Genetic engineering, also called genetic modification, is used to add new traits to organisms that do not naturally occur. The document discusses various applications of genetic engineering including in agriculture, medicine, and genetic studies. In agriculture, genetic engineering has been used to develop crops with traits like virus resistance, insect resistance, and herbicide tolerance. In medicine, genetic engineering is being used to produce human insulin, growth hormone, vaccines, and interferons. It is also being explored for gene therapy applications.
Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.https://www.creative-biolabs.com/phage-display-service.html
Application of Biotechnology In Medicine By Anila Rani Pullaguraanilarani
Biotechnology is a very huge field and its applications are used in a variety of fields of science such as agriculture and medicine. Medicine is by means of biotechnology techniques so much in diagnosing and treating dissimilar diseases. It also gives opportunity for the populace to defend themselves from hazardous diseases.
Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies.
Application of Biotechnology In Medicine By Anila Rani Pullaguraanilarani
Biotechnology is a very huge field and its applications are used in a variety of fields of science such as agriculture and medicine. Medicine is by means of biotechnology techniques so much in diagnosing and treating dissimilar diseases. It also gives opportunity for the populace to defend themselves from hazardous diseases.
Synthetic biology is the designing of new biological systems or the modification of the existing ones that do not occur naturally. Synthetic or artificial cells organisms with minimal genomes have uses in molecular medicine, vaccines, environmental chemistry and bio-sensors. Creation of synthetic cells involve in-vitro synthesis of unitary DNA fragments of one-kilo base pairs (1kb). These unitary fragments are ligated to make ten kilo base pair (10kb) fragments, followed by tethering 10 fragments to form one hundred kilo base pair (100kb) fragments. Each step involves transformation and sequencing procedures in E. coli host cells. Ultimately, eleven of these hundred kilo base pair fragments are joined to create a “Synthetic Genome” which is maintained in yeast cells, as maximum limit of DNA transplant acceptance of E. coli is 100kb. By this approach, synthetic chromosomes can be maintained, manipulated and transplanted to an acceptor organism to create a synthetic cell. Applications of the technology include semi-synthetic approach of Artemisinic acid, which can be used to chemically synthesize anti-malarial drug Atremisinin and its therapeutically important derivatives. Second application of synthetic biology is production of meningitis vaccine against poorly immunogenic Neisseria meningitidis serogroup-B, by preparing synthetic vesicles. Third application includes disease mechanism identification of a rare-primary immunodeficiency disease “Agamaglobinemia” using reconstruction of mutant B-cell receptor components in synthetic membranes to validate a point mutation. Fourth application include environmental fixation of carbon di-oxide to produce methane by using minimal genome containing synthetic cells of Metahnococcous sp. Fifth application is production of novel biosensors which can be toggled ON and OFF using “Visible Light” as modulator. These “Gene switches” are also able to operate in mammalian cells. With potential applications and wide research domains, synthetic biology is also under ethical and religious criticism. Future of this new dimension of biological science requires scrutiny from regulatory authorities, and monetary input from funding agencies.
Advanced Oxidation Process for Industrial Water Treatment and Waste WaterUus Soedjak
Advanced Oxidation Process for Industrial Water Treatment and Waste Water is a liquid waste treatment technology which utilizes oxidation method using ozone gas. This technology is combined with ultraviolet light
Genetic Engineering of Bacteria for a Microbial Fuel Cellmwporter2
This presentation goes over the basic concept of a Microbial Fuel Cell (MFC), the challenges of MFC efficiency, and the genetic approach to engineering bacteria to address these challenges.
This presentation gives an brief idea about the applications of genetic engineering which is of at most importance to humans. Provided along with this slide is an example which makes it easier to understand the concept.
CHAPTER 12 BIOTECHNOLOGY AND ITS APPLICATIONS.pptxJyoti Gadge
This PPT explores many aspects of applications of biotechnology. Learn about the Biotechnological applications in agriculture,
Biotechnological applications in medicine,
Transgenic animals and
Ethical issues. Easy-to-understand explanations. Whether you're a student, a teacher, or just someone who wants to learn more about applications of biotechnology, this PPT is a must-see.
This presentation highlights some important facts about biotechnology in relationship to plants. it lay emphasis on some factors associated with biotechnology, the importance of it and the negative impact as well.
Applications of rdna technology in medicinesAdarsh Patil
Applications of R-DNA Technology in medicines:
Introduction Steps involved in recombinant technology:
DNA fragments coding for proteins of interest are synthesized chemically or isolated from an organism.
These DNA fragments are inserted into an endonuclease cleavage site of the vector that does not inactivate any gene that is required for the vector’s maintenance and selective marker.
The recombinant DNA molecules are then introduced into a host to replicate using the replication origin of the vector.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
This pdf is about the Schizophrenia.
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3. • Genetic engineering, also called genetic
modification
• to add one ormore new traits that are not
already found in that organism.
• In France , this technology is called
biomolecularengineering
• In 1973,Stanley Cohen and Herbert Boyer
designed a methodology fortransferring
certain genes fromone organismto another
4. • The gene is a small piece of DNA that encoded for
a specific protein
• A desired gene is inserted in to a vectorDNA so
that a new combination of vectorDNA is formed
• The DNA formed by joining DNA segments of
two different organisms is called recombinant
DNA orchimeric DNA
• The gene is introduced in to a cell in the formof
recombinant DNA
• The gene manipulation therefore is known as
recombinant DNA technology
5. • The organism whose genetic make up is manipulated
using recombinant DNA technique , is called genetically
modified organism (GMO).
• GMOs produced through genetic technologies have become
a part of everyday life,
6. • However, while GMOs have benefited human
society in many ways
• Genetic engineering techniques have been
applied in numerous fields including :-
Agriculture
Medicine
Genetic studies . etc
• some disadvantages also exist; therefore, the
production of GMOs remains a highly
controversial topic in many parts of the world.
7. Application in Agriculture
• Improvement in agricultural production and the
food and nutrition situation depents on land
,waterand energy resources.
• From1970 a new type of reasearch started with
the aimof producing new varieties of plants by
genetic recombination techniques.
• They are genitically engineered plants
• They have acquired a new trait fromthe
introduced DNA and inherit the trait formany
generations
8. • The new plants produced by such techniques are
supposed to be:-
virus resistant plant
insect resistant plants,
herbicide resistant plant
• growing crops of yourchoice (GMfood)
• forpreservation of fruits
• Some genetically engineered microorganisms are used
as nitrogen fixers
9. virus resistant plant
• to make virus resistant crops. The most
common way of doing this is by giving
a plant a viral gene encoding the virus'
'coat protein'.
• The plant can then produce this viral
protein before the virus infects the
plant.
• When the virus tries to infect the plant,
the production of its essential coat
protein is already blocked.
10. • Tansgenic tobacco is developed by introducing gene coding
forcapsid protien of tobacco mosaic virus
• TMV-coat protein inserted in to tobacco cell using Ti plasmid
• Viral capsid inhibit viral replication of TMV when infected
• All genetically modified virus resistant plants on the market
(e.g. papayas and squash) have coat protein mediated
resistance.
11. GMsquash with virus
resistance (top) protects the
squash from the damaging
effects of the virus (bottom) papaya with virus resistance
12. insect resistant plants,
• Insect attackis a serious agricultural problem leading
to yield losses and reduced product quality.
• Each year, insects destroy about 25 percent of food
crops worldwide.
• many transgenic plant with insect resistantance have
been developed by adopting gene transfermethods
• The gene helps the plants produce proteins that are
toxic to certain insects
• They reduced the use of chemical pesticides in
agriculture
13. • The genetically modified crops is called Bt-
crops, because the insect-killing gene in the
plant comes fromthe bacteria Bacillus
thuringiensis.
• Bt gene produces insecticidal crystal protien
(ICP) also known as cry protien which is in an
inactive, crystalline form.
• When consumed by insects, the protein is
converted to its active, toxic form(delta
endotoxin), which in turn destroys the gut of
the insect and is completely safe forhumans.
14. Examples of some Bt crops
A common soil bacteriumso
called because it was first
isolated in the Thuringia region
of Germany
discoverd by Ishiwatari in 1901
15. herbicide resistant plant
• Many transgenic plants with herbicide resistance have
been developed by using genetic engineering
• Such transgenic plants tolerate the herbicides and be safe
in the field ,when the herbicides are applied in the field
17. Growing crops of yourchoice
• Genetically modified foods are foods derived from
genetically modified organisms.
18. • The FLAVRSAVRtomato was the first genetically
engineered crop product
• Through genetic engineering, the ripening process of the
tomato will slow down and thus prevent it from
softening, while still allowing the tomato to retain its
natural colourand flavour.
• Thus the shelf life of the tomato become improved
19. • The tomato was made more resistant to rotting by
adding an gene which interferes with the production
of the enzyme polygalacturonase.
• The enzyme normally degrades pectin in the cell walls
and results in the softening of fruit which makes them
more susceptible to being damaged by fungal
infections.
• Improved taste and look
20. Biofertilizers
Genetically engineered nitrogen fixers
• Nitrogen is the most essential macro-element for
properhealthy crop
• Molecularnitrogen in the atm. Is converted to
biologically usable forms by nitrogen fixing micro
organisms eg:Rhizobium
• In leguminous plants nitrogen fixing nodules are
formed in the roots due to the symbiotic realation ship
with bacteria
• The special ability of nitrogen fixing bacteria is due to
the presence of an enzyme called nitrogenase complex
21. • Some genetically engineered micro organismare used
as nitrogen fixers
• rizobium species carry nif genes , latergenes will
transferred to the free living bacteris like klebsiella
pneumoniae ,salmonella typhimuriumetc
• These genetically engineered microbes are capable of
fixing the atmospheric nitrogen in the soil they are used
as biofertilizers in agriculture.
22. • In 1971 dixon and postgate successfully transferred
the nif genes to free living nitrogen fixerklebsiella
pneumoniae
• Klebsiella is naturally found as a free-living soil
bacterium
23. Application in
Medicine.• Some of the most promising and powerful
applications of genetic engineering are in the
field of medicine.
• Researchers are using it to diagnose and predict
disease, and to develop therapies and drugs to
treat devastating diseases like cancer,
Alzheimer's, diabetes, and cystic fibrosis. Etc.
• At present about 30 recombinant therapeuties
have been approved forhuman use
24. • The application of recombinant DNA technology
has played a majorrole in :-
Production of human insulin
Production of human growth
hormone
Production of vaccines
production of interferons
Gene therapy
25. Production of human insulin
• Insulin is a hormons produced by beta cells in
the pancreas that controls the absorption of glucose
by cells.
• The deficiency of insulin leads to diabetics in man
• Diabetics is treated by injecting insulin
• In the past, diabetics needed to take insulin purified
frompigs and cows to fulfill theirinsulin requirement.
However, non-human insulin causes allergic reactions
in many diabetics.
26.
27. • Recombinant DNA technology has
allowed the scientists to develop
human insulin by using the bacteria
as a host cell
• it is also available in the market. It is
believed that the drugs produced
through microbes are safer.
28. Production of human growth
hormone
• Human growth hormone (HGC) is secreted by the anterior
lobe of pituitary gland . It also called somatotropin
• The deficiency of somatotropin leads to dwarfismin man
• The cDNA of somatotropin was introduced in to Ecoli The
genetically engineered E coli produces human growth
hormone
29. Production of vaccines
• A vaccine is a biological preparation that improves
immunity to a particular disease
• Genetic engineering is used to developed vaccines
against some severdiseases
• Hepatitis Bvaccines, First human trials of vaccine
against Hepatitis B
• A gene coding forsurface antigen HBsAg was isolated
from Hepatitis Bvirus and cloned in Ecoli
• The genetically engineered microbes will produced HBs
antigen
• This antigen is isolated purified and used for
vaccination
30. • Edible Vaccine involves introduction of selected
desired genes into plant and then inducing
these altered plants to manufacture the altered
protein.
• Banana is currently being considered as the
edible vaccine against hepatitis B
31. Production of interferons
• Interferons is an antiviral protein.
• Interferons is produced by infected animal cells
and it protects the cells fromthe second viral
infection
• They induced the production of cellularprotein
kinase and phosphodiesterase which selectively
destroy viral RNAs and proteins. So the virus
fail to multiply in the cell
32. • The cDNA of the various interferons were isolated
from human cell and introduced in to E coli
• The genetically engineered E coli cultures
produced interfrons
33. Gene therapy
• Medical scientists now know of about 3,000 disorders
that arise because of errors in an individual's DNA.
• Conditions such as sickle-cell anemia, Huntington's
chorea, cystic fibrosis, and Lesch-Nyhan syndrome are
the result of the loss, mistaken insertion, orchange of a
single nitrogen base in a DNA molecule.
• Genetic engineering makes it possible forscientists to
provide individuals who lacka certain gene with correct
copies of that gene.
34. • Treatment of genetic disorders by replacing
defective gene by a normal functional gene is
called gene therapy
• The gene used in gene therapy is often called
gene drugs
• The drug gene may be introduced in to somatic
cells orgerms cells orzygotes .
• If the genes are introduced into somatic cell it
is called somatic cell gene therapy
• If genes are introduced into eggs orzygotes it is
called germline gene therapy
35. • The gene drug may be introduced in to target
cells by using retrovirus, electroporation
,transfection etc
• The first clinical gene thearapy was given in
1990 to a 4 yearold girl with adenosine
deaminase deficiency
36. Application in Genetic
studies
• Today recombinant DNA technology is used
extensively in research laboratories
worldwide to explore myriad questions
about gene structure, function, expression
pattern, regulation, and much more.
37. • The most common application of recombinant DNA is in
basic research, in which the technology is important to
most current workin the biological and biomedical
sciences.[8
• Recombinant DNA is used to identify, mapping genes
sequence, and to determine theirfunction.
• Mapping helps in finding the inheritance of many rare
genetic disorders such as cystic fibrosis, haemophilia etc.
• It helps in understanding the expression and regulation
of a commercially important trait.
38. ]
• rDNA probes are employed in analyzing gene
expression within individual cells, and
throughout the tissues of whole organisms.
• Recombinant proteins are widely used as
reagents in laboratory experiments and to
generate antibody probes forexamining protein
synthesis within cells and organisms.