Biosensors integrate a biological recognition element with a physiochemical transducer to produce a measurable signal proportional to the analyte concentration. There are several key components of a biosensor including the bioreceptor, transducer, and detector. Common types of biosensors include optical, resonant, physical, ion-sensitive, and electrochemical biosensors. Biosensors offer advantages like specificity, rapid response, and continuous monitoring capability. They have wide applications in fields like medical diagnostics, environmental monitoring, food analysis, and industrial process control.
A biosensor is an analytical device containing an immobilized biological material (enzyme, antibody, nucleic acid, hormone, organelle or whole cell) which can specifically interact with an analyte and produce physical, chemical or electrical signals that can be measured. An analyte is a compound (e.g. glucose, urea, drug, pesticide) whose concentration has to be measured.
Biosensor .now what is biosensor .mechanism of biosensor .equipment used in biosensor .All the information contain about biosensor present in this presentation .its really informative .
A biosensor is an analytical device containing an immobilized biological material (enzyme, antibody, nucleic acid, hormone, organelle or whole cell) which can specifically interact with an analyte and produce physical, chemical or electrical signals that can be measured. An analyte is a compound (e.g. glucose, urea, drug, pesticide) whose concentration has to be measured.
Biosensor .now what is biosensor .mechanism of biosensor .equipment used in biosensor .All the information contain about biosensor present in this presentation .its really informative .
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
A sensor that integrates a biological element with a physiochemical transducer to produce an electronic signal proportional to a single analyte which is then conveyed to a detector.
Biosensor is the Talk of The Day. It made possible, the conversion of yesteryear's cumbersome experiments to an easier, faster all the while improving its sensitivity and specificity. This article will help you to gain an acquaintance about it, its properties, etc.
Biosenser are now a days a very helpful device which have various application in the field of medical in this presentation i described about biosensors and their types major application of biosensors
A Descriptive Review over the field of Biosensors has been given here; its origin history events; its working principle; its classification based on various parameters; applications and future scope
Biosensors: General Principles and ApplicationsBhatt Eshfaq
A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.
A biosensor is an independently integrated receptor transducer device, which is capable of providing selective quantitative or semi-quantitative analytical information using a biological recognition element.(IUPAC recommendations 1999)
Professor Leland c Clark junior (1918-2005) is called the father of biosensor. The inventor of the Clark electrode, a device used for measuring oxygen in blood, water and other liquids.
Biosensors play a part in the field of environmental quality, medicine and industry mainly by identifying material and the degree of concentration present.
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research.
This ppt is about biosensors. A brief Introduction to biosensors, history of Biosensors, working of biosensors, usage of biosensors application of biosensors in medical and other fields
A sensor that integrates a biological element with a physiochemical transducer to produce an electronic signal proportional to a single analyte which is then conveyed to a detector.
Biosensor is the Talk of The Day. It made possible, the conversion of yesteryear's cumbersome experiments to an easier, faster all the while improving its sensitivity and specificity. This article will help you to gain an acquaintance about it, its properties, etc.
Biosenser are now a days a very helpful device which have various application in the field of medical in this presentation i described about biosensors and their types major application of biosensors
A Descriptive Review over the field of Biosensors has been given here; its origin history events; its working principle; its classification based on various parameters; applications and future scope
Biosensors: General Principles and ApplicationsBhatt Eshfaq
A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.
A biosensor is an independently integrated receptor transducer device, which is capable of providing selective quantitative or semi-quantitative analytical information using a biological recognition element.(IUPAC recommendations 1999)
Professor Leland c Clark junior (1918-2005) is called the father of biosensor. The inventor of the Clark electrode, a device used for measuring oxygen in blood, water and other liquids.
Biosensors play a part in the field of environmental quality, medicine and industry mainly by identifying material and the degree of concentration present.
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research.
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research.
Biotechnology is challenging subject to teach and understand also..its a very interesting subject in pharmacy..all the power point is made as per your syllabus with point to point discussion.
thank you
This a short and efficient presentation On Biosensor for giving presentation in the upcoming seminar....
This could be more edited further for future purposes......
Contact: arnabguptakabiraj@gmail.com
This is for the beginners level giving presentation for the first time....
A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.The sensitive biological element, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material or biomimetic component that interacts with, binds with, or recognizes the analyte under study. The biologically sensitive elements can also be created by biological engineering. The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. The biosensor reader device connects with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way.[5] This sometimes accounts for the most expensive part of the sensor device, however it is possible to generate a user friendly display that includes transducer and sensitive element (holographic sensor). The readers are usually custom-designed and manufactured to suit the different working principles of biosensors.
QMS SOP [QUALITY MANAGEMENT SYSTEM - STANDARD OPERATING PROCEDURE]Nabeela Moosakutty
Standard Operating Procedure (SOP)
A Standard Operating Procedure (SOP) is a set of written
instructions that documents routine or repetitive activity followed by
an organization.
The development and use of SOPs are an integral part of a
successful quality system as it provides individuals with the information
to perform a job properly, and facilitates consistency in the quality and
integrity of a product or end-result. To ensure quality control, all
procedures are standardized, So SOPs are integral part of Quality
assurance process.
Purpose
SOPs detail the regularly recurring work processes that are to be
conducted or followed within an organization. They document the way
activities are to be performed to facilitate consistent conformance to
technical and quality system requirements and to support data quality.
They may describe, for example, fundamental programmatic actions and
technical actions such as analytical processes, and processes for
maintaining, calibrating, and using equipment. SOPs are intended to be
specific to the organization or facility whose activities are described and
assist that organization to maintain their quality control and quality
assurance processes and ensure compliance with governmental
regulations.
If not written correctly, SOPs are of limited value. In addition, the
best written SOPs will fail if they are not followed. Therefore, the use of
SOPs needs to be reviewed and re-enforced by management, preferably the
direct supervisor. Current copies of the SOPs also need to be readily
accessible for reference in the work areas of those individuals actually
performing the activity, either in hard copy or electronic format, otherwise
SOPs serve little purpose.
SOP-Benefits
a) The development and use of SOPs minimizes variation and promotes
quality.
b) SOPs can indicate compliance with organizational and governmental
requirements through detailed work instructions and can be used as
apart of a personnel training program.
c) It minimizes opportunities form is communication and can address
safety concerns. SOP-Writing Styles
SOPs should be written in a concise, step-by-step, easy-to-read format.
Information should not be overly complicated.
SOP Process
a) Preparation
The organization should have a procedure in place for
determining what procedures or processes need to be documented. Those
SOPs should then be written by individuals knowledgeable with the
activity and the organization's internal structure. These individuals
are essentially subject-matter experts who actually perform the work
or use the process.
SOPs should be written with sufficient detail so that someone with
limited experience with or knowledge of the procedure, but with a basic
understanding, can successfully reproduce the procedure when
unsupervised
b) Review and Approval
SOPs should be reviewed (that is, validated) by one or more
individuals with appropriate training and experience with the process.
Introduction
The pericardium is a fibrous sac that encloses the heart and great vessels. It keeps the heart in a stable location in the mediastinum, facilitates its movements, and separates it from the lungs and other mediastinal structures. It also supports physiological cardiac function.
Structure and Function
The pericardium consists of two layers: the fibrous and the serous. The fibrous pericardium is a conical-shaped sac. Its apex is fused with the roots of the great vessels at the base of the heart. Its broad base overlies the central fibrous area of the diaphragm with which it is fused. Weak sterno-pericardial ligaments connect the anterior aspect of the fibrous pericardium to the sternum. The serous pericardium is a layer of serosa that lines the fibrous pericardium (parietal layer), which is reflected around the roots of the great vessels to cover the entire surface of the heart (visceral layer). Between the parietal and visceral layers is a potential space that may be filled with a small amount of fluid. The part of the visceral layer that covers the heart, but not the great vessels is called the epicardium.
As the serous pericardium reflects off various cardiac structures, it forms two sinuses: the transverse sinus and the oblique sinus. The oblique sinus is a cul-de-sac extending superiorly from the inferior vena cava between the two left pulmonary veins on one side and the two right pulmonary veins on the other. Its anterior wall is formed by the posterior wall of the left atrium, between the four pulmonary veins. The oblique sinus provides expansion space for the left atrium. The transverse sinus is open at both ends and formed by the reflection of visceral serosal pericardium from the posterior aspects of the aortic and pulmonary trunks over to the anterior aspect of the atrium. Thus, a finger in the transverse sinus will pass behind the aortic and pulmonary trunks, in front of the superior vena cava on the right, and the left atrial appendage on the left.
The pericardial sac positions the heart in the mediastinum and limits its motion while providing a lubricated slippery surface for the heart to beat inside and the lungs to move outside. The pericardium prevents the excessive dilatation of the heart, and in pathological states, it can limit the overfilling of the heart, which would result in low cardiac output. It also influences the pressure-volume relationships of cardiac chambers by providing limited space for the heart as a whole. The pericardium also equalizes hydrostatic, inertial, and gravitational forces maintain the geometry of the left ventricle, and acts as a mechanical barrier to infection.
Pericarditis
Inflammation of the pericardium is called pericarditis. Its origin can be infectious, immunologic, metabolic, neoplastic, traumatic, or idiopathic. A myocardial infarction can also cause localized pericarditis of the area overlying the infarct.
Heart Anatomy
Location
Outlook
Components:
Layers
Chambers
Valves
Great vessels
Small brief Anatomy of the heart
Your heart is located between your lungs in the middle of your chest, behind and slightly to the left of your breastbone (sternum). A double-layered membrane called the pericardium surrounds your heart like a sac. The outer layer of the pericardium surrounds the roots of your heart’s major blood vessels and is attached by ligaments to your spinal column, diaphragm, and other parts of your body. The inner layer of the pericardium is attached to the heart muscle. A coating of fluid separates the two layers of membrane, letting the heart move as it beats.
Your heart has 4 chambers. The upper chambers are called the left and right atria, and the lower chambers are called the left and right ventricles. A wall of muscle called the septum separates the left and right atria and the left and right ventricles. The left ventricle is the largest and strongest chamber in your heart. The left ventricle’s chamber walls are only about a half-inch thick, but they have enough force to push blood through the aortic valve and into your body.
The Heart Valves
Four valves regulate blood flow through your heart:
The tricuspid valve regulates blood flow between the right atrium and right ventricle.
The pulmonary valve controls blood flow from the right ventricle into the pulmonary arteries, which carry blood to your lungs to pick up oxygen.
The mitral valve lets oxygen-rich blood from your lungs pass from the left atrium into the left ventricle.
The aortic valve opens the way for oxygen-rich blood to pass from the left ventricle into the aorta, your body’s largest artery.
The Conduction System
Electrical impulses from your heart muscle (the myocardium) cause your heart to contract. This electrical signal begins in the sinoatrial (SA) node, located at the top of the right atrium. The SA node is sometimes called the heart’s “natural pacemaker.” An electrical impulse from this natural pacemaker travels through the muscle fibers of the atria and ventricles, causing them to contract. Although the SA node sends electrical impulses at a certain rate, your heart rate may still change depending on physical demands, stress, or hormonal factors.
The Circulatory System
The heart and circulatory system make up your cardiovascular system. Your heart works as a pump that pushes blood to the organs, tissues, and cells of your body. Blood delivers oxygen and nutrients to every cell and removes the carbon dioxide and waste products made by those cells. Blood is carried from your heart to the rest of your body through a complex network of arteries, arterioles, and capillaries. Blood is returned to your heart through venules and veins. If all the vessels of this network in your body were laid end-to-end, they would extend for about 60,000 miles (more than 96,500 kilometers), which is far enough to circle the earth more than twice
Emulsions
Definition
These are homogenous, transparent and thermodynamically stable dispersion of water and oil stabilized by surfactant and co-surfactants
Consists of globules less than 0.1 μm in diameter
Types
Oil dispersed in water (o/w) - oil fraction low
Water dispersed in oil (w/o) - water fraction low
Bicontinuous (amount of oil and water are same)
Advantages
Thermodynamically stable, long shelf life
Potential reservoir of lipophilic or hydrophilic drug
Enhance the absorption and permeation of drugs through biological membranes
Increased solubility and stability of drugs
Ease and economical scale-up
Greater effect at lower concentration
Enhances the bioavailability of poorly soluble drugs
Theories of microemulsion
Interfacial or mixed film theory
Microemulsions are formed spontaneously due to formation of complex film at the interface by a mixture of surfactant and co-surfactant, As a result of which the interfacial tension reduces
Solubilization theory
Microemulsions are considered to be thermodynamically stable solutions of water swollen (w/o) or oil swollen (o/w) spherical micelles
Thermodynamic theory
The free energy of microemulsion formation is dependent on the role of surfactant in lowering the surface tension at the interface and increasing the entropy of the system
Multiple emulsions are complex polydispersed systems where both oil in water and water in oil emulsion exists simultaneously which are stabilized by lipophilic and hydrophilic surfactants respectively
The ratio of these surfactants is important in achieving stable multiple emulsions
They are also known as “Double emulsion” or “emulsion-within-emulsion”
Types
Oil-in-water-in-oil (O/W/O)
An o/w emulsion is dispersed in an oil continuous phase
Water-in-oil-in-water (W/O/W)
a w/o emulsion is dispersed in a water-continuous phase
MONOMOLECULAR ADSORPTION THEORY
MULTIMOLECULAR ADSORPTION THEORY
SOLID PARTICLE ADSORPTION THEORY
ELECTRICAL DOUBLE LAYER THEORY
ORIENTED WEDGE THEORY
Surfactants adsorb at the oil-water interface and form a monomolecular film
This film rapidly envelopes the droplets
They are very compact, elastic, flexible, strong and cannot be easily broken
For getting better stable emulsions combination of surfactants [surfactant blend] are used rather than a single one
The surfactant blend consists of both water soluble and oil soluble surfactants in order to approach the interface from aqueous and oil phase sides
At interface the surfactant blend interact to form a complex and condense a monomolecular film
Ex: A combination of Sodium cetyl sulfate (hydrophilic) and Cholesterol (lipophilic) forms a close packed complex film at the interface that produces an excellent emulsion
Dispersion system
suspensions
interfacial properties of suspensions
zeta potential
Sedimentation parameters
Settling in suspension
Formulation of suspension
Preparation of suspension
factors affecting protein drug binding
significance of protein binding
drug related factors
protein related factors
drug interactions
patient related factors
Controlled drug delivery system part 2
mechanism and different approaches of controlled drug delivery system
diffusion-controlled drug delivery
dissolution controlled drug delivery
ion-exchange resin system
Introduction, Definitions, Advantages and Disadvantages, Selection of drug candidates for designing controlled drug release systems and rationale biological and medical rationale
5th B.Pharm Pharmaceutical Jurisprudence
Import of Drugs: Classes of drugs and cosmetics prohibited from import, import license and registration certificate, import under license or permit, Offenses and Penalties
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
5. DEFINITION
Self-contained integrated device that is
capable of providing specific qualitative or
semi-quantitative analytical information using
a biological recognition element which is in
direct-spatial contact with a transduction
element. (IUPAC,1998)
A sensor that integrates a biological element
with a physiochemical transducer to produce
an electronic signal proportional to a single
analyte which is then conveyed to a detector
6. • What Is a Biosensor?
• Biosensor = bioreceptor + transducer
• The bioreceptor is a biomolecule that recognizes the target analyte whereas the
transducer converts the recognition event into a measurable signal.
• Enzyme is a Bioreceptor
Need ofbiosensor
• Diagnostic Market
• Clinical Testing
• clinical testing is one of the biggest diagnostic markets
• clinical testing products market in excess of 4000 million US$ in the 1990s
Specificity
• With biosensors, it is possible to measure specific analytes with
great accuracy.
Speed
• analyte tracers or catalytic products can be directly and instantaneously
measured
Simplicity
• receptor and transducer are integrated into one single sensor& the measurement of
target analytes without using reagents is possible
Continuous monitoring capability
• Biosensors regenerate and reuse the immobilized biological recognition
element
7. Simply any device that has specific biochemical
reactions to detect chemical compounds in
biological samples
20. SIGNAL
(How do you know there was a detection?)
Common Signalling Principles
Optical
Electrical
Electromechanical
Thermal
Magnetic
Pressure
Often the detector is immobilized on a solid support/sensor (The
immobilisation permits repeated use of the costlyBiological
Molecule.)
Specific recognition?
21. WORKING PRINCIPLE
Analyte diffuses from the solution
to the surface of the Biosensor
Analyte reacts specifically &
efficiently with the Biological
Component of the Biosensor
This reaction changes the physico-
chemical properties of the Transducer
surface
This leads to a change in the
optical/electronic properties of
the Transducer Surface
The change in the optical/electronic
properties is measured/converted
into electrical signal, which is
detected
22.
23. BASIC CHARACTERESTICS
LINEARITY - Should be High – For the
detection of High Substrate Concentration.
SENSITIVITY - Value of Electrode Response
per Substrate Concentration.
SELECTIVITY - Chemical Interference must be
minimised for obtaining Correct Result.
RESPONSE TIME – Time necessary for having
95% of the Response.
24. ADVANTAGES
Highly Specific
Independent of Factors like stirring, pH, etc.
Linear response, Tiny & Biocompatible
Easy to Use, Durable
Require only Small Sample Volume
Rapid, Accurate, Stable & Sterilizable
31. Optical Biosensors.
Colorimetric for colour - Measures change in
LightAdsorption.
Photometric for Light Intensity - Detects the
Photon output.
Resonant Biosensors.
An Acoustic Wave Transducer is coupledwith
Bioelement.
Measures the change in Resonant Frequency.
32. Physical Biosensor
Physical biosensors are the most fundamental as well as
broadly used sensors.
Any detecting device that offers reaction to the physical
possessions of the medium was named as a physical
biosensor.
The physical biosensors are classified into two types namely
piezoelectric biosensor and thermometric biosensor.
Piezoelectric Biosensors.
Uses Gold - To detect specific angle at which ȇ waves are
emitted when the substance is exposed to laser
light/crystals like quartz, which vibrates under the
influence of an electric field
Change in Frequency ᾶ Mass ofAbsorbed material
33. Calorimetric / Thermal Detection Biosensors.
Uses Absorption / Production of Heat.
Total heat produced/absorbed is ᾶ Molar
Enthalpy/Total No. of molecules in the rn.
Temp. measured by Enzyme Thermistors.
Advantages:
• No need of Frequent recalibration.
• Insensitive to the Optical & Electrochemical
Properties of the sample.
Uses:
Detection of: (1) Pesticides .
(2) Pathogenic Bacteria.
34. There are various types of biological reactions which are
connected with the invention of heat, and this makes the
base of thermometric biosensors. These sensors are usually
named as thermal biosensors
Thermometric-biosensor is used to
measure or estimate the serum
cholesterol. As cholesterol obtains
oxidized through the enzyme
cholesterol oxidize, then the heat
will be produced which can be
calculated. Similarly, assessments
of glucose, urea, uric acid, and
penicillin G can be done with these
biosensors.
35. Ion Sensitive Biosensors.
Are semiconductor FETs with ion-
sensitive surface.
Surface Electrical Potential changes
when the ions & semiconductors
interact.
Measures the Change in
Potential.
Uses:
o pH Detection.
36. Electrochemical Biosensors.
Underlying Principle – Many chem.rns produce or
consume ions or ȇs causing some change in the
elctrical properties of the solution that can be
sensed out & used as a measuring parameter.
Uses:
Detection of :
o Hybridized DNA
o DNA- binding Drugs &
o Glucose Concentration.
37.
38. Conductometric Sensors.
Measures Electrical Conductance/Resistance of
the solution
Conductance Measurements have relatively Low
Sensitivity.
Electrical Field is generated using
sinusoidal(ac) voltage, which helps in
minimizing undesirable
effects like:
i. Faradaic processes.
ii. Double layer charging &
iii. Concentration polarization.
39. Amperometric Biosensors.
High Sensitivity Biosensor.
Detects electro active species present in
the biological test samples.
Measured Parameter – Current.
40. Potentiometric Sensors.
Working Principle – When ramp voltage is applied
to an electrode in solution, a current flow
occurs because of electrochemical reactions.
Measured Parameter – Oxidation / reduction
Potential of an Electrochemical rn.
41. 4. Impedimetric Biosensors
The EIS (Electrochemical impedance
spectroscopy) is a responsive indicator for
a broad range of physical as well as
chemical properties.
A rising trend towards the expansion of
Impedimetric-biosensors is being presently
observed.
The techniques of Impedimetric have been
executed to differentiate the invention of
the biosensors as well as to examine the
catalyzed responses of enzymes lectins,
nucleic acids, receptors, whole cells, and
antibodies.
42.
43. OpticalBiosensor
• The optical fibers allow
detection of analytes on the
basis of absorption,
fluorescence or light
scattering. optical
biosensors have the
advantages of to in vivo
applications and allowing
multiple analytes to be
detected by using different
monitoring wavelengths.
Fluorescence-based optical Biosensor
46. FoodAnalysis.
Study of Biomolecules & their Interaction.
Drug Development.
Crime Detection.
Medical Diagnosis (Clin&Lab).
Environmental Field Monitoring.
Quality Control.
Industrial Process Control.
Detection Systems for Biological Warfare Agents.
Manf. Of Pharmaceuticals & Replacement organs.
47. BIOSENSOR FOR AGRICULTURAL
& FOOD INDUSTRY
o Detection of viral, fungal, bacterial diseases of
plants.
o In food industry, detection of total microbes &
food quantification in soft drinks.
o To determine the freshness of other fish, beef &
other food items.
o Makes Bacteria GLOW by OPTICAL Biosensor
48. ApplicationsofBiosensor
continue…….
• Biosensor can be used for many analytical
problems, ranging from detection of industrial
toxins and food contamination to monitoring the
density of microbes in an industry and medical
diagnostics.
• Biosensor for medical diagnostics.
• Biosensor for agriculture and food industry.
• Biosensor for environment monitoring.
• Toxicology tests using biosensor.
• Biosensor for general industry.
• Biosensor for military and defense industry.
49. Common healthcare checking
Metabolites Measurement
Screening for sickness
Insulin treatment
Clinical psychotherapy &
diagnosis of disease
In Military
Agricultural, and Veterinary
applications
Drug improvement, offense
detection
Processing & monitoring in
Industrial
Ecological pollution control
50. The DNA capture
element instrument- for
hereditary diseases
Glucometer- for
measurement of
glucose in blood.
51. Pregnancy Test
•Detects the hCG protein in
urine.
•Interpretation and data
analysis performed by the
user.
Infectious Disease Biosensor
•Data analysis and
interpretation performed by
a microprocessor