This document discusses a study that evaluated the effect of different concentrations of glycerol on the survival of the nitrogen-fixing bacterium Azotobacter chroococcum in liquid formulations. Azotobacter was inoculated into Jensen's broth containing various concentrations of glycerol from 5 mM to 25 mM. The number of surviving Azotobacter colonies was counted over 360 days. Results showed that survival was highest in the 25 mM glycerol formulation, with 22 colonies surviving after 360 days. Survival was lowest in the 5 mM glycerol formulation, with only 5 colonies surviving after 360 days. Additionally, three Azotobacter strains showed different survival rates in the 25 mM glycerol formulation over 360 days, demonstrating an
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
THE EFFECTS OF HELPING BACTERIA (PSEUDOMONAS SPP.) IN NITROGEN GREEN BEANS F...IJSIT Editor
Some- bacteria settle in the rhizosphere of legume plants and enhance the performance of ribosome
bacteria to nitrogen fixation and nodulation. In this paper, we used four isolated from two species of
Pseudomonas containing P.putida, P.fluorescens Chao, P.Flouresence Tabriz, P.flouresence B119 and Rhizobium
leguminosarumbv.phaseoli. In a factorial experiment with complete randomized blocks were used 5 levels of
helping bacteria(Pseudomonas spp.) and two rhizobium levels, four replicates were employed. Jamaran418
green bean was utilized as host plant. At the end, nodulation, growth and plant’s nitrogen indexes were
measured. The results showed that all above mentioned helping bacteria enhance the growth and nodulation
performance of green bean. It should be said that P.putida had the highest effect on the green bean
nodulation increase along with rhizobium (130%) followed by P.fluorescens Tabriz, P. fluorescens Chao and
P.fluorescens B119, ( 83, 63 and 17%, respectively). Also, we observed 45, 33, 22 and 8% performance
increase under the effect of P.putida, P. fluorescens Chao, P. fluorescens Tabriz and P. fluorescens B119,
respectively.
Isolation and characterization of plant growth promoting bacteria (PGPB) from...Agriculture Journal IJOEAR
— The use of anaerobic digestate as fertilizer is considered beneficial since it provides plant nutrients and organic matter to soils. However, there is limited information about plant growth promoting bacteria (PGPB) in digestate. In this study, we isolated Bacillus and Pseudomonas from two types of anaerobic digestates, and selected three different plant growth promoting traits and antifungal activity to screen 200 bacteria isolated from each digestate. Then 6 isolates based on plant growth promoting traits were selected and inoculated with common wheat seeds to evaluate their plant growth promoting activity. Cultivable population of Bacillus and Pseudomonas were 2.20 × 10 6 and 6.98 × 10 4 CFU g-1 dry matter in mesophilic digestate, while were 6.86 × 10 5 and 5.65 × 10 4 CFU g-1 dry matter in thermophilic digestate. Twenty-five bacterial isolates from mesophilic digestate and 12 bacterial isolates from thermophilic digestate showed positive plant growth promoting traits or antifungal activity. In plant growth promoting assay, all isolates significantly promoted growth of wheat seedlings (p < 0.05). Seedlings stem length was increased from 28.5% to 38.6% by bacteria inoculation. In addition, bacteria inoculation increased seedlings stem weight from 113.3% to 214.2% and root weight from 108.6% to 207.2% as compared to un-inoculated control. The results showed that anaerobic digestate was a potential source for isolation of PGPB, and PGPB in digestate would be beneficial for plant growth with fertilizer application.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated ...Agriculture Journal IJOEAR
— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal variation that occurred in many different crops. In this study a program for disease resistance was established in sesame using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth Regulators were tested for producing of plantlet regenerated from apical shoot explants. The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Study on the Use of Agricultural Wastes for Cellulase Production by Using Asp...IOSR Journals
It was the goal to investigate the Cellulase enzyme production ability of fungal strains such as
Aspergillus niger against the lignocellulosic bio-waste like Rice husks, Millet husks, Maize cobs, Wheat straws
and leaves at varying environmental parameters of pH, substrate concentration and dry weight and wet weight.
The enzyme production was analyzed individually by Miller‘s modified method of Dinitrosalicylic acid ( DNSA
). In this study the high level of enzyme production was achieved at pH 3 by using Rice husks, Millet husks,
Maize cobs, Wheat straws and leaves and pH 4 by using Maize cobs at varying substrate concentrations. Out of
all the five used substrates rice husks gave the higher production of the cellulose enzyme. The importance of
cellulase enzyme in industries cannot be over emphasized.
The Studies of Effect of Bio Fertilizers Rhizobium, Phosphobacteria, and Root...IIJSRJournal
A pot culture experiment was carried out and to the find out the effect of Rhizobium, Phosphobacteria and Root Nodule extract on the vegetative growth and biochemical changes in Black gram and Maize. The Black gram and Maize is vegetative parameters such as seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight had analyzed and biochemical studies of both Black gram and Maize was carried such as total chlorophyll content, leave soluble protein and in vivo nitrate reductase activity. Among microbial inoculants, the Rhizobium + PB mixer was found most effective in terms of seed germination, shoot and root length, leaf fresh weight, leafs dry weight, shoot and root dry weight and also showed increase total chlorophyll content, soluble protein and nitrate activities in both Black gram and Maize.
THE EFFECTS OF HELPING BACTERIA (PSEUDOMONAS SPP.) IN NITROGEN GREEN BEANS F...IJSIT Editor
Some- bacteria settle in the rhizosphere of legume plants and enhance the performance of ribosome
bacteria to nitrogen fixation and nodulation. In this paper, we used four isolated from two species of
Pseudomonas containing P.putida, P.fluorescens Chao, P.Flouresence Tabriz, P.flouresence B119 and Rhizobium
leguminosarumbv.phaseoli. In a factorial experiment with complete randomized blocks were used 5 levels of
helping bacteria(Pseudomonas spp.) and two rhizobium levels, four replicates were employed. Jamaran418
green bean was utilized as host plant. At the end, nodulation, growth and plant’s nitrogen indexes were
measured. The results showed that all above mentioned helping bacteria enhance the growth and nodulation
performance of green bean. It should be said that P.putida had the highest effect on the green bean
nodulation increase along with rhizobium (130%) followed by P.fluorescens Tabriz, P. fluorescens Chao and
P.fluorescens B119, ( 83, 63 and 17%, respectively). Also, we observed 45, 33, 22 and 8% performance
increase under the effect of P.putida, P. fluorescens Chao, P. fluorescens Tabriz and P. fluorescens B119,
respectively.
Isolation and characterization of plant growth promoting bacteria (PGPB) from...Agriculture Journal IJOEAR
— The use of anaerobic digestate as fertilizer is considered beneficial since it provides plant nutrients and organic matter to soils. However, there is limited information about plant growth promoting bacteria (PGPB) in digestate. In this study, we isolated Bacillus and Pseudomonas from two types of anaerobic digestates, and selected three different plant growth promoting traits and antifungal activity to screen 200 bacteria isolated from each digestate. Then 6 isolates based on plant growth promoting traits were selected and inoculated with common wheat seeds to evaluate their plant growth promoting activity. Cultivable population of Bacillus and Pseudomonas were 2.20 × 10 6 and 6.98 × 10 4 CFU g-1 dry matter in mesophilic digestate, while were 6.86 × 10 5 and 5.65 × 10 4 CFU g-1 dry matter in thermophilic digestate. Twenty-five bacterial isolates from mesophilic digestate and 12 bacterial isolates from thermophilic digestate showed positive plant growth promoting traits or antifungal activity. In plant growth promoting assay, all isolates significantly promoted growth of wheat seedlings (p < 0.05). Seedlings stem length was increased from 28.5% to 38.6% by bacteria inoculation. In addition, bacteria inoculation increased seedlings stem weight from 113.3% to 214.2% and root weight from 108.6% to 207.2% as compared to un-inoculated control. The results showed that anaerobic digestate was a potential source for isolation of PGPB, and PGPB in digestate would be beneficial for plant growth with fertilizer application.
Comparative analysis of some biochemical responses of winter and spring wheat...Innspub Net
To compare changes of biochemical indices between spring (Kavir) and winter (Azar2) cultivars of wheat (Triticum aestivum L.) under low temperature, 14 days old wheat seedlings were exposed to cold. The seedlings were transferred into growth chamber for 9 days at 5/3 °C (day/night) as cold treatment, or at 20/18 °C as control. Proline content, total protein accumulation, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) enzymes, were assayed in the leaf extracts of control and cold treated plants. The results showed that cold led to an accumulation of proline and an increase in protein level, especially in winter cultivar. Rapid increases in proline and protein accumulations were observed during early stages of cold stress. SOD activity displayed no significant differences between the two cultivars during the first 3 days after cold stress, while in Azar 2, the level of SOD activity was gradually increased after 3 days of cold stress. The POD and CAT activity were higher in plants grown at cold stress than in the controls; however, their rate was different in winter and spring wheat cultivars. In general, Azar2 showed relatively higher POD and CAT activity compared to Kavir. Regarding antioxidant enzymes activities, cultivars respond differently under cold stress. Articles source: http://www.innspub.net/volume-7-number-4-october-2015-ijaar/
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Isolation, identification of antagonistic rhizobacterial strains obtained fro...Shazia Shahzaman
Plant growth promoting rhizobacteria (PGPR), are associated with roots, found in the rhizosphere and can directly or indirectly enhance the plant growth. In this study soil was collected from rhizosphere of chickpea fields of different areas of Rawalpindi division of Pakistan. PGPR were isolated, screened and characterized. Eight isolates of rhizobacteria (RHA, RPG, RFJ, RC, RTR, RT and RK) were isolated from Rawalpindi division and were characterized. The antagonistic activity of these PGPR isolates against root infecting fungi (Fusarium oxysporum and Verticillium spp.,) was done and production of indole acetic acid (IAA), siderophore and P-solubilization was evaluated. The isolates RHA, RPG, RFJ, RC, RRD and RT were found to be positive in producing siderophore, IAA and P-solubilization. Furthermore, most of the isolates showed antifungal activity against Fusarium oxysporum, and Verticillium spp. The rhizobacterial isolates RHA, RPG, RFJ, RC, RRD, RTR, RT and RK were used as bio-inoculants that might be beneficial for chickpea cultivation as the rhizobacterial isolates possessed the plant growth promoting characters i.e. siderophore, IAA production, phosphate solubilization. In in vitro tests, Pseudomonas sp. and Bacillus spp. inhibited the mycelial growth of the fungal root pathogens. The isolates (RHA and RPG) also significantly increased (60-70%) seed germination, shoot length, root length of the chickpea. The incidence of fungi was reduced by the colonization of RHA and RPG which enhanced the seedling vigor index and seed germination. The observations revealed that isolates RHA and RPG is quite effective to reduce the fungal root infection in greenhouse, and also increases seed yields significantly. These rhizobacterial isolates appear to be efficient yield increasing as well as effective biocontrol agent against fungal root pathogen.
Comparison of Resistance to Fusarium wilts disease in Seeded and Regenerated ...Agriculture Journal IJOEAR
— Plant tissue culture has been used as a tool for crop improvement in many different ways. Such as somaclonal variation that occurred in many different crops. In this study a program for disease resistance was established in sesame using somaclonal variation. As resistance to Fos is very important so different kinds and concentrations of Plant Growth Regulators were tested for producing of plantlet regenerated from apical shoot explants. The results showed that the combination of BA and NAA also BA and IAA with ABA could be used for regenerating sesame plantlets from apical shoots. The difference in BA concentrations had a positive effect on shoot and root regeneration and at least plant regeneration. So with combination of high level of BA and low level of NAA shooting from explants was dominant and with low level of BA and high level of NAA rooting was progressed. Regenerated plantlets and seeded planlets were compared for examining of resistance or susceptibility to Fos. The result showed that somaclonal variant resulted from regeneration of shoot and root of sesame could lead to producing resistant plantlets.
Study on the Use of Agricultural Wastes for Cellulase Production by Using Asp...IOSR Journals
It was the goal to investigate the Cellulase enzyme production ability of fungal strains such as
Aspergillus niger against the lignocellulosic bio-waste like Rice husks, Millet husks, Maize cobs, Wheat straws
and leaves at varying environmental parameters of pH, substrate concentration and dry weight and wet weight.
The enzyme production was analyzed individually by Miller‘s modified method of Dinitrosalicylic acid ( DNSA
). In this study the high level of enzyme production was achieved at pH 3 by using Rice husks, Millet husks,
Maize cobs, Wheat straws and leaves and pH 4 by using Maize cobs at varying substrate concentrations. Out of
all the five used substrates rice husks gave the higher production of the cellulose enzyme. The importance of
cellulase enzyme in industries cannot be over emphasized.
Identification and Evaluation of Antifungal Compounds from Botanicals for th...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus,
Colletotrichum
falcatum
. In this study, eighteen different botanicals were screened for
identifying effective antifungal compound against
C.
falcatum.
Among the plants
screened, 15 per cent aqueous leaf extract of
Psoralea corylifolia
alone inhibited 100
per cent growth of both mycelium as well as spore germination under
in vitro
conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes
viz.
,
Pseudomonas fluorescens
,
Bacillus megaterium
and
Gluconacetobacter
diazotrophicus
which are normally used in sugarcane. The effective plant extracts
exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC
-
MS
analysis to identify the bioactive antifungal compound. It revealed the
presence of
7H
-
furo [3,2
-
G] (1) benzopyran
-
7
-
one as main bioactive compound which is thought to be
the intermediate of antifungal compound, 8
–
methoxypsoralen formed during
biosynthesis.
Plant Tissue culture part II by Dr. Preeti VermaPreeti Verma
This presentation is meant only for educational purpose and includes various aspects of Plant Tissue culture in brief, including Media, Requirements, Problems in PTC, Techniques, Basic requirements for PTC LAB, Adavantages and Applications
DOI: 10.21276/ijlssr.2016.2.4.7
ABSTRACT- Most of the Euphorbian plants secrete fluid which contain a proteolytic enzyme for defensive role against
insects, pests and hence eco physiological inheritance to sustain vegetation eventually in adverse environmental
conditions. Evaluation has been carried out on twenty five Euphorbian garden plants for their proteolytic activities using
casein as a substrate. Out of these, Euphorbia nerifolia, Euphorbia milli, Euphorbia tirucalli, Euphorbia lactea,
Synadenium granti, Jatropha curcas, Euphorbia nivulia, Euphorbia antiquoram, Pedilanthus tithymaloides, Euphorbia
viguieri, E. heterophylla and E. leucocephala are the good enzyme source. Moderate activity found in Jatropha
integerrima, Jatropha multifida, Jatropha podagrica, Euphorbia pulcherrima, and Dalechampia scandens. While
different tissues of Acalypha hispida, Acalypha wilkesiana, Breynia nivosa, Cicca acida, Codiaeum variegatum, Drypetes
roxburghii are devoid of proteolytic activity. This paper describes in detail about name of plants, habitat and presence of
proteolytic enzyme in them. Results show that the out of twenty five plants 50% plant tissue synthesize protease in
appreciable amount, while 10% are not able to produce it. However 40% plants demonstrate only detectable amount of
protease. A comparative account of proteolytic activity reveals some promising plants good source of enzyme. Some
plants produce combination of cysteine and serine proteases. A single plant i.e. Euphorbia nerifolia latex contains
cysteine, serine, metallo-protease and aspartic proteases. In turn, these proteases may be used in various industrial uses in
general and cheese production Key-words- Garden Euphorb iiann a p plaarnttisc,u Clayr.s t e ine and serine protease, E. leucocephala, Euphorbia viguieri
Screening of Aspergillus oryzae M/4 for extra cellular alphaamylase productionIJERA Editor
Aspergillus oryzae M was cultivated for 42 days in submerged conditions of growth using new method of fungal cultivation. This method based on immobilizing enzymes producers on solid career in submerged conditions of growth allows to prolong the process of fungal cultivation and to maintain high enzymatic activity for a long period of time. To identify the active isolate formed on the deep fungal mycelium, a selection of a number of isolates was carried out at different periods of submerged cultivation. Aspergillus oryzae M/4 was selected as active isolate with improved extra cellular -amylase activity.
In vitro tuberization and colchicine content analysis of Gloriosa superba L.Nagendra Worlds
Gloriosa superba L. is an herbaceous climber distributed in tropical parts of the
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
DOI:10.21276/ijlssr.2016.2.4.5
ABSTRACT- Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were
the world’s largest producer and exporter of cardamom till 1980. There has however been a reduction in production,
mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed
effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from
Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised
against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA
(DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid
in using disease free material for better yields and hence increased profit to the farmer. Key-words- Cardamom mosaic virus (CdMV), Electron microscopy, Direct antigen coating Enzyme linked
immunosorbent Assay (DAC-ELISA), Dot-ELISA (DIBA)
DOI:10.21276/ijlssr.2016.2.4.6
ABSTRACT- Amylases are among the most important enzymes used in various industries. They represent
approximately 30% of the world enzyme production. These are of ubiquitous occurrence and hold the maximum market
share of enzyme sales. These comprise hydrolases, which hydrolyze starch to diverse products as dextrins, and
progressively smaller polymers composed of glucose units. They are highly demanded in various arrays such as food,
pharmaceuticals, textiles, detergents, etc. However, enzymes from mold and bacterial source have dominated applications
in industrial sectors while few species of yeast were studied for the amylases production. This review focuses on the
amylolytic yeasts and their enzymes and we were interested at α- amylase and pullulanase, their distribution,
structural-functional aspects, physical and chemical parameters, and the use of these enzymes in industrial applications. Key-words- Amylolytic yeast, α-amylase, Pullulanase, Industrial application
In vitro regeneration of multiple shoots from encapsulated somatic embryos of...IOSR Journals
A protocol has been established for plant regeneration from encapsulated somatic embryos of Artemisia vulgaris L. Encapsulated somatic embryo of A. vulgaris gave better response at 2% alginate, 75mM calcium chloride hardened for 30 minutes. Morphogenic response and percentage of conversion into plantlets of encapsulated beads on storage and temperature effect were studied. Further, encapsulated somatic embryo retrieved from storage conditions was evaluated for its viability. The encapsulated embryos can be stored upto 4 months at 40C, 200C and 220C. Maximum conversion frequency of 90% was observed from encapsulated somatic embryos cultured on MS medium supplemented with GA3 (1.5mg/l), IAA (0.5mg/l) and Ascorbic acid (40mg/l) and 22oC temperature was found to be optimum irrespective of storage periods. In the present investigation, the development of multiple shoots from a single synthetic seed is a recent record.
Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induc...Agriculture Journal IJOEAR
Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
Phosphate solubilizing potential of Rhizobium and Bacillus species for enhanc...Innspub Net
By International Network For Natural Sciences INNSPUB in Agronomy and Horticulture.
A field experiment was conducted to evaluate the separate and integrated effect of Rhizobium and Bacillus spp. on the growth of maize (Zea Mays L.). Inocula of Rhizobium and Bacillus were applied as seed coating. Recommended dose of fertilizer (120-60 kg NP ha-1) was applied at sowing. The treatments were implied according to Randomized Complete Block Design with three repeats. Inoculation had no significant effect on the leaf length (84cm) and internodal distance (18.3cm) compared to their respective control (80cm and16.5cm) but the photosynthetic rate (105.3μ mol-2s-1) , transpiration rate (13.2 mmolm-2s-1), plant height (259.3cm), leaf width (7.7cm), stem diameter (15.43mm), leaf area (644cm-2) and shoot fresh weight (79.6 tones ha-1) were significantly improved by co-inoculation. Effect of Bacillus was statistically at par with co-inoculation regarding transpiration rate (11.47 m mol m-2s-1), plant height (249.3 cm) and stem diameter (14.87 mm). Response of leaf width, stem diameter, leaf area and shoot fresh weight were significantly higher by Rhizobium application compared to the Bacillus inoculation, however, positive influence was observed by all the inoculation treatments over the control.These findings indicated that inoculation of Rhizobium and Bacillus has positive effect on the maize growth and their co-inoculation (Rhizobium+ Bacillus) showed more pronounced results. Get the full articles at: http://www.innspub.net/volume-4-number-1-january-2014-2/
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Solid state fermentation of Lathyrus sativus and sugarcane bagasse by Pleurot...Innspub Net
Lathyrus sativus (Khesari plant) and sugarcane bagasse are considered as agro wastes. Khesari plants are generally grown in fields as weeds and they have to be removed prior to cultivation. Taking this view in mind, we have investigated the conversion of these lignocellulosic agro-waste as an enriched feed stock for cattle via by solid state fermentation using a cellulolytic fungus, Pleurotus sajor-caju.The strain required 8 weeks to complete the fermentation on both the untreated and treated (with alkali, lime and presoaked) substrates at 30°C. Higher amounts of reducing sugar and soluble protein were found in each of the lime treated substrates than untreated substrates. Results also indicated that presoaked substrate contain higher amounts of reducing sugar and soluble protein than unsoaked substrate. Among the substrates, mixed substrate (khesari plant + sugarcane bagasse) was found to accumulate higher amount of sugar, 22.15 mg/g and protein, 22.80 mg/g than those of khesari plant in the 5th week of fermentation. The treatments that augmented the level of sugar and protein were also found to enhance the cellobiase, carboxy methyl cellulase and avicelase activity of crude culture extracts. These results suggest that lime treatment and presoaking seem to increase the digestibility of the substrates by the fungal cellulolytic enzymes. During eight weeks of fermentation, relatively higher cellobiase activity was found as compared to that of carboxymethylcellulase and avicelase at 30 o C for the fungul strain. The results of the present study clearly indicate that fungal conversion with pretreatment transform these lignocellulosic agro-wastes to a nutritionally enriched animal feed. Get the full articles at: http://www.innspub.net/volume-4-number-5-may-2014-2/
Economization of Datura Plant Using Planttissue Cultureiosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Welcome to International Journal of Engineering Research and Development (IJERD)IJERD Editor
call for paper 2012, hard copy of journal, research paper publishing, where to publish research paper,
journal publishing, how to publish research paper, Call For research paper, international journal, publishing a paper, IJERD, journal of science and technology, how to get a research paper published, publishing a paper, publishing of journal, publishing of research paper, reserach and review articles, IJERD Journal, How to publish your research paper, publish research paper, open access engineering journal, Engineering journal, Mathemetics journal, Physics journal, Chemistry journal, Computer Engineering, Computer Science journal, how to submit your paper, peer reviw journal, indexed journal, reserach and review articles, engineering journal, www.ijerd.com, research journals
Screening, Production and Characterization of Biosurfactants from Caatinga´S ...inventionjournals
The Caatinga biome may be a source for obtaining metabolites with unique features, such as biosurfactants of microbial origin, amphipathic molecules capable of reducing the surface tension and emulsify hydrocarbons. This study aimed to production and characterization of biosurfactants produced by Caatinga’s filamentous fungi, collected in Sumé city, stored in a collection at CDSA/UFCG. In this research, 10 filamentous fungi were evaluated in relation to emulsifier capacity, totaling six specimens (60%) presented results for Emulsification Index (EI), stability after 24 hours and Emulsification Activity (EA), with emphasis for metabolic liquids produced by fungi CDSA17, CDSA54 and CDSA71, from the genus Aspergillus, that were also evaluated under extreme conditions of temperature and pH, with higher EI at temperature of 60 °C, pH 7.0 and salt concentration 10%. In factorial design 2², in duplicate, analyzing variable of shaking (100 and 200 rpm) and glucose concentration (15 g/L and 25 g/L), tested filamentous fungi showed different behaviors and higher IE were obtained under conditions of 100 rpm and glucose concentration of 15 g/L. According to the above, it is clear that Caatinga’s filamentous fungi have potential to produce biosurfactants
Identification and Evaluation of Antifungal Compounds from Botanicals for th...researchagriculture
Red rot is a devastating disease in sugarcane caused by fungus,
Colletotrichum
falcatum
. In this study, eighteen different botanicals were screened for
identifying effective antifungal compound against
C.
falcatum.
Among the plants
screened, 15 per cent aqueous leaf extract of
Psoralea corylifolia
alone inhibited 100
per cent growth of both mycelium as well as spore germination under
in vitro
conditions. The extract did not exhibit any inhibitory effect to the beneficial microbes
viz.
,
Pseudomonas fluorescens
,
Bacillus megaterium
and
Gluconacetobacter
diazotrophicus
which are normally used in sugarcane. The effective plant extracts
exhibiting 100 per cent antifungal activity was subjected to TLC, HPLC and GC
-
MS
analysis to identify the bioactive antifungal compound. It revealed the
presence of
7H
-
furo [3,2
-
G] (1) benzopyran
-
7
-
one as main bioactive compound which is thought to be
the intermediate of antifungal compound, 8
–
methoxypsoralen formed during
biosynthesis.
Plant Tissue culture part II by Dr. Preeti VermaPreeti Verma
This presentation is meant only for educational purpose and includes various aspects of Plant Tissue culture in brief, including Media, Requirements, Problems in PTC, Techniques, Basic requirements for PTC LAB, Adavantages and Applications
DOI: 10.21276/ijlssr.2016.2.4.7
ABSTRACT- Most of the Euphorbian plants secrete fluid which contain a proteolytic enzyme for defensive role against
insects, pests and hence eco physiological inheritance to sustain vegetation eventually in adverse environmental
conditions. Evaluation has been carried out on twenty five Euphorbian garden plants for their proteolytic activities using
casein as a substrate. Out of these, Euphorbia nerifolia, Euphorbia milli, Euphorbia tirucalli, Euphorbia lactea,
Synadenium granti, Jatropha curcas, Euphorbia nivulia, Euphorbia antiquoram, Pedilanthus tithymaloides, Euphorbia
viguieri, E. heterophylla and E. leucocephala are the good enzyme source. Moderate activity found in Jatropha
integerrima, Jatropha multifida, Jatropha podagrica, Euphorbia pulcherrima, and Dalechampia scandens. While
different tissues of Acalypha hispida, Acalypha wilkesiana, Breynia nivosa, Cicca acida, Codiaeum variegatum, Drypetes
roxburghii are devoid of proteolytic activity. This paper describes in detail about name of plants, habitat and presence of
proteolytic enzyme in them. Results show that the out of twenty five plants 50% plant tissue synthesize protease in
appreciable amount, while 10% are not able to produce it. However 40% plants demonstrate only detectable amount of
protease. A comparative account of proteolytic activity reveals some promising plants good source of enzyme. Some
plants produce combination of cysteine and serine proteases. A single plant i.e. Euphorbia nerifolia latex contains
cysteine, serine, metallo-protease and aspartic proteases. In turn, these proteases may be used in various industrial uses in
general and cheese production Key-words- Garden Euphorb iiann a p plaarnttisc,u Clayr.s t e ine and serine protease, E. leucocephala, Euphorbia viguieri
Screening of Aspergillus oryzae M/4 for extra cellular alphaamylase productionIJERA Editor
Aspergillus oryzae M was cultivated for 42 days in submerged conditions of growth using new method of fungal cultivation. This method based on immobilizing enzymes producers on solid career in submerged conditions of growth allows to prolong the process of fungal cultivation and to maintain high enzymatic activity for a long period of time. To identify the active isolate formed on the deep fungal mycelium, a selection of a number of isolates was carried out at different periods of submerged cultivation. Aspergillus oryzae M/4 was selected as active isolate with improved extra cellular -amylase activity.
In vitro tuberization and colchicine content analysis of Gloriosa superba L.Nagendra Worlds
Gloriosa superba L. is an herbaceous climber distributed in tropical parts of the
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
DOI:10.21276/ijlssr.2016.2.4.5
ABSTRACT- Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were
the world’s largest producer and exporter of cardamom till 1980. There has however been a reduction in production,
mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed
effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from
Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised
against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA
(DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid
in using disease free material for better yields and hence increased profit to the farmer. Key-words- Cardamom mosaic virus (CdMV), Electron microscopy, Direct antigen coating Enzyme linked
immunosorbent Assay (DAC-ELISA), Dot-ELISA (DIBA)
DOI:10.21276/ijlssr.2016.2.4.6
ABSTRACT- Amylases are among the most important enzymes used in various industries. They represent
approximately 30% of the world enzyme production. These are of ubiquitous occurrence and hold the maximum market
share of enzyme sales. These comprise hydrolases, which hydrolyze starch to diverse products as dextrins, and
progressively smaller polymers composed of glucose units. They are highly demanded in various arrays such as food,
pharmaceuticals, textiles, detergents, etc. However, enzymes from mold and bacterial source have dominated applications
in industrial sectors while few species of yeast were studied for the amylases production. This review focuses on the
amylolytic yeasts and their enzymes and we were interested at α- amylase and pullulanase, their distribution,
structural-functional aspects, physical and chemical parameters, and the use of these enzymes in industrial applications. Key-words- Amylolytic yeast, α-amylase, Pullulanase, Industrial application
In vitro regeneration of multiple shoots from encapsulated somatic embryos of...IOSR Journals
A protocol has been established for plant regeneration from encapsulated somatic embryos of Artemisia vulgaris L. Encapsulated somatic embryo of A. vulgaris gave better response at 2% alginate, 75mM calcium chloride hardened for 30 minutes. Morphogenic response and percentage of conversion into plantlets of encapsulated beads on storage and temperature effect were studied. Further, encapsulated somatic embryo retrieved from storage conditions was evaluated for its viability. The encapsulated embryos can be stored upto 4 months at 40C, 200C and 220C. Maximum conversion frequency of 90% was observed from encapsulated somatic embryos cultured on MS medium supplemented with GA3 (1.5mg/l), IAA (0.5mg/l) and Ascorbic acid (40mg/l) and 22oC temperature was found to be optimum irrespective of storage periods. In the present investigation, the development of multiple shoots from a single synthetic seed is a recent record.
Influence of Plant Growth Regulators and Explant Type on Multiple Shoot Induc...Agriculture Journal IJOEAR
Abstract— Sesamum indicum L. is used as an important oil crop in the world. For establishing of a simple and a rapid system for in vitro culture of sesame shoot tips and hypocotyls explants were cultured on MS media with differtent combinations and concentrations of PGRs. On medium with 5 mgl-1BA plus 1 mgl-1 IAA and 1 mgl-1 ABA mutliple shoot induction on explants was occurred. Multiple shoot indution on medium supplemented with 2 mgl-1BA and 0.3 mgl-1 NAA happen with a short phase of callusing. Also MS medium with 3 mgl-1 2,4-D and 0.5 mgl-1 kinetin was a suitable medium for inducing of somatic embryogenic calli with the frequency of sub-culturing potential. Regenerated shoots on both media rooted on MS medium plus 1.5 mgl-1 NAA and 0.03 mgl-1 BA and whole regenerated plantlets formed.
Phosphate solubilizing potential of Rhizobium and Bacillus species for enhanc...Innspub Net
By International Network For Natural Sciences INNSPUB in Agronomy and Horticulture.
A field experiment was conducted to evaluate the separate and integrated effect of Rhizobium and Bacillus spp. on the growth of maize (Zea Mays L.). Inocula of Rhizobium and Bacillus were applied as seed coating. Recommended dose of fertilizer (120-60 kg NP ha-1) was applied at sowing. The treatments were implied according to Randomized Complete Block Design with three repeats. Inoculation had no significant effect on the leaf length (84cm) and internodal distance (18.3cm) compared to their respective control (80cm and16.5cm) but the photosynthetic rate (105.3μ mol-2s-1) , transpiration rate (13.2 mmolm-2s-1), plant height (259.3cm), leaf width (7.7cm), stem diameter (15.43mm), leaf area (644cm-2) and shoot fresh weight (79.6 tones ha-1) were significantly improved by co-inoculation. Effect of Bacillus was statistically at par with co-inoculation regarding transpiration rate (11.47 m mol m-2s-1), plant height (249.3 cm) and stem diameter (14.87 mm). Response of leaf width, stem diameter, leaf area and shoot fresh weight were significantly higher by Rhizobium application compared to the Bacillus inoculation, however, positive influence was observed by all the inoculation treatments over the control.These findings indicated that inoculation of Rhizobium and Bacillus has positive effect on the maize growth and their co-inoculation (Rhizobium+ Bacillus) showed more pronounced results. Get the full articles at: http://www.innspub.net/volume-4-number-1-january-2014-2/
A Study on the Removal of Pesticide Residues on Potatoes Using Moringa oleife...AI Publications
Moringa oleifera (MO) is a plant whose seeds possess strong coagulation properties capable of removing turbidity and heavy metals from water. In this study, the effectiveness of MO seeds as a potential pesticide removal apparatus for agricultural produce was investigated. The parameters of interest for each pesticide washing solution were pesticide-concentration reduction, cost, and taste alteration to the potatoes. Separate doses of 0.25 mL profenofos 500 EC were individually applied to distinct 250 g pesticide-free potato piles. A submersion of each tainted potato pile was carried out in one of the chosen pesticide baths (mineral water, 2% salt solution, 10% salt solution, vegetable bath wash, and 10 g MO seed) for 10 min, followed by a 10 second mineral water bath rinse. Remaining profenofos residue concentrations on potatoes were quantified using the quick, easy, cheap, rugged, and simple QuEChERs method coincided with High Performance Liquid Chromatography (HPLC). The experiments showed that the MO seed was the most effective at removing profenofos at 52.9% followed by the vegetable bath at 47.3%. Neither method caused an alteration in food taste, but the MO seed method is significantly cheaper at 2,000/L IDR ($0.14 USD) compared to the vegetable bath at IDR 10,668/L ($0.77 USD) at the time of the experiment, respectively.
Solid state fermentation of Lathyrus sativus and sugarcane bagasse by Pleurot...Innspub Net
Lathyrus sativus (Khesari plant) and sugarcane bagasse are considered as agro wastes. Khesari plants are generally grown in fields as weeds and they have to be removed prior to cultivation. Taking this view in mind, we have investigated the conversion of these lignocellulosic agro-waste as an enriched feed stock for cattle via by solid state fermentation using a cellulolytic fungus, Pleurotus sajor-caju.The strain required 8 weeks to complete the fermentation on both the untreated and treated (with alkali, lime and presoaked) substrates at 30°C. Higher amounts of reducing sugar and soluble protein were found in each of the lime treated substrates than untreated substrates. Results also indicated that presoaked substrate contain higher amounts of reducing sugar and soluble protein than unsoaked substrate. Among the substrates, mixed substrate (khesari plant + sugarcane bagasse) was found to accumulate higher amount of sugar, 22.15 mg/g and protein, 22.80 mg/g than those of khesari plant in the 5th week of fermentation. The treatments that augmented the level of sugar and protein were also found to enhance the cellobiase, carboxy methyl cellulase and avicelase activity of crude culture extracts. These results suggest that lime treatment and presoaking seem to increase the digestibility of the substrates by the fungal cellulolytic enzymes. During eight weeks of fermentation, relatively higher cellobiase activity was found as compared to that of carboxymethylcellulase and avicelase at 30 o C for the fungul strain. The results of the present study clearly indicate that fungal conversion with pretreatment transform these lignocellulosic agro-wastes to a nutritionally enriched animal feed. Get the full articles at: http://www.innspub.net/volume-4-number-5-may-2014-2/
Economization of Datura Plant Using Planttissue Cultureiosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Welcome to International Journal of Engineering Research and Development (IJERD)IJERD Editor
call for paper 2012, hard copy of journal, research paper publishing, where to publish research paper,
journal publishing, how to publish research paper, Call For research paper, international journal, publishing a paper, IJERD, journal of science and technology, how to get a research paper published, publishing a paper, publishing of journal, publishing of research paper, reserach and review articles, IJERD Journal, How to publish your research paper, publish research paper, open access engineering journal, Engineering journal, Mathemetics journal, Physics journal, Chemistry journal, Computer Engineering, Computer Science journal, how to submit your paper, peer reviw journal, indexed journal, reserach and review articles, engineering journal, www.ijerd.com, research journals
Screening, Production and Characterization of Biosurfactants from Caatinga´S ...inventionjournals
The Caatinga biome may be a source for obtaining metabolites with unique features, such as biosurfactants of microbial origin, amphipathic molecules capable of reducing the surface tension and emulsify hydrocarbons. This study aimed to production and characterization of biosurfactants produced by Caatinga’s filamentous fungi, collected in Sumé city, stored in a collection at CDSA/UFCG. In this research, 10 filamentous fungi were evaluated in relation to emulsifier capacity, totaling six specimens (60%) presented results for Emulsification Index (EI), stability after 24 hours and Emulsification Activity (EA), with emphasis for metabolic liquids produced by fungi CDSA17, CDSA54 and CDSA71, from the genus Aspergillus, that were also evaluated under extreme conditions of temperature and pH, with higher EI at temperature of 60 °C, pH 7.0 and salt concentration 10%. In factorial design 2², in duplicate, analyzing variable of shaking (100 and 200 rpm) and glucose concentration (15 g/L and 25 g/L), tested filamentous fungi showed different behaviors and higher IE were obtained under conditions of 100 rpm and glucose concentration of 15 g/L. According to the above, it is clear that Caatinga’s filamentous fungi have potential to produce biosurfactants
Isolation and partial characterization of a new strain of Klebsiella pneumoni...GJESM Publication
Glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 PDO). Here we report a newly isolated bacterial strain BA11 from soil, capable of fermenting glycerol to 1,3 PDO, and
has been identified to be a strain of Klebsiella pneumoniae. Strain BA11 was fast growing showing peak 1,3 PDO production in 6 h of cultivation with productivity of 1.2 g/L-h without the addition of Vitamin B12. Based on the
optimum glycerol utilization (75%) and 1,3 PDO production (8.3 g/L) and yield (0.56 mol/mol glycerol utilized), the
most appropriate glycerol concentration for cultivation was 20 g/L. The strain BA11 could tolerate the pH range of 6
to 8.5 as no inhibitory effects were seen on growth as well as 1,3 PDO production. Strain BA11 was most active and
could produce high 1,3 PDO in the incubation temperature range of 25 to 40 oC. The production of 1,3 PDO was
maximum (9.3 g/L) under aerobic condition with 95.8% glycerol utilization. Addition of glucose to the glycerol fermentation led to increased cell mass but no improvement in the 1,3 PDO production.
Studies on the viabile bacteria of commercial probiotic products available in...Premier Publishers
The viability of bacteria in seven probiotic products for animal production available in Bangladesh namely Bactosac, Micro guard, Probac, Poultry Star sol, Gutpro, Clostat 11 and Rumilac were tested. All the products were purchased in local markets. The bacteria in the probiotic product were grown anaerobically using Luria-Bertani (LB) broth and incubated for 13 h at 37° C. The viable bacteria of commercial probiotics ranged between 6.8 ×102 to 2.0×104 cfu/g. The highest values (2.0×104 cfu/g) were found in Microguard and Probac and the lowest value (6.8 ×102) was found in Gutpro. However, viable cells in Microguard and Probac were found lower by four and three logarithmic cycles, respectively, than manufacturer statements (5.0×108/g and 3.0×107/g). The viable cells found in the probiotic products were not accepted as the minimum level of 106 cfu/ml or cfu/g. The results of the present study concluded that viability of bacteria in commercial probiotic products were not found at a minimum level and therefore may not be sufficient for colonization of the animal gut.
Effect of Different Physico-Chemical Parameters on Production ofAmylase by Ba...IOSR Journals
The present study is concerned with the production of amylase by Bacillus species strain. In this
study 12 bacterial strains were isolated and screened for their α-amylase activity. These strains were
maintained on nutrient agar medium. Fermentation for the production of amylase was carried out in the enzyme
production medium (EPM). All the 12 strains were tested for amylase production. On the basis of maximum
amylase activity strain no.1 was selected for further studies. Different starch concentrations, 0.75,1.00,1.25%,
pH labels 6.5,7.0,7.5,8.0, aeration (RPM), 100,120,140, temperatures 250C,280C,370C, and 400C and inoculums
level 0.5%,1.0%, 1.5% and 2.0% were studied
Study on Culture Conditions for A Cellulase Production From As Pergillus UnguisIJMERJOURNAL
ABSTRACT: Cellulase is a common name of enzymes which catalyze cellulolysis. Specially,cellulase is widely used in food processing, animal feed, chemicals, textile,fuel and pollution treatment.The objective of this research is to study on optimal conditions for the production of cellulase byAspergillus unguis. The study was designed as a comparative culture conditions such as carbon sources, moisture content, duration, nitrogen sources and citrate buffer content on cellulase production for Aspergillus unguis. Cellulase activity was determined by measuring the absorbance at λ = 540 nm with 3,5-DNS reagent. In optimized culture conditions, enzyme activity of Aspergillus unguis achieved 110.92U/ml in comparison with a commercial cellulase with 185.33U/ml in enzyme activity. The value of cellulase activity of Aspergillus unguis is 41% lower than commercial enzymes. However, enzyme in this study was raw enzyme and the cost of producing1 litter of this enzyme is just 1/8 that of purified ones. The enzyme activity would be increased by purification. That fact has proven the applicability of using the findings of this study to improve cellulase production.
Influence of phosphorous acid application on the accumulation of total phenol...Innspub Net
One mechanism used by coconut plant to protect itself against Phytophthorakatsurae is linked to total
polyphenols production. This study aimed to investigate the impact of phosphorous acid plant treatment on the
production of total polyphenols in coconuthusk, as part of chemical control.The study was conducted on two
coconuts cultivars (EGD and PB 121+) with four doses of phosphorous acid [Control, 2.8 g (TA), 5.6 g (TB), 11.2 g
(TC)]. At each sampling, the husks were processed and extracts were prepared for total polyphenols assays. There
was significant difference between EGD and PB 121+ total polyphenols production (p<0.001).The interaction
between coconut variety and phosphorous acid doses was also significant.The interaction EGD and TC had the
highest total polyphenols accumulation of 4838.5 µg/g of fresh weight (FW). For PB121+, the highest total polyphenols accumulation of 6433.71 µg/g FW was obtained from the interaction between PB121+ and T0. From this observation, it could be statedthat phosphorous acid only triggers the treated plantdefense mechanisms to produce total phenolic compounds when attacked by a pathogen. Get more articles at: http://www.innspub.net/volume-7-number-3-september-2015-ijaar/
Optimum Conditions for Alginaseby Bacilllus Circulans R Isolateiosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Water treatment using moringa oleifera seeds and kernals in stream watereSAT Publishing House
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
Antimicrobial activity of Lactobacillus spp. especially (L. planetarium and L. acidophilus) against S. aureus were tested using agar-plug, agar well diffusion methods to select the best isolate that could inhibit the growth of multidrug resistance isolates. Further identification for the presence of bacteriocin was done using ELISA kit. Results showed that Lactobacillus spp isolates were bacteriocin producers with different degrees and that L. planetarium (L7) was the most efficient in bacteriocin production. Therefore, L. planetarium (L7) was selected for purification using 70% saturated ammonium sulfate and gel chromatography. The effect of purified bacteriocin was tested on 16 bacterial isolates using micro-titer plate method and well diffusion method. The results showed the ability of the bacteriocin to inhibit bacteria only at concentrations 1866U/ml (50%), 3732U/ml (100%) with a diameter of inhibition zones ranges between (11-23 mm) respectively. The anti-biofilm activity of purified bacteriocin at concentration 100% was investigated and the results showed that biofilm formation was reduced by 100% in the presence of bacteriocin.
To conserve endangered medicinal plant species, Exacum bicolor Roxb.
(Gentianaceae), an efficient micropropagation protocol was developed by using nodal
segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in
vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/ l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be
successfully acclimatized at a rate of 80 % survivability.
To conserve endangered medicinal plant species, Exacum bicolor Roxb. (Gentianaceae), an efficient micropropagation protocol was developed by using nodal segments for axillary shoot proliferation. Higher multiplication rate was achieved with 78% on MS medium supplemented with growth hormones, IBA and GA3 at 1.5 mg/l and 0.5 mg/l respectively. Rooting was more effective with 75% of the node derived in vitro shoots in MS basal medium with the auxins, IBA and NAA at 1.0 mg/l and 0.5 mg/l respectively. Using the hardening medium containing garden soil, sand and vermiculate in the ratio of 1:1:1 by volume, regenerated plantlets could be successfully acclimatized at a rate of 80 % survivability.
A comparative study of procedures for binding of aflatoxin M1 to 1 Lactobacil...Jean Claude Assaf
Several strains of Lactic Acid Bacteria (LAB), frequently used in food fermentation and 20 preservation, have been reported to bind different types of toxins in liquid media. This study 21 was carried out to investigate the effect of different concentrations of Lactobacillus 22 rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding 23 was tested following repetitive washes or filtration procedures in combination with additional 24 treatments as heat, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 25 was incubated for 18 hours at 37 °C and the unbound AFM1 was quantified by HPLC. The 26 stability of the bacteria-AFM1 complex for both viable and heat treated bacteria was tested. 27 Depending on the bacterial concentration and procedure used, the percentages of bound AFM1 28 by L. rhamnosus GG varied from as low as undetectable to as high as 63%. The highest 29 reduction in the level of unbound AFM1 was recorded for the five washes procedure that 30 involved heat and pipetting treatments. Results also showed that binding was partially 31 reversible and AFM1 was released after repeated washes. These findings highlight the effect 32 of different treatments on the binding of L. rhamnosus GG to AFM1 in liquid matrix.
Analysis of Artificial Intelligence Techniques for Network Intrusion Detectio...IIJSRJournal
With the rapid advancement of computer technology during the last couple of decades. Computer systems are commonly used in manufacturing, corporate, as well as other aspects of human living. As a result, constructing dependable infrastructures is a major challenge for IT managers. On the contrary side, this same rapid advancement of technology has created numerous difficulties in building reliable networks which are challenging tasks. There seem to be numerous varieties of attacks that affect the accessibility, authenticity, as well as secrecy of communications systems. In this paper, an in-depth and all-inclusive description of artificial intelligence methods used for the detection of network intrusions is discussed in detail.
Methodologies for Enhancing Data Integrity and Security in Distributed Cloud ...IIJSRJournal
Usually, cloud infrastructure is used individually by businesses, whereas the hybrid cloud would be a blend of two or many kinds of clouds. Because as clouds become increasingly common, safety issues also expanding. Because of such cybersecurity threats, numerous experts suggested procedures as well as ways to assure internet confidentiality. Providers of cloud-based services were accountable for the complete safety of cloud information. Nevertheless, since the clouds are accessible (easily accessible over the World wide web), much research has been conducted on cloud storage cybersecurity. This paper describes methods for enhancing security and reliability in decentralized cloud-based solutions, as well as suggests a few security solution methods of implementation.
Agriculture in Indian Economy and Contribution of Science and Technology IIJSRJournal
One of the oldest occupations in history, agriculture has benefited much from innovation throughout the years. Since then, science has played a significant role in agricultural innovation and quality assurance. We have listed a few of the factors that were mentioned in the introduction section if you'd want to understand more about the significance of science and technology in agriculture. Encouraging the use of science and technology is the cornerstone for improving agriculture's productivity, quality, efficiency, and competitiveness, which also contributes to the modernization of agriculture and rural areas, ensures food security, social security, and increases the income of agricultural producers and traders.
The Effect of Kronecker Tensor Product Values on ECG Rates: A Study on Savitz...IIJSRJournal
This article presents a study on ECG signal filtering algorithms to denoise signals corrupted by various types of noise sources. The study also examines the effect of Kronecker tensor product values on ECG rates. The study is conducted in a Matlab environment, and the results demonstrate that a constant number for the respective codes can effectively denoise ECG signals without any trouble. These findings have significant implications for diagnosing abnormal heart rhythms and investigating chest pains. The present study is novel in that it explores the relationship between ECG rate and Kronecker delta values across different age groups, which has not been extensively studied in previous literature. The study's unique contribution is the determination of age-specific values of the constant K required to represent this relationship accurately in different populations, which could inform the development of more effective algorithms for denoising ECG signals in clinical settings. Additionally, this study's finding of an inverse relationship between ECG rate and Kronecker delta values could have broader implications for understanding the physiological factors that contribute to variability in ECG measurements. The study provides valuable insights into ECG signal processing and suggests that the implemented techniques can improve the accuracy of ECG signal analysis in real-time clinical settings. Overall, the manuscript is a valuable contribution to the field of biomedical signal processing and provides important information for researchers and healthcare professionals.
Basic Criteria for Building the Third Renaissance in Uzbekistan IIJSRJournal
On the occasion of the 29th anniversary of the independence of the Republic of Uzbekistan, President Shavkat Mirziyoyev emphasized that the goal of our development should be the Third Renaissance. This strategic idea, in its grandeur, shows the need to aim for a common goal in all aspects of national development. In practice, the head of state expressed the new and clarified content of the national idea of Uzbekistan at the current stage of development. This article analyzes the important role of literature, theater and art in establishing the Third Renaissance in Uzbekistan.
Assessment of Neglected and Under-Utilized Crop Species of African Horned Mel...IIJSRJournal
There is an increasing interest in neglected and under-utilized crop species (NUS) throughout the world, reflecting a growing trend within agriculture to identify and develop new crops for export and domestic markets. Interest in NUS stems from a variety of factors, including their contribution to agricultural diversification and better use of land, their economic potential and the opportunities they provide for diet diversification. The main objective was to assess the economic and nutritional value of neglected and utilized crop species of African horned melon in Zambia.
The study used the qualitative research design and descriptive, using desk review to collect secondary data from various literature on neglected and under-utilized crops species of African horned melon.
In conclusion, the findings reveal that the African horned melon has nutritional value consisting numerous vitamins and antioxidants which are beneficial to health living of humans and will contribute and broaden food diversity and nutritional among rural and urban communities of Zambia. The crop will promote healthy living among Zambian citizen to overcome malnutrition and obesity. Further, African horned melon is a climate change crop that will enhancing rural resiliency and climate smart agriculture activities in many areas. Communities must be trained and have knowledge experience of farmer-to-farmer capacity building in rural areas of Zambia.
Prevalence of Trichomonas Vaginalis Infection Among Married Pregnant Women in...IIJSRJournal
A cross-sectional survey of Trichomonas vaginalis infection has been conducted among married pregnant women attending antenatal clinics, for the first time in pregnancy, the direct microscopy technique was adopted. Of the 120 pregnant women studied, 4(3.3%) were infected with T. vaginalis. Individuals age 20-25 years were most infected (3.7%). Women in their third trimester of pregnancy were significantly more infected (1.1%), than those in their second trimester (1.6%) and first trimester (2.3%). Despite reporting a low prevalence of T. vaginalis among pregnant women in the study, this does not imply completely ruling out the presence of T. vaginalis among pregnant women due to the diagnostic technique and also that even the low occurrence among pregnant women in the hospitals cannot totally explain general occurrence. T. vaginalis infection can be dangerous and poses serious threat to the health. Hence, the need for prevention of T. vaginalis and that efforts for prevention of T. vaginalis infection should be targeted at all women of child bearing age. Since T. vaginalis is primarily sexually transmitted, educational efforts must be aimed at high risk groups including women without any formal education and must be explicit regarding the behaviours that leads to the spread of T. vaginalis, and other sexually transmitted infections. There is also the need for proper counseling and education on sexual behaviour and genital hygiene which would greatly help in the prevention of the infection.
Factors Influencing Professional Project Management Ethical Practices in Buil...IIJSRJournal
Ethical practices are essential to providing quality work which cut across every sector. In building construction, adherence to project management ethical standards is essential to providing quality services that can stand the test of time. However, many building projects have been constructed with standards that are far below the professional ethics. This is evident in the cases of building failure reported throughout the country. The study examines the factors that influences project management ethical practices in Nigeria and specifically in Lagos. A total of 384 samples were selected from project stakeholders and construction professionals. A well-structured 25 items questionnaire was designed to elicit for response on ethical practices and factors that influences ethical practices. The results indicated that ethical practices in project management are influenced by various factors, including the project environment and stakeholder's impact as organizational factors. The major project-related factors that affect ethical practices are project scope and complexity, project financing, project risk, and project stakeholders, while project managers' technical skills, qualifications, and personal values have significant impacts on adherence to ethical practices. The influencing factors could be related to the organization, the project, or the professional, but in most cases, they are a combination of these factors. Therefore, it is recommended that thorough assessments are conducted before, during, and after construction, and different professionals should be assigned to ensure transparency and compliance with standards.
Assessment of Water Occupancy Rates of the Çamlıgöze Dam Lake between 2010-20...IIJSRJournal
With the increasing world population, the importance of dam lakes is increasing within the framework of more effective and efficient use of water resources. This study focuses on the water occupancy rates of Çamlıgöze Dam Lake, located in Turkey, between the years 2010-2021. The annual average water occupancy rate of Çamlıgöze Dam Lake between 2010-2021 was calculated as 69.55 percent. This shows that approximately seventy percent of Çamlıgöze Dam Lake was full between 2010-2021. According to these values, it was determined that the water occupancy rates of Çamlıgöze Dam Lake did not face a serious decrease between 2010-2021. As a result, there is no short term problem in terms of water occupancy rates in Çamlıgöze Dam Lake, but this does not mean that it will not be a problem in the long term. For this reason, it should not be abandoned to use the water of Çamlıgöze Dam Lake effectively, economically and consciously.
Sustainability of Pod Yields of Groundnut through Crop Seasonal Rainfall, Len...IIJSRJournal
A study was conducted with the objective of assessing the effect of crop seasonal rainfall and length of growing period on the sustainability of pod yields of groundnut attained in 31 mandals under arid Alfisols of Anantapur in Andhra Pradesh. We have considered the variability of mandals with regard to (i) crop seasonal rainfall (mm) and (ii) pod yield of groundnut (kg/ha) during 2001 to 2020; (iii) extent of crop area (ha) during 2009 to 2020; and (iv) length of growing period (days). Based on the mean and standard deviation (SD) of each parameter, the mandals were classified into 5 groups viz., (i) G1: Less than (Mean–2SD); (ii) G2: (Mean–2SD) to (Mean–SD); (iii) G3: (Mean–SD) to (Mean+SD); (iv) G4: (Mean+SD) to (Mean+2SD); and (v) G5: More than (Mean+2SD). Out of 31 mandals, 22 mandals for area and crop seasonal rainfall, 20 mandals for LGP and 18 mandals for yield have fallen in G3. Estimates of correlation were derived between groundnut area, crop seasonal rainfall and yield for each mandal over years and tested for significance to assess the superiority of mandals. Significant correlation of yield and crop seasonal rainfall was observed which ranged from 0.433 at Kalyandurg to 0.765 at Putlur. Similarly, significant correlation between yield and area of groundnut was observed in Kalyandurg (-0.764), Brahmasamudram (-0.674) and Rapthadu (-0.584) mandals. The predictability of yield and prediction error were derived based on a regression model of yield calibrated through the crop seasonal rainfall, LGP and crop area in different mandals. The model gave significant predictability (R2) value of 0.46 with prediction error of 90.9 kg/ha and indicated negative effect of area, positive effect of crop seasonal rainfall and LGP on yield. The sustainability yield index ranged from 26.6% (Kambadur) to 87.5% (Peddavadagur) with mean of 53.9% (CV of 30.1%) over years. Ranks were assigned to the mean and variation of area, crop seasonal rainfall, yield, LGP and SYI of each mandal and rank sums were derived. Guntakal, Gooty and Vidapanakal were superior with rank sums of 30, 38 and 70 respectively. Guntakal was superior with an area of 16570 ha (CV of 17.3%), crop seasonal rainfall of 436.1 mm (CV of 33.4%), LGP of 140 days, yield of 644 kg/ha (CV of 70.9%) and SYI of 76.5%, while Gooty was superior with area of 14146 ha (CV of 14.6%), crop seasonal rainfall of 429.6 mm (CV of 42.4%), LGP of 140 days, yield of 663 kg/ha (CV of 69.1%) and SYI of 79.1%. Similarly, Vidapanakal was superior with area of 5077 ha (CV of 31.1%), crop seasonal rainfall of 403.2 mm (CV of 47.4%), LGP of 140 days, yield of 654 kg/ha (CV of 49.5%) and SYI of 77.9%. Due to maximum LGP and crop seasonal rainfall, we recommend that the farmers of these mandals could enhance the area of groundnut and attain maximum sustainable yields under arid Alfisols.
On the Modulation of Biocompatibility of Hydrogels with Collagen and Guar Gum...IIJSRJournal
In this work, we report the synthesis of molybdenum metal-organic frameworks (Mo-MOFs) using 1,3,5-benzenetricarboxylic acid and the amino acids L-phenylalanine, L- tryptophan, and L-histidine as ligands. They were incorporated in hydrogel matrixes comprised of collagen and guar gum to obtain composite hydrogels. The effect of chemical structure of Mo-MOFs on the structure, physicochemical properties and in vitro biocompatibility of hydrogels was studied. These biomaterials showed a super absorbent performance (higher than 2000 ± 169%) and a high degree of reticulation (higher than 75 ± 6%). The microstructure of the composites showed a granular morphology with some porosity. These composites were degraded entirely by hydrolysis at pH 5 and pH 7 at room temperature in time lapses shorter than 15 days. Also, they were biocompatible with porcine dermis fibroblasts not showing cytotoxic effects up to 48 h of incubation allowing its proliferation, and it was observed that the MOF containing L-tryptophan improved notably the biocompatibility of the collagen/guar gum matrix. Finally, the matrixes were tested as vehicles for cell encapsulation and release. The slow-release rates show that fibroblasts tend to remain inside the hydrogel matrixes. Thus, these materials are more suitable for cell scaffolds and tissue engineering applications such as wound healing dressings.
Incorporation of Se (IV) Complexes based on Amino Acids in Biomatrixes in Hyd...IIJSRJournal
Selenium is a non-metal that shows biological interest since it is responsible for modulating various proteins at the micronutrient level in living beings. In this work, new complexes based on the Se (IV) ion with amino acids such as phenylalanine (Se-F), histidine (Se-H) and tryptophan (Se-T) were hydrothermally synthesized and characterized. These were incorporated into biomatrixes based on semi-interpenetrated polymeric networks (Semi-IPN) of collagen-polyurethane-guar gum (CPGG) by the microemulsion process using a mass ratio of 1 wt.% with respect to collagen. The structural and crystalline characteristics that the selenium-amino acid complexes show a performance in modulating the properties of the biomatrixes under study. The results indicate that the incorporation of the complex decreases the crosslinking of the hydrogel, generating granular surfaces with porosity dependent on the type of amino acid. The CPGG Se-T biomatrix shows a swelling capacity of 10200 ± 1100 higher than the CPGG base matrix; while the CPGG Se-F and CPGG Se-T biomatrixes present slow degradation at both physiological and acidic pH. Interestingly, the matrix that includes the Se-F complex significantly stimulates the metabolic activity of L929 fibroblasts for up to 48 h, stimulating their proliferation. The fibroblasts encapsulated on these novel biomatrixes show recurrent release capacity for up to 7 days, where the structure of the CPGG Se-H biomatrix exhibits greater release from the encapsulated cells. These results demonstrate that these innovative biomatrixes could be used in biomedical applications such as dermal tissue regeneration and cell release for a specific biological fate.
Machine Learning Based House Price Prediction Using Modified Extreme Boosting IIJSRJournal
In recent years, machine learning has become increasingly important in everyday voice commands and predictions. Instead, it provides a safer auto system and better customer assistance. As a result of all that has been demonstrated, ML is a technology that is becoming more and more popular in a range of industries. To gauge changes in house values, the House Price Index is frequently employed (HPI). Due to the substantial correlation that exists between property prices and other variables, such as location, region, and population, the HPI on its own is not sufficient to accurately forecast a person's house price. Some studies have successfully predicted house prices using conventional machine learning techniques, but they seldom evaluate the efficacy of different models and ignore the more complicated but less well-known models. We proposed Modified Extreme Gradient Boosting as our model in this study due to its adaptive and probabilistic model selection process. Feature engineering, hyperparameter training and optimization, model interpretation, and model selection and evaluation are all steps in the process. Home price indices, which are frequently used to support real estate policy initiatives and estimate housing costs. In this project, models for forecasting changes in home prices are developed using machine learning methods.
Preliminary Evaluation on Vegetative of Rambutan (Nephelium lappaceum) in San...IIJSRJournal
The study was initiated to evaluate the early performance of rambutan (Nephelium lappaceum) vegetative planted on marginal sandy tin-tailing soil. The experiment was carried out for one year in a plot of 4-year-old rambutan cultivar at MARDI Kundang, Rawang, Selangor, Malaysia. Varieties of Mutiara Merah and Mutiara Wangi were used. Data from the plants as a measurement of vegetative growth was recorded. Mutiara Merah proved that it can be well-grown and cultivated on sandy tin-tailing soil. The plant height of Mutiara Merah indicated the highest significant reading. The parameter of canopy width showed the same variety contributed to the highest record. Nevertheless, Mutiara Merah contributed to the highest significant reading on stem diameter and perimeter respectively. Chlorophyll content in leaves of the plant of the same variety recorded the highest SPAD reading. Further field evaluations are needed to determine the relationship of fertilizer level with the different varieties in inducing the growth and yield of rambutan planted in marginal soil.
Analysis of Physicochemical and Microbiological Parameters of Wine Produced f...IIJSRJournal
Wine is a fermented drink made by the controlled culture of yeasts on fruit juices. This study was undertaken to produce acceptable wines from blends of banana and pineapple by the fermentative action of Meyerozyma guilliermondii strain 1621 and Pichia guilliermondii strain PAX-PAT 18S. The fermentation process lasted for a period of 28 days and, the aging process was for 2 months. The fermentation process comprised two set ups- one was fermented by Meyerozyma guilliermondii strain 1621 and the other was fermented by Pichia guilliermondii strain PAX-PAT 18S. The process was monitored and controlled by carrying out physicochemical analysis (pH, temperature, specific gravity, total titratable acidity, and alcohol content) and yeast count using standard methods. There was a decrease in the pH for both wines and an increase in the total titratable acidity. The temperature was between 17 and 27 0C for both wines. The specific gravity of the wines decreased during the fermentation leading to an increase in alcohol production. There was an increase in yeast count from 6.7×107 sfu/ml to 1.8×108 sfu/ml between days 1 and 17 and a decrease from 1.8×108 sfu/ml to 0 sfu/ml between days 17 to 85 for Meyerozyma guilliermondii; also an increase from 5.1×107 sfu/ml to 1.7×108 sfu/ml from day 1 to 17, and a decrease from 1.7×108 sfu/ml to 0 sfu/ml between day 17 to 85 for Pichia guilliermondii. Statistically, there was no significant difference between the yeast counts, temperature, pH, total titratable acidity, and specific gravity but there was signa ificant difference between the alcohol production for both wines. This study shows that wines can be successfully produced using Meyerozyma guilliermondii strain 1621 and Pichia guilliermondii strain PAX-PAT 18S.
Cohesive and Thermal Properties of Sodium Cyanide-Halide Mixed Crystals IIJSRJournal
In order to analyse the cohesive and thermal properties of sodium cyanide-halide mixed crystals an Extended Three Body Force Shell Model (ETSM) has been applied by incorporating the effect of translational-rotational (TR) coupling. We have conducted theoretical research on cohesive and thermal properties, such as cohesive energy (, molecular force constant (f), compressibility (), Restrahlen frequency (, Debye temperature (D), Gruneisen parameter (), Moelwyn Hughes constants (F1) and the ratio of volume thermal expansion coefficient (v) to volume specific heat (Cv), as a function of temperature within the temperature range 50K T 300K at concentration x=0, 0.27, 0.58 and 1. The current model computations and the findings of the available experiments are in good agreement. The ETSM is a sufficiently realistic model and may be applied to a variety of other mixed crystals in this family.
Discussion on Analysis of Effects of Short-Form Video Advertising on the Purc...IIJSRJournal
The purpose of this study is to investigate the impact of informativeness, entertainment, credibility, social interaction, incentives, and irritation of short-form video advertising on social media on the purchase intention of Gen Z in Vietnam through user attitude and advertising value. The methodology is conducting a survey by collecting responses from 1257 respondents who are Gen Z and familiar with social media, which was later analysed using SmartPLS. The main findings are advertising value and user attitude significantly affect customers’ purchase intention; advertising value is directly affected by informativeness, entertainment, and credibility; user attitude is directly affected by social interaction, incentives, and irritation. Finally, the research team proposes some solutions for businesses to increase the purchase intention of Gen Z in Vietnam through short-form video advertising on social media.
Comparison of Glucose in Urine with Likening of Pigeons as Pets IIJSRJournal
If someone is liking pigeons as pets, this may be due to their intelligent, effortless, and loving nature. The chief objectives of this study were to relate pigeon lovers as a pet with the level of glucose in their urine. Around 100 students of Bahauddin Zakariya University Multan Pakistan were participants of this study. Pee has glucose that is measured for measuring the glucose in urine. If glucose is not present in the urine it shows the kidney is working well. There is no major effect of glucose in urine with the love of a pigeon as a pet.
Non-unique Fixed Points of Self Mappings in Bi-metric Spaces IIJSRJournal
In this paper, we prove a few non-unique fixed-point results of mapping on a set with bi-metrics using θ – contraction. We also give an example that justifies our results. In the literature, our result generalized many results.
Research on the Impact of Short-Form Video Advertising on Social Media on the...IIJSRJournal
In recent years, short-form video has become a popular form of advertising on social media. The way consumers make decisions to purchase has changed owing to this new marketing method. This study aims to investigate the impact of informativeness, entertainment, credibility, social interaction, incentives and irritation of short-form video advertising on social media on the purchase intention of Gen Z in Vietnam through user attitude and advertising value. A survey was conducted by collecting responses from 1257 respondents who are Gen Z and familiar with social media, which was later analysed using SmartPLS. The findings revealed that advertising value and user attitude significantly affect customers’ purchase intention. In addition, advertising value is directly affected by informativeness, entertainment and credibility. Meanwhile, user attitude is directly affected by social interaction, incentives and irritation. Finally, the research team propose some solutions for businesses to increase the purchase intention of Gen Z in Vietnam through short-form video advertising on social media.
Panchayat Season 3 - Official Trailer.pdfSuleman Rana
The dearest series "Panchayat" is set to make a victorious return with its third season, and the fervor is discernible. The authority trailer, delivered on May 28, guarantees one more enamoring venture through the country heartland of India.
Jitendra Kumar keeps on sparkling as Abhishek Tripathi, the city-reared engineer who ends up functioning as the secretary of the Panchayat office in the curious town of Phulera. His nuanced depiction of a young fellow exploring the difficulties of country life while endeavoring to adjust to his new environmental factors has earned far and wide recognition.
Neena Gupta and Raghubir Yadav return as Manju Devi and Brij Bhushan Dubey, separately. Their dynamic science and immaculate acting rejuvenate the hardships of town administration. Gupta's depiction of the town Pradhan with an ever-evolving outlook, matched with Yadav's carefully prepared exhibition, adds profundity and credibility to the story.
New Difficulties and Experiences
The trailer indicates new difficulties anticipating the characters, as Abhishek keeps on wrestling with his part in the town and his yearnings for a superior future. The series has reliably offset humor with social editorial, and Season 3 looks ready to dig much more profound into the intricacies of rustic organization and self-awareness.
Watchers can hope to see a greater amount of the enchanting and particular residents who have become fan top picks. Their connections and the one of a kind cut of-life situations give a reviving and interesting portrayal of provincial India, featuring the two its appeal and its difficulties.
A Mix of Humor and Heart
One of the signs of "Panchayat" is its capacity to mix humor with sincere narrating. The trailer features minutes that guarantee to convey giggles, as well as scenes that pull at the heartstrings. This equilibrium has been a critical calculate the show's prosperity, resounding with crowds across different socioeconomics.
Creation Greatness
The creation quality remaining parts first rate, with the beautiful setting of Phulera town filling in as a scenery that upgrades the narrating. The meticulousness in portraying provincial life, joined with sharp composition and solid exhibitions, guarantees that "Panchayat" keeps on hanging out in the packed web series scene.
Expectation and Delivery
As the delivery date draws near, expectation for "Panchayat" Season 3 is at a record-breaking high. The authority trailer has previously created critical buzz, with fans enthusiastically anticipating the continuation of Abhishek Tripathi's excursion and the new undertakings that lie ahead in Phulera.
All in all, the authority trailer for "Panchayat" Season 3 recommends that watchers are in for another drawing in and engaging ride. Yet again with its charming characters, convincing story, and ideal mix of humor and show, the new season is set to enamor crowds. Write in your schedules and prepare to get back to the endearing universe of "Panchayat."
From Slave to Scourge: The Existential Choice of Django Unchained. The Philos...Rodney Thomas Jr
#SSAPhilosophy #DjangoUnchained #DjangoFreeman #ExistentialPhilosophy #Freedom #Identity #Justice #Courage #Rebellion #Transformation
Welcome to SSA Philosophy, your ultimate destination for diving deep into the profound philosophies of iconic characters from video games, movies, and TV shows. In this episode, we explore the powerful journey and existential philosophy of Django Freeman from Quentin Tarantino’s masterful film, "Django Unchained," in our video titled, "From Slave to Scourge: The Existential Choice of Django Unchained. The Philosophy of Django Freeman!"
From Slave to Scourge: The Existential Choice of Django Unchained – The Philosophy of Django Freeman!
Join me as we delve into the existential philosophy of Django Freeman, uncovering the profound lessons and timeless wisdom his character offers. Through his story, we find inspiration in the power of choice, the quest for justice, and the courage to defy oppression. Django Freeman’s philosophy is a testament to the human spirit’s unyielding drive for freedom and justice.
Don’t forget to like, comment, and subscribe to SSA Philosophy for more in-depth explorations of the philosophies behind your favorite characters. Hit the notification bell to stay updated on our latest videos. Let’s discover the principles that shape these icons and the profound lessons they offer.
Django Freeman’s story is one of the most compelling narratives of transformation and empowerment in cinema. A former slave turned relentless bounty hunter, Django’s journey is not just a physical liberation but an existential quest for identity, justice, and retribution. This video delves into the core philosophical elements that define Django’s character and the profound choices he makes throughout his journey.
Link to video: https://youtu.be/GszqrXk38qk
From the Editor's Desk: 115th Father's day Celebration - When we see Father's day in Hindu context, Nanda Baba is the most vivid figure which comes to the mind. Nanda Baba who was the foster father of Lord Krishna is known to provide love, care and affection to Lord Krishna and Balarama along with his wife Yashoda; Letter’s to the Editor: Mother's Day - Mother is a precious life for their children. Mother is life breath for her children. Mother's lap is the world happiness whose debt can never be paid.
Are the X-Men Marvel or DC An In-Depth Exploration.pdfXtreame HDTV
The world of comic books is vast and filled with iconic characters, gripping storylines, and legendary rivalries. Among the most famous groups of superheroes are the X-Men. Created in the early 1960s, the X-Men have become a cultural phenomenon, featuring in comics, animated series, and blockbuster movies. A common question among newcomers to the comic book world is: Are the X-Men Marvel or DC? This article delves into the history, creators, and significant moments of the X-Men to provide a comprehensive answer.
Maximizing Your Streaming Experience with XCIPTV- Tips for 2024.pdfXtreame HDTV
In today’s digital age, streaming services have become an integral part of our entertainment lives. Among the myriad of options available, XCIPTV stands out as a premier choice for those seeking seamless, high-quality streaming. This comprehensive guide will delve into the features, benefits, and user experience of XCIPTV, illustrating why it is a top contender in the IPTV industry.
Scandal! Teasers June 2024 on etv Forum.co.zaIsaac More
Monday, 3 June 2024
Episode 47
A friend is compelled to expose a manipulative scheme to prevent another from making a grave mistake. In a frantic bid to save Jojo, Phakamile agrees to a meeting that unbeknownst to her, will seal her fate.
Tuesday, 4 June 2024
Episode 48
A mother, with her son's best interests at heart, finds him unready to heed her advice. Motshabi finds herself in an unmanageable situation, sinking fast like in quicksand.
Wednesday, 5 June 2024
Episode 49
A woman fabricates a diabolical lie to cover up an indiscretion. Overwhelmed by guilt, she makes a spontaneous confession that could be devastating to another heart.
Thursday, 6 June 2024
Episode 50
Linda unwittingly discloses damning information. Nhlamulo and Vuvu try to guide their friend towards the right decision.
Friday, 7 June 2024
Episode 51
Jojo's life continues to spiral out of control. Dintle weaves a web of lies to conceal that she is not as successful as everyone believes.
Monday, 10 June 2024
Episode 52
A heated confrontation between lovers leads to a devastating admission of guilt. Dintle's desperation takes a new turn, leaving her with dwindling options.
Tuesday, 11 June 2024
Episode 53
Unable to resort to violence, Taps issues a verbal threat, leaving Mdala unsettled. A sister must explain her life choices to regain her brother's trust.
Wednesday, 12 June 2024
Episode 54
Winnie makes a very troubling discovery. Taps follows through on his threat, leaving a woman reeling. Layla, oblivious to the truth, offers an incentive.
Thursday, 13 June 2024
Episode 55
A nosy relative arrives just in time to thwart a man's fatal decision. Dintle manipulates Khanyi to tug at Mo's heartstrings and get what she wants.
Friday, 14 June 2024
Episode 56
Tlhogi is shocked by Mdala's reaction following the revelation of their indiscretion. Jojo is in disbelief when the punishment for his crime is revealed.
Monday, 17 June 2024
Episode 57
A woman reprimands another to stay in her lane, leading to a damning revelation. A man decides to leave his broken life behind.
Tuesday, 18 June 2024
Episode 58
Nhlamulo learns that due to his actions, his worst fears have come true. Caiphus' extravagant promises to suppliers get him into trouble with Ndu.
Wednesday, 19 June 2024
Episode 59
A woman manages to kill two birds with one stone. Business doom looms over Chillax. A sobering incident makes a woman realize how far she's fallen.
Thursday, 20 June 2024
Episode 60
Taps' offer to help Nhlamulo comes with hidden motives. Caiphus' new ideas for Chillax have MaHilda excited. A blast from the past recognizes Dintle, not for her newfound fame.
Friday, 21 June 2024
Episode 61
Taps is hungry for revenge and finds a rope to hang Mdala with. Chillax's new job opportunity elicits mixed reactions from the public. Roommates' initial meeting starts off on the wrong foot.
Monday, 24 June 2024
Episode 62
Taps seizes new information and recruits someone on the inside. Mary's new job
Meet Crazyjamjam - A TikTok Sensation | Blog EternalBlog Eternal
Crazyjamjam, the TikTok star everyone's talking about! Uncover her secrets to success, viral trends, and more in this exclusive feature on Blog Eternal.
Source: https://blogeternal.com/celebrity/crazyjamjam-leaks/
Tom Selleck Net Worth: A Comprehensive Analysisgreendigital
Over several decades, Tom Selleck, a name synonymous with charisma. From his iconic role as Thomas Magnum in the television series "Magnum, P.I." to his enduring presence in "Blue Bloods," Selleck has captivated audiences with his versatility and charm. As a result, "Tom Selleck net worth" has become a topic of great interest among fans. and financial enthusiasts alike. This article delves deep into Tom Selleck's wealth, exploring his career, assets, endorsements. and business ventures that contribute to his impressive economic standing.
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Early Life and Career Beginnings
The Foundation of Tom Selleck's Wealth
Born on January 29, 1945, in Detroit, Michigan, Tom Selleck grew up in Sherman Oaks, California. His journey towards building a large net worth began with humble origins. , Selleck pursued a business administration degree at the University of Southern California (USC) on a basketball scholarship. But, his interest shifted towards acting. leading him to study at the Hills Playhouse under Milton Katselas.
Minor roles in television and films marked Selleck's early career. He appeared in commercials and took on small parts in T.V. series such as "The Dating Game" and "Lancer." These initial steps, although modest. laid the groundwork for his future success and the growth of Tom Selleck net worth. Breakthrough with "Magnum, P.I."
The Role that Defined Tom Selleck's Career
Tom Selleck's breakthrough came with the role of Thomas Magnum in the CBS television series "Magnum, P.I." (1980-1988). This role made him a household name and boosted his net worth. The series' popularity resulted in Selleck earning large salaries. leading to financial stability and increased recognition in Hollywood.
"Magnum P.I." garnered high ratings and critical acclaim during its run. Selleck's portrayal of the charming and resourceful private investigator resonated with audiences. making him one of the most beloved television actors of the 1980s. The success of "Magnum P.I." played a pivotal role in shaping Tom Selleck net worth, establishing him as a major star.
Film Career and Diversification
Expanding Tom Selleck's Financial Portfolio
While "Magnum, P.I." was a cornerstone of Selleck's career, he did not limit himself to television. He ventured into films, further enhancing Tom Selleck net worth. His filmography includes notable movies such as "Three Men and a Baby" (1987). which became the highest-grossing film of the year, and its sequel, "Three Men and a Little Lady" (1990). These box office successes contributed to his wealth.
Selleck's versatility allowed him to transition between genres. from comedies like "Mr. Baseball" (1992) to westerns such as "Quigley Down Under" (1990). This diversification showcased his acting range. and provided many income streams, reinforcing Tom Selleck net worth.
Television Resurgence with "Blue Bloods"
Sustaining Wealth through Consistent Success
In 2010, Tom Selleck began starring as Frank Reagan i
In the vast landscape of cinema, stories have been told, retold, and reimagined in countless ways. At the heart of this narrative evolution lies the concept of a "remake". A successful remake allows us to revisit cherished tales through a fresh lens, often reflecting a different era's perspective or harnessing the power of advanced technology. Yet, the question remains, what makes a remake successful? Today, we will delve deeper into this subject, identifying the key ingredients that contribute to the success of a remake.
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Monday, June 3, 2024 - Episode 241: Sergeant Rathebe nabs a top scammer in Turfloop. Meikie is furious at her uncle's reaction to the truth about Ntswaki.
Tuesday, June 4, 2024 - Episode 242: Babeile uncovers the truth behind Rathebe’s latest actions. Leeto's announcement shocks his employees, and Ntswaki’s ordeal haunts her family.
Wednesday, June 5, 2024 - Episode 243: Rathebe blocks Babeile from investigating further. Melita warns Eunice to stay clear of Mr. Kgomo.
Thursday, June 6, 2024 - Episode 244: Tbose surrenders to the police while an intruder meddles in his affairs. Rathebe's secret mission faces a setback.
Friday, June 7, 2024 - Episode 245: Rathebe’s antics reach Kganyago. Tbose dodges a bullet, but a nightmare looms. Mr. Kgomo accuses Melita of witchcraft.
Monday, June 10, 2024 - Episode 246: Ntswaki struggles on her first day back at school. Babeile is stunned by Rathebe’s romance with Bullet Mabuza.
Tuesday, June 11, 2024 - Episode 247: An unexpected turn halts Rathebe’s investigation. The press discovers Mr. Kgomo’s affair with a young employee.
Wednesday, June 12, 2024 - Episode 248: Rathebe chases a criminal, resorting to gunfire. Turf High is rife with tension and transfer threats.
Thursday, June 13, 2024 - Episode 249: Rathebe traps Kganyago. John warns Toby to stop harassing Ntswaki.
Friday, June 14, 2024 - Episode 250: Babeile is cleared to investigate Rathebe. Melita gains Mr. Kgomo’s trust, and Jacobeth devises a financial solution.
Monday, June 17, 2024 - Episode 251: Rathebe feels the pressure as Babeile closes in. Mr. Kgomo and Eunice clash. Jacobeth risks her safety in pursuit of Kganyago.
Tuesday, June 18, 2024 - Episode 252: Bullet Mabuza retaliates against Jacobeth. Pitsi inadvertently reveals his parents’ plans. Nkosi is shocked by Khwezi’s decision on LJ’s future.
Wednesday, June 19, 2024 - Episode 253: Jacobeth is ensnared in deceit. Evelyn is stressed over Toby’s case, and Letetswe reveals shocking academic results.
Thursday, June 20, 2024 - Episode 254: Elizabeth learns Jacobeth is in Mpumalanga. Kganyago's past is exposed, and Lehasa discovers his son is in KZN.
Friday, June 21, 2024 - Episode 255: Elizabeth confirms Jacobeth’s dubious activities in Mpumalanga. Rathebe lies about her relationship with Bullet, and Jacobeth faces theft accusations.
Monday, June 24, 2024 - Episode 256: Rathebe spies on Kganyago. Lehasa plans to retrieve his son from KZN, fearing what awaits.
Tuesday, June 25, 2024 - Episode 257: MaNtuli fears for Kwaito’s safety in Mpumalanga. Mr. Kgomo and Melita reconcile.
Wednesday, June 26, 2024 - Episode 258: Kganyago makes a bold escape. Elizabeth receives a shocking message from Kwaito. Mrs. Khoza defends her husband against scam accusations.
Thursday, June 27, 2024 - Episode 259: Babeile's skillful arrest changes the game. Tbose and Kwaito face a hostage crisis.
Friday, June 28, 2024 - Episode 260: Two women face the reality of being scammed. Turf is rocked by breaking
As a film director, I have always been awestruck by the magic of animation. Animation, a medium once considered solely for the amusement of children, has undergone a significant transformation over the years. Its evolution from a rudimentary form of entertainment to a sophisticated form of storytelling has stirred my creativity and expanded my vision, offering limitless possibilities in the realm of cinematic storytelling.
2. Asian Journal of Applied Science and Technology (AJAST)
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17
concentration was tried in formulation to understand the survival life of Azotobacter and interaction between
Azotobacter and formulation materials.
Material and Methods
Jensen’s Basal Medium: The N2 free Jensen’s medium (Jensen 1954) containing 20 gm sucrose, 1.0 gm K2HPO4,
0.5 gm MgSO4.7H2O, 0.5 gm NaCl, 2 gm CaCO3, 0.005 gm Na2MoO4, and 0.1 gm FeSO4 dissolved in 1 lit
Distilled water and pH was adjusted to 7.1
Liquid State Formulation with polymer cell protectants: For the standardization of proper quantity of
amendments and to find out the survival time of Azotobacter chroococcum the polymer cell protectants were added
with Jensen’s basal medium. These various formulation were as follows
(1) Basal medium (Control).
(2) Basal medium + Glycerol with 5, 10, 15, 20, 25 mM.
The slant culture of Azotobacter along with above formulation was incubated for 4 days on shaker at 121 rpm. The
optical densities 0.25 were adjusted at 0.25 with the help of spectrophotometer. This 10 ml culture of Azotobacter
chroococcum was inoculated in each flask of above 5 types of liquid formulation under aseptic condition. The
broths were kept for continuous shaking on rotary shaker for 72 hours at 121 rpm, and then the cultures were
transferred in sterilized glass bottle, plugged with cotton and stored at room temperature. These broth cultures were
tested for total viable count at 30 days interval up to 12 months.
(3) Basal Medium + Glycerol 25 mM only- This formulation was prepared to compare the growth and survival of
Azotobacter chroococcum A1 Azotobacter chroococcum A2, Azotobacter chroococcum NCIM 5576 isolates.
These isolates were inoculated (10 ml of culture) separately in each flask under aseptic condition. The broths were
incubated on rotary shaker for 72 hours at 121 rpm, and then cultures were transferred in sterilized glass bottle,
plugged and stored at 300
C temperature. These broth cultures were tested for total viable count at 30 days interval
up to 12 months.
Table1. Effect of Glycerol (CFU/ml at 109
) in Jensen's broth on survival of Azotobacter chroococcum A1 colonies
Days
Broth Alone
(control)
Glycerol
5 mM
Glycerol
10 mM
Glycerol
15 mM
Glycerol
20 mM
Glycerol
25 mM
CD at 0.05%
0 22.66a±0.51
23.00a±0.58
(1.50)
30.00b±0.58
(32.39)
31.00c ±0.58
(36.80)
34.66d±0.51
(50.04)
38.66e±0.51
(70.61)
0.9 4.20E-10
30 20.33a±0.58
23.00a±0.58
(13.13)
28.00c ±0.58
(37.73)
29.00d±0.58
(42.65)
34.33e±0.69
(68.86)
38.33f±0.69
(88.54)
0.47 8.66E-10
60 16.66a±0.38
21.00b±0.58
(26.05)
26.00c±0.58
(56.06)
28.00d±0.58
(68.07)
32.00c±0.58
(92.08)
37.00f± 0.58
(122.09)
0.42 7.84E-11
90 14.00a±0.58
20.00b±0.58
(42.86)
24.00c±0.58
(71.43)
25.00d±0.58
(78.57)
30.00e±0.58
(114.29)
35.00f±0.32
(150.00)
0.44 1.35E-10
120 10.00a±0.58
17.00b±0.58
(70.00)
22.00c ±0.58
(120.00)
22.00c±0.58
(120.00)
28.00d±0.58
(180.00)
33.66e±0.32
(236.60)
0.42 1.71E-11
3. Asian Journal of Applied Science and Technology (AJAST)
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150 8.00a±0.58
16.00b±0.58
(100.00)
20.00c ±0.58
(150.00)
21.00d±0.58
(162.50)
25.00e±0.58
(212.50)
33.33f±0.51
(316.63)
0.43 2.00E-11
180 5.33a±0.31
14.00b± 0.58
(162.66)
20.00c ±0.58
(275.23)
21.66d±0.50
(306.38)
25.00e±0.58
(369.04)
32.00f±0.58
(500.38)
0.4 3.52E-12
Data presented are means of ten readings; values within the same row with different letters are significantly
different at 0.05% P-level by Single factor ANOVA test followed by CD & Tukey's test. [Figures in parentheses
indicate % increase (+) and % decrease (-) over control; ± standard error of mean; CD:critical difference; P-value/
alpha value at 0.05%; CFU/ml At 109
: Colony forming unit per gram per ml of Glycerol plus Jensen's broth).
Table 2. Effect of Glycerol (CFU/ml at 109
) in Jensen's broth on survival of Azotobacter chroococcum A1 colonies
Data presented are means of ten readings; values within the same row with different letters are significantly
different at 0.05% P-level by Single factor ANOVA test followed by CD & Tukey's test. [Figures in parentheses
indicate % increase (+) and % decrease (-) over control; ± standard error of mean; CD: critical difference; P-value/
alpha value at 0.05%; where CFU/ml AT 109
:Colony forming unit per gram per ml of Glycerol plus Jensen's broth).
Table 3. Effect of Glycerol in Jensen's broth on survival of A1,A2 and NCIM 5576 strains of Azotobacter
chroococcum during Storage, at 109
CFU/ml
Name of the bacterium Survival of colonies
(average number )
Days
Azotobacter chroococcumA1 40.69a±2.90 0 to 360
Azotobacter chroococcum A2 46.85b±2.85 1 to 360
Azotobacterchroococcum NCIM 33.00c±1.18 2 to 360
CD at 0.05% 3.1
P-value at 0.05% 0.001
Days
Broth Alone
(control)
Glycerol
5 mM
Glycerol
10 mM
Glycerol
15 mM
Glycerol
20 mM
Glycerol
25 mM
CD at 0.05%
210 4.00a±0.58
12.00b±0.58
(200.00)
17.00c±0.58
(325.00)
21.33d±0.62
(433.25)
23.66e ±0.61
(491.50)
30.00f±0.58
(650.00)
0.45 1.30E-11
240 3.00a±0.58
10.66b± 0.20
(255.33)
16.00c±0.58
(433.33)
20.00d±0.58
(566.67)
23.33e±0.56
(677.67)
28.00f±0.58
(833.33)
0.84 4.45E-12
270 1.33a±0.07
10.33b± 0.46
(676.69)
15.00c±0.58
(1027.82)
18.00d±0.58
(1253.38)
22.00e±0.58
(1554.14)
27.00f±0.58
(1930.08)
0.39 2.61E-12
300 0.00a±0.00 9.00b±0.58 13.00c±0.58 17.00d±0.58 20.00e±0.58 25.00f±0.58 0.4 5.54E-12
330 0.00a±0.00 7.00b±0.58 10.00c±0.58 15.00d±0.58 18.00e±0.58 23.00f±0.58 0.4 1.24E-11
360 0.00a±0.00 5.00b±0.58 9.00c±0.58 14.00d±0.58 17.00e±0.58 22.00f±0.58 0.4 1.53E-11
4. Asian Journal of Applied Science and Technology (AJAST)
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19
Fig.1. Effect of different concentrations (5 mM to 25 mM) of Glycerol in Jensen's broth on survival of Azotobacter
chroococcum colonies
Fig.2. Effect of Glycerol in Jensen's broth on survival of A1, A2 and NCIM 5576 strains of Azotobacter
chroococcum during Storage, at 109
CFU/ml
0
5
10
15
20
25
30
35
40
45
0 30 60 90 120 150 180 210 240 270 300 330 360
Number
of
survived
colonies
Days
Effect of different concentrations of Glycerol on survival of
Azotobacter colonies
Broth (control)
5mM
10mM
15mM
20mM
25mM
27
32
26
0
10
20
30
40
50
60
70
0 30 60 90 120 150 180 210 240 270 300 330 360
Number
of
colonies
Days
Effect of Glycerol on survival of bacteria during
storge at 109CFU/ML
A.chroococcumA1
A. chroococcum A2
A. chroococcum NCIM
5. Asian Journal of Applied Science and Technology (AJAST)
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20
.
Photo plate 1. Effect of Glycerol in Jensen's broth on survival of A1, A2 and NCIM 5576 strains of
Azotobacter chroococcum during Storage, at 109
CFU/ml
Result and Discussion
The experimental work was carried out for the survival of Azotobacter in liquid and solid formulations. Many
researchers have shown that liquid rhizobial formulations are more beneficial. Hynes et al (1995, 2001) revealed
that the results of liquid rhizobial formulations for the growth and yield of crops are more suitable than that of
peat-based products. Maniknandan et al (2010) worked on standardization of liquid formulation of Psudomonas
fluorescens by using , trehalose, polyvinylpyrrolidone and glycerol for development of liquid formulation. Among
these, glycerol amendment maintained the greater population level of P. fluorescens Pf1 up to 6 months of
storage.Cortes-Patino Sandra and Bonilla Ruth Rebeca (2015) worked on Polymers selection for a liquid inoculant
of Azospirillum brasilense based on the Arrhenius thermodynamic modelthey used a method of accelerated
degradation to select a polymer and a concentration to maintain cell stability of a liquid inoculant based on the
strain C16 Azospirillum brasilense. A screening at 45°C was made to compare the protectant effect of five
polymers on the viability of the strain (p/v): carrageenan (1.5%), sodium alginate (1%), trehalose (10 mM),
polyvinylpyrrolidone (2%), glycerol (10 mM) and phosphate saline buffer as control. conclude that these polymers
can be used for a formulation of a liquid inoculant based on the strain C16 Azospirillum brasilense.
It was evaluated by using polymers like polyvinyl pyrollidone (PVP), Glycerol, trehalose, separately and PVP,
Glycerol, trehalose together. Simultaneously the survival was also evaluated by using different carbon sources
along with Jensen’s basal medium. The results are depicted in following tables. Data was tabulated and analyzed
6. Asian Journal of Applied Science and Technology (AJAST)
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21
statistically, presented in graphical form also. Azotobacter cells in Jenson’s broth alone were used as control for
liquid formulation and lignite was used for solid formulation. The different additives were tried in formulation to
understand the survival life of Azotobacter and interaction between Azotobacter and formulation materials.
(1) Effect of glycerol on survival of Azotobacterchroococcum A1 colonies grown in Jensen’s medium (Table No
15, 16, Figure No 19): Glycerol is a sweet-tasting, non-toxic substance that contain a propane molecule attached to
three –Oh groups. Different concentrations viz. 10 mM to 25 mM were prepared and added to the culture of
Azotobacter chroococcum grown in Jensen’s medium. Jensen’s broth/medium without addition of glycerol was
treated as control. Effect of all concentrations on survival of Azotobacter colonies was significant at 0.05%
probability level. On zero days highest number of colonies was recorded in medium containing 25 mM Trehalose
followed by 20 mM, 15 mM, 10 mM and 5 mM in the medium containing glycerol. Survival of the Azotobacter was
maintained up to 360 days. It was noted that survival of Azotobacter was concentration correlated. Lowest number
of colonies in 5mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th
day) and
highest number of colonies in25mM in the medium containing glycerol (38.66 colonies on zero day and 22 colonies
on 360th
day) (109
CFU/ml). Santhosh (2015) recorded similar results. Colonies (22.66 on zero day, 1.33 colonies
on 270th
day) in pure broth could be maintained. Effect of glycerol on survival of Azotobacterchroococcum
colonies grown in Jensen’s medium has shown better result of colony count (22 colonies on 360th
day) over control
at room temperature.
Stock culture of Azotobacter (SR-4) was maintained by Misbahud Dineet al (2019) on nutrient agar slants and
glycerol cultures in nutrient broth and was stored at -800
C. Rojas-Tapias et al (2013) stated that use of preservation
techniques is usually accompanied with use of protective agents, which can increase effectiveness of the technique
preventing cell damage. For this reason, selection of the protective agent like glycerol depends on the preservation
method and type of bacteria.
As per the previous records, our efforts for survival of Azotobacter are successful and economical by using
Glycerol as preservative. Lorda and Balatti (1996) recorded that glycerol (10 mM) showed better population
survival of Azotobacter next to trehalose may which may be due to high water holding capacity and may protect
cell from the effect if desiccation by reducing rate of drying.
(2) The result indicate that, maximum population of Azotobacter was recorded in, glycerol 25 mM 22x109
cfu/ml,
followed by pvp 3%16x109
cfu/ml, trehalose (25 Mm)14x109
cfu/ml, combine use of trehalose, and glycerol 6x107
cfu/ml where as minimum population was recorded in control( Jensen broth only) 4x104
cfu/ml during storage of 12
months.
Santhosh (2015) formulated Rhizobium, Azotobacter, Azospirillium, and Bacillus megaterium and stored these
formulations in BOD incubator for 180 days and total viable count was analyzed at 30 days interval he recorded
0.5% polyvinylpyrrolidone in addition with 0.5% glycerol recorded maximum cell count in all cultures followed by
polyethelene glycerol, gum Arabic and sodium alginate. And also reported that, the survival of bacteria in liquid
formulation was increased due to the improvement in biological integrity, this was because polyvinyl pyrrolidone
which helps to reduce extra protein coagulation of bacterial cell and macromolecular structure maintenance.
7. Asian Journal of Applied Science and Technology (AJAST)
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22
Polyvinyl pyrrolidone helps to maintain moisture level in medium (Singleton et al 2002; Deaker et al 2004). Also
Polyvinyl pyrrolidone binds with bacterial toxins released in medium, during stationary phase of bacteria
(Errington et al (2002), Deaker et al (2004), C.F. Dayamani and Brahmaprakash (2014).
Conclusion
It was concluded that survival of Azotobacter along with deferent concentrations of glycerol was concentration
correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5
colonies on 360th
day) and highest number of colonies in 25 mM in the medium containing glycerol (38.66 colonies
on zero day and 22 colonies on 360th
day) at (109
CFU/ml) was recorded.
Declarations
Source of Funding
This research did not receive any grant from funding agencies in the public, commercial, or not-for-profit sectors.
Competing Interests Statement
The authors declare no competing financial, professional and personal interests.
Consent for publication
Authors declare that they consented for the publication of this research work.
References
Baral, B.R., & Adhikari, P., (2013). Effect of azotobacter on growth and yield of maize. SAARC J. Agri., 11(2):
141-147.
Cortes-Patino Sandra & Bonilla Ruth Rebeca, (2015). Polymers selection for a liquid inoculant of azospirillum
brasilense based on the arrhenius thermodynamic model. African Journal of Biotechnology, 14(33): 2547-2553.
Deaker, R., Roughley, R.J., & Kennedy, I.R., (2004). Legume seed inoculation technology–a review. Soil Biol
Biochem., 36: 1275-88.
Dayamani, K.J., & Brahmaprakash, G.P., (2014). Influence of forms and concentrations of the osmolytes in liquid
inoculants formulations of plant growth promoting bacteria. International Journal of Scientific and Research
Publications, 4(7): 2250-3153.
Errington, J.R., Pablo, G.D., & Brian, A.P., (2002). The stability of proteins in a polyvinylpyrrolidone matrix.
Department of Chemical Engineering, Princeton University, USA.
Gandhi, A., & Saravanakumar K., (2009). Studies on shelf-life of Azospirillum lipoferum, Bacillus megaterium and
Pseudomonas fluorescens in Vemicompost Carrier. J. Phytol., 1: 75-85.
Ghosh, N., (2004). Promoting biofertilisers in Indian agriculture., Economic and Political Weekly, 39(52):
5617-5625.
8. Asian Journal of Applied Science and Technology (AJAST)
Volume 5, Issue 4, Pages 16-23, Oct-Dec 2021
ISSN: 2456-883X www.ajast.net
23
Hynes, R.K., Jans, D.C., Bremer, E., Lupwayi, N.Z., Rice, W.A., Clayton, G.W., & Collins, M.M., (2001).
Rhizobium population dynamics in pea rhizosphere of rhizobial inoculant strain applied in different formulations.
Can. J. Microbiol., 47: 595-600.
Hynes, R.K., Kraig, K.A., Covert, D., Smith, R.S., & Rennie, R.J., (1995). Liquid rhizobial inoculants for lentil and
field pea. J. Prod. Agric., 8: 547-552.
Jensen, H., (1954). The Azotobacteriaceae Bacteriol Rev., Dec 14(4): 195-214.
Lorda, G., & Balatti, A., (1996). Designing mediaI and II: Legume inoculants. Selection and characterization of
strains, production, use and management (eds) Balatti and Freise, Editorial Kingraf, Buenos Aires.
Maniknandan, R., Sasravanakumar, D., Rajendaran, L., Raguchander, T., Samiyappan, R., (2010). Standardization
of liquid formulation of Pseudomonas fluorescens PF1 for its Efficacy against Fusarium wilt of tomato. J. Biol.
Control, 54(2): 83-89.
Misbahud Din, Rubina Nelofer, Muhammad Salman, Abdullah, Faisal Hayat Khan, Asad Khan, Munib Ahmad,
Fazal Jalil, Jalal Ud Din, Mudassir Khan, (2019). Production of nitrogen fixing Azotobacter (SR-4) and phosphorus
solubilizing Aspergillus niger and their evaluation on Lagenaria siceraria and Abelmoschus esculentus.
Biotechnology Reports, 20: 1-5. http://creativecommons.org/licenses/by/4.0.
Mohd Mazid, & Taqi Ahmed Khan, (2014). Future of bio-fertilizers in indian agriculture: an overview.
International Journal of Agricultural and Food Research, 3(3): 10-23.
Mungier, J., & Jung, G., (1985). Survival of bacteria and fungi in relation to water activity and the solvent
properties of water in biopolymer. Appl. Environ. Microbiol., 50: 108-14.
Santhosh, G.P., (2015). Formulation and shelf life of liquid bio fertilizer inoculants using cell protectants.
Intentional Journal of Researches in Biosciences Agriculture and Technology, 11(7): 243-247.
Singleton, P., Keyser, H., & Sande, E., (2002). ACIAR proceding loge herridge D. (ed) Australian centre for
international Agricultural research Canberra, Australia Development and evolution of liquid inoculants, In
Inoculants and nitrogen fixation of legumes in veitham, 52-60.
Somasengaran, P., & Hoben, J.H., (1994). Handbook of rhizobia method in legume–rhizobium technology,
Springer-Verlag, New York Inc., New York.