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AUTOMATIC FASCICLE LENGTH ESTIMATION ON MUSCLE ULTRASOUND
IMAGES WITH AN ORIENTATION-SENSITIVE SEGMENTATION
ABSTRACT
Goal:Fascicle length obtained by ultrasoundimaging is one of the crucial muscle
architecture parameters forunderstanding the contraction mechanics and pathologicalconditions
of muscles. However, the lack of a reliable automaticmeasurement method restricts the
application of fascicle lengthfor the analysis of muscle function, as frame-by-frame
manualmeasurement is time-consuming. In this study, we propose anautomatic measurement
method to preclude the influence ofnon-fascicle components on the estimation of fascicle length
byusing motion estimation of fascicle structures.
Methods:Themethod starts with image segmentation using the cohesiveness offascicle
orientation as a feature, obtaining the fascicle change bytracking manually marked points on the
fascicular path with the Lucas–Kanade optical flow algorithm applied on the segmentedimage.
Results:The performance of this method was evaluated onultrasound images of the
gastronomies obtained from 7 healthysubjects (34.4 ± 5.0 years). Waveform similarity between
themanual and dynamic measurements was assessed by calculatingthe overall similarity with the
coefficient of multiple correlations(CMC). In vivo experiments demonstrated that fascicle
trackingwith the orientation-sensitive segmentation (CMC = 0.97 ± 0.01)was more consistent
with the manual measurements than existingautomatic methods (CMC = 0.87 ± 0.10).
Conclusion:Our methodwas robust to the interference of non-fascicle
components,resulting in a more reliable measurement of fascicle length.
Significance:The proposed method may facilitate furtherresearch and applications related
to real-time architecturalchange of muscles.

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  • 1. AUTOMATIC FASCICLE LENGTH ESTIMATION ON MUSCLE ULTRASOUND IMAGES WITH AN ORIENTATION-SENSITIVE SEGMENTATION ABSTRACT Goal:Fascicle length obtained by ultrasoundimaging is one of the crucial muscle architecture parameters forunderstanding the contraction mechanics and pathologicalconditions of muscles. However, the lack of a reliable automaticmeasurement method restricts the application of fascicle lengthfor the analysis of muscle function, as frame-by-frame manualmeasurement is time-consuming. In this study, we propose anautomatic measurement method to preclude the influence ofnon-fascicle components on the estimation of fascicle length byusing motion estimation of fascicle structures. Methods:Themethod starts with image segmentation using the cohesiveness offascicle orientation as a feature, obtaining the fascicle change bytracking manually marked points on the fascicular path with the Lucas–Kanade optical flow algorithm applied on the segmentedimage. Results:The performance of this method was evaluated onultrasound images of the gastronomies obtained from 7 healthysubjects (34.4 ± 5.0 years). Waveform similarity between themanual and dynamic measurements was assessed by calculatingthe overall similarity with the coefficient of multiple correlations(CMC). In vivo experiments demonstrated that fascicle trackingwith the orientation-sensitive segmentation (CMC = 0.97 ± 0.01)was more consistent with the manual measurements than existingautomatic methods (CMC = 0.87 ± 0.10). Conclusion:Our methodwas robust to the interference of non-fascicle components,resulting in a more reliable measurement of fascicle length. Significance:The proposed method may facilitate furtherresearch and applications related to real-time architecturalchange of muscles.