This document discusses Salmonella and Shigella, which are foodborne pathogens that can cause diseases like typhoid fever and dysentery in humans. It provides details on their transmission, pathogenesis, clinical symptoms, treatment, and methods for isolation and enumeration from samples. Salmonella is usually transmitted through contaminated foods of animal origin and can cause diseases ranging from gastroenteritis to typhoid fever. Shigella transmission occurs through the fecal-oral route and mainly affects children, causing shigellosis. Diagnosis involves culturing samples in selective media. Treatment requires antibiotics while prevention relies on sanitation and hygiene practices.
The document discusses Bacillus anthracis, the bacterium that causes anthrax. It describes the morphological and biochemical characteristics of B. anthracis, how it causes disease, methods for laboratory diagnosis of anthrax, treatment and post-exposure prophylaxis. It also discusses anthrax as a potential biological warfare agent and Pakistan's experience investigating suspected anthrax cases after 2001.
E coli, klebsiella, enterobacter lecture notesBruno Mmassy
E. coli is a common Gram-negative bacteria that normally inhabits the gastrointestinal tract. Certain strains can cause illnesses such as gastroenteritis, urinary tract infections, and neonatal meningitis. E. coli is biochemically identified using tests such as IMViC, which examines indole production, methyl red, Voges-Proskauer, and citrate utilization. Treatment involves antibiotics such as ampicillin, but there is increasing antibiotic resistance in many strains. Preventing the spread of pathogenic E. coli requires proper sanitation and food handling.
This document provides information on Enterobacteriaceae, a family of Gram-negative bacteria commonly found in the intestines. It describes their characteristics, including being non-spore forming rods that ferment glucose and reduce nitrates. Major genera discussed include Escherichia, Salmonella, Shigella, Klebsiella, and Proteus. E. coli is examined in more depth, outlining its morphology, culture characteristics, biochemical reactions, antigens, and virulence factors such as toxins. Pathogenic E. coli types are classified and their diseases and toxins described, including enterotoxigenic, enteropathogenic, enterohemorrhagic, and enteroinvasive E. coli.
This document provides information on the genus Escherichia coli. It discusses the morphology, culture characteristics, biochemical reactions, antigenic structure, and virulence factors of E. coli. Key points include:
- E. coli is a gram-negative, facultative anaerobic rod that is a normal inhabitant of the gastrointestinal tract.
- It ferments glucose with acid and gas production and is capable of reducing nitrates to nitrites.
- E. coli has O, H, and K surface antigens that are used for serotyping. The O antigen lipopolysaccharide contributes to virulence.
- Virulence factors include surface antigens, fimbriae, and toxins
The document provides information about the genus Vibrio, including Vibrio cholerae which causes cholera. It discusses the morphology, growth characteristics, pathogenic species, and pathogenesis of Vibrio. Vibrio are common bacteria found in surface waters worldwide. Vibrio cholerae serogroups O1 and O139 cause cholera in humans through an enterotoxin that causes severe diarrhea and dehydration. The document details the identification and isolation of Vibrio species using different cultural techniques.
- Escherichia coli (E. coli) is a common bacteria that normally lives in the intestines of humans and animals. However, some strains of E. coli can cause illness, including diarrhea.
- E. coli that causes diarrhea is divided into six groups that produce diarrhea through different pathogenic mechanisms, such as the production of heat-stable or heat-labile toxins. One group, enterotoxigenic E. coli (ETEC), commonly causes travelers' diarrhea.
- In addition to diarrhea, E. coli can cause other illnesses like urinary tract infections, wound or blood infections, and even sepsis. The type of specimen collected for testing depends on the suspected infection.
Isolation and identification of salmonella &e.coliNoman Ch
This presentation is made by concerning three books. The data used in this is mainly revolve about poultry point of view.
REFERENCE
Isolation and identification of avian pathogen(AAAP)
The document discusses Bacillus anthracis, the bacterium that causes anthrax. It describes the morphological and biochemical characteristics of B. anthracis, how it causes disease, methods for laboratory diagnosis of anthrax, treatment and post-exposure prophylaxis. It also discusses anthrax as a potential biological warfare agent and Pakistan's experience investigating suspected anthrax cases after 2001.
E coli, klebsiella, enterobacter lecture notesBruno Mmassy
E. coli is a common Gram-negative bacteria that normally inhabits the gastrointestinal tract. Certain strains can cause illnesses such as gastroenteritis, urinary tract infections, and neonatal meningitis. E. coli is biochemically identified using tests such as IMViC, which examines indole production, methyl red, Voges-Proskauer, and citrate utilization. Treatment involves antibiotics such as ampicillin, but there is increasing antibiotic resistance in many strains. Preventing the spread of pathogenic E. coli requires proper sanitation and food handling.
This document provides information on Enterobacteriaceae, a family of Gram-negative bacteria commonly found in the intestines. It describes their characteristics, including being non-spore forming rods that ferment glucose and reduce nitrates. Major genera discussed include Escherichia, Salmonella, Shigella, Klebsiella, and Proteus. E. coli is examined in more depth, outlining its morphology, culture characteristics, biochemical reactions, antigens, and virulence factors such as toxins. Pathogenic E. coli types are classified and their diseases and toxins described, including enterotoxigenic, enteropathogenic, enterohemorrhagic, and enteroinvasive E. coli.
This document provides information on the genus Escherichia coli. It discusses the morphology, culture characteristics, biochemical reactions, antigenic structure, and virulence factors of E. coli. Key points include:
- E. coli is a gram-negative, facultative anaerobic rod that is a normal inhabitant of the gastrointestinal tract.
- It ferments glucose with acid and gas production and is capable of reducing nitrates to nitrites.
- E. coli has O, H, and K surface antigens that are used for serotyping. The O antigen lipopolysaccharide contributes to virulence.
- Virulence factors include surface antigens, fimbriae, and toxins
The document provides information about the genus Vibrio, including Vibrio cholerae which causes cholera. It discusses the morphology, growth characteristics, pathogenic species, and pathogenesis of Vibrio. Vibrio are common bacteria found in surface waters worldwide. Vibrio cholerae serogroups O1 and O139 cause cholera in humans through an enterotoxin that causes severe diarrhea and dehydration. The document details the identification and isolation of Vibrio species using different cultural techniques.
- Escherichia coli (E. coli) is a common bacteria that normally lives in the intestines of humans and animals. However, some strains of E. coli can cause illness, including diarrhea.
- E. coli that causes diarrhea is divided into six groups that produce diarrhea through different pathogenic mechanisms, such as the production of heat-stable or heat-labile toxins. One group, enterotoxigenic E. coli (ETEC), commonly causes travelers' diarrhea.
- In addition to diarrhea, E. coli can cause other illnesses like urinary tract infections, wound or blood infections, and even sepsis. The type of specimen collected for testing depends on the suspected infection.
Isolation and identification of salmonella &e.coliNoman Ch
This presentation is made by concerning three books. The data used in this is mainly revolve about poultry point of view.
REFERENCE
Isolation and identification of avian pathogen(AAAP)
Salmonella is a genus of bacteria that can cause infections in humans and other animals. There are over 2000 serotypes of Salmonella, with some causing typhoid fever and paratyphoid fever through consumption of contaminated food or water. S. Typhi specifically causes typhoid fever, a systemic illness involving the gastrointestinal tract and bloodstream. The disease progresses as the bacteria penetrate the intestines, spread to internal organs, and multiply before reentering the bloodstream and causing symptoms like sustained fever and abdominal pain. Carriers of S. Typhi pose a public health risk by potentially spreading the infection through food preparation.
- Listeria was first isolated in 1926 from infected rabbits and named Bacterium monocytogenes. It was later renamed Listeria in 1940.
- Listeria monocytogenes is a pathogenic bacteria that can cause listeriosis in humans and animals through the consumption of contaminated food. It is particularly associated with consumption of contaminated silage by cattle and sheep.
- Symptoms of listeriosis in animals include dullness, circling behavior, head tilt, facial paralysis, and in some cases abortion. It primarily infects the central nervous system.
E. coli is a common bacteria that lives in the intestines of humans and animals. While most strains are harmless, some can cause illness. Symptoms of infection include abdominal cramps, diarrhea, nausea and vomiting. The bacteria is rod-shaped and uses various virulence factors like fimbriae, toxins and flagella to cause disease. Antibiotics are used to treat infections and vaccines may help reduce human illnesses, but are not widely used due to costs.
- Vibrio are Gram-negative, curved rods or comma-shaped bacteria that are highly motile due to single polar flagella. The most important member is Vibrio cholerae, which causes the disease cholera.
- V. cholerae was first isolated from cholera patients in Egypt in 1883. It produces a heat-labile enterotoxin that increases intracellular cAMP, causing a massive loss of water and electrolytes from the intestines.
- Other medically important Vibrio species include V. paraheamolyticus and V. vulnificus, which are halophilic and can cause food poisoning or wound infections associated with marine environments.
Campylobacter is a common cause of foodborne illness worldwide and Campylobacter jejuni is a typical organism, which is a gram-negative, motile bacterium that causes both intestinal and systemic infections. Key virulence factors of Campylobacter include lipopolysaccharides and enterotoxins, and pathogenesis involves oral transmission from contaminated food or animals followed by colonization of the small intestine. Symptoms include acute diarrhea and abdominal pain that are usually self-limiting but antibiotics may be used for severe or prolonged cases.
The document provides an overview of the Enterobacteriaceae family of Gram-negative bacteria. It discusses key characteristics such as being facultative anaerobes that ferment carbohydrates and possess complex antigenic structures. Major pathogens within Enterobacteriaceae like Escherichia coli, Salmonella, and Shigella are then described in more detail, outlining their classification, virulence factors, clinical diseases caused, and methods of transmission and prevention.
Shigella is a genus of bacteria that can cause shigellosis (dysentery). It was first isolated in 1896 in Japan by Kiyoshi Shiga. There are four subgroups of Shigella - S. dysenteriae, S. flexneri, S. boydii, and S. sonnei - classified based on biochemical and antigenic characteristics. Shigella is transmitted via the fecal-oral route and causes symptoms ranging from asymptomatic infection to severe dysentery. It produces Shiga toxin which is cytotoxic and responsible for the bloody diarrhea and tenesmus associated with shigellosis. Diagnosis involves culturing fresh stool samples on selective media like deoxycholate cit
Medical Microbiology Laboratory (Enterobacteriaceae - V)Hussein Al-tameemi
The document discusses the Enterobacteriaceae family of bacteria, including Yersinia and Morganella species. It notes that Enterobacteriaceae are gram-negative, facultatively anaerobic bacilli that are commonly found in the intestines of humans and animals. It provides classifications based on lactose fermentation and describes various media used to differentiate types. Specific details are given on Yersinia enterocolitica, Y. pseudotuberculosis, and Y. pestis. Information is also provided on the taxonomy, characteristics, infections, diagnosis and culture of Morganella morganii.
1. Enterobacteriaceae are a family of Gram-negative bacteria that are commonly found in the intestines of humans and animals. They include several important pathogens like Escherichia coli, Salmonella, Shigella, Klebsiella, and Enterobacter.
2. Members of Enterobacteriaceae are facultative anaerobes and most have peritrichous flagella. Some important genera include Escherichia, Salmonella, Shigella, Klebsiella, Citrobacter, Enterobacter, Proteus, Serratia, and Morganella.
3. Several species of Enterobacteriaceae can cause diseases like diarrhea, intestinal infections, urinary tract infections
This document discusses Enterobacteriaceae, a family of Gram-negative bacteria that includes many common pathogens. It provides details on their characteristics, identification, classification based on lactose fermentation, and important genera such as Escherichia coli. Reasons why E. coli is commonly used for gene cloning are described, including its genetic simplicity, rapid growth, safety, extensive prior study, and ability to host foreign DNA. Identification of Enterobacteriaceae involves examining biochemical reactions and growth on selective media like MacConkey agar.
Staphylococcus is a genus of gram-positive cocci that commonly causes infections in humans. Staphylococcus aureus is one of the most important pathogenic species, able to produce several toxins and enzymes that contribute to its virulence. These include hemolysins, leukocidins, enterotoxins, toxic shock syndrome toxin, and exfoliative toxins. S. aureus can cause a variety of infections through cutaneous, respiratory, or deep tissue routes. Laboratory identification involves gram stain morphology, culture characteristics such as catalase and coagulase production, and biochemical testing. Methicillin-resistant S. aureus is an important antibiotic resistant variant.
This document discusses Escherichia coli (E. coli), a common bacterium found in the intestines of humans and animals. It describes E. coli's morphology, identification, and ability to cause diseases like urinary tract infections, neonatal meningitis, and intestinal diseases. Prevention methods are outlined, including good hygiene practices like handwashing and thoroughly cooking meats to avoid transmission. Treatment focuses on fluid replacement rather than antibiotics in most cases unless the disease has progressed to a systemic stage.
Diagnosis of fungal disease by Dr. Manoj karkimanojj123
Early diagnosis of fungal infection is critical for effective treatment. History, clinical signs, gross pathology and in few cases intradermal skin test are all of the value in the diagnosis of clinical specimens.
This document provides information about Gram positive bacilli (rods), specifically the genus Clostridium. It discusses the characteristics of Clostridium including that they are spore-forming, anaerobic, Gram-positive rods. It also describes several medically important Clostridium species (C. tetani, C. botulinum, C. perfringens, C. difficile), their samples and microscopic features, culture characteristics, biochemical tests for identification, and a simple differentiation chart comparing characteristics. The document is authored by Hussein A. Abid, a medical laboratory scientist and chairman of the Iraqi Medical Laboratory Association.
This document discusses Salmonella, including its isolation, morphology, cultural characteristics, biochemical activities, epidemiology, virulence factors, pathogenesis, clinical diseases, laboratory diagnosis, and treatment. Salmonella was first isolated in 1885 and causes diseases like typhoid fever, paratyphoid fever, and foodborne illness in humans. It is a gram-negative rod found in the intestines of animals and can contaminate foods. Diagnosis involves culturing specimens from blood, feces, urine or vomit. Treatment includes antibiotics like chloramphenicol, ampicillin, and ciprofloxacin.
Shigella dysenteriae is a gram-negative, non-motile bacillus that causes dysentery. It grows well at 37°C in nutrient broth, forming colorless colonies on MacConkey agar and red colonies without black centers on XLD agar. S. dysenteriae infection is caused by ingesting contaminated food or water and leads to dysentery characterized by bloody mucus in stool. Laboratory diagnosis involves examining stool samples microscopically for pus cells and macrophages, culturing on selective media to isolate non-lactose fermenting colonies, and conducting biochemical tests and slide agglutination with specific antisera. Tetracycline and chloramphenicol are used
This document provides information on the bacteria Neisseria, including its classification, properties, species of medical importance (N. gonorrhoeae and N. meningitidis), laboratory diagnosis, treatment, and prevention. It discusses the pathogenesis, virulence factors, and diagnosis of N. meningitidis which causes meningitis, and N. gonorrhoeae which causes gonorrhea. Methods for isolating and identifying these bacteria in the laboratory are also summarized.
Bacillus is a genus of rod-shaped, Gram-positive bacteria that can form dormant endospores. The document focuses on Bacillus anthracis, which causes anthrax. It describes the morphology, cultural characteristics, virulence factors, and methods of diagnosis and prevention of B. anthracis. Key points include that B. anthracis forms encapsulated, non-motile rods and terminal spores. The anthrax toxins are composed of lethal factor, edema factor, and protective antigen, which combine to cause disease. Diagnosis involves microscopy, culture, and serology. Prevention for humans involves vaccination with anthrax toxoid and occupational hygiene, while animals are vaccinated with attenuated spore
The document discusses various bacterial infections that can cause diarrhea, including salmonellosis, typhoid fever, paratyphoid fever, non-typhoidal salmonella infections, shigellosis, cholera, and escherichiosis. It describes the causative organisms, pathogenesis, clinical presentation, diagnosis, and treatment of each infection. Key points include that salmonella can cause gastroenteritis, typhoid fever is caused by S. typhi, paratyphoid fever is milder illness caused by S. paratyphi, shigella causes dysentery, V. cholerae toxin causes cholera's profuse diarrhea, and E. coli strains like ETEC, EPE
This document provides an overview of Salmonella, including Salmonella enterica and Salmonella bongori. It discusses Salmonella serotyping based on surface structures. The pathogenesis and immunity of Salmonella is described, noting how it attaches and invades the intestines. Two pathogenicity islands regulate these processes. Epidemiology sections explain the animal reservoirs and most common sources of human infections like poultry, eggs and dairy. Clinical diseases caused include gastroenteritis, septicemia, enteric fever and asymptomatic colonization. Laboratory diagnosis focuses on culturing Salmonella from blood, feces or bone marrow. Biochemical tests are used to identify isolates.
Salmonella is a genus of bacteria that can cause infections in humans and other animals. There are over 2000 serotypes of Salmonella, with some causing typhoid fever and paratyphoid fever through consumption of contaminated food or water. S. Typhi specifically causes typhoid fever, a systemic illness involving the gastrointestinal tract and bloodstream. The disease progresses as the bacteria penetrate the intestines, spread to internal organs, and multiply before reentering the bloodstream and causing symptoms like sustained fever and abdominal pain. Carriers of S. Typhi pose a public health risk by potentially spreading the infection through food preparation.
- Listeria was first isolated in 1926 from infected rabbits and named Bacterium monocytogenes. It was later renamed Listeria in 1940.
- Listeria monocytogenes is a pathogenic bacteria that can cause listeriosis in humans and animals through the consumption of contaminated food. It is particularly associated with consumption of contaminated silage by cattle and sheep.
- Symptoms of listeriosis in animals include dullness, circling behavior, head tilt, facial paralysis, and in some cases abortion. It primarily infects the central nervous system.
E. coli is a common bacteria that lives in the intestines of humans and animals. While most strains are harmless, some can cause illness. Symptoms of infection include abdominal cramps, diarrhea, nausea and vomiting. The bacteria is rod-shaped and uses various virulence factors like fimbriae, toxins and flagella to cause disease. Antibiotics are used to treat infections and vaccines may help reduce human illnesses, but are not widely used due to costs.
- Vibrio are Gram-negative, curved rods or comma-shaped bacteria that are highly motile due to single polar flagella. The most important member is Vibrio cholerae, which causes the disease cholera.
- V. cholerae was first isolated from cholera patients in Egypt in 1883. It produces a heat-labile enterotoxin that increases intracellular cAMP, causing a massive loss of water and electrolytes from the intestines.
- Other medically important Vibrio species include V. paraheamolyticus and V. vulnificus, which are halophilic and can cause food poisoning or wound infections associated with marine environments.
Campylobacter is a common cause of foodborne illness worldwide and Campylobacter jejuni is a typical organism, which is a gram-negative, motile bacterium that causes both intestinal and systemic infections. Key virulence factors of Campylobacter include lipopolysaccharides and enterotoxins, and pathogenesis involves oral transmission from contaminated food or animals followed by colonization of the small intestine. Symptoms include acute diarrhea and abdominal pain that are usually self-limiting but antibiotics may be used for severe or prolonged cases.
The document provides an overview of the Enterobacteriaceae family of Gram-negative bacteria. It discusses key characteristics such as being facultative anaerobes that ferment carbohydrates and possess complex antigenic structures. Major pathogens within Enterobacteriaceae like Escherichia coli, Salmonella, and Shigella are then described in more detail, outlining their classification, virulence factors, clinical diseases caused, and methods of transmission and prevention.
Shigella is a genus of bacteria that can cause shigellosis (dysentery). It was first isolated in 1896 in Japan by Kiyoshi Shiga. There are four subgroups of Shigella - S. dysenteriae, S. flexneri, S. boydii, and S. sonnei - classified based on biochemical and antigenic characteristics. Shigella is transmitted via the fecal-oral route and causes symptoms ranging from asymptomatic infection to severe dysentery. It produces Shiga toxin which is cytotoxic and responsible for the bloody diarrhea and tenesmus associated with shigellosis. Diagnosis involves culturing fresh stool samples on selective media like deoxycholate cit
Medical Microbiology Laboratory (Enterobacteriaceae - V)Hussein Al-tameemi
The document discusses the Enterobacteriaceae family of bacteria, including Yersinia and Morganella species. It notes that Enterobacteriaceae are gram-negative, facultatively anaerobic bacilli that are commonly found in the intestines of humans and animals. It provides classifications based on lactose fermentation and describes various media used to differentiate types. Specific details are given on Yersinia enterocolitica, Y. pseudotuberculosis, and Y. pestis. Information is also provided on the taxonomy, characteristics, infections, diagnosis and culture of Morganella morganii.
1. Enterobacteriaceae are a family of Gram-negative bacteria that are commonly found in the intestines of humans and animals. They include several important pathogens like Escherichia coli, Salmonella, Shigella, Klebsiella, and Enterobacter.
2. Members of Enterobacteriaceae are facultative anaerobes and most have peritrichous flagella. Some important genera include Escherichia, Salmonella, Shigella, Klebsiella, Citrobacter, Enterobacter, Proteus, Serratia, and Morganella.
3. Several species of Enterobacteriaceae can cause diseases like diarrhea, intestinal infections, urinary tract infections
This document discusses Enterobacteriaceae, a family of Gram-negative bacteria that includes many common pathogens. It provides details on their characteristics, identification, classification based on lactose fermentation, and important genera such as Escherichia coli. Reasons why E. coli is commonly used for gene cloning are described, including its genetic simplicity, rapid growth, safety, extensive prior study, and ability to host foreign DNA. Identification of Enterobacteriaceae involves examining biochemical reactions and growth on selective media like MacConkey agar.
Staphylococcus is a genus of gram-positive cocci that commonly causes infections in humans. Staphylococcus aureus is one of the most important pathogenic species, able to produce several toxins and enzymes that contribute to its virulence. These include hemolysins, leukocidins, enterotoxins, toxic shock syndrome toxin, and exfoliative toxins. S. aureus can cause a variety of infections through cutaneous, respiratory, or deep tissue routes. Laboratory identification involves gram stain morphology, culture characteristics such as catalase and coagulase production, and biochemical testing. Methicillin-resistant S. aureus is an important antibiotic resistant variant.
This document discusses Escherichia coli (E. coli), a common bacterium found in the intestines of humans and animals. It describes E. coli's morphology, identification, and ability to cause diseases like urinary tract infections, neonatal meningitis, and intestinal diseases. Prevention methods are outlined, including good hygiene practices like handwashing and thoroughly cooking meats to avoid transmission. Treatment focuses on fluid replacement rather than antibiotics in most cases unless the disease has progressed to a systemic stage.
Diagnosis of fungal disease by Dr. Manoj karkimanojj123
Early diagnosis of fungal infection is critical for effective treatment. History, clinical signs, gross pathology and in few cases intradermal skin test are all of the value in the diagnosis of clinical specimens.
This document provides information about Gram positive bacilli (rods), specifically the genus Clostridium. It discusses the characteristics of Clostridium including that they are spore-forming, anaerobic, Gram-positive rods. It also describes several medically important Clostridium species (C. tetani, C. botulinum, C. perfringens, C. difficile), their samples and microscopic features, culture characteristics, biochemical tests for identification, and a simple differentiation chart comparing characteristics. The document is authored by Hussein A. Abid, a medical laboratory scientist and chairman of the Iraqi Medical Laboratory Association.
This document discusses Salmonella, including its isolation, morphology, cultural characteristics, biochemical activities, epidemiology, virulence factors, pathogenesis, clinical diseases, laboratory diagnosis, and treatment. Salmonella was first isolated in 1885 and causes diseases like typhoid fever, paratyphoid fever, and foodborne illness in humans. It is a gram-negative rod found in the intestines of animals and can contaminate foods. Diagnosis involves culturing specimens from blood, feces, urine or vomit. Treatment includes antibiotics like chloramphenicol, ampicillin, and ciprofloxacin.
Shigella dysenteriae is a gram-negative, non-motile bacillus that causes dysentery. It grows well at 37°C in nutrient broth, forming colorless colonies on MacConkey agar and red colonies without black centers on XLD agar. S. dysenteriae infection is caused by ingesting contaminated food or water and leads to dysentery characterized by bloody mucus in stool. Laboratory diagnosis involves examining stool samples microscopically for pus cells and macrophages, culturing on selective media to isolate non-lactose fermenting colonies, and conducting biochemical tests and slide agglutination with specific antisera. Tetracycline and chloramphenicol are used
This document provides information on the bacteria Neisseria, including its classification, properties, species of medical importance (N. gonorrhoeae and N. meningitidis), laboratory diagnosis, treatment, and prevention. It discusses the pathogenesis, virulence factors, and diagnosis of N. meningitidis which causes meningitis, and N. gonorrhoeae which causes gonorrhea. Methods for isolating and identifying these bacteria in the laboratory are also summarized.
Bacillus is a genus of rod-shaped, Gram-positive bacteria that can form dormant endospores. The document focuses on Bacillus anthracis, which causes anthrax. It describes the morphology, cultural characteristics, virulence factors, and methods of diagnosis and prevention of B. anthracis. Key points include that B. anthracis forms encapsulated, non-motile rods and terminal spores. The anthrax toxins are composed of lethal factor, edema factor, and protective antigen, which combine to cause disease. Diagnosis involves microscopy, culture, and serology. Prevention for humans involves vaccination with anthrax toxoid and occupational hygiene, while animals are vaccinated with attenuated spore
The document discusses various bacterial infections that can cause diarrhea, including salmonellosis, typhoid fever, paratyphoid fever, non-typhoidal salmonella infections, shigellosis, cholera, and escherichiosis. It describes the causative organisms, pathogenesis, clinical presentation, diagnosis, and treatment of each infection. Key points include that salmonella can cause gastroenteritis, typhoid fever is caused by S. typhi, paratyphoid fever is milder illness caused by S. paratyphi, shigella causes dysentery, V. cholerae toxin causes cholera's profuse diarrhea, and E. coli strains like ETEC, EPE
This document provides an overview of Salmonella, including Salmonella enterica and Salmonella bongori. It discusses Salmonella serotyping based on surface structures. The pathogenesis and immunity of Salmonella is described, noting how it attaches and invades the intestines. Two pathogenicity islands regulate these processes. Epidemiology sections explain the animal reservoirs and most common sources of human infections like poultry, eggs and dairy. Clinical diseases caused include gastroenteritis, septicemia, enteric fever and asymptomatic colonization. Laboratory diagnosis focuses on culturing Salmonella from blood, feces or bone marrow. Biochemical tests are used to identify isolates.
Shigella bacteria cause the infectious disease shigellosis or bacillary dysentery. It is estimated to cause around 90 million cases annually, resulting in over 100,000 deaths mostly in children in developing countries. The bacteria are highly contagious and can be transmitted through contaminated food or water. Symptoms include bloody diarrhea, abdominal cramps, and fever. Treatment involves rehydration and antibiotics to reduce duration of symptoms.
This document discusses Salmonellosis, caused by the Salmonella bacterium. It affects various warm-blooded animals and has two main clinical presentations: enteritis, characterized by diarrhea and enterocolitis; and septicemia, a systemic infection most common in young animals. The bacterium is transmitted orally through contaminated food, water or feces. Control relies on reducing exposure through biosecurity, sanitation and antibiotic treatment of clinical cases.
prof . dr. ihsan edan alsaimary
department of microbiology - college of medicine - university of basrah - basrah -IRAQ
ihsanalsaimary@gmail.com
00964 7801410838
This document provides information on non-typhoidal salmonellosis (NTS) for 5th year medical students. It describes the salmonella nomenclature system, epidemiology of NTS including common sources of infection, pathophysiology involving intestinal inflammation, clinical manifestations such as diarrhea and potential complications, diagnosis through stool and blood cultures, and treatment focusing on rehydration and antibiotic use only for severe cases. Prevention emphasizes reducing bacterial contamination of foods, improving food safety, and hygienic measures.
Salmonella & Vibrio Cholerae an overview.pdfAlanShwan2
medical Bacteriology
Salmonella & Vibrio
Salmonella infection (salmonellosis) is a common bacterial disease that affects the intestinal tract. Salmonella bacteria typically live in animal and human intestines and are shed through stool (feces). Humans become infected most frequently through contaminated water or food.
Some people with salmonella infection have no symptoms. Most people develop diarrhea, fever and stomach (abdominal) cramps within 8 to 72 hours after exposure. Most healthy people recover within a few days to a week without specific treatment.
Cholera is a bacterial disease usually spread through contaminated water. Cholera causes severe diarrhea and dehydration. Left untreated, cholera can be fatal within hours, even in previously healthy people.
Modern sewage and water treatment have virtually eliminated cholera in industrialized countries. But cholera still exists in Africa, Southeast Asia and Haiti. The risk of a cholera epidemic is highest when poverty, war or natural disasters force people to live in crowded conditions without adequate sanitation.
This document summarizes key information about foodborne infections. It discusses Salmonella, which has over 2000 serotypes including S. typhi and S. paratyphi A, B, C. It also discusses reservoirs, transmission, clinical presentation, and prevention of various foodborne illnesses caused by Salmonella, Staphylococcus aureus, Bacillus cereus, Clostridium perfringens, Clostridium botulinum, and Vibrio cholerae. General preventive measures are outlined such as proper food handling, processing, and sterilization to prevent transmission of bacteria and toxins through contaminated food and water.
This document provides an overview of enteric infections. It discusses that diarrheal diseases are the third most common cause of medical visits in the US and the leading cause of childhood death globally. It outlines the various host, location, and timing factors that influence enteric infections. It then describes the major bacterial, viral and parasitic pathogens that cause acute and persistent diarrhea, including their modes of transmission, clinical presentations, and potential complications.
This document discusses Salmonella bacteria, which can cause typhoid fever and gastroenteritis in humans. It covers the taxonomy and pathogenesis of Salmonella, describing how the bacteria are ingested and can disseminate throughout the body to cause systemic infection. Key aspects of typhoid fever such as symptoms, epidemiology, diagnosis and treatment are summarized. The document provides detailed information on Salmonella and typhoid fever.
1. The document discusses the anatomy and physiology of the digestive tract, focusing on the pathogens Salmonella, Shigella, and Yersinia.
2. It describes the characteristics and pathogenesis of Salmonella and Shigella, including their ability to invade the intestinal epithelium and cause diseases like gastroenteritis and dysentery.
3. The treatment and prevention of infections from these pathogens involves controlling contamination, identifying carriers, and using antibiotics in some cases. Proper food preparation helps control the spread of Salmonella.
Salmonellae, causing agents of food Toxin infectionsEneutron
Salmonellae bacteria are the causative agents of foodborne infections. There are over 20 species and types of Salmonella that can cause disease in humans. Salmonella are transmitted through contaminated food, especially meat, eggs, and water. Infection results in a variety of gastrointestinal symptoms like diarrhea, fever, and abdominal cramps. Laboratory techniques are used to diagnose Salmonella infections through culturing samples and identifying the bacteria. Treatment involves antibiotics while prevention focuses on food safety practices and controlling Salmonella in animals, food processing facilities, and carriers.
Chapter three fecal oral transmitted diseasesAhmed Batun
This chapter discusses diseases transmitted through the fecal-oral route. It identifies five diseases in particular: typhoid fever, bacillary dysentery (shigellosis), amoebiasis, giardiasis. For each disease, it provides details on the infectious agent, epidemiology including transmission and clinical presentation. The key aspects are diagnosis based on identifying the causative agent in stool samples and treatment involving antibiotics or antiparasitic drugs while prevention emphasizes good hygiene, safe water and proper sanitation.
The document lists various diseases including typhoid, rabies, tuberculosis, cholera, jaundice, chickenpox, diarrhea, goiter, cancer, and polio. It then provides more detailed descriptions of typhoid fever, rabies, tuberculosis, cholera, jaundice, chickenpox, diarrhea, goiter, and cancer explaining their causes, symptoms, transmission, treatments, and global impacts.
This document discusses amoebiasis, a common intestinal infection caused by the parasite Entamoeba histolytica. It has a worldwide distribution and is a major health problem in areas with poor sanitation. Symptoms range from mild diarrhea to severe dysentery and liver abscesses. Diagnosis is made by identifying the parasite in stool samples. Treatment involves drugs like metronidazole. Prevention relies on improved sanitation, safe food and water, health education, and treatment of carriers like food handlers.
Enteric fever, also known as typhoid fever, is caused by Salmonella Typhi and Paratyphi bacteria. It is most common in developing countries with poor sanitation and is spread through contaminated food or water. The disease causes systemic infection throughout the body and symptoms include sustained fever, abdominal pain, headaches and rash. Complications can include intestinal bleeding, perforation or neurological manifestations if left untreated.
Enteric fever, also known as typhoid fever, is caused by Salmonella enterica serovars Typhi and Paratyphi. It is a systemic illness characterized by prolonged fever and abdominal symptoms. While it has declined in developed countries, it remains common in developing areas with poor sanitation. The disease spreads through ingestion of food or water contaminated by infected human feces or urine. Complications can include intestinal bleeding, perforation and neurologic manifestations. Diagnosis is made through culturing the infecting Salmonella from blood, bone marrow, stool or other tissues.
Salmonellosis is caused by Salmonella bacteria and is a major cause of foodborne illness worldwide. There are over 2,500 serotypes of Salmonella but less than 100 cause infections in humans. Salmonella enterica serovar Typhi and Paratyphi cause enteric fever, resulting in systemic illness with symptoms like sustained fever. Nontyphoidal Salmonella generally cause self-limiting gastroenteritis. Diagnosis is made by isolating the bacteria from stool culture. Treatment involves rehydration and sometimes antibiotics. Prevention relies on safe food/water handling and hygiene practices.
Similar to Enumeration of salmonella and shigella (20)
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2. Introduction
Salmonellosis is a bacterial disease commonly manifested by an acute
enterocolitis, with sudden onset of headache, abdominal pain,
diarrhea, nausea and sometimes vomiting.
Deaths are uncommon, except in the very young, in the very old, the
debilitated and immunosuppressed. However, morbidity and
associated costs of salmonellosis may be high.
Salmonellosis is classified as a food borne disease, because
contaminated food, mainly of animal origin, is the predominant mode
of transmission.
Epidemiologically, Salmonella gastroenteritis's may occur in small
outbreaks in the general population.
3. However, large outbreaks in hospitals, institutions for children,
restaurants are not uncommon and usually arise from food
contaminated at its source, or, less often, during handling by an ill
person or a carrier, but person-to-person spread can occur.
They cause illnesses in humans and many animals, such as typhoid fever
and enteritis. Salmonella (e.g. Salmonella enterica subsp. enterica serovar
enteritidis) can cause diarrhea.
According to the World Health Organization over 16 million people
worldwide are infected with typhoid fever each year, with 500,000 to
600,000 of these cases proving to be fatal.
4. • A large outbreak of Shigella sonnei gastroenteritis occurred in Murcia
Region (Southeast Spain) in the winter of 1995–1996. More than 200
people were affected.
• Epidemiological investigations implicated a regionally manufactured
fresh pasteurized milk cheese as the vehicle of infection.
• The dispersed sale of the cheese resulted in a regional dissemination of
the organism and people were affected in eight townships.
5. • The higher susceptibility in young children of contracting
Shigellosis and typhoid fever in addition to the high
prevalence of Salmonella and Shigella—found to grow
rapidly in liquid infant formula—has focused the attention of
the scientific community to study the survival capabilities of
these organisms in foods.
• In addition, the wide distribution of this commodity
throughout the world creates the risk of a bioterrorism attack
directed against the infant population.
6. Transmission and Source of Infection of
Salmonella
Salmonella infections are zoonotic
Salmonella is usually transmitted to humans by eating foods
contaminated with animal faeces.
Contaminated foods usually look and smell normal.
Foods of animal origin, such as beef, poultry, milk, or eggs, but any
food, including vegetables, may become contaminated.
Food may also become contaminated by the hands of an infected food
handler who did not wash hands with soap after using the bathroom.
Salmonella may also be found in the feaces of some pets, especially
those with diarrhoea, and people can become infected if they do not
wash their hands after contact with pets or pet feaces.
7. Salmonella
Salmonella spp. do not ferment lactose.
Most species of Salmonella are motile with peritrichous flagella.
Some Salmonellae have capsular antigens; that of S. Typhi is referred to
as Vi antigen.
Groups and species of Salmonella are identified by serologic analysis of
O and H antigens (> 2,500 serotypes). Classification of salmonellae is
traditionally based on serogrouping and serotyping (e.g. S. typhimurium,
which is reclassified as S. enterica together with most human pathogens
by analysis of DNA homology). The correct name for S. typhi is S.
enterica, serovar. Typhi or S. Typhi. They can be identified by
biochemical tests and serogrouping, with follow-up serotyping
confirmation.
8. Salmonella
Epidemiology
S. Typhi and S. Paratyphi are primarily infective for humans.
Other salmonellae are chiefly pathogenic in animals (poultry, pigs,
rodents, cattle, pets etc.) that constitute the reservoir for human
infection.
Humans usually become infected by ingestion of contaminated food or
drink (mean infective dose: 106
-108
, but that of S. typhi is lower). In
children, infections can result from direct fecal-oral spread.
The most common sources of human infections: poultry, eggs, dairy
products, and foods prepared on contaminated work surfaces. However,
the major source of infection for enteric fever is the carriers (convalescent
or healthy permanent).
9. Salmonella
Pathogenesis and Immunity
Invasion
Acid tolerance response (ATR) gene protects the organism from
gastric acid.
The bacteria invade into (by inducing membrane ruffling) and multiply
in the M cells and enterocytes of the small intestine. They can also be
transported across the enterocytes and released into the blood and
lymphatic circulation.
Inflammatory response confines the infection to the GI tract in non-
typhoid salmonellosis.
Survival in macrophages
Salmonellae are facultative intracellular pathogen.
10. Salmonella
Clinical diseases
1. Enteritis
Incubation period: 6-48 hours.
Symptoms: nausea, headache, vomiting, non-bloody profuse diarrhea,
with few leukocytes in the stools. Low-grade fever, abdominal cramp,
myalgia, and headache are also common.
Episode resolves in 2-7 days.
Inflammatory lesions of the small and large intestine are present. Stool
cultures remain positive for several weeks after clinical recovery.
11. Salmonella
Clinical diseases
2. Bacteremia
Most common causal species: S. Choleraesuis, S Typhi and S. Paratyphi.
Symptoms: like sepsis caused by other gram-negative bacteria. 10% of
patients may have localized suppurative infections, e.g., osteomyelitis,
endocarditis, arthritis, etc.
High risk population: pediatric and geriatric patients; AIDS patients.
12. Salmonella
Clinical diseases
3. Enteric fever (typhoid fever)
Causal species: S. Typhi, S. Paratyphi A, S. Schottmuelleri,
and S. Hirschfeldii.
Mouth small intestine lymphatics and bloodstream
infect liver, spleen and bone marrow
multiply and pass into the blood second and heavier
bacteremia onset of clinical illness
colonization of gallbladder invasion of the
intestine typhoid ulcers and severe illness.
Chronic carriers (1%-5% of patients): bacteria persist in the
gallbladder and the biliary tract for more than one year.
13. Symptoms: incubation time: 10-14 days. Gradually increasing fever,
malaise, headache, myalgias, and anorexia, which persist for a week
or longer.
In severe cases: intestinal hemorrhage and perforation.
Principal lesions: hyperplasia and necrosis of lymphoid tissue,
hepatitis, focal necrosis of the liver, and inflammation of the
gallbladder, periosteum, lungs and other organs.
14. Salmonella
Treatment
Enteric fever and bacteremia require antibiotic treatment:
chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole.
Surgical drainage of metastatic abscesses may be required.
Salmonella enterocolitis needs only supportive therapy (antibiotic
treatment may prolong the symptoms and excretion of the
salmonellae). Drugs to control hypermotility of the gut should be
avoided because it is easy to transform a trivial gastroenteritis into
a life-threatening bacteremia by paralyzing the bowel.
Chronic carriers of S. Typhi may be cured by antibiotics alone or
combined with cholecystectomy.
15. Salmonella
Prevention and control
Sanitary measures.
Carriers must not be allowed to work as food handlers.
Strict hygienic precautions for food handling.
Vaccines against S. Typhi:
Purified Vi antigen
Oral, live attenuated vaccine.
16. Shigella
S. dysenteriae, S. flexneri , S. sonnei , & S. boydii: bacillary dysentery
> 45 O serotypes; have no H antigen; do not ferment lactose.
Pathogenesis and Immunity
Shigellosis is primarily a pediatric disease, and is restricted to the GI tract.
Mean infective dose: 103
.
Mouth colon invade M cells and subsequently spread to mucosal
epithelial cells cause microabscess in the wall of colon and terminal
ileum necrosis of the mucous membrane, superficial ulceration,
bleeding, and formation of pseudomembrane.
Shiga toxin
An A-B toxin inhibiting protein synthesis.
Damages intestinal epithelium and glomerular endothelial cells (associated
with HUS) .
17. Internalized shigellae induce
apoptosis of macrophage and
release of the bacteria
Attracted by the
cytokines
released by
macrophage
Destablize the
intestinal wall
Activates the invasion genes on
the virulence plasmid
M cell
18. Shigella
Clinical diseases
Incubation period: 1-3 days
Sudden onset of abdominal pain, fever and watery diarrhea
number of stools increase, less liquid, often contain mucus
and blood, rectal spasms with resulting lower abdominal pain
(tenesmus) symptoms subside spontaneously in 2-5 days in
adult cases, but loss of water and electrolytes frequently occur in
children and the elderly a small number of patients remain
chronic carriers.
Some cases were accompanied by hemolytic uremic syndrome
(HUS).
19. Shigella
Laboratory diagnosis
Specimens: fresh stool, mucus flecks, and rectal swabs. Large
numbers of fecal leukocytes and some RBC may often be seen
microscopically.
Culture: differential and selective media as used for salmonellae.
Treatment
Antibiotic treatment: chloramphenicol, ampicillin, tetracycline, and
trimethoprim-sulfamethoxazole. Drug resistance is common.
Opiates should be avoided.
20. Shigella
Prevention and control
Humans are the only reservoir for shigellae.
Transmission of shigellae: water, food, fingers, feces, and flies.
Most cases occur in children under 10 years of age.
Prevention and control of dysentery:
1. Sanitary control of water, food and milk; sewage
disposal; and fly control.
2. Isolation of patients and disinfection of excreta.
3. Detection of subclinical cases and carriers.
21. Isolation and enumeration principle
Pre-enrichment
Twenty-five (25) grams or ml of sample is added to 225 ml of
buffered peptone water and incubated at 37°C for 24 hours.
Selective Enrichment
Transfer one ml portion from pre-enrichment step to each 10 mL of
selenite eosine broth and tetrathionate broth and incubated at 37°C
for overnight.
Selective plating
Then the contents of both tubes were mixed and a loopful was
streaked on to the xylose lysine deoxycolate agar (XLDA), and
bismuth sulphite agar (BSA) plate and Hektoen enteric agar (HEA).
These plates were incubated at 37°C for 24 hrs. The incubation may
be continued up to 72 hrs before report as nil.
22. Xylose lysine deoxycolate (XLDA) agar
Lactose, Sucrose, and Xylose are the fermentable carbohydrates present
and phenol red is used as the pH indicator.
Bacteria that ferment none of these sugars, e.g., Shigella, appear as red,
translucent colonies
Yellow colonies indicate a rapid fermentation of lactose and acid pH, as
demonstrated by E. coli
Organisms that ferment xylose as well as decarboxylate lysine exhaust
the xylose rapidly and the lysine reaction causes a pH reversal to the
alkaline reaction similar to Shigella
Sodium thiosulfate and ferric ammonium citrate are indicators of H2S
production only when alkaline conditions exist
23. • Salmonella will, therefore, form red colonies with black
centre in 24 hrs
• Sodium deoxycolate is added to inhibit gram-positive
growth and to retard the growth of many strains of coli
forms
Pink and black color colony of Salmonella on XLD agar
24. Bismuth sulphite agar (BSA)
• Brown, grey, or black colonies; sometimes they have a metallic
sheen.
• In this medium freshly precipitated bismuth sulphite acts together
with brilliant green as a selective agent by suppressing the growth
of coliforms, whilst permitting the growth of Salmonellae.
• Sulphur compounds provide a substrate for hydrogen sulphide
production, whilst the metallic salts in the medium stain the
colony and surrounding medium black or brown in the presence
of hydrogen sulphide.
26. Hektoen Enteric Agar (HEA)
HEA is used for isolating and differentiating enteric pathogens such as
Salmonella, Shigella and other Gram-negative Enterobacteriaceae.
The nutrients for growth are provided by the meat, peptone and yeast
extract.
The increased content of the peptone and the three fermentable
carbohydrates (lactose, sucrose, salicin) as sources of carbon and energy
reduce the inhibitory action of the bile salts on Salmonella and Shigella
spp.
Bromo-thymol blue and acid fuchsin are pH indicators.
27. Sodium thiosulphate provides sulphur and ferric ammonium citrate is
the indicator for H2S production.
H2S positive colonies are blue-green to blue colonies with or without
black center
Blue-green to bluish colony of Salmonella on HEA
28. RAMBACH Agar (chromogenic medium)
for Salmonella
• Differential diagnostic culture medium for identifying non-typhi
Salmonella in foodstuffs and clinical samples
• Sodium deoxycolate inhibits the accompanying Gram-positive flora.
RAMBACH Agar enables species of Salmonella to be differentiated
unambiguously from other bacteria
• Propylene glycol to the culture medium. Salmonellae form acid with
propylene glycol, so that, in combination with a pH indicator, the
colonies have a characteristic red color
• Differentiate coliforms from Salmonellae, the medium contains a
chromogen indicating the presence of ß-galactosidase
29. Coliform microorganisms grow as blue-green or blue-violet colonies. Other
Enterobacteriaceae and Gram-negative bacteria, such as Proteus,
Pseudomonas, Shigella, S. typhi and S. parathyphi grow as colorless to yellow
colonies.
Red color colony of Salmonella on RAMBACH agar
30. Triple Sugar Iron (TSI) Agar
• Ferrous sulfate
• Sodium thiosulfate
• Sodium chloride
• Agar (1.2%)
• Phenol red
• pH = 7.4
31. TSI Reactions of the Enterobacteriaceae
• Yellow deep, purple slant: acid deep due to glucose fermentation,
no lactose or sucrose fermentation with alkaline slant due to
production of amine’s from protein
• Black deep, purple slant: acid deep due to glucose fermentation
with H2S production, no lactose or sucrose fermentation
• Yellow deep and slant: acid deep and slant due to glucose as well
as lactose and/or sucrose fermentation
• Black deep and yellow or black slant: acid deep and slant with
glucose and lactose and/or sucrose fermentation with H2S
production
• Fracturing or lifting of agar from base of culture tube: CO2
production
32. Triple sugar iron test
Reaction Fermentation
Acid butt (yellow), alkaline Slant (red) Glucose fermented
Acid throughout medium, butt and slant yellow both
fermented
Lactose or Sucrose or both fermented
Gas bubbles in butt, medium sometimes split Aerogenic culture
Blackening of the butt Hydrogen sulphide Produced
Alkaline slant and butt (medium entirely red) None of the three sugars fermented
33. Biochemical reactions of Salmonella and Shigella
Test or substrate
Result
Salmonella
species
reaction
Shigella
species
reaction
Positive Negative
Glucose (TSI) Yellow Butt Red Butt + -
Lysine decarboxylase (LIA) Purple butt Yellow butt + -
H2S (TSI) Blackening No blackening + -
Urease Purple-red color No color change - -
Phenol red dulcitol broth Yellow color and/ or gas No gas; no color
change
+ -
KCN broth Growth No growth - +
Malonate broth Blue color No color change - +
Indole test Violet color at surface Yellow color at
surface
- ±
Phenol red lactose broth Yellow color and/ or gas No gas; no color
change
- -
Phenol red sucrose broth Yellow color and/ or gas No gas; no color
change
- -
Voges-Proskauer test Pink-to-red color No color change - -
Methyl red test Diffuse red color Diffuse yellow color + +
Citrate utilization + +
p-phenyl Pyruvic acid - -
34. Urease Test
Ability of microorganism to degrade urea in the presence Urease
enzyme produced by bacteria and is indicated by phenol red
indicator which undergoes color change from peach to pink.
If on streaking a Urease agar slant and on incubating it at 37°C for
24 hrs color changes to pink the bacteria is Urease positive