Chromosome painting is a technique that uses fluorescent in-situ hybridization (FISH) to identify human chromosomes. It involves hybridizing a labeled DNA probe to a target DNA sequence within a cell nucleus. Different types of probes like centromeric, telomeric, whole chromosome, and locus-specific can be used with multiple colors to detect single or multiple genetic events within a cell without destroying its morphology. The procedure involves preparing samples on slides, using probes labeled with fluorescent molecules, and visualizing the signals under a fluorescence microscope equipped with appropriate filters. Chromosome painting has applications in locating genes, detecting translocations and abnormalities, and prenatal genetic disorder diagnosis.