This document discusses gas chromatography. It begins by defining chromatography and describing different chromatography techniques. It then focuses on gas chromatography, explaining that the mobile phase is a carrier gas and the stationary phase is a liquid or polymer coating inside a column. Key components of a gas chromatograph are described, including the carrier gas, injector, column, temperature control, stationary phases, and detectors. The document discusses how gas chromatography can be used for qualitative analysis of compounds and lists some advantages and disadvantages. It concludes by mentioning gas chromatography-mass spectrometry as a modern approach.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
A presentation on column efficiency parameters in chromatography.. A part of gas chromatography in pharmacutical analysis..will be helpful for all mphrm students
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
Gas chromatography is one of the widely used chromatographic techniques, which use inert gas as the mobile phase.
In gas chromatography the components of a sample, after vaporization, are separated by being partitioned between gaseous mobile phase and solid or liquid stationary phase.
The inert gas does not interfere with the analyte but transport the components through the column and facilitate the separation.
The mobile phase is comprised of an inert gas such as helium, argon or nitrogen.
The stationary phase consists of a packed column in which the packing or solid support, or a liquid coat act as stationary phase.
The main principles involved are adsorption and partition for gas solid chromatography and gas liquid chromatography, respectively.
The slides are informative of HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY & its thorough components further its advantages and applications. The comparison of HPLC and HPTLC is explained.
A presentation on column efficiency parameters in chromatography.. A part of gas chromatography in pharmacutical analysis..will be helpful for all mphrm students
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
Gas chromatography is one of the widely used chromatographic techniques, which use inert gas as the mobile phase.
In gas chromatography the components of a sample, after vaporization, are separated by being partitioned between gaseous mobile phase and solid or liquid stationary phase.
The inert gas does not interfere with the analyte but transport the components through the column and facilitate the separation.
The mobile phase is comprised of an inert gas such as helium, argon or nitrogen.
The stationary phase consists of a packed column in which the packing or solid support, or a liquid coat act as stationary phase.
The main principles involved are adsorption and partition for gas solid chromatography and gas liquid chromatography, respectively.
Gas chromatography is widely used techniques for separation of gaseous and volatile substances which are difficult to separate and analyze It is simple and inexpensive method , generally efficient in regard to separation.
Chromatography dates to 1903 in the work of the Russian scientist, Mikhail Tswett. German graduate student Fritz Prior developed solid state gas chromatography in 1947. Archer John Porter Martin, who was awarded the Nobel Prize for his work in developing gas chromatography and he later produced liquid-gas chromatography (1950).
Low amount of sample
Complex mixture.
Gas chromatography is a process of separating component(s) from the given crude drug or mixture by using stationary phase (solid or liquid) and gaseous mobile phase. It involves a sample being vaporized and injected onto the head of the chromatographic
column. The sample is transported through the column by the flow of inert, gaseous
mobile phase. The column itself contains a solid or liquid stationary phase which is adsorbed onto the
surface of an inert solid.
Gas Chromatography in Analytical Analysis.pptxRAHUL PAL
Gas chromatography is a common type of chromatography used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition. Typical uses of GC include testing the purity of a particular substance, or separating the different components of a mixture.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
1.Introduction to Gas chromatography.
2.History of Gas chromatography.
3. Principal of working.
4. Factors affecting gas chromatography.
5. Components of Gas chromatography.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
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Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
2. CONTENTS
• CHROMATOGRAPHY
• DIFFERENT TECHNIQUES OF CHROMATOGRAPHY
• GAS CHROMATOGRAPHY
• REQUIREMENT FOR GAS CHROMATOGRAPHY
• BLOCK DIAGRAM OF GAS CHROMATOGRAPHY
• QUALITATIVE ANALYSIS
• ADVANTAGES AND DISADVANTAGES OF GAS
CHROMATOGRAPHY
• MODERN APPROACH
3. CHROMATOGRAPHY
Laboratory technique for the separation of a mixture.
The mixture is dissolved in a fluid called the mobile phase, which carries it
through a structure holding another material called the stationary phase.
The various constituents of the mixture travel at different speeds, causing
them to separate.
The separation is based on differential partitioning between the mobile and
stationary phases.
Chromatograph: equipment that enables a sophisticated separation, e.g.
gas chromatographic or liquid chromatographic separation.
Chromatogram: Visual output of the chromatograph.
In the case of an optimal separation, different peaks or patterns on the
chromatogram correspond to different components of the separated mixture.
4. TECHNIQUES BASED ON
CHROMATOGRAPHIC BED SHAPE
Column chromatography: Separation technique in which the
stationary bed is within a tube.
Planar chromatography: Separation technique in which the
stationary phase is present as or on a plane.
The plane can be a paper, serving as such or impregnated by a
substance as the stationary bed (paper chromatography) or a layer of
solid particles spread on a support such as a glass plate (thin layer
chromatography)
5. TECHNIQUES BASED ON PHYSICAL STATE
OF MOBILE PHASE
Gas chromatography (GC): Also known as gas-liquid
chromatography, (GLC),
a separation technique in which the mobile phase is a gas. Gas
chromatographic separation is always carried out in a column, which
is typically "packed" or "capillary".
Liquid chromatography (LC): Separation technique in which the
mobile phase is a liquid.
carried out either in a column or a plane
6. GAS CHROMATOGRAPHY
Gas chromatography:
Mobile phase a carrier gas, usually an inert gas such as helium or
an nonreactive gas such as nitrogen.
Hydrogen is preferred for improved separations.
The stationary phase is a microscopic layer of liquid or polymer on an
inert solid support, inside a piece of glass or metal tubing called a column.
The instrument used to perform gas chromatography is called a gas
chromatograph.
The gaseous compounds being analysed interact with the walls of the
column, which is coated with a stationary phase. This causes each
compound to elute at a different time, known as the retention time of the
compound.
The comparison of retention times is what gives GC its analytical
usefulness.
7. CONTINUED….
The function of the stationary phase in the column is to separate different
components, causing each one to exit the column at a different
time(retention time).
As the carrier gas sweeps the analytic molecules through the column, this
motion is inhibited by the adsorption of the analytic molecules either onto
the column walls or onto packing materials in the column.
The rate at which the molecules progress along the column depends on the
strength of adsorption, which in turn depends on the type of molecule and
on the stationary phase materials.
8. CONTINUED……
Since each type of molecule has a different rate of progression, the
various components of the analytic mixture are separated as they
progress along the column and reach the end of the column at different
times(retention Time).
Detector: used to monitor the out let stream from the column; thus,
the time at which each component reaches the outlet and the amount of
that component can be determined.
Generally, substances are identified(qualitatively) by the order in
which they emerge(elute) from the column and by the retention time
of the analytic in the column.
9. REQUIREMENT FOR GAS
CHROMATOGHRAPHY
VOLATILITY:-Tendency of a substance to evaporate at normal
temperatures
POLARITY:-Substance comprised of molecules that contain unbalanced
localized charges (dipoles) is a polar substance.
Polar substances tend to interact with other polar substances and rarely
react to a significant degree with nonpolar substances
When two atoms form a covalent bond, they each share an electron.
Different atoms have different abilities to attract electrons quantified by
their electronegativity values. When one atom in a covalent bond has a
much higher electronegativity than the other, the electron is found closer to
that atom than the weaker one. This creates a dipole effect with a slight
negative charge at the atom the electron favours and a slight positive charge
at the other
12. CARRIER GAS
• he(common),
• others:n2,h2
• safety; non-flammability, cost and efficiency are factors for gas
selection
• inertness
• suitable for the detector
• high purity
• easily available
13. FLOW METER
• Deliver the gas with uniform pressure/flowrate
• Flowmeters:-Rotameter
14. INJECTOR
• Transfers the sample into the column.
• Provides the means to introduce a sample into a continuous flow of
carrier gas.
• Injectors are usually heated to ensure sample’s transfer to a gas
phase.
• Volatile liquid or gaseous sample is injected through a septum.
15. COLUMN
Gas chromatography columns : packed and capillary.
Packed columns :A glass or stainless steel coil(typically1-5m total length and
5mm inner diameter), filled with the stationary phase, or a packing coated with
the stationary phase.
Capillary columns: A thin fused-silica (purified silicate glass) capillary
(typically10-100 m in length and 0.5 mm inner diameter) that has the stationary
phase coated on the inner surface.
Provides much higher separation efficiency than packed columns but are more
easily overloaded by too much sample.
The main chemical attribute regarded when choosing a column is the polarity of
the mixture, The polarity of the sample must closely match the polarity of the
column stationary phase to increase resolution and separation while reducing
runtime.
16. TEMPERATURE CONTROL DEVICES
Temperature Control Devices
Preheaters: convert sample into its vapour form, present along with
injecting devices
Thermostatically controlled oven
Temperature maintenance
in a column is highly essential
for efficient separation.
17. STATIONARY PHASES
For every polar sample, polyethylene glycol(thickess
0.25mircometer) is commonly used as the stationary phase.
Stationary Phases must have:
1.Low volatility
2.Thermal stability
3.Chemical inertness
19. DETECTOR
Placed at the exit of the column.
Employed to detect and provide a quantitative measurement of the
various constituents of the sample
The choice of a particular type of detector is governed by the
following factors:
1. High sensitivity, sufficient enough to provide adequate signal for
even small sample
2. Response should be linear, unaffected by temperature and flowrate.
3. Non distorted shape of peak and non destructive.
4. Detector temperature must not condense the eluted vapours in it.
5. Simple & Inexpensive
6. Applicable to wide range of samples
7. Good reproducibility, rapidity and linearity.
21. QUALITATIVE ANALYSIS
Chromatographic data is presented as a graph of detector response(y-axis)
against retention time(x-axis), which is called a chromatogram.
This provides a spectrum of peaks for a sample representing the analytics
present in a sample eluting from the column at different times.
The number of components in a sample is determined by the number of
peaks.
The amount of a given component in a sample is determined by the area
under the peaks.
The identity of components can be determined by the given retention
times.
22. ADVANTAGES AND DISADVANTAGES
OF GC
ADVANTAGES:-
High Resolution
Very high sensitivity, detect down to 100 ppm.
Very good precision and accuracy.
Very good separation
Time(analysis is short), fast analysis is possible.
Small sample is needed-ml
Good detection system
Quantitatively analysis
DISADVANTAGES :-
Sample must be volatile
Dirty sample choke the capillary
23. MODERN APPROACH
GCMS:-Gas Chromatography Mass Spectrometry
Mass spectrometry: Analytical technique that ionizes chemical
species and sorts the ions based on their mass-to-charge ratio.
Gas chromatography–mass spectrometry (GC-
MS):Analytical method that combines the features of gas-
chromatography and mass spectrometry to identify different
substances within a test sample.
Applications of GC-MS include drug detection, fire investigation,
environmental analysis, explosives investigation, and identification of
unknown samples
25. 2014 IEEE International Conference on Liquid Dielectrics, Bled Slovenia, June 30 -
July 3, 2014
Methanol Detection in Transformer Oils using Gas
Chromatography and Ion Trap Mass Spectrometer
S. Y. Matharagel, Q. Liul, E. Davenportl, G. Wilson2, D. Walker3 and Z.D.
Wang The University of Manchester, Manchester, M13 9PL, UK
2The National Grid Company, Warwick, CV34 6DA, UK
3Scottish Power, Blantyre, G72 OHT, UK
26. Continued…..
• Abstract-Paper ageing is an irreversible process, which has made
paper insulation a lifetime determining factor for transformers.
Chemical indicators in oil such as carbon oxide gases and 2-FAL, are
used to indicate the ageing state of paper as it is difficult to obtain
paper samples to measure DP or tensile strength. Methanol amount in
oil was recently found to be an early-ageing indicator for paper. In this
study, a heads pace gas chromatography mass spectrometry setup was
developed to measure methanol in transformer oil. This setup consists
of an auto sampler with a gas tight syringe, a gas chromatography unit
with 60 m VF-624ms column and a quadrupole ion trap type mass
spectrometer unit. Measurement of several laboratory-aged and
service-aged oil samples were conducted with both external standard
and internal standard calibration methods. Higher methanol values
obtained from internal standard method confirmed that it is more
suitable than external standard method.
27. REFERENCES
A text book of environmental chemistry by “BALRAM PANI”
“https://en.wikipedia.org/wiki/Gas_chromatography%E2%80%93mas
s_spectrometry
https://en.wikipedia.org/wiki/Gas_chromatography
https://www.youtube.com/watch?v=p3_WtEYIhTo
https://www.youtube.com/watch?v=gU2st5-T1Go