This document provides an overview of biotechnology. It begins by defining biotechnology as using living things to make useful products. It then discusses the history and development of biotechnology. The document outlines several types of biotechnology including green (agriculture), red (medical), white (industrial), and blue (aquatic) biotechnology. It provides examples of how each type is used. The document also discusses uses of biotechnology in medicine, agriculture, environmental applications, cloning, and genetic testing. It defines key terms like bacterium and explains the process of DNA replication and how genes are transferred.
Hi all! I used different references for this. The link for pros and cons is here.
Reference for pros and cons : https://vittana.org/11-biotechnology-pros-and-cons
biotechnology and its applications
application s of biotechnology, bt.cotton, cloning, dna, dna fingerprinting, dna isolation, gene manipulation, genetic engineering, goldenrice., r dnatechnology, recombinant vaccines, transgenic, vectors
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
Hi all! I used different references for this. The link for pros and cons is here.
Reference for pros and cons : https://vittana.org/11-biotechnology-pros-and-cons
biotechnology and its applications
application s of biotechnology, bt.cotton, cloning, dna, dna fingerprinting, dna isolation, gene manipulation, genetic engineering, goldenrice., r dnatechnology, recombinant vaccines, transgenic, vectors
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
Some of the landmark discoveries are tabulated below: 1902 Haberlandt proposed concept of in vitro cell culture 1966 Guha and Maheshwari produced first haploid plants from pollen grains of Datura
1904 Hannig cultured embryos from several cruciferous species 1970 Smith and Nathans discovered first restriction enzyme from Haemophilus influenza (HindIII)
1922 Kolte and Robbins successfully cultured root and stem tips respectively 1970 Baltimore isolated Reverse transcriptase from RNA tumour virus
two dimensional gel electrophoresis system
History of Genetic Engineering
Tools of Genetic Engineering
Principles of rDNA technology
Applications of Genetic Engineering in agriculture medicine and orthodontics
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
this helps to understand the normal techniques related to biotechnology in a simple manner and provides you broad idea about the subject. A brief knowledge about the topic is presented in this presentation.
Some of the landmark discoveries are tabulated below: 1902 Haberlandt proposed concept of in vitro cell culture 1966 Guha and Maheshwari produced first haploid plants from pollen grains of Datura
1904 Hannig cultured embryos from several cruciferous species 1970 Smith and Nathans discovered first restriction enzyme from Haemophilus influenza (HindIII)
1922 Kolte and Robbins successfully cultured root and stem tips respectively 1970 Baltimore isolated Reverse transcriptase from RNA tumour virus
two dimensional gel electrophoresis system
History of Genetic Engineering
Tools of Genetic Engineering
Principles of rDNA technology
Applications of Genetic Engineering in agriculture medicine and orthodontics
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
this helps to understand the normal techniques related to biotechnology in a simple manner and provides you broad idea about the subject. A brief knowledge about the topic is presented in this presentation.
This presentation covers the discussion of Biotechnology. Biotechnology is the integration of natural sciences and engineering sciences in order to achieve the application of organisms, cells, parts thereof and molecular analogues for products and services.
CHAPTER 12 BIOTECHNOLOGY AND ITS APPLICATIONS.pptxJyoti Gadge
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Slide Presentation: Introduction to Plant Biotechnology
Slide 1: Introduction to Biotechnology
Definition of biotechnology
Importance and relevance of biotechnology in various fields
Slide 2: Historical Development of Biotechnology
Key milestones in the development of biotechnology
Contributions of notable scientists and researchers
Evolution of biotechnological techniques and methodologies
Slide 3: Branches of Biotechnology
Overview of different branches of biotechnology
Genetic engineering and its role in plant biotechnology
Other branches such as agricultural biotechnology and medical biotechnology
Slide 4: Applications of Biotechnology
Introduction to various applications of biotechnology
Agricultural applications: crop improvement, pest control, and disease resistance
Medical applications: drug development, gene therapy, and diagnostics
Slide 5: Biotechnology in Ethiopia
Overview of the biotechnology landscape in Ethiopia
Government initiatives and policies supporting biotechnology
Examples of successful biotechnology projects in Ethiopia
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3. • Define Biotechnology
• Describe applications of biotechnology
• Describe the uses of biotechnology in daily
life
• Define bacterium
• Explain the process of DNA replication
• Describe how DNA is copied
OBJECTIVES
4.
5. WHAT IS BIOTECHNOLOGY?
Biotechnology is branch of science in which
living things are used to make new
products.
• It is the use of living organisms or
processes to make products useful for
mankind.
6. • Has been existing since centuries
• Begin with the first action of human on life
for his welfare
• It started when people modified
microorganisms to the needs of humanity.
• In past biotechnologists used yeast cells to
ferment alcoholic drinks and the bacteria to
make cheese and yoghurt.
HISTORY
7. Term coined by a Hungarian engineer Karl Ereky
Modern biotechnology started in California in
1970’s
Before 1971, the term biotechnology was used
primarily in food processing and agriculture
industries.
8. SCIENCE BULLETIN - BIP
Biotechnology industrial park (BIP) is a special form
of industrial park specialized in biotechnology.
Such industrial park usually hosts a cluster of
companies that work together as a bio-refinery. A
bio-refinery is a biomass equivalent of an oil
refinery. These companies refine bio materials and
produce bio-products.
15. • Green biotechnology (agricultural applications)
• Red biotechnology (medical applications)
• Blue biotechnology (aquatic applications)
• White biotechnology (industrial applications)
TYPES OF BIOTECHNOLOGY
18. • Use of biotechnological techniques in agriculture
• Vitamin A deficiency is a serious problem and can
cause blindness at a young age if left untreated
• Golden rice was genetically modified to produce
beta-carotene (a precursor of vitamin A that the
body converts to vitamin A). A diet including
golden rice can thus help to raise vitamin A levels
GREEN BIOTECHNOLOGY:
20. WHITE BIOTECHNOLOGY
• It is also called industrial biotechnology. It
is the designing of an organism to produce
useful chemicals i.e enzymes
21. • The use of biological methods to optimize industrial
processes
• Applied by manufacturers of laundry detergents
• Includes research for new enzymes (proteins that
remove oily and protein-based stains)
• Enzymes that work under extreme conditions (wash
temperatures of 20°C or 90°C)
• This often entails modifying the enzymes of
microorganisms for these processes
WHITE BIOTECHNOLOGY
22. This term is used in medical processes. It
involves the manipulation of microorganisms
to make products such as antibiotics.
RED BIOTECHNOLOGY
23. • It includes:
o Production of medicines and pharmaceutical
products for treating or diagnosing disorders
o Designing of organisms to manufacture
antibiotics and vaccines
o Engineering of genetic defects through
genomic manipulation
o Use in forensics through DNA profiling
RED BIOTECHNOLOGY
24. • Production of human insulin from non- human
sources.
• Production of hormones like Interferons,
Cytokinins, Steroids and human growth
hormones.
• Gene therapy for prevention and control of
diseases like hemophilia cystic fibrosis
• Development of vaccines and antibodies for
rabies, HIV, etc.
EXAMPLES…
26. DEVELOPMENT OF MEDICINE
With the help of biotechnology:
•Microorganisms can be used to make biological substances
for humans which are equally effective as natural substances
•Microorganisms such as bacteria and yeast are used to make
substances (produce drugs) like insulin and interferon
•Treatment of diseases like hepatitis and arthritis are made
possible with the help of biotechnology
27.
28. • INSULIN:
Human insulin is being produced using genetic
engineering technique known as humulin and it is
used for the treatment of diabetes that is low sugar
level in the blood…..
DRUG PRODUCTION
29. • INTERFERON:
o Interferon interfere in transmission of viral genome from
one cell to another and it also inhibits the cell division of
abnormal cells.
o Interferon produced using the recombinant DNA
technology is used to treat cancer patients.
o Interferon improved the quality of life of cancer
patients…..
DRUG PRODUCTION
30. • HUMAN GROWTH HORMONE:
Since dwarfism is caused by growth hormone
deficiency so it can be diagnose by HGH testing.
So HGH is used for the treatment of dwarfism due to
hypo pituitary activity.
DRUG PRODUCTION
31.
32.
33. • Replacing a mutated gene that causes
disease with a healthy copy of the gene
• Inactivating or “knocking out” a mutated
gene that is functioning improperly
• Introducing the new gene that help fight a
disease
GENE THERAPY
34. • Some common ways are:
• Using fat droplets in nose sprays
• Using cold viruses that are modified to
carry alleles ,go into the cell and affect
them
• The direct injection of DNA(might include
GENE THERAPY
35. • It involves the direct examination of DNA
molecule of an organism. It is used for
• Newborn screening: Test for abnormal
missing of gene product
• Carrier Testing: Test to avoid transfer of
defective gene from parents to child
• Identity Testing
• Genetic disorder like
Sickle cell anemia
GENETIC TESTING
36. • Better drugs can be obtained by the
knowledge of genetics
• Genetic testing can be used to detect the
mutations regarding genetic disorders like
cystic fibrosis, sickle cell anaemia,
Huntington diseases, etc.
• Tests are also being developed to detect
various cancers
GENETIC TESTING
39. CLONING
Clones are organisms that are exact genetic
copies. Every single bit of their DNA is
identical.
Clones can happen naturally—identical
twins are just one of many examples.
Or they can be made in the lab by:
placing the cell in body of organism
By placing egg cell on the Petri dish to
make embryonic stem cell
40. AGRICULTURAL
DEVELOPMENT
DNA of plant is modified to:
enhance protection of plants against
diseases
Increase the quality and nutritional value
of the plants
Increase the yield of the plants
41. BIOTECHNOLOGY TIMELINE
1996 First mammal cloned from adult
cells
1990s First conviction using genetic
fingerprinting
1996 Development of Affymetrix
GeneChip
1997 First artificial chromosome
42.
43. DISCUSSION
• What is the societal
impression of biotechnology?
• What are the negative
impacts that biotechnology
may have?
44. BACTERIUM
Bacterium is unicellular organism.
Singular – bacterium
Plural - bacteria
Bacteria are prokrayotic – a member of a large
group of unicellular microorganisms which have
cell walls but lack organelles and an organized
nucleus.
There genetic material is a single cell
46. DNA – Deoxyribonucleic Acid
Deoxyribonucleic acid is a molecule that carries the
genetic instructions used in the growth, development,
functioning and reproduction of all known living
organisms and many viruses
Each base pair is attached to a sugar and a phosphate
molecule. Together base, sugar and phosphate are
called nucleotides.
Nucleotides form two strands – double helix
47. DNA REPLICATION
DNA replication is the biological process of
producing two identical replicas of DNA from
one original DNA molecule. This process
occurs in all living organisms and is the basis
for biological inheritance. DNA is made up of
a double helix of two complementary strands.
48. DNA replication. The double helix is unwound
and each strand acts as a template for the next
strand. Bases are matched to synthesize the new
partner strands. DNA replication is the process
of producing two identical replicas from one
original DNA molecule.
49. DNA REPLICATION
There are hydrogen bonds
between two strands.
The first step is to break the
hydrogen bond between bases,
This is done by enzyme called
itelicase .
After unzipping, new bases are
attached to old ones by follwing
the base pairing rule
50. HOW GENES ARE TRANSFERRED
A gene is the unit of heredity and controls
information about the characteristics of an
organism.
Gene transfer is the transfer of gene from one
organism to another i.e. from the donor to the
recipient.
51. GENES TRANSFER
A part of DNA of
donor is cut with the
help of enzyme
A part of DNA of
recipient is cut with
the help of enzyme
52. GENES TRANSFER
New DNA part is transplanted on the
recipient DNA
Enzyme DNA Ligase is used to rejoin
new part of the DNA to recipient