protein chemistry

1. BBS153009

2. DOMAIN

3. DEFINITION
*Protein sequences and structures that
evolve, function, and exist independently
from the rest of the protein.
They often form functional
units, like metal
binding domains.

4. PYRUVATE KINASE
*Glycolytic Enzyme
*Regulating the flux from fructose
*An α/β-substrate binding domain
*An α/β-regulatory domain

5. UNITS OF PROTEIN
STRUCTURE

6. SECONDARY STRUCTURAL
CONTENT OF DOMAIN

7. SIZE
*From 36 residues in E-selectin to 692
residues in lipoxygenase-1

8. DOMAINS AS EVOLUTIONARY
MODULES
*Domains are the common material
used by nature to generate new
sequences; they can be thought of as
genetically mobile units, referred to
as 'modules'.

9. MULTIDOMAIN PROTEINS
*80% in metazoa, are multidomain proteins
*40% of prokaryotic proteins consist of multiple
domains
*65% eukaryotes have multi-domain proteins

11. DOMAINS OF UNKNOWN
FUNCTION
*A domain of unknown function (DUF) is
a protein domain that has no
characterized function.
*There are now over 3,000 DUF families
within the Pfam database representing
over 20% of known families

12. REFERENCES
• https://en.wikipedia.org/wiki/Protein_domain