Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Synergetic effects of various plant extracts as bio-pesticide against wheat a...Muhammad Qasim
Human health and environment are greatly affected by extensive used of synthetic insecticide, which is why the alternative way of pesticides, such as botanical pesticide consumption increase with the passage of time to control of insect pests. The insecticidal impact of Moringa (Moringa oleifera), Neem (Azadirachta indica) and Euclyptus (Eucalyptus globules) leaves extracts were investigated on Diurophous noxia (Hemiptera: Aphididae). This study was aimed to evaluate the efficiency of bio-pesticide instead of synthetic chemicals against D. noxia. Experimental results showed that the percentage of mortality fluctuate with the fluctuation of concentration with time intervals and have great impact on the population of D. noxia. Single, doubled and tripled combinations of leaves extract were tested at two different concentrations (25 and 50%). The results suggested and concluded that from all the application of single botanicals A. indica give better result as compared to other single application while application of double combination M. olerifera with A. indica give significance result instead of other double mixture, meanwhile the triple combination (M. olerifera, A. indica and E. globules) performed excellent result as the others triple mixtures. The study revealed that combine used of M. olerifera, A. indica and E. globules leaves extract were very effective against D. noxia and surge as an alternative way of pesticides instead of synthetic chemicals.
Role of new generation plant bioregulators in fruitSindhu Reddy
In order meet out the emerging consumer demand and challenges towards fruit production, there is the need to explore new interventions. One among that is use of new generation plant growth regulators in fruit crops. Plant growth regulators (PGR), recently name has been changed to plant bio-regulators (PBR’s) are defined as organic compounds, other than nutrients, that in small concentrations, affect the physiological processes of plants. There are five classical growth hormones which have the specific function in growth and development were already commercially exploited in fruit crops, but use of new generation growth regulators in fruit crops are recent and emerging trend. New generation PBR’s includes brassinosteroids, Jasmonate, salicylic acid, polyamines, karrikins and strigolactones and retardants such as 1-MCP and prohexodione-Ca. These are utilized in fruit crops starting from propagation to improving quality also including biotic and abiotic stress resistant. Hence, new generation plant growth regulators are an effective alternative for future fruit production combating major production challenges.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
Effect of Different Concentrations of Glycerol on survival of Azotobacter chr...IIJSRJournal
The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 25 mM in the medium containing glycerol (38.66 colonies on zero day and 22 colonies on 360th day) (109 CFU/ml).
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
Synergetic effects of various plant extracts as bio-pesticide against wheat a...Muhammad Qasim
Human health and environment are greatly affected by extensive used of synthetic insecticide, which is why the alternative way of pesticides, such as botanical pesticide consumption increase with the passage of time to control of insect pests. The insecticidal impact of Moringa (Moringa oleifera), Neem (Azadirachta indica) and Euclyptus (Eucalyptus globules) leaves extracts were investigated on Diurophous noxia (Hemiptera: Aphididae). This study was aimed to evaluate the efficiency of bio-pesticide instead of synthetic chemicals against D. noxia. Experimental results showed that the percentage of mortality fluctuate with the fluctuation of concentration with time intervals and have great impact on the population of D. noxia. Single, doubled and tripled combinations of leaves extract were tested at two different concentrations (25 and 50%). The results suggested and concluded that from all the application of single botanicals A. indica give better result as compared to other single application while application of double combination M. olerifera with A. indica give significance result instead of other double mixture, meanwhile the triple combination (M. olerifera, A. indica and E. globules) performed excellent result as the others triple mixtures. The study revealed that combine used of M. olerifera, A. indica and E. globules leaves extract were very effective against D. noxia and surge as an alternative way of pesticides instead of synthetic chemicals.
Role of new generation plant bioregulators in fruitSindhu Reddy
In order meet out the emerging consumer demand and challenges towards fruit production, there is the need to explore new interventions. One among that is use of new generation plant growth regulators in fruit crops. Plant growth regulators (PGR), recently name has been changed to plant bio-regulators (PBR’s) are defined as organic compounds, other than nutrients, that in small concentrations, affect the physiological processes of plants. There are five classical growth hormones which have the specific function in growth and development were already commercially exploited in fruit crops, but use of new generation growth regulators in fruit crops are recent and emerging trend. New generation PBR’s includes brassinosteroids, Jasmonate, salicylic acid, polyamines, karrikins and strigolactones and retardants such as 1-MCP and prohexodione-Ca. These are utilized in fruit crops starting from propagation to improving quality also including biotic and abiotic stress resistant. Hence, new generation plant growth regulators are an effective alternative for future fruit production combating major production challenges.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
Effect of Different Concentrations of Glycerol on survival of Azotobacter chr...IIJSRJournal
The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 25 mM in the medium containing glycerol (38.66 colonies on zero day and 22 colonies on 360th day) (109 CFU/ml).
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
In vitro callus induction of Melothria purpusilla, a traditional medicinal pl...IJERA Editor
Melothria purpusilla, a member of Cucurbitaceae, is an endemic species found in North-Eastern part of India.
The plant is used traditionally by the people of Manipur in the treatment of jaundice and its roots in fever and
diarrhoea. Tissue culture of medicinal plants was performed as a measure for the conservation of endangered
medicinal plants, Melothria perpusilla. Morphogenetic changes were observed in Melothria perpusilla
explants in the MS medium supplemented with different concentrations of PGRs. Different colours of callus
formation were observed in MS supplemented with BAP, kinetin and IBA. The best callus induction was
observed with MS media supplemented with combination of 1BAP mg/l + 1 IBA mg/l and combination of
1Kinetin mg/l + 1 IBA mg/l.
DOI:10.21276/ijlssr.2016.2.4.5
ABSTRACT- Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were
the world’s largest producer and exporter of cardamom till 1980. There has however been a reduction in production,
mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed
effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from
Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised
against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA
(DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid
in using disease free material for better yields and hence increased profit to the farmer. Key-words- Cardamom mosaic virus (CdMV), Electron microscopy, Direct antigen coating Enzyme linked
immunosorbent Assay (DAC-ELISA), Dot-ELISA (DIBA)
Germination Capacity in Culture Medium of Prosopis Laevigata Seeds in the Pre...Agriculture Journal IJOEAR
Copper is a heavy metal that has been used as an anti-fungal agent in various crops, this is why it accumulates in certain agricultural lands at levels that become toxic to plants, as well as to microflora. Cooper, although essential to plants, is toxic when found in high concentrations. The objective of this study was to determine if this element is capable of stimulating and at the same time inhibiting germination of seeds of Prosopislaevigata (mesquite) depending on concentration of CuS0 4 .5H 2 0 solutions. A completely randomized experimental design with seven treatments and three repetitions was used to determine tolerance of mesquite seeds to copper. The variables evaluated were percentage of daily germination (PDG-A, PDG-B, and PDG-C), accumulative germination (AG-A, AG-B, and AG-C), average germination time (AGT), germination rate (GR) and anhydrous weight (AW) of mesquite seeds. The culture media supplied with concentration of 10-4 M of copper sulfate (CuS0 4 .5H 2 0), corresponding to treatment four (T 4) showed significant differences (p≤0.05) in variable percentage of daily germination at 48 hours (PDG-B), which presented a germination of 66.7% in relation to treatment two (T 2) with a concentration of 10-2 M of CuS0 4 .5H 2 0 and germination of 22.2%. Results obtained after 72 hours for percentage daily germination variable (PDG-C) with > p <0.1 showed that mesquite is a species that can tolerate and adapt in germination stage for culture medium with concentrations from 10-2 to 10-7 M of CuS0 4 .5H 2 0, and consequently use of seedlings for phytoremediation of sites contaminated with copper.
Study of Bioactive Components Present in Oil Extracted from Fenugreek Seed: R...AI Publications
A fenugreek seed (Trigonella foenum-graecum) is a native of India, and also found in Mediterranean region, southern Europe, Africa and western Asia. These seeds have their importance due to the medicinal properties as well as nutraceutical value present in their chemical composition. This fenugreek seeds were used for treating diabetes, menstrual cramps, cancer, heart related disease, skin and hair fall treatments, high per tension, weight loss, to increase milk supply in women who were breastfeeding. This seeds is found to be abundant in N-Dimethylaminomethyl, 1-ptoprnol, 2, 2-dimethyl-aceto, Aziridine,1,2,3-trimethyl-trans, 2-pentanone,4-hydroxy-4 meth, 2-trimethylsily-1,3-dithiane, 3-0-methyl-d-glucose, d-mannopyranoiside,uronate,Diazidine1,3,3-trimethyl,Methyl 4,8dimethylnonanoate There are numerous methods available on extraction of these compounds right from conventional (Maceration, Percolation, Reflux extraction, Soxhlet extraction, microwave assist extraction, pressurised liquid extraction, super critical fluid extraction, Pulsed electric field extraction, Enzyme assisted extraction, Ultrasound assisted extraction) apart from this some water based extraction technique may also use to extract bioactive compounds from fenugreek seeds. In present article a focus has been given to highlight importance of fenugreek seeds extract in terms of extraction of bioactive compounds and their probable application in the area of food and pharmaceuticals.
Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated ...Agriculture Journal IJOEAR
— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal variation that occurred in many different crops. In this study a program for disease resistance was established in sesame using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth Regulators were tested for producing of plantlet regenerated from apical shoot explants. The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Allelopathic Effect of Lantana Camara on Germinatiion and Growth of Chickpea ...IJAEMSJORNAL
An experiment was conducted in Department of Botany, BFIT, Dehradun, Uttrakhand during the months of February and March to understand the allelopathic effects of different concentrations taken through the leaf powder of Lantana camaraleaf on germination percentage and early growth of two pulse crops i.e. chickpea and green gram. The test was conducted in petridishes. Results showed that different leaf powder concentration cause great inhibitory effect on germination and growth of both the pulse crops. Results indicates that the allelochemicals were released from the leaf powder and inhabit the germination and growth of germinated crops.
Drought stress is one of the major abiotic factors affecting seed germination and plant growth especially in arid and semi-arid regions. In this study, we investigated the effects of drought stress on seed germination and seedling growth of five varieties of rapeseed. Seven drought stress levels of zero (control), -3, -5, -7, -9, -11 and -13 bars were performed using polyethylene glycol-6000 (PEG-6000). A completely randomized design with three replications was used for this experiment. Germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL) and shoot length (SL) were measured to evaluate the varieties response to PEG-induced drought stress. Drought stress, variety and the interaction drought × variety had a significant effect on all studied parameters. GP and GR decreased with the increase in stress level, while MGT increased. There were no seeds germinated for all varieties at -11 bars and -13 bars. Shoot length decreased with increasing drought stress but different varieties show different performance under stress environment. Root length decreased with increasing level of severe drought stress. However, the presence of moderate drought stress could even improve the root growth of the investigated varieties. The varieties ‘INRA-CZH2’ and ‘INRA-CZH3’ exhibited the highest germination percentage and the best early seedling growth. Thus, they could be recommended for environments with early cropping cycle drought.
in vitro screening of larvicidal and insecticidal activity of methanolic extr...IJEAB
The aim of this work was to evaluate the larvicidal and insecticidal activity of the selected plants namelyArtocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction for the isolation of various bioactive constituents. The evaluation of larvicidal activity was carried out using late third instar larvae of Drosophila melanogaster. The insecticidal activity of extracts was studied against adult Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae and was evaluated by direct contact application method .Nucleic acids and protein contents are regarded as important biomarkers of the metabolic potential of cells, as these play the main role in regulating the different activities of cells. Piper betle and Artocarpus heterophyllusextractshad a reducing effect on the nucleic acid and protein content in the larvae in a dose dependent manner whereas Artocarpus altilis extract did not exhibit any significant larvicidal activity. Piper betle and Artocarpus heterophyllusextractsshowed good insecticidal activity whereas A.altilis extract showed poor insecticidal activity. The results of the present study clearly indicate that Piper betle and Artocarpus heterophyllus extracts can be developed as ecofriendly larvicides and were also quite effective as insecticides for providing a better and excellent alternate for the control of insects.
This is the second in a series of presentations for students in the Department of Creative Professions and Digital Arts, University of Greenwich: How to think about social media presence, public and private, content, SEO, visibility, and how to write a good clickbait heading.
Effects of β-cyclodextrin on in vitro rooting and bulbing of lilium (Lilium l...Premier Publishers
The auxins liability often limits expected effects on in vitro culture of plant tissues. In this regard, β-cyclodextrine was used as protective substance to auxins in order to improve rooting and in vitro bulbing of lily (Lilium longiflorum L.). This oligosaccharide was added at a concentration of 10, 20 and 30 mg/l to the rooting medium containing mineral and vitamin additives of Murashige and Skoog (MS), 1.5 mg/l of indole-3-butyric acid (IBA), 30 g/l of sucrose and 6 g/l of agar. After 30 days of culture, in order to enhance in vitro bulbe formation, a liquid medium supplemented with 6-benzylaminopuine (BAP) was added to form a double phase (solid / liquid) media. The results obtained showed that β-cyclodextrin improves the rooting of lily in vitro shoots. Indeed, the average number of roots increases from 5.64 to 7.20 roots per vitro-plant in the medium supplemented with β-cyclodextrin and root length increased from 1.72 to 2.2 cm compared to control.
In vitro callus induction of Melothria purpusilla, a traditional medicinal pl...IJERA Editor
Melothria purpusilla, a member of Cucurbitaceae, is an endemic species found in North-Eastern part of India.
The plant is used traditionally by the people of Manipur in the treatment of jaundice and its roots in fever and
diarrhoea. Tissue culture of medicinal plants was performed as a measure for the conservation of endangered
medicinal plants, Melothria perpusilla. Morphogenetic changes were observed in Melothria perpusilla
explants in the MS medium supplemented with different concentrations of PGRs. Different colours of callus
formation were observed in MS supplemented with BAP, kinetin and IBA. The best callus induction was
observed with MS media supplemented with combination of 1BAP mg/l + 1 IBA mg/l and combination of
1Kinetin mg/l + 1 IBA mg/l.
DOI:10.21276/ijlssr.2016.2.4.5
ABSTRACT- Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were
the world’s largest producer and exporter of cardamom till 1980. There has however been a reduction in production,
mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed
effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from
Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised
against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA
(DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid
in using disease free material for better yields and hence increased profit to the farmer. Key-words- Cardamom mosaic virus (CdMV), Electron microscopy, Direct antigen coating Enzyme linked
immunosorbent Assay (DAC-ELISA), Dot-ELISA (DIBA)
Germination Capacity in Culture Medium of Prosopis Laevigata Seeds in the Pre...Agriculture Journal IJOEAR
Copper is a heavy metal that has been used as an anti-fungal agent in various crops, this is why it accumulates in certain agricultural lands at levels that become toxic to plants, as well as to microflora. Cooper, although essential to plants, is toxic when found in high concentrations. The objective of this study was to determine if this element is capable of stimulating and at the same time inhibiting germination of seeds of Prosopislaevigata (mesquite) depending on concentration of CuS0 4 .5H 2 0 solutions. A completely randomized experimental design with seven treatments and three repetitions was used to determine tolerance of mesquite seeds to copper. The variables evaluated were percentage of daily germination (PDG-A, PDG-B, and PDG-C), accumulative germination (AG-A, AG-B, and AG-C), average germination time (AGT), germination rate (GR) and anhydrous weight (AW) of mesquite seeds. The culture media supplied with concentration of 10-4 M of copper sulfate (CuS0 4 .5H 2 0), corresponding to treatment four (T 4) showed significant differences (p≤0.05) in variable percentage of daily germination at 48 hours (PDG-B), which presented a germination of 66.7% in relation to treatment two (T 2) with a concentration of 10-2 M of CuS0 4 .5H 2 0 and germination of 22.2%. Results obtained after 72 hours for percentage daily germination variable (PDG-C) with > p <0.1 showed that mesquite is a species that can tolerate and adapt in germination stage for culture medium with concentrations from 10-2 to 10-7 M of CuS0 4 .5H 2 0, and consequently use of seedlings for phytoremediation of sites contaminated with copper.
Study of Bioactive Components Present in Oil Extracted from Fenugreek Seed: R...AI Publications
A fenugreek seed (Trigonella foenum-graecum) is a native of India, and also found in Mediterranean region, southern Europe, Africa and western Asia. These seeds have their importance due to the medicinal properties as well as nutraceutical value present in their chemical composition. This fenugreek seeds were used for treating diabetes, menstrual cramps, cancer, heart related disease, skin and hair fall treatments, high per tension, weight loss, to increase milk supply in women who were breastfeeding. This seeds is found to be abundant in N-Dimethylaminomethyl, 1-ptoprnol, 2, 2-dimethyl-aceto, Aziridine,1,2,3-trimethyl-trans, 2-pentanone,4-hydroxy-4 meth, 2-trimethylsily-1,3-dithiane, 3-0-methyl-d-glucose, d-mannopyranoiside,uronate,Diazidine1,3,3-trimethyl,Methyl 4,8dimethylnonanoate There are numerous methods available on extraction of these compounds right from conventional (Maceration, Percolation, Reflux extraction, Soxhlet extraction, microwave assist extraction, pressurised liquid extraction, super critical fluid extraction, Pulsed electric field extraction, Enzyme assisted extraction, Ultrasound assisted extraction) apart from this some water based extraction technique may also use to extract bioactive compounds from fenugreek seeds. In present article a focus has been given to highlight importance of fenugreek seeds extract in terms of extraction of bioactive compounds and their probable application in the area of food and pharmaceuticals.
Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated ...Agriculture Journal IJOEAR
— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal variation that occurred in many different crops. In this study a program for disease resistance was established in sesame using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth Regulators were tested for producing of plantlet regenerated from apical shoot explants. The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Allelopathic Effect of Lantana Camara on Germinatiion and Growth of Chickpea ...IJAEMSJORNAL
An experiment was conducted in Department of Botany, BFIT, Dehradun, Uttrakhand during the months of February and March to understand the allelopathic effects of different concentrations taken through the leaf powder of Lantana camaraleaf on germination percentage and early growth of two pulse crops i.e. chickpea and green gram. The test was conducted in petridishes. Results showed that different leaf powder concentration cause great inhibitory effect on germination and growth of both the pulse crops. Results indicates that the allelochemicals were released from the leaf powder and inhabit the germination and growth of germinated crops.
Drought stress is one of the major abiotic factors affecting seed germination and plant growth especially in arid and semi-arid regions. In this study, we investigated the effects of drought stress on seed germination and seedling growth of five varieties of rapeseed. Seven drought stress levels of zero (control), -3, -5, -7, -9, -11 and -13 bars were performed using polyethylene glycol-6000 (PEG-6000). A completely randomized design with three replications was used for this experiment. Germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL) and shoot length (SL) were measured to evaluate the varieties response to PEG-induced drought stress. Drought stress, variety and the interaction drought × variety had a significant effect on all studied parameters. GP and GR decreased with the increase in stress level, while MGT increased. There were no seeds germinated for all varieties at -11 bars and -13 bars. Shoot length decreased with increasing drought stress but different varieties show different performance under stress environment. Root length decreased with increasing level of severe drought stress. However, the presence of moderate drought stress could even improve the root growth of the investigated varieties. The varieties ‘INRA-CZH2’ and ‘INRA-CZH3’ exhibited the highest germination percentage and the best early seedling growth. Thus, they could be recommended for environments with early cropping cycle drought.
in vitro screening of larvicidal and insecticidal activity of methanolic extr...IJEAB
The aim of this work was to evaluate the larvicidal and insecticidal activity of the selected plants namelyArtocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction for the isolation of various bioactive constituents. The evaluation of larvicidal activity was carried out using late third instar larvae of Drosophila melanogaster. The insecticidal activity of extracts was studied against adult Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae and was evaluated by direct contact application method .Nucleic acids and protein contents are regarded as important biomarkers of the metabolic potential of cells, as these play the main role in regulating the different activities of cells. Piper betle and Artocarpus heterophyllusextractshad a reducing effect on the nucleic acid and protein content in the larvae in a dose dependent manner whereas Artocarpus altilis extract did not exhibit any significant larvicidal activity. Piper betle and Artocarpus heterophyllusextractsshowed good insecticidal activity whereas A.altilis extract showed poor insecticidal activity. The results of the present study clearly indicate that Piper betle and Artocarpus heterophyllus extracts can be developed as ecofriendly larvicides and were also quite effective as insecticides for providing a better and excellent alternate for the control of insects.
This is the second in a series of presentations for students in the Department of Creative Professions and Digital Arts, University of Greenwich: How to think about social media presence, public and private, content, SEO, visibility, and how to write a good clickbait heading.
Effects of cytokinins and auxins on micropropagation of Musa spp. cv. YangambiAgriculture Journal IJOEAR
Abstract— The present study was conducted at Banana Tissue Culture lab of Regional Plant Resource Centre, Bhubaneswar, Odisha to obtain a standardized micropropagation protocol supplemented with different concentrations and combinations of cytokinins and auxins for Musa cv. Yangambi Km-5 (AAA) variety. Data collected for in vitro culture consists of the following parameters: days for bud initiation, rate of shoot proliferation (%), growth value (gm) and number of multiple shoot buds during multiplication stage. Remarkable effects of Cytokinins and Auxins were observed in Yangambi Km-5. Out of various treatments, best concentration for multiple shoot in short period of time for Yangambi Km-5 was found in mediums 3 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS and 1.5 mg/l BAP + 1.5 mg/l KN + 1 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS. Maximum growth value, rate of proliferation and number of shoot buds was obtained from explants culture medium 3 mg/l BAP + 0.5 mg/l IAA + 0.25 mg/l NAA + 100 mg/l ADS.
The use of biotechnology in the propagation of plantain and
banana (Musa sp.) of great importance to induce, tolerant to plant genotypes for
diseases and high yield potentials. However, auxins and cytokinins should be used,
which are expensive and can sometimes cause changes in the regenerants obtained.
Both traditional growth regulators (auxins and cytokinins) and non-traditional growth
regulators (brassinosteroid analogues and mixtures oligogalacturonide) are used in
the in vitro propagation of crops, but mush progress has been hindering due to the
sufficient knowledge and impact of different phases prevailing in the
micropropagation of banana hybrid 'FHIA-18' (AAAB) is present hitherto. This work
was performed in order to evaluate the biological activity of an analogue of
brassinosteroids (Biobras-6) *ABr+ and a mixture of oligogalacturonide with the degree
of polymerization between 9 and 16 (Pectimorf) *mOLG+. The effect of ABr and mOLG
are determined as a substitute or complement of auxin (IBA or IAA) and cytokinin (6-
BAP) for the establishment of in vitro multiplication and rooting of plantlets and in the
acclimatization phase. Non-traditional regulators phenolization decrease the explant
growth in the establishment phase of in vitro propagation; but increased the number
of shoots per explants (above 3.5) and improved survival of vitro plant during the
acclimatization phase.
Induced mutagenesis was carried out in an important protein rich pulse crop (Vigna radiata (L.) Wilczek). The seeds of green gram variety Co-6 were treated with different concentrations of sodium azide. The mutagen treated seeds were sown in the field to observe M1 characteristics. The sodium azide treated seeds were subjected to amino acid analysis. Totally 19 amino acids were recorded in control and sodium azide treated samples. In the process of sodium azide treatments a few amino acids were increased and some amino acids were decreased than control. The M1 parameters such as germination and survival percentage, plant height, days taken for flowering, number of pods/plant, length of pods, number of seeds/pod and hundred seeds weight were decreased with increasing concentrations sodium azide and all the growth parameters showed negative trend when compared to control. The M1 seeds were collected separately based on concentrations of sodium azide and stored for raising next generation after the harvest. The M1 seeds were sown in the field to raise M2 generation, and in M2 population, the different types of chlorophyll and viable mutants were noticed, such as chlorina, xantha, viridis, and viable mutants such as tall, dwarf, leaf, pod and early flowering mutants were noticed in various treatments of sodium azide. In addition with chlorophyll and viable mutants several initial leaflet modifications like trifoliate, tetrafoliate and pentafoliate leaflets had been observed in mutagenic treatment with sodium azide. The present study is a basis for evolving mutant varieties in green gram with altered agronomic traits.
Micropropagation of Santalum Album L. Sandalwoodijtsrd
An efficient plant regeneration protocol was developed for Santalum album L. Santalaceae , an economically important species. Plant regeneration was achieved using nodal explants and leaf disc on Murashige and Skoog MS medium for direct shoot regeneration. Effect of Plant Growth Regulators PGR like 6 Benzyl Adenine BA , Kinetin KN and 2 Isopentenyl adenine 2 iP on shoot initiation 2 Isopentenyl adenine and Gibberellic acid GA3 for shoot elongation and multiple shoot formation and Indole 3 Butyric Acid IBA and a Naphthalene Acetic Acid NAA for rooting was studied. Among the explants tested for shoot induction, nodal segments proved good results. The best treatment for obtaining shoot induction was 3.0mg L BAP and for rooting 1 mg L of IBA was found to be the best treatment combination for maximum sprouting of shoot and rooting. After six the rooted plantlets were transferred for hardening, 20 of plantlets survived and resumed growth in the mixture of soil, vermiculite and sand 1 1 1 . S. Aghi Zion Inbakani | S. Sathishkumar | Bakan Jagdish Sudhakar "Micropropagation of Santalum Album L. (Sandalwood)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43698.pdf Paper URL: https://www.ijtsrd.combiological-science/biotechnology/43698/micropropagation-of-santalum-album-l-sandalwood/s-aghi-zion-inbakani
The effects of explant rotation, medium types, JA and GA3 additions on in vit...Agriculture Journal IJOEAR
— This study investigates the effects on the in vitro microtuber formations of Solanum tuberosum L. cv. Marfona species, effected by the direction of planting the explants (horizontal or vertical), the type of medium (solid or two-phase), adding Jasmonic acid (JA) (0.0, 10 ng/L, 1 µg/L and 0.2 mg/L) and Gibberellic acid (GA 3) (0.0 and 0.2 mg/L). The cultures were incubated in a climate chamber at 22-25 o C, and were subjected to a light intensity of 145 µmol m-2 s-1 for 8 hours in light and 16 hours in dark photoperiods (short day) for 4-6 weeks. Microtuber production was inhibited when GA 3 was added. The maximum number of microtubers was observed when the explants were planted vertically and were grown in two-phase medium which did not contain GA 3 and had 10 ng/L JA present. It was determined that two-phase medium with 0.2 mg/L JA but without GA 3 was the most favourable medium for tuber growth (for both height and width). The best microtuber formation on single node explants were observed to occur in the short day photoperiod (8 hours light/16 hours dark) in a two-phase medium that contained 0.2 mg/L JA without the addition of GA 3. The results shows that the effect caused by JA works antagonistically with that of GA 3 thus causing the resulting microtuber formation observed.
Influence of Plant Growth Regulators on Somatic Embryogenesis Induction in Se...IJEABJ
Seriphidium herba-album (syn. Artemisia herba-alba) is a medicinal, aromatic, greenish-silver herb. It is used widely in folk medicine for treatment of diarrhea, abdominal cramps and in the healing of external wounds. It's also used for the treatment of diabetes mellitus, neurological disorders as epilepsy, Alzheimer’s disease, depression and jaundice. In this study we assessed the protocol for callus induction, maturation of somatic embryogenesis, frequency of germination and conversion into plantlets for leaf explants of Seriphidium herba-album using different concentrations of PGRs. Highest induction frequencies of embryogenic calli occurred after 35 days on MS medium supplemented with 1.5 mg L-1 2,4-D and 0.5 mg L-1 BAP. Optimum MS medium for higher frequency of matured somatic embryos was recorded using 5.0 mg L-1 BAP and 0.5 mg L-1 NAA and somatic embryos also induced young in vitro grown plantlets when cultured in the medium containing GA3 and kinetin. Hence, attempts to induce direct somatic embryogenesis have been achieved up to embryo regeneration and maturation.
Effect of Fermentation on the Nutritional and Antinutritional Composition of ...IOSR Journals
The dehulled seeds of three varieties of Lagenaria siceraria were subjected to control fermentation process. The fermented and unfermented seeds were analysed for their nutritional and anti-nutritional compositions using AOAC 1998. The fermented seeds were found to contain high amount of crude protein (48.12%) and crude fibre (4.11%) compared to 27.42% and 0.67% for unfermented seeds respectively. Similarly, crude lipid content of the seeds decreased by about 75%. The process also results in decrease in phytate, oxalate, tannins and cyanide content with consequent increase in nitrate and Vitamin C. Hence fermenting the seeds is an important way of exposing its protein content and reducing the antinutritional content. The seeds were found to have good potentials for preparation of condiments which are commonly used in the preparation of soup.
Physiological Assessments of Sweet Sorghum Inoculated with Azospirillumbrasi...Agriculture Journal IJOEAR
Abstract—Some factors, such as yield increase and production cost reduction, must still be assessed as a way toimprove the sweet sorghum success prospects. The use of plant growth regulator mixtures has shown significant results in crop yield.Nitrogen assimilation stands out as one of the main limiting factors in plant production. Given the physiological effects of plant growth regulators and their mode of action in the photosynthetic metabolism, the aim of the current study is to assess the physiological responses of sweet sorghum plants inoculated with Azospirillum brasilenseto the use of nitrogen fertilization and plant growth regulators. The experiment comprised split plots, with four repetitions. The treatments comprised nitrogen (0, 40, 80, 120 and 160 kg ha-1) and biostimulant levels (0, 300, 400, 500 and 600 mL ha-1) in sweet sorghum culture inoculated with Azospirillum brasilense. Variables such as gas exchange and total recoverable sugars (TRS%) were assessed. The application of plant growth regulator at the dose 440.7 ml ha-1 showed the highest photosynthetic efficiency up to 46 DAE. According to the experimental conditions, the plant growth regulator treatment had no effect on the TRS %. It is concluded that the plant growth regulator did not affect the production of sugars by the plant.
Screening Fenugreek genotypes for high callus induction and growth | IJAARInnspub Net
Fenugreek (Trigonella foenume-graecum) is a medicinal herb that has anti-diabetic properties. 4-hydroxyisoleucine, an important metabolite of this plant, is a good candidate for improving hepatic insulin resistance in type 2 diabetic patients. In vitro production of metabolites mainly relies on the ability of the tissue culture system to produce more and viable cells with maximum production rate. Callus induction is the first step of establishing plant cell based bioreactors which needs potential genotypes with higher and faster callus production. To find such genotypes, screening them is indispensable. In this research, we screened 21 Iranian landraces of fenugreek for callus induction and growth on MS medium supplemented with some plant growth regulators. The results showed different capacity of the screened genotypes in callus induction (P<0.05). Also, the type of explant had a significant effect on callus induction (P<0.05). Calli were able to produce 4-Hydroxyisulosine 67% of the amount in vivo samples.
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
Penelitian berkaitan dengan pengurangan intensitas cahaya akibat penutupan awan sbg bentuk pengaruh dari perubahan iklim thd kualitas benih dan perkecambahan kedelai
Physiological and biochemical response of common bean genotypes (Phaseolus vu...Premier Publishers
A field experiment was established over three rainy seasons (2011, 2012 and 2013) at two locations (Shaban and Al-Qaidah) which represented the severe (SDS) and moderate (MDS) drought stress in the southern highlands of Yemen-Ibb. The experiment was arranged in a split plot design with three replication. The main plots were: untreated or treated seeds with 0.5 mM salicylic acid (SA), the subplot were nine CIAT bean lines and three local cultivars. The results revealed that salicylic acid improved significantly the yield and yield traits of some genotypes. Accordingly, under severe drought stress, the bean genotypes categorized into three groups; The first group (MIB-156, MIB-156, G23818B and NSL) which were high yielding and low responsiveness genotypes to SA group (HY-LSAR); The second group (BFB-139, BFB-140 and BFB-141) that perform low yielding and high responsiveness genotypes to SA (LY-HSAR) and the third group (Taiz-304, Taiz-5 and Taiz-306) that perform low yielding and low responsiveness genotypes to SA group (LY-LSAR). It is concluded that the physiological mechanism of bean cultivars response of the high tolerant lines (LY-HSAR) and the medium tolerant lines to drought (LY-HSAR) to SA was similar by causing significant increase in dry matter accumulation, photosynthetic pigments content of leaves and accumulation of high proline content, total soluble sugars, total free amino acids, and soluble proteins, and also by maintain high relative water content (RWC%) and low leaf ion leakage (LIL%) in comparison to susceptible cultivars to drought (LY-LSAR).
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Growth Pattern, Molecular Identification and Bio molecules Analysis of FOMITO...journal ijrtem
Abstract : Fomitopsis feei, a brown rot fungus is identified tentatively using morphological characteristics and confirmed phylogenetically by 28S rDNA analysis and sequence was submitted in EMBL Nucleotide Sequence Database. Its growth pattern was studied on eight different solid media and found to be good on Malt extract agar medium. Biomolecules such as proteins and lipid were screened qualitatively and estimated quantitatively. Aminoacid analysis by chromatography and fatty acid analysis by FAME were also done and revealed that tryptophan (20.53%), valine (20.51%) and cis-linoleic acid (43.38%) and palmetic acid (17.88%) were in high percentage.
Key words : Fomitopsis feei, growth, molecular identification and biomolecules
Induced Mutagenesis of Flowering, Phenology and Yield in M1 Generation of Bam...ijtsrd
A highly cherished Bambara groundnut variety, ‘Caro’ was treated with different concentrations of ethyl methane sulphonate EMS for different durations to raise an M1 generation. In the M1 generation, the mutagenic effect of EMS on seed germination, growth and seed yield at different doses 0.01 , 0.1 , 0.25 and 0.5 and for different durations 6 hours, 12 hours and 24 hours were studied. Characters like days to first flowering, days to 50 flowering, number of flowers per plant, number of leaves, number of nodes per plant, number of stems per plant, plant spread, plant height, number of branches per plant, pod length, pod width, number of pods per plant, number of seeds per plant, seed length, seed width and seed yield were measured. Data collected were subjected to analysis of variance ANOVA and means were separated using least significant difference LSD . The result showed that there was significant effect p 0.05 of ethyl methane sulphonate concentrations in all the parameters except on number of days to maturity. The concentration, 0.1 and soaking duration of 6 hours was more promising in creating variability. Apparently, could Ethyl methane sulphonate significantly improved the performance and create genetic variability of Bambara groundnut and therefore can be employed in breeding programme for Bambara groundnut. Grace O. Chimdi | Michael I. Uguru | Peter. E. Ogbonna | Nwakuche C. Onwubiko "Induced Mutagenesis of Flowering, Phenology and Yield in M1 Generation of Bambara Groundnut (Vigna Subterrnea (L.) Verdc)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-2 , February 2022, URL: https://www.ijtsrd.com/papers/ijtsrd49327.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/49327/induced-mutagenesis-of-flowering-phenology-and-yield-in-m1-generation-of-bambara-groundnut-vigna-subterrnea-l-verdc/grace-o-chimdi
Isolation and characterization of plant growth promoting bacteria (PGPB) from...Agriculture Journal IJOEAR
— The use of anaerobic digestate as fertilizer is considered beneficial since it provides plant nutrients and organic matter to soils. However, there is limited information about plant growth promoting bacteria (PGPB) in digestate. In this study, we isolated Bacillus and Pseudomonas from two types of anaerobic digestates, and selected three different plant growth promoting traits and antifungal activity to screen 200 bacteria isolated from each digestate. Then 6 isolates based on plant growth promoting traits were selected and inoculated with common wheat seeds to evaluate their plant growth promoting activity. Cultivable population of Bacillus and Pseudomonas were 2.20 × 10 6 and 6.98 × 10 4 CFU g-1 dry matter in mesophilic digestate, while were 6.86 × 10 5 and 5.65 × 10 4 CFU g-1 dry matter in thermophilic digestate. Twenty-five bacterial isolates from mesophilic digestate and 12 bacterial isolates from thermophilic digestate showed positive plant growth promoting traits or antifungal activity. In plant growth promoting assay, all isolates significantly promoted growth of wheat seedlings (p < 0.05). Seedlings stem length was increased from 28.5% to 38.6% by bacteria inoculation. In addition, bacteria inoculation increased seedlings stem weight from 113.3% to 214.2% and root weight from 108.6% to 207.2% as compared to un-inoculated control. The results showed that anaerobic digestate was a potential source for isolation of PGPB, and PGPB in digestate would be beneficial for plant growth with fertilizer application.
Evaluation of seed storage proteins in common bean by some biplot analysisINNS PUBNET
In order to study of seed storage proteins, proteins samples of common bean genotypes were prepared by 0.2 M
NaCl of extracting soluble. Genotypes were located in two groups by cluster analysis using Wilks’ lambda
statistic. Two groups were different for yield components (number of pods per plant, number of seeds per plant
and seed weight). Factor analysis showed that two factors described 61% of total proteins variation. Correlated
bands with yield components characters had the highest coefficients for the first factor. This factor was named
“yield components proteins”. Protein bands via RM 58 and 64 had relationship with days to flowering.
Therefore, the second factor was named “phenologic proteins”. Genotypes were located in four groups by these
factors. Length, angle and presence of protein bands were important characteristics to explain graphical
information in GGE biplot compared to factor analysis. Get the full articles at: http://www.innspub.net/volume-3-number-5-may-2013/
Similar to Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induction and Somatic Embryogenesis in Sesame (Sesamum indicum L.) (20)
Natural farming @ Dr. Siddhartha S. Jena.pptxsidjena70
A brief about organic farming/ Natural farming/ Zero budget natural farming/ Subash Palekar Natural farming which keeps us and environment safe and healthy. Next gen Agricultural practices of chemical free farming.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
Follow us on: Pinterest
Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
Diabetes is a rapidly and serious health problem in Pakistan. This chronic condition is associated with serious long-term complications, including higher risk of heart disease and stroke. Aggressive treatment of hypertension and hyperlipideamia can result in a substantial reduction in cardiovascular events in patients with diabetes 1. Consequently pharmacist-led diabetes cardiovascular risk (DCVR) clinics have been established in both primary and secondary care sites in NHS Lothian during the past five years. An audit of the pharmaceutical care delivery at the clinics was conducted in order to evaluate practice and to standardize the pharmacists’ documentation of outcomes. Pharmaceutical care issues (PCI) and patient details were collected both prospectively and retrospectively from three DCVR clinics. The PCI`s were categorized according to a triangularised system consisting of multiple categories. These were ‘checks’, ‘changes’ (‘change in drug therapy process’ and ‘change in drug therapy’), ‘drug therapy problems’ and ‘quality assurance descriptors’ (‘timer perspective’ and ‘degree of change’). A verified medication assessment tool (MAT) for patients with chronic cardiovascular disease was applied to the patients from one of the clinics. The tool was used to quantify PCI`s and pharmacist actions that were centered on implementing or enforcing clinical guideline standards. A database was developed to be used as an assessment tool and to standardize the documentation of achievement of outcomes. Feedback on the audit of the pharmaceutical care delivery and the database was received from the DCVR clinic pharmacist at a focus group meeting.
"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
Micro RNA genes and their likely influence in rice (Oryza sativa L.) dynamic ...Open Access Research Paper
Micro RNAs (miRNAs) are small non-coding RNAs molecules having approximately 18-25 nucleotides, they are present in both plants and animals genomes. MiRNAs have diverse spatial expression patterns and regulate various developmental metabolisms, stress responses and other physiological processes. The dynamic gene expression playing major roles in phenotypic differences in organisms are believed to be controlled by miRNAs. Mutations in regions of regulatory factors, such as miRNA genes or transcription factors (TF) necessitated by dynamic environmental factors or pathogen infections, have tremendous effects on structure and expression of genes. The resultant novel gene products presents potential explanations for constant evolving desirable traits that have long been bred using conventional means, biotechnology or genetic engineering. Rice grain quality, yield, disease tolerance, climate-resilience and palatability properties are not exceptional to miRN Asmutations effects. There are new insights courtesy of high-throughput sequencing and improved proteomic techniques that organisms’ complexity and adaptations are highly contributed by miRNAs containing regulatory networks. This article aims to expound on how rice miRNAs could be driving evolution of traits and highlight the latest miRNA research progress. Moreover, the review accentuates miRNAs grey areas to be addressed and gives recommendations for further studies.
Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induction and Somatic Embryogenesis in Sesame (Sesamum indicum L.)
1. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-7, July- 2016]
Page | 68
Influence of Plant Growth Regulators and Explant Type on
Multiple Shoot Induction and Somatic Embryogenesis in Sesame
(Sesamum indicum L.)
Firoozeh Chamandoosti
Iranian research Institue of Plant protection, PhD of Cellular and Developmental Biology, Assistant Professor of Iranian
Research Institute of Plant Protection Department of Plant Diseases
Corresponding author: fychamandoosti@yahoo.com
Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid
system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent
combinations and concentrations of PGRs. On medium with 5 mgl-1
BA plus 1 mgl-1
IAA and 1 mgl-1
ABA mutliple shoot
induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1
BA and 0.3 mgl-1
NAA
happen with a short phase of callusing. Also MS medium with 3 mgl-1
2,4-D and 0.5 mgl-1
kinetin was a suitable medium for
inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media
rooted on MS medium plus 1.5 mgl-1
NAA and 0.03 mgl-1
BA and whole regenerated plantlets formed.
Abbreviations: 2,4-D – 2,4-dichlorophenoxy acetic acid; ABA – Abscisic acid; BA – 6 benzylaminopurine; IAA – Indole – 3
– acetic acid; IBA – Indole – 3 – butyric acid; Kinetin – n6
– Furfyrylaminopurine; MS – Murashige and Skoog medium;
NAA – Naphtalene acetic acid; PGRs – Plants Growth Regulators.
Key words: adventitious shoots, embryogenic calli, sesame.
I. INTRODUCTION
Sesame (Sesamum indicum L.) is an important crop in tropical and subtropical areas. This important crop ranks third among
the oilseed in production. Its oil content varies from 44 – 66 % containing two unsaturated fatty acids – oleic and linoleic
together account for 85% (Maximum) with a combination of different essential amino acids and vitamins particularly β
carotene (Brar, 1982 and Arslan et al., 2007). Sesame cultivated in Iran in central, north – western, north – eastern, western
and eastern parts. Also sesame is one of the most desirable oils due to demanded perfume and demanded flavour in our
country. Cultivation of sesame suffers from considerable yield loss because of pathogenic diseases like phytophthora blight
and root/stem rot (Gangopadhyay et al., 1998). In addition, it is difficult to the time of harvest a sesame crop to maximize
yield because plant growth is indeterminate and capsules dehisce when mature (Day, 2000). Conventional methods for
improvement is slow, felt difficult, owing to nonavailability of desired genetic variation in cultivated types. Though a few
wild species are known to carry resistance genes, it is difficult to transfer owing to presence of inter-specific barriers
(Shashidhara et al., 2011).
Biotechnological approach like exploitation through somaclones, genetic transformation requires a dependable, flexible and
reproducible callus initiation and shoots regeneration system (Shashidhara et al., 2011). So in the present investigation, we
made an attempt to use different plant growth regulators and different explants for callus induction and producing somatic
embryo and regenerated organs (shoot and root) in sesame.
II. MATERIALS AND METHODS
Seeds of sesame CV. Darab1 were obtained from Seed and Plant Improvement Institute, Karaj, Iran.
Mature seeds of S. indicum L. were aseptically sterilized by immersing in 100% commercial hypochlorite sodium (with 5%
available chlorine) for 30 min. After washing with sterilized distilled water for 4 – 5 times, the seeds were cultured on half –
strenght Murashige and skoog (MS) (Murashige and Skoog, 1962) medium, MS medium, half – strenght Gamborg medium
(B5) (Gamborg, 1968) and B5 medium with 30 g l- 1
sucrose and 7.5 g l- 1
bacto agar. Hypocotyl (4 – 5 mm) and shoot tip (2
– 3 mm) explants from 1 week – old seedlings cultured on MS medium including different kind and concentrations of PGRs
(IBA, BA, NAA, KIN, IAA, ABA, 2,4-D) and with 100 mg l- 1
m-inositol, 0.1 mg l- 1
thiamine, 0.5 mg l- 1
pyridoxine, 0.5
mg l- 1
nicotinic acid, 2 mg l- 1
glycine, 30 g l- 1
sucrose and 4 g l- 1
phytogel. All media adjusted to pH 5.8 before
autoclaving at 120°C for 15 min. The culture room was maintained at 24 ± 1°C with a 16-h photoperiod and 50 µmol m-2
s-1
using cool – white fluorescent tubes. 10 hypocotyl explants and 5 shoot tip explants were cultured into 10-cm petri dishes
2. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-7, July- 2016]
Page | 69
per treatment and the experiment was repeated 5 times. After about 1 month the induction of adventitious shoots and
embryogenic calli were considered.
III. RESULTS AND DISCUSSION
3.1 Effect of basal media on growth and development of seedlings
Seed germination is an important steps of establishing plant tissue culture, because quality of growth and development of
seedlings influence to have a uniform and suitable set of seedlings to the treatments (Sakhanokho et al., 2001). Among of
different basal media that were evaluated for their effects on seed germination, half strength MS was the best medium based
on longitudinal and trabsversal growth of seedlings (data were not shown) and rate of germination. That's a thought different
between basal media are depended their salt concentrations. As mentioned earlier the half strength MS medium was the best
medium for sesame seed germination. Our results agree with results of Droste et al., 2005 and Abdellatef and Khalafalla,
2007 that stressed in low salt-concentration media the highest in vitro germination rate was performed. Because in half
strength MS medium the concentrations of salt are half.
3.2 Effect of PGRs and shoot tip explants on adventitious shoot formation
The shoot tip explants from 7 – days – old seedlings were cultured on MS medium with various kinds and concentrations of
PGRs, alone or combination with others (Table 1). The best combination of PGRs for the highest rate of adventitious shoot
formation was 5 mgl-1
BA plus 1 mgl-1
IAA and 1 mgl-1
ABA (Fig. A1). Also on medium with 2 mgl-1
BA and 0.3 mgl-1
NAA
either rate or the numerous adventitious shoot per explants was considerable (Fig. A3).
Other media such as the combination of BA (0.1 mgl-1
) and IAA (1 mgl-1
) and that were pointed in table 1 was significantly
less responsive for adventitious shoot formation (Fig. A4).
The most important characteristic of these two media is high level of BA. The positive effect of BA on the induction of
regenerated shoot is clear. For example the positive effect of BA on shooting in Cucurbita maxima Duch (Lee el al., 2003),
Ruta graveolunse L. (Ahmad, N. et al., 2010), tomato (Lycopersicon esculentum L.) (Rai et al., 2012) and Japanese pear
(Kadota et al. 2001).
On medium with 2 mgl-1
BA and 0.3 mgl-1
NAA hypocotyls segments produced callus at low frequency, then produced
adventitious shoots (as could be seen in Fig. A2). But on medium with 5 mgl-1
BA plus 1 mgl-1
IAA and 1 mgl-1
ABA
callusing phase was very short and explants produced adventitious shoots directly. So the results of present study provide a
rapid and proper system which could be used for either somaclonal variation induction or direct induction of organs and
elimination effects of somaclonal variation.
TABLE 1
EFFECT OF DIFFERENT CONCENTRATIONS AND COMBINATIONS OF PGRS ON ADVENTITIOUS SHOOT
FORMATION FROM SHOOT TIP OF 7 – DAYS - OLD SEEDLINGS IN S. INDICUM L.
IAA
mgl-1
BA
mgl-1
ABA
mgl-1
NAA
mgl-1
Frequency of
shoot formation
(%)
The mean No. of
shoots/explants
1 0.1 1 0 30 2
1 1 1 0 30 2
1 2 1 0 27 2
1 3 1 0 45 3
1 4 1 0 _ 2
1 5 1 0 98 15
0 0.1 0 0 12 3
0 1 0 0.1 12 3
0 2 0 0.2 19 2
0 2 0 0.3 95 11
3. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-7, July- 2016]
Page | 70
3.3 Effect of PGRs and hypocotyls explants on somatic embryogenesis
Hypocotyl explants from 7 – days – old sesame seedlings cultured on MS media supplemented with different concentrations
and combinations of PGRs. 2, 4-D plus kinetin was the best combination for highest rate of callusing (with somatic
embryogenesis characteristic calli) (Table 2.). According to researchers hypocotyls are considered as model explant for callus
induction (Shashidhara et al., 2011). Also in present study hypocotyls explants induced calli on media nearly 20 – 30 days
after establishing. In fact 5 – 7 days after establishing of explants on media the cut surfaces of them became swollen then the
callusing was begun slowly.
It is clear that initiation of embryogenic cultures start by culturing the primary explant on medium supplemented with PGRs,
mainly auxin but often also cytokinin (Von alnold et al., 2002). In our study 3 mgl-1
2,4-D and 0.5 mgl-1
kinetin was the best
combination of PGRs for inducing the embryogenic calli in sesame (Fig. A6). Also about the role of 2,4-D in somatic
embryogenesis scientists believed that the use of 2,4-D alone or in combination with other hormones has become almost
routine in somatic embryogenesis. (Huang and Yeoman, 1984; Mordhorst et al., 2002). All of these declarations agree with
our results.
TABLE 2
EFFECT OF DIFFERENT CONCENTRATIONS AND COMBINATIONS OF PGRS ON INDUCTION OF SOMATIC
EMBRYOGEN CALLI FROM SHOOT TIP OF 7 – DAYS - OLD SEEDLINGS IN S. INDICUM L
2,4-D BA Kinetin
The mean of diameter of calli
(mm)
Color of calli
The percentage of
somatic embryogenic
calli (%)
3 1 0 5 Brownish yellow 22
3 0 0.5 9 Brownish yellow 57
3 0 0 4.4 Brownish yellow 20
4 0 0 5 Brownish yellow 20
In addition to callus induction, callus maintenance is an important step also and must to be standardized (Shashidhara et al.,
2011). General observation is that the callus turn brown after about more than five cycles of sub-culturing (Khanna, 1999).
Whereas as could be seen in figures (Fig. A7 and A8) calli from above mentioed explants and above mentioned media have
potential of numerous sub-culturing. These figures show the successful subculture of callus from above mentioned medium
after 4 times and for a 2 month period.
3.4 Rooting and production of whole plantlets
Regenerated shoots were transferred to MS medium supplemented with kinds of auxins such as IAA, IBA, and NAA. NAA
were used either alone or with 0.03 mgl-1
BA. Other auxins were added media from 1 – 1.5 mgl-1
. The results showed that the
combination of NAA and BA (1.5 mgl-1
NAA and 0.03 mgl-1
BA) has the best effect on rooting the bases of regenerated
shoots and formation of whole plantlets (Fig. A5). There are many reports that showed the combination of NAA and BA is
the most effective combination for rooting in sesame (Chakraborti and Ghosh, 2010), (Raja and Jayabalan, 2011). Also there
are reports about the positive effect of NAA alone on rooting of sesame (Kushwaha et al., 2010). So much is certain that the
NAA is the best PGRs for In Vitro rooting in many of plants.
In conclusion we established a simple and rapid regeneration system in sesame via both direct and indirect adventitious
shoots. Also we presented a system for callusing and sub-culturing of calli in this reculcitrant plant.
4. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-7, July- 2016]
Page | 71
FIG . A ADVENTITIOUS SHOOTS AND SOMATIC EMBRYOGENIC CALLI FORMATION FROM SHOOT TIPS AND HYPOCOTYLS
EXPLANTS IN 7 – DAYS – OLD SEEDLINGS OF SESAME. (1) DIRECT ADVENTITIOUS SHOOT ON MS MEDIUM WITH 5 mgl-1
BA
plus 1 mgl-1
IAA and 1 mgl-1
ABA. (2) AND (3) INDIRECT ADVENTITIOUS SHOOT ON MS MEDIUM WITH 2 mgl-1
BA and
0.3 mgl-1
NAA. (4) SHOOT FORMATION FROM HYPOCOTYL EXPLANTS ON MS MEDIUM WITH 0.1 mgl-1
BA and 1 mgl-1
IAA. (5) ROOT FORMATION ON BASES OF ADVENTITIOUS SHOOT ON MS MEDIUM WITH 1.5 mgl-1
NAA AND 0.03 mgl-1
BA. (6) EMBRYOGENIC CALLI ON HYPOCOTYL EXPLANTS ON MS MEDIUM WITH 3 mgl-1
2,4-D AND 0.5 mgl-1
KINETIN. (7)
AND (8) EMBRYOGENIC CALLI ON HYPOCOTYL EXPLANTS ON MS MEDIUM WITH 3 mgl-1
2,4-D and 0.5 mgl-1
KINETIN
AFTER 4 TIMES AND FOR A 2 MONTH PERIOD
REFERENCES
[1] Abdellatef E, and Khalafallah M.M. 2007. Adventitious shoot formation and plant regeneration in medium staple cotton (Gossypium
hirsitum L.) cultivar (Barac B-67). Int. J. Agri. Biol. 9(6): 913- 916
[2] Ahmad N, Faisal M, Anis M, and Aref I.M. 2010. In Vitro callus induction and plant regeneration from leaf explants of Ruta
graveolense L. South Afica Journal. 76(3): 597 – 600
[3] Arslan C,Uzun B, Ulger S, and Cagirgan M.I. 2007. Determination of oil content and fatty acid composition of sesame mutants suited
for intensive management conditions. J. Am. Oil Chem. Soc. 84: 917 – 920
5. International Journal of Environmental & Agriculture Research (IJOEAR) ISSN:[2454-1850] [Vol-2, Issue-7, July- 2016]
Page | 72
[4] Brar G.S, 1982. Variations and correlations in oil content and fatty acid composition of sesame. Indian J. Agric. Sci. 52: 434 – 439
[5] Chakraborti P, and Ghosh A. 2010. Variation in callus induction and root – shoot bud formation depend on seed coat of sesame
genotype. Research Journal of Botany. 5(1): 14 – 19
[6] Day S.J. 2000. Development and maturation of sesame seeds and capsules. Field Crops Res. 67: 1 – 9
[7] Droste A, Machado S.A, Matos V.A, and Almeida W.J. 2005. In Vitro Culture of Vriesea gigantean and Vriesea Philippocoburgii:
Two Vulnerable Bromeliads Native to Southern Brazil. Brazilian Arch. Biol. Technol. 48: 717-22
[8] Gamborg O. L, Miller R.A, and Ojima K. 1968. Nutrition requirements of suspension cultures of soybean root cells. Expt. Cell. Res.
50: 151 – 158
[9] Gangopadhyay G, Poddar R, and Gupta S. 1998. Micropropagation of sesame (Sesamum indicum L.) by In Vitro multiple shoots
production from nodal explants. Phytomorphology. 48: 83 – 90
[10] Huang B.C, Yeoman M.M. 1984. Callus proliferation and morphogenesis in tissue cultures of Arabidopsis thaliana L. Plant Science
Letters 33: 353 – 363
[11] Kadota M, Lmizu K, and Hirano T. 2001. Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces
hyperhydricity in Japanese pear. Scientica Horticulturae. 89(3): 207 – 2015
[12] Khanna V.K. 1999. Plant Tissue Culture Practices, Kalyani Publishers. New Delhi
[13] Kushwaha D.S, Khan S, and Hasan Z.U. 2010. In Vitro Micropropagation and Mass Multiplication of Sesame (Sesamum Indicum L.)
From Apical and Axillary Meristem. International Journal of Agriculture and Food Science Technology. 1(1): 13 – 22
[14] Lee Y.K, Chung W, and Ezura H. 2003. Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch.).
Plant Science. 164(3): 413 – 418
[15] Mordhorst A. P, Hartog M,V Tamer M. K. El, Laux T, and Vries S. C. de. 2002 Somatic embryogenesis from Arabidopsis shoot
apical meristem mutants. Planta .214(6) : 829-836
[16] Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Phyiol. Plant. 15:
473 – 497
[17] Rai G.K, Rai N.P, Kumar S, yadav A, and Rathaur S. 2012. Effects of explant age, germination medium, pre-culture parameters,
inoculation medium, pH, washing medium, and selection regime on Agrobacterium-mediated transformation of tomato. In Vitro
Cellular and Developmental Biology. 48: 565 – 578
[18] Raja A, and Jayabalan N. 2011. In vitro shoot regeneration and flowering of Sesame (Sesamum indicum L.) cv. SVPR – 1. 7(4): 1089
– 1096
[19] Sakhanokho H.F, Zipf A, Rajasekaran K, SahaS, and Sharma G.C. 2001. Induction of highly embryogenic calluses and plant
regenertion in Upland and Pima cottons. Crop Sci., 41: 1235-1240
[20] Shashidhara N, Ravikumar H, Ashoka N, Santosh D. T, Pawar P, Lokesha R, and Janagoudar B. S. 2011. Callus induction and sub-
culturing in Sesame. International Journal of Agricultural, Environmentral, and Biogeochemistry. 4(3): 153 – 156
[21] Von Arnold S, Sabala I, Bozhkov P, Dyachok J, and Filonova L. 2002. Developments pathways of somatic embryogenesis. Plant Cell
Tissue and Organ Culture. 69: 233 – 249