The document summarizes screening models for Alzheimer's disease. It describes two in vitro methods - inhibition of acetylcholine-esterase activity in rat striatum and inhibition of butyrylcholine-esterase activity in human serum. It also describes two in vivo methods - the step-down test and scopolamine-induced amnesia in mice. The step-down test measures learning and memory in rats using an elevated platform and electric shock. The scopolamine-induced amnesia test measures the ability of drugs to reverse memory deficits caused by the antimuscarinic scopolamine in mice. The document provides details on the procedures and evaluations of these screening methods.
Screening Methods for behavioural and muscle Coordinationpradnya Jagtap
Screening Methods for behavioural and muscle Coordination
A. Motor activity and behaviour
1. Method of intermittent observation
2.Open field test
3.Hole board test
4.Combined open field test
B.Test for muscle coordination
1.Inclined plane method
2.Chimny test
3.Grip strength
4.Rotarod method
This file includes the general introduction to Alzheimer's, histopathology and Pharmacological treatment of Alzheimer's, preclinical screening models used in Alzheimer's. I hope this file may useful to life science students
BRM has developed proprietary rat strains which are translatable models for the characterization of type 1 and type 2 diabetes as well as immune-related diseases. Its technical competencies include design and conducting studies in autoimmunity (type 1 diabetes, lupus, Rheumatoid Arthritis and Multiple Sclerosis), metabolic disease (type 2 diabetes, metabolic syndrome, obesity, dyslipidemia, liver and kidney function), virology, neurodegenerative diseases, diabetic complications (retinopathy, neuropathy, nephropathy, and encephalopathy including Alzheimer’s disease) specialized animal model development and the production and care of multiple species.
Screening Methods for behavioural and muscle Coordinationpradnya Jagtap
Screening Methods for behavioural and muscle Coordination
A. Motor activity and behaviour
1. Method of intermittent observation
2.Open field test
3.Hole board test
4.Combined open field test
B.Test for muscle coordination
1.Inclined plane method
2.Chimny test
3.Grip strength
4.Rotarod method
This file includes the general introduction to Alzheimer's, histopathology and Pharmacological treatment of Alzheimer's, preclinical screening models used in Alzheimer's. I hope this file may useful to life science students
BRM has developed proprietary rat strains which are translatable models for the characterization of type 1 and type 2 diabetes as well as immune-related diseases. Its technical competencies include design and conducting studies in autoimmunity (type 1 diabetes, lupus, Rheumatoid Arthritis and Multiple Sclerosis), metabolic disease (type 2 diabetes, metabolic syndrome, obesity, dyslipidemia, liver and kidney function), virology, neurodegenerative diseases, diabetic complications (retinopathy, neuropathy, nephropathy, and encephalopathy including Alzheimer’s disease) specialized animal model development and the production and care of multiple species.
A compiled Power point presentation on "Antipsychotic drugs" suitable for Undergraduate level medical students and also PG students in the subject of Pharmacology.
Radha_Chafle_303_ALZHEIMER’S DISEASE AND PARKINSON DISEASE.pptxRadhaChafle1
The presentation is about the alzheimer's disease and Parkinson Disease. In this presentation, various screening models are given for both of the diseases.
Assignment on Preclinical Screening of ImmunomodulatorsDeepak Kumar
Assignment on Preclinical screening of new substances for the pharmacological activity using in vivo, in vitro, and other possible animal alternative models
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?
Alzheimer models
1. INTEGRAL UNIVERSITY,LUCKNOW
SESSION: 2016-2017
SEMINAR PRESENTATION
ON
Screening models for Alzheimer’s disease
Presented by : Under the guidance of :
Mohammad Muztaba Dr. Anuradha Mishra
M.Pharm, Pharmacology Dr. Badruddeen
1st year
2. Chronic neurodegenerative disorder
Largest cause of dementia in the elderly
Fourth leading cause of mortality
About 27 million patients worldwide
Symptoms of AD is:
Progressive dementia:
Loss of memory
Cognitive decline
Impairment of judgment
Changes in personality
Prevalence:
5.4% in Western Europe and 1.9% in India.
Alzheimer’s disease (AD)
3. Histopathology of AD
Accumulation of protein aggregates:
Intracellular: neurofibrillary tangles consisting mostly of Tau, a
microtubule-associated protein.
Extracellular: deposition of amyloid (Aβ42) plaques.
This leads to progressive cortical cell loss and cortical atrophy.
4. The Changing Brain in Alzheimer’s Disease:
Pet Scan of Normal Brain Alzheimer’s Disease Brain
Preclinical AD
Signs of AD are first noticed in the entorhinal
cortex, then proceed to the hippocampus:
Affected regions begin to shrink as nerve
cells die.
Changes can begin 10-20 years before
symptoms appears.
Memory loss is the first sign of AD.
5. Goals for the Treatment of
Alzheimer’s
Improve memory
Improve functional status
Improve behavioral symptoms
Slow progression
Delay or prevent onset
6. MOA Cholinesterase Inhibitors
NMDA-
Receptor
Antagonist
Drug Donepezil Galantamine Rivastigmine Memantine
Indication
Mild-moderate AD;
severe AD
Mild-moderate AD
Mild-moderate
AD
Moderate-
severe AD
Initial
dose
Tablet:
5 mg qd
Tablet/oral
solution:
4 mg bid
ER capsule: 8 mg
qd
Capsule/oral
solution: 1.5
mg bid
Patch: 4.6 mg
qd
Tablet/oral
solution: 5 mg
qd
Maximal
dose
Tablet:
10 mg qd
Tablet/oral
solution:
12 mg bid
ER capsule: 24
mg qd
Capsule/oral
solution: 6 mg
bid
Patch: 9.5 mg
qd
Tablet/oral
solution: 10 mg
bid
Pharmacologic Treatments for Alzheimer’s Disease (AD)
Source: National Institute on Aging. Alzheimer’s disease medications. November 2008. NIH Publication No.
08-3431. Available at: http://www.nia.nih.gov/Alzheimers/Publications/medicationsfs.htm. Accessed July 24,
2009.
MOA = mechanism of action; ER = extended-release; NMDA = N-methyl-D-aspartate
9. 1. Inhibition of acetylcholine-esterase activity in
rat straitum:
Purpose and Rationale:
To screen drugs for inhibition of acetylcholine-esterase
activity.
As it is generally accepted that the physiological role of
AChE is rapid hydrolysis and inactivation of Ach.
Thus, inhibitor of AChE show marked cholinomimetic
effects in cholinergically –innervated effectors organs.
Recent studies have suggested that AChE inhibitors may
be beneficial for the treatment of AD.
10. PROCEDURE:
1. Reagents:
a. 0.05 M Phosphate buffer, ph 7.2
b. Substrate in buffer(198 mg acetylthiocholine
chloride)
c. DTNB in buffer (19.8 mg 5,5-dithiobisnitrobenzoic
acid )(0.5mM)
d. A 2mM stock sol. of test drug is made up in a
suitable solvent and q.s to volume with 0.5mM DTNB.
Drugs are serially diluted(1:10) such that the final
conc. in cuvette is 10-4 M and screened for the activity.
11. 2. TISSUE PREPARATION:
RATS ARE DECAPITATED
BRAINS ARE RAPIDLY REMOVED, CORPORA
SRAITA DISSECTED FREE
WEIGHED AND HOMOGENIZED IN 19 VOLUMES
(Approx. 7 mg protein/ml)OF 0.05 M NaH2 PO4,ph 7.2.
25μl ALIQUOT OF THIS SUSPENSION IS ADDED TO 1ml
OF THE VEHICLE AND VARIOUS CONCENTRATION OF
TEST DRUG ARE PREPARED.
REINCUBATED FOR 10 MIN AT 37οC.
12. 3. EVALUATION:
• For IC 50 determinations : Substrate conc. is 10 mM
diluted 1:2 in an assay yielding a final conc. of 5 mM.
• DTNB conc. is 0.5 mM yielding 0.25 mM final conc.
% Inhibition= slope control – slope drug × 100
slope control
• IC 50 values are calculated from log- probit analysis.
13. 2. INHIBITION OF BUTYRYLCHOLINE-
ESTERASE ACTIVITY IN HUMAN SERUM:
Purpose and Rationale:
This method is used in conjuction with the
acetylcholine-esterase assay to determine the enzyme
selectivity of various cholinesterase inhibitors.
Butyrylcholine-esterase preferentially hydrolyses
butyrylcholine.
This enzyme is found in highest amount in serum,but
its physiological role is not known.
Ethopropazine and Tetra-Isopropyl
Pyrophosphoramide
(ISO-OMPA) are selective inhibitors of
butyrylcholinesterase.
14. PROCEDURE:
1. Reagents:
a. 0.05 M Phosphate buffer, ph 7.2
b. Substrate in buffer(225.8 mg s- butyrylthiocholine
chloride)
c. DTNB in buffer (19.8 mg 5,5-dithiobisnitrobenzoic
acid) (0.5mM)
d. A 2mM stock sol. of test drug is made up in a
suitable solvent and q.s to volume with 0.5mM
DTNB. Drugs are serially diluted(1:10) such that
determined from the inhibitory activity of
subsequent conc.
15. 2. ENZYME PREPARATION:
A VIAL OF LYOPHILIZED HUMAN SERUM
RECONSTITUTED IN 3ML OF DISTIILED WATER
25 ml ALIQUOT OF THIS SUSPENSION
ADDED TO 1ml OF THE VEHICLE & VARIOUS CONC.
OF TEST DRUG ARE PREPARED
PRE-INCUBATE FOR 10MIN AT 37οC.
16. 3. EVALUATION:
• For IC 50 determinations : Substrate conc. is 10 mM
diluted 1:2 in an assay yielding a final conc. of 5 mM.
• DTNB conc. is 0.5 mM yielding 0.25 mM final conc.
% Inhibition= slope control – slope drug × 100
slope control
• IC 50 values are calculated from log- probit analysis.
18. SOME OF THE IN-VIVO METHODS ARE:
Inhibitory(passive) avoidance:
• Step-down
• Step-through
• Two-compartment test
• Up-hill avoidance
• Trail-to-criteria inhibitory avoidance
• Scopolamine-induced amnesia in mice
• Memory impairment by basal forebrain lesions in rat
• Ischemia-induced amnesia in gerbis
• Cognitive deficit on chronic low dose MPTP-treated
monkeys
Active avoidance:
• Runway avoidance
19. 1. STEP-DOWN:
Purpose and Rationale:
An animal(mouse or rat) in an open spends most of
the time close to the walls and in corners.
When placed on an elevated platform in the centre of
a rectangular compartment,it steps down almost
immediately to the floor to explore the encloser and
to approach the wall.
This technique is employed in different
modifications.
20. PROCEDURE:
REQUIREMENTS:
a) Mice or rats of either sex are used.
b) A rectangular box (50×50) with electrifiable
grid floor 35 fits over the block.
c) Grid floor is connected to shock device.
21. A typical paradigm consists of :
a) Familiarization
b) Learning
c) Retension test
22. a) FAMILIARIZATION:
Animal is placed on the platform
Released after raising the cylinder
Latency to descend is measured
After 10 seconds of expolaration,it is
returned to the home cage
23. B) LEARNING:
Immediately the animal has descended from
the platform
An avoidable shock is applied(foot shock:
50Hz: 1.5 mA ; 1 sec)
Animal is returned to the home cage
24. C) RETENSION TEST:
24 hrs after the learning trail
The animal is again placed on the platform
step-down latency is measured
The test is finished when the animal steps
down or remain on the platform(cut-off time:
60 sec)
25. EVALUATION :
• The time of descent during the learning phase and
the time during the retention test is measured .
• A prolongation of the step-down latency is defined
as learning.
26. 2. SCOPOLAMINE –INDUCED AMNESIA IN
MICE:
Purpose and Rationale:
The administration of anti- muscuranic agent
scopolamine to young human volunteers produces
transient memory deficits.
Similarly, scopolamine impairs memory retention
when given to mice.
The ability of different cholinergic drug agonist to
reverse the amnesic affects of scopolamine is now
well documented in animal and human volunteers.
27. PROCEDURE:
The scopolamine test is performed in groups of 10 male
NMRI mice weighing 26-32 g in one trail.
Five min after i.p administration of 3mg/kg of
scopolamine hydrobromide.
Each mouse is placed invidually in bright part of two
chambered apparatus.
After brief orientation period,mouse enters the second
or darker chamber.
Once inside second chamber ,the doors are closed to
avoid escape.
A 1 mA, 1-sec foot shock is applied through grid floor.
The mouse then return to home cage.
28. • 24 hrs later , testing is performed by placing the
animal again in the bright chamber.
• The latency in entering the dark chamber within 5
min test session is measured electronically.
• Whereas, untreated control animals enter the darker
chamber in second trail with the latency of about
250sec.
• Treatment with scopolamine reduces the latency to
50 sec.
• Test compounds are administered 90 min before
training.
• The prolonged latency indicates that animal
remembers that it has been punished and,therefore,
avoids darker chamber.
29. EVALUATION:
• After treatment with various doses of test drug
latencies obtained were expressed as % latencies .
• In some cases, straight dose-response curve is
obtained whereas with other drugs inverse U-shaped
dose responses are observed.