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Forage Fermentation:
How to Make Good Silage
Limin Kung, Jr.
Dairy Nutrition & Silage Fermentation Lab
Definition of a high quality
silage/forage
Nutrient recovery
 High nutritive
concentration






(CP, starch, etc.)

High digestibility
by rumen microbes
Harvest Quality and Silo Management Have
Profound Effects on Silage Quality at Feeding
Poor quality forage from field->
Poor harvest and silo management ->
= Poor quality silage at feeding
Poor quality forage at harvest ->
Excellent harvest and silo management ->
= Poor quality silage at feeding
High quality forage at harvest ->
Poor harvest and silo management ->
= Poor quality silage at feeding
High quality forage at harvest ->
Excellent harvest and silo management ->
= Excellent quality silage at feeding
Kung, 2001
Value of Potential Forage Loss
in the Silo –$35/t for Corn Silage
Tons of
Silage/yr

----- DM Losses-----

<10%

15%

20%

1,000

3,500

5,250

7,000

2,000

7,000

10,500

14,000

3,000

10,500

15,750

21,000

Good

Poor Management

Expect losses in milk production and/or greater
concentrate costs when feeding poorer silage
L. Kung, Jr., University of Delaware
Challenges with Alfalfa Silage








High buffering capacity -> slow fermentation
Difficult to wilt quickly
Easily goes clostridial when DM is low (<30%)
Substrate for fermentation can be limiting
during cloudy weather, heavy rains, prolonged
wilts
Difficult to pack when dry
Easily heat damaged at higher DM (>50%)

L. Kung, Jr., University of Delaware
Help with Alfalfa Silage


Minimize wilting time (wide swath?)



Maximize dry down hours during daylight



Wilt to at least 35% DM



Avoid excessive DM (not more than 50%
DM)



Avoid being rained on



Avoid mowing and harvest under cloudy
conditions

L. Kung, Jr., University of Delaware
Consequences of Extreme DM on
Alfalfa and Grass Silages
Too Wet (<30%)

Too Dry (>45-50%)

-Clostridia

-Tough to pack

protein degradation

-Poor stability

DM/energy loss

-Heat damage CP

low digestibility

leading to low

butyric acid/amines

digestibility

-Seepage
L. Kung, Jr., University of Delaware
Consequences of Harvesting Corn
for Silage at Extreme DMs
Too Early

Optimum

Too Late

(<28-30% DM)

(>40% DM)

Low [starch]

Low starch digestion

Low [energy]

32-36% DM

Low acid production

Excess [acid]

Tough to pack

High acetic acid

Poor aerobic stability

Seepage

L. Kung, Jr., University of Delaware
Optimum Harvest Time for
Corn Silage
~32 to 36% whole plant DM
Good starch content and digestibility
Good fiber digestion
Good packing in the silo

L. Kung, Jr., University of Delaware
Effect of Corn Silage Maturity on Starch
Digestion in the Total Tract
adopted from Shaver, 2002
100
100

% Starch 95
Digestion
90
90

Unprocessed

80
80

48

.8

46

45

.6

45

44

43

42

.8

40

.6

39

.4

38

37

.2

35
40
Whole Plant Corn DM%

34

.6

33

.4

32

31

.2

30

36

65

.8

70
70

black layer

.4

½ milk line

.2

75

30

St arch dig. %

85

Dry Mat t er %
*Based on data of Bal et al., 2000; Dhiman et al., 2000; Rojas-Bourrillon et al.1987
Set Chop Length at Harvest!!
Item

C. Silage*

Processed**

Top

3-8%

5-15%

Middle

45-65

>50

Lower

20-30

<30

Pan

<5

<5

*Heinrichs, PSU **Hutjens, UI
Processing for Corn Silage
Cracks the kernel open
to expose starch -> +
digestion
Reduces TMR sorting
Improves silage
packing
L. Kung, Jr., University of Delaware
Fecal Starch and Digestibility

Goal is to have less than 3-4% fecal starch
1%-unit decrease in fecal starch ~ 1 pound more milk
Range in starch: 2.3 – 22.4%

(Ferguson, 2006)
Corn Silage Processing Score
% of starch passing
through a coarse screen
(>4.75 mm)

Processing
Rank

Greater than 70%

Optimum

70% to 50%

Average

Less than 50%

Inadequately
processed

Mertens, 2003
4.75 mm is 1/5 of an inch
Thumb Rules for Assessing the
Degree of Processing
4.75 mm






> 95% of kernels cracked
(70% equal to or smaller
than 1/3 to ¼ kernel size)
Nicking and crushing are
not enough
Cob should be broken to >8
pieces

diameter hole

These pieces would
Not pass through a
4.75 mm hole….
Thus, they are not processed enough!

L. Kung, Jr., University of Delaware
Separate Kernels from Forage in a
Bucket of Water to Assess Kernel Processing

Kung, 2001
Post
Processor?

L. Kung, Jr., University of Delaware
The Goals of Making Silage
Front-end

Back-end

Rapid preservation for maximum
recovery of nutrients
Continued preservation of nutrients
and excellent “shelf life”
Microbes at work in silage – silage
making is like a war – good bugs must win!


“Good bugs”


Lactic acid bacteria
heterofermentative
 homofermentative




“Bad bugs”
Yeasts
 Molds
 Clostridia
 Enterobacteria


Kung, 2001
Conditions
Front end fermentation

No Air
sugars

Back end storage

No Air
Stable, high quality

lactic acid

acetic acid
pH
> 45 C
35 C

Days of Ensiling

Kung, 2001
However…


A good front end fermentation does not
automatically lead to stability during storage
and feed out (backend)



A high concentration of lactic acid and/or low
pH alone does not automatically equate to a
stabile if silage is exposed to air
Ideal Fermentation but Poor Storage Conditions
Front end fermentation Back end storage
No air

Exposure to Air

sugars
lactic acid

acetic acid
pH
>120 F

Days of Ensiling

Kung, 2001
105 F
The “Domino Effect” From Air
on Aerobic Spoilage – Bad Bugs
Silage is exposed to air
Yeasts ‘wake up’ and degrade lactic acid
Numbers of yeasts increase
Highly degradable nutrients are destroyed
Heat is produced
pH increases
Molds/bacteria ‘wake up’ causing
further spoilage
More heating
Massive spoilage
L. Kung, Jr., University of Delaware
The Negative Relationship Between
Number of Yeasts and Aerobic Stability
200

Hours
Of 160
Stability
Before
120
Spoilage

o o
o
o

80

o

o

o

o
o

o

40
0
1000
Kung, University of Delaware

o

o
o
10000

100000

Yeast, cfu/g

o
o
1000000
Changes in DMI When Heifers are Fed
Aerobically Spoiling Feed

Der Bedrosian et al., 2012

a

b

107,151 yeast/g
Undesirable Clostridial Fermentation
Front end fermentation

Back end storage
Poor quality forage
High NH3 and butyric acid

Forage too wet
Low sugars
Clostridia
pH
sugars
acetic acid
lactic acid

>120 F
105 F

Days of Ensiling

butyric acid
Silo Filling
•Fill quickly
•Pack tightly
•14-16 lb DM/ft3 CS
(225-260 kg DM/m3)
•15-17 lb DM/ft3 AS
(240-270 kg DM/m3)
•6-8 inch (15-20 cm)
layers
•Heavy tractors
Plastic on the Sidewalls

Greenfield, 2003
Cover With Plastic and Tires
Immediately







White plastic better than
black
6 is better than 4 mil
Small bunks – consider 2
layers? (thin layer on
bottom is best)
More weight on
seams/edges

L. Kung, Jr., University of Delaware
Oxygen Barrier Plastics

Kung and McDonell, 2005
No Chia-Pet Covers!!!

Kung, 2007
Check the teeth on your baggers
Sharp teeth =
-faster and tighter bagging
-cleaner cuts on forage (less juicing)

L. Kung, Jr., University of Delaware
Keep Plastic Down at the
Feeding Face

Kung, 2006

Kung, 2006
Face Management
- Remove a minimum ??? inch/d
- Remove more in hot weather and for
drier/poorly packed silages
- Keep face clean, minimize face damage
- Knock down only enough silage to feed
“The Management Disconnect”
This farm was so happy that they were using a face shaver!

Face Shavers
 (+) Most useful when density
is low and/or feed rate is slow
 May save 3% in DM
 Savings in animal
performance?
 (-) Longer feeding time
L. Kung, Jr., University of Delaware
Microbial Inoculants Can Improve Silage
Fermentation and Aerobic Stability


Help at the Front – Homolactic acid bacteria






Help at the Back – Lactobacillus buchneri






Faster fermentation
Reduce clostridia
Improved DM/energy recovery
Improved aerobic stability
Fresher feed
Less spoiling

Dual purpose – Combination of the above
Approaches For Using Silage Inoculants –
Tools For Maintaining Good Fermentations

1. Preventing a clostridial fermentation
- wet alfalfa and grasses
- quick attainment of a low pH
- conventional homolactic acid bacteria

2. Improving aerobic stability
- HMC and corn silages
- L. buchneri products

3. Making a good fermentation better
- improvements in DM recovery
Modified from Muck 2008
General Inoculant
Recommendations


By crop






Alfalfa and grasss -> Ho* (>40% DM -> LbC**)
Corn silage -> Ho or LbC (if aerobic challenges)
HMC -> LbC

By DM



< 30% DM -> Ho (avoid LB)
> 40% DM -> LbC

*Ho = homolactic acid bacteria based additive
**LbC = L. buchneri + homolactic acid bacteria
Managing Your Inoculants


Calibrate applicators frequently



Optimize distribution



Liquid applied is better in drier (>40% DM)
forages



Do not mix inoculants with hot water



Keep water temp in tanks < 95-100 F
Numbers of Viable Bacteria Were Lower in
Tanks with High Water Temperatures
r2 = 0.39
P < 0.01

16
Windle and Kung, University of Delaware, 2013

27

38

49
Summary








Forage quality and silo management have
profound effects on net farm income
Harvest forage crops at optimum maturity
Follow best management practices for
processing and storage
Use tools available to measure standards
Use inoculants best suited for your needs
THANK YOU!!!!

LKSILAGE@UDEL.EDU
http://ag.udel.edu/anfs/faculty/Kung.html

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Forage Fermentation: How to Make Good Silage

  • 1. Forage Fermentation: How to Make Good Silage Limin Kung, Jr. Dairy Nutrition & Silage Fermentation Lab
  • 2. Definition of a high quality silage/forage Nutrient recovery  High nutritive concentration    (CP, starch, etc.) High digestibility by rumen microbes
  • 3. Harvest Quality and Silo Management Have Profound Effects on Silage Quality at Feeding Poor quality forage from field-> Poor harvest and silo management -> = Poor quality silage at feeding Poor quality forage at harvest -> Excellent harvest and silo management -> = Poor quality silage at feeding High quality forage at harvest -> Poor harvest and silo management -> = Poor quality silage at feeding High quality forage at harvest -> Excellent harvest and silo management -> = Excellent quality silage at feeding Kung, 2001
  • 4. Value of Potential Forage Loss in the Silo –$35/t for Corn Silage Tons of Silage/yr ----- DM Losses----- <10% 15% 20% 1,000 3,500 5,250 7,000 2,000 7,000 10,500 14,000 3,000 10,500 15,750 21,000 Good Poor Management Expect losses in milk production and/or greater concentrate costs when feeding poorer silage L. Kung, Jr., University of Delaware
  • 5. Challenges with Alfalfa Silage       High buffering capacity -> slow fermentation Difficult to wilt quickly Easily goes clostridial when DM is low (<30%) Substrate for fermentation can be limiting during cloudy weather, heavy rains, prolonged wilts Difficult to pack when dry Easily heat damaged at higher DM (>50%) L. Kung, Jr., University of Delaware
  • 6. Help with Alfalfa Silage  Minimize wilting time (wide swath?)  Maximize dry down hours during daylight  Wilt to at least 35% DM  Avoid excessive DM (not more than 50% DM)  Avoid being rained on  Avoid mowing and harvest under cloudy conditions L. Kung, Jr., University of Delaware
  • 7. Consequences of Extreme DM on Alfalfa and Grass Silages Too Wet (<30%) Too Dry (>45-50%) -Clostridia -Tough to pack protein degradation -Poor stability DM/energy loss -Heat damage CP low digestibility leading to low butyric acid/amines digestibility -Seepage L. Kung, Jr., University of Delaware
  • 8. Consequences of Harvesting Corn for Silage at Extreme DMs Too Early Optimum Too Late (<28-30% DM) (>40% DM) Low [starch] Low starch digestion Low [energy] 32-36% DM Low acid production Excess [acid] Tough to pack High acetic acid Poor aerobic stability Seepage L. Kung, Jr., University of Delaware
  • 9. Optimum Harvest Time for Corn Silage ~32 to 36% whole plant DM Good starch content and digestibility Good fiber digestion Good packing in the silo L. Kung, Jr., University of Delaware
  • 10. Effect of Corn Silage Maturity on Starch Digestion in the Total Tract adopted from Shaver, 2002 100 100 % Starch 95 Digestion 90 90 Unprocessed 80 80 48 .8 46 45 .6 45 44 43 42 .8 40 .6 39 .4 38 37 .2 35 40 Whole Plant Corn DM% 34 .6 33 .4 32 31 .2 30 36 65 .8 70 70 black layer .4 ½ milk line .2 75 30 St arch dig. % 85 Dry Mat t er % *Based on data of Bal et al., 2000; Dhiman et al., 2000; Rojas-Bourrillon et al.1987
  • 11. Set Chop Length at Harvest!! Item C. Silage* Processed** Top 3-8% 5-15% Middle 45-65 >50 Lower 20-30 <30 Pan <5 <5 *Heinrichs, PSU **Hutjens, UI
  • 12. Processing for Corn Silage Cracks the kernel open to expose starch -> + digestion Reduces TMR sorting Improves silage packing L. Kung, Jr., University of Delaware
  • 13. Fecal Starch and Digestibility Goal is to have less than 3-4% fecal starch 1%-unit decrease in fecal starch ~ 1 pound more milk Range in starch: 2.3 – 22.4% (Ferguson, 2006)
  • 14. Corn Silage Processing Score % of starch passing through a coarse screen (>4.75 mm) Processing Rank Greater than 70% Optimum 70% to 50% Average Less than 50% Inadequately processed Mertens, 2003
  • 15. 4.75 mm is 1/5 of an inch
  • 16. Thumb Rules for Assessing the Degree of Processing 4.75 mm    > 95% of kernels cracked (70% equal to or smaller than 1/3 to ¼ kernel size) Nicking and crushing are not enough Cob should be broken to >8 pieces diameter hole These pieces would Not pass through a 4.75 mm hole…. Thus, they are not processed enough! L. Kung, Jr., University of Delaware
  • 17. Separate Kernels from Forage in a Bucket of Water to Assess Kernel Processing Kung, 2001
  • 18. Post Processor? L. Kung, Jr., University of Delaware
  • 19. The Goals of Making Silage Front-end Back-end Rapid preservation for maximum recovery of nutrients Continued preservation of nutrients and excellent “shelf life”
  • 20. Microbes at work in silage – silage making is like a war – good bugs must win!  “Good bugs”  Lactic acid bacteria heterofermentative  homofermentative   “Bad bugs” Yeasts  Molds  Clostridia  Enterobacteria  Kung, 2001
  • 21. Conditions Front end fermentation No Air sugars Back end storage No Air Stable, high quality lactic acid acetic acid pH > 45 C 35 C Days of Ensiling Kung, 2001
  • 22. However…  A good front end fermentation does not automatically lead to stability during storage and feed out (backend)  A high concentration of lactic acid and/or low pH alone does not automatically equate to a stabile if silage is exposed to air
  • 23. Ideal Fermentation but Poor Storage Conditions Front end fermentation Back end storage No air Exposure to Air sugars lactic acid acetic acid pH >120 F Days of Ensiling Kung, 2001 105 F
  • 24. The “Domino Effect” From Air on Aerobic Spoilage – Bad Bugs Silage is exposed to air Yeasts ‘wake up’ and degrade lactic acid Numbers of yeasts increase Highly degradable nutrients are destroyed Heat is produced pH increases Molds/bacteria ‘wake up’ causing further spoilage More heating Massive spoilage L. Kung, Jr., University of Delaware
  • 25. The Negative Relationship Between Number of Yeasts and Aerobic Stability 200 Hours Of 160 Stability Before 120 Spoilage o o o o 80 o o o o o o 40 0 1000 Kung, University of Delaware o o o 10000 100000 Yeast, cfu/g o o 1000000
  • 26. Changes in DMI When Heifers are Fed Aerobically Spoiling Feed Der Bedrosian et al., 2012 a b 107,151 yeast/g
  • 27. Undesirable Clostridial Fermentation Front end fermentation Back end storage Poor quality forage High NH3 and butyric acid Forage too wet Low sugars Clostridia pH sugars acetic acid lactic acid >120 F 105 F Days of Ensiling butyric acid
  • 28. Silo Filling •Fill quickly •Pack tightly •14-16 lb DM/ft3 CS (225-260 kg DM/m3) •15-17 lb DM/ft3 AS (240-270 kg DM/m3) •6-8 inch (15-20 cm) layers •Heavy tractors
  • 29. Plastic on the Sidewalls Greenfield, 2003
  • 30. Cover With Plastic and Tires Immediately     White plastic better than black 6 is better than 4 mil Small bunks – consider 2 layers? (thin layer on bottom is best) More weight on seams/edges L. Kung, Jr., University of Delaware
  • 31. Oxygen Barrier Plastics Kung and McDonell, 2005
  • 33. Check the teeth on your baggers Sharp teeth = -faster and tighter bagging -cleaner cuts on forage (less juicing) L. Kung, Jr., University of Delaware
  • 34. Keep Plastic Down at the Feeding Face Kung, 2006 Kung, 2006
  • 35. Face Management - Remove a minimum ??? inch/d - Remove more in hot weather and for drier/poorly packed silages - Keep face clean, minimize face damage - Knock down only enough silage to feed
  • 36. “The Management Disconnect” This farm was so happy that they were using a face shaver! Face Shavers  (+) Most useful when density is low and/or feed rate is slow  May save 3% in DM  Savings in animal performance?  (-) Longer feeding time L. Kung, Jr., University of Delaware
  • 37. Microbial Inoculants Can Improve Silage Fermentation and Aerobic Stability  Help at the Front – Homolactic acid bacteria     Help at the Back – Lactobacillus buchneri     Faster fermentation Reduce clostridia Improved DM/energy recovery Improved aerobic stability Fresher feed Less spoiling Dual purpose – Combination of the above
  • 38. Approaches For Using Silage Inoculants – Tools For Maintaining Good Fermentations 1. Preventing a clostridial fermentation - wet alfalfa and grasses - quick attainment of a low pH - conventional homolactic acid bacteria 2. Improving aerobic stability - HMC and corn silages - L. buchneri products 3. Making a good fermentation better - improvements in DM recovery Modified from Muck 2008
  • 39. General Inoculant Recommendations  By crop     Alfalfa and grasss -> Ho* (>40% DM -> LbC**) Corn silage -> Ho or LbC (if aerobic challenges) HMC -> LbC By DM   < 30% DM -> Ho (avoid LB) > 40% DM -> LbC *Ho = homolactic acid bacteria based additive **LbC = L. buchneri + homolactic acid bacteria
  • 40. Managing Your Inoculants  Calibrate applicators frequently  Optimize distribution  Liquid applied is better in drier (>40% DM) forages  Do not mix inoculants with hot water  Keep water temp in tanks < 95-100 F
  • 41. Numbers of Viable Bacteria Were Lower in Tanks with High Water Temperatures r2 = 0.39 P < 0.01 16 Windle and Kung, University of Delaware, 2013 27 38 49
  • 42. Summary      Forage quality and silo management have profound effects on net farm income Harvest forage crops at optimum maturity Follow best management practices for processing and storage Use tools available to measure standards Use inoculants best suited for your needs

Editor's Notes

  1. Der Bedrosian et al 2012 showed that feeding spoiled TMR to heifers significantly decreased DMI by about 1kg. There was also a larger difference in the number of yeast found in spoiled vs unspoiled TMR. Unspoiled TRM contained over 100,000 yeast per gram while spoiled TMR had over 66 million yeast per gram. This increase in number of yeast may have contributed to the decrease in DMI.
  2. These are the results of the effect of tank temperature (point) on bacterial counts (point) Bacteria died as temperatures increased More than 10% of the tanks had temperatures higher than 100°F The decrease was much more dramatic at temperatures above 95°F DE 1000 and 1010 seemed to keep the inoculants cooler