Preparation method of chitosan from shrimp shell

1. Preparation
of
Chitosan

2. Purified Chitin
55 % NaOH Solution
Water bath at 95
°C for 4 hours
Chitosan
Preparation of Chitosan

3. Chitosan Preparation

4. Chitosan Preparation

5. Chitosan Preparation

6. Chitin
Adding 50% NaOH
Boiled at 100°C for 2 hours
Washed continuously with the
50% NaOH and filtered
Chitosan
Deacetylation

7.  Chitosan nanofibers were also produced by the
electrospinning of chitin solutions using
1,1,1,3,3,3- hexafluoro-2-propanol (HFIP) as
the spinning solvent.
 Fiber diameters ranged between 50 and 460 nm,
but most fibers were smaller than 200 nm.
Electrospinning

8. Preparation of Chitosan
i. The chitin (10g) was put into 50% NaOH at
60°C for 8h to prepare crude chitosan.
ii. After filtration, the residue was washed with
hot distilled water at 60°C for three times.
iii. The crude chitosan (4.1g) was obtained by
drying in an air oven at 50°C overnight.

9. Preparation of Chitosan
I. Chitin is treated with 30 ml of 40% sodium
hydroxide solution at 105 C for 60, 90 and
120 min, respectively.
II.After filtration, washing to neutral with
deionized water and freeze drying, the
corresponding chitosan obtained

10. Nanowhisker a type of filamentary crystal (whisker) with
cross sectional diameter ranging from 1 to 100 nm and length
to diameter ratio >100.
Nanowhisker
 Needle or short rod-like structure
 Average length of 250 nm
 It can be several dozen times stronger
than regular crystals and can
demonstrate considerable flexibility
and corrosion resistance.

11.  Chitin nanocrystals are prepared from chitin
powder by using acid hydrolysis with sulfuric
acid and phosphoric acid.
 The prepared nanocrystals were characterized
and compared with nanocrystals prepared by
conventional HCl hydrolysis.
Nanocrystal