Basic Microscopy

1. Basics of Microscopy
Dr Mathew Joseph MBBS, MD(AIIMS), BCC(Palliative Medicine)
Assistant Professor
Department of Anatomy
Al Azhar Medical College & Super Speciality Hospital
Thodupuzha

2. Learning Objectives
1. How to obtain a single Field of View.
2. Color coding.
3. Movements.

3. What do we do with a microscope?
What does a microscope do?

4. Compound Light Microscope
“Biological Microscope
Magnus”

8. Field of View
• Field of view is the area (circle) that you see
when looking through the eyepiece

9. Calculation of Field of View
__Field Number__
Total Magnification

10. What’s the power of magnification?
To calculate the power of magnification, multiply the power of the
ocular lens by the power of the objective.

11. Magnification
Objective Eyepiece Total Magnification
Very Low Power /
Scanning lens
4X 10X 40X
Low Power 10X 10X 100X
High Power 40X 10X 400X
Oil Immersion 100X 10X 1000X

12. Color Code for Objectives
4X Red 10X Yellow
40X Blue 100X White

13. Appearance of the Specimen and
Movements
• Objects appear upside-down & backward
• Movement appears to be in opposite
direction than actual movement
move slide
slide appears to move
2 1

14. Let’s give it a try ...