The document summarizes amino acid metabolism and the metabolism of ammonia. It discusses:
1) Amino acids exist in an amino acid pool that increases after eating and decreases during fasting. Plasma amino acid levels range from 4-8 mg/dl.
2) Amino acids are used to synthesize proteins, peptides, and other biological molecules or undergo catabolism where the amino group is removed through transamination or deamination reactions.
3) Ammonia produced from amino acid catabolism is transported to the liver and converted to urea by the urea cycle. Elevated ammonia can be toxic and cause hyperammonemia.
Catabolism of Phenylalanine and Tyrosine | Disorders Of Tyrosine Metabolismkiransharma204
This PPT contains topic related to Catabolism of Phenylalanine and Tyrosine, Disorders Of Tyrosine Metabolism and metabolic disorders like Phenyketonuria, Albinism, Alkaptonuria and Tyrosinemia.
Books referred: https://www.amazon.in/s?k=satyanarayan+biochemistry&i=stripbooks&crid=2UMKA76J0R8WC&sprefix=satya%2Cstripbooks%2C456&ref=nb_sb_ss_i_2_5
Urea Cycle | Energetics of Urea Cycle | Regulation of Urea Cycle | Metabolic ...kiransharma204
This PPT contains topics related to Urea Cycle, Energetics of Urea Cycle, Regulation of Urea Cycle, Metabolic disorder of Urea Cycle & Clinical Importance.
Books referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?crid=3FLX88MWT4Y30&dchild=1&keywords=satyanarayan+biochemistry&qid=1591701828&s=books&sprefix=satyanarayan+%2Cstripbooks%2C387&sr=1-1
This PPT is on Amino acid metabolism. And the topics covered under this ppt are Transamination, deamination
Book referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?dchild=1&qid=1591608419&refinements=p_27%3AU+Satyanarayana&s=books&sr=1-1
Catabolism of Phenylalanine and Tyrosine | Disorders Of Tyrosine Metabolismkiransharma204
This PPT contains topic related to Catabolism of Phenylalanine and Tyrosine, Disorders Of Tyrosine Metabolism and metabolic disorders like Phenyketonuria, Albinism, Alkaptonuria and Tyrosinemia.
Books referred: https://www.amazon.in/s?k=satyanarayan+biochemistry&i=stripbooks&crid=2UMKA76J0R8WC&sprefix=satya%2Cstripbooks%2C456&ref=nb_sb_ss_i_2_5
Urea Cycle | Energetics of Urea Cycle | Regulation of Urea Cycle | Metabolic ...kiransharma204
This PPT contains topics related to Urea Cycle, Energetics of Urea Cycle, Regulation of Urea Cycle, Metabolic disorder of Urea Cycle & Clinical Importance.
Books referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?crid=3FLX88MWT4Y30&dchild=1&keywords=satyanarayan+biochemistry&qid=1591701828&s=books&sprefix=satyanarayan+%2Cstripbooks%2C387&sr=1-1
This PPT is on Amino acid metabolism. And the topics covered under this ppt are Transamination, deamination
Book referred: https://www.amazon.in/Biochemistry-2019-Satyanarayana-Satyanarayana-Author/dp/B07WGHCTKZ/ref=sr_1_1?dchild=1&qid=1591608419&refinements=p_27%3AU+Satyanarayana&s=books&sr=1-1
De novo synthesis of fatty acids (Biosynthesis of fatty acids)Ashok Katta
Synthesis of fatty acids in the body. Detailed pathway for de novo synthesis of fatty acids in the body including its energetic and regulation. also cover Multienzyme complex
De novo synthesis of fatty acids (Biosynthesis of fatty acids)Ashok Katta
Synthesis of fatty acids in the body. Detailed pathway for de novo synthesis of fatty acids in the body including its energetic and regulation. also cover Multienzyme complex
Metabolism of amino acids (general metabolism)Ashok Katta
Metabolism of amino acids (general metabolism).
Part - I of amino acid metabolism.
This presentation covers Transamination, deamination, formation and Transport of Ammoniaand etc.
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
Overview of amino acid anabolism and catabolism and fate of ammonia in amino acid metabolism. This is targeted for MBBS, MD, BDS and general Biochemistry students
designed for undergraduate level teaching of nitrogen metabolism in biochemistry. this is first in the series of three lectures. ideal for MBBS level teaching
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
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Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
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- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
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These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
• Evidence-based strategies to address health misinformation effectively
• Building trust with communities online and offline
• Equipping health professionals to address questions, concerns and health misinformation
• Assessing risk and mitigating harm from adverse health narratives in communities, health workforce and health system
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
2. The amount of free amino acids distributed
throughout the body is called amino acid pool.
It tends to increase in the fed state and tends to
decrease in the post absorptive state
Plasma amino acids ranges between 4 –8 mg/dl
3. Synthesis of non
essential amino
acids
Dietary protein
Breakdown of
tissue proteins
Amino acids
pool
Sources of amino acids Fate of amino acids
Formation of Structural
protein (eg: tissue proteins)
Biosynthesis of peptide
hormones, haemoglobin,
myoglobin & enzymes
Synthesis of biological
important peptides (eg:
Glutathione)
Biosynthesis of NPN
substances (eg: Urea, uric acid,
creatine, creatinine)
5. It evaluate the relationship between the Nitrogen -intake
(in the form of protein) & Nitrogen -excretion
Two situations are possible
Positive Nitrogen balance
Negative Nitrogen balance
9. • All the catabolic pathway of amino acids (removal of amino
group) involves
Transamination,
Deamination reactions
to form α-keto acid and Ammonia.
• Further carbon skeleton either enters carbohydrate
(glucogenic) or lipid metabolisms (ketogenic)
• Ammonia is transported from the muscle to liver & converted
into urea than excreted.
12. Significance of transamination reactions
• This can provides the amino groups from many different a.a’s
into one common product L-glutamate
• L-GLU is the only amino acid whose α-amino group can be
directly removed at high rate by oxidative deamination.
• L-GLU can be used as an amino group donor in the synthesis
of non essential amino acids
• All amino acids can be transaminated except lysine,
threonine, proline and OH- proline.
13. Pyruvate
+
L-Glutamate
L- Alanine
+
α- Ketoglutarate
ALT / SGPT
PLP
Serum glutamate pyruvate transaminase (SGPT) /
Alanine amino transferase (ALT)
- Specific for liver diseases and also increases in acute
hepatitis, hepatic Jaundice
16. • Removal of amino group from α-amino acid in the form
of ammonia with formation of α-keto acid.
• Occurs in liver and kidney.
• Deamination occurs in two ways:
A. Oxidative deamination.
B. Non oxidative deamination
17. Oxidative deamination
• Removal of ammonia from the amino acids with the link of
oxidation process
• It is catalyzed by enzymes Glutamate dehydrogenase and
L & D-amino acid oxidase.
18. Glutamate dehydrogenase(GLDH)
(In hepatocytes)
GTP , ATP & NADH
-
GLDH can use either NAD+ or NADP+ as the acceptor of
reducing equivalents
GLDH increased in cases of liver disease (hepatocellular
damage )
+
GDP , ADP
20. Non-oxidative deamination
• Removal of ammonia from the amino acids without link of
oxidation process
• Dehydratase enzyme deaminates amino acids containing OH-
group & it needs PLP as coenzyme.
Serine + H2O Pyruvate + NH4
+
Serine dehydratase
Threonine dehydratase
Threonine α- ketoglutarate + NH4
+
22. From amino acids via
Transamination &
deamination
Degradation of
biogenic amines
Ammonia
Formation and Metabolic fate of ammonia in the body
Converted to urea (urea cycle)
Synthesis of non essential
amino acids
Formation of Purines &
Pyrimidines
Maintains the acid base
balance via NH4
+ ions
From the amino
group of purines &
Pyrimidines
By the action of intestinal
bacteria (urease) on urea
Synthesis of Glutamine
Formation of amino sugars
23. Transport of Ammonia
• Ammonia is transported from muscle to liver in two transport
forms glutamine and alanine but not as free ammonia.
• I. Glutamine is a major transport & temporary storage form of
ammonia
Glutamate synthetase
Ammonia +Glutamate +ATP Glutamine +ADP+ Pi
Glutaminase
Glutamine + H2O Glutamate + NH4
+
Liver
24. Glutaminase
• Two isoenzymes of mitochondrial glutaminase
Hepatic glutaminase - increases high protein intake
Renal glutaminase - increases in metabolic acidosis
• Excretion of ammonia into urine produced by the action of R-
glutaminase in renal tubular cells regulates acid base balance.
• Production of ammonia from intracellular renal glutamine
increase in metabolic acidosis And decreases in metabolic
alkalosis
25. • II. Alanine is important NH3
transporter from muscle to
liver by glucose – alanine
cycle (in starvation).
• Glutamate can transfer its α-
amino group to pyruvate by
the action of ALT to form
alanine.
• Liver promptly removes the
ammonia from the portal
blood.
27. • The concentration of ammonia in blood : 40 to 70 μgm/dl,
AMMONIA TOXICITY:
• Elevation of NH3 in blood is found to be toxic to the body.
Hyper ammonemia:
a) Acquired hyper ammonemia
• Usually the result of cirrhosis of the liver
• Leads to reducing the synthesis of urea.
28. b) Inherited hyper ammonemia :
results from genetic defects in the urea cycle enzymes.
Ammonia intoxication is characterized by:
– A peculiar flapping tremor.
– Slurring of speech.
– Blurring of vision.
– And in severe cases coma and death
due to increased NH3 concentration in blood & brain.
29. • High levels of ammonia leads to increased conc. of glutamine
– acts as an osmotically active solute in brain astrocytes,
• Triggers an uptake of water into the astrocytes to maintain
osmotic balance, leading to swelling of the cells – coma
• Terminal stages of ammonia intoxication characterized by
cerebral edema and increased cranial pressure
30. Treatment of hyper ammonemia
• Dietary Protein restriction
• Increased Arginine in diet, bypasses Arginosuccinase
defect
• Drugs like benzoate and phenyl acetate,
• Hemodialysis
31. Believe In Yourself And
All That You Are.
Know That There Is
Something Inside You
That Is Greater Than
Any Obstacle.
32. Next class
DISPOSAL OF AMMONIA
Ammonia has been disposed as Urea
by urea cycle
Thank you
Have a nice day to all