This document discusses dermatology and skin diseases. It covers the structure and functions of skin, common causes of skin diseases including infectious (viral, bacterial, mycotic, parasitic) and non-infectious causes. It describes common skin lesions and diseases such as dermatitis. Dermatitis is inflammation of the skin that can be caused by bacteria, fungi, parasites, chemicals, allergies or other sources. Symptoms include redness, swelling, blisters and itching. Treatment involves identifying and removing the cause, using antibiotics for bacterial infections, anti-fungals, anti-parasitics or anti-allergy medications as needed.
Mange is among the common illnesses that many dog owners are concerned about. People usually get tensed and do not know how to handle such situations. I present you this Presentation that speaks about Mange, a skin disease caused by tiny parasite mites on dogs. You can refer to this to know what Mange is all about, the types of Mange, the causes, the symptoms and a few easy ways to deal with it. Mange, if left untreated can be fatal. Therefore, timely knowledge and treatment about this disease is important. You can also add your views in the comments below.
For more information about Mange, you can refer to the following links:
http://www.vet-organics.com/types-of-mange-in-dogs/
http://pets.webmd.com/dogs/mange-dogs-canine-scabies
Diagnosis and Treatment of Canine Pyodermaupstatevet
Ed Jazic, DVM, DACVD
The prevalence of Canine Pyoderma is increasing very quickly and the clinical condition can present in a variety of ways. It is a common secondary manifestation of a variety of clinical conditions like allergic skin diseases, endocrinopathies, autoimmune skin diseases, and keratinization disorders. An efficient and correct diagnosis is essential as is proper therapy in the face of ever-increasing development of Canine Methicillin-Resistant Staphylococcal Pyoderma.
Mange is among the common illnesses that many dog owners are concerned about. People usually get tensed and do not know how to handle such situations. I present you this Presentation that speaks about Mange, a skin disease caused by tiny parasite mites on dogs. You can refer to this to know what Mange is all about, the types of Mange, the causes, the symptoms and a few easy ways to deal with it. Mange, if left untreated can be fatal. Therefore, timely knowledge and treatment about this disease is important. You can also add your views in the comments below.
For more information about Mange, you can refer to the following links:
http://www.vet-organics.com/types-of-mange-in-dogs/
http://pets.webmd.com/dogs/mange-dogs-canine-scabies
Diagnosis and Treatment of Canine Pyodermaupstatevet
Ed Jazic, DVM, DACVD
The prevalence of Canine Pyoderma is increasing very quickly and the clinical condition can present in a variety of ways. It is a common secondary manifestation of a variety of clinical conditions like allergic skin diseases, endocrinopathies, autoimmune skin diseases, and keratinization disorders. An efficient and correct diagnosis is essential as is proper therapy in the face of ever-increasing development of Canine Methicillin-Resistant Staphylococcal Pyoderma.
The reason for the occurrence in heifers is still unanswered
It may be because of stress of calving particularly with udder edema and hormonal changes lead to activation of BHM virus and development of signs (Gibbs, 1984 ; Kemp et al., 2008 ; Martin, 1973; Sankaram and Kotayya, 1977).
The clinical condition was usually sporadic in occurrence within first two months of lactation in the heifers of first calving preferably in winter season with hind quarter infection (Mouli, 1992; Sharma et al., 1998 ; Sharma and Singh, 2006).
The reason for the occurrence in heifers is still unanswered
It may be because of stress of calving particularly with udder edema and hormonal changes lead to activation of BHM virus and development of signs (Gibbs, 1984 ; Kemp et al., 2008 ; Martin, 1973; Sankaram and Kotayya, 1977).
The clinical condition was usually sporadic in occurrence within first two months of lactation in the heifers of first calving preferably in winter season with hind quarter infection (Mouli, 1992; Sharma et al., 1998 ; Sharma and Singh, 2006).
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
2. Dermatology (Skin Diseases)
• The skin is a covering for the whole
body so it is a mirror that reflects
many systemic and local diseases.
• The incidence of skin diseases in
animals is high.
• Many of the skin diseases are
contagious therefore quick and
accurate diagnosis is important in
their control and eradication.
3. Function o f the skin
(1) Protection from light, organisms and
mechanical.
(2) Immunologic function.
(3) Vitamin D synthesis.
(4) Heat regulation.
(5) Secretary organ by sweating : e.g. wastes,
urea.
(6 ) Water reserve.
(7) Sensory organs.
(8 ) Reflection of internal feelings
4. Causes o f skin diseases:
[1] Infectious:
(1) Viral:
1) Contagious erythema.
2) Ulcerative dermatitis in sheep.
3) Lumpy skin diseases (in cattle).
4) Pox.
5) Vesicular papular dermatitis (in
horse).
5. (2) Bacterial:
1) Bacterial dermatitis. 2)
Tuberculosis. 3) Foot rot.
(3) Mycotic:
1) Ring worm.
2) Epizootic lymphangitis.
3) Spirocheta.
(4) Parasitic:
1) Protozoa e.g.
Leishmaniasis.
2) Arthropodes as mange.
3) Helminthes as filariasis.
6. [2] Non infectious:
(1) Nutritional deficiency of zinc, cobalt,
iodine and copper.
(2 ) Chemical injury as acids, alkalies and
gases.
(3) Histamine production: urticaria.
(4) Lesions due to severe abrasion or
friction.
(5) Disturbance in circulation such as
erythema.
(6 ) Distribution in nervous tissue “itching”.
7. (7) Internal diseases (constipation, nephritis).
(8 ) Neoplasms (wastes, papilloma,
carcinoma).
(9) Congenital defects (alopecia).
(10) Over feeding of carbohydrate without
enough exercise.
(11) Traumatic (wounds).
(12) Thermal, excessive heat, sun rays.
(13) Long administration of some drugs as
arsenic, mercury, serum causing skin rashes.
(14) Endocrine disorders.
8. Scientific Term used in Skin
Diseases
[1] Primary lesions (less than 1 cm
in size):
(1) Macule: It is a flat circumscribed discoloration o f
the skin or mucous membrane.
(2) Papule: A solid elevation of the skin or mucous
membrane extending deeper in the epidermis.
(3) Nodule: A solid elevation of the skin or mucous
membrane extending into the dermis (neoplastic or
inflammatory).
(4) Vesicle: A fluid filled superficial circumscribed
elevation of skin or mucous membrane.
9.
10. [2] Lesions more than 1 ml: Such as
cyst, tumor, granuloma, plaque.
[3] Primary lesions of varying sizes:
(1)Pustule: Vesicle of the skin or
mucous membrane which contain pus.
(2)Wheal: An irregularly shaped,
elevated transient lesion of the skin or
mucous membrane due to edema.
11.
12. [4] Secondary cutaneous lesions:
(1) Erosion: Shallow ulcer of the epidermis
doesn’t penetrate the entire epidermis.
(2) Erythema: A redness of skin due to
capillary congestion.
(3) Hyperkeratosis: An increased thickening
of the keratin layer of the epidermis (normal in
the foot pads, nose of dog and cat).
(4) Scab or crust: A collected dried exudate
on the surface of the epidermis adherent to
hairs.
13.
14. (5) Scale: A collection of excessive
keratin flacks upon the surface o f the
skin.
(6) Scar: A permenant depigmented area
o f fibrous tissue without hair growth.
(7) Parakeratosis: Retention of nuclei in
the keratin layer (stratum comium) with
absence of stratum granulosum
characterized by scale formation.
(8) Seborrhea: Over active disease of
sebaceous glands result in oilness, crust
or scales.
15.
16.
17.
18. Principle s of treatment of skin diseases:( 1)
Accurate diagnosis of the cause to choose the correct
topical or systemic treatment by obtaining complete
medical history and through medical examination.
(2) Removal of hair coat and debris to enable
topical application to come into contact with the
causative agent.
(3) In allergic diseases remove the causative
agent.
(4) In bacterial disease, sensitivity tests on
culture of the organism are advisable
19. (5) Treat the primary cause and prevent
secondary infection by:
1) Base of bacteriostatic ointment or dressing.
2) Administration of local anaesthetic
ointments to prevent further damage from
scratching.
3) Fluid therapy in case of fluid and
electrolytes losses.
4) Sulpher containing amino acids or zinc or
vitamin A facilitate the repair of skin tissues.
20. Drugs used for Treatment of Skin Diseases:
(1) Stimulants:
!These are compounds which have an action on the skin.
!They are powders, aqueous sol., suspensions or mineral oils.
!They applied externally on the skin lesion.
(2) Sedatives: They used to depress irritation and it may be:
1) Emollients:
1- Oils: such as olive, caster, cotton seed oil.
2- Fats: such as wool fat.
3- Wax: such as bees wax.
4- Hydrocarbon: as liquid paraffin, cod-liver oil and glycerin.
2) Sedative powders: As chalk, talk, zinc carbonate and kaolin.
(3) Astringent:
It coagulates protein forming a protective layer. Such as copper sulphate, zinc
sulphate and ferric chloride.
(4) Corticosteroid:
They inhibits fibroblasts and diminish the fibrosis of chronic inflammation, inhibit
vasodilatation and increase permeability and exudate formation of acute
inflammation.
21. (5) Counter irritant:
They are substances which applied to the skin to cause
local irritation and inflammation, e.g. iodine.
(6) Caustics:
They are agents which destroy tissue and excessive
granulation and superficial tumors (warts), ex.: Nitric acid,
caustic soda, phenol, mercury chloride.
22. (I) Diseases of the Epidermis
and Dermis
They include Dermatitis, Eczema,
Urticaria, Photosensitization, Pityriasis,
Parakeratosis and Hyperkeratosis.
•Dermatitis
•Definition:
•It is inflammation of the dermis and epidermis.
•Causes:
•(1) In all species:
•1) Mycotic dermatitis due to Dermatophilus congolensis in horses, cattle
and sheep.
•2) Staphylococcus aureus.
3) Ring worm.
25. (3) In sheep and goat:
1) Sheep pox.
2) Ulcerative dermatitis.
3) Fleece rot.
4) Ovine dermatitis.
5) Caprine idiopathic dermatitis.
(4 ) In horses:
1) Horse pox.
2) Canadian horse pox.
3) Viscular stomatitis.
4) Dermatophytes including ringworm.
Pathogenesis:
(1) Inflammation of deeper layers of the skin involving blood
vessels and lymphatics.
(2) It may be acute, chronic, suppurative, weeping ulcerative or
gangrenous.
(3) Increased thickness, temperature, pain and itching.
26.
27. Clinical findings:
(1) Symptoms start by erythema,
vesicles.
(2) Edema of the skin and
subcutaneous tissues.
(3) Healing stage or scab formation or
may be necrosis or gangrene of the
affected area in severe cases.
(4) Systemic reaction may occur when
the affected skin area is extensive.
28. (5 ) Spread o f infection to subcutaneous may result in
diffuse cellulitis. *
(6) Shock with peripheral circulatory failure may be present
in the early stages.
(7) Toxemia due to absorption of tissue breakdown.
Clinical pathology and diagnosis:
(1) Skin scraping or swabs for parasites or bacterial
examination.
(2) Cellular and sensitivity tests for bacteria.
(3) In allergic or parasitic states, accumulation of
eosinophils in the inflammed area.
29. •Treatment:
(1) Hygienic treatment:
1) Remove the injuries and harmful stimuli.
2) Remove the physical or chemical agent from
environment.
3) Supply balanced diet to repair nutritional deficiency (vit.
A, B, E, and D).
(2) Medical treatment:
•In infectious causes, identify the causative agent, make
sensitivity test (in bacterial infection) to select the specific
antibacterial drug. E.g. garamycin, terramycin ointement
applied on the skin lesion.
30. (2) Systemic:
1)Antihistaminic: It is recommended when tissue
destruction is extensive or the dermatitis is allergic in
origin.
2)2) Calcium preparations
3) Fluid therapy: If shock is present.
4) Parental antibiotic and anti-fungal agents:
5) Anaesthetic drugs:
•NB: If the lesions are extensive or secondary bacterial
invasion is likely to occur, parental antibiotic or antifungal
agents may be preferred to topical application.
NB: The use of vaccination as prophylaxis in viral and
bacterial dermatitis must not be neglected. Autogenous
vaccines may be most satisfactory in bacterial infections.