A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs). These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
ABO blood group system was decover by Karal landsteine
which contain A, B, and o antigen on the surface of BC, WBC,s platatelet and other body tissue cells except brain cell, and anti A, antiB and Anti Ab natural occuring antibodies in plasma of B,A, and O blood group individual respectively
A blood type (also called a blood group) is a classification of blood based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs). These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
ABO blood group system was decover by Karal landsteine
which contain A, B, and o antigen on the surface of BC, WBC,s platatelet and other body tissue cells except brain cell, and anti A, antiB and Anti Ab natural occuring antibodies in plasma of B,A, and O blood group individual respectively
The Compatibility can be determined by matching the different blood group systems, such as ABO and Rh system, and/or by directly testing for the presence of antibodies against a sample of donor tissues or blood.
The main purpose of this test is to distinguish the appearance of antibodies in the recipient against the red blood cells of the donor. These antibodies can be found on the surface of red blood cells of the donor after transfusion.
The Compatibility can be determined by matching the different blood group systems, such as ABO and Rh system, and/or by directly testing for the presence of antibodies against a sample of donor tissues or blood.
The main purpose of this test is to distinguish the appearance of antibodies in the recipient against the red blood cells of the donor. These antibodies can be found on the surface of red blood cells of the donor after transfusion.
• A blood group also called a Blood Type.
• Classification of blood is based on the presence or absence
of inherited antigenic substances on the surface of red blood
cells (RBCs).
• These antigens may be proteins, carbohydrates,
glycoproteins, or glycolipids, depending on the blood group
system.
A blood group also called a Blood Type
Classification of blood is based on the presence or absence of inherited antigenic substances on the surface of red blood cells (RBCs)
These antigens may be proteins, carbohydrates, glycoproteins, or glycolipids, depending on the blood group system.
The ABO blood group system is the most important blood type system (or blood group system) in human blood transfusion.
ABO blood types are also present in some other animals for example rodents and apes such as chimpanzees, bonobos and gorillas.
BLOOD GROUPING AND CROSS MATCHING.
overview of blood grouping
History
classification of blood typing
ABO SYTEM
INTRODUCTION ON ABO SYSTEM AND KARL LANSTIENER
LANSTEINER'S LAW
PRESENCE AND ABSENCE OF ANTIGEN AND ANTIBODIES
RH SYSTEM INCLUDING RH ANTIGEN AND POITIVE AND NEGATIVE BLOOD GROUPS
BLOOD GROUP COMPATABILITY
EXPLANATION ON CROSS MATCHING
TYPES OF CROSS MATCHING INCLUDING MAJOR AND MINOR
NOTE ON UNIVERSAL DONOR AND RECIPIENT
AGGLUTINATION
Anteriorly bordered by the lamina terminalis, with the anterior commissure above and the optic chiasm below.
Posteriorly bordered by interpeduncular fossa.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
4. Glossary
• Heterozygous
• 2 identical chromosomes with DIFFERENT
Gene types i.e. Dominant & Recessive
• Homozygous
• 2 identical chromosomes with the SAME
genes on each chromosome ie Dominant &
Dominant
5. Glossary
• Locus
• Location of a gene on a chromosome
• Allele
• Variation of a gene that produces an alternative
Phenotype
• Phenotype
• The result of expressing a gene ie Hair colour
• Gene
• A section of DNA made of bases that code for a
phenotype. ie eye colour
6. Glossary
• Sex Linked Chromosome
• Is either X or Y. Determines gender. Females
have XX, males have XY
• Autosomal Chromosome
• Pairs of chromosomes that have identical gene
location, and are the same in Male and
Females. Only the code within the gene varies.
7. Glossary
• Dominant Gene
• A strong gene that only needs one copy on one
chromosome to be expressed.
• Recessive gene
• Weak gene that needs a copy on each
autosomal chromosome (M&F), or on each
XX in women, or on the X in men, to be
expressed
9. History of Blood Groups and Blood Transfusions
•Experiments with blood transfusions
have been carried out for hundreds of
years. Many patients have died and it was
not until 1901, when the Austrian Karl
Landsteiner discovered human blood
groups, that blood transfusions became
safer.
• He found that mixing blood from two
individuals can lead to blood clumping.
The clumped RBCs can crack and cause
toxic reactions. This can be fatal.
http://nobelprize.org/medicine/educational/landsteiner/readmore.html
10. • Karl Landsteiner discovered that blood
clumping was an immunological reaction
which occurs when the receiver of a blood
transfusion has antibodies against the donor
blood cells.
•Karl Landsteiner's work made it possible to
determine blood types and thus paved the
way for blood transfusions to be carried out
safely. For this discovery he was awarded the
Nobel Prize in Physiology or Medicine in
1930.
History of Blood Groups and Blood
Transfusions (Cont.)
11. What is blood made up of?
An adult human has about 4–6 liters of
blood circulating in the body.
Blood consists of several types of cells
floating around in a fluid called plasma.
The red blood cells (RBCs) contain
haemoglobin, a protein that binds oxygen.
RBCs transport oxygen to, and remove
carbon dioxide from the tissues.
The white blood cells fight infection.
The platelets help the blood to clot, if you
get a wound for example.
The plasma contains salts and various
kinds of proteins.
12. •The differences in human blood are due to the
presence or absence of certain protein molecules
called antigens and antibodies.
•The antigens are located on the surface of the
RBCs and the antibodies are in the blood
plasma.
•Individuals have different types and
combinations of these molecules.
•The blood group you belong to depends on
what you have inherited from your parents.
What are the different blood groups?
13. • There are more than 20 genetically determined
blood group systems known today
• The AB0 and Rhesus (Rh) systems are the
most important ones used for blood transfusions.
• Not all blood groups are compatible with each
other. Mixing incompatible blood groups leads to
blood clumping or agglutination, which is
dangerous for individuals.
What are the different blood groups?
14. According to the ABO blood
typing system there are four
different kinds of blood types:
A, B, AB or O (null).
ABO blood grouping system
15. Blood group A
If you belong to the blood
group A, you have A
antigens on the surface of
your RBCs and B
antibodies in your blood
plasma.
Blood group B
If you belong to the blood
group B, you have B
antigens on the surface of
your RBCs and A
antibodies in your blood
plasma.
AB0 blood grouping system
16. Blood group AB
If you belong to the blood group
AB, you have both A and B
antigens on the surface of your
RBCs and no A or B antibodies
at all in your blood plasma.
Blood group O
If you belong to the blood group O
(null), you have neither A or B
antigens on the surface of your RBCs
but you have both A and B antibodies
in your blood plasma.
17. • The "A“ and "B" antigens are also produced
by some other plants and microorganisms.
Thus, individuals who do not recognize one or
more of these antigens as "self" will produce
antibodies against the plant or microbial
antigens.
• These antibodies will also react with human
antigens of the same kind whether introduced
via a blood transfusion or a tissue graft.
Why do individuals produce antibodies to
antigens they do not have?
18. • The ABO gene is autosomal (the gene is not on either sex
chromosomes)
• The ABO gene locus is located on the chromosome 9.
• Each person has two copies of genes coding for their ABO blood
group (one maternal and one paternal in origin)
• A and B blood groups are dominant over the O blood group
• A and B group genes are co-dominant
ABO inheritance and genetics
19. AUTOSOMAL
CHROMOSOME
Mustafa
Sara
one alleles from Mustafa and one
from Sara.
The alleles for Blood
group are in the same
place on the
chromosome 9. However
the genes have a
different code giving the
different blood group
A B
20. This meant that if a person inherited one A group gene and
one B group gene their red cells would possess both the A and
B blood group antigens.
These alleles were termed A ( which produced the A antigen ),
B (which produced the B antigen) and O (which was "non
functional"and produced no A or B antigen)
What do co-dominant genes mean?
23. The ABO blood groups
• The most important in assuring a safe blood transfusion.
• The table shows the four ABO phenotypes ("blood groups") present
in the human population and the genotypes that give rise to them.
Blood
Group
Antigens
on RBCs
Antibodies in Serum Genotypes
A A Anti-B AA or AO
B B Anti-A BB or BO
AB A and B Neither AB
O Neither Anti-A and anti-B OO
24. Why group A blood must never be
given to a group B person?
Giving someone blood from the wrong ABO
group could be fatal.
The anti-A antibodies in group B attack group
A cells and vice versa.
• Blood group O negative is a different story.
25. Well, it gets more complicated here, because there's
another antigen to be considered - the Rh antigen.
Some of us have it, some of us don't.
If it is present, the blood is RhD positive, if not it's RhD
negative.
So, for example, some people in group A will have it, and
will therefore be classed as A+ (or A positive).
While the ones that don't, are A- (or A negative).
And so it goes for groups B, AB and O.
The Rhesus (Rh) System
26. • Rh antigens are transmembrane proteins with loops
exposed at the surface of red blood cells.
• They appear to be used for the transport of carbon
dioxide and/or ammonia across the plasma membrane.
• They are named for the rhesus monkey in which they
were first discovered.
• RBCs that are "Rh positive" express the antigen
designated D.
• 85% of the population is RhD positive, the other 15% of
the population is running around with RhD negative
blood.
The Rhesus (Rh) System (Cont.)
28. According to above
blood grouping systems,
you can belong to either
of following 8 blood
groups:
Do you know which blood group you
belong to?
29. • A person with Rh- blood can develop Rh antibodies
in the blood plasma if he or she receives blood from a
person with Rh+ blood, whose Rh antigens can trigger
the production of Rh antibodies.
•A person with Rh+ blood can receive blood from a
person with Rh- blood without any problems.
30. Why is an Rh incompatibility so dangerous
when ABO incompatibility is not during
pregnancy?
• Most anti-A or anti-B antibodies are of the IgM
class (large molecules) and these do not cross the
placenta.
•In fact, an Rh−
/type O mother carrying an
Rh+
/type A, B, or AB foetus is resistant to
sensitisation to the Rh antigen.
•Her anti-A and anti-B antibodies destroy any foetal
cells that enter her blood before they can elicit anti-
Rh antibodies in her.
31. •This phenomenon has led to an effective
preventive measure to avoid Rh sensitisation.
•Shortly after each birth of an Rh+
baby, the
mother is given an injection of anti-Rh
antibodies (or Rhogam).
•These passively acquired antibodies destroy
any foetal cells that got into her circulation
before they can elicit an active immune
response in her.
Rh incompatibility during pregnancy (cont.)
32. The ABO Blood Group System
Laboratory Determination of the
ABO System
33. Several methods for testing the ABO group of an
individual exist. The most common method is:
Serology: This is a direct detection of the ABO
antigens. It is the main method used in blood
transfusion centres and hospital blood banks.
This form of testing involves two components:
a) Antibodies that are specific at detecting a
particular ABO antigen on RBCs.
b) Cells that are of a known ABO group that
are agglutinated by the naturally occurring
antibodies in the person's serum.
34. • Illustration of the forward and reverse
grouping reaction patterns of the ABO
groups using a blood group tile
http://www.bh.rmit.edu.au/mls/subjects/abo/resources/genetics1.htm
35. When RBCs carrying one or both antigens are exposed to the
corresponding antibodies, they agglutinate; that is, clump
together. People usually have antibodies against those red cell
antigens that they lack.
Human RBC before (left) and after (right) adding serum
containing anti-A antibodies. The agglutination reaction
reveals the presence of the A antigen on the surface of the
cells.
http://users.rcn.com/jkimball.ma.ultranet
/BiologyPages/B/BloodGroups.html
36. People with blood group O
are called "universal
donors" and people with
blood group AB are called
"universal receivers."
Blood transfusions – who can
receive blood from
whom?
38. Blood
Group
Antigens Antibodies Can give
blood to
Can
receive
blood from
AB A and B None AB AB, A, B, O
A A B A and AB A and O
B B A B and AB B and O
O None A and B AB, A, B, O O