This document summarizes a study that compared the resistance to Fusarium wilt disease in seeded and regenerated sesame plants. The researchers established a system for regenerating sesame plants through tissue culture using different plant growth regulators, which resulted in somaclonal variation. Regenerated and seeded plantlets were compared for resistance after being inoculated with Fusarium oxysporum fsp sesami. Seeded plantlets displayed signs of susceptibility and died within 20 days, while regenerated plantlets resisted infection and continued growing despite being smaller and weaker initially. The results demonstrated that somaclonal variation through tissue culture induced resistance to Fusarium wilt in regenerated sesame plantlets.
— This study summarizes the results of 30 years of our experiments with Vicia faba L seeds. Our long-term practical observations of different Vicia faba L. cultivars points out the method useful for the higher yield of seeds in terms of their viability and thus higher crop production. Our experiments led to the following important findings regarding of seed viability: 1. Individual and group variability of seeds; 2. Storage condition before germination; and 3. The condition of their germination. All these three influential conditions is possible to optimalize by method of storage effect described in this our report resulting in the improvement of crop production. This is especially important in case of seeds that are rare and/or expensive, i.e. seeds that are genetically modified or with rearranged karyotypes. Keywords— seed color, higher germination, improvement of viability, higher crop production.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
Effect of Different Concentrations of Glycerol on survival of Azotobacter chr...IIJSRJournal
The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 25 mM in the medium containing glycerol (38.66 colonies on zero day and 22 colonies on 360th day) (109 CFU/ml).
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
Introduction
Advantages of Micropropagation over the conventional methods
History
Stages of Micropropagation
1. Stage 0; Preparative stage
2. Stage 1; Initiation of aseptic cultures
A) Explant
B) Sterilization
C) Browning of medium
Factors affecting initiation stage
Conclusions
References
— This study summarizes the results of 30 years of our experiments with Vicia faba L seeds. Our long-term practical observations of different Vicia faba L. cultivars points out the method useful for the higher yield of seeds in terms of their viability and thus higher crop production. Our experiments led to the following important findings regarding of seed viability: 1. Individual and group variability of seeds; 2. Storage condition before germination; and 3. The condition of their germination. All these three influential conditions is possible to optimalize by method of storage effect described in this our report resulting in the improvement of crop production. This is especially important in case of seeds that are rare and/or expensive, i.e. seeds that are genetically modified or with rearranged karyotypes. Keywords— seed color, higher germination, improvement of viability, higher crop production.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
Effect of Different Concentrations of Glycerol on survival of Azotobacter chr...IIJSRJournal
The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 25 mM in the medium containing glycerol (38.66 colonies on zero day and 22 colonies on 360th day) (109 CFU/ml).
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
Introduction
Advantages of Micropropagation over the conventional methods
History
Stages of Micropropagation
1. Stage 0; Preparative stage
2. Stage 1; Initiation of aseptic cultures
A) Explant
B) Sterilization
C) Browning of medium
Factors affecting initiation stage
Conclusions
References
Ultrastructural and physiological aspects of eucalyptus clones submitted to b...Innspub Net
This work aimed evaluate ultrastructural and physiological aspects of 35-days old eucalyptus, clones 13; 59 and 131, by submitting them to the following barium doses: null, 125; 250; 500 and 1000 ppm of barium solution, with four repetitions. Due to the exposition to barium, there was appearance of phytotoxicity on the leaves. Then, it was determined the following variables: Phloem of Diameter of Leaf and Root (PDL and PDR); Xylem of Diameter of Leaf and Root (XDL and XDR) and Thickness of Palisade Parenchyma (PP); Spad Index (SPADI); Inferior Face of Stomata Functionality of the Inferior Face (IFSF) and Stomach Density of the Inferior Face (SD). Barium negatively influenced the leaf ultrastructure of eucalyptus and its physiology. Eucalyptus clones 13; 59 and 131 are susceptible to barium solution. Barium caused root and ultrastructural leaves and root damages on eucalyptus clones. Xylem diameter of leaves of eucalyptus clones 13 and 59 were not harmed as exposed to Barium.
Seed Setting Efficiency, Leaf Relative Water Content and Yield Traits of some...Premier Publishers
A potted experiment was conducted in the screen house between February and April, 2017. Seeds of five cowpea varieties were obtained and placed in plastic buckets of 7-liter capacity, perforated at the bottom and filled with top soil. Three soil moisture level treatments of Severe Stress (SS), Mild Stress (MS) and Non-Stress (NS) conditions were imposed. Pots were weighed in the morning of each day for the period of eight weeks to obtain daily cumulative water transpired. The experiment was a 5 x 3 factorial combination arranged using completely Randomized Design with six replications. Some of the Growth and Yield indices measured were, Leaf Relative Water Content (%), Seed Setting Efficiency (Ef), Crop Growth Rate (CGR), Pod Growth Rate (PGR), Water use efficiency (WUE), and Seed Yield (kg/ha). From the study, water stress negatively affects leaf relative water content, seed setting efficiency and cowpea yield by inducing reproductive organ abortion during reproductive development of cowpea. Consequently, the crop seed setting efficiency is lowered. Poor seed yield of cowpea under the severe water stress condition occurred as a result of reduced rate of assimilates production caused by water stress which reduced water potential in cowpea leaf and pods. Varieties exhibiting higher (WUE) were recorded more at non stress level and this was recorded for all the varieties except for Oloyin Brown which had higher (WUE) under mild-stress condition. At severe water stress conditions, most of the varieties recorded lower (WUE) except in varieties IT98K-503-1. Water stress reduced leaf relative water content and grain yield of cowpea. Among the varieties evaluated, IT98K-503-1 performed better at Non stress condition with higher seed setting efficiency (1.94), leaf relative water content (86.9%), and seed yield (14.8kg/ha).
Isolation and characterization of plant growth promoting bacteria (PGPB) from...Agriculture Journal IJOEAR
— The use of anaerobic digestate as fertilizer is considered beneficial since it provides plant nutrients and organic matter to soils. However, there is limited information about plant growth promoting bacteria (PGPB) in digestate. In this study, we isolated Bacillus and Pseudomonas from two types of anaerobic digestates, and selected three different plant growth promoting traits and antifungal activity to screen 200 bacteria isolated from each digestate. Then 6 isolates based on plant growth promoting traits were selected and inoculated with common wheat seeds to evaluate their plant growth promoting activity. Cultivable population of Bacillus and Pseudomonas were 2.20 × 10 6 and 6.98 × 10 4 CFU g-1 dry matter in mesophilic digestate, while were 6.86 × 10 5 and 5.65 × 10 4 CFU g-1 dry matter in thermophilic digestate. Twenty-five bacterial isolates from mesophilic digestate and 12 bacterial isolates from thermophilic digestate showed positive plant growth promoting traits or antifungal activity. In plant growth promoting assay, all isolates significantly promoted growth of wheat seedlings (p < 0.05). Seedlings stem length was increased from 28.5% to 38.6% by bacteria inoculation. In addition, bacteria inoculation increased seedlings stem weight from 113.3% to 214.2% and root weight from 108.6% to 207.2% as compared to un-inoculated control. The results showed that anaerobic digestate was a potential source for isolation of PGPB, and PGPB in digestate would be beneficial for plant growth with fertilizer application.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
Resistance/tolerance to root-knot nematode Meloidogyne incognita (Kofoid and ...Premier Publishers
This experiment was carried out in a Randomized Complete Block Design (40 X 10m plot) to evaluate cacao clones for resistance and tolerance against Meloidogyne incognita in infested field. The ten cocoa clones evaluated are MXC67, T86/2, PA150, LCTEEN, T12/11, T53/5, T101/15, T65/7, ICS1 and AMAZ 15-15. Pre-planting survey revealed ten genera of phytonematodes, these were; Meloidogyne spp., Pratylenchus spp., Helicotylenchus spp. Paralongidorus spp., Eutylenchus spp., Scutellonema spp., Hemicyclophora spp., Xiphinema spp., Longidorus spp., Anguillulina spp., Psilenchus spp., and Tetylenchus spp. Meloidogyne spp. had the highest frequency of occurrence and highest population in 250g soil (40 and 28,234 respectively), Paralongidorus spp. was next in population. Based on gall index, nematode reproduction factor and growth parameters, MXC67, T86/2, PA150, T101/15 and T53/5 were susceptible to the nematode. Two other clones, T65/7 and ICS1 were tolerant. A high degree of resistance was exhibited by LCTEEN, T12/11 and AMAZ 15-15. Compared with F3 Amazon and Amelonado varieties, the two most famous cocoa varieties in Nigeria, four clones (LCTEEN, T65/7, ICSI and AMAZ 15-15) were superior to F3 Amazon and Amelonado. This study showed that planting of resistance/tolerant cacao clones in nematode infested soil will drastically reduce seedling failure experienced by farmers. This tolerant and resistant clones identified should be included in breeding programme for resistance.
In vitro regeneration of multiple shoots from encapsulated somatic embryos of...IOSR Journals
A protocol has been established for plant regeneration from encapsulated somatic embryos of Artemisia vulgaris L. Encapsulated somatic embryo of A. vulgaris gave better response at 2% alginate, 75mM calcium chloride hardened for 30 minutes. Morphogenic response and percentage of conversion into plantlets of encapsulated beads on storage and temperature effect were studied. Further, encapsulated somatic embryo retrieved from storage conditions was evaluated for its viability. The encapsulated embryos can be stored upto 4 months at 40C, 200C and 220C. Maximum conversion frequency of 90% was observed from encapsulated somatic embryos cultured on MS medium supplemented with GA3 (1.5mg/l), IAA (0.5mg/l) and Ascorbic acid (40mg/l) and 22oC temperature was found to be optimum irrespective of storage periods. In the present investigation, the development of multiple shoots from a single synthetic seed is a recent record.
The experiment was conducted at the experimental farm and laboratory of Institute of
Sustainable Agrotechnology, University Malaysia Perlis, Padang Besar, Perlis, Malaysia, with the objective
toinvestigate the inhibitory and stimulatory effects of aqueous extract of mungbean on seed germination and
seedling growth of three crop species, mungbean, sweet corn and okra. Different treatments of mungbean
aqueousextracts (vegetative fresh, vegetative after 2 weeks drying, vegetative after 4 weeks drying, flowering
fresh, flowering after 2 weeks drying, flowering after 4 weeks drying, flowering fresh, flowering after 2 weeks
drying, flowering after 4 weeks drying, maturity fresh, maturity after 2 weeks drying, maturity after 4 weeks
drying and water as control) were used to test their effect on the test species. The experiment was randomly
distributed and according to Completely Randomized Design(CRD) with five replicates. The results showed the
fresh vegetative aqueous extract of mungbean had a significant effect (stimulatory) on germination percent and
growth parameters such as number of root, root length and shoot height, of the three crop species. The study
revealed that the aqueous extract of mungbean have different effects (inhibitory and stimulatory) on the
seedlings and the mode of action depends on the associated plant species.Our results suggest that the aqueous
extract of mungbean from the different growth stages and drying periods have an allelopathic effect.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Phosphate solubilizing potential of Rhizobium and Bacillus species for enhanc...Innspub Net
By International Network For Natural Sciences INNSPUB in Agronomy and Horticulture.
A field experiment was conducted to evaluate the separate and integrated effect of Rhizobium and Bacillus spp. on the growth of maize (Zea Mays L.). Inocula of Rhizobium and Bacillus were applied as seed coating. Recommended dose of fertilizer (120-60 kg NP ha-1) was applied at sowing. The treatments were implied according to Randomized Complete Block Design with three repeats. Inoculation had no significant effect on the leaf length (84cm) and internodal distance (18.3cm) compared to their respective control (80cm and16.5cm) but the photosynthetic rate (105.3μ mol-2s-1) , transpiration rate (13.2 mmolm-2s-1), plant height (259.3cm), leaf width (7.7cm), stem diameter (15.43mm), leaf area (644cm-2) and shoot fresh weight (79.6 tones ha-1) were significantly improved by co-inoculation. Effect of Bacillus was statistically at par with co-inoculation regarding transpiration rate (11.47 m mol m-2s-1), plant height (249.3 cm) and stem diameter (14.87 mm). Response of leaf width, stem diameter, leaf area and shoot fresh weight were significantly higher by Rhizobium application compared to the Bacillus inoculation, however, positive influence was observed by all the inoculation treatments over the control.These findings indicated that inoculation of Rhizobium and Bacillus has positive effect on the maize growth and their co-inoculation (Rhizobium+ Bacillus) showed more pronounced results. Get the full articles at: http://www.innspub.net/volume-4-number-1-january-2014-2/
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induc...Agriculture Journal IJOEAR
Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
Ultrastructural and physiological aspects of eucalyptus clones submitted to b...Innspub Net
This work aimed evaluate ultrastructural and physiological aspects of 35-days old eucalyptus, clones 13; 59 and 131, by submitting them to the following barium doses: null, 125; 250; 500 and 1000 ppm of barium solution, with four repetitions. Due to the exposition to barium, there was appearance of phytotoxicity on the leaves. Then, it was determined the following variables: Phloem of Diameter of Leaf and Root (PDL and PDR); Xylem of Diameter of Leaf and Root (XDL and XDR) and Thickness of Palisade Parenchyma (PP); Spad Index (SPADI); Inferior Face of Stomata Functionality of the Inferior Face (IFSF) and Stomach Density of the Inferior Face (SD). Barium negatively influenced the leaf ultrastructure of eucalyptus and its physiology. Eucalyptus clones 13; 59 and 131 are susceptible to barium solution. Barium caused root and ultrastructural leaves and root damages on eucalyptus clones. Xylem diameter of leaves of eucalyptus clones 13 and 59 were not harmed as exposed to Barium.
Seed Setting Efficiency, Leaf Relative Water Content and Yield Traits of some...Premier Publishers
A potted experiment was conducted in the screen house between February and April, 2017. Seeds of five cowpea varieties were obtained and placed in plastic buckets of 7-liter capacity, perforated at the bottom and filled with top soil. Three soil moisture level treatments of Severe Stress (SS), Mild Stress (MS) and Non-Stress (NS) conditions were imposed. Pots were weighed in the morning of each day for the period of eight weeks to obtain daily cumulative water transpired. The experiment was a 5 x 3 factorial combination arranged using completely Randomized Design with six replications. Some of the Growth and Yield indices measured were, Leaf Relative Water Content (%), Seed Setting Efficiency (Ef), Crop Growth Rate (CGR), Pod Growth Rate (PGR), Water use efficiency (WUE), and Seed Yield (kg/ha). From the study, water stress negatively affects leaf relative water content, seed setting efficiency and cowpea yield by inducing reproductive organ abortion during reproductive development of cowpea. Consequently, the crop seed setting efficiency is lowered. Poor seed yield of cowpea under the severe water stress condition occurred as a result of reduced rate of assimilates production caused by water stress which reduced water potential in cowpea leaf and pods. Varieties exhibiting higher (WUE) were recorded more at non stress level and this was recorded for all the varieties except for Oloyin Brown which had higher (WUE) under mild-stress condition. At severe water stress conditions, most of the varieties recorded lower (WUE) except in varieties IT98K-503-1. Water stress reduced leaf relative water content and grain yield of cowpea. Among the varieties evaluated, IT98K-503-1 performed better at Non stress condition with higher seed setting efficiency (1.94), leaf relative water content (86.9%), and seed yield (14.8kg/ha).
Isolation and characterization of plant growth promoting bacteria (PGPB) from...Agriculture Journal IJOEAR
— The use of anaerobic digestate as fertilizer is considered beneficial since it provides plant nutrients and organic matter to soils. However, there is limited information about plant growth promoting bacteria (PGPB) in digestate. In this study, we isolated Bacillus and Pseudomonas from two types of anaerobic digestates, and selected three different plant growth promoting traits and antifungal activity to screen 200 bacteria isolated from each digestate. Then 6 isolates based on plant growth promoting traits were selected and inoculated with common wheat seeds to evaluate their plant growth promoting activity. Cultivable population of Bacillus and Pseudomonas were 2.20 × 10 6 and 6.98 × 10 4 CFU g-1 dry matter in mesophilic digestate, while were 6.86 × 10 5 and 5.65 × 10 4 CFU g-1 dry matter in thermophilic digestate. Twenty-five bacterial isolates from mesophilic digestate and 12 bacterial isolates from thermophilic digestate showed positive plant growth promoting traits or antifungal activity. In plant growth promoting assay, all isolates significantly promoted growth of wheat seedlings (p < 0.05). Seedlings stem length was increased from 28.5% to 38.6% by bacteria inoculation. In addition, bacteria inoculation increased seedlings stem weight from 113.3% to 214.2% and root weight from 108.6% to 207.2% as compared to un-inoculated control. The results showed that anaerobic digestate was a potential source for isolation of PGPB, and PGPB in digestate would be beneficial for plant growth with fertilizer application.
Estimation of genetic parameters and gene actions of sorghum [Sorghum bicolor...Innspub Net
A study of sorghum tolerance to low P condition was conducted in two experiments : (1) in nutrient solution and
(2) in acid soil. The aim of this study was to obtain information on the genetic variability and heritability of agronomic characters that could be used as selection criteria in low P condition and to obtain information on gene action that controlled agronomic characters of sorghum.The plant materials used in this experiment were F2 segregating population derived from the cross between B69 and Numbu. The first experiment was conducted in the green house of Bogor Agricultural University. B69, Numbu and F2 population were grown hidroponically for 14 days. Composition of the nutrient solution used in the experiment followed the method suggested by Ohki with modification of P concentration. P is given in the form of KH2PO4: 0.001 mM. The results showed that shoot dry weight (SDW) and total dry weight (TDW) had high estimate of heritability and moderate genotypic
coefficient of variance (GCV), thus these characters can be used for criteria selection for low P tolerance at seedling stage. The significant value of skweness from the distribution shoot dry weight in F2 segregants, indicate the presence of complementary epistasis gene action, whereas normal distribution of total dry weight showed additive gene action. The second experiment was conducted in acid soils of Bogor District Experimental Field Tenjo, Bogor. The results showed that stem diameter, panicle weight and grain yield per plant had high heritability and wide GCV value, indicating these characters can be used as the selection criteria in selecting
sorghum genotypes for low P tolerance in acid soil. Based on the skewness values, all of the agronomy characters
observed in this study were influenced by additive gene action. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Effect of interaction between different plant growth regulators on in vitro s...Agriculture Journal IJOEAR
— In this paper a shoot multiplication is described for Citrus latifolia Tan. (persian lime) using nodal segment explants of young one – old – year trees by two different pathways contain with and without callusing phase. The best result for multiple shoot formation and regenerated shoot formation was 3.2 and 2.6 shoots per explants with 4.44 µM BA plus 0.053 µM NAA and 4.44 µM BA plus 0.049 µM IBA respectively. Alike shoot regeneration, shoot elongation was occurred in medium with 4.44 µM BA and 0.049 µM IBA. Micropropagated and regenerated plants are under other experiments. Abbreviation: BA – 6 benzylaminopurine; IBA – Indole acetic acid; NAA – Naphtalene acetic acid; PGRs – Plant Growth Regulators.
Resistance/tolerance to root-knot nematode Meloidogyne incognita (Kofoid and ...Premier Publishers
This experiment was carried out in a Randomized Complete Block Design (40 X 10m plot) to evaluate cacao clones for resistance and tolerance against Meloidogyne incognita in infested field. The ten cocoa clones evaluated are MXC67, T86/2, PA150, LCTEEN, T12/11, T53/5, T101/15, T65/7, ICS1 and AMAZ 15-15. Pre-planting survey revealed ten genera of phytonematodes, these were; Meloidogyne spp., Pratylenchus spp., Helicotylenchus spp. Paralongidorus spp., Eutylenchus spp., Scutellonema spp., Hemicyclophora spp., Xiphinema spp., Longidorus spp., Anguillulina spp., Psilenchus spp., and Tetylenchus spp. Meloidogyne spp. had the highest frequency of occurrence and highest population in 250g soil (40 and 28,234 respectively), Paralongidorus spp. was next in population. Based on gall index, nematode reproduction factor and growth parameters, MXC67, T86/2, PA150, T101/15 and T53/5 were susceptible to the nematode. Two other clones, T65/7 and ICS1 were tolerant. A high degree of resistance was exhibited by LCTEEN, T12/11 and AMAZ 15-15. Compared with F3 Amazon and Amelonado varieties, the two most famous cocoa varieties in Nigeria, four clones (LCTEEN, T65/7, ICSI and AMAZ 15-15) were superior to F3 Amazon and Amelonado. This study showed that planting of resistance/tolerant cacao clones in nematode infested soil will drastically reduce seedling failure experienced by farmers. This tolerant and resistant clones identified should be included in breeding programme for resistance.
In vitro regeneration of multiple shoots from encapsulated somatic embryos of...IOSR Journals
A protocol has been established for plant regeneration from encapsulated somatic embryos of Artemisia vulgaris L. Encapsulated somatic embryo of A. vulgaris gave better response at 2% alginate, 75mM calcium chloride hardened for 30 minutes. Morphogenic response and percentage of conversion into plantlets of encapsulated beads on storage and temperature effect were studied. Further, encapsulated somatic embryo retrieved from storage conditions was evaluated for its viability. The encapsulated embryos can be stored upto 4 months at 40C, 200C and 220C. Maximum conversion frequency of 90% was observed from encapsulated somatic embryos cultured on MS medium supplemented with GA3 (1.5mg/l), IAA (0.5mg/l) and Ascorbic acid (40mg/l) and 22oC temperature was found to be optimum irrespective of storage periods. In the present investigation, the development of multiple shoots from a single synthetic seed is a recent record.
The experiment was conducted at the experimental farm and laboratory of Institute of
Sustainable Agrotechnology, University Malaysia Perlis, Padang Besar, Perlis, Malaysia, with the objective
toinvestigate the inhibitory and stimulatory effects of aqueous extract of mungbean on seed germination and
seedling growth of three crop species, mungbean, sweet corn and okra. Different treatments of mungbean
aqueousextracts (vegetative fresh, vegetative after 2 weeks drying, vegetative after 4 weeks drying, flowering
fresh, flowering after 2 weeks drying, flowering after 4 weeks drying, flowering fresh, flowering after 2 weeks
drying, flowering after 4 weeks drying, maturity fresh, maturity after 2 weeks drying, maturity after 4 weeks
drying and water as control) were used to test their effect on the test species. The experiment was randomly
distributed and according to Completely Randomized Design(CRD) with five replicates. The results showed the
fresh vegetative aqueous extract of mungbean had a significant effect (stimulatory) on germination percent and
growth parameters such as number of root, root length and shoot height, of the three crop species. The study
revealed that the aqueous extract of mungbean have different effects (inhibitory and stimulatory) on the
seedlings and the mode of action depends on the associated plant species.Our results suggest that the aqueous
extract of mungbean from the different growth stages and drying periods have an allelopathic effect.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
Phosphate solubilizing potential of Rhizobium and Bacillus species for enhanc...Innspub Net
By International Network For Natural Sciences INNSPUB in Agronomy and Horticulture.
A field experiment was conducted to evaluate the separate and integrated effect of Rhizobium and Bacillus spp. on the growth of maize (Zea Mays L.). Inocula of Rhizobium and Bacillus were applied as seed coating. Recommended dose of fertilizer (120-60 kg NP ha-1) was applied at sowing. The treatments were implied according to Randomized Complete Block Design with three repeats. Inoculation had no significant effect on the leaf length (84cm) and internodal distance (18.3cm) compared to their respective control (80cm and16.5cm) but the photosynthetic rate (105.3μ mol-2s-1) , transpiration rate (13.2 mmolm-2s-1), plant height (259.3cm), leaf width (7.7cm), stem diameter (15.43mm), leaf area (644cm-2) and shoot fresh weight (79.6 tones ha-1) were significantly improved by co-inoculation. Effect of Bacillus was statistically at par with co-inoculation regarding transpiration rate (11.47 m mol m-2s-1), plant height (249.3 cm) and stem diameter (14.87 mm). Response of leaf width, stem diameter, leaf area and shoot fresh weight were significantly higher by Rhizobium application compared to the Bacillus inoculation, however, positive influence was observed by all the inoculation treatments over the control.These findings indicated that inoculation of Rhizobium and Bacillus has positive effect on the maize growth and their co-inoculation (Rhizobium+ Bacillus) showed more pronounced results. Get the full articles at: http://www.innspub.net/volume-4-number-1-january-2014-2/
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induc...Agriculture Journal IJOEAR
Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
The use of biotechnology in the propagation of plantain and
banana (Musa sp.) of great importance to induce, tolerant to plant genotypes for
diseases and high yield potentials. However, auxins and cytokinins should be used,
which are expensive and can sometimes cause changes in the regenerants obtained.
Both traditional growth regulators (auxins and cytokinins) and non-traditional growth
regulators (brassinosteroid analogues and mixtures oligogalacturonide) are used in
the in vitro propagation of crops, but mush progress has been hindering due to the
sufficient knowledge and impact of different phases prevailing in the
micropropagation of banana hybrid 'FHIA-18' (AAAB) is present hitherto. This work
was performed in order to evaluate the biological activity of an analogue of
brassinosteroids (Biobras-6) *ABr+ and a mixture of oligogalacturonide with the degree
of polymerization between 9 and 16 (Pectimorf) *mOLG+. The effect of ABr and mOLG
are determined as a substitute or complement of auxin (IBA or IAA) and cytokinin (6-
BAP) for the establishment of in vitro multiplication and rooting of plantlets and in the
acclimatization phase. Non-traditional regulators phenolization decrease the explant
growth in the establishment phase of in vitro propagation; but increased the number
of shoots per explants (above 3.5) and improved survival of vitro plant during the
acclimatization phase.
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
Effect of Different Sources of Nutrient on Growth and Yield of Okra (Abelmosc...Agriculture Journal IJOEAR
The experiment was carried out at Nepal Polytechnic Institute field, Bharatpur, Chitwan, Nepal to study the effect of different nutrient sources on growth and yield of okra (Abelmoschus esculentus L Monech). Five different treatments; poultry manure, FYM, goat manure, chemical (as per N equivalent) and no fertilizer (control) were replicated four times. The experiment was arranged in Randomize Complete Block Design (RCBD). The okra variety ArkaAnamika was used for experiment. The data were collected on the growth and yield parameters including plant height (cm), canopy (cm), numbers of leaves per plant, numbers of branches per plant, fruit length, diameter and yield. Results indicated that different nutrient sources had significant (P<0.05) affected on plant height, canopy, leaf number, branches and also in yield parameters. Based on the findings of the experiments, it can be concluded that application of poultry manure significantly increased the growth and yield performances on Abelmoschus esculentus L. Monech (okra) compared to other types of fertilizers. As the study reflected the use of no fertilizer results in the lowest vegetative growth and yield performances which indicates to use some nutrient sources for better growth and production of okra.
Efficacy of rice-stubble allelochemicals on vegetative growth parameters of s...ijtsrd
A laboratory experiment was conducted to investigate allelopathic potential of water extract of rice-stubble leachate on certain oil-yielding crops mustard, sesamum and sunflower . A wide range of rice-stubble concentration i.e. 5, 10, 15, 20, 25, 30 and 40 were prepared and used for testing of seedling growth parameters Length, fresh and dry weight of shoot and root of oil-yielding test crop seedlings. The experimental results found that the different seedling growth parameters were reduced with increased concentration of rice-stubble leachate in compared to control set. Root growth was more sensitive than shoot growth with increase of leachate concentration of rice-stubble. This experimental results show the existence of several bioactive compounds i.e. allelochemicals in rice-stubble. These bioactive compounds might serve as a source of natural herbicides. S. P. Adhikary "Efficacy of rice-stubble allelochemicals on vegetative growth parameters of some oil-yielding crops" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd20251.pdf
Paper URL: https://www.ijtsrd.com/biological-science/botany/20251/efficacy-of-rice-stubble-allelochemicals-on-vegetative-growth-parameters-of-some-oil-yielding-crops/s-p-adhikary
Application of arbuscular mycorrhizal fungi accelerates the growth of shoot r...UniversitasGadjahMada
Shoot roots are second type of root, which emerge from the base of the new shoots, 5-7 days after planting. The shoot roots growth on single bud chips seedling is critical for further growth in dry land. The objectives of this study were to examine shoot root growth using different doses of arbuscular mycorrhizal fungi (AMF) inoculum on five clones of sugarcane and to ascertain their effect on seedling biomass weight. The highest and lowest temperatures on the research site were 32º and 18 ºC, in tropical monsoon climate. The experimental design was a completely randomized design (CRD) in 4x5 factorial arrangement with four replicates. The treatments were: four doses of AMF inoculum (0, 1, 2, 3 g/bud chips) on five clones with single bud chips seedling (PS864, KK, PS881, BL, and VMC). The evaluated parameters were root colonization affected by doses of AMF inoculum, number of shoot roots, surface area of shoot and total roots, root length, biomass seedling, and P leaf concentration affected by doses of AMF inoculum. AMF inoculum doses of 2 and 3 g of inoculum/bud chips resulted in the speed and extent root colonization at 5 days after inoculation on all five sugarcane clones. The clones exhibited 57-100 % accelerated emergence of shoot roots (i.e. the second roots formed), increased total root length, total root surface area especially on BL, VMC, and P leaf concentration. Application of 2-3 inoculum/bud of AMF inoculum significantly increased shoot roots growth i.e. root length, root surface area, and number of shoot roots.
Micropropagation of Santalum Album L. Sandalwoodijtsrd
An efficient plant regeneration protocol was developed for Santalum album L. Santalaceae , an economically important species. Plant regeneration was achieved using nodal explants and leaf disc on Murashige and Skoog MS medium for direct shoot regeneration. Effect of Plant Growth Regulators PGR like 6 Benzyl Adenine BA , Kinetin KN and 2 Isopentenyl adenine 2 iP on shoot initiation 2 Isopentenyl adenine and Gibberellic acid GA3 for shoot elongation and multiple shoot formation and Indole 3 Butyric Acid IBA and a Naphthalene Acetic Acid NAA for rooting was studied. Among the explants tested for shoot induction, nodal segments proved good results. The best treatment for obtaining shoot induction was 3.0mg L BAP and for rooting 1 mg L of IBA was found to be the best treatment combination for maximum sprouting of shoot and rooting. After six the rooted plantlets were transferred for hardening, 20 of plantlets survived and resumed growth in the mixture of soil, vermiculite and sand 1 1 1 . S. Aghi Zion Inbakani | S. Sathishkumar | Bakan Jagdish Sudhakar "Micropropagation of Santalum Album L. (Sandalwood)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43698.pdf Paper URL: https://www.ijtsrd.combiological-science/biotechnology/43698/micropropagation-of-santalum-album-l-sandalwood/s-aghi-zion-inbakani
Mutation Induction for Improvement of Banana (Musa Spp). "Berangan Cv. Intan-...paperpublications3
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Mutation Induction for Improvement of Banana (Musa Spp.) Berangan Cv. Intan-AAApaperpublications3
Abstract: In vitro mutation induction by using gamma ray at 20, 30, 40 and 60Gy was used to generate variability in triploid banana "Berangan cv. Intan (AAA), so as to provide the opportunity to select plants with desirable characters such as early fruiting and short stature. Mutation frequency increased with increased dosage whereas, survival and capacity to regenerate decreased with increased doses. Time to initiation varied from 4-8 weeks for gamma-irradiated materials compared to 2-3 weeks in the control. It appeared that the higher the dose, the longer it took for shoot initiation. The exposure of shoot-tip meristem pieces to radiation doses produced wide variation in growth and morphogenetic performance. Mutagenic treatments induced 2 to 3- fold increases in variability in both quantitative and qualitative traits at different stages, in vitro; at nursery and field. For the field-grown plants; the proportion varied from 2.9% for the control plants to 16.8% for 60 Gy and 20.1% for 40 Gy-treatments, while for treatments at 20 and 30 Gy variations was at 18% and 19.2%, respectively. The frequency of variants was highest in 40 Gy followed by 30 Gy and 20 Gy, while it was very low in 60 Gy except for plant stature (dwarfism or stunted growth). Earliness to flowering variants were recorded at 20, 30 and 40 Gy at low frequencies (0.6%, 0.7% and 1.7%) respectively, while none was observed for 60 Gy treatments. As in vitro mutation induction could create genetic variability as well as many undesirable variants, it is highly desirable to integrate in vitro mutation with a selection system that can screen for large mutagen treated population. The useful variants recorded for earliness to flowering were selected for 30 and 40 Gy treatments. 40 Gy showed high frequency in earliness as compared with 20 and 30 Gy., which came to flowering as early as 6 – 6.5 months compared to 7-8 months for control. The useful dwarf, which considered as desirable traits, showed a balance of height and girth.
Criteria for the Selection of Vegetable Growth-Promoting Bacteria to be appli...Agriculture Journal IJOEAR
In order to define which are the most important criteria for the selection of plant Growth-Promoting bacterial strains of the Hibiscus sabdariffa L. crop (Roselle), bacterial strains isolated from the roots of Roselle plants of two varieties (Creole and Spider) were used, collected in the community of Río de los Peces, municipality of Candelaria Loxicha, Oaxaca and seeds of the same varieties. To characterize the varieties, the following were determined: total germination percentage (TGP), germination speed (GS), the root length(RL), the stem length (SL), the dry root biomass (DRB), the dry stem biomass (DSB) and the chlorophyll content (CC). Three types of LED lamps were used to illuminate the seedlings. The seeds inoculated with cells of six selected bacterial strains were grown in a greenhouse to determine: the stem length (SL) at 3, 45 and 65 days after sowing (das). The treatments were distributed under a completely random design and comparison of means (Tukey, p = 0.05). The TGP, DSB and DRB parameters were not useful in the selection process of the strains that promoted plant growth to a greater degree. The GS and SL to be considered safe criteria or not, what is important is the relationship of what happens at the time of germination and development of the seedlings in the laboratory and greenhouse. The SL of the plants in the greenhouse showed differences between strains, but not regarding the control and also only observed in the first days of development (3 das). The CC did not prove to be a good selection criterion either. The lamp composed of 15% white light, 27% blue light and 58% red light was the one that most promoted root growth.
Similar to Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated Sesame (Sesamum indicum L (20)
Diabetes is a rapidly and serious health problem in Pakistan. This chronic condition is associated with serious long-term complications, including higher risk of heart disease and stroke. Aggressive treatment of hypertension and hyperlipideamia can result in a substantial reduction in cardiovascular events in patients with diabetes 1. Consequently pharmacist-led diabetes cardiovascular risk (DCVR) clinics have been established in both primary and secondary care sites in NHS Lothian during the past five years. An audit of the pharmaceutical care delivery at the clinics was conducted in order to evaluate practice and to standardize the pharmacists’ documentation of outcomes. Pharmaceutical care issues (PCI) and patient details were collected both prospectively and retrospectively from three DCVR clinics. The PCI`s were categorized according to a triangularised system consisting of multiple categories. These were ‘checks’, ‘changes’ (‘change in drug therapy process’ and ‘change in drug therapy’), ‘drug therapy problems’ and ‘quality assurance descriptors’ (‘timer perspective’ and ‘degree of change’). A verified medication assessment tool (MAT) for patients with chronic cardiovascular disease was applied to the patients from one of the clinics. The tool was used to quantify PCI`s and pharmacist actions that were centered on implementing or enforcing clinical guideline standards. A database was developed to be used as an assessment tool and to standardize the documentation of achievement of outcomes. Feedback on the audit of the pharmaceutical care delivery and the database was received from the DCVR clinic pharmacist at a focus group meeting.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated Sesame (Sesamum indicum L
1. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-3, Issue-3, March- 2017]
Page | 30
Comparison of Resistance to Fusarium wilts disease in Seeded
and Regenerated Sesame (Sesamum indicum L.)
Firoozeh Chamandoosti
Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO),
Tehran, Iran
PhD of Cellular and Developmental Biology, Assistant Professor of Iranian Research Institute of Plant Protection
Department of Plant Diseases
Abstract— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal
variation that occurred in many different crops. In this study a program for disease resistance was established in sesame
using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth
Regulators were tested for producing of plantlet regenerated from apical shoot explants.
The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame
plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at
least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant
and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were
compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from
regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Keywords— fusarium, sesame, somaclonal variation.
Abbreviations: ABA – Abscisic acid; BA – 6 benzylaminopurin; Fos – Fusarium oxysporum fsp sesami, IAA – Indole-3
acetic acid; MS – Murashige and Skoog,
s medium 1962; NAA – α-naphthalene acetic acid; PGRs – Plant Growth
Regulators.
I. INTRODUCTION
Somaclonal variation, resulting from a sum of genetic and epigenetic changes can induce mutations (Wei et al 2016).
Somaclonal variation occurs through tissue culture in plants and plant tissue culture techniques proffer a substitute method of
vegetative propagation of horticultural crops (Krishna et al. 2005; Alizadeh et al. 2010). On the other hand somaclonal
variation is a basic method for inducing resistance in many plants against biotic and abiotic stresses (Chae et al., 1987; Kim
et al., 1987 and Kariallappa, 2003). However Larkin and Scowkraft in 1981 coined a general term “somaclonal variation” for
plant variants derived from any form of cell or tissue cultures, It is important that genetic variations occur in undifferentiated
cells, isolated protoplasts, calli, tissues and morphological traits of in vitro raised plants (Bairu et al. 2011; Currais et
al. 2013). So for inducing of variation in plants duo to somaclonal variation at first we need to a suitable and practical system
for regeneration of plants. It means that denovo organs must to be regenerated by callusing phase. In this research we
established a system for sesame regeneration and compared resistance of regenerated and seeded sesame against Fusarium
oxysporum fsp sesami that is a one of the most devastating agents for sesame in Iran. Also In Iran alike all over the world
sesame is the quine of oilseed.
This important oilseed ranks third among the oilseed in production. Its oil content varies from 44 – 66 % containing two
unsaturated fatty acids – oleic and linoleic together account for 85% (Maximum) with a combination of different essential
amino acids and vitamins particularly β carotene (Brar, 1982 and Arslan et al., 2007).
II. MATERIALS AND METHODS
2.1 Preparation of plant materials
Seeds of sesame CV. Darab1 were obtained from Seed and Plant Improvement Institute, Karaj, Iran.
Mature seeds of sasame were aseptically sterilized by immersing in 100% commercial hypochlorite sodium (with 5%
available chlorine) for 20, 30 and 60 min. Sterilized seeds then rinsed with sterilized distilled water for 4 – 5 times in order to
remove the effects of disinfecting agent then cultured on 0.5 MS (Murashige and Skoog 1962), plus 3% sucrose and 0.7%
agar in 9 cm petri dishes that were closed with parafilm. The cultures maintained in room temperature and mild light. Seven
2. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-3, Issue-3, March- 2017]
Page | 31
days later pieces of hypocotyl (3 – 5 mm), apical shoots ( 2 – 3 mm) and cotyledon (9 – 16 mm2
) from seedlings were used as
explants.
2.2 Media and culture conditions
For production of regenerated plants the explants were cultured on MS media supplemented with different kinds and
concentrations of PGRs such as BA, IAA, NAA, ABA and 554.94 µm-1
myo-Inositol, 0.3 µml-1
Thiamin, 24.3 µml-1
Pyridoxine, 4.06 µml-1
Nicotinic acid , and 26.64 µml-1
Glycine. Explants were subcultured to fresh media with the same
composition every 4 weeks duo to consumption of nutrition and oxygene. All media included 3% sucrose and 4% phytagel in
this phase.
pH of all media was adjusted to 5.7 prior to autoclaving. All cultures were incubated at 25 ± 2 º C under a 16-h photoperiod
and 1500 Lux illumination.
2.3 Inoculum preparation
To prepare of inoculum approximately 3 – 4 discs (1 cm2
length) from PDA including Fos mycelia were cut and put on
autoclaved wheat grains that placed in clear plastic bag. Clear plastic bag plugged by cotton and incubated at 25º for three
weeks. Seventy five grams inoculum was mixed with two kg autoclaved soil and prepared for each pot before sowing.
Plantlets inoculation and evaluation of resistance
Two kinds of plantlets including seeded plantlets (4 foliage) and regenerated plantlets (approximately 4 foliage) planted in
inoculated soil for evaluating for resistance or susceptibility against Fos. For evaluation of resistance dead or alive of
plantlets was mentioned (See Pavlou)
III. RESULTS AND DISSCUTION
Explants (Leaf, hypocotyl and apical shoot) produced callus after nearly two weeks. Leaf explants doubled in size and
produced callus on the wounded edges. Hypocotyl explants produced callus on their entire surface and apical shoots
produced callus a little. The calli were apparently categorised based on the potential of organogenesis or embryogenesis. The
effective factore on this classification was at first the component of media and the second explant type. So that on media
supplemented with BA the adventitious shoots developed from hypocotyl and apical shoot within 14 – 28 days after
callusing. Shoot developed much more by increasing of BA concentration. 4.44 and 22 µml-1
BA produced adventitious
shoots with 5.70 µml-1
IAA + 3.78 µml-
1 ABA and 1.61 µml-1
NAA respectively (Fig 1). Because a cluster of shoots usually
formed on explants it was difficult to count the number of shoots on these two media. It is clear that regeneration efficiency
in a range of BA ( 4.44 – 22 µml-1
) increased as BA concentrations increased but dense mass of shoots formed on explants
affected not only by BA concentrations but also kind and concentration of auxins and other plant growth regulators. On the
other hand BA concentrations with auxin(s) concentrations had influence in shoot regenerations. In Fig 7 the effect of BA
and auxin(s) (NAA) on shoot regeneration was summerized. Also this picture shows that the kind of auxin(s) that cooperats
with BA on shoot regeneration has an effective role. Shoots (1 – 2 cm) were excised and cultured on suitable rooting media
especially medium with 8.05 µml-1
NAA and 0.13 µml-1
BA. After 7 - 10 days 5 – 6 white, thick and semi strong roots
appeared on base of regenerated shoots (Fig 2). It is interesting that BA and NAA with different balances formed both shoot
and root in sesame. Successful regeneration have a key role for somaclonal variation. At BA and NAA concentrations
regeneration tended to shooting by rising in BA concentration and rooting by rinsing in NAA concentration. Similar results
were observed in apple cultivars or rootstocks (Welanders, 1992; Ancherani et al., 1990; Yepes et al., 1994 and Famiani et
al., 1994) where the low concentration of auxin in combination with height cytokinin content resulted in an increase in
number of shoots per explants. Also there are many reports about the positive effect of BA in shooting (Lee et al., 2003;
Ahmad et al., 2010; Rai et al., 2012; Kadota et al., 2001).
After establishment of regenerated plantlets and seeded plantlets the resistance (Fig 3 and Fig 4 respectively) of both
compared against Fos. Two different kinds of plantlets displayed significant differences. According to Pavlou and
Vakalounakis in 2005 sign of necrosis in xylem and phloem, dead or dying plantlets and wilt is indicator for susceptibility.
As figures show four foliage seeded planlets after transplanting in inoculated soil displayed all of mentioned signs and dead
after 20 days completely (Fig 5). So regenerated plantlets not only died but also grew and produced new leaflets after 12 – 20
days transplanting in inoculated soil (Fig 6). So we can result resistance to Fos altered in sesame by tissue culture. It is clear
that a wide range of plant characteristics can be altered as a result of regeneration from cell and tissue culture including
agronomacally important traits such as diseases resistance (Van den bulk 1991 and Sebastiani et al., 1994). Also Somaclonal
3. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-3, Issue-3, March- 2017]
Page | 32
variation was claimed to be a source of variation for crop improvement (Ching – Yan Tang, 2005). Although somaclonal
variation has been reported in crop with reproduction vegetatively (Larkin and Scowcroft, 1981) more there are a few reports
about selection of somaclonal resistant to alternaria blight in sesame through tissue culture (Lokesha and Naik, 2011). On the
other hand Fusarium is one of the most devastating microorganism that has been combatted by somaclonal variation (Pierk,
1994 and Ching Yan – Tang, 2005). Also this study that their results demonstrated the resistance of regenerated sesame
plantlels against Fos agreed with researches that mentioned above. As figures show clearly one of the difficult in this
research was weakness and smallness of regenerated plants when they compared with seeded plantlets duo to unfavourable
conditions especially oxygen deficiency and high level of humidity. We tested seeded plantlets in 4 folige stage. Long-term
maintenance (regenerated plantlets) in order to complete adaptation was not our purpose. But it is interesting that regenerated
plantlets resisted against pathogen (Fos) despite of weakness and smallness.
So included in this study that would be presented method for inducing resistance in sesame by somaclonal variation as the
one of results of tissue culture.
4. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-3, Issue-3, March- 2017]
Page | 33
FIGURE 1 – 6. Occurance of somaclonal variation in regenerated sesame and the effect of it on resistance or
susceptibility plantlets to Fos. 1- Shooting on apical shoots explants on MS medium with 8.88 µml-1
BA and 1.61 8.88
NAA. Rooting of regenerated shoots on MS medium with 8.05µml-1
NAA and 0.13 µml-1
BA. 3- Regenerated plantlets
in pot. 4- Seeded plantlets in 4 foliage phase. 5-Dead seeded plantlets 20 days after inoculating with Fos. 6- Resistance
of regenerated plantlets 20 days after inoculating with Fos.
FIGURE 7. Effect of elavated BA concentrations with 5.7 mµl-1
IAA + 3.78 mµl-1
ABA (T1) and 1.61 mµl-1
NAA (T2)
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