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Chapter 15 Microbial Mechanisms of Pathogenicity
Microbial Mechanisms of Pathogenicity ,[object Object],[object Object]
[object Object],[object Object],[object Object],Portals of Entry
[object Object],[object Object],[object Object],[object Object],Numbers of Invading Microbes
Bacillus anthracis 250,000-1,000,000 endospores Ingestion 10,000-20,000 endospores Inhalation 10-50 endospores Skin ID 50 Portal of entry
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Adherence
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Enzymes
Penetration into the Host Cell Figure 15.2 Invasins –   rearrange actin fibers associated with host cell membrane Salmonella, E. coli Causes membrane ‘ruffling” –  result of cytoskeletal disruption Microbe sinks into ruffle and is  Ingested by cell. Actin can be used as propulsion once inside host cell (s higella spp)
Toxins ,[object Object],[object Object],[object Object],[object Object],[object Object]
Exotoxin – many are enzyme-like Small LD 50 Yes  (good antigen) Neutralized by antitoxin No (mostly…except type 1) Fever? Protein Chemistry By-products of growing cell Metabolic product Mostly Gram + Source
[object Object],Exotoxins Figure 15.5
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Exotoxins
[object Object],[object Object],[object Object],Exotoxins
Exotoxins Superantigen. Enterotoxin. •  Staphylococcus aureus + A-B toxin. Enterotoxin •  Vibrio cholerae A-B toxin. Neurotoxin •  C. tetani + A-B toxin. Neurotoxin •  Clostridium botulinum + Membrane-disrupting. Erythrogenic. •  Streptococcus pyogenes + A-B toxin. Inhibits protein synthesis. •  Corynebacterium diphtheriae Lysogenic conversion Exotoxin
Exotoxins Figure 15.4a
Endotoxin Figure 15.4b
Endotoxins Figure 15.6 (A cytokine) TNF – tumor necrosis factor – another cytokine secreted by phagocytes– damages capillaries
Endotoxins Relatively large LD 50 No (poor antigen) Neutralized by antitoxin Yes Fever? Lipid-polysaccharide Chemistry Present in LPS of outer membrane Metabolic product Gram– Source
Test for endotoxin presence Endotoxins are heat resistant, may persist on surfaces. LAL- Limulus Amoebocyte Lysate – presence of minute amounts  of endotoxin lyses amoebocytes which release lysate Lysate then causes coagulation of the media
Cytopathic Effects of Viruses Table 15.4
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Pathogenic Properties of Fungi
[object Object],[object Object],[object Object],[object Object],[object Object],Pathogenic Properties of Fungi
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Pathogenic Properties of Protozoa
[object Object],[object Object],[object Object],[object Object],[object Object],Pathogenic Properties of Helminths
[object Object],[object Object],[object Object],Pathogenic Properties of Algae
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Portals of Exit
Mechanisms of Pathogenicity Figure 15.9

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Ch15 Tolli

  • 1. Chapter 15 Microbial Mechanisms of Pathogenicity
  • 2.
  • 3.
  • 4.
  • 5. Bacillus anthracis 250,000-1,000,000 endospores Ingestion 10,000-20,000 endospores Inhalation 10-50 endospores Skin ID 50 Portal of entry
  • 6.
  • 7.
  • 8. Penetration into the Host Cell Figure 15.2 Invasins – rearrange actin fibers associated with host cell membrane Salmonella, E. coli Causes membrane ‘ruffling” – result of cytoskeletal disruption Microbe sinks into ruffle and is Ingested by cell. Actin can be used as propulsion once inside host cell (s higella spp)
  • 9.
  • 10. Exotoxin – many are enzyme-like Small LD 50 Yes (good antigen) Neutralized by antitoxin No (mostly…except type 1) Fever? Protein Chemistry By-products of growing cell Metabolic product Mostly Gram + Source
  • 11.
  • 12.
  • 13.
  • 14. Exotoxins Superantigen. Enterotoxin. • Staphylococcus aureus + A-B toxin. Enterotoxin • Vibrio cholerae A-B toxin. Neurotoxin • C. tetani + A-B toxin. Neurotoxin • Clostridium botulinum + Membrane-disrupting. Erythrogenic. • Streptococcus pyogenes + A-B toxin. Inhibits protein synthesis. • Corynebacterium diphtheriae Lysogenic conversion Exotoxin
  • 17. Endotoxins Figure 15.6 (A cytokine) TNF – tumor necrosis factor – another cytokine secreted by phagocytes– damages capillaries
  • 18. Endotoxins Relatively large LD 50 No (poor antigen) Neutralized by antitoxin Yes Fever? Lipid-polysaccharide Chemistry Present in LPS of outer membrane Metabolic product Gram– Source
  • 19. Test for endotoxin presence Endotoxins are heat resistant, may persist on surfaces. LAL- Limulus Amoebocyte Lysate – presence of minute amounts of endotoxin lyses amoebocytes which release lysate Lysate then causes coagulation of the media
  • 20. Cytopathic Effects of Viruses Table 15.4
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  • 26.