The document discusses biosensors, which combine a biological component with a physicochemical detector. It defines biosensors as analytical devices used to detect analytes. The basic components of a biosensor are a biological recognition element, transducer to convert the biological response into an electrical signal, and a detector. Common types of biosensors include electrochemical, optical, piezoelectric, and ion-sensitive biosensors. The document also outlines the basic principles, components, characteristics, and applications of biosensors.
A presentation on biosensors and its application,all datas r mainly collected from google search,and from some books by or teachers. Hope it will help you...leave your rply,, :)
Biosensors, Types of Biosensors, Applications of Biosensors, Nanotechnology, Nanobiosensors, Components of Biosensor, Working of Biosensor, Principle of Biosensor, Examples of Biosensor, Advantages of Biosensor, Disadvantages of Biosensor, Limitations of Biosensor, Features of a Biosensor, Calorimetric Biosensors, Potentiometric Biosensors, Acoustic Wave Biosensors, Amperometric Biosensors, Optical Biosensors, Examples of a Nanobiosensor, Lab on a chip,
Applications of Lab on a chip, Glucose Biosensor
With increase in potential for bioterrorism, there is a great demand to detect the bio agents in the atmosphere in a quick, reliable and accurate method. Biosensor is a analytical device which uses enzymes, immunosystems, tissues that converts biological response into electrical, thermal or optical signals. Biosensor is an efficient and cost effective device which is most widely used for various day to day applications. Biosensor consists of two components: first the “sensing element” and second is the “transducers”. Sensing element may be either enzymes, antibodies, DNA, tissues or whole cells which then transduces the biochemical reaction into electrical signals. Basic advantage of biosensor is the use of nanomaterials, micro fluidics and transducer on a single chip. Biosensors have found its application in fermentation, food industry, diagnosis, imaging, DNA sequencing and biodefense. Development of nanotechnology leads to the development of macro and micro sensors which is small and sensitive.
Biosensors have become more popular with biochemistry and analytical chemistry. Biosensors are used to detect pollutants, microbial load, control parameters and metabolites. Leland C Clark is the father of biosensor who invented the glucose biosensor to determine the glucose level in the sample. Clark entrapped glucose oxidase in a dialysis membrane and placed within a oxygen electrode. DNA sensor has been included in the family of biosensors which can be used for disease diagnosis. Biosensors are fabricated using nanotechnology, these devices helps use to analyze in a quick and accurately.
A Biosensor is a device for the detection of an analyte that combines a biological component with a physio-chemical detector component.
Download: https://www.topicsforseminar.com/2014/10/biosensors-ppt.html
A presentation on biosensors and its application,all datas r mainly collected from google search,and from some books by or teachers. Hope it will help you...leave your rply,, :)
Biosensors, Types of Biosensors, Applications of Biosensors, Nanotechnology, Nanobiosensors, Components of Biosensor, Working of Biosensor, Principle of Biosensor, Examples of Biosensor, Advantages of Biosensor, Disadvantages of Biosensor, Limitations of Biosensor, Features of a Biosensor, Calorimetric Biosensors, Potentiometric Biosensors, Acoustic Wave Biosensors, Amperometric Biosensors, Optical Biosensors, Examples of a Nanobiosensor, Lab on a chip,
Applications of Lab on a chip, Glucose Biosensor
With increase in potential for bioterrorism, there is a great demand to detect the bio agents in the atmosphere in a quick, reliable and accurate method. Biosensor is a analytical device which uses enzymes, immunosystems, tissues that converts biological response into electrical, thermal or optical signals. Biosensor is an efficient and cost effective device which is most widely used for various day to day applications. Biosensor consists of two components: first the “sensing element” and second is the “transducers”. Sensing element may be either enzymes, antibodies, DNA, tissues or whole cells which then transduces the biochemical reaction into electrical signals. Basic advantage of biosensor is the use of nanomaterials, micro fluidics and transducer on a single chip. Biosensors have found its application in fermentation, food industry, diagnosis, imaging, DNA sequencing and biodefense. Development of nanotechnology leads to the development of macro and micro sensors which is small and sensitive.
Biosensors have become more popular with biochemistry and analytical chemistry. Biosensors are used to detect pollutants, microbial load, control parameters and metabolites. Leland C Clark is the father of biosensor who invented the glucose biosensor to determine the glucose level in the sample. Clark entrapped glucose oxidase in a dialysis membrane and placed within a oxygen electrode. DNA sensor has been included in the family of biosensors which can be used for disease diagnosis. Biosensors are fabricated using nanotechnology, these devices helps use to analyze in a quick and accurately.
A Biosensor is a device for the detection of an analyte that combines a biological component with a physio-chemical detector component.
Download: https://www.topicsforseminar.com/2014/10/biosensors-ppt.html
BIOSENSOR, PHARMACEUTICAL BIOTECHNOLOGY, B PHARAM, 6TH SEM
Basic components of Biosensor
Working of Biosensor
Types of Biosensor
Electrochemical biosensor
Optical biosensor
Thermal biosensor
Resonant biosensor
Ion-sensitive biosensor
Applications of Biosensor
Nano sensors
sensing device
Father of the Biosensor
components of BIOSENSOR
BASIC PRINCIPLE OF BIOSENSOR
BIO-ELEMENT
TRANSDUCER
DETECTOR
RESPONSE FROM BIO-ELEMENT
IDEAL BIOSENSOR
BASIC CHARACTERESTICS
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Biosensors are the analytical device that are used to measure the concentration of analye , these type of biosensors are made with conjugation of enzymes as a biological eliment to quantify a (bio)chemical substance / analyte are reffered to as Enzyme-probe Biosensors .
Biosensors are of many types but focusing on Enzyme biosensors there are 4 main types which are briefly described in this power point presentation .
BIOSENSOR, PHARMACEUTICAL BIOTECHNOLOGY, B PHARAM, 6TH SEM
Basic components of Biosensor
Working of Biosensor
Types of Biosensor
Electrochemical biosensor
Optical biosensor
Thermal biosensor
Resonant biosensor
Ion-sensitive biosensor
Applications of Biosensor
Nano sensors
sensing device
Father of the Biosensor
components of BIOSENSOR
BASIC PRINCIPLE OF BIOSENSOR
BIO-ELEMENT
TRANSDUCER
DETECTOR
RESPONSE FROM BIO-ELEMENT
IDEAL BIOSENSOR
BASIC CHARACTERESTICS
Introduction :
Antibiotics are antimicrobial agents produced naturally by other microbes (usually fungi or bacteria)
The first antibiotic was discovered in 1896 by Ernest Duchesne and in 1928 "rediscovered" by Alexander Fleming from the filamentous fungus Penicilium notatum.
The antibiotic substance, named penicillin, was not purified until the 1940s (by Florey and Chain), just in time to be used at the end of the second world war.
Penicillin was the first important commercial product produced by an aerobic, submerged fermentation
The material describes components of industrial fermentation media with their respective metabolic importance for the industrial microbes. it also addresses industrial scale sterilization methods.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
Biosensors are the analytical device that are used to measure the concentration of analye , these type of biosensors are made with conjugation of enzymes as a biological eliment to quantify a (bio)chemical substance / analyte are reffered to as Enzyme-probe Biosensors .
Biosensors are of many types but focusing on Enzyme biosensors there are 4 main types which are briefly described in this power point presentation .
Dr.S.Karthikumar
Assistant Professor, Dept. of Biotechnology
Kamaraj College of Engineering and Technology
Madurai, Tamilnadu, INDIA
skarthikumar@gmail.com
Dr.S.Karthikumar
Asst. Prof., Dept. of Biotechnology
Kamaraj College of Engineering and Technology
S.P.G.C.Nagar, Virudhunagar, Tamilnadu, India
skarthikumar@gmail.com
Biosensor and its Applications.
Biosensors are analytical devices that combine a biological component with a physicochemical detector to provide specific and sensitive detection of target analytes.
Importance: Biosensors have revolutionized the way we detect and monitor various substances, from biomarkers to environmental pollutants.
Biosensor is an leading Biological technology now. It is an application of Biotechnology. It makes laboratory tests more fast and easy to carry out. It is cost effective, more accurate precise, and have less errors.
Biosensers are the integrated receptor transducer device, which is capable of providing selective quantitative or semi-quantitative analytic information using a biological recognition element.
Analytical device.
Contains Biological or Biological derived recognition element to detect specific bio-analyte a transducer to convert a biological signal into an electrical signal.
A biosensor is an analytical device containing an immobilized biological material (enzyme, antibody, nucleic acid, hormone, organelle or whole cell) which can specifically interact with an analyte and produce physical, chemical or electrical signals that can be measured. An analyte is a compound (e.g. glucose, urea, drug, pesticide) whose concentration has to be measured.
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research.
Austin Journal of Biosensors & Bioelectronics is an open access, peer reviewed, scholarly journal dedicated to publish articles related to original and novel fundamental research in the field of Biomarkers Research.
The aim of the journal is to provide a platform for research scholars, scientists and other professionals to find most original research in the field Biosensors & Bioelectronics.
Austin Journal of Biosensors & Bioelectronics accepts original research articles, review articles, case reports and short communication on all the aspects of Biosensors & Bioelectronics and its Research
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2. • Father of Biosensor – Professor Leland C
Clark
• The term ‘biosensor’ is short for “biological
sensor”
• Analytical device used for the detection of
an analyte, that combines a biological
component with a physicochemical detector.
• It is a device incoporating a biological
sensing element either intimately connected
to or integrated within a transducer
3. • Recognition based on affinity between complementary
structures like:
Enzyme-substrate, antibody-antigen and receptor-hormone
complex
• Selectivity and specificity depend on biological recognition
systems connected to a suitable transducer.
4. • It converts a response into an electrical signal
• It detects, records, and transmits information regarding a
physiological change or process
• It determines the presence and concentration of a specific
substance in an test solution
• Main features of biosensors are
stability
cost
sensitivity
reproducibility
5. Basic principle of biosensor
• Basic principle involved in three element :-
o First biological recognition element which highly specific towards
the biological material analytes produces
o Second transducers detect and transduces signal from biological
target – receptor molecule to electrical signal which is due to
reaction occur
o Third after transduction signal from biological to electrical signal
where its amplification is necessary and takes place and read out in
detector after processing the values are displayed for monitor and
controlling the system
8. Ist component – Biological element
• The component used to bind the target molecule
• Must be highly specific, stable under storage conditions and
immobilized
• A variety of substance may be used as bioelement in
biosensor
Microorganism
Tissue, Cell, Organelle
Nucleic acid
Enzyme, Enzyme component
Receptor
Antibody
Plant proteins or lectins
9. • Functions;
To interact specifically with a target compound i.e the
compound to be detected
It must be capable of detecting the presence of a target
compound in the test solution
The ability of a bio-element to interact with target compound
(specificity) in the basis of biosensor
10. 2nd component – Physiochemical
Transducer
• Acts as an interface, measuring the physical change that
occurs with the reaction at the bioreceptor then transforming
that energy into measureable electrical output
11. 3rd component - Detector
• Signals from the transducer are passed to a microprocessor
where they are amplified and analyzed.
• The data is then converted to concentration units and
transferred to a display or / and data storage device
12.
13. • The block diagram of biosensor include
three segments namely sensor, transducer
and associated electrons.
• In the first segment the sensor is a
responsive biological part
• Second segment is the detector part that
changes the resulting signal from the contact
of the analyte and for the results it displays
in an accesible way
• The final section comprises of an amplifier
which is known as signal conditioning circuit,
the display unit as well as the processor.
14. Basic principle of Biosensor
• The biological material is immobilized and a contact is made
between the immobilized biological material and the
transducer
• The analyte binds to the biological material to form a bound
analyte which inturn produces the electronic response that
can be measured
• Sometimes the analyte is converted to a product which could
be associated with the release of heat, gas(oxygen), electrons,
hydrogen ions.
• The transducer then converts the product linked changes into
electrical signals which can be amplified and measured
15. Working principle of Biosensor
Analyte diffuses from the solution to the surface of the
biosensor
Analyte reacts specifically and efficiently with the biological
component of the biosensor
This reaction changes the physicochemical properties of the
transducer surface
This leads to a change in the optical/ electronic properties of
the transducer surface
The changes in the optical/ electronic properties is measured/
converted into electrical signal, which is detected
16. • Usually a specific enzyme or preferred biological material is
deactivated by some of the usual methods and the
deactivated biological material is in near contact to the
transducer
• The analyte connects to the biological object to shape a clear
analyte which in turn gives the electronic reaction that can be
calculated
• In some examples, the analyte is changed to a device which
may be connected to the discharge of gas, heat, electron ions
or hydrogen ions
• In this the transducer can alter the device linked converts into
electrical signals which can be changed and calculated
17.
18. How does they work…??
• Basically it involve the quantitative analysis of various
substances by converting their biological actions into
measurable signals
• Generally the performance of the biosensors is mostly
dependent on the specificity and sensitivity of the biological
reaction, besides the stability of the enzyme
19. Ideal Biosensor
1. The output signal must be relevant to measurement
enviornment
2. The functional surface must be compatible with the
transducer
3. High specificity and sensitivity (low interference)
4. Sufficient sensitivity and resolution
5. Surface accuracy and repeatability
6. Sufficient speed of response
7. Sufficient dynamic range
8. Intensivity to enviornmental interference or their effects
must be compensated
20. Basic characteristics of Biosensor
1. LINEARITY – linearity of the sensor should be high for the
detection of high substrate concentration
2. SENSITIVITY – Value of the electrode response per substrate
concentration
3. SELECTIVITY – Chemicals interference must be minimised for
obtaining the correct result
4. RESPONSE TIME – Time necessary for having 95% of the
response
23. Calorimetric / Thermal Detection Biosensors
Uses Absorption / Production of heat
Total heat produced / absorbed is α Molar Enthalpy / Total
No. of molecules in the run
Tem. Measured by Enzyme Thermistors
Advantages
• No need of frequent recalibration
• Intensitive to the optical and electrochemical properties of
the sample
Uses
• Detection of : (1) Pesticides
(2) Pathogenic bacteria
24. Ion Sensitive Biosensors
Surface electrical potential changes when the ion and
semiconductor interact
Measures the change in potential
Uses
Detection of pH
Electrochemical Biosensors
Many chemical runs produce or consume ions or electrons causing
some change in the electrical properties of the solution that can be
sensed out and used as a measuring parameter
Uses
Detection of :- (1) hybridized DNA
(2) DNA binding drugs
(3) glucose concentration
25. Optical Biosensors
Colorimetric for colour – Measure change in light adsorption
Photometric for light intensity – Detects the photon input
Resonant Biosensors
• An Acoustic Wave Transducer is coupled with bioelement
• Measures the change in Resonant Frequency
Piezoelectric Biosensors
• Uses gold – To detect specific angle at which electron waves
are emitted when the substance is exposed to laser light/
crystals like quartz, which vibrates under the influence of an
electric field
• Change in frequency α Mass of absorbed material
26. Conductimetric Sensors
Measures electrical conductance / resistance of the solution
Measurement has low sensitivity
Electrical voltage is generated using sinusoidal (ac) voltage,
which helps in minimizing undesirable effects like :
i) Faradiac processes
ii) Double layer charging
iii) Concentration polarization
Amperometric Biosensors
High sensitivity
Detects the electroactive species present in the biological test
samples
Measured parameter – current
27. Potentiometric Sensors
Working principle – when voltage is applied to an electrode in
solution a current flow occurs because of electrochemical
reactions
Measured parameter – oxidation / reduction potential of an
electrochemical run
28. Applications
• Food analysis
• Study of biomolecules and their interaction
• Drug development
• Crime detection
• Medical diagnosis (both clinical and laboratory use)
• Enviornmental field monitoring
• Quality control
• Industrial process control
• Detection systems for biological warefare agents
• Manufacturing of pharmaceuticals and replacement organs