ELISA (Enzyme-Linked Immunosorbent Assay) is a common laboratory technique used to detect the presence and quantify concentrations of antigens, antibodies, and other proteins in biological samples. It relies on the principle of antigen-antibody binding and uses an enzyme-linked secondary antibody for detection. There are different types of ELISA depending on the detection method and procedure, including direct, indirect, and sandwich ELISA. ELISA has many applications, such as disease diagnosis, food allergen testing, and measuring antibody levels after infection or vaccination.