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Sam Bradford Research Paper
It seems that this isn't a great day for the Green Bay Packers. You know why? It's because the
former No. 1 pick Sam Bradford has one of his best games right now. Even though that his career
was on a roller coaster ride, Sam didn't give up and proved to them why he was picked #1 overall.
This is the result when the Vikings beat the Packers in a complete upset. He started his first game
with for the Vikings after his offseason was considered "tumultuous". It all began when he signed an
extension with the Philadelphia Eagles, only that he was traded to the Minnesota Vikings
afterwards. It's something that he really didn't expect, especially that he was the former #1 draft
pick. The trade happened 8 days before the start of the season. He admitted that he was struggling to
find his true form, until he has some swagger last night when he outsmarted and outplayed Aaron
Rodgers. As for Aaron Rodgers, the reigning Most Valuable Player, he knew that the Minnesota
Vikings are not to be taken lightly. This is why in every game, he wasn't confident enough ... Show
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It all changed today after the regulation, as the Vikings upset the Packers, 17–14. As a result, they've
moved to 2–0 record. It was believed that two–thirds of the team with this record can make the
playoffs. However, there was a setback when the Vikings won over the Packers. On Jane Slater's
Twitter account, she posted a video of Adrian Peterson, who suffered a nasty injury on his right knee
during the game. He was fine standing up with crutches, on the team's locker room and it wasn't
know when he will come back to play for the team. The Vikings have lost a guy who is really
determined to win. As long Sam Bradford regains his true form with the Minnesota Vikings, expect
that they're going to have a better future ahead. Not only that, they can contend for a playoff run and
a possible championship match with a strong
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Lab Report : Bca Protein Assay
Shrutika Mintri/ E2 Date : 09/17/2014 Lab Report #1 Project Title: BCA Protein Assay Purpose:
BCA (Bicinchoninic acid) Assay otherwise known as the "Smith Assay" has the fundamental
purpose of determining the protein concentration of the two given unknown proteins in the sample
solutions. The absorbance is measured using a Plate reader and a Standard curve is generated. Also,
the different types of pipetting techniques are assessed in this Assay. Methods: (1) There are two
methods using which the BCA Protein Assay can be performed– Test tube method and Micro plate
method. The following experiment has been performed using the Microplate procedure. Vial
Volume of Diluent Volume and Source of BSA Final BSA Concentration A 325 μL 325μL of Stock
1000μg/ml B 325μL 325μL of vial A dilution 500μg/ml C 325μL 325μL of vial B dilution 250μg/ml
D 325μL 325μL of vial C dilution 125μg/ml E 400μL 0 0μg/ml= Blank 1. Preparation of Diluted
Albumin (BSA) Standards: The following Diluted Albumin (BSA) Standards have been prepared
using Bovine Serum Albumin (BSA) Standard Solution (2.0mg/ml) and the Diluent Phosphate
Buffered Saline (PBS) having
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Walmart Is The Nation's Largest Business
The first Walmart was opened on July 2, 1962 in Rogers Arkansas. It was the visionary work of Sam
Walton. In 1967 Waltons family already owned 24 stores and had 12.7 million dollars on sales. By
1980 Walmart had already had 276 stores and employed 21,000. In 1992 Sam Walton said the
company 's mission which was to save people money so they can live better. In 1993 walmart
celebrated its first one billion sales for a week. In 2000 customers were allowed to shop online. By
2002 wal–mart had passed the 500 largest American companies. Walmart has spread to 27 different
countries outside the United States. Now Walmart is the nation 's largest business in the world. It
employs 1.4 million people with 5,239 locations within the United States locations. Walmart has had
questionable ethical business practises in there stores. Low wages at Walmart locations have been a
problem. A report from the National Employment Law Project states that the low prices at Walmart
provided to the customers have a hidden cost to the workers. For Walmart to have it's low prices and
to still have a profit they have to pay low wages to the workers. Its low wages have a negative effect
to all the 2.2 million workers walmart has.This makes it harder for the workers to buy food, housing
and other bills like the water and the light. Wal–mart has plumed wages in it's places of supply by
hiring people outside the company to provide it's services that traditionally are performed within
Wal–mart's warehouse.
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Rhetorical Analysis : Rhetorical Analysis Of Mickey...
Rhetorical Analysis of Mickey through the Years
From Facebook to billboards, our minds are bombarded with advertisements. According to a study
conducted by Red Crow Marketing Inc., the average person sees "10,000 to 40,000" ads a day (Red
Crow Marketing Inc). Among these advertisements are those that sell keepsakes and collectibles.
The Bradford Exchange is a company that sells such astounding keepsakes for the everyday movie
buff. The company has tactfully placed advertisements in magazines such as InTouch, which many
busy mothers and grandmothers buy due to its inexpensive price. Although there are several
Bradford Exchange advertisements throughout InTouch, there is one titled Micky through the Years.
Mickey Mouse, a children's character, opens the market to parents because mothers will buy the
watch to be like their children. From experience, I have seen mothers who become interested in
what their children like to gain respect from the children, hence why the ad is targeted towards
mothers of young children. More importantly, the marketers have skillfully designed the ad to
highlight the "stunning" and "timeless" attributes of the watch (Bradford Exchange Ad). In addition,
the ad also has used color and vivid wording to captivate the minds of those mothers who bought the
cheap magazine for the gossip they do not get due to the busyness of their lives.
Firstly, the mother's eyes will fall upon the watch's display strictly due to the colors that surround
the watch, the
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Extraction and Characterization of Proteins
EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and
principles for protein extraction and characterization were demonstrated in this experiment. Various
proteins were extracted from different sources: 1.67 g yeast invertase, 1.03 g egg white albumin, and
5.15 g of milk casein. Activity assay for invertase was performed using Benedict's test and the
enzymes inverting action on sucrose was confirmed. Warburg–Christian Method and Bradford
Assay were also employed to determine the protein concentration in the albumin and the casein
samples. The concentrations for the albumin and casein samples were found to be 0.519 and 0.327
mg/mL, respectively based on Warburg–Christian Assay; and 6.5x10–3¬ and 1.9x10–2 mg/mL ...
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As a result, the interactions between protein molecules exceed protein–water interactions, and the
solubility decreases. Moreover, the differences in the amino acid sequence makes proteins differ in
their salting in and salting out behavior. This is the basis for the fractional precipitation of proteins
by means of salt. Most commonly used salt is ammonium sulfate because it is available in highly
purified form and because of its high solubility. (Boyer, 2000) Proteins can also be fractionally
precipitated by adjusting the pH, temperature and dielectric constant. Variations in pH also change
the state of ionization of the functional groups, and hence, the net charge of the protein. Usually, the
protein's solubility is at its least at the isoelectric point pI and increases on either side of the pI. At
the pI, the net charge of the protein is zero and the protein molecules do not repel each other. As a
result, protein–protein interactions are increased and the solubility is at its lowest. (Boyer, 2000)
Meanwhile, if temperature is increased denatures the proteins. Proteins then unfold and the non–
polar groups which were previously in the interior of the molecule become exposed. This leads to a
decrease in the solubility of the protein in aqueous environment. Addition of ethanol, methanol,
acetone and the like decreases the dielectric constant and thus decreases protein's solubility. (Boyer,
2000) The
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Unit 4 Equality and Diversity Essay
NCFE Level 2 Certificate in Equality and Diversity
Unit 4: Living in diverse communities
Assessment
You should use this file to complete your Assessment.
The first thing you need to do is save a copy of this document, either onto your computer or a disk
Then work through your Assessment, remembering to save your work regularly
When you've finished, print out a copy to keep for reference
Then, go to www.vision2learn.com and send your completed Assessment to your teacher via your
My Study area – make sure it is clearly marked with your name, the course title and the Unit and
Assessment number.
Name:
1. Describe a range of appropriate investigative methods that can be used to explore diversity in a
community.
Range ... Show more content on Helpwriting.net ...
Through this strategy, barriers to essential services would be unlocked and the potential for social
integration enhanced. Additionally, it was hoped that building capacity in the asylum and refugee
community, would increase opportunities for individuals to grow their skills, knowledge and
competencies as well as their income earning potential.
Olive Branch Trust – Olive Branch Trust was established in 1983 to invest in the local community
of South Bradford, West Yorkshire. Over the years the Trust has grown considerably and now
focuses on 2 main areas of work, namely the Family and the Youth. The Trust employs many people
and these are helped by numerous skilled and dedicated volunteers
In 2001 the Trust also became a Limited Company, and then in 2008/9 Community Interest
Companies were formed to better deliver the services to our clients. There are now 2 companies
namely E:merge CIC (Youth /Education Services) and BD4 CIC (services to the Family). Whereas
the initial focus was our city of Bradford they now provide services to those further afield.
Drug & Alcohol Counselling – Within Bradford there are several organisations offering help with
drug and alcohol problems, organisations like the Council and the Health Authority and specialist
voluntary organisations.
They can give help from practical advice and information to counselling and
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Bradford Assay On Unknown Concentrations Of Proteins
Bradford Assay on Unknown Concentrations of Proteins
Taylor Coleman
September 27, 2016
Lab Group 3
BIOL 1111: General Biology Lab
Fall 2015
Section 107
Chad Perry
Abstract Colorimetric assay is a process of determining a concentration of a solution based on
absorbance of light. The purpose of this lab is to determine if the Bradford assay is an accurate way
to determine an unknown concentration of two samples of protein. The Bradford assay is done by
measuring wavelength of light passing through a cuvette filled with Bradford dye and
concentrations of PBS and proteins. After the cuvettes are mixed they are placed into a
spectrophotometer to measure wavelength. The wavelength given will be used to plot a standard
curve based on concentration (x–axis) and wavelength (y–axis). The standard curve is then used to
measure an educated guess on the concentrations of unknown protein concentrations. We
hypothesized that if we use the Bradford assay and colorimetric spectrophotometry we can
determine an accurate concentration of two unknown concentrations of proteins. The results of this
lab failed to reject our hypothesis based on accurate measurements of protein concentrations. The
standard curves are drawn with a linear increasing slope. The Bradford assay is an accurate way to
demine the concentration of an unknown concentration.
Introduction Colorimetric assay is a process determining the concentration of a chemical element or
compound in a solution
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Wal Mart's Ethical Issues
Although prices at Wal–Mart are considered as reasonable they are still having their own issues in
relation to ethical issues and unethical practices consists of consumers were complaining about how
they were not paying what they consider a fair prices for their groceries. Wal–Mart use to cover up
what they were doing to their customers the advocate group followed the Food Chain Workers
Alliance in order to investigate the root of the problem in the outsourcing ethical systems. Wal–Mart
worker are currently complaining they are not being treated fairly because they are overworking
their employees and they are not receiving enough pay based on their skills and qualifications they
should determine how qualified their employees are for the job of their choice. Wal–Mart just
followed existing law.
Since the central contradiction of corporate social responsibility ethics is reduced to a matter of
noblesse oblige, not human rights. Wal–Mart have been breaking the labor in which it is unethical
practices because the labor department felt they were not being treated properly and the customer
felt they were not giving the right quality of merchandise in which they paid for. According to report
all outlets along with the suppliers are to fully abide to the law regulations in relation to the
immigration labor, safe environment health. Wal–Mart was founded in 1962 in Arkansas by a mean
named Roger's Sam in 2002 Wal–Mart had become a very popular store for consumers to shop
however
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Corporate Social Responsibility : Walmart
Corporate social responsibility has two parts. First, CSR means a company should seriously
consider the impact of its actions on society. Second CSR, requires that a company or individual be
responsible for how their actions impact the society. Walmart is a corporation that some can defend
does display CSR, while other can defend that it does not. Walmart was the epitome of what a retail
chain store should be. Sam Walton took what he learned from the JCPenny model and transformed it
into something better. He was responsible for the social responsibility innovations of the "Buy
American" plan and the "Environmental Awareness" campaign. These innovations display how CSR
was displayed by Walmart. The "Buy American" plan showed corporate responsibility because when
Walton discovered that his company outsourcing was contributing to American job loses he devised
a plan to showcase the domestically purchased merchandise that Walmart was now stocking. This
was an ideal way to incorporate the second definition of CSR into the corporation because Walmart
was considering the impact it had on the society. However, the corporate responsibility Walmart
once tried to incorporate was lost when it became one of the largest purchasers of goods made over–
seas, which in turn forced US manufacturers out of business. In early 2013, Walmart announced
they would be buying American again, however, it is a struggle to find vendors that will meet
Walmart's price points. Walmart could have avoided this
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Essay about What Really Happened During the First...
There can be countless records of the same event, and while many times each record is different,
there are also incidents in which one needs to know every record to piece together what really
happened. The story of the first Thanksgiving takes place in 1621, and is generally known as a meal
that was shared between the Pilgrims and the Indians. For the Pilgrims, this was a harvest festival,
much like one they would have had in England. For the Indians, it was a show of hospitality from
the Pilgrims, similar to what they would expect from visiting another Indian tribe. With being told
only that, one can already see that there are already two very different histories of the same event
recorded. Of greater interest, perhaps, are the different ... Show more content on Helpwriting.net ...
The History Channel's Desperate Crossing tells the story of the Pilgrims, their journey to Plymouth,
their horrible first year, and finally, the first Thanksgiving. Desperate Crossing follows the accounts
of both Winslow and Bradford fairly well, but begins to deviate slightly. They show that the
Pilgrims and Indians played games, drank, and raced, and competed together, some of which are
only vaguely and briefly alluded to by Winslow, and never by Bradford. The History Channel also
shows a much more tense situation than the impression given from Bradford and Winslow.
According to the History Channel, everything from Massasoit showing up with his ninety–some
men and the Pilgrims firing their arms is all a display of strength from both sides.
The History Channel obviously takes some liberties in their presentation of history. Winslow and
Bradford do not mention anything about the clothing worn, if there was any drinking involved, if
there was silverware, where the feasts were held, and many other seemingly trivial details.
Desperate Crossing makes some assumptions on what took place in 1621, but it does quote both
Winslow and Bradford word for word. While we're shown a picture that may or may not actually
represent what took place at the first Thanksgiving, at the very least, we're given Winslow's and
Bradford's descriptions of it. The History Channel used what was already known from the eye–
witness accounts combined with what
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The Case Of Wal Mart
When we hear someone say they need to go to the grocery store, what is the first place that comes to
mind? For most of us, it is Wal Mart. From its market end, to its home and beauty supply, all the
way to its lawn and garden department, Wal Mart has been ordained one of the most profitable
companies in the entire world. But it did not start out that way. With a dream, determination,
leadership, and some sweat, Sam Walton, made a small mom and pop store into the multibillion
dollar company it is today. On March 29, 1918, Samuel Moore Walton was born to Thomas and
Nancy Walton of Kingfisher, Oklahoma. As a young boy Walton and his family moved locations
and found themselves living in the small town of Columbia, Missouri. Growing up, Walton excelled
at school, making the top grades of his class. He was also known for his athletic ability, and in high
school was the quarterback for his high school football team (biography.com). When Walton
graduated high school in 1936, he was voted "most versatile boy" (biography.com). Little did
anyone know that that most versatile boy was going to achieve great success in just a few short
years. After graduating high school, Sam decided to further his education and attended the
University of Missouri. He graduated in 1940 with his degree in economics. Shortly after
graduation, Walton joined the army at the mere age of 24. He served until 1945 as Captain of an
intelligence unit (biography.com). Upon returning home
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The Spectrophotometer Determination of Protein...
The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl
Sulphate And Triton X–100 Have On Protein Concentration.
INTRODUCTION
Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of
light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the
absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions
(Tombs, et.al, 1959). Spectroscopy is the use of electromagnetic radiation by a sample in order to
identify components of the sample (qualitative analysis) or to measure the amount of material in that
sample (quantitative analysis) (Tombs, et.al, 1959). Absorption is a process in which ... Show more
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MATERIALS AND METHODS
The BIORAD dye was firstly prepared by dissolving 0.225g of Coomassie Brilliant Blue G–250
(Merck, South Africa), in 67.5ml of Methanol (Riedel–de–Haёn, Germany) and 291ml of 85% (w/v)
Phosphoric Acid, diluted to 450ml with dH2O. Six test tubes were labelled SC1–6 and the 0.1mg/ml
stock of Bovine Serum Albumin (Roche Diagnostics, Germany) and dH2O was pipette into their
respective test tubes according to Table 1.
Table 1: Table of the BSA and dH2O volumes to be pipette and placed in the spectrophotometer to
obtain absorbencies for the standard curve
Test Tube Number Total Volume (μl) Protein (BSA) Stock (μl) H2O (μl) Amount of Protein (μg)
1 2400 0 2400 0
2 2400 50 2350 5
3 2400 100 2300 10
4 2400 150 2250 15
5 2400 200 2200 20
6 2400 250 2150 25
600μl of the BIORAD dye agent was pipette into each test tube making up the volume to 3000μl.
All the test tubes were then covered with Parafilm and placed in the Vortex (Lab Pro cc) for a few
seconds and they were incubated at room temperature for 5–60 minutes. Simultaneously another set
of test tubes were labelled X1–8 and pipette with 0.1mg/ml Lysozyme stock (Merck, Germany),
BSA, 1% (w/v) Sodium Dodecyl Sulphate prepared with dH2O, 1% (v/v)
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The Organization's Stand, Diversity Audit
Conduct Audit
In order to perceive about the organization's stand, diversity audit is vital and the separation that is
needed to cover its objectives area. Notwithstanding this, implementation and planning diversity
audit is a crucial procedure and there are some key focuses that Sam 's West, Inc. (working together
as Sam 's Club) ought to consider with a specific end goal to direct differing qualities review in a
compelling way (Oberoi, 2014). These are portrayed as beneath:
The audit must cover every one of the gatherings of workers and HR procedures of the firm on
account of diversity impacting individuals of the firm. Besides, it ought to concentrate on both
quantitative and qualitative information.
Sam 's club ought to hope to characterize the principle reason for auditing. For case, there might be
various motivations behind auditing like to deviation from the general procedure, to gauge
representative input, survey business effect of assorted qualities mediation, future strategy, and so
on. Besides, Sam 's club ought to likewise confirm that how every now and again it needs to direct
the review.
Primary and secondary research likewise helpful so as to direct audit in a viable way.
Primary and Secondary Research
Primary Research
Primary research is an examination or the gathering of information firsthand, or by an outsider
contracted particularly for the firsthand party. Research you do yourself that has not been done some
time recently (Kleynhans, 2013).
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LDH Purification lab Report
PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE
Abstract
The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is
important for the normal function of the body. Purification of LDH is essential to understand its
structure and function. The purpose of this experiment was to extract and purify LDH enzyme from
chicken muscle tissue using a variety of various. Analytical methods such as activity and protein
assay were employed to determine the presence and purity of LDH. The cells were initially
disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated
protein mixture by affinity chromatography and its activity was studied by ... Show more content on
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10 microliters of the sample is then added and the assay absorption is measured at 340nm. If
absorbance was above 1.5, samples were diluted. Protein Assay: The Pierce BCA Protein Assay
(Thermo Scientific) is a detergent–compatible formulation based on bicinchoninic acid (BCA) for
the colorimetric detection and quantitation of total protein concentration. A series of standard
solution of Bovine Serum Albumin (BSA) ranging from 0–2000 µg/ml was prepared from a stock
solution of 2 mg/ml BSA. 25ul of diluted crude (1:500, 1:250), desalted (1:100, 1:50), and 6 peak
fractions from cibarcon blue column (1:10, 1:5) were loaded in microplate along with 175ul of BCA
working reagent. Microplate was incubated for 30min at 370C and then the absorbance was
measured at 562nm.
Results/Discussion The purpose of this experiment was to extract and purify LDH enzyme from
chicken muscle tissue using a variety of techniques including homogenization, ammonium sulfate
precipitation, dialysis, and affinity chromatography. Activity and Protein assay were used to track
the overall amount of LDH present in the samples. Crude Extraction: Chicken muscle tissue was
homogenized in a blender with cold extraction buffer in order to lyse cells, releasing LDH into
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Protein Concentration Lab Report
Figure 3. Bradford Standard Curve using 10mg/ml BSA Protein. 10mg/ml BSA protein stock was
serially diluted to cover a 5–2000µg/mL concentration range. 50 µL of dilutions were added to
1.5mL Bradford Reagent, left at room temperature for 5minutes, and assayed at 595nm, against a
blank of 50 µL DI H20 and 1.5mL Bradford Reagent.1
________________________________________ From the Bradford standard curve equation, the
protein concentration of the crude lysate was calculated to be 0.1402 µg/mL, and the protein
concentration in Fractions 1, 2 and 3 was found to be 11.5038 µg/mL, 11.5038 µg/mL, and 26.6553
µg/mL, respectively. Although Fraction 2 appeared to have the most intense yellow coloring,
Fraction 3 had the highest protein concentration. ... Show more content on Helpwriting.net ...
The Stokes radius of a molecule is the effective radius a molecule has as it moves through a
solution. Molecular weight of a molecule is sometimes correlated with the Stokes radius of a
molecule, and thus the elution trend in gel–filtration, but this is not without exceptions. Thus, Stokes
radius is a better predictor, because although two molecules may have the same molecular weight, a
longer, more branched molecule will have a larger Stokes radius than a smaller molecule.1 In the
separation of blue dextran, cytochrome c, and potassium chromate, the molecular weight of the
molecules were proportionate to their molecular sizes. The largest molecule, blue dextran, eluted
first, followed by cytochrome c, and then the smallest molecule potassium chromate. Blue dextran
has the largest molecular weight, and potassium chromate has the smallest. The results for elution
order are in accordance with what the theoretical, predicted results were. The efficiency of the
separation was not as ideal as would be predicted on the basis of molecular weights. The molecular
weights of the molecules are 3 orders of magnitude different, and should produce well–resolved
peaks.1 Experimental visual results show peak overlapping around 2mL and 3mL, as the colors
were mixed at these elution volumes. This peak overlapping may be attributed to peak broadening.
Peak broadening has many causes, but of particular
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Causes Of Civil Disobedience
Although civil disobedience rarely accomplishes the change that it demands directly, it does create
change indirectly through raising awareness and support. Civil disobedience works by relying on the
reaction of governments/societies after individuals rebel to rally support for the protest. If the
government/society violently crushes the rebellion, as in the case of Antigone, then the civil
disobedience wins by creating pity and sorrow for the rebellion. However, if the government/society
does not react, then the individuals are still successful by being allowed to get away with acts of
civil disobedience which spread their cause.
Despite the tragedies surrounding the American Civil Rights Movement, change could not have
been made without the violent reaction to the movement. Rather than stopping or slowing
desegregation as the violent reactions intended, they only created martyrs for which the Civil Rights
Movement could rally around. Famously, J. W. Milam and Roy Bryant kidnapped and murdered
Emmett Till, a fourteen–year–old African boy, on account of flirting with a white woman. Then, the
guilty party was found innocent despite overwhelming evidence otherwise. Although this incident
may have been notable on its own, it became a very powerful motivation for the Civil Rights
Movement through the confession/arrogance of the kidnappers, and the work of Emmett Till's
mother, Mamie Till. Infamously, Roy Bryant and J. W. Milam sold the story of Emmett Till to
reporter William
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Absorbance Values
Abstract:
Claims were made that manufacturers had improperly labeled protein concentrations on their
products. Concentrations of these three different protein–containing liquids, whole milk, soy milk,
and a protein solution were determined using a Bradford dye reagent and a spectrophotometer to
measure the absorbance of each. Absorbance of a set of standard known protein concentrations from
a range of 0.125 mg/ml to 2.000 mg/ml were measured using the spectrophotometer at a set
wavelength and this data was plotted. The equation generated from the best fit line of this data was
used to determine the unknown concentrations of the three different samples. These values were
then compared to the protein concentrations obtained from the corresponding ... Show more content
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This would indicate that either the food labels or the calculated data are incorrect. The data was
dependent on a linear relationship between absorbance and protein concentration. However, the
linear relationship was based on only seven data points, two of which were omitted due to
inconsistency, and was only representative up to 2.000 mg/ml protein concentration. Because the
data sample was small and covered a small range of concentrations, the food label concentrations
cannot be determined as inaccurate. The more likely cause would be that the linearity assumption of
the correlation between the absorbance and the protein concentration is
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Phl/458 Famous Thinkers Essay
PHL/458
October 8, 2012
Famous Thinkers
While thinking of computer software, you begin to think of Microsoft. In fact if you use a computer,
chances are that you will have some type of program on there that is developed by Microsoft. Bill
Gates is the chairman and co–founder of Microsoft Corporation, the worldwide leader in software,
services and solutions that help people and businesses realize their full potential. When it comes to
discounted variety stores there are not many stores that compare to Wal–Mart. The founder and
former chairman of Wal–Mart Stores Inc., the world's largest retailer, is Sam Walton. The innovative
thinking of Bill Gates and Sam Walton has changed the business world. With Bill Gates'
contributions to ... Show more content on Helpwriting.net ...
Guided by a belief that the computer would be a valuable tool on every office desktop and in every
home, they began developing software for personal computers. Gates' foresight and his vision for
personal computing have been central to the success of Microsoft and the software industry. Under
Gates' leadership, Microsoft's mission has been to continually advance and improve software
technology, and to make it easier, more cost–effective and more enjoyable for people to use
computers. The company is committed to a long–term view, reflected in its industry–leading
investment in research and development each year (Microsoft, 2011).
Samuel Moore Walton was born March 29, 1918 in Kingfisher, Oklahoma. His dad, Thomas Gibson
Walton, was a banker, farmer, farm loan appraiser, and real estate and insurance agent. Sam Walton
had an entrepreneurial spirit from an early age and after graduating from the University of Missouri
at Columbia in 1940, he began his lifelong career in retail as a management trainee at J.C. Penney.
He joined the military in 1942 where he served until 1945 when he was released. While in the
military, Sam Walton's wife decided that she wanted small town living so they moved to Newport,
Arkansas where Sam Walton purchased a Ben Franklin five–and–dime franchise. By the early
1960's Walton owned 16 Ben Franklin stores in Kansas, Missouri, and Arkansas but he was not
seeing the profits that
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Material And Methods : Bm Mscs Cultivation
Material and Methods: BM–MSCs cultivation: BM–MSCs were obtained from Institute Pasture of
Iran and were cultured in Low Glucose Dulbecco 's modified Eagle 's medium (Low Glc–DMEM)
(Gibco Invitrogen, Karlsruhe, Germany) with 10% FBS (Gibco Invitrogen, Karlsruhe, Germany)
and 1% penicillin and streptomycin (Sigma–Aldrich, Schnelldorf, Germany). Then, BM–MSCs
were incubated in humified incubator at 37˚C and 5% CO2. Cell's medium was replaced every two
days. U266 cell line: Confirmed U266 cells, a human myeloma cell line, were purchased from
Institute Pasture of Iran. U266 cells were grown in RPMI–1640 (Gibco Invitrogen, Karlsruhe,
Germany) with 10% FBS (Gibco Invitrogen, Karlsruhe, Germany) and 1% penicillin and
streptomycin (Sigma–Aldrich, Schnelldorf, Germany) in T25 flask culture (Orange Scientific,
Braine–l 'Alleud, Belgium). Then, U266 cells incubated in humified incubator at 37˚C and 5% CO2.
The medium was changed every day during all steps of experiments and Trypan blue staining was
used for viability analyzing. BM–MSCs derived C.M preparation: The BM–MSCs were cultured in
T25 flask culture (Orange Scientific, Braine–l 'Alleud, Belgium) with DMEM medium and
incubated for reaching to 60–70% confluency. In the next step, DMEM medium was replaced with
RPMI–1640 medium without FBS and was incubated for 72 hours (hrs). After incubation; medium
was collected, cells and debris were removed by centrifugation. Supernatant was concentrated by 10
kDa MW cut–off ultrafiltration
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Globalization of Walmart
Globalization of Walmart
Mg 460
George Rideout
Lisa Baron
November 10, 2014
The location of the first Wal–Mart in the Fortune Global 500 for the year of 2001 to 2002 turnover
of 219.81 billion dollars. Wal–Mart is the largest company in the retail in the world. The company
was much larger than its competitors in the United States – Sears Roebuck, Kmart, JC Penney and
Nordstrom combined. In 2002, Wal–Mart operates more than 3,500 discount stores, Supercenters
and Sam's Clubs in United States and over 1,170 stores in major countries around the world. The
company also sells products online via the website, www.walmart.com. Wal–Mart is one of the
largest private employers in the world, with the use of force about 1.28 million. The ... Show more
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Wal–Mart failed to grasp the consumer and retail environment in Japan. With a population of 127
million, the highest per capita income and the second largest economy in the world, Japan is a very
smart market for retailers. The opportunity exists, but there is much more research and planning that
needed to be done before expansion began. Instead of adapting business operations to the Japanese
culture, the company essentially assumed the Japanese would readily adjust to Wal–Mart's. For
example, in Japan there is a much larger need for local store customization. Consumer buyer
behavior is much different than in the United States, with purchasing patterns and product selection
varying greatly between regions. They have a trend to buy smaller quantities in regular intervals
rather than the more American idea of "stocking up." Similarly, the concept of large retail stores is
foreign. Retailers with the highest growth rate are small specialty stores; quite the opposite of Wal–
Mart. The culture tends to buy more fresh produce than pre–packaged goods as well. Lastly, the
Japanese view high price as equaling high quality. This mentality causes them to purchase forty
percent of the world's luxury goods annually. Packaging and appearance of goods play a huge role
in their purchasing decisions. When looking at Wal–Mart's product selection, it is obvious they do
not usually cater to luxury–brand customers. All of these cultural misunderstandings lead Wal–Mart
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The Importance Of A Good Business Communication Network...
Analysis of Wal–Mart Company Patricia Diggs INF220: IS Principles (CLH1448A) Instructor:
Torre Wilson December 15, 2014 Computer technologies and inventions have revolutionized the
way business is done in our current age. I believe it would be safe to say that no business can
survive today without computers, primarily for their fast communication channels available. A good
business should have a good communication network infrastructure set up internally for fast
communication between the workers and externally to suppliers, and their market. So what are the
characteristics of a good business communication network design? Before I get into that I would
like to choose a company that I think has a good business communication ... Show more content on
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Wal–Mart has employed over 2.2 million associates across the globe and about 1.3 million in the
USA (Wal–Mart company website, 2014) Maintaining communication in such a huge work force is
a daunting task. Wal–Mart communication network reaches all its employees from the cashier to the
CEO. The company's progressive innovation in their technology has been the key to their global
success. Wal–Mart's technological investments have kept the company's products at low prices and
available and accessible to its customers. Wal mart has outsourced their technical communication so
that they can concentrate more on the business itself. They have also outsourced their database,
which is the world's largest commercial database at 7.5 terabyte, to NCR Corporation. They also use
software that helps the management to more effectively communicate with other departments for
instance the accounts and human resources departments. Neo Vista Company who tailored the
software for the requirements of the NCR database environment provided for this software. This
type of access to data is known as data mining where any employee of Wal–Mart could access the
database and get key information about any item sold or is available for sale at any time the
information is required. This has proved
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Bus 110 : Fall 2015
BUS 110 – Fall 2015 Final Project, due 12/9 This assignment allows you the opportunity to examine
a publicly traded company. Required: 1. What is the name and mission statement of the company? a.
The company's name is Walmart and their mission statement of the company is ( " We save people
money so they can live better") 2. Describe the company's main product(s) or services. Where do
they sell their product? Are they an international company? Where are their stores? a. The
company's main products are clothes, toys, sporting goods, and groceries. They sell their product to
the United States. They are an international company. They have stores in Alaska, Japan, Canada,
United Kingdom, China, India, Nigeria, United States, Mexico, Ghana, Brazil, Uganda, Kenya,
Tanzania, Mozambique, Zambia, South Africa, Namibia, Botswana, Brazil, Argentina, and Chile. 3.
A history of the company telling when it was founded, where and by whom. In most companies you
will find an interesting story on the origins of the company. Summarize major events and tell how
those events affected the company. A timeline may be a great way to show major events. Walmart
was founded in 1962 by Sam Walton in Rogers, Arkansas. Some major events in this company are
Timeline 1950 On May 9, 1950 Sam Walton opened up Walton's 5&10 in Bentonville, Arkansas. In
1962 Sam Walton opened the first Wal*Mart in Rogers, Arkansas In the past fifty years, Walmart
has grown rapidly, they have 2 million people
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Walmart
Wal–Mart study Case#1 1. What threats and challenges is Wal–Mart currently facing? From the
beginning, Walmart did not have many threats. However, not only the competition is different,
several global retailers such as Target, Carrefour, Costco, and Amazon, are working hard to keep
efficiency. They are trying to work together to shrink the prices difference between them. Walmart
has facing difficulties from every single angle. Not only the company has internal labor relation
problems, but also it has some external threats from its competitors. The company must work hard
to get possible solutions against its competitors, and to solve any internal problems regarding its
labor relations. Even though Walmart does not have any problems ... Show more content on
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However, in 2008, there was a change from $8.63 to 10.83 per hour. Managers at Walmart do not
respect employee's rights, and they sometimes treat employees in a way that is against the law in the
United States. Managers even sometimes force employees to work off the clock and skip lunch, and
it is again against the law. The CEO needs to pay attention of such a situation. Because of the labor–
related issues, there were several lawsuits against the company in 2008 in the following States:
Colorado, Minnesota, Oregon, etc. There was also a gender discrimination situation where Women
claimed in 2007 they were not treated fairly by the company. Even though people consider Walmart
the company that employs more than 1.4 employees, it does not pay good salary to its employees. It
is against the law to treat people in such a way. In 2002, Walmart used a type of competition that
was not normal 3. Wal–Mart's Board discussed proposals to meet (some of) these challenges at a
board retreat in 2005. Discuss these initiatives as outlined in the "Supplemental Benefits
Documentation: Board of Directors Retreat FY06" See also Wal–Mart health cost strategy NYT
26Oct2005. Was additional damage done to Wal–Mart by the leaking to the public of the Board
Benefits Strategy document? Challenges are considered the biggest issues for the Wal–Mart
company. Even though there advantages, disadvantages
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Walmart Is A Multinational Retail Company
Walmart Organization Walmart is a multinational retail company that runs a network of more than
11,000 discount retail stores as well as warehouse stores distributed in more than 27 countries. Its
headquarters are located in Bentonville, United. It ranks as the world's leading private employer
with over 2.2 million employees serving in different countries' retail stores. Walmart operates as a
family owned organization that is managed by the Walton family. The Walton siblings own more
than 50% of this company through managing the Walton Enterprises as well as through individual
holdings (Charles, 2014). Walmart is equally ranked among the highly valuable companies, in terms
of market value, as well as the biggest grocery retailer where it generates more than 51% of its sales
from the grocery business. This paper explores Walmart's operation management with regard to
supply chain characteristics, global business operations, production processes, the company
commitment to quality and excellence, inventory methodologies, operational planning and
movement towards lean processes (Massengill, 2013). Walmart Organization History and
background information Walmart's history dates back in 1945 when Sam Walton bought an outlet of
the Ben Franklin retail stores with the aim of selling products at a relatively low cost, so as to
generate huge sale volumes and make profits at a low margin. Although this portrayed the purchased
outlet as a crusade for most consumers,
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Walmart Mart 's Impact On The United States
In 1950, Sam Walton obtained a little store from Luther E. Harrison in Bentonville, Arkansas called
Walton 's 5 & 10. Walton 's 5 & 10 is currently called WalMart Visitor 's Center in Bentonville,
Arkansas. In 1962, author Sam Walton opened the first WalMart store situated in Rogers, Arkansas.
Sam Walton was propelled by other rebate office chain stores. By keeping his retail costs lower than
alternate contenders stores he found himself able to open his second WalMart store that year in
Rogers, Arkansas. There were an aggregate of 24 stores in the condition of Arkansas by 1967 with
profit of $12.6 million in deals.
In 1968, the first store was opened outside of Arkansas in Claremore, Oklahoma and Sikestone,
Missouri. WalMart extended ... Show more content on Helpwriting.net ...
"Because of this staunch hard working attitude and commitment to client care, WalMart asserted
from the get–go that a formal morals system was pointless in light of the fact that the organization
had Mr. Sam 's morals to take after" (Ferrell, Fraederich & Ferrell, p. 408).
WalMart is known not the biggest retailer on the planet additionally known for low advantages and
wages. This is a moral issue that was tended to. It was said that WalMart needed to procure healthier
and low maintenance specialists. This was terrible press for WalMart and they needed to do
something to enhance their association with worker stakeholder 's.
Making the moves to enhance medical advantages and wages is the thing that the organization chose
to do. In spite of the fact that WalMart improved upgrades by actualizing social insurance and better
wages despite everything they missed the mark when they chose to diminish their workforce. This
was bad on the grounds that client 's are expecting extraordinary client administration and with
constrained representatives it is difficult to give great client administration.
Another moral issue that WalMart has had press on was "Who 's Responsible for the WalMart
Mexico Scandal?". This story hit the New York Times in April 2012, expressing that WalMart was
included in a pay off in a Mexican auxiliary. It was purportedly said that WalMart
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Sustainability Initiatives A Demonstration Of Corporate...
What are the cost benefits for corporations leading sustainable efforts in their communities and
should corporations be financially responsible for the environmental disturbances that they cause, be
it directly or inadvertently? More specifically are the efforts of Wal–Mart to implement
sustainability initiatives a demonstration of corporate social responsibility or just greenwashing?
Relevant to these questions we hypothesis that despite the multitude of efforts that are resulting in
some groundbreaking policies for an Oligopoly such as this, that the fact that they still source,
manufacture and distribute products in a manner that is unsustainable which makes these collective
efforts an example of "social" greenwashing. Investopedia ... Show more content on Helpwriting.net
...
Is this truly a serious initiative for Walmart to use their size for good or is this just greenwashing?
Since their CSR plan announcement Walmart has been making progress towards achieving those
goals but many don't think it's enough or realistically sustainable for the long run. Walmart has
received backlash for their slow adoption of renewable energy, it's cheap products, and it's little
progress on their sustainability index (Gunther, M., 2011). One of Wal–mart 's initiatives intended to
address some of their inimical externalities is to convert to 100% renewable energy to power their
stores. An article published in 2011 stated that Walmart was powered by only 2% of renewable
energy and the ILSR came out with a report stating less than 2% of renewable energy powers
Walmart stores. People were not impressed with this minimal progress and pointed out that other
companies like Starbucks and Kohls use more renewable energy than Walmart. It was also pointed
out that Wal–mart is a huge multinational corporation and that a process like this will take time,
especially if they 're trying to avoid raising their costs, which in turn would not benefit the
customers. However, in 2013 it was reported that Wal Mart 's 10,800 stores are powered by 21% of
renewable energy, which is a decent amount of growth from
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Bradford And Lowry Assay Lab Report
The Bradford and Lowry assay are two color changing proteins with similar processes. Color
changes of the solution can be measured using a device called a spectrometer. In lab, the two assays
can be used to determine the amount of protein in a solution. This can be done in an experiment by
reacting a specific reagent with amino acids or peptide bonds present in the proteins. This produces
a color change in the solution depending on the amount of peptide or amino acid reactions with the
reagents. This color change can then be measured using a device called a spectrophotometer.
The Bradford assay is a one– step process, which involves the Coomassie dye reacting with the
amino acids arginine and lysine. It under goes changings in acidic conditions ... Show more content
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The type of amino acid sample affected the ability of the reagents to react with the peptide bonds
and amino acids. It also affected the amount of amino acids and peptide bonds in the protein. Each
of the four proteins used in the experiment had the same concentration, which was reflected in the
accuracy of the standards. In relation to the Bradford reagent, the amount of reactions that occurred
depended on the amount of arginine and lysine amino acids present in the protein. In relation to the
Lowry reagent, the amount of reactions that occurred depended on the amount of tryptophan and
tyrosine amino acids in the protein and the number of peptide bonds linking the amino acids. The
absorbance measured in the spectrophotometer was affected also by the differences in amino acid
compositions and the ability of the reagents to react as a result. Since all the protein standards had
the same concentration of 0.5 mg/mL, it allowed for the accurate comparison of the absorbance,
mean, and standard deviation of each of the
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Case Study Walmart
Allison Whitted Kaplan University Case study paper Wal–Mart in China GB520: Human Resources
Management Introduction In this case study I will be discussing the company Wal–Mart Stores:
Every Day low Prices in China. By the time 2005 rolled in China has become the most prominent
country to set up a branch for stores. China had a huge open market and it was time for the company
to take advantage of the opportunity that was happening in China. Wal–Mart has become the world's
largest retail chain. This can only mean one thing and that is expansion. The strategic move to
expand the company chain stores to China was based off of the following factors. First factor was
China size as far as land, population. They also had a booming middle ... Show more content on
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According to the article experts says that Wal–Mart customers saved at least 15% on a typical cart
of groceries (Farhoomand, A. F., Wang, I., September, 2008). The major issue for Wal–Mart was
how to salvage the investment they made in China. They had these entire great strategic plan that
worked in other countries that seemed to have done great. By 1996 the company decided to move a
branch into China they started with a supercenter and a Sam's club. The business did not take off the
way they hoped and planned. The company started to lose money since it opened stores in China.
The company thought that the problem was that they had only one store in such a huge market. The
fact of the matter was that the Chinese government turned down the company request to expand
further in different parts of the country. The government was not convinced that Wal–Mart would be
successful with all the new competition that was being implemented in other areas. Conclusion
Wal–Mart learned that businesses in other countries may impact the benefit of an investment and is
also learning that it's going to be trail and error. They can only hope that they can endure their place
there so that they can have the potential to grow as a brand. I recommend that the company continue
to research and discover what is in those local stores that are driving their consumers to keep
coming back for more. References Farhoomand, A. F.,
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Walmart Management
Management Paper on Walmart
Wal–Mart Stores: Managing Diverse Organizations
By
Angela L. Farrish
Webster University
Ms. Amy Thenor
December 2011
Wal–Mart In 1970, Sam Walton the founder of Wal–Mart implemented an extremely significant
warehouse distribution system. This new system evolved into Wal–Mart Stores Inc. and in that same
year the company was publicly traded on the New York Stock Exchange. The company began to
expand extremely rapidly throughout the 70's. During this period Wal–Mart started incorporating
pharmacies and auto service centers into its stores which incited even more growth. By 1979, there
were 276 Wal–Mart stores in 11 states. During the early 1980's Wal–Mart's ... Show more content on
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This motivational strategy allows employees to benefit from their performance which will in turn
make the company's overall performance better. Home office management associates are eligible to
receive additional compensation through stock options. Associates can become shareholders through
the Associate Stock Ownership Plan. Wal–Mart also contributes 15% each year toward associate
stock purchases. This contribution is capped at $1,800 each year. Since the typical Wal–Mart
employee only makes between $6 and $7.50 per hour, most are unable to afford the stock options
and only one in fifty associates participate in the program. Wal–Mart associates also receive 10% off
selected store merchandise and are offered discounted child care through four national providers
(Peterson Coleman). These benefits help Wal–Mart keep its best and brightest employees.
Diversity Challenges
Problems in businesses conducted across cultures often arise when individuals from one culture are
not able to understand culturally determined differences. The most common difference is the
language barrier if individuals are not fluent in the language. With individuals, who don't speak the
same language, the use of nonverbal cues can explain what is trying to be communicated. Nonverbal
cues and behaviors vary from one culture to another. For example, people from the U.S. are taught
to use direct eye contact when communicating; in many
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The shape and magnitude of the UV spectra depends on the...
The shape and magnitude of the UV spectra depends on the composition of amino acid in each
protein. Due to the aromatic amino acid residues in the protein, the observed UV absorbance was
mainly in the 240 nm to 340 nm region. In Figure 1 to 3, the maximal absorbance of each protein
was approximately at 280 nm. The difference in magnitudes of the peak observed was linked to the
differences in the amino acid contents in each of the proteins. The peak of lysozyme was greater
than those of BSA and gelatin, because lysozyme has a greater number of tryptophan residues.
Lysozyme has six tryptophan residues, whereas BSA and gelatin has two and zero, respectively
(Department of Chemistry, 2014). Lysozyme has three times more tryptophan residues ... Show
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In order to examine the variation in sensitivity of lysozyme, gelatin, and BSA, the absorbance
reading was obtained using the Bradford and BCA assay
Gelatin is observed to have a lower absorbance reading than lysozyme in only the Bradford assay,
while in the BCA assay gelatin was observed to have about the same absorbance as lysozyme. In
Bradford assay, the color yield for proteins with higher content of tryptophan, tyrosine, or cysteine
residue, will be higher. The observed protein sensitivities did not correlates with the amino acid
content in the Bradford assay. As presented in Table I, the mole fractions of lysine and arginine in
BSA were 10 mole percent and 4 mole percent, respectively. The sensitivity should have been
higher in BSA than in the Bradford assy. However, the observed sensitivities did correlates with the
amino acid contents in the BCA assay. Bradford assay would be ideal if an accurate estimation of a
newly isolated protein using BSA as a standard. With the data obtain from the Bradford assay, it
shows that it was more sensitive and quicker, also it had less interference from substance that were
possibly present in the protein solution, expect for the interfering substance SDS.
The interference of RNA and ME in the Bradford assay was compensated by using the blank,
because the difference between the addition of the protein and the non–protein blank
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Football : The Causes Of A Football Fire In Football
One of the main reasons why I got interested in this event was that the fire took place during a
soccer game. Soccer is by far my favorite sport to play and to watch. So when I heard about this
tragedy, it got my attention because I have always been a big soccer fan. It's hard to understand that
fans came to watch an exciting soccer game, but then 56 people ended up dead. This is why I was
intrigued by this event and how this could possibly have happened.
Valley Parade stadium in Bradford City, West Yorkshire, England goes down as the worst fire
disaster in football (soccer) history. On May 11, 1985, what started out as a wonderful celebration
ended in 56 deaths and 265 injuries. So many people died that day and it should not have happened.
There were so many warning signs that were ignored by the owners which resulted in 56 deaths
ranging in ages from 11 to 86. The stadium design, and the materials used as well as the lack of
planning all contributed to this disaster. This fire changed how football stadiums are designed to
ensure the safety of the fans.
On Saturday afternoon, 11,076 fans arrived at the Valley Parade stadium to watch Bradford City
versus Lincoln City. Before the start of the game, Bradford City was presented the championship
trophy in their final Division Three game. This was the team's first Division trophy in 56 years. It
was an exciting time at the game and for the city. A little bit before half–time, with the score 0–0,
some smoke could be seen coming
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A Diagnostic Tool For Renal Disease And Hypertensive...
Introduction Proteinuria is often used to detect the presence of any type of proteins in the urine and
serves as a diagnostic tool for renal disease and hypertensive disorders. Among those disorders, pre–
eclampsia is characterized by low renal perfusion and leakage of proteins in urine due to a damaged
glomerular base membrane (1). This condition is associated with a high risk of maternal
complications and prenatal mortality. Pre–eclampsia is usually defined as a combination of
hypertension, edema and proteinuria after 20 weeks of gestation and is determined by a threshold of
300mg/24 hours of proteins (2). Normal urine excretion is up to 150mg/day (3). The most common
test used in antenatal care to screen proteinuria is the visual reagent dipstick. It is a semi–
quantitative and colorimetric method. The stick changes colour when the reagent
(tetrabromophenol) binds to the proteins. The binding is pH dependent (albumin binds at a pH
between 5 and 7, other proteins bind only at a pH below). If the urinalysis by the dipstick is positive,
a 24 hour–urine collection is performed in order to confirm the proteinuria and to estimate the total
amount of protein (4). Another method available for detecting proteinuria is called the Bradford
assay. This quantitative method is based on the binding of proteins with the dye Coomassie Blue
which increases the absorption of the dye at 595nm. The absorbance measured by
spectrophotometry is related to the amount of proteins present in the
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Marketing Environment Analysis Of Wm Morrison Supermarkets...
Introduction
This assignment, as part of the Marketing module examination, will first include a marketing
environment analysis of Wm Morrison Supermarkets PLC for both the internal and external
environment of the company. Secondly, a SWOT (Strengths, Weaknesses, Opportunities and
Threats) analysis will be conducted followed by strategic recommendations concerning the
company's future operation. Prior to the above–mentioned contents of this assignment is a brief
description of Wm Morrison Supermarkets PLC's profile. Last but not least, a paragraph at the end
of this assignment will conclude all of its contents.
Morrisons Brief Description
Morrisons is a chain of supermarkets, currently being the 4th largest in the UK with a market share
of 11% (Butler, 2014). It is a Public Limited Company whose legal name is Wm Morrison
Supermarkets plc. The company's headquarters are located in Bradford, England (Morrisons,
2014a). Morrisons had 127,403 employees as at 02/02/2014 (Keynote, 2014), more than 500 stores
(Morrisons, 2014d) and over 100 convenience stores (Morrisons, 2014b). Τhe company's main
strategy is to capture growth and market share, increase its efficiency and finally remain and maybe
improve its position in the top 4 supermarkets' list (along with Tesco, Sainsbury's and Asda)
(Businessweek, 2014). In addition, Morrisons tries to buy local products when possible and in many
cases manufactures products at its own sites (Morrisons, 2014c).
Marketing
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Health And Safety At Work Act
Health and safety at work act The health and safety at work act is a law which was put in place in
1974, this is to make sure that the people at work are looked after in the correct way and make sure
that the work environment is safe. Also to ensure that they don't work in dangerous conditions and
they use the right clothing and safety equipment. They have to make sure that they check the
materials used and that they are stored and handled properly. They have to have a written safety/risk
assessment form for the workplace. This is for everyone within the workplace including employers,
suppliers, self–employed, trainees and manufactures. The health and safety at work act also ensure
that the health and safety of others, for example the ... Show more content on Helpwriting.net ...
This may cover an incident in which the work equipment, environment and activities were carried
out, organised or supervised, lead in some way to the accident that happened. The reports which is
filled out will alert the enforcing authorities to the incident which happened, depending on how
serious the circumstances are the enforcing authorities will decide if it is suitable to investigate.
Sporting example: When using the gym equipment they have to carry out an induction so that
everyone is using the gym equipment safely and correctly. If they don't use it properly they could
easily hurt themselves or other people around them. First aid at work act The health and safety (first
aid) act ensures employers supply appropriate equipment, facilities and personnel for example a first
aid box which includes plasters, bandages, medical tape etc, and staff are first aid trained. It depends
on how serious the incident is in the workplace, this will determine on the how much first aid is
needed. These regulations apply to all workplaces, these also include those with less than 5
employees and the self–employed. Sporting example: When Fabrice Muamba collapsed on the field
during a football match, there were people that were first aid trained, so they could treat him and get
him the help he needed quickly. His circumstances could have been a lot worse if there wasn't
anyone
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William Bradford Research Paper
"'Let's hurry back to the 'Mayflower' and bring our families ashore, William declared. But when they
reached the 'Mayflower,' one person was missing. While they were gone, Dorothy Bradford had
fallen overboard. She had been too weak to save herself. Once more, William was alone." (pg. 176–
177) Despite all the hardships that were thrown at him during his life, William Bradford, still
became a remarkable, well–known man. When many of us would be asked if we knew who William
Bradford was, we would quickly respond saying he was a great leader all throughout his life.
Although, what hardships in life and experiences did he have when he was a boy, which transformed
him into to such a greatly known man. Bradford Smith's Book, "William Bradford Pilgrim ... Show
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This made him rely on God all throughout his life when he was alone, discouraged and sad. God
comforted him in many ways by sending him friends at all different times and by simply giving him
a peace inside. All these smalls challenges and tragedies in life, which God made him face, were
only little tests of hardship to get him ready to rely on God even more in the upcoming years of the
travel to America. . As Romans 8:28 says, "We know that in all things God works for the good of
those who love him, who have been called according to his purpose." Even though in William's
brain he thought he had a horrible, stressful childhood. He needed know that God had a plan the
whole time and if God would have revealed that whole plan to him, William would have been happy
and amazed with all he did in his life. For all the deaths of his family members, which made him
rely on God spiritually. He was raised up strong, spiritually and physically which prepared him for
making the American settlement by tilling the fields, farm work and walking those miles to and
from, church and school. When he almost died in sickness but it was really getting him ready for the
horrible sickness that awaited him in the trip to America. And all the miles he walked, even in the
hard winter, to school every morning and night on his own accord, made it so that he was such a
great leader, which got him to be the governor of
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Langthwaite Short Walk
Beginning from the cosy village of Langthwaite, this short walk has outstanding views. Fine heather
moorland and sparkling streams help to compensate for the ravages of the lead mines and spoil
heaps.
Langthwaite is acknowledged as the capital of Arkengarthdale and lies on the road from Reeth to
Tan Hill. The road was constructed in c.1741 and it was one of the first turnpikes. Its original
purpose was to transport coal from the Tan Hill mines to be used in the smelting furnaces around
Langthwaite. Turnpike roads extracted tolls from travellers in order to pay for their building and
maintenance. They often took advantage of earlier routes, especially through valleys, which were
formerly toll free. Turnpikes were resented by the packhorse and ... Show more content on
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Bell pits were one of the earliest methods of mining for lead and other minerals. A vertical shaft was
sunk to a depth of about 20 feet (6m) before digging horizontally, along the vein. The ore and waste
were removed by a hand winch and a bucket. Due to poor ventilation, these workings could not
progress very far before the air became foul. There was also the danger of the tunnel collapsing. To
prevent this happening another bell pit was sunk close by. This procedure was repeated many times,
resulting in a line of bell pits spaced out along the length of the vein.
During our descent to Booze the scenery of Calver Hill, Fremington Edge and lower Arkengarthdale
rouses the senses. Booze is a small hamlet, consisting of just a few farms and cottages, but
unfortunately it does not have any licenced premises as its name implies. As a matter of fact, most
of its early residents were Methodists and so they abstained from the demon drink. In 1473 the
hamlet was known as Bowehouse, changing through the years to Booze. The original name means
'The house by the curve', the curve possibly referring to the curving hillside or perhaps to one of the
nearby
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Tim Tebow's Strength is Jesus Christ
¨When people see the work ethic, determination and the passion that I have, they're going to ask,
"What drives you? What is that?" Well, the answer is my relationship with Jesus Christ. that's just
what has always fueled me." (Rogeberg). As a tremendously skilled and dedicated athlete, Tim
Tebow uses his strengths to show Christ to people in a unique way. Tim Tebow demonstrates that
Christlikeness and courage are essential to being a strong leader. On a crisp summer day, August the
fourteenth, nineteen–eighty seven, a legend in football and leader in Christ was born, named
Timothy Richard Tebow. He was born into a small town in the Philippines called Makati City. Tim
Tebow's parents were originally from America but they moved to the Philippines to do baptist
missionary work (Whitaker, 5). In October of 1990 Tim Tebow's family of four other older siblings
moved to Jacksonville, Florida where Tim was raised. The Tebow's next door neighbor, Uncle Dick,
was like a true uncle to Tim. Most of his childhood memories were spent in Uncle Dick's household
(Whitaker, 7). You rarely see homeschoolers turn into NFL stars, but Tim Tebow was one. His
mother homeschooled him from kindergarten until senior year of high school. His mother and father
were his biggest influence to getting to know Christ ("Tim Tebow Biography"). Tim Tebow says
when he was seven years old he had a strange fear of getting into a wreck and not spending eternity
with God. With his parents help he accepted Christ
... Get more on HelpWriting.net ...
Identifying An Organism With A Biological Lens Essay
Spectrophotometrically Identifying Alfalfa Sprouts' Chloroplasts and Lysosomes Isolated in a
Sucrose Gradient
Introduction: Conceptualizing an organism with a biological lens involves understanding the
organism's components and how their individual functions characterize the overarching entity. For a
body, this means understanding organs and tissues. For a cell, this means classifying its organelles
and discerning their functions. Analysis of subcellular components has become a major focus in
biochemical studies, and it relies heavily on the fractionation of organelles. Fractioning and isolating
organelles essentially enables a scientist to elucidate organelle functions, which, as mentioned
before, adds definition to the all–encompassing image of a cell. Modern studies on this topic tend to
target organelles with somewhat ambiguous functions, like anammoxosomes and melanosomes. By
isolating these subcellular components and analyzing them, biologists have unearthed
ammoxosomes' role in filling a large part of the Earth's atmosphere with nitrogen gas (Neumann et.
al, 2014). In addition to this, other biological organizations have managed to sequester intact
melanosomes from cells, separating them from other items of similar densities, and study the
activity of V–Type ATPase proteins while they are in a subcellular membrane (Pelkonen et. al,
2016). The general procedure for executing an experiment like these involves lysing cells,
centrifuging their components, and separating
... Get more on HelpWriting.net ...
Wal Mart As A Positive Image
Sam Walton started Wal–Mart in 1962. The first store was in Rogers Arkansas. Daft (2008) have a
statement to Wal–Mart that, "Wal–Mart is the largest retailer in the United States" (p. 129). Wal–
Mart's website and financial statements disclose that the key values and beliefs include Wal–Mart's
claim to keep costs low for the customer and to build trust in its customer base ("Wal–Mart 2010
annual report", p. 16). Walton built Wal–Mart on three core values, including respect for the
Individual, service to Our Customers, and striving for Excellence ("Wal–Mart statement of ethics",
2010 p. 5). Definitely, Wal–Mart has created a positive image through its several years'
contributions. The paper will dis–cuss Wal–Mart's work in following ... Show more content on
Helpwriting.net ...
Walton once said that "a promise we make is a promise we keep," because they think the reason of
provid–ing superior customer service is to assure that Wal–Mart are so thankful about consumers'
trust and want to build a long relationship with customers. That is why they are always doing so.
We can also see the respect of privacy in the Wal–Mart's privacy policy. Their policy in–dicates that
how and why Wal–Mart uses, and shares personal information. For instance, when consumers take
an order online, Wal–Mart automatically collects personal infor–mation, including name, address,
phone number, card information.
In this technology–boosting world, they recognize the importance of keep the safety of customer's
personal information, because the information is so cheap. From the privacy policy page, we can
know that they have lots of people who take charge to protect the information. When consumers are
shopping on websites, or in the local stores, they use reasonable security methods. These methods
include security access controls or other physical security safeguards, information security
technologies and policies, procedures to help ensure the appropriate disposal of information (How
Do We Secure Your Person–al Information, n.d).
There are several examples, indicating that Wal–Mart pays a lot of attention on protecting personal
information. For instance,
... Get more on HelpWriting.net ...

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Sam Bradford Research Paper

  • 1. Sam Bradford Research Paper It seems that this isn't a great day for the Green Bay Packers. You know why? It's because the former No. 1 pick Sam Bradford has one of his best games right now. Even though that his career was on a roller coaster ride, Sam didn't give up and proved to them why he was picked #1 overall. This is the result when the Vikings beat the Packers in a complete upset. He started his first game with for the Vikings after his offseason was considered "tumultuous". It all began when he signed an extension with the Philadelphia Eagles, only that he was traded to the Minnesota Vikings afterwards. It's something that he really didn't expect, especially that he was the former #1 draft pick. The trade happened 8 days before the start of the season. He admitted that he was struggling to find his true form, until he has some swagger last night when he outsmarted and outplayed Aaron Rodgers. As for Aaron Rodgers, the reigning Most Valuable Player, he knew that the Minnesota Vikings are not to be taken lightly. This is why in every game, he wasn't confident enough ... Show more content on Helpwriting.net ... It all changed today after the regulation, as the Vikings upset the Packers, 17–14. As a result, they've moved to 2–0 record. It was believed that two–thirds of the team with this record can make the playoffs. However, there was a setback when the Vikings won over the Packers. On Jane Slater's Twitter account, she posted a video of Adrian Peterson, who suffered a nasty injury on his right knee during the game. He was fine standing up with crutches, on the team's locker room and it wasn't know when he will come back to play for the team. The Vikings have lost a guy who is really determined to win. As long Sam Bradford regains his true form with the Minnesota Vikings, expect that they're going to have a better future ahead. Not only that, they can contend for a playoff run and a possible championship match with a strong ... Get more on HelpWriting.net ...
  • 2. Lab Report : Bca Protein Assay Shrutika Mintri/ E2 Date : 09/17/2014 Lab Report #1 Project Title: BCA Protein Assay Purpose: BCA (Bicinchoninic acid) Assay otherwise known as the "Smith Assay" has the fundamental purpose of determining the protein concentration of the two given unknown proteins in the sample solutions. The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed in this Assay. Methods: (1) There are two methods using which the BCA Protein Assay can be performed– Test tube method and Micro plate method. The following experiment has been performed using the Microplate procedure. Vial Volume of Diluent Volume and Source of BSA Final BSA Concentration A 325 μL 325μL of Stock 1000μg/ml B 325μL 325μL of vial A dilution 500μg/ml C 325μL 325μL of vial B dilution 250μg/ml D 325μL 325μL of vial C dilution 125μg/ml E 400μL 0 0μg/ml= Blank 1. Preparation of Diluted Albumin (BSA) Standards: The following Diluted Albumin (BSA) Standards have been prepared using Bovine Serum Albumin (BSA) Standard Solution (2.0mg/ml) and the Diluent Phosphate Buffered Saline (PBS) having ... Get more on HelpWriting.net ...
  • 3. Walmart Is The Nation's Largest Business The first Walmart was opened on July 2, 1962 in Rogers Arkansas. It was the visionary work of Sam Walton. In 1967 Waltons family already owned 24 stores and had 12.7 million dollars on sales. By 1980 Walmart had already had 276 stores and employed 21,000. In 1992 Sam Walton said the company 's mission which was to save people money so they can live better. In 1993 walmart celebrated its first one billion sales for a week. In 2000 customers were allowed to shop online. By 2002 wal–mart had passed the 500 largest American companies. Walmart has spread to 27 different countries outside the United States. Now Walmart is the nation 's largest business in the world. It employs 1.4 million people with 5,239 locations within the United States locations. Walmart has had questionable ethical business practises in there stores. Low wages at Walmart locations have been a problem. A report from the National Employment Law Project states that the low prices at Walmart provided to the customers have a hidden cost to the workers. For Walmart to have it's low prices and to still have a profit they have to pay low wages to the workers. Its low wages have a negative effect to all the 2.2 million workers walmart has.This makes it harder for the workers to buy food, housing and other bills like the water and the light. Wal–mart has plumed wages in it's places of supply by hiring people outside the company to provide it's services that traditionally are performed within Wal–mart's warehouse. ... Get more on HelpWriting.net ...
  • 4. Rhetorical Analysis : Rhetorical Analysis Of Mickey... Rhetorical Analysis of Mickey through the Years From Facebook to billboards, our minds are bombarded with advertisements. According to a study conducted by Red Crow Marketing Inc., the average person sees "10,000 to 40,000" ads a day (Red Crow Marketing Inc). Among these advertisements are those that sell keepsakes and collectibles. The Bradford Exchange is a company that sells such astounding keepsakes for the everyday movie buff. The company has tactfully placed advertisements in magazines such as InTouch, which many busy mothers and grandmothers buy due to its inexpensive price. Although there are several Bradford Exchange advertisements throughout InTouch, there is one titled Micky through the Years. Mickey Mouse, a children's character, opens the market to parents because mothers will buy the watch to be like their children. From experience, I have seen mothers who become interested in what their children like to gain respect from the children, hence why the ad is targeted towards mothers of young children. More importantly, the marketers have skillfully designed the ad to highlight the "stunning" and "timeless" attributes of the watch (Bradford Exchange Ad). In addition, the ad also has used color and vivid wording to captivate the minds of those mothers who bought the cheap magazine for the gossip they do not get due to the busyness of their lives. Firstly, the mother's eyes will fall upon the watch's display strictly due to the colors that surround the watch, the ... Get more on HelpWriting.net ...
  • 5. Extraction and Characterization of Proteins EXTRACTION AND CHARACTERIZATION OF PROTEINS Abstract Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase, 1.03 g egg white albumin, and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict's test and the enzymes inverting action on sucrose was confirmed. Warburg–Christian Method and Bradford Assay were also employed to determine the protein concentration in the albumin and the casein samples. The concentrations for the albumin and casein samples were found to be 0.519 and 0.327 mg/mL, respectively based on Warburg–Christian Assay; and 6.5x10–3¬ and 1.9x10–2 mg/mL ... Show more content on Helpwriting.net ... As a result, the interactions between protein molecules exceed protein–water interactions, and the solubility decreases. Moreover, the differences in the amino acid sequence makes proteins differ in their salting in and salting out behavior. This is the basis for the fractional precipitation of proteins by means of salt. Most commonly used salt is ammonium sulfate because it is available in highly purified form and because of its high solubility. (Boyer, 2000) Proteins can also be fractionally precipitated by adjusting the pH, temperature and dielectric constant. Variations in pH also change the state of ionization of the functional groups, and hence, the net charge of the protein. Usually, the protein's solubility is at its least at the isoelectric point pI and increases on either side of the pI. At the pI, the net charge of the protein is zero and the protein molecules do not repel each other. As a result, protein–protein interactions are increased and the solubility is at its lowest. (Boyer, 2000) Meanwhile, if temperature is increased denatures the proteins. Proteins then unfold and the non– polar groups which were previously in the interior of the molecule become exposed. This leads to a decrease in the solubility of the protein in aqueous environment. Addition of ethanol, methanol, acetone and the like decreases the dielectric constant and thus decreases protein's solubility. (Boyer, 2000) The ... Get more on HelpWriting.net ...
  • 6. Unit 4 Equality and Diversity Essay NCFE Level 2 Certificate in Equality and Diversity Unit 4: Living in diverse communities Assessment You should use this file to complete your Assessment. The first thing you need to do is save a copy of this document, either onto your computer or a disk Then work through your Assessment, remembering to save your work regularly When you've finished, print out a copy to keep for reference Then, go to www.vision2learn.com and send your completed Assessment to your teacher via your My Study area – make sure it is clearly marked with your name, the course title and the Unit and Assessment number. Name: 1. Describe a range of appropriate investigative methods that can be used to explore diversity in a community. Range ... Show more content on Helpwriting.net ... Through this strategy, barriers to essential services would be unlocked and the potential for social integration enhanced. Additionally, it was hoped that building capacity in the asylum and refugee community, would increase opportunities for individuals to grow their skills, knowledge and competencies as well as their income earning potential. Olive Branch Trust – Olive Branch Trust was established in 1983 to invest in the local community of South Bradford, West Yorkshire. Over the years the Trust has grown considerably and now focuses on 2 main areas of work, namely the Family and the Youth. The Trust employs many people and these are helped by numerous skilled and dedicated volunteers In 2001 the Trust also became a Limited Company, and then in 2008/9 Community Interest Companies were formed to better deliver the services to our clients. There are now 2 companies namely E:merge CIC (Youth /Education Services) and BD4 CIC (services to the Family). Whereas the initial focus was our city of Bradford they now provide services to those further afield. Drug & Alcohol Counselling – Within Bradford there are several organisations offering help with drug and alcohol problems, organisations like the Council and the Health Authority and specialist voluntary organisations.
  • 7. They can give help from practical advice and information to counselling and ... Get more on HelpWriting.net ...
  • 8. Bradford Assay On Unknown Concentrations Of Proteins Bradford Assay on Unknown Concentrations of Proteins Taylor Coleman September 27, 2016 Lab Group 3 BIOL 1111: General Biology Lab Fall 2015 Section 107 Chad Perry Abstract Colorimetric assay is a process of determining a concentration of a solution based on absorbance of light. The purpose of this lab is to determine if the Bradford assay is an accurate way to determine an unknown concentration of two samples of protein. The Bradford assay is done by measuring wavelength of light passing through a cuvette filled with Bradford dye and concentrations of PBS and proteins. After the cuvettes are mixed they are placed into a spectrophotometer to measure wavelength. The wavelength given will be used to plot a standard curve based on concentration (x–axis) and wavelength (y–axis). The standard curve is then used to measure an educated guess on the concentrations of unknown protein concentrations. We hypothesized that if we use the Bradford assay and colorimetric spectrophotometry we can determine an accurate concentration of two unknown concentrations of proteins. The results of this lab failed to reject our hypothesis based on accurate measurements of protein concentrations. The standard curves are drawn with a linear increasing slope. The Bradford assay is an accurate way to demine the concentration of an unknown concentration. Introduction Colorimetric assay is a process determining the concentration of a chemical element or compound in a solution ... Get more on HelpWriting.net ...
  • 9. Wal Mart's Ethical Issues Although prices at Wal–Mart are considered as reasonable they are still having their own issues in relation to ethical issues and unethical practices consists of consumers were complaining about how they were not paying what they consider a fair prices for their groceries. Wal–Mart use to cover up what they were doing to their customers the advocate group followed the Food Chain Workers Alliance in order to investigate the root of the problem in the outsourcing ethical systems. Wal–Mart worker are currently complaining they are not being treated fairly because they are overworking their employees and they are not receiving enough pay based on their skills and qualifications they should determine how qualified their employees are for the job of their choice. Wal–Mart just followed existing law. Since the central contradiction of corporate social responsibility ethics is reduced to a matter of noblesse oblige, not human rights. Wal–Mart have been breaking the labor in which it is unethical practices because the labor department felt they were not being treated properly and the customer felt they were not giving the right quality of merchandise in which they paid for. According to report all outlets along with the suppliers are to fully abide to the law regulations in relation to the immigration labor, safe environment health. Wal–Mart was founded in 1962 in Arkansas by a mean named Roger's Sam in 2002 Wal–Mart had become a very popular store for consumers to shop however ... Get more on HelpWriting.net ...
  • 10. Corporate Social Responsibility : Walmart Corporate social responsibility has two parts. First, CSR means a company should seriously consider the impact of its actions on society. Second CSR, requires that a company or individual be responsible for how their actions impact the society. Walmart is a corporation that some can defend does display CSR, while other can defend that it does not. Walmart was the epitome of what a retail chain store should be. Sam Walton took what he learned from the JCPenny model and transformed it into something better. He was responsible for the social responsibility innovations of the "Buy American" plan and the "Environmental Awareness" campaign. These innovations display how CSR was displayed by Walmart. The "Buy American" plan showed corporate responsibility because when Walton discovered that his company outsourcing was contributing to American job loses he devised a plan to showcase the domestically purchased merchandise that Walmart was now stocking. This was an ideal way to incorporate the second definition of CSR into the corporation because Walmart was considering the impact it had on the society. However, the corporate responsibility Walmart once tried to incorporate was lost when it became one of the largest purchasers of goods made over– seas, which in turn forced US manufacturers out of business. In early 2013, Walmart announced they would be buying American again, however, it is a struggle to find vendors that will meet Walmart's price points. Walmart could have avoided this ... Get more on HelpWriting.net ...
  • 11. Essay about What Really Happened During the First... There can be countless records of the same event, and while many times each record is different, there are also incidents in which one needs to know every record to piece together what really happened. The story of the first Thanksgiving takes place in 1621, and is generally known as a meal that was shared between the Pilgrims and the Indians. For the Pilgrims, this was a harvest festival, much like one they would have had in England. For the Indians, it was a show of hospitality from the Pilgrims, similar to what they would expect from visiting another Indian tribe. With being told only that, one can already see that there are already two very different histories of the same event recorded. Of greater interest, perhaps, are the different ... Show more content on Helpwriting.net ... The History Channel's Desperate Crossing tells the story of the Pilgrims, their journey to Plymouth, their horrible first year, and finally, the first Thanksgiving. Desperate Crossing follows the accounts of both Winslow and Bradford fairly well, but begins to deviate slightly. They show that the Pilgrims and Indians played games, drank, and raced, and competed together, some of which are only vaguely and briefly alluded to by Winslow, and never by Bradford. The History Channel also shows a much more tense situation than the impression given from Bradford and Winslow. According to the History Channel, everything from Massasoit showing up with his ninety–some men and the Pilgrims firing their arms is all a display of strength from both sides. The History Channel obviously takes some liberties in their presentation of history. Winslow and Bradford do not mention anything about the clothing worn, if there was any drinking involved, if there was silverware, where the feasts were held, and many other seemingly trivial details. Desperate Crossing makes some assumptions on what took place in 1621, but it does quote both Winslow and Bradford word for word. While we're shown a picture that may or may not actually represent what took place at the first Thanksgiving, at the very least, we're given Winslow's and Bradford's descriptions of it. The History Channel used what was already known from the eye– witness accounts combined with what ... Get more on HelpWriting.net ...
  • 12. The Case Of Wal Mart When we hear someone say they need to go to the grocery store, what is the first place that comes to mind? For most of us, it is Wal Mart. From its market end, to its home and beauty supply, all the way to its lawn and garden department, Wal Mart has been ordained one of the most profitable companies in the entire world. But it did not start out that way. With a dream, determination, leadership, and some sweat, Sam Walton, made a small mom and pop store into the multibillion dollar company it is today. On March 29, 1918, Samuel Moore Walton was born to Thomas and Nancy Walton of Kingfisher, Oklahoma. As a young boy Walton and his family moved locations and found themselves living in the small town of Columbia, Missouri. Growing up, Walton excelled at school, making the top grades of his class. He was also known for his athletic ability, and in high school was the quarterback for his high school football team (biography.com). When Walton graduated high school in 1936, he was voted "most versatile boy" (biography.com). Little did anyone know that that most versatile boy was going to achieve great success in just a few short years. After graduating high school, Sam decided to further his education and attended the University of Missouri. He graduated in 1940 with his degree in economics. Shortly after graduation, Walton joined the army at the mere age of 24. He served until 1945 as Captain of an intelligence unit (biography.com). Upon returning home ... Get more on HelpWriting.net ...
  • 13. The Spectrophotometer Determination of Protein... The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X–100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs, et.al, 1959). Spectroscopy is the use of electromagnetic radiation by a sample in order to identify components of the sample (qualitative analysis) or to measure the amount of material in that sample (quantitative analysis) (Tombs, et.al, 1959). Absorption is a process in which ... Show more content on Helpwriting.net ... MATERIALS AND METHODS The BIORAD dye was firstly prepared by dissolving 0.225g of Coomassie Brilliant Blue G–250 (Merck, South Africa), in 67.5ml of Methanol (Riedel–de–Haёn, Germany) and 291ml of 85% (w/v) Phosphoric Acid, diluted to 450ml with dH2O. Six test tubes were labelled SC1–6 and the 0.1mg/ml stock of Bovine Serum Albumin (Roche Diagnostics, Germany) and dH2O was pipette into their respective test tubes according to Table 1. Table 1: Table of the BSA and dH2O volumes to be pipette and placed in the spectrophotometer to obtain absorbencies for the standard curve Test Tube Number Total Volume (μl) Protein (BSA) Stock (μl) H2O (μl) Amount of Protein (μg) 1 2400 0 2400 0 2 2400 50 2350 5 3 2400 100 2300 10 4 2400 150 2250 15 5 2400 200 2200 20 6 2400 250 2150 25 600μl of the BIORAD dye agent was pipette into each test tube making up the volume to 3000μl. All the test tubes were then covered with Parafilm and placed in the Vortex (Lab Pro cc) for a few seconds and they were incubated at room temperature for 5–60 minutes. Simultaneously another set of test tubes were labelled X1–8 and pipette with 0.1mg/ml Lysozyme stock (Merck, Germany), BSA, 1% (w/v) Sodium Dodecyl Sulphate prepared with dH2O, 1% (v/v)
  • 14. ... Get more on HelpWriting.net ...
  • 15. The Organization's Stand, Diversity Audit Conduct Audit In order to perceive about the organization's stand, diversity audit is vital and the separation that is needed to cover its objectives area. Notwithstanding this, implementation and planning diversity audit is a crucial procedure and there are some key focuses that Sam 's West, Inc. (working together as Sam 's Club) ought to consider with a specific end goal to direct differing qualities review in a compelling way (Oberoi, 2014). These are portrayed as beneath: The audit must cover every one of the gatherings of workers and HR procedures of the firm on account of diversity impacting individuals of the firm. Besides, it ought to concentrate on both quantitative and qualitative information. Sam 's club ought to hope to characterize the principle reason for auditing. For case, there might be various motivations behind auditing like to deviation from the general procedure, to gauge representative input, survey business effect of assorted qualities mediation, future strategy, and so on. Besides, Sam 's club ought to likewise confirm that how every now and again it needs to direct the review. Primary and secondary research likewise helpful so as to direct audit in a viable way. Primary and Secondary Research Primary Research Primary research is an examination or the gathering of information firsthand, or by an outsider contracted particularly for the firsthand party. Research you do yourself that has not been done some time recently (Kleynhans, 2013). ... Get more on HelpWriting.net ...
  • 16. LDH Purification lab Report PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine the presence and purity of LDH. The cells were initially disrupted and proteins were solubilized. LDH was purified from the ammonium sulfate precipitated protein mixture by affinity chromatography and its activity was studied by ... Show more content on Helpwriting.net ... 10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted. Protein Assay: The Pierce BCA Protein Assay (Thermo Scientific) is a detergent–compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein concentration. A series of standard solution of Bovine Serum Albumin (BSA) ranging from 0–2000 µg/ml was prepared from a stock solution of 2 mg/ml BSA. 25ul of diluted crude (1:500, 1:250), desalted (1:100, 1:50), and 6 peak fractions from cibarcon blue column (1:10, 1:5) were loaded in microplate along with 175ul of BCA working reagent. Microplate was incubated for 30min at 370C and then the absorbance was measured at 562nm. Results/Discussion The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of techniques including homogenization, ammonium sulfate precipitation, dialysis, and affinity chromatography. Activity and Protein assay were used to track the overall amount of LDH present in the samples. Crude Extraction: Chicken muscle tissue was homogenized in a blender with cold extraction buffer in order to lyse cells, releasing LDH into ... Get more on HelpWriting.net ...
  • 17. Protein Concentration Lab Report Figure 3. Bradford Standard Curve using 10mg/ml BSA Protein. 10mg/ml BSA protein stock was serially diluted to cover a 5–2000µg/mL concentration range. 50 µL of dilutions were added to 1.5mL Bradford Reagent, left at room temperature for 5minutes, and assayed at 595nm, against a blank of 50 µL DI H20 and 1.5mL Bradford Reagent.1 ________________________________________ From the Bradford standard curve equation, the protein concentration of the crude lysate was calculated to be 0.1402 µg/mL, and the protein concentration in Fractions 1, 2 and 3 was found to be 11.5038 µg/mL, 11.5038 µg/mL, and 26.6553 µg/mL, respectively. Although Fraction 2 appeared to have the most intense yellow coloring, Fraction 3 had the highest protein concentration. ... Show more content on Helpwriting.net ... The Stokes radius of a molecule is the effective radius a molecule has as it moves through a solution. Molecular weight of a molecule is sometimes correlated with the Stokes radius of a molecule, and thus the elution trend in gel–filtration, but this is not without exceptions. Thus, Stokes radius is a better predictor, because although two molecules may have the same molecular weight, a longer, more branched molecule will have a larger Stokes radius than a smaller molecule.1 In the separation of blue dextran, cytochrome c, and potassium chromate, the molecular weight of the molecules were proportionate to their molecular sizes. The largest molecule, blue dextran, eluted first, followed by cytochrome c, and then the smallest molecule potassium chromate. Blue dextran has the largest molecular weight, and potassium chromate has the smallest. The results for elution order are in accordance with what the theoretical, predicted results were. The efficiency of the separation was not as ideal as would be predicted on the basis of molecular weights. The molecular weights of the molecules are 3 orders of magnitude different, and should produce well–resolved peaks.1 Experimental visual results show peak overlapping around 2mL and 3mL, as the colors were mixed at these elution volumes. This peak overlapping may be attributed to peak broadening. Peak broadening has many causes, but of particular ... Get more on HelpWriting.net ...
  • 18. Causes Of Civil Disobedience Although civil disobedience rarely accomplishes the change that it demands directly, it does create change indirectly through raising awareness and support. Civil disobedience works by relying on the reaction of governments/societies after individuals rebel to rally support for the protest. If the government/society violently crushes the rebellion, as in the case of Antigone, then the civil disobedience wins by creating pity and sorrow for the rebellion. However, if the government/society does not react, then the individuals are still successful by being allowed to get away with acts of civil disobedience which spread their cause. Despite the tragedies surrounding the American Civil Rights Movement, change could not have been made without the violent reaction to the movement. Rather than stopping or slowing desegregation as the violent reactions intended, they only created martyrs for which the Civil Rights Movement could rally around. Famously, J. W. Milam and Roy Bryant kidnapped and murdered Emmett Till, a fourteen–year–old African boy, on account of flirting with a white woman. Then, the guilty party was found innocent despite overwhelming evidence otherwise. Although this incident may have been notable on its own, it became a very powerful motivation for the Civil Rights Movement through the confession/arrogance of the kidnappers, and the work of Emmett Till's mother, Mamie Till. Infamously, Roy Bryant and J. W. Milam sold the story of Emmett Till to reporter William ... Get more on HelpWriting.net ...
  • 19. Absorbance Values Abstract: Claims were made that manufacturers had improperly labeled protein concentrations on their products. Concentrations of these three different protein–containing liquids, whole milk, soy milk, and a protein solution were determined using a Bradford dye reagent and a spectrophotometer to measure the absorbance of each. Absorbance of a set of standard known protein concentrations from a range of 0.125 mg/ml to 2.000 mg/ml were measured using the spectrophotometer at a set wavelength and this data was plotted. The equation generated from the best fit line of this data was used to determine the unknown concentrations of the three different samples. These values were then compared to the protein concentrations obtained from the corresponding ... Show more content on Helpwriting.net ... This would indicate that either the food labels or the calculated data are incorrect. The data was dependent on a linear relationship between absorbance and protein concentration. However, the linear relationship was based on only seven data points, two of which were omitted due to inconsistency, and was only representative up to 2.000 mg/ml protein concentration. Because the data sample was small and covered a small range of concentrations, the food label concentrations cannot be determined as inaccurate. The more likely cause would be that the linearity assumption of the correlation between the absorbance and the protein concentration is ... Get more on HelpWriting.net ...
  • 20. Phl/458 Famous Thinkers Essay PHL/458 October 8, 2012 Famous Thinkers While thinking of computer software, you begin to think of Microsoft. In fact if you use a computer, chances are that you will have some type of program on there that is developed by Microsoft. Bill Gates is the chairman and co–founder of Microsoft Corporation, the worldwide leader in software, services and solutions that help people and businesses realize their full potential. When it comes to discounted variety stores there are not many stores that compare to Wal–Mart. The founder and former chairman of Wal–Mart Stores Inc., the world's largest retailer, is Sam Walton. The innovative thinking of Bill Gates and Sam Walton has changed the business world. With Bill Gates' contributions to ... Show more content on Helpwriting.net ... Guided by a belief that the computer would be a valuable tool on every office desktop and in every home, they began developing software for personal computers. Gates' foresight and his vision for personal computing have been central to the success of Microsoft and the software industry. Under Gates' leadership, Microsoft's mission has been to continually advance and improve software technology, and to make it easier, more cost–effective and more enjoyable for people to use computers. The company is committed to a long–term view, reflected in its industry–leading investment in research and development each year (Microsoft, 2011). Samuel Moore Walton was born March 29, 1918 in Kingfisher, Oklahoma. His dad, Thomas Gibson Walton, was a banker, farmer, farm loan appraiser, and real estate and insurance agent. Sam Walton had an entrepreneurial spirit from an early age and after graduating from the University of Missouri at Columbia in 1940, he began his lifelong career in retail as a management trainee at J.C. Penney. He joined the military in 1942 where he served until 1945 when he was released. While in the military, Sam Walton's wife decided that she wanted small town living so they moved to Newport, Arkansas where Sam Walton purchased a Ben Franklin five–and–dime franchise. By the early 1960's Walton owned 16 Ben Franklin stores in Kansas, Missouri, and Arkansas but he was not seeing the profits that ... Get more on HelpWriting.net ...
  • 21. Material And Methods : Bm Mscs Cultivation Material and Methods: BM–MSCs cultivation: BM–MSCs were obtained from Institute Pasture of Iran and were cultured in Low Glucose Dulbecco 's modified Eagle 's medium (Low Glc–DMEM) (Gibco Invitrogen, Karlsruhe, Germany) with 10% FBS (Gibco Invitrogen, Karlsruhe, Germany) and 1% penicillin and streptomycin (Sigma–Aldrich, Schnelldorf, Germany). Then, BM–MSCs were incubated in humified incubator at 37˚C and 5% CO2. Cell's medium was replaced every two days. U266 cell line: Confirmed U266 cells, a human myeloma cell line, were purchased from Institute Pasture of Iran. U266 cells were grown in RPMI–1640 (Gibco Invitrogen, Karlsruhe, Germany) with 10% FBS (Gibco Invitrogen, Karlsruhe, Germany) and 1% penicillin and streptomycin (Sigma–Aldrich, Schnelldorf, Germany) in T25 flask culture (Orange Scientific, Braine–l 'Alleud, Belgium). Then, U266 cells incubated in humified incubator at 37˚C and 5% CO2. The medium was changed every day during all steps of experiments and Trypan blue staining was used for viability analyzing. BM–MSCs derived C.M preparation: The BM–MSCs were cultured in T25 flask culture (Orange Scientific, Braine–l 'Alleud, Belgium) with DMEM medium and incubated for reaching to 60–70% confluency. In the next step, DMEM medium was replaced with RPMI–1640 medium without FBS and was incubated for 72 hours (hrs). After incubation; medium was collected, cells and debris were removed by centrifugation. Supernatant was concentrated by 10 kDa MW cut–off ultrafiltration ... Get more on HelpWriting.net ...
  • 22. Globalization of Walmart Globalization of Walmart Mg 460 George Rideout Lisa Baron November 10, 2014 The location of the first Wal–Mart in the Fortune Global 500 for the year of 2001 to 2002 turnover of 219.81 billion dollars. Wal–Mart is the largest company in the retail in the world. The company was much larger than its competitors in the United States – Sears Roebuck, Kmart, JC Penney and Nordstrom combined. In 2002, Wal–Mart operates more than 3,500 discount stores, Supercenters and Sam's Clubs in United States and over 1,170 stores in major countries around the world. The company also sells products online via the website, www.walmart.com. Wal–Mart is one of the largest private employers in the world, with the use of force about 1.28 million. The ... Show more content on Helpwriting.net ... Wal–Mart failed to grasp the consumer and retail environment in Japan. With a population of 127 million, the highest per capita income and the second largest economy in the world, Japan is a very smart market for retailers. The opportunity exists, but there is much more research and planning that needed to be done before expansion began. Instead of adapting business operations to the Japanese culture, the company essentially assumed the Japanese would readily adjust to Wal–Mart's. For example, in Japan there is a much larger need for local store customization. Consumer buyer behavior is much different than in the United States, with purchasing patterns and product selection varying greatly between regions. They have a trend to buy smaller quantities in regular intervals rather than the more American idea of "stocking up." Similarly, the concept of large retail stores is foreign. Retailers with the highest growth rate are small specialty stores; quite the opposite of Wal– Mart. The culture tends to buy more fresh produce than pre–packaged goods as well. Lastly, the Japanese view high price as equaling high quality. This mentality causes them to purchase forty percent of the world's luxury goods annually. Packaging and appearance of goods play a huge role in their purchasing decisions. When looking at Wal–Mart's product selection, it is obvious they do not usually cater to luxury–brand customers. All of these cultural misunderstandings lead Wal–Mart ... Get more on HelpWriting.net ...
  • 23. The Importance Of A Good Business Communication Network... Analysis of Wal–Mart Company Patricia Diggs INF220: IS Principles (CLH1448A) Instructor: Torre Wilson December 15, 2014 Computer technologies and inventions have revolutionized the way business is done in our current age. I believe it would be safe to say that no business can survive today without computers, primarily for their fast communication channels available. A good business should have a good communication network infrastructure set up internally for fast communication between the workers and externally to suppliers, and their market. So what are the characteristics of a good business communication network design? Before I get into that I would like to choose a company that I think has a good business communication ... Show more content on Helpwriting.net ... Wal–Mart has employed over 2.2 million associates across the globe and about 1.3 million in the USA (Wal–Mart company website, 2014) Maintaining communication in such a huge work force is a daunting task. Wal–Mart communication network reaches all its employees from the cashier to the CEO. The company's progressive innovation in their technology has been the key to their global success. Wal–Mart's technological investments have kept the company's products at low prices and available and accessible to its customers. Wal mart has outsourced their technical communication so that they can concentrate more on the business itself. They have also outsourced their database, which is the world's largest commercial database at 7.5 terabyte, to NCR Corporation. They also use software that helps the management to more effectively communicate with other departments for instance the accounts and human resources departments. Neo Vista Company who tailored the software for the requirements of the NCR database environment provided for this software. This type of access to data is known as data mining where any employee of Wal–Mart could access the database and get key information about any item sold or is available for sale at any time the information is required. This has proved ... Get more on HelpWriting.net ...
  • 24. Bus 110 : Fall 2015 BUS 110 – Fall 2015 Final Project, due 12/9 This assignment allows you the opportunity to examine a publicly traded company. Required: 1. What is the name and mission statement of the company? a. The company's name is Walmart and their mission statement of the company is ( " We save people money so they can live better") 2. Describe the company's main product(s) or services. Where do they sell their product? Are they an international company? Where are their stores? a. The company's main products are clothes, toys, sporting goods, and groceries. They sell their product to the United States. They are an international company. They have stores in Alaska, Japan, Canada, United Kingdom, China, India, Nigeria, United States, Mexico, Ghana, Brazil, Uganda, Kenya, Tanzania, Mozambique, Zambia, South Africa, Namibia, Botswana, Brazil, Argentina, and Chile. 3. A history of the company telling when it was founded, where and by whom. In most companies you will find an interesting story on the origins of the company. Summarize major events and tell how those events affected the company. A timeline may be a great way to show major events. Walmart was founded in 1962 by Sam Walton in Rogers, Arkansas. Some major events in this company are Timeline 1950 On May 9, 1950 Sam Walton opened up Walton's 5&10 in Bentonville, Arkansas. In 1962 Sam Walton opened the first Wal*Mart in Rogers, Arkansas In the past fifty years, Walmart has grown rapidly, they have 2 million people ... Get more on HelpWriting.net ...
  • 25. Walmart Wal–Mart study Case#1 1. What threats and challenges is Wal–Mart currently facing? From the beginning, Walmart did not have many threats. However, not only the competition is different, several global retailers such as Target, Carrefour, Costco, and Amazon, are working hard to keep efficiency. They are trying to work together to shrink the prices difference between them. Walmart has facing difficulties from every single angle. Not only the company has internal labor relation problems, but also it has some external threats from its competitors. The company must work hard to get possible solutions against its competitors, and to solve any internal problems regarding its labor relations. Even though Walmart does not have any problems ... Show more content on Helpwriting.net ... However, in 2008, there was a change from $8.63 to 10.83 per hour. Managers at Walmart do not respect employee's rights, and they sometimes treat employees in a way that is against the law in the United States. Managers even sometimes force employees to work off the clock and skip lunch, and it is again against the law. The CEO needs to pay attention of such a situation. Because of the labor– related issues, there were several lawsuits against the company in 2008 in the following States: Colorado, Minnesota, Oregon, etc. There was also a gender discrimination situation where Women claimed in 2007 they were not treated fairly by the company. Even though people consider Walmart the company that employs more than 1.4 employees, it does not pay good salary to its employees. It is against the law to treat people in such a way. In 2002, Walmart used a type of competition that was not normal 3. Wal–Mart's Board discussed proposals to meet (some of) these challenges at a board retreat in 2005. Discuss these initiatives as outlined in the "Supplemental Benefits Documentation: Board of Directors Retreat FY06" See also Wal–Mart health cost strategy NYT 26Oct2005. Was additional damage done to Wal–Mart by the leaking to the public of the Board Benefits Strategy document? Challenges are considered the biggest issues for the Wal–Mart company. Even though there advantages, disadvantages ... Get more on HelpWriting.net ...
  • 26. Walmart Is A Multinational Retail Company Walmart Organization Walmart is a multinational retail company that runs a network of more than 11,000 discount retail stores as well as warehouse stores distributed in more than 27 countries. Its headquarters are located in Bentonville, United. It ranks as the world's leading private employer with over 2.2 million employees serving in different countries' retail stores. Walmart operates as a family owned organization that is managed by the Walton family. The Walton siblings own more than 50% of this company through managing the Walton Enterprises as well as through individual holdings (Charles, 2014). Walmart is equally ranked among the highly valuable companies, in terms of market value, as well as the biggest grocery retailer where it generates more than 51% of its sales from the grocery business. This paper explores Walmart's operation management with regard to supply chain characteristics, global business operations, production processes, the company commitment to quality and excellence, inventory methodologies, operational planning and movement towards lean processes (Massengill, 2013). Walmart Organization History and background information Walmart's history dates back in 1945 when Sam Walton bought an outlet of the Ben Franklin retail stores with the aim of selling products at a relatively low cost, so as to generate huge sale volumes and make profits at a low margin. Although this portrayed the purchased outlet as a crusade for most consumers, ... Get more on HelpWriting.net ...
  • 27. Walmart Mart 's Impact On The United States In 1950, Sam Walton obtained a little store from Luther E. Harrison in Bentonville, Arkansas called Walton 's 5 & 10. Walton 's 5 & 10 is currently called WalMart Visitor 's Center in Bentonville, Arkansas. In 1962, author Sam Walton opened the first WalMart store situated in Rogers, Arkansas. Sam Walton was propelled by other rebate office chain stores. By keeping his retail costs lower than alternate contenders stores he found himself able to open his second WalMart store that year in Rogers, Arkansas. There were an aggregate of 24 stores in the condition of Arkansas by 1967 with profit of $12.6 million in deals. In 1968, the first store was opened outside of Arkansas in Claremore, Oklahoma and Sikestone, Missouri. WalMart extended ... Show more content on Helpwriting.net ... "Because of this staunch hard working attitude and commitment to client care, WalMart asserted from the get–go that a formal morals system was pointless in light of the fact that the organization had Mr. Sam 's morals to take after" (Ferrell, Fraederich & Ferrell, p. 408). WalMart is known not the biggest retailer on the planet additionally known for low advantages and wages. This is a moral issue that was tended to. It was said that WalMart needed to procure healthier and low maintenance specialists. This was terrible press for WalMart and they needed to do something to enhance their association with worker stakeholder 's. Making the moves to enhance medical advantages and wages is the thing that the organization chose to do. In spite of the fact that WalMart improved upgrades by actualizing social insurance and better wages despite everything they missed the mark when they chose to diminish their workforce. This was bad on the grounds that client 's are expecting extraordinary client administration and with constrained representatives it is difficult to give great client administration. Another moral issue that WalMart has had press on was "Who 's Responsible for the WalMart Mexico Scandal?". This story hit the New York Times in April 2012, expressing that WalMart was included in a pay off in a Mexican auxiliary. It was purportedly said that WalMart ... Get more on HelpWriting.net ...
  • 28. Sustainability Initiatives A Demonstration Of Corporate... What are the cost benefits for corporations leading sustainable efforts in their communities and should corporations be financially responsible for the environmental disturbances that they cause, be it directly or inadvertently? More specifically are the efforts of Wal–Mart to implement sustainability initiatives a demonstration of corporate social responsibility or just greenwashing? Relevant to these questions we hypothesis that despite the multitude of efforts that are resulting in some groundbreaking policies for an Oligopoly such as this, that the fact that they still source, manufacture and distribute products in a manner that is unsustainable which makes these collective efforts an example of "social" greenwashing. Investopedia ... Show more content on Helpwriting.net ... Is this truly a serious initiative for Walmart to use their size for good or is this just greenwashing? Since their CSR plan announcement Walmart has been making progress towards achieving those goals but many don't think it's enough or realistically sustainable for the long run. Walmart has received backlash for their slow adoption of renewable energy, it's cheap products, and it's little progress on their sustainability index (Gunther, M., 2011). One of Wal–mart 's initiatives intended to address some of their inimical externalities is to convert to 100% renewable energy to power their stores. An article published in 2011 stated that Walmart was powered by only 2% of renewable energy and the ILSR came out with a report stating less than 2% of renewable energy powers Walmart stores. People were not impressed with this minimal progress and pointed out that other companies like Starbucks and Kohls use more renewable energy than Walmart. It was also pointed out that Wal–mart is a huge multinational corporation and that a process like this will take time, especially if they 're trying to avoid raising their costs, which in turn would not benefit the customers. However, in 2013 it was reported that Wal Mart 's 10,800 stores are powered by 21% of renewable energy, which is a decent amount of growth from ... Get more on HelpWriting.net ...
  • 29. Bradford And Lowry Assay Lab Report The Bradford and Lowry assay are two color changing proteins with similar processes. Color changes of the solution can be measured using a device called a spectrometer. In lab, the two assays can be used to determine the amount of protein in a solution. This can be done in an experiment by reacting a specific reagent with amino acids or peptide bonds present in the proteins. This produces a color change in the solution depending on the amount of peptide or amino acid reactions with the reagents. This color change can then be measured using a device called a spectrophotometer. The Bradford assay is a one– step process, which involves the Coomassie dye reacting with the amino acids arginine and lysine. It under goes changings in acidic conditions ... Show more content on Helpwriting.net ... The type of amino acid sample affected the ability of the reagents to react with the peptide bonds and amino acids. It also affected the amount of amino acids and peptide bonds in the protein. Each of the four proteins used in the experiment had the same concentration, which was reflected in the accuracy of the standards. In relation to the Bradford reagent, the amount of reactions that occurred depended on the amount of arginine and lysine amino acids present in the protein. In relation to the Lowry reagent, the amount of reactions that occurred depended on the amount of tryptophan and tyrosine amino acids in the protein and the number of peptide bonds linking the amino acids. The absorbance measured in the spectrophotometer was affected also by the differences in amino acid compositions and the ability of the reagents to react as a result. Since all the protein standards had the same concentration of 0.5 mg/mL, it allowed for the accurate comparison of the absorbance, mean, and standard deviation of each of the ... Get more on HelpWriting.net ...
  • 30. Case Study Walmart Allison Whitted Kaplan University Case study paper Wal–Mart in China GB520: Human Resources Management Introduction In this case study I will be discussing the company Wal–Mart Stores: Every Day low Prices in China. By the time 2005 rolled in China has become the most prominent country to set up a branch for stores. China had a huge open market and it was time for the company to take advantage of the opportunity that was happening in China. Wal–Mart has become the world's largest retail chain. This can only mean one thing and that is expansion. The strategic move to expand the company chain stores to China was based off of the following factors. First factor was China size as far as land, population. They also had a booming middle ... Show more content on Helpwriting.net ... According to the article experts says that Wal–Mart customers saved at least 15% on a typical cart of groceries (Farhoomand, A. F., Wang, I., September, 2008). The major issue for Wal–Mart was how to salvage the investment they made in China. They had these entire great strategic plan that worked in other countries that seemed to have done great. By 1996 the company decided to move a branch into China they started with a supercenter and a Sam's club. The business did not take off the way they hoped and planned. The company started to lose money since it opened stores in China. The company thought that the problem was that they had only one store in such a huge market. The fact of the matter was that the Chinese government turned down the company request to expand further in different parts of the country. The government was not convinced that Wal–Mart would be successful with all the new competition that was being implemented in other areas. Conclusion Wal–Mart learned that businesses in other countries may impact the benefit of an investment and is also learning that it's going to be trail and error. They can only hope that they can endure their place there so that they can have the potential to grow as a brand. I recommend that the company continue to research and discover what is in those local stores that are driving their consumers to keep coming back for more. References Farhoomand, A. F., ... Get more on HelpWriting.net ...
  • 31. Walmart Management Management Paper on Walmart Wal–Mart Stores: Managing Diverse Organizations By Angela L. Farrish Webster University Ms. Amy Thenor December 2011 Wal–Mart In 1970, Sam Walton the founder of Wal–Mart implemented an extremely significant warehouse distribution system. This new system evolved into Wal–Mart Stores Inc. and in that same year the company was publicly traded on the New York Stock Exchange. The company began to expand extremely rapidly throughout the 70's. During this period Wal–Mart started incorporating pharmacies and auto service centers into its stores which incited even more growth. By 1979, there were 276 Wal–Mart stores in 11 states. During the early 1980's Wal–Mart's ... Show more content on Helpwriting.net ... This motivational strategy allows employees to benefit from their performance which will in turn make the company's overall performance better. Home office management associates are eligible to receive additional compensation through stock options. Associates can become shareholders through the Associate Stock Ownership Plan. Wal–Mart also contributes 15% each year toward associate stock purchases. This contribution is capped at $1,800 each year. Since the typical Wal–Mart employee only makes between $6 and $7.50 per hour, most are unable to afford the stock options and only one in fifty associates participate in the program. Wal–Mart associates also receive 10% off selected store merchandise and are offered discounted child care through four national providers (Peterson Coleman). These benefits help Wal–Mart keep its best and brightest employees. Diversity Challenges Problems in businesses conducted across cultures often arise when individuals from one culture are not able to understand culturally determined differences. The most common difference is the language barrier if individuals are not fluent in the language. With individuals, who don't speak the same language, the use of nonverbal cues can explain what is trying to be communicated. Nonverbal cues and behaviors vary from one culture to another. For example, people from the U.S. are taught to use direct eye contact when communicating; in many
  • 32. ... Get more on HelpWriting.net ...
  • 33. The shape and magnitude of the UV spectra depends on the... The shape and magnitude of the UV spectra depends on the composition of amino acid in each protein. Due to the aromatic amino acid residues in the protein, the observed UV absorbance was mainly in the 240 nm to 340 nm region. In Figure 1 to 3, the maximal absorbance of each protein was approximately at 280 nm. The difference in magnitudes of the peak observed was linked to the differences in the amino acid contents in each of the proteins. The peak of lysozyme was greater than those of BSA and gelatin, because lysozyme has a greater number of tryptophan residues. Lysozyme has six tryptophan residues, whereas BSA and gelatin has two and zero, respectively (Department of Chemistry, 2014). Lysozyme has three times more tryptophan residues ... Show more content on Helpwriting.net ... In order to examine the variation in sensitivity of lysozyme, gelatin, and BSA, the absorbance reading was obtained using the Bradford and BCA assay Gelatin is observed to have a lower absorbance reading than lysozyme in only the Bradford assay, while in the BCA assay gelatin was observed to have about the same absorbance as lysozyme. In Bradford assay, the color yield for proteins with higher content of tryptophan, tyrosine, or cysteine residue, will be higher. The observed protein sensitivities did not correlates with the amino acid content in the Bradford assay. As presented in Table I, the mole fractions of lysine and arginine in BSA were 10 mole percent and 4 mole percent, respectively. The sensitivity should have been higher in BSA than in the Bradford assy. However, the observed sensitivities did correlates with the amino acid contents in the BCA assay. Bradford assay would be ideal if an accurate estimation of a newly isolated protein using BSA as a standard. With the data obtain from the Bradford assay, it shows that it was more sensitive and quicker, also it had less interference from substance that were possibly present in the protein solution, expect for the interfering substance SDS. The interference of RNA and ME in the Bradford assay was compensated by using the blank, because the difference between the addition of the protein and the non–protein blank ... Get more on HelpWriting.net ...
  • 34. Football : The Causes Of A Football Fire In Football One of the main reasons why I got interested in this event was that the fire took place during a soccer game. Soccer is by far my favorite sport to play and to watch. So when I heard about this tragedy, it got my attention because I have always been a big soccer fan. It's hard to understand that fans came to watch an exciting soccer game, but then 56 people ended up dead. This is why I was intrigued by this event and how this could possibly have happened. Valley Parade stadium in Bradford City, West Yorkshire, England goes down as the worst fire disaster in football (soccer) history. On May 11, 1985, what started out as a wonderful celebration ended in 56 deaths and 265 injuries. So many people died that day and it should not have happened. There were so many warning signs that were ignored by the owners which resulted in 56 deaths ranging in ages from 11 to 86. The stadium design, and the materials used as well as the lack of planning all contributed to this disaster. This fire changed how football stadiums are designed to ensure the safety of the fans. On Saturday afternoon, 11,076 fans arrived at the Valley Parade stadium to watch Bradford City versus Lincoln City. Before the start of the game, Bradford City was presented the championship trophy in their final Division Three game. This was the team's first Division trophy in 56 years. It was an exciting time at the game and for the city. A little bit before half–time, with the score 0–0, some smoke could be seen coming ... Get more on HelpWriting.net ...
  • 35. A Diagnostic Tool For Renal Disease And Hypertensive... Introduction Proteinuria is often used to detect the presence of any type of proteins in the urine and serves as a diagnostic tool for renal disease and hypertensive disorders. Among those disorders, pre– eclampsia is characterized by low renal perfusion and leakage of proteins in urine due to a damaged glomerular base membrane (1). This condition is associated with a high risk of maternal complications and prenatal mortality. Pre–eclampsia is usually defined as a combination of hypertension, edema and proteinuria after 20 weeks of gestation and is determined by a threshold of 300mg/24 hours of proteins (2). Normal urine excretion is up to 150mg/day (3). The most common test used in antenatal care to screen proteinuria is the visual reagent dipstick. It is a semi– quantitative and colorimetric method. The stick changes colour when the reagent (tetrabromophenol) binds to the proteins. The binding is pH dependent (albumin binds at a pH between 5 and 7, other proteins bind only at a pH below). If the urinalysis by the dipstick is positive, a 24 hour–urine collection is performed in order to confirm the proteinuria and to estimate the total amount of protein (4). Another method available for detecting proteinuria is called the Bradford assay. This quantitative method is based on the binding of proteins with the dye Coomassie Blue which increases the absorption of the dye at 595nm. The absorbance measured by spectrophotometry is related to the amount of proteins present in the ... Get more on HelpWriting.net ...
  • 36. Marketing Environment Analysis Of Wm Morrison Supermarkets... Introduction This assignment, as part of the Marketing module examination, will first include a marketing environment analysis of Wm Morrison Supermarkets PLC for both the internal and external environment of the company. Secondly, a SWOT (Strengths, Weaknesses, Opportunities and Threats) analysis will be conducted followed by strategic recommendations concerning the company's future operation. Prior to the above–mentioned contents of this assignment is a brief description of Wm Morrison Supermarkets PLC's profile. Last but not least, a paragraph at the end of this assignment will conclude all of its contents. Morrisons Brief Description Morrisons is a chain of supermarkets, currently being the 4th largest in the UK with a market share of 11% (Butler, 2014). It is a Public Limited Company whose legal name is Wm Morrison Supermarkets plc. The company's headquarters are located in Bradford, England (Morrisons, 2014a). Morrisons had 127,403 employees as at 02/02/2014 (Keynote, 2014), more than 500 stores (Morrisons, 2014d) and over 100 convenience stores (Morrisons, 2014b). Τhe company's main strategy is to capture growth and market share, increase its efficiency and finally remain and maybe improve its position in the top 4 supermarkets' list (along with Tesco, Sainsbury's and Asda) (Businessweek, 2014). In addition, Morrisons tries to buy local products when possible and in many cases manufactures products at its own sites (Morrisons, 2014c). Marketing ... Get more on HelpWriting.net ...
  • 37. Health And Safety At Work Act Health and safety at work act The health and safety at work act is a law which was put in place in 1974, this is to make sure that the people at work are looked after in the correct way and make sure that the work environment is safe. Also to ensure that they don't work in dangerous conditions and they use the right clothing and safety equipment. They have to make sure that they check the materials used and that they are stored and handled properly. They have to have a written safety/risk assessment form for the workplace. This is for everyone within the workplace including employers, suppliers, self–employed, trainees and manufactures. The health and safety at work act also ensure that the health and safety of others, for example the ... Show more content on Helpwriting.net ... This may cover an incident in which the work equipment, environment and activities were carried out, organised or supervised, lead in some way to the accident that happened. The reports which is filled out will alert the enforcing authorities to the incident which happened, depending on how serious the circumstances are the enforcing authorities will decide if it is suitable to investigate. Sporting example: When using the gym equipment they have to carry out an induction so that everyone is using the gym equipment safely and correctly. If they don't use it properly they could easily hurt themselves or other people around them. First aid at work act The health and safety (first aid) act ensures employers supply appropriate equipment, facilities and personnel for example a first aid box which includes plasters, bandages, medical tape etc, and staff are first aid trained. It depends on how serious the incident is in the workplace, this will determine on the how much first aid is needed. These regulations apply to all workplaces, these also include those with less than 5 employees and the self–employed. Sporting example: When Fabrice Muamba collapsed on the field during a football match, there were people that were first aid trained, so they could treat him and get him the help he needed quickly. His circumstances could have been a lot worse if there wasn't anyone ... Get more on HelpWriting.net ...
  • 38. William Bradford Research Paper "'Let's hurry back to the 'Mayflower' and bring our families ashore, William declared. But when they reached the 'Mayflower,' one person was missing. While they were gone, Dorothy Bradford had fallen overboard. She had been too weak to save herself. Once more, William was alone." (pg. 176– 177) Despite all the hardships that were thrown at him during his life, William Bradford, still became a remarkable, well–known man. When many of us would be asked if we knew who William Bradford was, we would quickly respond saying he was a great leader all throughout his life. Although, what hardships in life and experiences did he have when he was a boy, which transformed him into to such a greatly known man. Bradford Smith's Book, "William Bradford Pilgrim ... Show more content on Helpwriting.net ... This made him rely on God all throughout his life when he was alone, discouraged and sad. God comforted him in many ways by sending him friends at all different times and by simply giving him a peace inside. All these smalls challenges and tragedies in life, which God made him face, were only little tests of hardship to get him ready to rely on God even more in the upcoming years of the travel to America. . As Romans 8:28 says, "We know that in all things God works for the good of those who love him, who have been called according to his purpose." Even though in William's brain he thought he had a horrible, stressful childhood. He needed know that God had a plan the whole time and if God would have revealed that whole plan to him, William would have been happy and amazed with all he did in his life. For all the deaths of his family members, which made him rely on God spiritually. He was raised up strong, spiritually and physically which prepared him for making the American settlement by tilling the fields, farm work and walking those miles to and from, church and school. When he almost died in sickness but it was really getting him ready for the horrible sickness that awaited him in the trip to America. And all the miles he walked, even in the hard winter, to school every morning and night on his own accord, made it so that he was such a great leader, which got him to be the governor of ... Get more on HelpWriting.net ...
  • 39. Langthwaite Short Walk Beginning from the cosy village of Langthwaite, this short walk has outstanding views. Fine heather moorland and sparkling streams help to compensate for the ravages of the lead mines and spoil heaps. Langthwaite is acknowledged as the capital of Arkengarthdale and lies on the road from Reeth to Tan Hill. The road was constructed in c.1741 and it was one of the first turnpikes. Its original purpose was to transport coal from the Tan Hill mines to be used in the smelting furnaces around Langthwaite. Turnpike roads extracted tolls from travellers in order to pay for their building and maintenance. They often took advantage of earlier routes, especially through valleys, which were formerly toll free. Turnpikes were resented by the packhorse and ... Show more content on Helpwriting.net ... Bell pits were one of the earliest methods of mining for lead and other minerals. A vertical shaft was sunk to a depth of about 20 feet (6m) before digging horizontally, along the vein. The ore and waste were removed by a hand winch and a bucket. Due to poor ventilation, these workings could not progress very far before the air became foul. There was also the danger of the tunnel collapsing. To prevent this happening another bell pit was sunk close by. This procedure was repeated many times, resulting in a line of bell pits spaced out along the length of the vein. During our descent to Booze the scenery of Calver Hill, Fremington Edge and lower Arkengarthdale rouses the senses. Booze is a small hamlet, consisting of just a few farms and cottages, but unfortunately it does not have any licenced premises as its name implies. As a matter of fact, most of its early residents were Methodists and so they abstained from the demon drink. In 1473 the hamlet was known as Bowehouse, changing through the years to Booze. The original name means 'The house by the curve', the curve possibly referring to the curving hillside or perhaps to one of the nearby ... Get more on HelpWriting.net ...
  • 40. Tim Tebow's Strength is Jesus Christ ¨When people see the work ethic, determination and the passion that I have, they're going to ask, "What drives you? What is that?" Well, the answer is my relationship with Jesus Christ. that's just what has always fueled me." (Rogeberg). As a tremendously skilled and dedicated athlete, Tim Tebow uses his strengths to show Christ to people in a unique way. Tim Tebow demonstrates that Christlikeness and courage are essential to being a strong leader. On a crisp summer day, August the fourteenth, nineteen–eighty seven, a legend in football and leader in Christ was born, named Timothy Richard Tebow. He was born into a small town in the Philippines called Makati City. Tim Tebow's parents were originally from America but they moved to the Philippines to do baptist missionary work (Whitaker, 5). In October of 1990 Tim Tebow's family of four other older siblings moved to Jacksonville, Florida where Tim was raised. The Tebow's next door neighbor, Uncle Dick, was like a true uncle to Tim. Most of his childhood memories were spent in Uncle Dick's household (Whitaker, 7). You rarely see homeschoolers turn into NFL stars, but Tim Tebow was one. His mother homeschooled him from kindergarten until senior year of high school. His mother and father were his biggest influence to getting to know Christ ("Tim Tebow Biography"). Tim Tebow says when he was seven years old he had a strange fear of getting into a wreck and not spending eternity with God. With his parents help he accepted Christ ... Get more on HelpWriting.net ...
  • 41. Identifying An Organism With A Biological Lens Essay Spectrophotometrically Identifying Alfalfa Sprouts' Chloroplasts and Lysosomes Isolated in a Sucrose Gradient Introduction: Conceptualizing an organism with a biological lens involves understanding the organism's components and how their individual functions characterize the overarching entity. For a body, this means understanding organs and tissues. For a cell, this means classifying its organelles and discerning their functions. Analysis of subcellular components has become a major focus in biochemical studies, and it relies heavily on the fractionation of organelles. Fractioning and isolating organelles essentially enables a scientist to elucidate organelle functions, which, as mentioned before, adds definition to the all–encompassing image of a cell. Modern studies on this topic tend to target organelles with somewhat ambiguous functions, like anammoxosomes and melanosomes. By isolating these subcellular components and analyzing them, biologists have unearthed ammoxosomes' role in filling a large part of the Earth's atmosphere with nitrogen gas (Neumann et. al, 2014). In addition to this, other biological organizations have managed to sequester intact melanosomes from cells, separating them from other items of similar densities, and study the activity of V–Type ATPase proteins while they are in a subcellular membrane (Pelkonen et. al, 2016). The general procedure for executing an experiment like these involves lysing cells, centrifuging their components, and separating ... Get more on HelpWriting.net ...
  • 42. Wal Mart As A Positive Image Sam Walton started Wal–Mart in 1962. The first store was in Rogers Arkansas. Daft (2008) have a statement to Wal–Mart that, "Wal–Mart is the largest retailer in the United States" (p. 129). Wal– Mart's website and financial statements disclose that the key values and beliefs include Wal–Mart's claim to keep costs low for the customer and to build trust in its customer base ("Wal–Mart 2010 annual report", p. 16). Walton built Wal–Mart on three core values, including respect for the Individual, service to Our Customers, and striving for Excellence ("Wal–Mart statement of ethics", 2010 p. 5). Definitely, Wal–Mart has created a positive image through its several years' contributions. The paper will dis–cuss Wal–Mart's work in following ... Show more content on Helpwriting.net ... Walton once said that "a promise we make is a promise we keep," because they think the reason of provid–ing superior customer service is to assure that Wal–Mart are so thankful about consumers' trust and want to build a long relationship with customers. That is why they are always doing so. We can also see the respect of privacy in the Wal–Mart's privacy policy. Their policy in–dicates that how and why Wal–Mart uses, and shares personal information. For instance, when consumers take an order online, Wal–Mart automatically collects personal infor–mation, including name, address, phone number, card information. In this technology–boosting world, they recognize the importance of keep the safety of customer's personal information, because the information is so cheap. From the privacy policy page, we can know that they have lots of people who take charge to protect the information. When consumers are shopping on websites, or in the local stores, they use reasonable security methods. These methods include security access controls or other physical security safeguards, information security technologies and policies, procedures to help ensure the appropriate disposal of information (How Do We Secure Your Person–al Information, n.d). There are several examples, indicating that Wal–Mart pays a lot of attention on protecting personal information. For instance, ... Get more on HelpWriting.net ...