLING:
TILLING and EcoTILLING technique have been adapted in diverse species including rice, maize, Lotus, poplar, Arabidopsis, wheat, barley, potato, tomato, sunflower, common bean, Field Mustard, clover, melon, pea, peanut, sorghum, rapeseed, soybean, melon, poplar, sugarcane, brassica and other for the purpose of gene detection, functional genomics, polymorphism assessment, plant breeding as described in case study part.
Ecotilling:
EcoTILLING is similar to TILLING, except that its objective is to identify natural genetic variation as opposed to induced mutations.
Many species are not amenable to chemical mutagenesis; therefore, EcoTILLING can aid in the discovery of natural variants and their putative gene function
This approach allows one to rapidly screen through many samples with a gene of interest to identify naturally occurring SNPs and / or small INs/DELS
iTILLING:
A new approach to the TILLING method that reduces costs and the time necessary to carry out mutation screening was developed for Arabidopsis and it is called iTILLING, individualized TILLING
21. TILLING and EcoTILLING technique have been adapted in
diverse species including rice, maize, Lotus, poplar,
Arabidopsis, wheat, barley, potato, tomato, sunflower,
common bean, Field Mustard, clover, melon, pea, peanut,
sorghum, rapeseed, soybean, melon, poplar, sugarcane,
brassica and other for the purpose of gene detection,
functional genomics, polymorphism assessment, plant
breeding as described in case study part.
Current Status of TILLING and
EcoTILLING
22. EcoTILLING
• EcoTILLING is similar to TILLING, except that its
objective is to identify natural genetic variation
as opposed to induced mutations.
• Many species are not amenable to chemical
mutagenesis; therefore, EcoTILLING can aid in the
discovery of natural variants and their putative
gene function
• This approach allows one to rapidly screen
through many samples with a gene of interest to
identify naturally occurring SNPs and / or small
INs/DELS
24. iTILLING
• A new approach to the TILLING method that
reduces costs and the time necessary to carry
out mutation screening was developed for
Arabidopsis and it is called iTILLING,
individualized TILLING
