The Colosseum in Rome was the largest amphitheater ever built in the Roman Empire, seating 50,000 spectators. Constructed in the 1st century AD under Emperors Vespasian and Titus, it hosted gladiatorial fights and executions. Throughout its history, it was damaged by fires, earthquakes, and later used as a quarry for materials like marble and stone for other buildings such as St. Peter's Basilica and the defenses of the Tiber River.