This document discusses human chromosomes and karyotypes. It begins by explaining that each species has a specific number of chromosomes, and that humans do not have the greatest number. It then provides details on human chromosomes, including that humans have 46 total chromosomes composed of 23 pairs, with 22 pairs being autosomes and 1 pair being sex chromosomes that determine gender as either XX or XY. The document explains how to read a karyotype, including identifying the numbered chromosome pairs and whether it represents a male or female. It concludes with assigning a karyotype lab for students to complete.
The topic explains briefly on central dogma of molecular biology, DNA packaging in chromosome, to understanding the nature of genetics Code and to compare the mitochondria & chloroplast DNA with nuclear DNA
Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
“Cytogenetics traditionally refers to the study of chromosomes by microscopy following the application of banding techniques, permitting identification of abnormalities of chromosome number, loss or gain of chromosomal material or positional changes”. Standardized nomenclature is critical for the accurate and consistent description of genomic changes as identified by karyotyping, fluorescence in situ hybridization and microarray. The International System for Human Cytogenomic Nomenclature (ISCN) is the central reference for the description of karyotyping, FISH, and microarray results, and provides rules for describing cytogenetic and molecular cytogenetic findings in laboratory reports. These laboratory reports are documents to the referring clinician, and should be clear, accurate and contain all information relevant for good interpretation of the cytogenetic findings.
The topic explains briefly on central dogma of molecular biology, DNA packaging in chromosome, to understanding the nature of genetics Code and to compare the mitochondria & chloroplast DNA with nuclear DNA
Karyotyping is the process by which photographs of chromosomes are taken in order to determine the chromosome complement of an individual, including the number of chromosomes and any abnormalities.
The term is also used for the complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or measures the number.
“Cytogenetics traditionally refers to the study of chromosomes by microscopy following the application of banding techniques, permitting identification of abnormalities of chromosome number, loss or gain of chromosomal material or positional changes”. Standardized nomenclature is critical for the accurate and consistent description of genomic changes as identified by karyotyping, fluorescence in situ hybridization and microarray. The International System for Human Cytogenomic Nomenclature (ISCN) is the central reference for the description of karyotyping, FISH, and microarray results, and provides rules for describing cytogenetic and molecular cytogenetic findings in laboratory reports. These laboratory reports are documents to the referring clinician, and should be clear, accurate and contain all information relevant for good interpretation of the cytogenetic findings.
B4FA 2012 Nigeria: Principles of Genetics - Charles Amadib4fa
Presentation by Dr Charles Amadi, National Root Crops Research Centre, Umudike, Nigeria
Delivered at the B4FA Media Dialogue Workshop, Ibadan, Nigeria - September 2012
www.b4fa.org
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
1. Think. Pair. Share:
• Do humans have the greatest number of
chromosomes per cell because we are “the
most intelligent animals on Earth?”
– Why or why not? Write your ideas down in your
notebook
– Share with a partner
– Discuss as a class
5. Be aware that
• So no… we don’t have the greatest amount of
chromosomes and the number of chromosomes
does not relate to intelligence
• Some species of organisms have the same
number of chromosomes: (48)
– Potatoes
– Plums
– Orangutan
– Gorilla
• FOR THE MOST PART each species has a
different number
6. Humans & Chromosomes
• Total of 46 individual chromosomes
– kid gets an equal amount of chromosomes from
mom & dad
• 23 from mom’s egg = 1 set = haploid (1n)
• 23 from dad’s sperm = 1 set = haploid (1n)
8. • Chromosomes come in pairs (2)
• If we know we have 46 individual
chromosomes… how many pairs do we have?
– 46 ÷ 2 = 23 pairs
• Humans have 23 pairs of chromosomes
46
individual 23 pairs
10. • Photograph shows the complete set of
chromosomes for an organism
• Photograph organizes chromosomes by pairs
– Pairs are matched together as homologous
chromosomes
– You can look at a karyotype and identify:
1. Species
2. Sex
Karyotype
12. • Sex chromosomes
– Carries genetic information: male or female
• Autosomes
– Carries genetic information: what the species
will look like
– Information for everything else the cell requires
to live
2 Types of Chromosomes
13. • 2 chromosomes (1 pair) are called sex
chromosomes
– Always the last pair, #23, in the photograph
• They determine your sex
– Female
– Male
• If you are a female you get XX (large in size)
• If you are a male you get XY (X is large but Y is
only ½ the size)
Sex Chromosomes
15. • Other 44 chromosomes (22 pairs) are called
autosomes
– Perfectly matched up
– The first 22 pairs in the photograph
• They tell us what the species will look like, and all
other information required for the cell to live
• Get 1 copy from mom, 1 copy from dad to get a
pair
– 2 copies of each autosome are called homologous
chromosomes; same size, shape and genes
Autosomes
17. Human Karyotyping
• How do you write what you see in a
karyotype of a healthy, normal male
or female? “#, sex chromosomes”
Male = 46, XY
Female = 46, XX
18. • Chromosomes are in ordered pairs
numbered 1-23
– Pairs 1 through 22 are the autosomes
– The last pair, the 23rd pair, are the sex chromosomes
(XX or XY)
• Pairs are matched up as close as possible, based
on their “bands” or “stripes” which are genes
– These are the HOMOLOGOUS chromosomes
How to read a Karyotype
19.
20.
21. • Egg carry 23 chromosomes
– 1 is an X sex chromosome
• Sperm carry 23 chromosomes
– 50:50 chance that the sex chromosome is
X or Y
Who determines the sex of the child?
Sex Determination
Males, fathers
22. KARYOTYPE LAB
• Pass out the packets.
• Go over instructions together.
• PART C in class today
– Materials on front table
23. Homework
• Section 1 review:
– Questions 1-8
– Complete sentences for all except:
• 1 and 5
Editor's Notes
Why or why not? Write your ideas down in your notebookShare with a partnerDiscuss as a class
- Have a student to come up and label this chromosome
Different kinds of organisms or living things have a different number of chromosomes specific to them, we are going to worry about us, humansPlants like a fern, Jurassic Park movies has the highest known chromosome number of any life form 1260 Dog has 78; ant has 2 chromosomesClosest relative to us, the primates (chimpanzee) has 48
Homo sapien = “wise man” Latin, using binomial nomenclature (bicycle = 2 wheels, 2 names)- Haploid = contain only 1 set of chromosomes
The chromosomes you get from each parent are randomly given to you by chance, that’s what those Punnett Squares told us…Diploid = 2 sets of chromosomes; have 2 autosomes for each homologous pair
Always come in pairs unless we have a mutation…. Then we can get 1 or 3… get diseases, deformities, or conditions
- Students might be familiar with it already if they have seen shows like this crime shows, law and order, Greys, someone has a job in a lab to make these karyotypes… use to diagnose disease, look at traits that can be inherited
What species scientific name of dog, cat, worm, flower, etc…. We are Homo sapiens - because of the banding on the chromosomes and the number of the chromosomesSex is it male or female
All of the chromosomes a normal, healthy human should have
What we look like what our species chromosomes look like (4 legs for a dog, humans get 2 arms and 2 legs)
This human karyotype shows the last pair is male with an x and y
So now we can differentiate species 4 legs, or 2 arms and 2 legs, going to have wings/feathers/fins/etc…- Homologous chromosomes= contains a gene for eye color, so will the other chromosome in the homologous pair
#, letters
Centromere = center, middle Chromatid + chromatid = chromosome held by centromere
Red = ? Sex chromosomes what sex is it? Male… how do you know?Blue = ? Autosomes #5, 8, 16, 21
What is this a karyotype of? How do you know?- female, has XX so get two Xs and they are of the same size
Females only have the X so they give an x no matter whatMales could give the X or Y… if they give the x you are a girl, if they gave the y then you are a boy- Show this on the table
Lab will be continued on ThursdayCollect student work in plastic bags w name on it