Liquid-liquid extraction is a technique used to separate compounds based on their relative solubilities in two different immiscible liquids. The key steps are: 1) weighing and preparing samples, 2) adding an organic solvent to extract the compound of interest, 3) mixing the layers and allowing them to separate, and 4) transferring and collecting the extracted layers. Proper labeling and disposal of waste is important for safety.

1. Liquid-Liquid Extraction
techniques

2. Use balance to
weigh solids

3. TARE by pressing
->0/T<- Test tube is supported in a beaker
-> to subtract the weight
of the glassware

4. Add chemical to glassware after removing it
from the balance

5. Record measurement

6. Add organic solvent

8. Mix with pipette,
aspirate and expel

9. Solid has dissolved
in organic solvent

10. Add aqueous layer

11. meniscus

12. - Use pipette to mix layers
- Allow mixture to separate

13. Layers are separated

14. Label receiving test tube

15. Pipette
Both test tubes are held with
the same hand
transfer bottom layer
transferred
aqueous layer

16. Precipitate solid from aqueous layer

17. solid formation

18. Label receiving vessel

19. ether layer
compressed air jet
carefully turn on
compressed air to
get a gentle stream

20. solid deposits on the
side of the test tube
ether evaporates

21. Clean up

22. solids that are insoluble in water (benzophenone) are rinsed with
acetone into the flammable waste

23. Discard used chemicals in the
appropriate waste collector

24. Rinse all glassware with hot
water

25. Place rinsed glassware in proper basket